Publications

influence of sex hormones on coxsackie b-3 virus infection in balb/c mice.		1982	6280880
infection as a predominant cause of perinatal mortality.	during a 15-month period, all 34 infants delivered at the department of obstetrics and gynecology at university hospital in lund, sweden, who died perinatally or neonatally were included in a prospective study of causes of death. autopsies--including extensive culturing of specimens for bacteria, chlamydia, fungi, mycoplasmas, and viruses--were performed for all infants. maternal sera obtained during pregnancy and after delivery were examined regarding titers against a number of microorganisms. ...	1982	6281706
a solid-phase assay of solubilized hela cell membrane receptors for binding group b coxsackieviruses and polioviruses.		1982	6281966
induction of cytopathogenicity in mammalian cell lines challenged with culturable enteric viruses and its enhancement by 5-iododeoxyuridine.	cultures of 17 established cell lines were tested against 105 enteric virus types for capacity to support viral replication as indicated by cytopathogenic effect production. enhancement of susceptibility by treatment of the cells with 5-iododeoxyuridine was evaluated in parallel with untreated cells. cytopathogenic effect was produced in two or more cell lines by every virus tested except six strains of group a coxsackie virus. no cell line was found to be susceptible to these six virus types. i ...	1982	6282217
pancreatic isleitis with coxsackie virus b5 infection.	coxsackie group b virus infection may be responsible for some cases of insulin-dependent diabetes mellitus. however, coxsackie b5 virus is rarely implicated in this respect. the authors observed striking pancreatic islet cell damage in an infant who died of a coxsackie b5 virus infection. the inflammatory response consisted of mononuclear cells, and the acinar tissue was completely uninvolved.	1982	6282732
increase in the saturation of c18 fatty acids induced by coxsackie b6 virus in vero cells.		1982	6285601
cv-1 cells release proteins that facilitate infection by simian virus 40 and echovirus 6.	uninfected simian cells (cv-1) produce two different proteins, one of which enhances the infectivity of echovirus 6 and the other enhances the infectivity of sv40 in less susceptible cells. the enhancers are released by metabolizing cells into the culture medium. the sv40 enhancer protein is larger and less acidic than the echovirus enhancer. the sv40 enhancer protein can be dissociated into 2 subunits with apparent molecular weights of 42,000 and 24,000. the echovirus enhancer protein consists ...	1982	6285865
various phenotypes of diabetes mellitus at ultimate outcome of acutely developed diabetic state induced by viral infection.	for 4 to 8 years we followed up 3 diabetic patients in whom the onset of diabetes seemed to be closely related to the well-documented epstein-barr virus infection (case 1) or coxsackie b4 virus infection (case 2, 3). although all developed acute ketosis-prone diabetes in the convalescent stage of the viral infections, the subsequent clinical courses were quite different from each other. case 1 has remained consistently insulin-dependent and associated with positive islet cell antibody, gastric p ...	1982	6286296
coxsackievirus b infection in pregnant mice and transplacental infection of the fetus.	direct instillation of coxsackievirus b1 into the gastrointestinal tracts of albino mice caused viremia in more than 85% of the animals within 1 day. in pregnant mice infected early in gestation (7 days), the geometric mean titer of virus in the blood was lower (p = 0.02) and the duration of viremia was shorter (p = 0.07) than in nonpregnant female mice, but infection of the heart, liver, and uterus did not differ on each of 5 days after infection. although transplacental infection of the placen ...	1982	6286490
dietary hepatic cholesterol elevation: effects on coxsackievirus b infection and inflammation.	mice made hypercholesterolemic (hc) by diet are highly susceptible to coxsackievirus (cv) b5, whereas normal adult animals remain resistant. in attempting to define those dietary-induced physiological changes which contribute to altered resistance, a strong association between accumulation of intrahepatic cholesterol and increased cv b5-induced mortality was demonstrated, with maximum susceptibility to cv coinciding with a 2.5-fold increase in the ratio of hepatic cholesterol to protein. this me ...	1982	6286492
the virus susceptibility of skin-derived fibroblasts from families with insulin-dependent diabetes mellitus.	we have studied the growth of eight different viruses on skin fibroblasts from three families each having one or more diabetic members and appropriate controls. the haplotypes of all of the family members had been previously characterized. in addition, we have investigated the growth of mumps virus on the lymphoblast cultures from four families of the same type. our results show no difference between the growth of these viruses in cells derived from juvenile diabetics and cells derived from nond ...	1982	6286862
antimicrobial effect of human serum iga.	serum iga, igg and colostrum secretory iga prepared from specimens pooled from a large number of human beings were shown to have measurable levels of antibodies against escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae, poliovirus, coxsackie b virus, echovirus and influenza virus. serum iga exerted a bacteriostatic effect in vitro on e. coli and p. aeruginosa, which increased in the presence of the iron-binding proteins lactoferrin and transferrin. this bacteriostasis was reduced w ...	1982	6287178
diagnostic relevance of humoral and cell-mediated immune reactions in patients with acute viral myocarditis.	sera of 177 patients with acute myocarditis (10 coxsackie b 3/4, four influenza, four mumps, 15 cytomegalovirus, 144 undefined) were tested by indirect immunofluorescence for autoantibodies against heart and skeletal muscle and vital or air-dried adult cardiocytes. antibody-dependent cytolysis, lymphocytotoxicity and antibody-dependent cellular lymphocytotoxicity were assessed using viral adult rat cardiocytes as target cells. muscle-specific anti-sarcolemmal antibodies of the anti-myolemmal typ ...	1982	6288291
