Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| sex determination in 6 bovid species by duplex pcr. | sex determination in domestic animals is of potential value to livestock breeding programs. the aim of this study was to develop a simple and accurate pcr-based sex determination protocol, which can be applicable to 6 major domesticated species of the family bovidae, viz. bos frontalis, b. grunniens, b. indicus, bubalus bubalis, capra hircus, and ovis aries. in silico analysis was done to identify conserved dna sequence in the hmg box region of the sex-determining region of the y-chromosome (sry ... | 2008 | 19043179 |
| buffalo (bubalus bubalis) pre-antral follicle population and ultrastructural characterization of antral follicle oocyte. | the main objectives of the present study were to determine the ultrastructural modifications occurring in the oocyte during late folliculogenesis and to estimate pre-antral follicle population in buffalo. half the collected ovaries were fixed and prepared for optic microscopy; the antral follicles from the other ovaries were measured and individually punctured. the cumulus-oocyte complexes (cocs) were processed for transmission electron microscopy. the number of pre-antral follicles in buffalo o ... | 2010 | 19055556 |
| effects of tiamulin, neomycin, tetracycline, fluorophenicol, penicillin g, linco-spectin, erythromycin and oxytetracycline on controlling bacterial contaminations of the river buffalo (buballus bubalis) semen. | in order to investigate the effects of tiamulin, neomycin, tetracycline, fluorophenicol, penicillin g, linco-spectin (0.15 mg ml(-1) lincomycin + 0.3 mg ml(-1) spectinomycin), erythromycin and oxytetracycline on controlling bacterial contaminations of the river buffalo semen, 120 ml diluted buffalo bull semen (diluted by tris-egg yolk extender) was divided into 5 ml tubes after initial evaluation and before (control sample) and at 0, 2, 6, 12 and 24 h after adding each of the above antibiotics a ... | 2007 | 19090126 |
| in vitro culture and differentiation of buffalo (bubalus bubalis) spermatogonia. | the objective of this study was to develop a culture system which could support buffalo spermatogonia differentiation into spermatids in vitro. testes from 3- to 5-month-old buffaloes were decapsulated and seminiferous tubules were enzymatically dissociated to recover spermatogonia and sertoli cells. the cells were cultured in modified dulbecco modified eagle medium supplemented with different concentrations of foetal bovine serum, retinol, testosterone for 2 months at 37 degrees c. spermatogoni ... | 2010 | 19090820 |
| induction of ovulation of ovulatory size non-ovulatory follicles and initiation of ovarian cyclicity in summer anoestrous buffalo heifers (bubalus bubalis) using melatonin implants. | this study was conducted on summer anoestrous buffalo heifers to monitor the efficacy of melatonin for induction of ovulation and ovarian cyclicity. during pre-treatment period of 24 days, the ovarian dynamics of five cycling and 10 summer anoestrous heifers was monitored on each alternate day using a transrectal ultrasound scanner. thereafter, during treatment period, these 10 anoestrous heifers along with additional seven anoestrous heifers were randomly allocated into non-implanted (n = 5) an ... | 2010 | 19090824 |
| effect of different combinations of cryoprotectants on in vitro maturation of immature buffalo (bubalus bubalis) oocytes vitrified by straw and open-pulled straw methods. | this study was designed to evaluate effects of different combinations of cryoprotectants on the ability of vitrified immature buffalo oocytes to undergo in vitro maturation. straw and open-pulled straw (ops) methods for vitrification of oocytes at the germinal vesicle stage also were compared. the immature oocytes were harvested from ovaries of slaughtered animals and were divided into three groups: (i) untreated (control); (ii) exposed to cryoprotectant agents (cpas); or (iii) cryopreserved by ... | 2010 | 19090828 |
| cleft palate in a male water buffalo calf. | congenital palatal defects are common in animals but there is only one report of water buffalo has been recorded in iran. one died male water buffalo calf was examined after hysterotomy operation. at necropsy findings, brachygnathia, palate cleft and small lungs were diagnosed. it is the second report of water buffalo cleft palate in iran. | 2007 | 19093537 |
| feed supplementation prevents post-conception decline in milk progesterone concentrations associated with production stress in dairy buffaloes (bubalus bubalis). | the onset of pregnancy may be associated with hormonal changes and decline in milk yield of buffaloes. to investigate this, forty lactating buffaloes from 1st to 23rd weeks post-conception were selected. the animals were assigned to three treatments: prt, prs, nprt and three milk yielding groups hmy, 66 to 75 l/week, n = 12; mmy, 56 to 65 l/week, n = 16; lmy, 46 to 55 l/week, n = 12). milk samples were collected on alternate weeks and analyzed with ultrasonic milk analyzer. eia was used for milk ... | 2009 | 19107569 |
| a first generation whole genome rh map of the river buffalo with comparison to domestic cattle. | the recently constructed river buffalo whole-genome radiation hybrid panel (bburh5000) has already been used to generate preliminary radiation hybrid (rh) maps for several chromosomes, and buffalo-bovine comparative chromosome maps have been constructed. here, we present the first-generation whole genome rh map (wg-rh) of the river buffalo generated from cattle-derived markers. the rh maps aligned to bovine genome sequence assembly btau_4.0, providing valuable comparative mapping information for ... | 2008 | 19108729 |
| transmission dynamics of schistosoma japonicum in the lakes and marshlands of china. | schistosoma japonicum is a major public health concern in china, with over one million people infected and another 40 million living in areas at risk of infection. unlike the disease caused by s. mansoni and s. haematobium, schistosomiasis japonica is a zoonosis, involving a number of different mammalian species as reservoir hosts. as a result of a number of published reports from china, it has long been considered that bovines, particularly water buffaloes, play a major role in human s. japonic ... | 2008 | 19115007 |
| in vitro production of cattlexbuffalo hybrid embryos using cattle oocytes and african buffalo (syncerus caffer caffer) epididymal sperm. | interspecies hybridization of bovids occurs between domestic cattle and at least three other species; american bison (bison bison), yak (bos grunniens) and banteng (bos banteng). birth of a cattlexbuffalo (bubalus bubalis) hybrid has reportedly occurred in russia and in china, but these reports were not authenticated. such hybrids could be important in improving livestock production and management of diseases that impede production in tropical africa. this study investigated hybridization betwee ... | 2009 | 19118889 |
| effect of bupivacaine on epidural analgesia produced by xylazine or medetomidine in buffaloes (bubalus bubalis). | to evaluate and compare the effect of epidural bupivacaine on analgesia produced by epidural xylazine or medetomidine in buffaloes. | 2009 | 19121162 |
| characterization and expression profile of complete functional domain of granulysin/nk-lysin homologue (buffalo-lysin) gene of water buffalo (bubalus bubalis). | granulysin (gnly)/nk-lysin (nkl) is an effector antimicrobial cationic peptide expressed in the cytotoxic and natural killer lymphocytes. we report here cdna sequence (405bp) encoding the complete functional domain of buffalo-lysin (bu-lysin), and its expression profile in the various tissues. the nucleotide sequence of bu-lysin exhibited >85% identity with the bovine lysin. comparison of the deduced amino acid sequence of bu-lysin with those of gnly/nkl of different species revealed the conserv ... | 2009 | 19136155 |
