Publications
Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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an inside look at the sensory biology of triatomines. | although kissing bugs (triatominae: reduviidae) are perhaps best known as vectors of chagas disease, they are important experimental models in studies of insect sensory physiology, pioneered by the seminal studies of wigglesworth and gillet more than eighty years ago. since then, many investigations have revealed that the thermal, hygric, visual and olfactory senses play critical roles in the orientation of these blood-sucking insects towards hosts. here we review the current knowledge about the ... | 2017 | 27840287 |
screening of fungi for biological control of a triatomine vector of chagas disease: temperature and trypanosome infection as factors. | entomopathogenic fungi have been investigated as an alternative tool for controlling various insects, including triatomine vectors of the protozoan trypanosoma cruzi, the etiological agent of chagas disease. here we tested the pathogenicity and virulence of ten isolates of the fungi metarhizium spp. and beauveria bassiana against rhodnius prolixus and found all of the isolates to be virulent. we used two isolates (urpe-11 metarhizium anisopliae and ent-1 beauveria bassiana) for further screening ... | 2016 | 27855217 |
modeling the effects of palm-house proximity on the theoretical risk of chagas disease transmission in a rural locality of the orinoco basin, colombia. | chagas disease is a major public health concern in latin america and it is transmitted by insects of the subfamily triatominae, including rhodnius spp. since palm trees are ubiquitous in colombia and a habitat for rhodnius spp., the presence of palms near villages could increase contact rates between vectors and humans. therefore, knowing whether a relationship exists between the proximity of palms to villages and the abundance and distribution of vectors therein, may be critical for chagas dise ... | 2016 | 27863520 |
kissing bugs can generalize and discriminate between different bitter compounds. | animals make use of contact chemoreception structures to examine the quality of potential food sources. during this evaluation they can detect nutritious compounds that promote feeding and recognize toxins that trigger evasive behaviors. although animals can easily distinguish between stimuli of different gustatory qualities (bitter, salty, sweet, etc.), their ability to discriminate between compounds of the same quality may be limited. numerous plants produce alkaloids, compounds that elicit av ... | 2016 | 27865772 |
rhodnius prolixus: from physiology by wigglesworth to recent studies of immune system modulation by trypanosoma cruzi and trypanosoma rangeli. | this review is dedicated to the memory of professor sir vincent b. wigglesworth (vw) in recognition of his many pioneering contributions to insect physiology which, even today, form the basis of modern-day research in this field. insects not only make vital contributions to our everyday lives by their roles in pollination, balancing eco-systems and provision of honey and silk products, but they are also outstanding models for studying the pathogenicity of microorganisms and the functioning of in ... | 2017 | 27866813 |
lethal and sublethal effects of essential oil of lippia sidoides (verbenaceae) and monoterpenes on chagas' disease vector rhodnius prolixus. | the aim of this study was to identify the composition of the essential oil from leaves of lippia sidoides (eols), a typical shrub commonly found in the dry northeast of brazil, popularly known as "alecrim-pimenta". additionally, we investigated the nymphicidal, ovicidal, phagoinhibitory and excretion effects of eols, its major constituent thymol and its isomer carvacrol, on fourth instar nymphs and eggs of rhodnius prolixus, the chagas' disease vector. the nymphicidal and ovicidal activity of th ... | 2017 | 27878214 |
genetic relationships and spatial genetic structure among populations of rhodnius prolixus (hemiptera: reduviidae) in colombia and venezuela based on mitochondrial cytochrome-b sequences. | one hundred twenty rhodnius prolixus (stal) (hemiptera: reduviidae) specimens from 6 colombian departments and 1 venezuelan state had 594-bp of the mitochondrial cytochrome-b gene sequenced to improve the understanding of evolutionary processes that shape the main vector of chagas disease. the levels of genetic diversity for this species were low-medium with reference to other bugs. the genetic heterogeneity among the populations was very limited which means there has been extensive gene flow an ... | 2017 | 27889871 |