role of antiviral antibodies in resistance against coxsackievirus b3 infection: interaction between preexisting antibodies and an interferon inducer.	an experimental model of coxsackievirus b3 infection in newborn mice was utilized to examine the protective role of antiviral antibodies and an interferon inducer, polyinosinic acid-polycytidylic acid [poly(i:c)]. subcutaneous administration to the infected mice of specific antiviral antibodies resulted in significant protection against coxsackievirus b3 infection. antibody-treated animals had shortened viremia, early clearance of virus from tissues, and a reduced mortality rate. dose response t ...	1982	6288570
[polyradiculoneuritis with concomitant arrhythmia caused by coxsackie b virus].		1982	6289535
[virus-induced changes in heart and skeletal muscle].		1981	6289544
development of a shaker culture of buffalo green monkey kidney cells: potential use for detection of enteroviruses.	buffalo green monkey kidney cells were adapted to grow as shaker cultures. replication of environmental and clinical isolates of poliovirus, coxsackievirus, and echovirus in these cultures was analyzed by plaque assay and compared with replication in buffalo green monkey kidney cell monolayers and hep-2 cell shaker cultures. dose-response tests with various concentrations of mahoney type 1 poliovirus indicated that buffalo green monkey kidney cell shaker cultures could detect as little as 1 pfu ...	1982	6289745
[laboratory contribution in the etiologic definition of viral pericarditis].		1982	6290946
impairment of immunocompetent mouse spleen cell functions by infection with coxsackievirus b3.	early after infection, mice inoculated with coxsackievirus b3 showed consistent reduction of antibody responsiveness. beginning one week after infection they also evidenced progressive spleen atrophy. the cellular basis of the reduced antibody response exhibited in vitro by spleen cells of infected mice at the onset of atrophy was investigated by the use of different antigens, by supplementation with different subpopulations of immunocompetent cells from normal donors, and by cross-recombination ...	1982	6292311
acute polyarthritis associated with active epstein-barr virus infection.	nine patients with an initial onset of symptoms of acute arthritis within the preceding four weeks were enrolled in a prospective serological study with clinical follow-up for six months to two years. four adults with chronic rheumatoid arthritis and ten healthy adults were similarly studied. serial titers measured included antibodies to epstein-barr virus (ebv) antigens, group b coxsackieviruses, rubella virus, cytomegalovirus, and herpes simplex virus. serological evidence of active ebv infect ...	1982	6292536
peripheral facial palsy and infections- findings and problems.	eighty-four patients of the cologne university ent clinic with a diagnosis of idiopathic peripheral facial palsy (pep) were examined - both clinically and virologically. in addition, examinations were carried out on 33 further pfp-patients from different practising physicians (group b) where the clinical information, however, was much less detailed. in the ent clinical group (84 patients), there was a total of 12 recent virus infections (9 varicella zoster virus, 2 herpes simplex virus, 1 coxsac ...	1982	6292678
preliminary data on the survival length of enteroviruses in nonalcoholic beverages. inactivating action of some medicinal herb infusions.	vaccinal poliovirus type 1, coxsackie b3 virus and echovirus type 1 survived in mineral water, camomile infusion and coffee for over 20 days. in contrast, chinese tea, lime and mint infusions had a possibly transient inactivating effect on the same enterovirus strains. there was a direct relationship between the concentration of the infusions and their inactivating action. the vaccinal poliovirus was more sensitive than coxsackie and echoviruses to the inactivating action of the infusions.	1982	6293171
[possible localization of the gene of sensitivity of human cells to coxsackie b virus in the short arm of chromosome 21].		1982	6293606
neuraminidase-sensitive erythrocyte receptor for enterovirus type 70.	enterovirus type 70 (ev70) agglutinated human 'o' erythrocytes at 4 degrees c as well as 22 degrees c, but visible agglutination was lost when warmed at 37 degrees c although the virus remained attached to the surface of the erythrocyte. the receptor sites for the virus were neuraminidase-sensitive. a direct involvement of sialic acid on the cell surface in virus-cell interaction was confirmed by the fact that the presence of fetuin or free n-acetylneuraminic acid inhibited the haemagglutinating ...	1982	6294226
isolation of indigenous enteroviruses from chemically treated and dewatered sludge samples.	samples of wastewater sludge were examined for infectious enteroviruses before and after they had been chemically conditioned and dewatered. the least virus was recovered from the cake produced by filter pressing of sludge, which had a greatly increased solids content (39 to 45% [wt/vol]) relative to the untreated sludge (4.2 to 6.2% [wt/vol]) and in one plant was at ph 11 due to the lime conditioner used. conditioning with a cationic polyelectrolyte before dewatering by centrifugation produced ...	1982	6295275
coxsackievirus b4 nephritis in the squirrel monkey.	seventeen squirrel monkeys (saimiri sciureus) were experimentally infected with coxsackievirus b4, and the kidneys, as well as other organs, were studied for pathological changes induced by the virus. seven (41%) of these monkeys developed renal lesions--interstitial and glomerular. the coxsackievirus was identified in 4 of these 7 monkeys (by isolation from the renal tissue in 2, by immunofluorescence staining of viral antigen in 1, and by electron microscopic finding of viral particles in 1). ...	1982	6295434
alterations in coxsackievirus b4 heart muscle disease in icr swiss mice by anti-thymocyte serum.	coxsackievirus b4 (cb4) infection in infant icr swiss mice induces synchronous peaks in both virus titres and pathologic changes in the heart. among surviving mice, transmural necrosis is followed by fibrosis and ventricular aneurysm. rabbit antimouse thymocyte serum (ats) was given before cb4 infection to determine the effects of thymus-dependent functions upon the course of disease. the mortality in ats-treated mice was 75.9% (65 of 83 mice died) compared to 21.3% (16 of 75 mice died) in norma ...	1983	6296300