| detection of bovine viral diarrhea virus from three water buffalo fetuses (bubalus bubalis) in southern italy. | bovine viral diarrhea virus (bvdv) is an important pathogen that primarily infects ruminants, leading to several clinical problems including abortion. bvdv-specific antibodies were reported in a wide range of hosts within domestic and wildlife animal populations, and serological studies also indicated bvdv infection in buffaloes. the purpose of this study was to analyze the presence of bvdv in 2 water buffalo (bubalus bubalis) herds with a history of abortion. virus isolation from aborted fetuse ... | 2009 | 19139516 |
| transcriptional analysis of buffalo (bubalus bubalis) oocytes during in vitro maturation using bovine cdna microarray. | the need for improving in vitro production of buffalo embryos necessitates a better understanding of the molecular mechanisms regulating early development including oocyte maturation. here, we used bovine cdna microarray platform to investigate mrna abundance of buffalo oocytes before and after in vitro maturation. for this, a total of six pools each contains 50 immature or in vitro matured buffalo oocytes were used for mrna isolation and subsequent cdna synthesis. the bluechip bovine cdna micro ... | 2010 | 19144006 |
| generation and characterization of embryonic stem-like cell lines derived from in vitro fertilization buffalo (bubalus bubalis) embryos. | in the present study, buffalo embryonic stem-like (es-like) cell lines were successfully isolated, cultured and characterized. from a total of 92 normal buffalo embryos obtained by in vitro fertilization, 18 were morulae, 33 were blastocyst and 41 were hatched blastocyst, the inside of morulae or inner cell masses of blastocysts were isolated mechanically and cultured onto mitomocin-c-inactivated buffalo embryonic fibroblasts as feeder layers. alkaline phosphatase (ap) of es-like cells, as well ... | 2010 | 19144015 |
| generation of buffalo (bubalus bubalis) transgenic chimeric and nuclear transfer embryos using embryonic germ-like cells expressing enhanced green fluorescent protein. | the possibility of producing transgenic buffalo embryos by chimera and nuclear transfer (nt) using buffalo embryonic germ (eg)-like cells expressing enhanced green fluorescent protein (egfp) has been explored in this study. buffalo eg-like cells and fibroblasts with two to eight passages were transfected with the lined plasmid (pce-egfp-ires-neo-dndb) using lipofectamine 2000 and selected by culturing in 200 microg/ml g418 for 6-8 days. g418 resistant fibroblasts and eg-like cells were used for ... | 2010 | 19144016 |
| fluoride-induced oxidative stress in rat's brain and its amelioration by buffalo (bubalus bubalis) pineal proteins and melatonin. | fluoride (f) becomes toxic at higher doses and induces some adverse effects on various organs, including brain. the mechanisms underlying the neurotoxicity caused by excess fluoride still remain unknown. the aims of this study were to examine f-induced oxidative stress (os) and role of melatonin (mel) and buffalo pineal proteins (pp) against possible f-induced os in brain of rats. the 24 rats were taken in present study and were divided into four groups: control, f, f + pp, and f + mel. the f gr ... | 2009 | 19159082 |
| pregnancies established from handmade cloned blastocysts reconstructed using skin fibroblasts in buffalo (bubalus bubalis). | handmade cloning (hmc), a simple, micromanipulation-free cloning technique, has been applied for the production of cloned embryos and offspring in many livestock species. the objective of the present study was to compare the effect of donor cell type on developmental competence of hmc embryos and to explore the possibility of establishing pregnancies using these embryos in buffalo. after technical optimization of the hmc procedure for in vitro development of cloned blastocysts, various donor cel ... | 2009 | 19168209 |
| the effect of addition of equine chorionic gonadotropin to a progesterone-based estrous synchronization protocol in buffaloes (bubalus bubalis) under tropical conditions. | poor estrus expression and anestrus decrease the reproductive efficiency of buffaloes. the objective of this study was to determine whether the addition of equine chorionic gonadotropin (ecg) to an estrous synchronization protocol and timed insemination could improve ovulation and pregnancy rates of anestrous buffalo cows under tropical conditions. the study population comprised 65 lactating murrah buffalo cows which were assigned to cidr (n=33) or cidr+ecg (n=32) treatment groups. cows in the c ... | 2009 | 19181376 |
| preliminary crystallographic study of hemoglobin from buffalo (bubalus bubalis): a low oxygen affinity species. | hemoglobin is a tetrameric, iron-containing metalloprotein, which plays a vital role in the transportation of oxygen from lungs to tissues and carbon dioxide back to lungs. though good amount of work has already been done on hemoglobins, the scarcity of data on three dimensional structures pertaining to low oxygen affinity hemoglobins from mammalian species, motivated our group to work on this problem specifically. herein, we report the preliminary crystallographic analysis of buffalo hemoglobin ... | 2009 | 19200047 |
| detection of coxiella burnetii in buffaloes aborted fetuses by is111 dna amplification: a preliminary report. | the aim of this study was to evaluate by pcr analyses the presence of coxiella burnetii infection in fetuses of water buffalo (bubalus bubalis) in the campania region (southern italy). samples were collected only from aborted fetuses and c. burnetii presence was evaluated by one-tube nested pcr amplification of the is111 repetitive element. of the 164 fetuses examined 14 (17.5%) were positive after dna amplification, showing that c. burnetii occurs in this population of water buffaloes. however, ... | 2009 | 19232419 |
| butylated hydroxytoluene inclusion in semen extender improves the post-thawed semen quality of nili-ravi buffalo (bubalus bubalis). | the study was carried out to evaluate the potential impact of butylated hydroxytoluene (bht) on the frozen-thawed semen quality of nili-ravi buffalo bulls. ejaculated bull semen was extended in a tris-citrate egg yolk extender containing various concentrations of bht (0.5, 1.0, 2.0 and 3.0mm). semen was frozen at -196 degrees c using 50 x 10(6) spermatozoa per 0.5 ml straws. five straws from each treatment were thawed to assess the semen quality in terms of sperm motility, viability, plasma memb ... | 2009 | 19246080 |
| improvement of liquid and frozen-thawed semen quality of nili-ravi buffalo bulls (bubalus bubalis) through supplementation of fat. | the aim of the study was to investigate the effects of dietary fat on quality of liquid and frozen-thawed semen of nili-ravi buffalo bulls. adult bulls (n=21) were fed a balanced ration (con; n=7) or the same ration either containing sunflower oil (sf-o; n=7) or whole sunflower seeds (sf-s; n=7) for 63 days. body weight and body condition score of each bull was recorded on days 0, 30 and 60 of the experiment. semen was collected on days 39, 46, 53 and 60, frozen by a fast method and stored at -1 ... | 2009 | 19246083 |