developmental study of the proboscis extension response to heat in rhodnius prolixus along the life cycle. | triatominae are blood-sucking insects that localise their hosts using a range of sensory signals to find food, and among them, the heat emitted by the hosts. heat is one of the main short-range cues in vertebrate hosts, able to trigger alone the proboscis extension response (per) that precedes the bite. previous studies demonstrated that heat responsiveness of fifth-instar nymphs is maximum to moderate temperatures (30-35°c) compatible with those of their vertebrate host's body surface. this stu ... | 2017 | 27890693 |
untangling the transmission dynamics of primary and secondary vectors of trypanosoma cruzi in colombia: parasite infection, feeding sources and discrete typing units. | trypanosoma cruzi is the causative agent of chagas disease. due to its genetic diversity has been classified into six discrete typing units (dtus) in association with transmission cycles. in colombia, natural t. cruzi infection has been detected in 15 triatomine species. there is scarce information regarding the infection rates, dtus and feeding preferences of secondary vectors. therefore, the aim of this study was to determine t. cruzi infection rates, parasite dtu, ecotopes, insect stages, geo ... | 2016 | 27903288 |
effect of the saliva from different triatomine species on the biology and immunity of tlr-4 ligand and trypanosoma cruzi-stimulated dendritic cells. | triatomines are blood-sucking vectors of trypanosoma cruzi, the causative agent of chagas disease. during feeding, triatomines surpass the skin host response through biomolecules present in their saliva. dendritic cells (dcs) play a crucial role in the induction of the protection to aggressive agents, including blood-sucking arthropods. here, we evaluated if salivary components of triatomines from different genera evade the host immunity by modulating the biology and the function of lps- or t. c ... | 2016 | 27938380 |
triatomine bugs, their microbiota and trypanosoma cruzi: asymmetric responses of bacteria to an infected blood meal. | triatomine bugs (hemiptera: reduviidae) are vectors of the flagellate trypanosoma cruzi, the causative agent of chagas disease. the study of triatomine gut microbiota has gained relevance in the last years due to its possible role in vector competence and prospective use in control strategies. the objective of this study is to examine changes in the gut microbiota composition of triatomines in response to a t. cruzi-infected blood meal and identifying key factors determining those changes. | 2016 | 27938415 |
aggregated oviposition in rhodnius prolixus, sensory cues and physiological consequences. | females of the haematophagous bug rhodnius prolixus attach their eggs in clusters on substrates related to their hosts, such as nests or avian feathers. because the hosts are an enormous food resource as well as potential predators, the choice of the site and pattern of oviposition could have an important adaptive value. here we investigated proximate and a potential ultimate cause of this aggregated pattern of laid eggs. first, we studied proximal causes by analyzing the use of chemical or phys ... | 2017 | 27940266 |
deficiency of glycerol-3-phosphate acyltransferase 1 decreases triacylglycerol storage and induces fatty acid oxidation in insect fat body. | glycerol-3-phosphate acyltransferases (gpat) catalyze the initial and rate-limiting step for the de novo synthesis of triacylglycerol (tag). four mammalian gpat isoforms have been identified: the mitochondria-associated gpat1 and 2, and the endoplasmic reticulum (er)-associated gpat3 and 4. in the insect rhodnius prolixus, a vector of chagas' disease, we previously predicted a mitochondrial-like isoform (rhoprgpat1) from genomic data. in the current study, we clone the rhoprgpat1 coding sequence ... | 2017 | 27956137 |
salt controls feeding decisions in a blood-sucking insect. | salts are necessary for maintaining homeostatic conditions within the body of all living organisms. like with all essential nutrients, deficient or excessive ingestion of salts can result in adverse health effects. the taste system is a primary sensory modality that helps animals to make adequate feeding decisions in terms of salt consumption. in this work we show that sodium and potassium chloride salts modulate the feeding behavior of rhodnius prolixus in a concentration-dependent manner. feed ... | 2017 | 27989677 |