coxsackie b virus infection in acute myocardial infarction and adult heart disease.	over a 12-month period, 329 patients admitted to the coronary care unit, wellington hospital, new zealand, were studied for evidence of coxsackie b virus infection. fifteen patients (9.8%) with acute myocardial infarction (ami), three (6.5%) with arrhythmia, and three (25%) with heart failure had serological evidence of coxsackie b virus infection. during the same period, two control groups were also studied, and the rates of coxsackie b infection in these groups were compared with that of patie ...	1982	6296646
[patterns in the spread and serovirological diagnosis of serous meningitis].	the epidemiological and clinico-etiological study of cases of acute serous meningitis with unknown etiology in children was carried out in a large industrial city at the period of a considerable morbidity rise caused by this infection. the maximum morbidity was registered among younger children under school age attending children's institutions. in 26 closed groups of children group morbidity was revealed (4 cases and more), in 5 such groups small local outbreaks were registered. the clinico-ins ...	1982	6297190
experimental coxsackie b viral myocarditis in cynomolgus monkeys.	in 11 of 13 cynomolgus monkeys inoculated with coxsackievirus b3 and/or b4, myocarditis was proved histologically. myocarditis was evident the first 10 days after inoculation and left chronic sequelae; moderate myocardial cellular hypertrophy with an increase of connective tissue with focal distribution, and some residual inflammatory foci were found 5 months after viral inoculation. virus was recovered from the heart on the 4th day but not on the 10th day, while serum neutralizing antibody rose ...	1983	6298479
a survey of enteric viruses in domestic sewage.	in this second study (1979-1981) of the viral content of sewage we have demonstrated the presence of poliovirus types 1, 2, and 3 in laval and montreal. several strains of poliovirus types 2 and 3 were nonvaccinal. this is in contrast with our first study (1977-1978) in which only type 1 poliovirus isolates were nonvaccinal. coxsackievirus types b-3, b-4, and b-5 and echovirus types 1, 7, and 11 were also isolated from sewage. interestingly, these isolations coincided with reports of isolation o ...	1983	6299493
temperature-sensitive mutant of coxsackievirus b3 establishes resistance in neonatal mice that protects them during adolescence against coxsackievirus b3-induced myocarditis.	inoculation of neonatal cd-1 mice by multiple routes with an amyocarditic temperature-sensitive (ts) mutant (ts 1) derived from a myocarditic parent variant of coxsackievirus b3 (cvb3(m)) resulted in approximately half of the neonates surviving to adolescence. challenge of the ts 1 survivors with cvb3(m) did not induce myocarditis, as assessed by histological examination of heart tissues. virus was not detected in heart tissues of adolescent ts 1 survivors, but inoculation of these mice with cvb ...	1983	6299950
diversity within a human isolate of coxsackie b4: relationship to viral-induced diabetes.	the ability of different strains of a single virus type to produce different pathogenic expressions is well documented within the picornavirus group. coxsackievirus, group b, type 4 (cb4) has been associated with viral-induced diabetes in man, but expression of its potential to induce diabetes in experimental animals is variable. evidence is presented here for one of the primary sources of this variability that could explain resulting contradictory reports offered in support or rejection of its ...	1983	6300315
isolation and characterization of a membrane-bound population of group b coxsackieviruses.	hela cells infected with several group b coxsackieviruses contain a previously undetected, virus-specific ribonucleoprotein particle which we designated membrane-bound virion (mbv). mbvs of b5 virus have a pronounced polygonal appearance and are slightly smaller than virions. the particles sediment more slowly (at about 107s) and have a lower buoyant density (about 1.30). they contain 35s virion rna; only three, and not four, capsid proteins; and at least seven additional proteins with apparent ...	1983	6300437
preliminary characterization of coxsackievirus b3 temperature-sensitive mutants.	prototype temperature-sensitive (ts) mutants of a coxsackievirus b3 parent virus capable of replication to similar levels at 34 or 39.5 degrees c were examined for the nature of the temperature-sensitive event restricting replication in hela cells at 39.5 degrees c. the ts mutant prototypes represented three different non-overlapping complementation groups. the ts1 mutant (complementation group iii) synthesized less than 1% of the infectious genomic rna synthesized by the coxsackievirus b3 paren ...	1983	6300445
patterns of interaction of effects of light metabolically inert gases with those of hydrostatic pressure as such--a review.	this review of available literature attempts to interpret net effects of metabolically inert light gases (he, h2, and ne) as the resultant of hydrostatic pressure and intrinsic pharmacological effects associated with exposure to these gases, and to assess the relative importance of each component with respect to a number of biological responses. a common pattern is recognizable for pressure reversal of anesthesia, high pressure convulsions, high pressure bradycardia, and certain characteristics ...	1982	6301124
differences in cytolytic t cell response of balb/c mice infected with myocarditic and non-myocarditic strains of coxsackievirus group b, type 3.	two strains of coxsackie b-3 virus, indistinguishable by neutralization with acute mouse antiserum to coxsackie b-3 group virus, differ markedly in pathogenicity. one strain induces extensive mononuclear cell infiltration and necrosis in the heart, and 92% of the infected animals die by day 10 after infection. inflammation, cardiac damage, and mortality are reduced in mice infected with the nonmyocarditic virus. peak virus replication occurs on day 3 with both viruses. virus concentrations decre ...	1983	6301994
coxsackie b4 virus causing insulin dependent diabetes mellitus, myopericarditis and encephalitis--a case report.		1982	6302065
viruses and diabetes mellitus.		1982	6302066
