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survival of enterococcus faecalis during alkaline stress: changes in morphology, ultrastructure, physiochemical properties of the cell wall and specific gene transcripts.the aim of this investigation was to study the biochemical mechanisms employed by the endodontic pathogen enterococcus faecalis to survive alkaline environment during biofilm formation.201324112733
antimicrobial activity and sar study of new gemini imidazolium-based chlorides.a series of 70 new 3,3'(α,ω-dioxaalkyl)bis(1-alkylimidazolium) chlorides were synthesized. they were characterized with respect to surface active properties and antimicrobial activity against the following pathogens: staphylococcus aureus, enterococcus faecalis, acinetobacter baumannii, escherichia coli, klebsiella pneumoniae, enterobacter cloacae, pseudomonas aeruginosa, candida krusei, and candida albicans. in this article, besides description of the synthesis, we characterize a set of feature ...201424112802
hydrolysates of glycated and heat-treated peanut 7s globulin (ara h 1) modulate human gut microbial proliferation, survival and adhesion.evaluation of an effect of glycation of ara h 1 on proliferation and survival rate and adhesion of intestinal enterococcus faecalis, escherichia coli and lactobacillus acidophilus.201424118877
antimicrobial activity of short- and medium-term applications of polyhexamethylene biguanide, chlorhexidine digluconate and calcium hydroxide in infected immature bovine teeth in vitro.to compare the antimicrobial activity of polyhexamethylene biguanide (prontosan wound gel, pr) and chlorhexidine digluconate (chx) after short- and medium-term application with the disinfection ability of calcium hydroxide (ca) in a model using immature bovine teeth.201424118966
a new plant-derived antibacterial is an inhibitor of efflux pumps in staphylococcus aureus.an in-depth evaluation was undertaken of a new antibacterial natural product (1) recently isolated and characterised from the plant hypericum olympicum l. cf. uniflorum. minimum inhibitory concentrations (mics) were determined for a panel of bacteria, including: meticillin-resistant and -susceptible strains of staphylococcus aureus, staphylococcus epidermidis and staphylococcus haemolyticus; vancomycin-resistant and -susceptible enterococcus faecalis and enterococcus faecium; penicillin-resistan ...201324119569
polymerase chain reaction for enterococcus faecalis in drain fluid: the first screening test for symptomatic colorectal anastomotic leakage. the appeal-study: analysis of parameters predictive for evident anastomotic leakage.with current diagnostic methods, the majority of patients with symptomatic colorectal anastomotic leakage(cal) is identified approximately 1 week after operation.the aim of this study is to determine whether real-time polymerase chain reaction (rt-pcr) for detection of escherichia coli and enterococcus faecalis on drain fluid can serve as a screening test for cal in the early postoperative phase.201424122105
pestalotiopens a and b: stereochemically challenging flexible sesquiterpene-cyclopaldic acid hybrids from pestalotiopsis sp.from the endophytic fungus pestalotiopsis sp. isolated from the leaves of the chinese mangrove, rhizophora mucronata, two novel hybrid sesquiterpene-cyclopaldic acid metabolites with an unusual carbon skeleton, named pestalotiopens a and b, were obtained, together with the already known phytotoxin altiloxin b. pestalotiopen b even contains a third, triketide-derived module. the constitutions and the absolute configurations of the new metabolites and of altiloxin b were unambiguously determined b ...201324123472
a zn-dependent metallopeptidase is responsible for sensitivity to lsbb, a class ii leaderless bacteriocin of lactococcus lactis subsp. lactis bgmn1-5.lactococcus lactis subsp. lactis bgmn1-5 produces a leaderless class ii bacteriocin called lsbb. to identify the receptor for lsbb, a cosmid library of the lsbb-sensitive strain bgmn1-596 was constructed. about 150 cosmid clones were individually isolated and transferred to lsbb-resistant mutants of bgmn1-596. cosmid pazilcos/mn2, carrying a 40-kb insert, was found to restore lsbb sensitivity in lsbb-resistant mutants. further subcloning revealed that a 1.9-kb fragment, containing only one open ...201324123824
combined dielectrophoresis-raman setup for the classification of pathogens recovered from the urinary tract.rapid and effective methods of pathogen identifications are of major interest in clinical microbiological analysis to administer timely tailored antibiotic therapy. raman spectroscopy as a label-free, culture-independent optical method is suitable to identify even single bacteria. however, the low bacteria concentration in body fluids makes it difficult to detect their characteristic molecular fingerprint directly in suspension. therefore, in this study, raman spectroscopy is combined with diele ...201324125497
isolation and biological activities of an endophytic mortierella alpina strain from the antarctic moss schistidium antarctici.the antarctic endophytic fungus (strain ita1-ccma 952) was isolated from the moss schistidium antarctici found in admiralty bay, king george island, antarctica. strain ita1-ccma 952 was assigned to the specie mortierella alpina by phylogenetic analysis based on 18s rrna gene sequences. this strain produces high levels of polyunsaturated fatty acids (pufas), including y-(gamma) linolenic acid and arachidonic acid, which when combined represents 48.3% of the total fatty acid content. fungal extrac ...201424126742
survival, distribution, and translocation of enterococcus faecalis and implications for pregnant mice.pregnant mothers are susceptible to bacterial infections, which may compromise the health of mothers and offspring. enterococcus faecalis is a ubiquitous species found in food, restaurants, and hospitals where pregnant woman frequently become exposed to this bacterium. however, the survival, distribution, translocation, and corresponding influence of e. faecalis have not been investigated during the pregnancy period, when the mother and fetus are susceptible to bacterial infection. in this study ...201324131236