| expression of orexin a and its receptor 1 in the choroid plexuses from buffalo brain. | the hypothalamic peptide orexin a, deriving from the proteolytic cleavage of the precursor molecule prepro-orexin, has a wide range of physiological effects including the regulation of feeding behaviour, neuroendocrine functions, sleep-wake cycle, and energy homeostasis. lowered excretion of orexin a into the cerebrospinal fluid (csf) plays a pathological role in animal and human narcolepsy. altered levels of orexin a into the csf have been also found in numerous disorders of the central nervous ... | 2009 | 19250669 |
| identification and quantification of alphas1, alphas2, beta, and kappa-caseins in water buffalo milk by reverse phase-high performance liquid chromatography and mass spectrometry. | a method for the simultaneous quantitation of alpha(s1), alpha(s2), beta, and kappa-caseins in water buffalo (bubalus bubalis) milk using reverse phase high-performance liquid chromatography was developed. the molecular masses of the peaks separated by the described chromatographic protocol were determined by esi-ms. alpha(s1)- and kappa-caseins were found to be heteromorphic in several individual milk samples. in particular, alpha(s1)-casein showed two peaks with a molecular mass of 23,490 da a ... | 2009 | 19256489 |
| isolation of two cdnas encoding mhc-dqa1 and -dqa2 from the water buffalo, bubalus bubalis. | in the present study, we explored structural and functional variations and possible duplication of the major histocompatibility complex (mhc)-dqa gene in water buffalo (bubalus bubalis). two cdna sequences, amplified from one individual water buffalo, were designated as bubu-dqa1 (dqa*0101) and -dqa2 (dqa*2001). the percentage of nucleotide and amino acid similarity between bubu-dqa1 and -dqa2 revealed that these sequences display more similarity to alleles of respective dqa1 and dqa2 genes from ... | 2009 | 19272657 |
| bovine papillomavirus type 1 dna and e5 oncoprotein expression in water buffalo fibropapillomas. | papillomas and fibropapillomas may occur in the skin and in different organs in animals. ten different genotypes of bovine papillomavirus (bpv) have been identified. bpv-1 through bpv-10 are all strictly species-specific, but bpv-1/2 may also infect other species such as equids, inducing fibroblastic tumors. bpv-1 and bpv-2 are associated with fibropapillomas in cattle; these tumors are formed by excessive proliferation of virus-infected dermal fibroblasts and epidermal keratinocytes. nine water ... | 2009 | 19276046 |
| comparative moleculo-immunological analysis of swamp- and riverine-type water buffaloes responses. | this moleculo-epidemiological and immunological study through cytokine response assessment was done to know the dynamics of cytokines in the initiation, persistence and association to physiological changes of a particular pathogen in water buffaloes. this is important to understand the magnitude and behavior of disease progression. water buffalo blood samples gathered from different places in the philippines revealed a 9.4%, 27.6%, 10.3% and 4.4% prevalence of bovine viral diarrhea virus (bvdv), ... | 2009 | 19285880 |
| pharmacokinetics, urinary excretion and plasma protein binding of danofloxacin following intravenous administration in buffalo calves (bubalus bubalis). | pharmacokinetics, urinary excretion and plasma protein binding of danofloxacin was investigated in buffalo calves following intravenous administration at the dose rate of 1.25 mg/kg to select the optimal dosage regimen of danofloxacin. drug concentrations in plasma and urine were measured by microbiological assaying. in vitro plasma protein binding was determined employing the equilibrium dialysis technique. the distribution and elimination of danofloxacin were rapid, as indicated by values (mea ... | 2009 | 19296232 |
| heterogeneous shedding of brucella abortus in milk and its effect on the control of animal brucellosis. | to ascertain whether in brucella abortus-infected water buffalo herds, the number of newly infected animals could be reduced by culling superspreaders (the animals secreting > or =10(4) cfu per ml of milk). | 2009 | 19298512 |
| quantification of water buffalo (bubalus bubalis) cytokine expression in response to inactivated foot-and-mouth disease (fmd) vaccine. | this study describes the quantification of cytokine expression of vaccinated water buffaloes with fmd inactivated vaccine. using real-time pcr quantification assay, expression of th1 (il-2, il-12p40, ifngamma); th2 (il-4, il-10) and inflammatory (il-6, tnfalpha) cytokines were quantified weekly for the entire three-week duration of the experiment. it was noted that ifngamma, il-10 and tnfalpha had peaked on week three post-vaccination while the remaining cytokines peaked on the second week and d ... | 2009 | 19303121 |
| occurrence of beta-casein fragments in cold-stored and curdled river buffalo (bubalus bubalis l.) milk. | the safeguard of river buffalo mozzarella cheese, a protected designation of origin dairy product, has prompted an analytical study to trace the milk and curd used as raw material in cheesemaking. this is to prevent the illegal use of milk or curd from different geographical areas outside of those indicated in the official production protocol. for this purpose, we studied primary proteolysis occurring in fresh and frozen milk and curd to identify a molecular marker that could indicate the raw ma ... | 2009 | 19307613 |
| non-invasive methods for identifying oocysts of sarcocystis spp. from definitive hosts. | because the excreted sporocysts and/or oocysts of various species of sarcocystis may not be discriminated morphologically, we sought to validate a diagnostic technique based on variation in the 18s rdna sequence. oocysts and/or sporocysts from three taxa of sarcocystis were collected from human, feline, and canine definitive hosts that had fed upon meats infected with the muscle cysts of sarcocystis hominis, sarcocystis fusiformis and a species of sarcocystis from water buffalo that could not be ... | 2009 | 19336258 |
| comparative therapeutic effect of moxidectin, doramectin and ivermectin on psoroptes mites infestation in buffalo (bubalus bubalis). | the aim of the present study was to carry out comparative therapeutic effect of moxidectin pour on, doramectin and ivermectin on psoroptes infestation in buffalo. a total of 318 buffalo in 77 small scale herds suspected to have mange mites were examined clinically and parasitologically. fifty-three (16.66%) buffalo in 25 herds were recorded to be infested; 51 (16.35%) with psoroptic mites, and two (0.31%) with chorioptic mites. buffalo with psoroptic mites were randomly allocated into three grou ... | 2009 | 19347598 |
| shedding of brucella abortus rough mutant strain rb51 in milk of water buffalo (bubalus bubalis). | the objective of this study was to determine if brucella abortus rough mutant strain rb51 (srb51) is eliminated in buffalo milk. thirty brucella-free female buffaloes were used in this study: ten 4-5 years old were inoculated with the triple of the recommended calfhood dose of srb51 by subcutaneous route, ten 2-3 years old at the first lactation were previously vaccinated twice as calves with triple the recommended calf dose of rb51, while five 4-5 years old and five 2-3 years old not vaccinated ... | 2009 | 19362381 |
| lingual antimicrobial peptide expresses in buffalo mammary gland. | mammalian beta-defensins are innate host defense peptides with potential as a therapeutic agent. present study reports expression of beta-defensin mrna transcript in teat mucosal layer of bubalus bubalis, which is homologous to lingual antimicrobial peptide (lap) of cattle. the pcr-amplified and cloned full-length cdna encodes for a putative peptide of 64 residues ( approximately 7 kda) with pi of 11.324 and possess characteristic features of beta-defensin family including presence of six evolut ... | 2009 | 19370457 |