coxsackie b virus infections in new zealand patients with cardiac and non-cardiac diseases.	from 1979 to 1981, a total of 910 cardiac patients and 776 noncardiac patients were studied and compared for evidence of coxsackie b virus infections. of 78 cardiac patients with coxsackie b infection, 30 had pleurodynia, 18 myocarditis, and 20 pericarditis. the age-adjusted rates of infection per 100 cases of defined category were 7.2, 13.3, and 7.0, respectively. of 69 noncardiac patients with coxsackie b infection, 27 presented with pyrexia of unknown origin, 14 with upper respiratory tract i ...	1983	6302218
a study of school children who had identified virus infections of the central nervous system during infancy.	forty-nine children who had a virus infection of the central nervous system (cns) when under 1 year of age were studied. one child had died during the initial illness and three of the survivors were severely disabled. the other survivors, more than 5 years after the initial illness, were all attending normal schools. these 45 children, together with 45 matched controls, were examined. we confirm the findings of other studies that virus infections of the cns in infancy may cause severe disabiliti ...	1983	6303619
coxsackie b virus infection in coronary care unit patients.	it has been suggested that coxsackie b virus infections may play a part in causing or triggering myocardial infarction. this study was designed to compare the incidence of such infections in coronary care unit patients and normal controls. the choice of a suitable criterion for diagnosis of coxsackie infection is discussed fully. two hundred and fifty admissions to a coronary care unit and 100 control subjects had a serum sample tested by microneutralisation for coxsackie b antibodies. the incid ...	1983	6304150
human skin fibroblasts are nonpermissive to coxsackie b4 infection: an age-dependent phenomenon.	human skin fibroblasts were previously shown to be resistant to coxsackie b4 virus infection. we have cultured fibroblasts from skin and lung tissues of donors of various ages. by a novel method for direct assay of virus absorption and penetration, we have shown that skin fibroblasts from young fetuses are susceptible to coxsackie b4 infection, whereas those from older fetuses, children and adults are not and that this refractoriness is caused by a tissue-specific block to virus penetration.	1983	6305870
tests for coxsackie b virus-specific igm.		1983	6306096
coxsackie b infection in a general medical unit.	from january 1979 until december 1980, neutralizing antibody titres to coxsackie b1-6 viruses were measured in sera from 220 patients presenting to a general medical unit, the majority of whom had chest pain. no four-fold or greater rises in antibody titre were detected in these hospital patients. however, 49 per cent had titres of greater than or equal to 256 to one or more coxsackie b virus types compared with 10 per cent of the 950 persons studied during a 'normal population' survey in the we ...	1983	6306762
cellular immune mechanisms in coxsackievirus group b, type 3 induced myocarditis in balb/c mice.	coxsackie b viruses are a common cause of viral myocarditis in humans. a murine model of the human disease has been developed using coxsackievirus group b, type 3 and inbred balb/c mice. infection of t lymphocyte deficient mice does not result in significant myocarditis indicating the importance of t cells in this disease. the virus can be isolated from the hearts of t cell deficient and normal mice in equal concentrations. virus elimination presumably is mediated by virus specific neutralizing ...	1983	6307010
two cases of coxsackie b2 infection in neonates: clinical, virological, and epidemiological aspects.	two cases of neonatal coxsackie virus b2 infection are described. one infant presented with meningitis and enteritis, the other with rhinitis, meningoencephalitis, and enteritis. both infants made good recoveries. the virus infection could also be demonstrated in all nonimmune family members, most of whom gave a history of recent mild febrile disease (pharyngitis, diarrhea). enterovirus infections may be suspected in cases of neonatal meningitis or myocarditis associated with gastrointestinal si ...	1983	6307701
eclipse of coxsackievirus infectivity: the restrictive event for a non-fusing myogenic cell line.	coxsackieviruses a2, a5 and b3 did not replicate in l8cl3-u cells (a non-fusing variant of the rat l8 myogenic cell line) although these cells possessed a common receptor for coxsackieviruses a2 and a5, and a different receptor for coxsackievirus b3. the restriction in replication was identified as a block in viral eclipse, since 6 m-licl treatment permitted recovery of the coxsackievirus a2 inoculum from l8cl3-u cells after 2 h at 37 degrees c, and the cells could be transfected by viral rna. c ...	1983	6308134
variation in virulence of coxsackie virus b3 in the hearts of mice. i. comparison of mortality and virus growth in the heart and other organs.	virulence of coxsackie virus b3 (cb3) was compared in mice between strain sk-74 isolated from a patient and strain t-70 isolated from a healthy child as well as between prototype strain nancy and its mouse-passaged derivative strain pmh. strain sk-74 showed a high mortality (40-80%), while strain t-70 did not induce deaths in mice (mortality: 0%). strain pmh showed a high mortality (65-85%), but strain nancy did not cause deaths in the mice. in agreement with the mortality trend, virus titers in ...	1983	6308400
[epidemic chest pain caused by coxsackie virus b3].		1982	6309400
levamisole exacerbates coxsackievirus b3-induced murine myocarditis.	levamisole administration to several strains of adolescent mice at the time of or up to 4 days post-inoculation (p.i.) with a myocarditic variant of coxsackievirus b3 (cvb3m) increased the number of myocarditic lesions above that found in cvb3m-inoculated mice. virus replication in heart tissues in vivo was not affected by levamisole administration to the mice, nor was production of neutralizing antibody to cvb3m. lymphocytes from nodes of virus-inoculated mice treated with levamisole at 2 days ...	1983	6309663