antibacterial, physicochemical and mechanical properties of endodontic sealers containing quaternary ammonium polyethylenimine nanoparticles.to evaluate the antibacterial, physicochemical and mechanical properties of two endodontic sealers incorporating quaternary ammonium polyethylenimine (qpei) nanoparticles at concentrations of 1% and 2% (w/w).201424134748
in vivo quantitative evaluation of live and dead bacteria in root canal infection by using propidium monoazide with real-time pcr.for selective detection of viable bacteria with molecular methods, propidium monoazide (pma) treatment has been successfully applied to a wide range of bacteria. the purpose of this study was to compare the quantity of live cells with the total amounts of both live and dead cells before and after chemomechanical preparation by using pma in combination with real-time polymerase chain reaction (qpcr).201324139254
effectiveness of antibiotic medicaments against biofilm formation of enterococcus faecalis and porphyromonas gingivalis.in this study we compared the antibacterial effect of triple antibiotic paste (tap), double antibiotic paste (dap), and calcium hydroxide [ca(oh)2] against enterococcus faecalis and porphyromonas gingivalis biofilm.201324139259
effect of smear layer against disinfection protocols on enterococcus faecalis-infected dentin.this study examined the effect of the smear layer on the antibacterial effect of different disinfecting solutions in infected dentinal tubules.201324139261
antimicrobial substantivity of alexidine and chlorhexidine in dentin.the use of irrigating solutions with long-lived activity helps to avoid failure of endodontic therapy. the aim of the present study was to evaluate and compare the antimicrobial substantivity against enterococcus faecalis by using as carrier a dentin-volumetric unit exposed to chlorhexidine (chx) and alexidine (alx).201324139264
effect of modified nonequilibrium plasma with chlorhexidine digluconate against endodontic biofilms in vitro.nonequilibrium plasma has been reported to effectively kill enterococcus faecalis in endodontic biofilm compared with chlorhexidine digluconate (chx). the purpose of this study was to evaluate the antimicrobial in vitro activity of modified nonequilibrium plasma with chx against e. faecalis and multispecies biofilms on bovine dentin discs.201324139270
nonclinical and clinical enterococcus faecium strains, but not enterococcus faecalis strains, have distinct structural and functional genomic features.certain strains of enterococcus faecium and enterococcus faecalis contribute beneficially to animal health and food production, while others are associated with nosocomial infections. to determine whether there are structural and functional genomic features that are distinct between nonclinical (nc) and clinical (cl) strains of those species, we analyzed the genomes of 31 e. faecium and 38 e. faecalis strains. hierarchical clustering of 7,017 orthologs found in the e. faecium pangenome revealed ...201424141120
activities of fosfomycin and rifampin on planktonic and adherent enterococcus faecalis strains in an experimental foreign-body infection model.enterococcal implant-associated infections are difficult to treat because antibiotics generally lack activity against enterococcal biofilms. we investigated fosfomycin, rifampin, and their combinations against planktonic and adherent enterococcus faecalis (atcc 19433) in vitro and in a foreign-body infection model. the mic/mbclog values were 32/>512 μg/ml for fosfomycin, 4/>64 μg/ml for rifampin, 1/2 μg/ml for ampicillin, 2/>256 μg/ml for linezolid, 16/32 μg/ml for gentamicin, 1/>64 μg/ml for va ...201424145537
the daf-16/foxo transcription factor functions as a regulator of epidermal innate immunity.the caenorhabditis elegans daf-16 transcription factor is critical for diverse biological processes, particularly longevity and stress resistance. disruption of the daf-2 signaling cascade promotes daf-16 activation, and confers resistance to killing by pathogenic bacteria, such as pseudomonas aeruginosa, staphylococcus aureus, and enterococcus faecalis. however, daf-16 mutants exhibit similar sensitivity to these bacteria as wild-type animals, suggesting that daf-16 is not normally activated by ...201324146615
in vitro comparison of the effectiveness of chlorhexidine and two calcium hydroxide formulations on enterococcus faecalis.the aim of this in vitro study was to compare the effectiveness of three intracanal medicaments in disinfecting the root canal and dentin of experimentally infected human teeth with enterococcus faecalis (ef).200824146671
photocatalytic antibacterial effects are maintained on resin-based tio2 nanocomposites after cessation of uv irradiation.photocatalysis induced by tio2 and uv light constitutes a decontamination and antibacterial strategy utilized in many applications including self-cleaning environmental surfaces, water and air treatment. the present work reveals that antibacterial effects induced by photocatalysis can be maintained even after the cessation of uv irradiation. we show that resin-based composites containing 20% tio2 nanoparticles continue to provide a pronounced antibacterial effect against the pathogens escherichi ...201324146793
abdominal cerebrospinal fluid pseudocyst due to infection with enterococcus faecalis. a case report.abdominal cerebrospinal fluid pseudocyst is a rare complication of ventriculo-peritoneal shunt occurring as a result of non-absorption of cerebrospinal fluid from the abdominal cavity due to inflammation. usual presentations include abdominal symptoms; abdominal distention, pain, nausea, vomiting and symptoms of raised intracranial pressure like headache due to shunt dysfunction. the diagnosis may be delayed in severely handicapped patients due to poor communicability, multiple associated compli ...201024148340
colon macrophages polarized by commensal bacteria cause colitis and cancer through the bystander effect.intestinal commensal bacteria have recently been shown to trigger macrophages to produce diffusible clastogens (or chromosome-breaking factors) through a bystander effect (bse) that mediates dna damage and induces chromosomal instability in neighboring cells. colon macrophages appear central to colon carcinogenesis and bse through the expression of tumor necrosis factor-α (tnf-α) and cyclooxygenase-2 (cox-2). the former induces netrin-1, a regulator of intestinal epithelial cell apoptosis, and t ...201324151540