| fish-mapping comparison between river buffalo chromosome 7 and sheep chromosome 6: assignment of new loci and comparison with hsa4. | synchronized peripheral blood lymphocytes from both river buffalo (bbu) and sheep (oar) were treated for late incorporation of both brdu and h-33258 to obtain r-banded preparations to be used for fish-mapping. ovine bac-clones were hybridized for three days on slides pre-exposed to uv light after h-33258 staining. the following loci were mapped: gpr103 (bbu7q13, oar6q13), tram1l1(oar6q13dist), ppp3ca (bbu7q21, oar6q15), snca (oar6q17), ppargc1a(bbu7q23, oar6q17), ugdh (bbu7q25prox, oar6q22prox), ... | 2009 | 19372676 |
| different promoter usage for cyp19 gene expression in buffalo ovary and placenta. | aromatase is the key enzyme for estrogen biosynthesis and is coded by cyp19 gene. the expression of cyp19 gene is regulated in tissue-specific manner by alternate use of different promoters. in this study, we have analyzed tissue-specific expression and their regulation of cyp19 gene in preovulatory and postovulatory stages of buffalo (bubalus bubalis) ovary and placenta. rt-pcr analysis showed that the cyp19 gene expression was significantly (p<0.05) higher in granulosa cells of large follicles ... | 2009 | 19375426 |
| reproductive biotechniques in buffaloes (bubalus bubalis): status, prospects and challenges. | the swamp buffalo holds tremendous potential in the livestock sector in asian and mediterranean countries. current needs are the faster multiplication of superior genotypes and the conservation of endangered buffalo breeds. recent advances in assisted reproductive technologies, including in vitro embryo production methodologies, offer enormous opportunities to not only improve productivity, but also to use buffaloes to produce novel products for applications to human health and nutrition. the us ... | 2009 | 19383257 |
| efficiency of scnt buffalo (bubalus bubalis) embryos in different culture medium and analysis of mrna expression of insulin-like growth factors during embryogenesis. | growth factors in culture media are known to affect the embryo production rates in in vitro production cultures. to improve the efficiency of somatic cell nuclear transfer (scnt) derived embryos in indian buffaloes (bubalus bubalis), embryos were cultured in three different culture mediums viz. group-a; tcm-199 + fbs, group-b; tcm-199 + poly vinyl alcohol (pva) and group-c; cr1aa + bsa. embryo production rate and expression level of insulin-like growth factor genes (igf-1, igf-1r, igf-2 and igf- ... | 2010 | 19392670 |
| high-resolution haplotype block structure in the cattle genome. | the bovine hapmap consortium has generated assay panels to genotype ~30,000 single nucleotide polymorphisms (snps) from 501 animals sampled from 19 worldwide taurine and indicine breeds, plus two outgroup species (anoa and water buffalo). within the larger set of snps we targeted 101 high density regions spanning up to 7.6 mb with an average density of approximately one snp per 4 kb, and characterized the linkage disequilibrium (ld) and haplotype block structure within individual breeds and grou ... | 2009 | 19393054 |
| fatal onion (allium cepa) toxicosis in water buffalo (bubalus bubalis). | toxicosis caused by the ingestion of onion (allium cepa) by 5 water buffalo (bubalus bubalis) occurred in the district of caçador, santa catarina, brazil. the water buffalo died after ingestion of a large quantity of onion that had been left in the pasture. clinical signs started 8 days postingestion and were characterized by pale mucous membranes, lethargy, and dark urine. at necropsy, pieces of onions were found in the rumen of 1 animal. the carcass smelled strongly of onion, and the kidneys a ... | 2009 | 19407101 |
| effect of sodium nitroprusside, a nitric oxide donor, and aminoguanidine, a nitric oxide synthase inhibitor, on in vitro development of buffalo (bubalus bubalis) embryos. | 2010 | 19416489 | |
| light and scanning electron microscopic studies of oviductal epithelium in thai swamp buffalo (bubalus bubalis) at the follicular and luteal phases. | the purpose of this study was to investigate the morphological changes in the epithelium of thai swamp buffalo oviducts at the follicular and luteal phases by histological technique and scanning electron microscopy. the samples from the infundibulum, ampulla, isthmus and uterotubal junction (utj) of the oviduct were taken immediately after slaughter at the local abattoir. noticeable cyclic changes were observed on the epithelial surface of the infundibulum and ampulla, but few changes were prese ... | 2009 | 19416492 |
| a point mutation in the splice donor site of intron 7 in the alphas2-casein encoding gene of the mediterranean river buffalo results in an allele-specific exon skipping. | 2009 | 19422363 | |
| helmintic infections in water buffaloes on italian farms: a spatial analysis. | the present paper reports the results of a cross-sectional survey aimed at obtaining up-to-date information on the spatial distribution of different groups and/or species of helminths in water buffaloes from central italy. geographical information systems (gis) and spatial analysis were used to plan the sampling procedures, to display the results as maps and to detect spatial clusters of helminths in the study area. the survey was conducted on 127 water buffalo farms, which were selected in the ... | 2009 | 19440965 |
| tolerance to water buffalo milk in a child with cow milk allergy. | 2009 | 19441608 | |
| transplacental transfer of iron in the water buffalo (bubalus bubalis): uteroferrin and erythrophagocytosis. | the objectives of this investigation were to understand transplacental transport of iron by secreted uteroferrin (uf) and haemophagous areas of water buffalo placenta and clarify the role(s) of blood extravasation at the placental-maternal interface. placentomes and interplacentomal region of 51 placentae at various stages of gestation were fixed, processed for light and transmission electron microscopy, histochemistry and immunohistochemistry. haemophagous areas were present in placentomes coll ... | 2010 | 19453495 |
| classification of new bvdv isolates from philippine water buffalo using the viral e2 region. | characterization of bovine viral diarrhea virus (bvdv) isolates has been focused of several studies this last decade. until now lots of new strains are being unfolded maybe due to the viral fast mutation ability. as we focused our research on water buffalo immunology, we were able to identify a probable new bvdv isolates. rna was extracted from water buffalo blood in the philippines. the extracted rna was reverse-transcribed and synthesized cdna. oligonucleotide primers from the viral e2 region ... | 2009 | 19455512 |
| phylogenetic analysis and comparison between cow and buffalo (including egyptian buffaloes) mitochondrial displacement-loop regions. | mitochondrial dna (mtdna) analysis has been used extensively for phylogenetic analysis studies and systematics. the displacement loop (d-loop) region inside the mtdna is a non-coding part whose analysis can indicate variations between closely related populations. this paper reports for the first time the characterization and analysis of the complete sequence of the d-loop region from egyptian buffaloes and analysis in conjunction with previously published indian and european bubalus bubalis and ... | 2008 | 19462514 |