experimental coxackievirus b 1 infection in immunologically altered mice.	the patterns of response to coxackievirus b1 infection were studied in intragastrically infected mice differing with respect to their state of immunity. it is shown that coxsackie-viral infection of mice with lymphocytopenia elicited by pretreatment with lymphocyte-specific antiserum resulted in higher virus titres, longer virus persistence and more accentuated histologic changes in the parenchyma of target organs than seen in a control group of infected mice. on the other hand, in mice infected ...	1983	6309957
viral content of a sewage-polluted intertidal zone.		1980	6309967
myalgic encephalomyelitis--report of an epidemic.	the relationship between the group b coxsackieviruses and a wide variety of illnesses, particularly pleurodynia and myo/pericarditis, is already well established. the detection of raised levels of neutralizing antibody to these viruses in a group of patients in a rural practice presenting with an illness resembling myalgic encephalomyelitis (me) was therefore unexpected. this is a most distressing and debilitating illness for the patient and the affected family. what is the immunological failure ...	1983	6310104
variation in virulence of coxsackie virus b3 in the heart of mice, ii. pathological comparison.	histopathological analysis of the heart in adult mice inoculated with coxsackie virus b3 (cb3) strains revealed that strain sk-74 isolated from a patient suffering from severe diarrhea and fever produced severe myocarditis but strain t-70 isolated from a healthy child induced no lesion in the hearts of mice tested, and that intensities of myocardial lesions in mice inoculated with strain pmh were higher than those in mice inoculated with prototype strain nancy. the results support the conclusion ...	1983	6310354
haemolytic uraemic syndrome: evidence of multiple viral infections in a cluster of ten cases.	during july 1979, ten patients were admitted to the hospital with bloody diarrhoea followed by manifestations of haemolytic uraemic syndrome (hus): acute microangiopathic haemolytic anaemia, intravascular coagulopathy, and impaired renal function. ages ranged from 13 months to 58 yr, with only two patients more than 5 yr old. in a household that included seven children born to three sisters who married three brothers, six children required hospitalization for bloody diarrhoea and four developed ...	1983	6311967
monoclonal antibodies that inhibit attachment of group b coxsackieviruses.	hybridoma cell lines that secrete monoclonal antibodies which react with hela cell surface antigens were produced. the monoclonal antibodies prevented cytopathic effects caused by coxsackievirus b1 and significantly reduced the amounts of coxsackieviruses b1, b5, and b6 that absorb to hela cells. these antibodies did not protect the cells from poliovirus cytopathic effects, and they had no effect on the attachment of other picornaviruses to hela cells.	1983	6312109
cross antigenicity among enteroviruses as revealed by immunoblot technique.	antigenic relationships of various human and two animal picornaviruses were investigated by the immunoblotting ("western blot") technique. the viruses included all coxsackievirus b types (1-6), poliovirus types 1-3, several strains of echovirus 11, emc virus, and fmdv. antisera included human sera and sera from rabbits hyperimmunized with either purified picornaviruses, viral structural polypeptides (vp8), boiled or "sample-boiled" virions. group-specific reactions of various extent were observe ...	1983	6312682
coxsackie b virus antibodies in myocardial infarction.	evidence for the association between coxsackie b virus infections and myocardial infarction was studied in a prospective follow-up examination. using the micro neutralization test, 9 (15%) of 59 patients with acute myocardial infarction and 1 (2.6%) of 38 control patients showed a fourfold, or higher, antibody increase in paired serum samples against coxsackie b1-5 viruses. this difference is significant (p less than or equal to 0.05). none of the patients or controls revealed symptoms of a vira ...	1983	6312770
neutralizing antibodies against coxsackie b viruses in patients with recent onset of type i diabetes.	in a search for coxsackie b virus-induced, insulin-dependent diabetes mellitus (iddm) we examined sera from 166 selected patients (age 1-17 years) with recent onset of iddm for specific neutralizing antibodies. all 166 patients investigated had a clinical history of recent infectious illness. eighty per cent of the patients had antibodies against at least one coxsackie b virus type. but even among the children studied with antibody titers higher than 256 only in about 44% could a recent coxsacki ...	1983	6313373
the development of type 1 (insulin-dependent) diabetes mellitus: two contrasting presentations.	genetic, immunological and viral factors have been implicated in pathogenesis of type 1 diabetes mellitus. the development of type 1 diabetes in two siblings of patients with type 1 diabetes studied as part of a large epidemiological study, is described. one case, a 13-year-old male not sharing either hla haplotype with his diabetic sister, had virtually normal glucose tolerance 80 days before symptomatic presentation. he showed serological evidence of infection by coxsackie cb4 (at diagnosis) a ...	1983	6313459
the role of t lymphocytes in the pathogenesis of coxsackie virus b3 heart disease.	myocarditis in athymic balb/c-nu/nu (nu/nu) mice infected with coxsackie virus b3 was studied to determine whether inflammatory mononuclear cell infiltration was produced by transfer of spleen cells ob balb/c-nu/+ (nu/+) mice infected with the same virus. in addition, spleen cells of uninfected nu/+mice were transferred into athymic nu/nu mice infected with coxsackie virus b3. athymic nu/nu mice infected with coxsackie virus b3 after transfer of spleen cells of nu/+ mice infected with the same v ...	1983	6315043
a long-term follow-up study of acute viral and idiopathic myocarditis.	in order to clarify the prognosis of myocarditis and the relationship between myocarditis and idiopathic cardiomyopathy, 20 patients with myocarditis (one with coxsackie b; one with rubella and 18 with idiopathic myocarditis) were followed up for a long period using echocardiography and holter electrocardiographic monitoring. the follow-up period was 49.1 +/- 39.3 months (mean +/- sd). subjects were classified into the following 4 groups according to their prognoses, left ventricular end-diastol ...	1983	6315985
