Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| acute hemorrhagic conjunctivitis due to enterovirus 70 in american samoa: serum-neutralizing antibodies and sex-specific protection. | acute hemorrhagic conjunctivitis due to enterovirus 70 has caused extensive outbreaks in tropical areas since 1969. between december 1, 1990, and march 4, 1991, an outbreak of acute hemorrhagic conjunctivitis due to enterovirus 70 occurred in american samoa, where an outbreak due to the same agent had occurred in 1981. a survey of 5% of the households (134 households, 1,095 individuals) was conducted throughout the island of tutuila. the outbreak affected 58% of the population, with age-specific ... | 1992 | 1337662 |
| antibody responses to avian encephalomyelitis virus vaccines when administered by different routes. | antibody responses to a commercial avian encephalomyelitis virus (aev) vaccine administered by different routes were measured by an enzyme-linked immunosorbent assay (elisa). responses to single doses of vaccine administered by the ocular route to 10% of a flock were comparable with those obtained when all birds received a single dose in the drinking water. however, ocular vaccination of 5% of the flock resulted in significantly lower responses than those obtained when 10% were vaccinated. mater ... | 1992 | 1337695 |
| long-term observation of neutralization antibody after enterovirus 70 infection. | there is the need for more information on the persistence of the immune response to enterovirus 70 (ev70) infection. in august 1984, an outbreak of acute hemorrhagic conjunctivitis occurred at a nursing home in sapporo, japan. during the subsequent 7 years, we have monitored the neutralization antibody of ev70 in 13 patients with acute hemorrhagic conjunctivitis and 15 persons with subclinical ev70 infection among the 108 persons in the nursing home. during the first one to two years, the neutra ... | 1992 | 1337761 |
| effect of verapamil on acute coxsackievirus b3 murine myocarditis. | the effect of verapamil (ver) on cvb3 murine myocarditis was investigated. it was found that ver could aggravate the myocardial inflammation, increase the viral replication in myocardium, and raise mortality in mice with viral myocarditis when the drug was injected within the first 6 days after the cvb3 inoculation. | 1992 | 1337875 |
| an epidemic of acute haemorrhagic conjunctivitis caused by coxsackie a24 variant. | an epidemic of acute haemorrhagic conjunctivitis (ahc) caused by a variant of coxsackie a24 (cox a24) occurred in delhi during august to september 1988. cox a24 antigen was detected by indirect immunofluorescence (ifa) in conjunctival cell smears of 13 of the 38 (34.2%) patients studied. virus was isolated from conjunctival swabs in 11 (28.9%) patients and all isolates were neutralized by cox a24 antiserum. five virus strains sent to virology division of centres for disease control, atlanta, usa ... | 1992 | 1337897 |
| enteroviral aseptic meningitis in japan, 1981-1991. a report of the national epidemiological surveillance of infectious agents in japan. | in japan, aseptic meningitis cases due to enterovirus infections increase every summer in various degrees with an incidence peak usually in july. during the past 11 years from 1981 through 1991, a total of 8,595 enterovirus isolations from aseptic meningitis cases were reported from 54 participating laboratories. eight enterovirus types caused large epidemics; more than 100 isolations of each type from aseptic meningitis cases were reported for every epidemic year of the respective type. they we ... | 1992 | 1337925 |
| [the relationship of clinical manifestations and age distribution in enterovirus infections--viral isolation and seroepidemiology in aichi prefecture]. | we studied the epidemiology of enterovirus infection in aichi prefecture from 1985 to 1989. we examined the age distribution of aseptic meningitis patients (am) and exanthematous disease patients (ex) and a seroepidemiological study of echovirus type 7 (e7), e9, e18 and group a coxsackievirus type 9 (ca9), was performed. the results is as follows: 1) e7, e9, e18 and ca9 were isolated from am and ex but e6, e11 and group b coxsackie viruses (cb) were isolated in fewer cases from ex. 2) the am was ... | 1992 | 1338208 |
| [live enteroviral vaccines for the emergency nonspecific prevention of mass respiratory diseases during fall-winter epidemics of influenza and acute respiratory diseases]. | the results of the 3-year controlled trials of a new method of nonspecific urgent prophylaxis of influenza and acute respiratory diseases (adr) by immunization of healthy adults with standard live enterovirus oral vaccines, introduced in 2-3 administrations at intervals of 7-10 days, at the initial stages of autumn and winter epidemics are presented. observations, carried out in three republics, covered more than 150,000 persons immunized with enterovirus interferonogenic vaccines. a considerabl ... | 1992 | 1338742 |
| [serological study of the incidence of murine viruses in a population of small wild rodents (microtus pennsylvanicus ord, 1815)]. | the results of a serological survey of a free-living population of meadow voles (microtus pennsylvanicus) in pinawa, manitoba (canada) showed that these animals possessed antibodies to six of the eleven viruses tested for, namely: reovirus type 3, murine encephalomyelitis agent, ectromelia virus, murine adenovirus, murine hepatitis virus and lymphocytic choriomeningitis virus. the significant increase in the number of individuals possessing specific antibodies suggests that these viruses, or rel ... | 1992 | 1339064 |
| rapid detection of waterborne viruses using the polymerase chain reaction and a gene probe. | we describe a membrane-filter-based urea-arginine phosphate buffer method for concentrating waterborne viruses from large volumes of water to microlitre volumes, and their subsequent detection by the polymerase chain reaction (pcr). the detection step involves the extraction of rna, synthesis of complementary dna, amplification by pcr of target dna with specific primers, and confirmation through nucleic acid hybridization with a radiolabelled oligonucleotide probe. the pcr technique detected the ... | 1992 | 1339185 |