adhesion and virulence factor properties of enterococci isolated from clinical samples in iran.enterococci rank among leading causes of nosocomial bacteremia, urinary tract infections and community acquired endocarditis. the aim of the present study was to investigate the presence of virulence factors in enterococci strains isolated from clinical samples in iranian educational hospitals.201324152500
evaluation of the antibacterial efficacy of omeprazole with sodium hypochlorite as an endodontic irrigating solution- an invivo study.proton pump inhibitors are used in endodontic disinfection of root canals for elimination of enterococcus faecalis. this invivo study on wistner rats is carried out to determine antimicrobial efficacy of proton pump inhibitor in combination with sodium hypochlorite & mixture of isomer of tetracycline, acid and detergent (mtad) against e. faecalis materials & methods: periapical lesions were induced on the 30 incisor teeth of 30 male wistner rats (10per group). after 28 days, root canals of each ...201324155586
expression of the agmatine deiminase pathway in enterococcus faecalis is activated by the agur regulator and repressed by ccpa and pts(man) systems.although the agmatine deiminase system (agdi) has been investigated in enterococcus faecalis, little information is available with respect to its gene regulation. in this study we demonstrate that the presence of exogenous agmatine induces the expression of agu genes in this bacterium. in contrast to the homologous and extensively characterized agdi system of s. mutants, the agubdac operon in e. faecalis is not induced in response to low ph. in spite of this, agmatine catabolism in this bacteriu ...201324155893
the impact of inappropriate antibiotics on bacteremia patients in a community hospital in taiwan: an emphasis on the impact of referral information for cases from a hospital affiliated nursing home.evidence for the impact of inappropriate antimicrobial therapy on bacteremia is mainly from studies in medical centers. we investigated the impact of inappropriate antimicrobial therapy on bacteremia in a community hospital. in particular, patients from the hospital's affiliated nursing home were sent to the hospital with adequate referral information.201324156241
prevalence and antimicrobial resistance of canine urinary tract pathogens.this study aims to describe the incidence and risk factors for positive urinary tract culture, the prevalence of urinary tract pathogens in single organism and mixed cultures and changes in their antimicrobial resistance over 10 years. a retrospective review of computer records detailing canine urine samples submitted between august 1999 and september 2009 for culture and sensitivity in a uk tertiary referral hospital is described. 17.5 per cent of 5923 samples (670 of 4530 dogs) were positive c ...201324158327
bio-preservation of ground beef meat by enterococcus faecalis cect7121.meat and particularly ground beef is frequently associated with food poisoning episodes and breeches in food safety. the main goal of this research was to evaluate the bactericide effect of the probiotic enterococcus faecalis cect7121, against different pathogens as: escherichia coli o157:h7, staphylococcus aureus, clostridium perfringens and listeria monocytogenes, inoculated in ground beef meat. three studies were performed to evaluate the inhibition of e. faecalis cect7121 on ground beef meat ...201324159282
[treatment of endocarditis and spondylitis caused by enterococcus faecalis with continuous intravenous infusion of ampicillin]. 201224159662
does cmv infection impact the virulence of enterococcus faecalis? 201324162854
a new antibacterial amino phenyl pyrrolidone derivative from a novel marine gliding bacterium rapidithrix thailandica.a recently described marine gliding bacterium rapidithrix thailandica strain tistr 1741 was isolated from biofilm specimen collected from the andaman sea in thailand. four liters fermentation broth of r. thailandica tistr 1741 cultivated in vy/2 medium were extracted with methanol to yield a novel amino phenyl pyrrolidone derivative compound (1) with antibacterial activities. the chemical structure and physico-chemical properties of 1 were investigated by spectrometry techniques. compound 1 exhi ...201424162950
inefficient translation renders the enterococcus faecalis fabk enoyl-acyl carrier protein reductase phenotypically cryptic.enoyl-acyl carrier protein (acp) reductase catalyzes the last step of the bacterial fatty acid elongation cycle. enterococcus faecalis is unusual in that it encodes two unrelated enoyl-acp reductases, fabi and fabk. we recently reported that deletion of the gene encoding fabi results in an unsaturated fatty acid (ufa) auxotroph despite the presence of fabk, a gene encoding a second fully functional enoyl-acp reductase. by process of elimination, our prior report argued that poor expression was t ...201424163335
antimicrobial activity and cytotoxicity of the ethanol extract, fractions and eight compounds isolated from eriosema robustum (fabaceae).the aim of this study was to evaluate the antimicrobial activity and the cytotoxicity of the ethanol crude extract, fractions and isolated compounds from the twigs of eriosema robustum, a plant used for the treatment of coughs and skin diseases.201324165199
vancomycin-resistant enterococci among haemodialysis patients in portugal: prevalence and molecular characterization of resistance, virulence and clonality.vancomycin-resistant enterococci (vre) among haemodialysis patients has increased rapidly and, to date, there is no report of this incidence in portugal.201424169320
antimicrobial activity of three root canal irrigants on enterococcus faecalis: an in vitro study.the aim of this study was to compare the antimicrobial effects of 2.5% sodium hypochlorite (naocl), 2% chlorhexidine gluconate (chx) and biopure mtad (mtad) on enterococcus (e) faecalis-contaminated root canals of human extracted teeth.200824171016