[differential diagnosis of myocarditis, cardiomyopathy and chronic ischemic heart disease].	a total of 108 patients with myocarditis and cardiomyopathies and 25 with chronic coronary disease (ccd) were investigated. the most informative diagnostic criteria were identified for the differentiation between noncoronarogenic myocardial disease (nmd) and ccd. bicycle ergometry was positive in all ccd patients, whereas in those with nmd it was negative or had to be discontinued because of fatigue. nmd was associated with increased activity of transaminases, ldh and its isonenzymes (first and ...	1983	6316018
the role of immunoglobulins in amniotic fluid growth inhibition of bacteroides fragilis, herpes simplex, coxsackie b 5 and cytomegalovirus.	in serum and amniotic fluid (af) from 30 pregnant women, antibodies against the anaerobe bacteroides fragilis were determined by enzyme-linked immunosorbent assay (elisa) and the results were compared with those of previously performed tests of b. fragilis growth inhibition by af. the neutralizing effects of serum and af against herpes simplex virus (hsv), cytomegalovirus (cmv) and coxsackie b 5 virus were also investigated, and antibody titres were determined by elisa. no correlation could be d ...	1983	6316121
viruses in the pathogenesis of type i diabetes.		1983	6317287
myocarditis in siblings leading to chronic heart failure.	two brothers, aged 7 and 9, presented 4 years apart with progressive heart failure following a probable viral infection. electrocardiograms of both showed widespread precordial q waves. cardiac catheterization in each case revealed almost equal right atrial, right ventricular and pulmonary artery pressures and poorer contraction of the right than left ventricle. high neutralizing antibody titres to coxsackie b4 virus were found in the siblings and their mother. widespread post-myocarditic scarri ...	1983	6317392
antidiabetic treatment and coxsackie virus in diabetes.		1983	6317551
ventricular aneurysms and ventricular arrhythmias complicating coxsackie virus b1 myocarditis of syrian golden hamsters.	we inoculated coxsackie virus b1 (cvb1) intraperitoneally into 36 2-week-old syrian golden hamsters. serial ecgs were recorded from 10 hamsters which survived the acute and electrocardiographically manifest myocarditis. in a mean 17.8 week (range 9 to 31 weeks) follow-up period, chronic ventricular premature contractions (vpc) followed the acute ecg changes in three of the animals. vpc were recorded in a uniform contour in a hamsters, which had a ventricular aneurysm and were of two kinds of con ...	1984	6318997
in vitro culture of coxsackievirus group b, type 3 immune spleen cells on infected endothelial cells and biological activity of the cultured cells in vivo.	spleen cells from male balb/c mice infected 7 days earlier by an intraperitoneal injection of 3 x 10(4) pfu of a myocarditic strain of coxsackievirus b-3 lysed virus-infected endothelial cells in a 51cr release assay. cytotoxic activity in the in vivo sensitized spleen cell population could be further increased by culturing the immune spleen cells from infected mice on virus-infected or uninfected endothelial cells for 6 to 7 days in vitro. cytotoxicity of in vitro cultured spleen cells to infec ...	1984	6319285
some long-term sequelae of coxsackie b virus infection.		1984	6319692
altered receptor specificity of coxsackievirus b3 after growth in rhabdomyosarcoma cells.	serial "blind" passages in human rhabdomyosarcoma (rd) cells of prototype viruses from each of the six immunotypes of the group b coxsackieviruses (cb) resulted in the isolation of intratypic variants of cb1, cb3, cb5, and cb6. each variant virus strain acquired the capacity to agglutinate human erythrocytes and produce small plaques on hela cells, although their serological specificity remained unchanged. an alteration in vp1 mobility in sodium dodecyl sulfate-polyacrylamide gel electrophoresis ...	1984	6321753
[risk of intrauterine infection of the human fetus with coxsackie viruses in pregnancy complicated by late toxicosis and pyelonephritis].	during virological examinations of 144 pregnant women including 85 women with complicated course of pregnancy coxsackie a viruses were isolated from the blood or identified in the cells of urinary tracts in 60.3% of the cases of nephropathy, in 68.3% of the pregnant women with pyelonephritis, and in 8.5% of normal subjects. the groups of the pregnant women under comparison did not differ significantly in the rate of finding of other viruses. coxsackie a viruses were also isolated from the placen ...	1983	6322456
a broad-spectrum enzyme-linked immunosorbent assay for the detection of human enteric viruses.	an enzyme-linked immunosorbent assay (elisa) test has been devised for detection of a broad spectrum of human enteric viruses, based on the use of poly-l-lysine as a nonspecific adhesive to hold the virus particles in the test wells and of pooled human immune serum globulin to mark the virus for detection with commercial goat anti-human igg antibody conjugated with horseradish peroxidase. detection of five human enteroviruses and a reovirus at levels of 10 most probable number of cytopathogenic ...	1984	6323511
heterotypic reactions in a radioimmunoassay for coxsackie b virus specific igm.	igm antibody capture radioimmunoassays were developed to detect coxsackie virus b1-b5 specific igm. specific igm was detected in sera from all patients with coxsackie b virus infections proved by isolation; however, sera from 13/32 patients with rising neutralising antibody titres were negative in the assay. frequent heterotypic responses were seen among the positive sera. thirty seven patients with other enterovirus infections were also studied, and sera from 15 of these patients reacted in the ...	1984	6323548
invasiveness of salmonella typhimurium in hep-2 cell cultures preinfected with coxsackie b 1 virus.	the influence of infection of hep-2 cells with coxsackie b 1 virus on the invasiveness of salmonella typhimurium has been studied. the bacterial invasiveness was significantly increased in the cell cultures incubated with virus before bacterial inoculation. this effect was a function of time after introduction of virus into the cell cultures and the concentration of virus. the increase in bacterial invasiveness was observed before production of infectious virus particles and before development o ...	1984	6324532