| evaluation of indirect enzyme linked immunosorbent assay for the detection of coxsackieviruses in clinical samples and its comparison with dot-immunobinding assay. | indirect enzyme linked immunosorbent assay (elisa) was applied for the direct detection of coxsackieviruses in clinical samples viz. rectal swabs (rs) and throat swabs (ts) collected from patients admitted to various nursing homes and local hospitals. results indicate the presence of different cva types in 65 (62.5%) out of 104 rs, and 18 (52.9%) out of 34 ts samples. dot-immunobinding assay was also standardized for the identification of cva types employing 52 rs samples and the results compare ... | 1992 | 1343943 |
| high frequency of igm antibodies to coxsackie b virus in sarcoidosis patients and patients with asbestos-related lesions. | by using radioimmunoassay (ria) for detection of igm antibodies to coxsackie b viruses (cbv), the occurrence of these antibodies was investigated in patients with sarcoidosis and asbestos-related lesions. sixty-one per cent of the patients with sarcoidosis, all patients with benign asbestos pleural effusion, and 67% of those with diffuse asbestos-related pleural thickening showed cbv-igm. patients with healed sarcoidosis or pleural plaques were all negative, and among the "healthy" controls seve ... | 1992 | 1344042 |
| polyclonal immunoglobulin therapy protects against cardiac damage in experimental coxsackievirus-induced myocarditis. | balb/c male mice infected i.p. with 2 x 10(5) plaque forming units (pfu) of coxsackievirus b3 (cvb3) develop severe myocarditis 7 days later. studies were performed to determine whether therapy with normal polyclonal immunoglobulin would prevent cardiac inflammation. partially purified immunoglobulin was derived from pooled mouse serum by ammonium sulphate precipitation. infected animals given either 100 or 1000 micrograms of this preparation for 2 days prior to infection showed greater than 50% ... | 1992 | 1349527 |
| lessons from animal experiments in myocarditis. | we have developed murine models of viral myocarditis induced by encephalomyocarditis (emc) virus in which severe myocarditis, congestive heart failure and dilated cardiomyopathy occur in high incidence. from these models, we have learned the natural history and pathogenesis and assessed new diagnostic methods and therapeutic and preventive interventions. mural thrombi in the atria and ventricles, ventricular aneurysms, conduction disturbance and various arrhythmias were seen in these models. ant ... | 1992 | 1349548 |
| viruses in idiopathic inflammatory myopathies: absence of candidate viral genomes in muscle. | there is indirect evidence that various viruses have aetiological roles in the idiopathic inflammatory myopathies. by means of a sensitive and specific method based on the polymerase chain reaction (pcr), we sought direct evidence for the presence in affected muscle of nucleic acid sequences from coxsackie virus, mumps virus, encephalomyocarditis virus, adenovirus, human t-lymphotropic virus types i and ii, and human immunodeficiency virus. rna was extracted from muscle biopsy samples obtained f ... | 1992 | 1349938 |
| igg subclass responses to theiler's murine encephalomyelitis virus infection and immunization suggest a dominant role for th1 cells in susceptible mouse strains. | inbred mouse strains differ in susceptibility to theiler's murine encephalomyelitis virus (tmev)-induced demyelinating disease. a strong correlation between disease susceptibility and delayed-type hypersensitivity (dth) has been previously demonstrated, but no strong correlation between disease susceptibility and total anti-tmev elisa titres was shown. since both dth and igg2a antibody production are regulated by cd4+ th1 cells, we investigated three strains of mice to determine whether antiviru ... | 1992 | 1350571 |
| common aetiological agent for epidemic and sporadic non-a, non-b hepatitis. | enterovirus-like particles have been reported in the acute phase of both epidemic and sporadic non-a, non-b (nanb) hepatitis. to examine whether these particles were the causative agent in the two types of disease, 29 patients with acute viral hepatitis in a north indian epidemic outbreak and 9 with sporadic acute disease were investigated. 25 (86%) of 29 patients with epidemic hepatitis and 5 (56%) of 9 with sporadic disease were diagnosed as having enterically-transmitted-nanb hepatitis by exc ... | 1992 | 1351189 |
| health effects of white-water canoeing. | there is little quantitative information on the relation between water quality and disease attack rates after recreational activities in fresh water. we conducted a prospective cohort study to measure the health effects of white-water and slalom canoeing in two channels with different degrees of microbial contamination. site a, fed by a lowland river, showed high enterovirus concentrations (arithmetic mean 198 pfu per 10 litre and moderate faecal coliform concentrations (geometric mean 285/dl); ... | 1992 | 1351560 |
| detection of enterovirus rna in patients with idiopathic dilated cardiomyopathy by polymerase chain reaction. | the pathogenic role of enterovirus in patients with idiopathic dilated cardiomyopathy has been determined through a molecular biologic approach. sensitivity in the detection of viral genomes in tissues varied between conventional nucleic acid hybridization and polymerase chain gene amplification. to improve diagnosis, we developed a strategy for reverse transcription polymerase chain reaction (rt-pcr) to detect viral rna. we synthesized two sequence-specific oligonucleotides, primer 1 (5'daccgac ... | 1992 | 1358340 |
| the effect of alpha 1-blocker, bunazosin on a murine model of congestive heart failure induced by viral myocarditis. | the purpose of this study was to investigate the therapeutic effect of an alpha 1-blocker, bunazosin, using an experimental murine model of congestive heart failure induced by viral myocarditis. this model is characterized by a high incidence of severe myocarditis and subsequent congestive heart failure, and is suitable for the evaluation of the effect of drugs. to estimate myocardial damage objectively and quantitatively, we used antimyosin monoclonal antibody in addition to histopathological g ... | 1992 | 1360548 |
| effect of enterovirus infection on susceptibility of hela cells to shigella flexneri invasivity. | invasiveness of shigella flexneri m90t in hela cells was significantly increased when cells were preinfected with poliovirus 1, coxsackievirus b3 and echovirus 6. this effect was dependent on the dose of virus used, evident at early stages of viral infection and lasted hours before the appearance of a cytopathic effect. an increase of bacterial invasion ability was also noticed when hela cells were incubated with uv-inactivated enteroviruses. this enhancing effect obtained with both viable and u ... | 1992 | 1364020 |