neuropeptidomics in triatoma infestans. comparative transcriptomic analysis among triatomines. | chagas' disease, affecting up to 6-7 million people worldwide, is transmitted to humans through the feces of triatomine kissing bugs. from these, rhodnius prolixus, triatoma dimidiata, triatoma infestans and triatoma pallidipennis are important vectors distributed throughout the latin american subcontinent. resistance to pyrethroids has been developed by some triatomine populations, especially t. infestans, obstructing their control. given their role in the regulation of physiological processes, ... | 2016 | 27993629 |
structure and postembryonic development of the intersegmental nodules in the non-muscular joints of the antennae in rhodnius prolixus. | the antennae of insecta consist of two basal segments and the distal annulated flagellum lacking intrinsic muscles. non-muscular joints are important to preserve the flexibility and structure of the long heteropteran antennae which bear an intersegmental nodule on each non-muscular joint. little is known about their properties or function. here we characterize the structure and postembryonic development of the non-muscular joints of rhodnius prolixus antennae. using scanning electron microscopy, ... | 2017 | 27998742 |
an insulin-like growth factor in rhodnius prolixus is involved in post-feeding nutrient balance and growth. | growth of organisms is modulated by the availability of nutrients and energy, and is mostly regulated by insulin-like growth factors (igfs) through the insulin signaling system. in insects, igfs produced by the fat body induce cell division during the molt cycle, regulate adult body size, and have metabolic effects. here, we describe an igf from the hematophagous hemipteran rhodnius prolixus and show its activity in regulating growth and metabolism in the post-feeding period during the fifth, an ... | 2016 | 28018164 |
cloning, localization, and physiological effects of sulfakinin in the kissing bug, rhodnius prolixus. | sulfakinins (sks) are a family of multifunctional neuropeptides that have been shown to have myotropic activity on muscles of the digestive system and to function as feeding satiety factors. here, we confirm via cloning the presence of two sulfakinins (rhopr-sk-1 and rhopr-sk-2) in rhodnius prolixus. reverse transcriptase quantitative pcr demonstrates that the rhopr-sk transcript is highly expressed in the central nervous system (cns) of unfed fifth-instar r. prolixus. fluorescent in situ hybrid ... | 2017 | 28024903 |
the molecular sensory machinery of a chagas disease vector: expression changes through imaginal moult and sexually dimorphic features. | the triatomine bug rhodnius prolixus is a main vector of chagas disease, which affects several million people, mostly in latin-america. host searching, pheromone communication, and microclimatic preferences are aspects of its behaviour that depend on multimodal sensory inputs. the molecular bases of these sensory processes are largely unknown. the expression levels of genes transcribed in antennae were compared between 5(th) instar larvae, and female and male adults by means of rna-seq. the ante ... | 2017 | 28059141 |
chromosome painting in triatomine insects reveals shared sequences between x chromosomes and autosomes. | in order to provide a broad picture on the origin and evolution of holocentric x chromosomes in heteropteran species, we prepared a sex chromosome painting probe by microdissection of the x1 and x2 chromosomes from a kissing bug mepraia spinolai (hemiptera: reduviidae: triatominae). fluorescence in situ hybridization on four species of the triatomini having different amounts of autosomal heterochromatin and sex chromosome systems show that the xs probe hybridizes on the euchromatin, located both ... | 2017 | 28082631 |
orcokinin neuropeptides regulate ecdysis in the hemimetabolous insect rhodnius prolixus. | to grow and develop insects must undergo ecdysis. during this process, the individual sheds the old cuticle to emerge as the following developmental stage. during ecdysis, different programed behaviors are regulated by neuropeptidergic pathways. in general, components of these pathways are better characterized in crustacean and holometabolous insects than in hemimetabola. in insects, the orkoninin gene produces two different neuropeptide precursors by alternative splicing: orcokinin a and orcoki ... | 2017 | 28089691 |