a murine model of polymyositis induced by coxsackievirus b1 (tucson strain).	a murine model of polymyositis induced by coxsackievirus b1, tucson strain (cvbt) is described. intraperitoneal cvbt inoculation of cd 1 swiss mice less than 48 hours old resulted in proximal hindquarter weakness that was first apparent 7 days after viral challenge and persisted for more than 10 weeks. electromyographic and histologic evidence of a continuing myositis was present during this entire period of time. however, virus was not detectable later than 2 weeks post infection, despite clini ...	1984	6324823
acute tonsillitis in children: microbial pathogens in relation to age.	tonsil swabs were collected from 248 children (1 through 10 yr) who presented with acute tonsillitis. a beta-hemolytic streptococcus was grown from 129 (53%) of 244 cases and as the sole pathogen from 83 (47%) of 178 cases. beta-hemolytic streptococci were especially common over the age of 6 yr (68% of children) relatively uncommon under the age of 4 yr (18%); most (90%) streptococci belonged to group a; 18 streptococcal t types were represented amongst 88 group a strains; the potentially nephri ...	1984	6326030
[changes in antibody titers against an enterovirus (echo 22) in north bavaria in relation to mortality from heart disease 1977 to 1981].	in upper and middle franconia, two districts of northern bavaria, with a population of 2.5 millions, an increase of deaths caused by heart diseases according to code number 420-429 of the "international classification of diseases" has been ascertained by evaluation of data from the bavarian state office for statistics and data processing. figures rose from about 2000 cases in 1977 to more than 3000 in 1979. sera from patients from these neighbouring regions (about 600 per year) have been examine ...	1984	6326426
enterovirus types in samples of activated sewage sludge.	samples of activated sewage sludge from two different wastewater treatment plants in lorraine were collected in january and february 1983. after elution and concentration, virus contents were estimated with the under agar technique, utilizing bgm cells. a total of 436 pfu was counted, and after viral confirmation, 14% (61 isolates) were typed with the serum neutralization test, using the lim benyesh-melnick pools a-o. most frequently polioviruses (41%) were found, followed by coxsackie viruses g ...	1984	6326427
differing cardiotropic and myocarditic properties of group b type 4 coxsackievirus strains.	a murine model system for evaluation of myocarditic and cardiotropic properties of strains of group b, type 4 coxsackievirus (cbv-4) was developed in male balb/c mice 4 weeks of age. differing cardiotropic and myocarditic properties could be identified among field strains within the cbv-4 serotype. these properties were consistent for the virus strains, and were independent of the infecting virus dose. virus replication in the heart appeared to be essential for development of myocarditis, but so ...	1984	6326709
[a serological monitoring of coxsackie b virus].		1983	6327042
a longitudinal study of virus antibodies in patients with newly diagnosed type 1 (insulin-dependent) diabetes mellitus.	seventeen newly diagnosed type 1 diabetics were studied longitudinally--at diagnosis, after 5 months and after 2 years, for antibodies against the following viruses: coxsackie b3, b4 and b5, echo 11, and adeno 7a. the latter two were chosen because they had been isolated frequently in the area during the year the patients were diagnosed, whereas the coxsackie viruses previously have been associated with the aetiology of type 1 diabetes. coxsackie b4 antibody titres fell from the diagnosis and th ...	1984	6327989
[arthritis associated with coxsackie b virus infections].		1984	6328634
[familial hemolytic-uremic syndrome associated with coxsackie b infection].	authors describe hemolytic uremic syndrome (hus) in two siblings whose onset occurred within eight days. there was evidence of having recent infection with coxsackie b virus. clinical course, treatment and outcome of patients are reported, showing differences on the severity of renal failure and platelet consumption. recovery of renal function was evidenced in both infants. one of them critically thrombopenic and with oligoanuric renal failure was treated with peritoneal dialysis, aspirin and di ...	1984	6329051
meningo-encephalitis caused by coxsackievirus group b type 2. diagnosis confirmed by measuring intrathecal antibody.	we describe a case of meningo-encephalitis caused by coxsackievirus group b type 2. diagnosis was suggested by isolation of virus from faeces and high titres of serum antibody in which coxsackie virus b2 specific igm antibody was detected. evidence of intrathecal production of antibody confirmed the diagnosis.	1984	6330208
[etiology of diabetes mellitus--is diabetes of a viral origin?].		1984	6330400
[participation of coxsackie b viruses in the occurrence and recurrence of infectious-allergic myocarditis (a serological study)].	the serological study of sera from patients with infectious allergic myocarditis and from healthy persons has revealed essential differences in the occurrence and titers of antibodies to coxsackie virus b. in patients with infectious allergic myocarditis the infectious process has been found to be significantly more frequently linked with coxsackie viruses b, serovars 2 and 4.	1984	6331031
coxsackievirus b-3 myocarditis in balb/c mice. evidence for autoimmunity to myocyte antigens.	male balb/c mice inoculated with a heart-adapted variant of coxsackievirus, group b, type 3 (nancy) (cvb3m), develop extensive myocarditis and cytolytic activity to primary cultures of uninfected and infected myocytes. to elucidate the mechanisms of myocyte injury in myocarditis, two distinct cytolytic t-lymphocyte (ctl) populations were isolated by immunoadsorption of lymph node cells to glutaraldehyde-fixed uninfected and infected myocyte monolayers. one population preferentially adsorbed to a ...	1984	6331168
virus transport and survival after land application of sewage sludge.	the survival and transport patterns of poliovirus 1 and echovirus 1 were studied in undisturbed soil cores which were treated with digested sludge and exposed to natural weather conditions prevailing in north central florida. it was shown that, under those experimental conditions, enteroviruses are relatively rapidly inactivated in the soil. a more rapid virus decline was observed during the warm and dry fall season than during the warm and wet summer season. the monitoring of soil core leachate ...	1984	6331308