| a method for the preparation of purified antigens of coxsackievirus b3 from a large volume of cell culture supernatant. | a simple procedure was used for the concentration and partial purification of coxsackievirus b3 (nancy strain). for a large-scale production of virus. vero cells grown in roller bottles were used. virus concentrate from a large volume of cell culture supernatant was prepared by precipitation with 6% (w/w) polyethylene glycol. this crude antigen was further purified by banding in cesium chloride gradient using ultracentrifugation. the infectivity and haemagglutination activity of virus were check ... | 1992 | 1364026 |
| expression and purification of recombinant 3c proteinase of coxsackievirus b3. | we have cloned various lengths of coxsackievirus b3 cdna encompassing the region encoding the 3c proteinase, which is essential to the viral replication cycle. such viral cdnas were fused in frame to the 5'terminal portion of the lacz' gene carried on the vector puc118 to express mature 3c proteinase in escherichia coli. in the e. coli cells containing pcxb108 or pcxb117, constructed for this study, a large amount of 23-kda protein was synthesized in the presence of iptg. this protein was purifi ... | 1992 | 1369382 |
| comoviruses and enteroviruses share a t cell epitope. | an in vitro murine t cell proliferation assay was used to determine whether an antigenic epitope(s) recognized by enterovirus-immune t cells is held in common between plant comoviruses and human enteroviruses. splenocytes isolated from c3h/hej mice infected with coxsackievirus b3 (cvb3) proliferated in vitro not only against a variety of enterovirus (cvb2, cvb3, cvb6, cva16, pv1) antigens, but against comovirus (cpmv, bpmv) antigens as well. splenocytes from mice inoculated with bean pod mottle ... | 1992 | 1370127 |
| autoimmunity to two forms of glutamate decarboxylase in insulin-dependent diabetes mellitus. | insulin-dependent diabetes mellitus (iddm) is thought to result from the autoimmune destruction of the insulin-producing beta cells of the pancreas. years before iddm symptoms appear, we can detect autoantibodies to one or both forms of glutamate decarboxylase (gad65 and gad67), synthesized from their respective cdnas in a bacterial expression system. individual iddm sera show distinctive profiles of epitope recognition, suggesting different humoral immune responses. although the level of gad au ... | 1992 | 1370298 |
| general primer-mediated polymerase chain reaction for detection of enteroviruses: application for diagnostic routine and persistent infections. | the aim of this study was to determine the applicability of the polymerase chain reaction (pcr) for routine diagnostic use and for the detection of persistent enteroviral infections. to this end, general primers were selected in the highly conserved part of the 5'-noncoding region of the enteroviral genome. they were tested on 66 different enterovirus serotypes. a specific fragment was amplified from 60 of 66 serotypes. an amplification product was not observed from coxsackievirus types a11, a17 ... | 1992 | 1370845 |
| detection of enteroviruses in cell cultures by using in situ transcription. | in situ transcription (ist) was shown to be useful for the detection of human enteroviral rna in cultured cells. a primer to detect a wide variety of enteroviral genomes and a coxsackievirus type b3 genome-specific primer were demonstrated to be efficient in ist assays. transcription times greater than 10 to 30 min did not significantly improve the acquisition of a specific signal, whereas the signal-to-noise ratio decreased with time. inclusion of actinomycin d to suppress dna-dependent dna pol ... | 1992 | 1370849 |
| immune responses in the post-polio syndrome. | 1992 | 1370857 | |
| identification and localization of a limited number of predominant conformation-independent antibody epitopes of theiler's murine encephalomyelitus virus. | theiler's murine encephalomyelitis virus (tmev)-induced demyelinating disease in mice is a well established animal model for human multiple sclerosis (ms). identification of pathogenic epitopes may be helpful in understanding the pathogenesis of this immune-mediated disease. in order to analyze the viral epitopes, we have generated approx. 150 recombinant lambda gt11 clones expressing various capsid areas of tmev. six predominant areas, ranging from 13-26 amino acid residues, (3 in vp1, 2 in vp2 ... | 1992 | 1371268 |
| the effects of fk-506, a novel and potent immunosuppressant, upon murine coxsackievirus b3 myocarditis. | to test the therapeutic efficacy of immunosuppression with fk-506 upon coxsackievirus b3 myocarditis, c3h/he mice were inoculated with coxsackievirus b3, and the effects of fk-506 were compared to those of cyclosporine. fk-506 (2.5 mg/kg/day) or cyclosporine (25 mg/kg/day) was administered s.c. daily on days 0 to 14 (experiment i) and on days 14 to 28 (experiment ii). in experiment i, the survival rate of the fk-506 or cyclosporine-treated group was significantly lower compared with that of the ... | 1992 | 1372051 |
| use of non-neutralizing monoclonal antibodies in an elisa for intratypic differentiation of 28 echovirus type 25 clinical isolates. | three non-neutralizing monoclonal antibodies were produced and selected against echovirus type 25 jv-4 prototype strain. they were used in an elisa to investigate the intratypic differentiation of 28 wild isolates. clinical isolates fell into seven different groups according to their reactivity patterns in elisa. two of the non-neutralizing monoclonal antibodies, 9e4 and 6d3, were highly specific, while the third, 6c9, may recognize an epitope common to other types of echoviruses. in contrast, m ... | 1992 | 1372613 |
| interesting problems in enteroviral inflammatory heart disease. | enteroviral inflammatory heart disease is an established entity. based upon the accumulated work from many laboratories, specific and definitive questions can be posed regarding the pathogenic nature of the viruses involved and how the host immune systems respond to the insult of enterovirus replication in the myocardium. the answers to these questions may well lead to efficient intervention therapies such as vaccination or effective anti-viral compounds, thus eradicating enterovirus-induced hea ... | 1992 | 1374357 |