| cryotop vitrification of buffalo (bubalus bubalis) in vitro matured oocytes: effects of cryoprotectant concentrations and warming procedures. | the aim of the work was to evaluate the in vitro developmental competence of in vitro-matured buffalo oocytes after cryotop vitrification (ctv) and in vitro fertilization (ivf). to optimize parameters, two cryoprotectant (cp) concentrations and two warming-dilution procedures were applied. oocytes were vitrified in 16.5% ethylene glycol (eg), 16.5% dimethylsulphoxide (dmso) and 0.5 m sucrose in groups a and c, and in higher cp concentrations (20% eg, 20% dmso and 0.5 m sucrose) in groups b and d ... | 2010 | 19473307 |
| effects of exposure to heavy metals on viability, maturation, fertilization, and embryonic development of buffalo (bubalus bubalis) oocytes in vitro. | the aim of the present study was to examine the effect of heavy metals, cadmium and lead, on buffalo oocyte viability and in vitro development. oocytes were aspirated from ovaries of slaughtered buffaloes. only viable and metabolically active oocytes with more than three layers of cumulus cell layers and homogeneous ooplasm were selected. effects of nine concentrations (0, 0.005, 0.05, 0.5, 1.0, 1.5, 2.5, 5, and 10 microg/ml) of cadmium or lead on buffalo oocyte viability, morphological abnormit ... | 2010 | 19475365 |
| expression and immunohistochemical localization of the neonatal fc receptor (fcrn) in the mammary glands of the egyptian water buffalo. | although a marginal placental transfer of maternal immunoglobulin (ig) has been demonstrated in buffalo, the colostrum still provides the main source of immune components and nutrients to neonate buffalo calves. the neonatal fc receptor (fcrn) transports maternal ig across the gut wall and is involved in the transport of igg in the mammary gland. in this study we used rt-pcr to examine the gene expression of fcrn in the mammary gland during several physiological states of the egyptian water buff ... | 2010 | 19481783 |
| dominance of methanomicrobium phylotype in methanogen population present in murrah buffaloes (bubalus bubalis). | to study the diversity of rumen methanogens in murrah buffaloes (bubalus bubalis) from north india by using 16s rrna gene libraries obtained from the pooled rumen content from four animals and using suitable software analysis. | 2009 | 19486281 |
| first isolation of escherichia coli o157:h7 from faecal and milk specimens from anatolian water buffaloes (bubalus bubalus) in turkey. | three hundred rectal faecal samples and 213 raw milk samples obtained from the tanks and containers were examined using standard cultural methods. escherichia coli o157:h7 was isolated from 11 (3.7%) of 300 faecal samples and 3 (1.4%) of 213 raw milk samples. it was determined that 8 (73%) of e. coli o157:h7 strains isolated from faecal samples originated from water buffaloes younger than 2 years of age and 3 (27%) from 2-year-old and older water buffaloes. this is the 1st isolation of escherich ... | 2008 | 19496315 |
| effects of fsh and lh on steroid production by buffalo (bubalus bubalis) granulosa cells cultured in vitro under serum-free conditions. | the objective of this study was to examine the effects of fsh and lh on oestradiol-17β and progesterone production by buffalo granulosa cells cultured under serum-free conditions. granulosa cells (3 × 10(5) ) from small (≤ 5 mm diameter) follicles were cultured for up to 4 days in 48-well plates coated with 3.3 μg/cm(2) fibronectin in dulbecco's modified eagle's medium (dmem) : nutrient mixture f-12 ham (1 : 1 ratio) supplemented with 10(-7) m androstenedione, 5 μg/ml human apo-transferrin and 0 ... | 2010 | 19497023 |
| assessment of dna damage during in vitro development of buffalo (bubalus bubalis) embryos: effect of cysteamine. | comet assay was used in the present study to examine dna damage to buffalo oocytes and embryos during in vitro culture. embryos were produced in vitro from oocytes obtained from slaughterhouse ovaries in presence of cysteamine (ivm and ivc media supplemented with 50 and 100 μm, respectively) or in its absence (controls). compared to controls, cysteamine supplementation increased (p < 0.01) cleavage rate and proportion of oocytes that developed to 8- to 16-cell stage. the incidence of dna damage ... | 2010 | 19515031 |
| detection of nontuberculous mycobacteria from water buffalo raw milk in brazil. | milk is an important nutritional source to man and water buffalo raw milk is used to produce mozzarella cheese. products from unpasteurized milk have been associated with certain infectious diseases and can carry pathogenic mycobacteria. nontuberculous mycobacteria (ntm) are emerging pathogens causing opportunistic infections in humans and animals. the objectives of this study were to demonstrate the presence of mycobacteria in water buffaloes' milk and to determine their role as possible source ... | 2009 | 19527843 |
| temporal changes in endogenous estrogens and expression of behaviors associated with estrus during the periovulaory period in murrah buffaloes (bubalus bubalis). | the objective of this study were (1) to establish the duration of behavioral estrus signs and timing of ovulation in murrah buffaloes (n = 10) and (2) to determine relationship between behavioral estrus signs with change in plasma estrogen concentrations. estrus and its behavioral signs were detected at hourly intervals by visual observations, per recta examination of genitalia and bull parading four times in a day for 30 minutes each. among the behavioral signs of estrus, swollen vulva (80%) wa ... | 2010 | 19544005 |
| identification of novel allelic variants of integrin beta 2 (itgb2) gene and screening for bubaline leukocyte adhesion deficiency syndrome in indian water buffaloes (bubalus bubalis). | a fragment of 570 bp corresponding to exon 5 and 6 of integrin beta 2 (itgb2) gene was amplified for screening d128g mutation in one hundred and fifty two buffaloes (bubalus bubalis) which causes bovine leukocyte adhesion deficiency syndrome (blad) in cattle, as well as to ascertain polymorphism. taqi pcr-rflp revealed no such mutation thus indicating the absence of bubaline leukocyte adhesion deficiency (bulad) allele in animals under study. however, the polymorphism studies using mspi restrict ... | 2009 | 19544212 |
| molecular cloning and characterization of cdna encoding buffalo (bubalus bubalis) cytochrome p450 aromatase in the ovary. | one of the predominant causes of poor reproduction in buffaloes is low levels of ovarian estrogens. a rate limiting enzyme in estrogen biosynthesis is cytochrome p450 aromatase (p450 arom), the product of cyp19 gene. in the present study cyp19 cdna was cloned and its 5'utr was characterized by 5'race in granulosa cells of large follicles. cyp19 transcripts with four different 5'utrs (206, 114, 90 and 3 bases) were found in buffalo granulosa cells of large ovarian follicles. interestingly, a pred ... | 2009 | 19544214 |
| clinical and biochemical studies on theileria annulata in egyptian buffaloes (bubalus bubalis) with particular orientation to oxidative stress and ketosis relationship. | this study was carried out on 68 theileria annulata naturally infected buffaloes in addition to 25 parasitologically free buffaloes distributed in small herds at dakahlia and gharbya governorates, egypt, to demonstrate the clinical picture associated with theileriosis in this buffaloes with particular emphasis to the oxidative stress and ketosis relationship. clinical signs recorded in infected buffaloes were in the form of fever, enlargement of one or more lymph node, ocular discharge, corneal ... | 2009 | 19559534 |