ecology of trypanosoma cruzi i genotypes across rhodnius prolixus captured in attalea butyracea palms. | trypanosoma cruzi, the agent of chagas disease exhibits significant genetic diversity. this parasite is divided into six discrete typing units (dtus) where t. cruzi i (tci) is the most widespread in the americas. tci genotypes have been associated to domestic and sylvatic cycles of transmission (tcidom and sylvatic tci). due to the importance of the enzootic transmission, we determined the frequency of tci genotypes present in rhodnius prolixus captured in different regions of the palm a. butyra ... | 2017 | 28104454 |
jaburetox affects gene expression and enzyme activities in rhodnius prolixus, a chagas' disease vector. | jaburetox, a recombinant peptide of ∼11kda derived from one of the canavalia ensiformis (jack bean) urease isoforms, is toxic and lethal to insects belonging to different orders when administered orally or via injection. previous findings indicated that jaburetox acts on insects in a complex fashion, inhibiting diuresis and the transmembrane potential of malpighian tubules, interfering with muscle contractility and affecting the immune system. in vitro, jaburetox forms ionic channels and alters ... | 2017 | 28108369 |
differences in competitive ability for the occupancy of shelters in triatomines. | triatomines (hemiptera: reduviidae: triatominae) are nocturnal blood-sucking insects. during daylight hours they remain in an akinetic state inside their shelters, whereas at dusk they become active and move outside. when they are outside their shelters during the photophase, triatomines are vulnerable to diurnal predators and the period just before dawn is critical to their survival. this work analyses the existence of competitive interactions involved in the occupancy of shelters by triatomine ... | 2017 | 28145576 |
comparative and functional triatomine genomics reveals reductions and expansions in insecticide resistance-related gene families. | triatomine insects are vectors of trypanosoma cruzi, a protozoan parasite that is the causative agent of chagas' disease. this is a neglected disease affecting approximately 8 million people in latin america. the existence of diverse pyrethroid resistant populations of at least two species demonstrates the potential of triatomines to develop high levels of insecticide resistance. therefore, the incorporation of strategies for resistance management is a main concern for vector control programs. t ... | 2017 | 28199333 |
octopamine and tyramine regulate the activity of reproductive visceral muscles in the adult female blood-feeding bug, rhodnius prolixus. | the role of octopamine and tyramine in regulating spontaneous contractions of reproductive tissues was examined in the female rhodnius prolixus octopamine decreased the amplitude of spontaneous contractions of the oviducts and reduced rhoprfirfa-induced contractions in a dose-dependent manner, whereas tyramine only reduced the rhoprfirfa-induced contractions. both octopamine and tyramine decreased the frequency of spontaneous bursal contractions and completely abolished the contractions at 5×10- ... | 2017 | 28235907 |
celebrating the sequencing of the rhodnius prolixus genome: a tribute to the memory of vincent b. wigglesworth. | 2018 | 28254111 | |
rhodnius prolixus: from classical physiology to modern developmental biology. | the hemiptera rhodnius prolixus is a blood-feeding insect and a primary vector of trypanosoma cruzi, the etiological agent of the chagas disease. over the past century, rhodnius has been the subject of intense investigations, which have contributed to unveil important aspects of metabolism and physiology in insects. recent technological innovations are helping dissect the genetic and molecular underpinnings of rhodnius embryogenesis and organogenesis, thus fostering the use of this important spe ... | 2017 | 28432816 |
developmental roles of tyrosine metabolism enzymes in the blood-sucking insect rhodnius prolixus. | the phenylalanine/tyrosine degradation pathway is frequently described as a catabolic pathway that funnels aromatic amino acids into citric acid cycle intermediates. previously, we demonstrated that the accumulation of tyrosine generated during the hydrolysis of blood meal proteins in rhodnius prolixus is potentially toxic, a harmful outcome that is prevented by the action of the first two enzymes in the tyrosine degradation pathway. in this work, we further evaluated the relevance of all other ... | 2017 | 28469016 |
[diversity of triatominae (hemiptera: reduviidae) in santander, colombia: epidemiological implications]. | domestic and wild triatomines in the department of santander have an epidemiological impact, as recently they have been linked to outbreaks of acute chagas disease. the analysis of their diversity and temporal variation contributes to the understanding of their biology and ecology in one of the most endemic areas of the country. | 2017 | 28527247 |