antimicrobial efficacy of mineral trioxide aggregate with and without silver nanoparticles.most current root-end filling materials do not provide a perfect seal. thus, a microscopic space is likely to exist in the interface between walls of the root-end cavity and filling material, which allows microorganisms and their products to penetrate. in addition to good sealing ability and biocompatibility, root-end filling materials should ideally have some antimicrobial activity. therefore, this in vitro study aimed to evaluate the antimicrobial properties of angelus white mineral trioxide a ...201324171023
calcium enriched mixture and mineral trioxide aggregate activities against enterococcus faecalis in presence of dentin.the purpose of this in vitro study was to compare the antibacterial activity of calcium enriched mixture (cem) with proroot mineral trioxide aggregate (mta) against enterococcus faecalis (e. faecalis) in the presence/absence of dentin powder.201324171028
in vitro evaluation of the minimum bactericidal concentrations of different root-end filling materials.the aim of this study was to evaluate the minimum bactericidal concentrations (mbc) of root-end filling materials proroot mta, mta angelus and irm.201324171975
antimicrobial efficacy of octenidine hydrochloride, mtad and chlorhexidine gluconate mixed with calcium hydroxide.the aim of this in vitro study was to investigate whether mixing with calcium hydroxide [ca(oh)2] affects the antimicrobial action of octenidine hydrochloride (octenisept), mtad and chlorhexidine against enterococcus faecalis and candida albicans.201324171989
rapid whole-genome sequencing for detection and characterization of microorganisms directly from clinical samples.whole-genome sequencing (wgs) is becoming available as a routine tool for clinical microbiology. if applied directly on clinical samples, this could further reduce diagnostic times and thereby improve control and treatment. a major bottleneck is the availability of fast and reliable bioinformatic tools. this study was conducted to evaluate the applicability of wgs directly on clinical samples and to develop easy-to-use bioinformatic tools for the analysis of sequencing data. thirty-five random u ...201424172157
inducing secondary metabolite production by the endophytic fungus fusarium tricinctum through coculture with bacillus subtilis.coculturing the fungal endophyte fusarium tricinctum with the bacterium bacillus subtilis 168 trpc2 on solid rice medium resulted in an up to 78-fold increase in the accumulation in constitutively present secondary metabolites that included lateropyrone (5), cyclic depsipeptides of the enniatin type (6-8), and the lipopeptide fusaristatin a (9). in addition, four compounds (1-4) including (-)-citreoisocoumarin (2) as well as three new natural products (1, 3, and 4) were not present in discrete f ...201324175613
ammonia sanitisation of sewage sludge using urea.the aim of the study was to develop a simple, low-cost treatment for sewage sludge using urea as a sanitising agent. sewage sludge was spiked with enterococcus faecalis and salmonella typhimurium, treated with 0.5, 1, 1.5 and 2% w/w urea at laboratory scale, and the viability was monitored during 4 months of storage at 4, 10 and 22 °c (only 0.5%). a linear relationship was identified between salmonella spp. inactivation rate and ammonia (nh3) concentration. temperature had a positive impact on s ...201324185072
sunlight mediated inactivation mechanisms of enterococcus faecalis and escherichia coli in clear water versus waste stabilization pond water.escherichia coli and enterococci have been previously reported to differ in the mechanisms and conditions that affect their sunlight-mediated inactivation in waste stabilization ponds. this study was undertaken to further characterize these mechanisms, using simulated sunlight and single strains of laboratory-grown e. coli and enterococcus faecalis, with a focus on characterizing the contribution of exogenous reactive oxygen species to the inactivation process. we found that direct damage by uvb ...201424188579
focal targeting by human β-defensin 2 disrupts localized virulence factor assembly sites in enterococcus faecalis.virulence factor secretion and assembly occurs at spatially restricted foci in some gram-positive bacteria. given the essentiality of the general secretion pathway in bacteria and the contribution of virulence factors to disease progression, the foci that coordinate these processes are attractive antimicrobial targets. in this study, we show in enterococcus faecalis that seca and sortase a, required for the attachment of virulence factors to the cell wall, localize to discrete domains near the s ...201324191013
microbiological findings of vulvovaginitis in prepubertal girls.to compare vaginal culture results between prepubertal girls with and without vulvovaginitis, and obtain an overview of the most commonly encountered microbes.201424191064
bactericidal efficacy of photodynamic therapy against enterococcus faecalis in infected root canals: a systematic literature review.the aim was to review the bactericidal efficacy of photodynamic therapy (pdt) against enterococcus faecalis (e. faecalis) in infected root canals.201324192536
measuring the effects of bacteria on c. elegans behavior using an egg retention assay.c. elegans egg-laying behavior is affected by environmental cues such as osmolarity and vibration. in the total absence of food c. elegans also cease egg-laying and retain fertilized eggs in their uterus. however, the effect of different sources of food, especially pathogenic bacteria and particularly enterococcus faecalis, on egg-laying behavior is not well characterized. the egg-in-worm (eiw) assay is a useful tool to quantify the effects of different types of bacteria, in this case e. faecali ...201324192811
prevalence of the aminoglycoside phosphotransferase genes aph(3')-iiia and aph(3')-iia in escherichia coli, enterococcus faecalis, enterococcus faecium, pseudomonas aeruginosa, salmonella enterica subsp. enterica and staphylococcus aureus isolates in austria.the aminoglycoside phosphotransferase aph(3')-iia primarily inactivates kanamycin and neomycin, whilst aph(3')-iiia also inactivates amikacin. the aim of this study was to determine the frequency of both resistance genes in major human pathogens to obtain their baseline prevalence in the gene pool of these bacterial populations in austria. in total, 10 541 escherichia coli, enterococcus faecalis, enterococcus faecium, pseudomonas aeruginosa, salmonella enterica subsp. enterica and staphylococcus ...201424194558