| effects of the seminal plasma zinc content and catalase activity on the semen quality of water buffalo (bubalus bubalis) bulls. | in order to determine zinc and catalase content of seminal plasma in the buffalo and to study their associations with the semen characteristics, 54 semen samples were collected from 10 buffalo bulls; semen volume and sperm concentration, gross and progressive motility and viability were evaluated, seminal plasma was then harvested by centrifugation and its zinc content was estimated by atomic absorption spectrophotometer and its catalase activity determined by using a commercial kit. the zinc co ... | 2009 | 19579933 |
| expressional dynamics of minisatellite 33.15 tagged spermatozoal transcriptome in bubalus bubalis. | transcriptionally quiescent spermatozoa have been established to be a repository of mrna coding for several functionally essential cellular proteins. this entourage of mrna is envisaged to be involved in post-fertilization and early embryogenesis. minisatellites tagged with mrna transcripts have been implicated with gene organization, regulation and function. however, the organization and expression of the minisatellite tagged transcript diversity, particularly in spermatozoa, remains unclear. | 2009 | 19583837 |
| optimization of embryo culture conditions for increasing efficiency of cloning in buffalo (bubalus bubalis) and generation of transgenic embryos via cloning. | cloning in bovine species is marred by low efficiency of blastocyst formation. any increase in the efficiency of blastocyst formation upon nuclear transfer will greatly enhance the efficiency of cloning. in the present study, the effect of various media, protein sources, and growth factors on the development of cloned buffalo embryos was evaluated. among various combinations tested, culture of cloned embryos in tcm-199 media on the feeder layer of buffalo oviductal epithelial cells (boec) in the ... | 2009 | 19594388 |
| improvement of in vitro oocyte maturation with lectin supplementation and expression analysis of cx43, gdf-9, fgf-4 and fibronectin mrna transcripts in buffalo (bubalus bubalis). | to optimize in vitro maturation (ivm) of quality oocytes for embryo production through ivf and scnt. | 2009 | 19629675 |
| bacterial diversity in the rumen of gayals (bos frontalis), swamp buffaloes (bubalus bubalis) and holstein cow as revealed by cloned 16s rrna gene sequences. | libraries of rumen bacterial 16s rrna gene sequences of gayals (bos frontalis) and swamp buffaloes (bubalus bubalis) were cloned and sequenced in the present work to compare the bacterial diversity with the third published library of holstein cow. sequence similarity of 97% was used as the definition of operational taxonomic unit (otu). the majority of the 470 sequences retrieved fell into the phyla of low g + c subdivision (329 sequences) and cytophaga-flexibacter-bacteroides (cfb, 123 sequence ... | 2010 | 19662514 |
| loop-mediated isothermal amplification (lamp) detection of babesia orientalis in water buffalo (bubalus babalis, linnaeus, 1758) in china. | loop-mediated isothermal amplification (lamp) is a rapid method with high specificity and efficiency under isothermal condition using a set of four specifically designed primers that recognize six distinct sequences on the target gene. in this study, a lamp method was developed for specific detection of babesia orientalis in water buffalo (bubalus babalis, linnaeus, 1758). four primers were designed from the v4 hypervariable region of the 18s rrna gene of b. orientalis. blood samples were collec ... | 2009 | 19665847 |
| sequence analysis of morbillivirus cd150 receptor-signaling lymphocyte activation molecule (slam) of different animal species. | signaling lymphocyte activation molecule-slam (cd150) molecule has been reported as a putative receptor for most morbilliviruses for their respective host species. in this study, we determined the complete nucleotide sequence of the gene coding for the morbillivirus receptor-slam from the four species, namely, goat (capra hircus), sheep (ovis aries), indian cattle (bos indicus), and buffalo (bubalus bubalis). the nucleotide (nt) open reading frame sequence of slam gene in all the four species st ... | 2009 | 19669672 |
| risk factors of infestation by psoroptes spp. mites in buffalo (bubalus bubalis) at smallholder farms in the nile delta region, egypt. | the aim of the present study was to explore the prevalence and potential risk factors associated with infestation by psoroptes spp. mites in buffalo at smallholder farms in the nile delta region, egypt. a total of 318 out of 1234 buffalo in 77 small scale herds were examined. the age of buffalo varied from three months to seven years. in all, 53 (16.66%) buffalo were recorded to be infested; 51 (16.35%) with psoroptes spp. mites and two cases (0.31%) with chorioptes spp. mites. multivariate logi ... | 2010 | 19688306 |
| factors affecting laboratory production of buffalo embryos: a meta-analysis. | in vitro fertilization (ivf) provides an excellent and inexpensive source of embryos for carrying out basic research on developmental physiology, farm animal breeding, and for commercial applications. meta-analysis of the results from different publications rather than a narrative review may provide a current status of this technology in buffalo (bubalus bubalis). in order to gain an idea of the factors affecting the ivf in buffalo, a review of the various studies conducted on buffalo ivf and a ... | 2009 | 19695691 |
| verocytotoxin-producing escherichia coli o26 in raw water buffalo (bubalus bubalis) milk products in italy. | escherichia coli 026 is known as a verocytotoxin-producing e. coli (vtec) organism that causes severe foodborne diseases such as hemorrhagic colitis and hemolytic uremic syndrome. although cattle are the most important reservoir of vtec, only a few reports on the role of water buffalo (bubalus bubalis) as a reservoir of vtec and on the presence of these organisms in their milk are available. however, in southern italy, where water buffalo are intensively reared, an outbreak of hemolytic uremic s ... | 2009 | 19722404 |
| validation and application of an immunofluorimetric assay for detection of serum free triiodothyronine and free thyroxine concentrations in buffalo (bubalus bubalis) under various physiological conditions. | the current study was designed to validate an immunofluorimetric method to measure free triiodothyronine (ft3) and free thyroxine (ft4) concentrations in the serum of buffalo and to develop a better understanding of thyroid metabolism in buffalo (bubalus bubalis) of different ages and reproductive statuses. free triiodothyronine and ft4 were determined in sera from 4 groups of 10 animals, each representing 5-10-year-old lactating buffalo, 2-3-year-old first calf heifers, 1-1.5-year-old virgin he ... | 2009 | 19737762 |
| ion, protein, phospholipid and energy substrate content of oviduct fluid during the oestrous cycle of buffalo (bubalus bubalis). | the aim of this research was to analyse the composition of oviduct fluid (odf) in buffalo cows at different oestrous cycle phases to fulfil the requirements of buffalo embryos in vitro. odf was collected by chronic cannulation from three cows that were synchronized by administering a synthetic prostaglandin. based on hormonal profiles, the pre-ovulatory, ovulatory, post-ovulatory and luteal phases of the oestrous cycle were defined. the volume of odf produced (ml/24 h) was influenced by the oest ... | 2010 | 19761531 |
| characterization of spliced leader genes of trypanosoma ( megatrypanum) theileri: phylogeographical analysis of brazilian isolates from cattle supports spatial clustering of genotypes and parity with ribosomal markers. | trypanosoma (megatrypanum) theileri from cattle and trypanosomes of other artiodactyls form a clade of closely related species in analyses using ribosomal sequences. analysis of polymorphic sequences of a larger number of trypanosomes from broader geographical origins is required to evaluate the clustering of isolates as suggested by previous studies. here, we determined the sequences of the spliced leader (sl) genes of 21 isolates from cattle and 2 from water buffalo from distant regions of bra ... | 2010 | 19765336 |