| 'run-off' polymerization with digoxigenin labelled nucleotides creates highly sensitive and strand specific dna hybridization probes: synthesis and application. | in this paper the in vitro synthesis and application of non-radioactively labelled strand specific dna probes is described. the probe is labelled by incorporation of nucleotides with the hapten digoxigenin into single-stranded dna during a 'run-off' reaction catalyzed by thermus aquaticus (taq) dna-polymerase. the 'run-off' reaction requires a linearized plasmid template and one primer binding site at a defined distance from the restriction site. single-stranded dna can be synthesized during rep ... | 1992 | 1381047 |
| characterization of neutralizing antibodies to bovine enterovirus elicited by synthetic peptides. | six synthetic peptides corresponding to regions of bovine enterovirus (bev), strain vg-5-27, elicited antibodies in mice which reacted with the virus in various assays. these antibodies have been characterised on the basis of their ability to (1) neutralize the virus, (2) bind to the intact virus particle in an immunoprecipitation test, (3) react with the denatured viral proteins, and (4) give immunofluorescent staining of virus infected cells. we have also determined the proportion of antipepti ... | 1992 | 1381910 |
| monoclonal anti-idiotypes induce neutralizing antibodies to enterovirus 70 conformational epitopes. | monoclonal antibodies (mabs) directed against the prototype enterovirus 70 (ev-70) strain j670/71 were generated and characterized in order to produce anti-idiotypic mabs (mab2s) for use as surrogate immunogens. western immunoblot and radioimmunoprecipitation assays suggested that all the mabs recognize conformational epitopes on the virion surface. an ev-70-neutralizing antibody, mab/ev-12 (mab1), was selected for the production of mab2s. five mab2s were selected for their capacities to inhibit ... | 1992 | 1382141 |
| viruses and rheumatic disease. | 1992 | 1393383 | |
| use of modified nucleotides and uracil-dna glycosylase (ung) for the control of contamination in the pcr-based amplification of rna. | the inadvertent carryover of amplified fragments of nucleic acids (amplicons) is a potential source of contamination in the polymerase chain reaction. recently, a method has been developed to generate amplicons with deoxyuracil triphosphate (dutp) and to specifically hydrolyze these amplicons with uracil-dna glycosylase (ung) following the completion of the assay. we evaluated this system for the specific amplification of rna from coxsackievirus a3 and b3. we found that rna from both viruses cou ... | 1992 | 1406734 |
| [epidemic outbreak of viral meningitis caused by type 30 echo virus]. | the clinical records of 15 children admitted to hospital infantile de méxico federico gómez with diagnosis of viral meningitis were reviewed. they were part of 19 patients admitted with this diagnosis during a 5 week period (march 22 to april 30, 1992) and represent a significant increase of aseptic meningitis compared with the same periods of previous years at hospital infantile de mexico and in mexico city where there is an ongoing epidemic outbreak of this entity. all the patients studied had ... | 1992 | 1418647 |
| symptomatology of children in contact with sea water contaminated with sewage. | the aim was to determine whether or not there was a measurable risk of ill health associated with contact with sea water for children between the ages of 6 and 11 years old. | 1992 | 1431703 |
| coexpression of class i major histocompatibility antigen and viral rna in central nervous system of mice infected with theiler's virus: a model for multiple sclerosis. | chronic infection of susceptible strains of mice with theiler's murine encephalomyelitis virus (tmev) results in central nervous system (cns) demyelination similar to multiple sclerosis. demyelination induced by tmev is mediated, in part, by class i-restricted cd8+ t lymphocytes. for these t cells to function, they must recognize virus-infected cns targets in the presence of class i major histocompatibility complex (mhc) antigen. therefore, we studied in vivo expression of class i mhc antigen an ... | 1992 | 1434926 |
| virus-induced demyelination: from animal models to human diseases. | 1992 | 1434936 | |
| no serological evidence for the presence of swine vesicular disease virus in south africa. | an indirect elisa incorporating a protein a-peroxidase conjugate was developed for detecting antibodies to swine vesicular disease virus (svdv) in pig sera. this test and a conventional virus neutralization test were found to be equally sensitive. a total of 2846 pig sera collected from various abattoirs in south africa were tested using the indirect elisa. no serological evidence of infection with svdv in pigs in south africa was found. | 1992 | 1437026 |
| multiple sclerosis and its animal models: the role of the major histocompatibility complex and the t cell receptor repertoire. | 1992 | 1440199 | |
| enteroviral meningitis in infants. | 1992 | 1454450 | |
| androstenediol regulates systemic resistance against lethal infections in mice. | we previously reported that subcutaneous injection of dhea (5-androsten-3 beta-ol-17-one, dehydroepiandrosterone) protected mice from lethal infection. this included both a lethal herpes virus type 2 encephalitis and a lethal systemic coxsackievirus b4 (cb4) infection. androstenediol (5-androsten-3 beta-17 beta-diol, aed), a metabolic product of dhea is up to 100 x more effective in regulating systemic resistance against lethal infection with cb 4 than its precursor dhea. compared to dhea, treat ... | 1992 | 1456887 |
| evidence for t-cell involvement during the acute phase of echovirus meningitis. | little is known about the cellular immune response during the acute phase of enterovirus infection. this was studied in patients with echovirus meningitis by analysing changes in the lymphocyte subset distribution both in blood and in cerebrospinal fluid (csf) and their stage of activation. a significant increase of whole t-cell and cd4+ t-cell counts was observed in csf in parallel with a slight decrease of t-cell percentage in blood. activation of the cellular immune response is supported by t ... | 1992 | 1460459 |
| [cerebrospinal meningitis and encephalitis--1990. 1. incidence and prevalence]. | 1992 | 1475395 | |
| pathologic and serologic characterization of a variant of duck hepatitis type i virus. | a duck hepatitis virus (dhv), isolated from ducks on a farm in virginia in 1963, was shown to be only partially related to dhv type i (dhv-i) in cross-neutralization and in cross-protection tests. the virus, named dhv-ia, apparently is a serologic variant of dhv-i; both viruses are serologically distinct from dhv type iii. pathologic responses to dhv-ia were similar to those described for dhv-i infection. | 1992 | 1485876 |