epidemic escherichia coli st131 and enterococcus faecium st17 in coastal marine sediments from an italian beach.fecal indicator bacteria (fib) are used worldwide to assess water quality in coastal environments, but little is known about their genetic diversity and pathogenicity. this study examines the prevalence, antimicrobial resistance, virulence, and genetic diversity of fib isolated from marine sediments from a central adriatic seaside resort. fib, recovered from 6 out of 7 sites, were significantly more abundant at sampling stations 300 m offshore than close to the shore. escherichia coli accounted ...201324195439
photoactivated rose bengal functionalized chitosan nanoparticles produce antibacterial/biofilm activity and stabilize dentin-collagen.treatment of infected teeth presents two major challenges: persistence of the bacterial-biofilm within root canals after treatment and compromised structural integrity of the dentin hard-tissue. in this study bioactive polymeric chitosan nanoparticles functionalized with rose-bengal, csrbnp were developed to produce antibiofilm effects as well as stabilize structural-integrity by photocrosslinking dentin-collagen. csrbnp were less toxic to fibroblasts and had significant antibacterial activity e ...201424200522
differences in visceral fat and fat bacterial colonization between ulcerative colitis and crohn's disease. an in vivo and in vitro study.crohn's disease (cd) is notably characterized by the expansion of visceral fat with small adipocytes expressing a high proportion of anti-inflammatory genes. conversely, visceral fat depots in ulcerative colitis (uc) patients have never been characterized. our study aims were a) to compare adipocyte morphology and gene expression profile and bacterial translocation in omental (om) and mesenteric (mes) adipose tissue of patients with uc and cd, and b) to investigate the effect of bacterial infect ...201324205244
phenotypic expression, molecular characterization and transferability of erythromycin resistance genes in enterococcus spp. isolated from naturally fermented food.to evaluate phenotypic resistance to macrolides-lincosamides and streptogramin b (mlsb ) antibiotics and to determine their localization as well as transferability of erythromycin resistance genes in enterococcal isolates of naturally fermented food-idli batter.201424206069
technological properties of bacteriocin-producing lactic acid bacteria isolated from pico cheese an artisanal cow's milk cheese.evaluate technologically relevant properties from bacteriocin-producing strains to use as starter/adjunct cultures in cheese making.201424206097
[necrotizing pneumonia due to enterococcus faecalis in an immunocompromised patient of 67 years]. 201324211001
lipoteichoic acid of streptococcus mutans interacts with toll-like receptor 2 through the lipid moiety for induction of inflammatory mediators in murine macrophages.streptococcus mutans is a pathogenic gram-positive bacterium that is closely associated with dental caries and subsequent pulpal inflammation. although lipoteichoic acid (lta) is considered a major virulence factor of gram-positive bacteria, little is known about the innate immunity to s. mutans lta. in this study, we purified lta from s. mutans (sm.lta) through n-butanol extraction, hydrophobic interaction column chromatography, and ion-exchange column chromatography to investigate its immunolo ...201424216318
[pathogens of prostatitis and their drug resistance: an epidemiological survey].to investigate the epidemiological features of the pathogens responsible for prostatitis in the changshu area, and offer some evidence for the clinical treatment of prostatitis.201324218946
breakthrough bacteremia by linezolid-susceptible enterococcus faecalis under linezolid treatment in a severe polytrauma patient. 201324222608
reply to "breakthrough bacteremia by linezolid-susceptible enterococcus faecalis under linezolid treatment in a severe polytrauma patient". 201324222609
rapid kill-novel endodontic sealer and enterococcus faecalis.with growing concern over bacterial resistance, the identification of new antimicrobial means is paramount. in the oral cavity microorganisms are essential to the development of periradicular diseases and are the major causative factors associated with endodontic treatment failure. as quaternary ammonium compounds have the ability to kill a wide array of bacteria through electrostatic interactions with multiple anionic targets on the bacterial surface, it is likely that they can overcome bacteri ...201324223159
microbiological etiology of bacterial prostatitis in general hospital and primary care clinic in korea.the national institutes of health classification of prostatitis reported the proportion of chronic bacterial prostatitis, especially category ii, at 3% to 10%. because of the polymerase chain reaction (pcr) diagnosis technique, chronic prostatitis syndrome (cps) with a known bacterial origin has increased recently. in this study, we evaluated the proportion of chronic bacterial prostatitis in a general hospital and a primary care clinic (pcc) in addition to the distribution of the microorganism ...201324223415
biofilm mediates enterococcus faecalis adhesion, invasion and survival into bovine mammary epithelial cells.we proposed in this study that during intramammary infection, biofilm formation may facilitate adherence and colonization of enterococcus faecalis to mammary gland epithelium. this was established by comparing six different ent. faecalis isolates with different biofilm-forming profiles for their adhesive, invasive and survival capabilities to bovine mammary epithelial cell line (mac-t). our results showed increased ability of the biofilm-producer ent. faecalis strains to adhere, invade and survi ...201424224825
false-positive pneumococcal antigen test in a case of enterococcus faecalis meningitis. 201324231250