[possible oral transmission of chagas disease among hydrocarbons sector workers in casanare, colombia, 2014]. | trypanosoma cruzi, the etiological agent for chagas disease, can be transmitted by oral intake of contaminated food or drinks. during epidemiological week 14 of 2014, two cases of acute chagas disease were notified among hydrocarbons sector workers in paz de ariporo, casanare. | 2017 | 28527286 |
standardization of a continuous assay for glycosidases and its use for screening insect gut samples at individual and populational levels. | glycoside hydrolases (ghs) are enzymes able to recognize and cleave glycosidic bonds. insect ghs play decisive roles in digestion, in plant-herbivore, and host-pathogen interactions. gh activity is normally measured by the detection of a release from the substrate of products as sugars units, colored, or fluorescent groups. in most cases, the conditions for product release and detection differ, resulting in discontinuous assays. the current protocols result in using large amounts of reaction mix ... | 0 | 28553236 |
cytogenetic analysis in different populations of rhodnius prolixus and r. nasutus from different countries of south america. | 2017 | 28562785 | |
jaburetox-induced toxic effects on the hemocytes of rhodnius prolixus (hemiptera: reduviidae). | jaburetox is a recombinant peptide derived from a canavalia ensiformis urease that presents toxic effects upon several species of insects, phytopathogenic fungi and yeasts of medical importance. so far, no toxicity of jaburetox to mammals has been shown. previous reports have identified biochemical targets of this toxic peptide in insect models, although its mechanism of action is not completely understood. in this work, we aimed to characterize the effects of jaburetox in hemolymphatic insect c ... | 2017 | 28602911 |
proteomic analysis of the kissing bug rhodnius prolixus antenna. | reception of odorants is essential in insects' life since the chemical signals in the environment (=semiochemicals) convey information about availability of hosts for a blood meal, mates for reproduction, sites for oviposition and other relevant information for fitness in the environment. once they reach the antennae, these semiochemicals bind to odorant-binding proteins and are transported through the sensillar lymph until reach the odorant receptors. such perireceptor events, particularly the ... | 2017 | 28606853 |
behavioral evidence of oxidative stress by hematophagy in the ocellar system of rhodnius prolixus stål, 1859 red-eyed mutants. | the photonegative response to light stimulation in rhodnius prolixus (reduviidae, triatominae) is modulated by compound eyes and ocelli. screening pigments in the visual system have been shown to protect the cellular structures from the oxidative stress damage produced by blood ingestion and light stimulation. red-eyed mutants of rhodnius prolixus lack screening pigments in their compound eyes and ocelli and are exposed to more oxidative stress. experiments with rhodnius prolixus and triatoma in ... | 2017 | 28636880 |
catalase expression impairs oxidative stress-mediated signalling in trypanosoma cruzi. | trypanosoma cruzi is exposed to oxidative stresses during its life cycle, and amongst the strategies employed by this parasite to deal with these situations sits a peculiar trypanothione-dependent antioxidant system. remarkably, t. cruzi's antioxidant repertoire does not include catalase. in an attempt to shed light on what are the reasons by which this parasite lacks this enzyme, a t. cruzi cell line stably expressing catalase showed an increased resistance to hydrogen peroxide (h2o2) when comp ... | 2017 | 28653592 |
evolution and multiple roles of the pancrustacea specific transcription factor zelda in insects. | gene regulatory networks (grns) evolve as a result of the coevolutionary processes acting on transcription factors (tfs) and the cis-regulatory modules they bind. the zinc-finger tf zelda (zld) is essential for the maternal-to-zygotic transition (mzt) in drosophila melanogaster, where it directly binds over thousand cis-regulatory modules to regulate chromatin accessibility. d. melanogaster displays a long germ type of embryonic development, where all segments are simultaneously generated along ... | 2017 | 28671979 |