| disturbance of mouse pancreatic beta-cell function following echo 4 virus infection. | 1992 | 1486985 | |
| intrauterine fetal death due to echovirus 11. | in a case of intrauterine fetal death in the 29th week of gestation, echovirus 11 could be isolated from the umbilical cord of the fetus. the mother had no apparent signs of infection but serological evidence of current echovirus 11 infection. enterovirus pcr performed on paraffin-embedded specimens of various tissues (myocardium, lung, liver and placenta) from the fetus yielded positive results in all cases. these findings, together with supporting serological and epidemiological findings--e.g. ... | 1992 | 1509244 |
| acute haemorrhagic conjunctivitis outbreak. | 1992 | 1519148 | |
| a distinct picornavirus group identified by sequence analysis. | although echovirus 22 is presently classified as a member of the enterovirus group in the family of picornaviruses, it has been reported to have exceptional biological properties when compared with other representatives of the group. we have determined the complete nucleotide sequence of the echovirus 22 (harris strain) genome, which appears to be significantly different from all the other studied picornaviruses. however, the organization of the genome [7339 nucleotides, excluding the poly(a) tr ... | 1992 | 1528901 |
| [type 4 echovirus meningitis in childhood. epidemiologic and clinical aspects apropos of an epidemiologic outbreak]. | in the first half of 1988 we observed 13 cases of lymphocytic meningitis from which echovirus type 4 was isolated in the csf. we found a higher infection rate in the 6-9 years old group (54%) and in the masculine sex (69%). almost all of the cases occurred during the springtime. the clinical and csf characteristics of this epidemic do not differ much from those that have appeared in the literature. throughout 1988, in our hospital, 46 cases of lymphocytic meningitis took place and in 20 of them ... | 1992 | 1543290 |
| the changing spectrum of neonatal meningitis over a fifteen-year period. | one hundred seventy-seven cases of neonatal meningitis treated at the university of texas medical branch at galveston over a 15-year period (1974-1988) were reviewed. over this period, the incidence of bacterial meningitis decreased, the incidence of aseptic meningitis remained stable, and the diagnosis of enteroviral meningitis increased in frequency. during 1984-1988, enterovirus was the most common cause of meningitis in neonates older than seven days and accounted for one third of all cases ... | 1992 | 1547583 |
| pyrazole-related nucleosides. synthesis and antiviral/antitumor activity of some substituted pyrazole and pyrazolo[4,3-d]-1,2,3-triazin-4-one nucleosides. | several pyrazole and pyrazolo[4,3-d]-1,2,3-triazin-4-one ribonucleosides were prepared and tested for antiviral/antitumor activities. appropriate heterocyclic bases were prepared by standard methodologies. glycosylation of pyrazoles 6a-e,g,i and of pyrazolo[4,3-d]-1,2,3-triazin-4-ones 12f-1 mediated by silylation with hexamethyldisilazane, with 1-beta-o-acetyl-2,3,5-tri-o-benzoyl-d-ribofuranose, gave in good yields the corresponding glycosides 7a-e,g, 8g,i, 13f,h,k, and 14f, but could not be app ... | 1992 | 1548681 |
| a single base deletion in the 5' noncoding region of theiler's virus attenuates neurovirulence. | viral chimeras have been constructed through in vitro manipulations of the infectious cdna clones of two prototypes of theiler's murine encephalomyelitis virus: (i) the virulent gdvii strain and (ii) the less virulent bean and vl strains. previous studies have suggested that the phenotypic differences in virulence between the bean and gdvii strains map to both the 5' noncoding and the coat protein regions of these viral genomes. it is shown here that attenuation mapped to the 5' noncoding region ... | 1992 | 1548749 |
| finding out how a viral hitchhiker snags a ride. | 1992 | 1553554 | |
| identification of the integrin vla-2 as a receptor for echovirus 1. | cell surface receptors for echovirus, a common human pathogen, were identified with monoclonal antibodies that protected susceptible cells from infection with echovirus 1. these monoclonal antibodies, which prevented virus attachment to specific receptor sites, recognized the alpha and beta subunits of the integrin vla-2 (alpha 2 beta 1), a receptor for collagen and laminin. rd rhabdomyosarcoma cells expressed little vla-2, did not bind to 35s-labeled virus, and resisted infection until transfec ... | 1992 | 1553561 |
| theiler's virus replication in brain macrophages cultured in vitro. | infection of the mouse with theiler's virus is one of the best animal models for the study of multiple sclerosis, a chronic demyelinating disease of the human central nervous system. the identification of the virus target cell(s) is fundamental to an understanding of the viral persistence as well as the inflammation and demyelination observed in the chronic phase of the disease. this paper reports that a small fraction of brain macrophages grown in vitro can be efficiently infected with theiler' ... | 1992 | 1560544 |
| conservation of rna-protein interactions among picornaviruses. | picornavirus genomes encode unique 5' noncoding regions (5' ncrs) which are approximately 600 to 1,300 nucleotides in length, contain multiple upstream aug codons, and display the ability to form extensive secondary structures. a number of recent reports have shown that picornavirus 5' ncrs are able to facilitate cap-independent internal initiation of translation. this mechanism of translation occurs in the absence of viral gene products, suggesting that the host cell contains the necessary comp ... | 1992 | 1602550 |
| a 44,000 glycoprotein is involved in the attachment of echovirus-11 onto susceptible cells. | cellular receptors play an important role in viral pathogenesis. until now little was known on echovirus (ev) receptor. using detergent-treated kb cell extracts as immunogen, a mouse monoclonal antibody (mab 143) was produced that selectively blocks the attachment of ev-11 to kb and other susceptible cells. by immunoblotting, mab 143 detected a 44,000 protein on susceptible cell lines but not on cell lines from nonprimate origin. the receptor protein complex, purified from kb cell membranes by i ... | 1992 | 1604819 |
| replication of enteroviruses in human mononuclear cells. | 1992 | 1607276 | |