role of intracellular stochasticity in biofilm growth. insights from population balance modeling.there is increasing recognition that stochasticity involved in gene regulatory processes may help cells enhance the signal or synchronize expression for a group of genes. thus the validity of the traditional deterministic approach to modeling the foregoing processes cannot be without exception. in this study, we identify a frequently encountered situation, i.e., the biofilm, which has in the past been persistently investigated with intracellular deterministic models in the literature. we show in ...201324232571
bacteriocin protein bacl1 of enterococcus faecalis is a peptidoglycan d-isoglutamyl-l-lysine endopeptidase.enterococcus faecalis strains are commensal bacteria in humans and other animals, and they are also the causative agent of opportunistic infectious diseases. bacteriocin 41 (bac41) is produced by certain e. faecalis clinical isolates, and it is active against other e. faecalis strains. our genetic analyses demonstrated that the extracellular products of the bacl1 and baca genes, which are encoded in the bac41 operon, coordinately express the bacteriocin activity against e. faecalis. in this stud ...201324235140
characterization of enterococcus faecalis phage ime-ef1 and its endolysin.enterococcus faecalis is increasingly becoming an important nosocomial infection opportunistic pathogen. e. faecalis can easily obtain drug resistance, making it difficult to be controlled in clinical settings. using bacteriophage as an alternative treatment to drug-resistant bacteria has been revitalized recently, especially for fighting drug-resistant bacteria. in this research, an e. faecalis bacteriophage named ime-ef1 was isolated from hospital sewage. whole genomic sequence analysis demons ...201324236180
[expression of quorum-sensing related genes during enterococcus faecalis biofilm formation].to investigate the relationship between the expression of the quorum-sensing related genes during enterococcus faecalis(ef) biofilm formation.201324238415
antibacterial efficacy of a human β-defensin-3 peptide on multispecies biofilms.the aggregation of mixed bacterial flora into sessile biofilms on root canal surfaces can be one of the causes of persistent apical periodontitis. the aim of this study was to evaluate the antibacterial efficacy of human β-defensin-3 (hbd3) peptide on multispecies biofilms by using confocal laser scanning microscopy.201324238461
design, synthesis and biological evaluation of α-substituted isonipecotic acid benzothiazole analogues as potent bacterial type ii topoisomerase inhibitors.the discovery and optimisation of a new class of benzothiazole small molecules that inhibit bacterial dna gyrase and topoisomerase iv are described. antibacterial properties have been demonstrated by activity against dna gyrase atpase and potent activity against staphylococcus aureus, enterococcus faecalis, streptococcus pyogenes and haemophilus influenzae. further refinements to the scaffold designed to enhance drug-likeness included analogues bearing an α-substituent to the carboxylic acid gro ...201324239017
prgk, a multidomain peptidoglycan hydrolase, is essential for conjugative transfer of the pheromone-responsive plasmid pcf10.peptidoglycan (pg) hydrolases associated with bacterial type iv secretion systems (t4sss) are thought to generate localized lesions in the pg layer to facilitate assembly of the translocation channel. the pheromone-responsive plasmid pcf10 of enterococcus faecalis encodes a putative cell wall hydrolase, prgk, and here we report that a prgk deletion abolished functionality of the pcf10-encoded t4ss as monitored by pcf10 conjugative transfer. expression in trans of wild-type prgk fully complemente ...201424244005
the effectiveness of enzymic irrigation in removing a nutrient-stressed endodontic multispecies biofilm.to establish a nutrient-stressed multispecies model biofilm and investigate the dynamics of biofilm killing and disruption by 1% trypsin and 1% proteinase k with or without ultrasonic activation.201424246147
boosted rat natural xenoantibodies cross-react with enterococcus faecalis by targeting melibiose and l-rhamnose.natural antibodies include a subset described as xenoantibodies considered to be directed at microorganisms and also cross-react with antigens of unrelated species. in this study, we generated t-cell-independent (ti) and t-cell-dependent (td) xenoantibodies in lewis rats with hamster and pig blood injections. ti anti-hamster and anti-pig igm and igg xenoantibodies cross-reacted with enterococcus faecalis but not with escherichia coli isolated from the blood of lewis rats after cecal ligation and ...201424246417
friends group: clinical and microbiological characteristics of post-filtering surgery endophthalmitis.to study the clinical and microbiological characteristics as well as the prognostic factors for post-filtering surgery endophthalmitis.201424248809
comparison of bacteria isolated from emergency department patients versus hospitalized patients.the frequencies and corresponding susceptibilities of bacteria isolated from patients in the emergency department (ed) were compared with those from hospitalized patients.201324249762
evaluating the in-vitro antibacterial effect of iranian propolis on oral microorganisms.propolis has traditionally been used in curing infections and healing wounds and burns. current researches have shown that propolis has antibacterial, antifungal and antiviral actions however, the pharmacological activity of propolis is highly variable depending on its geographic origin. there have been few studies on the effects of iranian propolis on the oral microorganisms. in this in-vitro study, the antimicrobial activity of the ethanolic and water extracts of the iranian propolis (10%, w/v ...201124250366
immunostimulatory cpg motifs in the genomes of gut bacteria and their role in human health and disease.toll-like receptor (tlr) signalling plays an important role in epithelial and immune cells of the intestine. tlr9 recognizes unmethylated cpg motifs in bacterial dna, and tlr9 signalling maintains the gut epithelial homeostasis. here, we carried out a bioinformatic analysis of the frequency of cpg motifs in the genomes of gut commensal bacteria across major bacterial phyla. the frequency of potentially immunostimulatory cpg motifs (all cpg hexamers) or purine-purine-cg-pyrimidine-pyrimidine hexa ...201424255136