removal of enteric viruses from surface water at eight waterworks in the netherlands.	eight waterworks in the netherlands, which use surface water as their raw water source, were sampled repeatedly between november 1978 and june 1981. at five waterworks , 30 of 45 samples of raw water contained viruses. of 55 samples of partially purified water, 11 were virus positive, including 8 after coagulation, sedimentation, and rapid sand filtration, 2 after storage, coagulation, sedimentation, transport chlorination, and rapid sand filtration, and 1 after storage in open reservoirs for 5 ...	1984	6331309
influence of water quality on enteric virus concentration by microporous filter methods.	four enteric viruses, poliovirus type 1, echovirus type 1, reovirus type 3, and simian adenovirus sv-11, were concentrated from seeded 1.3-liter volumes of raw, finished, and granular activated carbon-treated waters by adsorption to 47-mm-diameter (17 cm2), electropositive ( virosorb 1mds ) filters at ph 7.5 or electronegative ( filterite ) filters at ph 3.5 with and without 5 mm added mgcl2, followed by elution with 0.3% beef extract in 50 mm glycine at ph 9.5. removal of particulates from raw ...	1984	6331310
simultaneous concentration of four enteroviruses from tap, waste, and natural waters.	the efficiency of virus recovery from water was investigated by using a method which enabled the concentration of a mixture of four enteroviruses with determination of their individual recovery efficiencies. the four viruses used (poliovirus 1, coxsackievirus a9, coxsackievirus b1, and echovirus 7) represented each of the four major subgroups of enteroviruses. this method, which was based on selective antibody neutralization, was used to investigate the effects of input water quality on enterovi ...	1984	6331314
persistent infection of mouse fibroblasts with coxsackievirus.	infection of fibroblast cell lines initiated from balb/c or nfr mice with coxsackievirus b3 (cbv-3) or b4 (cbv-4) resulted in infections which persisted for a limited number of subpassages of the infected cells in most cases, but for over a year in one case. in all instances primary acute infections were characterized by cytopathology and release of infectious virus progeny. viral antigen could be detected during the acute phase of infection, but not in subcultured infected cells. infectious cen ...	1984	6331352
immunoglobulin responses to echovirus type 11 by enzyme linked immunosorbent assay: single-serum diagnosis of acute infection by specific igm antibody.	an indirect solid-phase enzyme-linked immunosorbent assay (elisa) for the determination of specific igm and igg antibodies to echovirus type 11 in a single dilution of serum was developed using partially purified echovirus type 11 bound to microplates. whole serum was used for igg antibody but prior to assaying for igm antibody interfering igg was removed by ion exchange chromatography. the elisa for echovirus type 11 igg antibody was a more sensitive, rapid, technically easier and less costly a ...	1984	6394604
[changes in the membrane fluidity of human neutrophilic granulocytes under the effect of a chemoattractant (fmlp) and of echo virus, type 9, a. barty strain].	preincubation of human polymorphonuclear leucocytes (pmns) with different concentrations of echo virus, type 9, strain a. barty results in viral dose- and time-dependent inhibition of chemotactic cellular response to chemoattractant (n-formylmethionylleucylphenylalanine = fmlp). in the present experiments, by means of biophysical methods using excimer forming lipids (pyrendecanoic acid) the influence of fmlp and echo 9 virus on membrane fluidity of pmns was investigated. it is shown that the inc ...	1983	6412297
echo 9 virus-induced order-disorder transition of chemotactic response of human polymorphonuclear leucocytes: phenomenology and molecular biology.	by means of functional, morphological, and biophysical methods the in vitro interaction of echo virus, type 9, strain a. barty with human polymorphonuclear leucocytes (pmns) was investigated and analyzed by statistical methods. control cells and virus-treated pmns (15 min, 37 degrees c; pmn: virus (pfu)-ratio ranging from 1:1 to 1:50) were exposed to a chemotactic gradient (n-formylmethionyl-leucylphenylalanine = f-met-leu-phe, 10(-8) m/mm) in a zigmond chamber. whereas the track velocity of the ...	1984	6487817
studies of echovirus-12 in volunteers: determination of minimal infectious dose and the effect of previous infection on infectious dose.	a two-part study of echovirus-12 was done in volunteers. in the first part the human infectious dose of the virus was determined in 149 healthy adults with undetectable serum antibody, each of whom drank 0-330,000 plaque-forming units (pfu) of virus in 100 ml of nonchlorinated water. infection was defined as fecal shedding of virus or significant (fourfold or greater) increases in serum antibody titer. the hid50 (i.e., the dose required for infection of 50% of the volunteers) was 919 pfu. throug ...	1984	6501929
a case of agammaglobulinaemia complicated by meningoencephalitis due to echo virus 27.	a boy with agammaglobulinaemia diagnosed and treated from the age of 3 years 2 months developed encephalitis at 4 years 3 months. the illness showed a remitting but deteriorating course until death aged 6 years 5 months. echo virus type 27 was isolated from the cerebrospinal fluid during the terminal illness. the virus was not isolated from five earlier cerebrospinal fluid samples nor was virus detected in a temporal lobe biopsy.	1984	6508664
[sensitive virologic evaluation in suspected meningitis: study of a radioimmunotest for the detection of igm antibodies against echo 9 and echo 11 viruses].	the majority of viral meningitis cases is known to be due to echo virus infections on one hand, and mumps on the other. while the latter can be diagnosed by igm antibody detection from one serum sample in the acute stage, diagnosis of enterovirus infections is by virus isolation and typing. an igm-antibody test for echo 9 and 11 viruses is presented to evaluate the possibility of rapid serological diagnosis of echo virus meningitis cases. 36 cases from five local outbreaks due to echo 6, 9, 11, ...	1983	6612271