| coxsackievirus b4 infection of the mouse pancreas: the role of natural killer cells in the control of virus replication and resistance to infection. | the role of natural killer (nk) cells in the early immune response to a pancreatropic isolate of coxsackievirus b4 (cvb4) was investigated in a murine model of pancreatitis. endogenous (background) nk cell activity in fresh spleen effector cells from eight mouse strains was compared with virus-augmented nk cell activity 4 days post-infection (p.i.). a significant virus-induced increase (p less than or equal to 0.003) in nk cell activity was seen in seven of eight infected mouse strains, when vir ... | 1992 | 1607859 |
| coxsackievirus b4 infection of the mouse pancreas: acute and persistent infection. | the course of infection of a pancreas-adapted isolate of coxsackievirus b4 was followed over a 10 month period in a murine model. following intraperitoneal inoculation a typical acute infection was seen in nine of 10 inbred mouse strains. virus rapidly infected the exocrine pancreas, titres peaking 3 to 4 days post-infection (p.i.). lesions were almost exclusively confined to pancreatic acinar cells and varied in severity among the inbred strains. virus shed into the blood-stream was not cell-as ... | 1992 | 1607860 |
| chronic fatigue syndrome. | the chronic fatigue syndrome (cfs) is a poorly understood condition with nonspecific signs and symptoms, especially debilitating fatigue. most patients can pinpoint the onset of their illness and usually describe a flu-like state. the search for an etiologic agent has focused on a number of viruses such as epstein-barr, enteroviruses, retroviruses, and human herpesvirus-6. evidence supports persistent viral infection in a small percentage of cfs patients. immunologic abnormalities do exist in cf ... | 1992 | 1619343 |
| enteroviral meningitis following haemophilus influenzae type b meningitis. | 1992 | 1628476 | |
| chronic fatigue syndrome. | chronic fatigue syndrome appears to represent a spectrum of disorders in which a variety of pathophysiological mechanisms may operate. while the initiating event in the majority of patients is a pyrexial illness, possibly due to enterovirus infection, evidence of persisting infection or inflammatory changes in muscle and/or brain remain unconvincing. cfs patients display a definite reduced aerobic work capacity compared to normal control subjects, but this may reflect a state of deconditioning r ... | 1992 | 1628478 |
| susceptibility to theiler's virus-induced demyelinating disease correlates with astrocyte class ii induction and antigen presentation. | theiler's murine encephalomyelitis virus (tmev) is a picornavirus which induces a chronic demyelinating disease of the central nervous system (cns) in certain susceptible mouse strains. demyelination has been shown to result from immunopathological responses mediated by cd4+, major histocompatibility complex (mhc) class ii-restricted t cells. as little or no class ii is expressed in the normal mouse cns, the ability of astrocytes to express these proteins and present antigen to t cells from tmev ... | 1992 | 1628891 |
| the role of cd8+t cells in the acute and chronic phases of theiler's murine encephalomyelitis virus-induced disease in mice. | the technique of in vivo depletion with t cell subset-specific monoclonal antibodies was used to study the involvement of cd8+t cells in protection/pathogenesis during the acute and chronic demyelinating phases of theiler's murine encephalomyelitis virus (tmev)-induced disease. mice rendered cd8-deficient prior to infection with tmev were less efficient at clearing virus from the central nervous system compared to intact animals and also suffered demyelinating disease of earlier onset and increa ... | 1992 | 1629706 |
| viral aseptic meningitis in the united states: clinical features, viral etiologies, and differential diagnosis. | 1992 | 1642795 | |
| mapping of a neutralizing antigenic site of coxsackievirus b4 by construction of an antigen chimera. | a neutralizing antigenic site of coxsackievirus b4 (cvb4) was identified by construction of an antigen chimera between coxsackievirus b3 (cvb3) and cvb4. this chimera, designated cvb3/4, was constructed by inserting five amino acids of the putative bc loop of the structural protein vp1 of cvb4 into the corresponding loop of cvb3 by site-directed mutagenesis of infectious recombinant cvb3 cdna. the chimeric cdna was capable of inducing an infectious cycle upon transfection of permissive host cell ... | 1991 | 1645779 |
| role of the humoral immune response in resistance to theiler's virus infection. | theiler's virus, a murine picornavirus, persists in the central nervous system of susceptible strains of mice, causing chronic inflammation and demyelination in the white matter of the spinal cord. resistant strains, however, clear the virus and do not develop late disease. in this study, we compared the characteristics of t and b lymphocytes in c57bl/6 (resistant) and sjl/j (susceptible) mice 1 week after intracerebral infection. we detected a marked increase of the number of immunoglobulin m ( ... | 1991 | 1645797 |
| coxsackie b2 meningitis with unusually high white blood cell count in cerebrospinal fluid. | 1991 | 1645856 | |
| the effect of cyclosporine on the immunopathogenesis of viral myocarditis in mice. | the effect of cyclosporine on the immunopathogenesis of viral myocarditis was studied using a murine viral myocarditis model. mice in the treated group had a higher mortality rate compared with those of the infected control group before day 15 (47/67 vs. 14/31, p less than 0.05). on day 7, treated mice showed higher titers of anti-heart autoantibody than the control group (12 +/- 7 vs 4 +/- 2, p less than 0.05), but no significant difference was seen on day 14 (28 +/- 15 vs. 39 +/- 34). histolog ... | 1991 | 1646340 |
| viral encephalitis. | nonherpetic encephalitis outside the newborn period is usually a self-limited disease. the majority of patients will recover without significant sequelae, and require only supportive therapy during the acute illness. though the underlying viral etiology frequently will escape detection, identification of the infecting agent has considerable prognostic value which can complement clinical measures of severity of disease. the most important initial task of the clinician faced with a case of presump ... | 1991 | 1647166 |