[surveillance of bacterial distribution and drug resistance in inpatients with surgical infections: a single center study].to investigate the bacterial distribution and drug resistance in patients with surgical infections, and provide the basis for the standardization treatment of the surgical infection.201324256585
a proposal for source tracking of fecal pollution in recreational waters by pulsed-field gel electrophoresis.this study aimed to identify specific river sources of fecal contamination by applying pulsed-field gel electrophoresis (pfge) to environmental water samples from a recreational beach in japan. the genotypes of all enterococcus faecium and enterococcus faecalis strains used as indicators of fecal pollution on the recreational beach and rivers were analyzed by pfge, and the pfge profiles of the strains were classified at a 0.9 similarity level using dendrogram analysis. pfge types of e. faecium i ...201324256972
antimicrobial activity of essential oils and chloroform alone and combinated with cetrimide against enterococcus faecalis biofilm.the enterococcus faecalis bacteria have been identified as the most commonly recovered species from teeth with persistent endodontic infections. the antimicrobial activity of essential oils and chloroform (chl), alone and in association with various concentrations of cetrimide (ctr), against biofilm of enterococcus faecalis was investigated. solutions of chl, eucalyptus oil (eo) and orange oil (oo) associated with ctr at 0.3%, 0.2%, 0.1%, and 0.05% were used to determine antimicrobial activity b ...201324265917
neutralization of the antimicrobial effect of glyphosate by humic acid in vitro.in the present study, the neutralization ability of the antimicrobial effect of glyphosate by different humic acids was investigated. the minimal inhibitory concentrations of glyphosate for different bacteria such as bacillus badius, bifidobacterium adolescentis, escherichia coli, e. coli 1917 strain nissle, enterococcus faecalis, enterococcus faecium, salmonella enteritidis and salmonella typhimurium were determined in the presence or absence of different concentrations of humic acid (0.25, 0.5 ...201424268342
investigation of a multiresistance gene cfr that fails to mediate resistance to phenicols and oxazolidinones in enterococcus faecalis.to investigate the basis of susceptibility to phenicols and oxazolidinones of the porcine enterococcus faecalis cppf5 despite the presence of the multiresistance gene cfr.201424272266
nutritional control of antibiotic resistance via an interface between the phosphotransferase system and a two-component signaling system.enterococci are ubiquitous inhabitants of the gastrointestinal (gi) tract. however, antibiotic-resistant enterococci are also major causes of hospital-acquired infections. enterococci are intrinsically resistant to cephalosporins, enabling growth to abnormally high densities in the gi tract in patients during cephalosporin therapy, thereby promoting dissemination to other sites where they cause infection. despite its importance, many questions about the underlying basis for cephalosporin resista ...201424277024
in vitro evaluation of the risk of inducing bacterial resistance to disinfection treatment with photolysis of hydrogen peroxide.the purpose of the present study was to evaluate the risk of inducing bacterial resistance to disinfection treatment with photolysis of h2o2 and comparing this with existing antibacterial agents. we tested seven antibacterial agents, including amoxicillin, cefepime hydrochloride, erythromycin, ofloxacin, clindamycin hydrochloride, ciprofloxacin hydrochloride, and minocycline hydrochloride, as positive controls for validation of the assay protocol. for all of the agents tested, at least one of th ...201324282582
effects of microgravity on the virulence of listeria monocytogenes, enterococcus faecalis, candida albicans, and methicillin-resistant staphylococcus aureus.to evaluate effects of microgravity on virulence, we studied the ability of four common clinical pathogens--listeria monocytogenes, methicillin-resistant staphylococcus aureus (mrsa), enterococcus faecalis, and candida albicans--to kill wild type caenorhabditis elegans (c. elegans) nematodes at the larval and adult stages. simultaneous studies were performed utilizing spaceflight, clinorotation in a 2-d clinorotation device, and static ground controls. the feeding rate of worms for killed e. col ...201324283929
dual defensin strategy for targeting enterococcus faecalis. 201324284170
surgical site infections among high-risk patients in clean-contaminated head and neck reconstructive surgery: concordance with preoperative oral flora.salivary contamination of surgical wounds in clean-contaminated head and neck surgery with free flap reconstruction remains a major cause of infection and leads to significant morbidity. this study investigates the correlation between intraoral flora and surgical site infections (ssis) among high-risk head and neck cancer patients undergoing resection and free flap reconstruction.201324284742
characterization of lactic acid bacteria isolated from infant faeces as potential probiotic starter cultures for fermented sausages.a total of 109 lactic acid bacteria isolated from infant faeces were identified by partial 16s rrna, cpn60 and/or phes sequencing. lactobacillus was the most prevalent genus, representing 48% of the isolates followed by enterococcus (38%). lactobacillus gasseri (21%) and enterococcus faecalis (38%) were the main species detected. a further selection of potential probiotic starter cultures for fermented sausages focused on lactobacillus as the most technologically relevant genus in this type of p ...201424290655
incidence of virulence factors in enterococci from raw and fermented meat and biofilm forming capacity at 25°c and 37°c.twenty-nine enterococcus strains from raw and fermented meat products were screened for the presence of virulence genes, including those for aggregation substances (asa1 and asa373), cytolysin activator (cyla), collagen binding protein (ace), endocarditis antigen (efaa), enterococcal surface protein (esp) and gelatinase (gele). virulence gene occurrence, expression of gelatinase and pheromone aggregation was greater in enterococcus faecalis than in enterococcus faecium strains. all e. faecalis a ...201424291183