the involvement of rhopr-crf/dh in feeding and reproduction in the blood-gorging insect rhodnius prolixus. | rhodnius prolixus is a blood-gorging insect and a vector for human chagas disease. the insect transmits the disease following feeding, when it excretes urine and feces contaminated with the trypanosoma cruzi parasite. a corticotropin-releasing factor-like peptide acts as a diuretic hormone in r. prolixus (rhopr-crf/dh); however, its distribution throughout the insect's central nervous system (cns) and the expression of its receptor in feeding-related tissue as well as the female reproductive sys ... | 2017 | 28694056 |
comparative 2-d electrophoresis of salivary proteins in triatoma dimidiata and rhodnius prolixus (hemiptera: reduviidae) and major cross-reactive antigens | an immune response to triatomine’s saliva is an immunological marker of exposure to triatomine bites. however, considerable variability in salivary protein profiles did exist among species. in the present work, we compare salivary proteins from mexican triatoma dimidiata and rhodnius prolixus using 2-d electrophoresis. a clear differential saliva profile was found to exist between these two triatomine species. fewer protein spots were detected in r. prolixus than in t. dimidiata. more than half ... | 2017 | 28822204 |
[blood-feeding of rhodnius prolixus]. | triatomines (hemiptera: reduviidae) are blood-sucking insect vectors of the protozoan trypanosoma cruzi which is the causative agent of chagas' disease. rhodnius prolixus is the most epidemiologically important vector of t. cruzi in colombia. triatomines are regarded to be vessel-feeders as they obtain their blood meals from vertebrate hosts by directly inserting their mouthparts into vessels. microscopic techniques are useful for visualizing and describing the morphology of biological structure ... | 2017 | 28968005 |
cloning and functional characterization of octβ2-receptor and tyr1-receptor in the chagas disease vector, rhodnius prolixus. | octopamine and tyramine, both biogenic amines, are bioactive chemicals important in diverse physiological processes in invertebrates. in insects, octopamine and tyramine operate analogously to epinephrine and norepinephrine in the vertebrates. octopamine and tyramine bind to g-protein coupled receptors (gpcrs) leading to changes in second messenger levels and thereby modifying the function in target tissues and insect behavior. in this paper, we report the cdna sequences of two gpcrs, rhoproctβ2 ... | 2017 | 29018364 |
expression and functional characterization of tachykinin-related peptides in the blood-feeding bug, rhodnius prolixus. | tachykinins (tachykinin-related peptides, trps) are multifunctional neuropeptides that have widespread distribution in the central nervous system (cns) and in the gastrointestinal tract of many insects, and most have been shown to stimulate contractions of visceral muscles. invertebrate trps carry a characteristic conserved c-terminal pentapeptide (fxgxr-amide) and most of them share some amino acid sequence similarities (approx. 45%) with the vertebrate and mammalian tachykinin family. we have ... | 2018 | 29133203 |
nitric oxide contributes to high-salt perception in a blood-sucking insect model. | in all organisms, salts produce either appetitive or aversive responses depending on the concentration. while low-salt concentration in food elicits positive responses to ingest, high-salt triggers aversion. still the mechanisms involved in this dual behavior have just started to be uncovered in some organisms. in rhodnius prolixus, using pharmacological and behavioral assays, we demonstrated that upon high-salt detection in food a nitric oxide (no) dependent cascade is activated. this activatio ... | 2017 | 29138480 |
countercurrent heat exchange and thermoregulation during blood-feeding in kissing bugs. | blood-sucking insects experience thermal stress at each feeding event on endothermic vertebrates. we used thermography to examine how kissing-bugs rhodnius prolixus actively protect themselves from overheating. during feeding, these bugs sequester and dissipate the excess heat in their heads while maintaining an abdominal temperature close to ambient. we employed a functional-morphological approach, combining histology, µct and x-ray-synchrotron imaging to shed light on the way these insects man ... | 2017 | 29157359 |
bugs battle stress from hot blood. | a heat exchange mechanism in the head of kissing bugs helps to prevent stress and regulate their temperature while they feed on warm blood. | 2017 | 29157360 |