| diagnosis of sarcocystis spp. in cattle (bos taurus) and water buffalo (bubalus bubalis) in northern vietnam. | our aim was to develop a method for species diagnosis and to obtain data on the prevalence of sarcocystis infections in cattle and water buffalo in the son la province of northern vietnam. meat samples of naturally infected animals were examined by light and electron microscopy as well as by molecular methods. a pcr of part of the 18s rdna gene followed by rflp analysis was modified to detect infections with different sarcocystis spp. in cattle and water buffaloes slaughtered in the son la provi ... | 2009 | 19783101 |
| occurrence of aflatoxin m1 in raw milk of five dairy species in ahvaz, iran. | during november 2007 to december 2008, 311 samples of raw milk from cow, water buffalo, camel, sheep, and goat were collected in the ahvaz (southwest iran). all of the samples were analyzed for presence of aflatoxin m1 (afm1) by competitive elisa technique. afm1 was found in 42.1% of the samples by average concentration of 43.3+/-43.8 ng/kg. the incidence rates of afm1 in raw cow, water buffalo, camel, sheep, and goat milks were, 78.7%, 38.7%, 12.5%, 37.3%, and 27.1%, respectively. the concentra ... | 2010 | 19786054 |
| an inter-subspecies cloned buffalo (bubalus bubalis) obtained by transferring of cryopreserved embryos via somatic cell nuclear transfer. | the aim of this study was to explore the feasibility of cryopreservation of inter-subspecies cloned embryos in buffalo. in our experiment, river buffalo ear fibroblast nucleus was fused into swamp buffalo oocyte cytoplasm. the blastocyst formation rate for nuclear transfer of freshly thawed cells was not different from those of growing cells, confluent or serum-starved cells. a total of 122 cloned blastocysts derived from cryopreserved fibroblasts were cryopreserved and thawed, 37 were survived, ... | 2010 | 19788521 |
| genetic variation and relationships among turkish water buffalo populations. | the genetic variation and relationships among six turkish water buffalo populations, typical of different regions, were assessed using a set of 26 heterologous (bovine) microsatellite markers. between seven and 17 different alleles were identified per microsatellite in a total of 254 alleles. the average number of alleles across all loci in all the analysed populations was found to be 12.57. the expected mean heterozygosity (h(e)) per population ranged between 0.5 and 0.58. significant departure ... | 2010 | 19799598 |
| testicular development and establishment of spermatogenesis in nili-ravi buffalo bulls. | fifteen longitudinally reared nili-ravi buffalo bulls (bubalus bubalis) were slaughtered at 1, 6, 12, 18, and 24 mo of age (n=3 per group) to observe testicular development and to examine qualitatively the establishment of spermatogenesis. with the age held constant, scrotal circumference and testes weight were correlated (0.95; p<0.05). testes weight increased from 3.5+/-0.7 at 1 mo of age to 185+/-30g at 24 mo of age. seminiferous tubules diameter developed in a linear fashion (57microm at 1 m ... | 2010 | 19815266 |
| effect of donor cell types on developmental potential of cattle (bos taurus) and swamp buffalo (bubalus bubalis) cloned embryos. | this study investigated the effect of donor cell types on the developmental potential and quality of cloned swamp buffalo embryos in comparison with cloned cattle embryos. fetal fibroblasts (ffs), ear fibroblasts (efs), granulosa cells (gcs) and cumulus cells (ccs) were used as the donor cells in both buffalo and cattle. the cloned cattle or buffalo embryos were produced by fusion of the individual donor cells with enucleated cattle or buffalo oocytes, respectively. the reconstructed (cloned) em ... | 2010 | 19815984 |
| detection of mycobacterium avium subsp. paratuberculosis in tissue samples of cattle and buffaloes. | tissue samples were collected at random from cattle (bos taurus) and buffalo (bubalus bubalis) from an abattoir of the district of lahore and were analyzed for the presence of mycobacterium avium subsp. paratuberculosis and mycobacterium bovis through acid-fast staining and polymerase chain reaction (pcr). body condition of animals and diarrhea were recorded. most of the animals were emaciated. diarrhea was noticed in 15.6% of buffaloes and 19.2% of cattle. intestinal pathology was observed in 2 ... | 2010 | 19816784 |
| the molecular phylogeny of uterine serpins and its relationship to evolution of placentation. | uterine serpins (uss), designated as serpina14, are expressed in the endometrium in response to progesterone. all species identified as having uss exhibit epitheliochorial placentation and are in the ruminantia and suidae orders of the laurasiatheria superorder. the objective was to identify us genes in species within and outside laurasiatheria and evaluate whether evolution of the us gene was associated with development of the epitheliochorial placenta. through queries of nucleotide and genomic ... | 2010 | 19825977 |
| improvement in buffalo (bubalus bubalis) spermatozoa functional parameters and fertility in vitro: effect of insulin-like growth factor-i. | the aim of the current study was to assess the effect of insulin-like growth factor-i (igf-i; 100 ng/ml) on buffalo (bubalus bubalis) sperm functional parameters related to in vitro fertilization. the acrosin activity (the mean diameter of halo formation in micrometers) was significantly higher in the igf-i group (14.17 +/- 1.51) compared with that in the control group (9.50+/-0.36) at 2h incubation. the mitochondrial membrane potential (per cent) was significantly higher in the igf-i group comp ... | 2010 | 19828187 |
| immune response and viral persistence in indian buffaloes (bubalus bubalis) infected with foot-and-mouth disease virus serotype asia 1. | despite their potential role in the spread of foot-and-mouth disease (fmd), the immune response and viral persistence in fmd virus (fmdv)-infected indian buffaloes (bubalus bubalis) have been unexplored. we found similar kinetics of neutralizing antibody responses in the sera and secretory fluids of buffaloes following experimental fmdv asia 1 infection, but the lymphocyte-proliferative response in infected buffaloes was of low magnitude. despite inducing a significant systemic and secretory imm ... | 2009 | 19828770 |
| fast isoelectric focusing and antipeptide antibodies for detecting bovine casein in adulterated water buffalo milk and derived mozzarella cheese. | plasmin hydrolysis of water buffalo casein (cn) can liberate a peptide comigrating with bovine gamma(2)-cn. occurrence of this peptide may lead to false-positive detection of cow's milk for a genuine water buffalo cheese when it is analyzed by applying a fast version of the european official method for detecting bovine casein in water buffalo cheese. after isoelectric focusing of cn plasminolysates, performed according to the official method, immunoblot analysis with antipeptide antibodies was a ... | 2009 | 19831418 |
| purification, characterization and expression kinetics of heat shock protein 70 from bubalus bubalis. | the present work on bubalus bubalis (buffalo) was designed to study heat shock protein 70 (hsp70) induction in lymphocytes, its purification and characterization. hsp70 induction and expression kinetics at different temperatures and time durations were also studied. hsp70 purification was carried out by immunoaffinity chromatography using adenosine di-phosphate (adp-agarose column) and the characterization of the purified protein was done using western blotting by mouse monoclonal anti-hsp70. th ... | 2010 | 19836035 |