| [immunomodulating properties of non-steroidal anti-inflammatory drugs]. | there has been studied the influence of a number of nonsteroidal antiinflammatory agents (nsaid ) on the humoral immune response of mice in immunization with erythrocytic and viral antigen. it has been found out that nsaid have immunomodulating effect, stimulating humoral immune response (4-iodantipyrine, 4-bromantipyrine) or suppressing it (butadione, sodium salicylate). apparently the mechanism of nsaid ++ immunostimulating effect is related to the inhibition of t-suppressors ++ function by th ... | 1991 | 1647233 |
| presence of enteric viruses in freshwater and their removal by the conventional drinking water treatment process. | a review of results published in english or french between 1980 and 1990 was carried out to determine the levels of indigenous human enteric viruses in untreated surface and subsurface freshwaters, as well as in drinking water that had undergone the complete conventional treatment process. for this purpose, the conventional treatment process was defined as an operation that included coagulation followed by sedimentation, filtration, and disinfection. also assessed was the stepwise efficiency of ... | 1991 | 1647273 |
| [circulation of polioviruses in abiotic objects (review)]. | 1991 | 1647365 | |
| [epidemiologic factors and serum antibody titer to coxsackie b-4 virus in patients with type i diabetes]. | in 150 patients (aged 4-16) with type i diabetes, hospitalized in the paediatric institute in lublin in the years 1968 bis 1979, some epidemiological data were analysed, including age, sex and the month of onset of diabetes. an increase of the number of onsets of diabetes type i was observed in the second half of the year, particularly in september and october, similarly to the occurrence of some virological infections, including coxsackie b-4. our question was if the coxsackie b-4 virus could h ... | 1991 | 1647469 |
| the nucleotide sequence of 3c proteinase region of the coxsackievirus a24 variant: comparison of the isolates in taiwan in 1985-1988. | acute hemorrhagic conjunctivitis caused by coxsackievirus a24 variant (ca24v) first appeared in taiwan in october 1985, followed by two other sequential epidemics in 1986 and 1988. in order to know the evolutionary relationship of the ca24v strains isolated in taiwan, we first determined the nucleotide sequence of the 3c proteinase (3cpro) region of the prototype strain (eh24/70), isolated in singapore in 1970, by molecular cloning. the nucleotide sequence of the 3cpro region thus sequenced show ... | 1991 | 1647565 |
| fate of human enteric viruses, coliphages, and clostridium perfringens during drinking-water treatment. | the elimination of human enteric viruses, coliphages, and clostridium perfringens was studied during a conventional complete drinking-water treatment process. the respective concentrations (geometric mean) of these microorganisms in 100-l samples of river water were, respectively, as follows: viruses, 79 mpniu (most probable number of infectious units) per 100 l, coliphages, 6565 pfu (plaque-forming units) per 100 l. and clostridia, 11,349 cfu (colony-forming units) per 100 l. after predisinfect ... | 1991 | 1647854 |
| lps promotes cb3-induced myocarditis in resistant b10.a mice. | coxsackie virus b3 (cb3) infection of a/j or a.sw mice results in autoimmune myocarditis characterized by a diffuse mononuclear cell infiltrate and heart-specific autoantibodies. c57bl/10 congenic mice that are identically treated are resistant to this disease. cb3-infected resistant b10.a mice were treated with lps to determine if this immunomodulator alters disease susceptibility. in contrast to mice infected only with cb3 or treated only with lps. cb3-infected/lps-treated (cb3/lps) b10.a mice ... | 1991 | 1647882 |
| [the distribution of avian enteroviruses in germany]. | sera from chicken flocks of different regions in germany were examined for antibodies to avian nephritis virus (anv) by indirect immunofluorescence. there was no evidence of a wide distribution of anv, at least in chicken parent flocks, in contrast to results from investigations in england, northern-ireland and japan. | 1991 | 1648474 |
| limb paralysis as a manifestation of coxsackie b virus infection. | forty-four african children (25 girls, 19 boys) are described with single or multiple limb paralysis as a manifestation of infection with coxsackie b virus. single limb paralysis involved exclusively the lower extremity, with no nervous system involvement elsewhere and a relatively benign prognosis. multiple limb paralysis occurred either alone or with other neurological deficits. only two of 15 patients followed up for five months or longer regained premorbid motor status. seven patients requir ... | 1991 | 1648520 |
| nucleic acid detection systems for enteroviruses. | the enteroviruses comprise nearly 70 human pathogens responsible for a wide array of diseases including poliomyelitis, meningitis, myocarditis, and neonatal sepsis. current diagnostic tests for the enteroviruses are limited in their use by the slow growth, or failure to grow, of certain serotypes in culture, the antigenic diversity among the serotypes, and the low titer of virus in certain clinical specimens. within the past 6 years, applications of molecular cloning techniques, in vitro transcr ... | 1991 | 1649002 |
| [virological surveillance of acute respiratory tract illnesses of children in morioka, japan. i. epidemiological patterns of infection with respiratory viruses over a 10-year period]. | a virological surveillance of acute respiratory tract illnesses (rti) of children in morioka, japan, was maintained from september 1973 to december 1983. nasal and throat swabs were collected from 4,334 children with rti. these patients consisted of 3,500 children (80.8%) with upper rti (urti) and 834 children (16.2%) with lower rti. when these patients with urti were classified by maximum temperature recorded into 4 groups (less than or equal to 37.0 degrees c, 37.1-37.9 degrees c, 38.0-38.4 de ... | 1991 | 1649233 |
| effect of relative humidity and air temperature on survival of hepatitis a virus on environmental surfaces. | stainless steel disks (diameter, 1 cm) were contaminated with fecally suspended hepatitis a virus (hav; strain hm-175) and held at low (25% +/- 5%), medium (55% +/- 5%), high (80% +/- 5%), or ultrahigh (95% +/- 5%) relative humidity (rh) at an air temperature of 5,20, or 35 degrees c. hav survival was inversely proportional to the level of rh and temperature, and the half-lives of the virus ranged from greater than 7 days at the low rh and 5 degrees c to about 2 h at the ultrahigh rh and 35 degr ... | 1991 | 1649579 |