[insecticidal action of synthetic girgensohnine analogues and essential oils on rhodnius prolixus (hemiptera: reduviidae)]. | the alkaloid girgensohnine has been used as a natural model in the synthesis of new alkaloid-like alpha-aminonitriles with insecticidal effect against disease vectors. | 2017 | 29161477 |
functional aspects of salivary nitric oxide synthase of rhodnius prolixus (hemiptera, reduviidae) and nitric oxide trafficking at the vector-host interface. | rhodnius prolixus expresses nitric oxide synthase (nos) in the cytosol of the salivary gland (sg) cells. the no produced is stored in the sg lumen bound to no-carrier haemeproteins called nitrophorins (nps). nps bind tightly to no in the acidic sg lumen, but release no when the ph becomes high, e.g., at the host skin (ph~7.4). no elicits potent and transient relaxation of vascular smooth muscle. here, we investigated the role of salivary no in the r. prolixus feeding behaviour and the salivary v ... | 2017 | 29167493 |
clock gene period in the chagas disease vector triatoma infestans (hemiptera: reduviidae). | to contribute to a better understanding of the molecular bases of the circadian biological rhythms in chagas disease vectors, in this work we identified functional domains in the sequences of the clock protein period (per) in rhodnius prolixus and triatoma infestans and analyzed the expression of the per gene at mrna level in t. infestans. the per protein sequences comparison among these species and those from other insects revealed that the most similar regions are the pas domains and the most ... | 2017 | 29260645 |
genome of russian wheat aphid an economically important cereal aphid. | although the hemipterans (aphididae) are comprised of roughly 50,000 extant insect species, only four have sequenced genomes that are publically available, namely acyrthosiphon pisum (pea aphid), rhodnius prolixus (kissing bug), myzus persicae (green peach aphid) and diuraphis noxia (russian wheat aphid). as a significant proportion of agricultural pests are phloem feeding aphids, it is crucial for sustained global food security that a greater understanding of the genomic and molecular functioni ... | 2017 | 29299110 |
pattern and composition of ionic currents around ovarioles of the hemipteran, rhodnius prolixus (stahl). | two-dimensional vibrating probe analysis of extracellular currents around rhodnius prolixus ovarioles correlated with an earlier one-dimensional analysis but revealed asymmetrical circumferential current patterns and large tangential currents. na+, k+, and ca+2 have been tentatively identified as the major ions involved in these currents, with na+ being the major ion involved in current efflux. | 1989 | 29300571 |
genome wide mapping of peptidases in rhodnius prolixus: identification of protease gene duplications, horizontally transferred proteases and analysis of peptidase a1 structures, with considerations on their role in the evolution of hematophagy in triatominae. | triatominae is a subfamily of the order hemiptera whose species are able to feed in the vertebrate blood (i.e., hematophagy). this feeding behavior presents a great physiological challenge to insects, especially in hemipteran species with a digestion performed by lysosomal-like cathepsins instead of the more common trypsin-like enzymes. with the aim of having a deeper understanding of protease involvement in the evolutionary adaptation for hematophagy in hemipterans, we screened peptidases in th ... | 2017 | 29326597 |
comparative genomics analysis of triatomines reveals common first line and inducible immunity-related genes and the absence of imd canonical components among hemimetabolous arthropods. | insects operate complex humoral and cellular immune strategies to fend against invading microorganisms. the majority of these have been characterized in drosophila and other dipterans. information on hemipterans, including triatominae vectors of chagas disease remains incomplete and fractionated. | 2018 | 29357911 |
cdna isolation and expression of nicotinamide adenine dinucleotide phosphate-dependent cytochrome p450 reductase gene in the chagas disease vector triatoma infestans. | pyrethroid resistance has been detected in triatoma infestans (hemiptera: reduviidae), which was atributed to target site insensitivity and increased oxidative metabolism of the insecticide by cytochrome p450s. nicotinamide adenine dinucleotide phosphate (nadph) cytochrome p450 reductase (cpr) plays an essential role in transferring electrons from nadph to the p450-substrate complex. in this study, the full length cpr cdna of t. infestans was isolated and gene expression was determined by quanti ... | 2018 | 29363459 |