molecular characterization of vancomycin-resistant enterococci in serbia: intensive care unit as the source.the purpose of this study was to evaluate the molecular relatedness of clinical isolates of vancomycin-resistant enterococci (vre) collected from patients of the clinic for infectious and tropical diseases in belgrade. among 40 isolates available for the investigation, 36 were identified as enterococcus faecium, whereas 2 were enterococcus faecalis and enterococcus raffinosus, respectively. pulsed-field gel electrophoresis (pfge) typing revealed 21 strain types, comprising 7 clusters which conta ...201324292087
diversity of lactic acid bacteria associated with fresh coffee cherries in taiwan.a total of 102 lactic acid bacteria (lab) were isolated from three different coffee farms in taiwan. these isolates were classified and identified by the restriction fragment length polymorphism analysis and sequencing of 16s ribosomal dna. heterofermentative leuconostoc, and weissella species were the most common lab found in two farms located at an approximate altitude of 800 m. lactococcus lactis subsp. lactis was the most common lab found in the remaining farm was located at an approximate a ...201424292770
decolourisation of acid orange 7 recalcitrant auto-oxidation coloured by-products using an acclimatised mixed bacterial culture.this study focuses on the biodegradation of recalcitrant, coloured compounds resulting from auto-oxidation of acid orange 7 (ao7) in a sequential facultative anaerobic-aerobic treatment system. a novel mixed bacterial culture, bac-zs, consisting of brevibacillus panacihumi strain zb1, lysinibacillus fusiformis strain zb2, and enterococcus faecalis strain zl bacteria were isolated from environmental samples. the acclimatisation of the mixed culture was carried out in an ao7 decolourised solution. ...201424293297
frequency of ace, epa and elra genes in clinical and environmental strains of enterococcus faecalis.surface proteins play an important role in the pathogenesis of enterococcal infections. some of them are candidates for a vaccine, e.g., the frequency of endocarditis in rats vaccinated with ace protein was 75 % as 12 opposed to 100 % in those who weren't. however, there are other components of enterococcal cells, such as epa antigens or internalin-like proteins, which may be used in the prophylaxis of infections caused by them. however, also other virulence factors and resistance to antibiotics ...201224293719
low metabolic activity of biofilm formed by enterococcus faecalis isolated from healthy humans and wild mallards (anas platyrhynchos).it is widely known that enterococcus faecalis virulence is related to its biofilm formation. although enterococci are common commensal organisms of the gastrointestinal tract, the difference between commensal and pathogen strains remain unclear. in this study, we compare the biochemical profile of the biofilms formed by two groups of medical and two groups of commensal strains. the medical strains were isolated as pathogens from infections of urinary tract and other infections (wounds, pus and b ...201324294189
biofilm formation on polystyrene under different temperatures by antibiotic resistant enterococcus faecalis and enterococcus faecium isolated from food.the ability of antibiotic resistant e. faecalis and e. faecium isolated from food to form biofilm at different temperatures in the absence or presence of 0.75% glucose was evaluated. a synergistic effect on biofilm at 10 °c, 28 °c, 37 °c and 45 °c and glucose was observed for e. faecalis and e. faecium.201324294231
quantifying the extruded bacteria following use of two rotary instrumentation systems.all instrumentation techniques have been reported to be associated with extrusion of infected debris. the aim of this study was to evaluate the number of bacteria extruded apically from extracted teeth ex vivo after canal instrumentation using the two engine-driven techniques utilizing nickel-titanium instruments (flex master and mtwo).200724298285
antibacterial activity of resin rich plant extracts.the in vitro antibacterial activity of resin rich methanolic extracts (rrmes) of commiphora myrrha, operculina turpethum, and pinus roxburghii.201324302834
comparison of peptide nucleic acid fluorescence in situ hybridization assays with culture-based matrix-assisted laser desorption/ionization-time of flight mass spectrometry for the identification of bacteria and yeasts from blood cultures and cerebrospinal fluid cultures.peptide nucleic acid fluorescence in situ hybridization (pna fish) is a molecular diagnostic tool for the rapid detection of pathogens directly from liquid media. the aim of this study was to prospectively evaluate pna fish assays in comparison with culture-based matrix-assisted laser desorption/ionization-time of flight mass spectrometry (maldi-tof ms) identification, as a reference method, for both blood and cerebrospinal fluid (csf) cultures, during a 1-year investigation. on the basis of the ...201424304149
antimicrobial efficacy of photodynamic therapy and sodium hypochlorite on monoculture biofilms of enterococcus faecalis at different stages of development.as the enterococcus faecalis biofilm ages, it is likely that bacteria in mature and old biofilms will become more resistant to commonly used antibacterial irrigants. photodynamic therapy (pdt) has been used to kill resistant bacteria organized in a biofilm. this study aimed to investigate the antimicrobial effectiveness of 1% sodium hypochlorite (naocl), 2.5% naocl, and pdt on e. faecalis biofilm at different stages of development.201424308707
virulence and antimicrobial resistance in enterococci isolated from urinary tract infections.urinary tract infection (uti) is the most common nosocomial infection among hospitalized patients. meanwhile, most frequent infections involving enterococci affect the urinary tract. the aims of this study were to investigate the susceptibility pattern of isolated enterococci from uti and the prevalence of virulence genes.201324312835
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