| acute respiratory viral infections in ambulatory children of urban northeast brazil. | the morbidity of acute respiratory infections in young children and the role of respiratory viruses were evaluated in a 29-month household-based study in an impoverished urban population in fortaleza, brazil; subjects were 175 children less than 5 years of age in 63 families. home visits were conducted three times weekly during which staff recorded the presence of respiratory and systemic symptoms and collected upper respiratory tract samples for viral isolation. a large and sustained burden of ... | 1991 | 1649871 |
| expression of perforin in infiltrating cells in murine hearts with acute myocarditis caused by coxsackievirus b3. | cell-mediated autoimmunity has been strongly implicated in the pathogenesis of viral myocarditis. | 1991 | 1650300 |
| poliomyelitis-like paralysis during recovery from acute bronchial asthma: possible etiology and risk factors. | a poliomyelitis-like paralytic disease during recovery from an attack of bronchial asthma is described in two young children. they presented at the age of 13 and 22 months, respectively, with acute flaccid paralysis of one or both lower limbs and preserved sensation. cerebrospinal fluid examinations revealed mild protein elevation in both and pleocytosis in the second infant. enteroviruses were isolated in a nasal swab and stools of the second patient. acute onset of flaccid paralysis with absen ... | 1991 | 1650449 |
| animal serum factor(s) causing adverse effects on rias of human enterovirus igm. | the effects of animal sera used at various concentrations in dilution buffers for radioimmunoassays (rias) of human enterovirus-igm were studied. the origin of the sera had no impact on the titres, but adverse effects on virus-specificity tests were noted when sera from some species were used. in attempts to block the binding of 35s-labelled virus by adding unlabelled virus, sera from cow, horse and lamb and from swine and man could usually not be used; instead of a blocking effect, an increase ... | 1991 | 1650788 |
| susceptibility of normal human joint tissue to viruses. | a model system has been developed to investigate the comparative ability of different viruses to replicate and persist intraarticularly. the viruses chosen for study were rubella, mumps, coxsackie b4, adenovirus and varicella zoster, a selection of viruses with different degrees of association with joint symptoms in clinical studies. our results showed that these viruses demonstrated a range of abilities to infect and persist in human joint tissue cultured in vitro. the most arthritogenic viruse ... | 1991 | 1650840 |
| studies on the epidemiology of child infections in the bari area. i. current state of immunity against coxsackie b viruses. | the presence and titer of neutralizing antibodies against coxsackie b viruses were investigated in 1,944 children under 15 years of age living in bari and its area. in 1,781 out of 1,944 sera (91.6%), neutralizing antibodies were present against at least one of the strains tested: 333 (17.1%) samples were found to be positive for one serotype only, 412 (21.2%) for 2 serotypes, 394 (20.3%) for 3, 394 (18.0%) for 4, 199 (10.2%) for 5 and 94 (4.8%) for all 6 strains tested. of the 1,944 serums exam ... | 1991 | 1650961 |
| [coxsackie b4 infection, myopericarditis and diabetes mellitus]. | the authors described a case of acute miopericarditis with evolution towards dilated cardiomiopathy, secondary to coxsackie b4 infection, in a patient developing a concomitant insulin dependent diabetes (didm), admitted to be also an expression of this infection. the pathogenesis and the immunological basis of this aggression is discussed and some therapeutic considerations made. | 1991 | 1651046 |
| nucleotide sequences important for translation initiation of enterovirus rna. | an infectious cdna clone was constructed from the genome of coxsackievirus b1 strain. a number of rna transcripts that have mutations in the 5' noncoding region were synthesized in vitro from the modified cdna clones and examined for their abilities to act as mrnas in a cell-free translation system prepared from hela s3 cells. rnas that lack nucleotide sequences at positions 568 to 726 and 565 to 726 were found to be less efficient and inactive mrnas, respectively. to understand the biological s ... | 1991 | 1651409 |
| therapeutic effects of bovine enterovirus infection on rabbits with experimentally induced adult t cell leukaemia. | a bovine enterovirus, mz-468, showed cytopathic effects on cell line f-647a, which was established by coculture of human t cell lymphotropic virus type 1-transformed mt-2 cells and x-irradiated rabbit lymphocytes. microcalorimetric assay showed that residual, viable, mz-468-infected f-647a cells produced less heat than non-infected cells. the therapeutic effects of mz-468 infection were examined in rabbits in which adult t cell-like leukaemia (atl) had been induced by inoculation of f-647a cells ... | 1991 | 1651991 |
| herpangina surveillance in japan, 1982-1989. a report of the national epidemiological surveillance of infectious agents in japan. | in japan, herpangina cases increase every summer with an incidence peak in july. during the past eight years from january, 1982 to december, 1989, a total of 3,974 viruses from herpangina cases were reported from 47 participating laboratories, and coxsackie a (ca) viruses accounted for most of them, 3,055 (76.9%). the major types associated with herpangina were, in order of frequency, ca4, 10, 5, 6, 2 and 3, representing 87.2%-80.8% of total isolations reported for each respective type in this p ... | 1991 | 1652037 |
| acute infection of encephalomyocarditis (emc) virus in syrian hamsters. | one-month-old syrian hamsters of the apa and std: golden strains were inoculated intraperitoneally with 10(5) pfu/head of the d variant of encephalomyocarditis (emc) virus and examined virologically and pathologically up to 7 days after inoculation. apa hamsters developed apparent hyperglycemia due to pancreatic islet cell damage while std:golden hamsters did not. hamsters of both strains showed clear histopathologic changes in the testis with prominent viral replication as well as in the brain, ... | 1991 | 1653039 |
| [coxsackie b virus in children with acute myocarditis]. | to study etiopathogenic and follow-up aspects of patients with myocarditis. | 1991 | 1653563 |
| [an experimental study of the effect of radix astragali on mice with viral myocarditis infected by coxsackie b-3]. | 1991 | 1655376 |