| immunohistochemical studies of the epididymal duct in egyptian water buffalo (bubalus bubalis). | using immunohistochemistry (ihc), this study aimed to evaluate the regional distribution pattern of some biologically active proteins in the epididymis of egyptian water buffalo and to determine the structural-functional relationships of the different epididymal structures. wax-embedded sections from different regions of the epididymal duct from adult, clinically healthy, buffalo bulls were used. primary antibodies against angiotensin converting enzyme (ace), s-100, galactosyltransferase (galtas ... | 2011 | 19836061 |
| microsatellite markers of water buffalo, bubalus bubalis--development, characterisation and linkage disequilibrium studies. | microsatellite markers are highly polymorphic and widely used in genome mapping and population genetic studies in livestock species. river buffalo, bubalus bubalis is an economically important livestock species, though only a limited number of microsatellite markers have been reported thus far in this species. | 2009 | 19843347 |
| antimicrobial resistance in gram-positive bacteria from timorese river buffalo (bubalus bubalis) skin microbiota. | the timorese river buffalo (bubalus bubalis) plays a major role in the east timor economy, as it is an important source of animal protein in human nutrition. they are widely spread throughout the country and are in direct contact with the populations. in spite of this proximity, information on their microbiota is scarce. this work aimed at characterizing the skin microbiota of the east timorese river buffalo and its antimicrobial resistance profile. skin swab samples were taken from 46 animals i ... | 2010 | 19898998 |
| glutathione concentration and gamma-glutamyltransferase activity in water buffalo colostrum. | evidence is presented that the buffalo mammary gland contains enzymes that catalyse the synthesis and utilization of glutathione. a significant, inverse correlation (r = 0.79) was detected between colostrum gamma-glutamyltransferase (ggt) and glutathione (gsh), suggesting that the enzyme uses gsh as a substrate for its activity. a similar trend was shown in mammary gland homogenates (r = 0.75). our results show that gsh is secreted into buffalo colostrum and suggest that the enzyme ggt degrades ... | 2010 | 19906130 |
| detection of prion gene promoter and intron1 indel polymorphisms in anatolian water buffalo (bubalus bubalis). | bovine spongiform encephalopathy (bse) is a fatal disease caused by miss folded prion protein. studies in the cattle, comparing genetic data from bse diseased and healthy animals have shown that indel polymorphisms in the promoter and intron 1 of prnp gene were associated with disease susceptibility. several studies were conducted to find out allele and genotypic frequencies of indel polymorphisms in promoter and intron 1 of the cattle prnp gene. unlike domestic cattle and bison, no indel polymo ... | 2009 | 19912420 |
| short communication: detection of human torque teno virus in the milk of water buffaloes (bubalus bubalis). | forty-four raw milk and 15 serum samples from 44 healthy water buffaloes reared in caserta, southern italy, the most important region in europe for buffalo breeding, were examined to evaluate the presence of torque teno viruses (ttv) using molecular tools. furthermore, 8 pooled pasteurized milk samples (from dairy factories having excellent sanitary conditions) and 6 mozzarella cheese samples were also tested. four of the cheese samples were commercial mozzarella cheese; the remaining 2 were pre ... | 2009 | 19923596 |
| microsatellite-based genetic monitoring to detect cryptic demographic bottleneck in indian riverine buffaloes (bubalus bubalis). | the present study was undertaken to evaluate different indian riverine buffalo breeds (bubalus bubalis) for mutation drift equilibrium and occurrence of any recent genetic bottleneck. a total of 330 animals from seven different breeds were analyzed with a set of 24 heterologous microsatellite markers. three different tests revealed significant heterozygosity excess in all the seven buffalo breeds studied when assumed under infinite alleles model of microsatellite evolution, while it was the reve ... | 2010 | 19936960 |
| specific intracellular localization and antiviral property of genetic and splicing variants in bovine mx1. | in bovine mx1, only an amino acid substitution between ile and met at position 120 was detected by the nucleotide sequence and mismatched pcr-rflp technique. the ile variant was assumed to distribute mainly in the bovine population since the gene frequency was 79.3%. furthermore, we cloned water buffalo mx1 cdna, which showed 51 nucleotide and 20 amino acid substitutions in comparison with that of the cow. another kind of mx1 cdna, bovine mx1b cdna, was found and it was deduced to cause 27 amino ... | 2009 | 19951175 |
| changes in endogenous estrogens and expression of behaviors associated with estrus during the periovulatory period in heatsynch treated murrah buffaloes (bubalus bubalis). | the objective of this study was (1) to establish the duration of behavioral estrus signs and timing of ovulation in murrah buffaloes (n = 10) and (2) to determine relationship between behavioral estrus signs with change in plasma estrogen concentrations in animals treated with heatsynch protocol. estrus and its behavioral signs were detected at hourly intervals by visual observations per rectal examination of genitalia and bull parading four times in a day for 30 min each. among the behavioral s ... | 2010 | 19997777 |
| seven novel single nucleotide polymorphisms identified within river buffalo (bubalus bubalis) lactoferrin gene. | the present study aimed at identifying single-nucleotide polymorphic (snp) sites in different coding and non-coding regions of lactoferrin gene in indian riverine buffaloes. a total of 102 animals from six different river buffalo breeds were screened at six bubaline lactoferrin gene loci. single-strand conformation polymorphism (sscp) analysis revealed monomorphic patterns at three loci ltfe2, ltfe11, and ltfe14 while a total of eight distinct patterns were observed in the other three loci viz. ... | 2010 | 20012192 |
| comparative rh maps of the river buffalo and bovine y chromosomes. | radiation hybrid maps were constructed for river buffalo and cattle y chromosomes. a total of 41 cattle-derived y-chromosome molecular markers were selected and tested with 2 previously described 5,000-rad whole-genome radiation hybrid (rh) panels (river buffalo - bburh(5000) and cattle - btarh(5000)) for generation of maps. among the initial 41 selected markers, a subset of 26 markers generated pcr products suitable for scoring with the bburh(5000) panel. of these, 19 markers (73%) were distrib ... | 2009 | 20016162 |
| comparative mapping and genomic annotation of the bovine oncosuppressor gene wwox. | wwox (ww domain-containing oxidoreductase) is the gene mapping at fra16d hsa16q23.1, the second most active common fragile site in the human genome. in this study we characterized at a detailed molecular level wwox in the bovine genome. first, we sequenced cdna from various tissues and obtained evidence in support of a 9-exon structure for the gene, similar to the human gene. then, we recovered bacs using exon tags and annotated the gene to a >1-mb genomic region of bta18 using the btau 4.0 geno ... | 2009 | 20016169 |