Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| nucleocapsid proteins from other swine enteric coronaviruses differentially modulate pedv replication. | porcine epidemic diarrhea virus (pedv), transmissible gastroenteritis virus (tgev) and porcine deltacoronavirus (pdcov) share tropism for swine intestinal epithelial cells. whether mixing of viral components during co-infection alters pathogenic outcomes or viral replication is not known. in this study, we investigated how different coronavirus nucleocapsid (cov n) proteins interact and affect pedv replication. we found that pdcov n and tgev n can competitively interact with pedv n. however, the ... | 2020 | 31756532 |
| mortalin restricts porcine epidemic diarrhea virus entry by downregulating clathrin-mediated endocytosis. | clathrin-mediated endocytosis is a mechanism used for the invasion of cells by a variety of viruses. mortalin protein is involved in a variety of cellular functions and plays a role in viral infection. in this study, we found that mortalin significantly inhibited the replication of porcine epidemic diarrhea virus (pedv) through restricting virus entry. mechanistically, a biochemical interaction between the carboxyl terminus of mortalin and clathrin heavy chain (cltc) was been found, and mortalin ... | 2019 | 31767073 |
| anti-tgev miller strain infection effect of lactobacillus plantarum supernatant based on the jak-stat1 signaling pathway. | transmissible gastroenteritis (tge), caused by transmissible gastroenteritis virus (tgev), is one many gastrointestinal inflections in piglets, characterized by diarrhea, and high mortality. probiotics are ubiquitous bacteria in animal intestines, which have many functions, such as promoting intestinal peristalsis and maintaining the intestinal balance. we found that the supernatant of the lp-1 strain of lactobacillus plantarum, isolated in our laboratory, and named lp-1s had marked anti-tgev ef ... | 2019 | 31781061 |
| surface-displayed porcine ifn-λ3 in lactobacillus plantarum inhibits porcine enteric coronavirus infection of porcine intestinal epithelial cells. | interferon (ifn)-λ plays an essential role in mucosal cells which exhibit strong antiviral activity. lactobacillus plantarum (l. plantarum) has substantial application potential in the food and medical industries because of its probiotic properties. alphacoronaviruses, especially porcine epidemic diarrhea virus (pedv) and transmissible gastroenteritis virus (tgev), cause high morbidity and mortality in piglets resulting in economic loss. co-infection by these two viruses is becoming increasingly ... | 2020 | 31838830 |
| minimum determinants of transmissible gastroenteritis virus enteric tropism are located in the n-terminus of spike protein. | transmissible gastroenteritis virus (tgev) is an enteric coronavirus causing high morbidity and mortality in porcine herds worldwide, that possesses both enteric and respiratory tropism. the ability to replicate in the enteric tract directly correlates with virulence, as tgevs with an exclusive respiratory tropism are attenuated. the tissue tropism is determined by spike (s) protein, although the molecular bases for enteric tropism remain to be fully characterized. both papn and sialic acid bind ... | 2019 | 31861369 |
| prevalence and phylogenetic analysis of porcine diarrhea associated viruses in southern china from 2012 to 2018. | in china, large-scale outbreaks of severe diarrhea caused by viruses have occurred in pigs since late 2010. to investigate the prevalence and genetic evolution of diarrhea-associated viruses responsible for the outbreaks, a total of 2987 field diarrheal samples collected from 168 pig farms in five provinces in southern china during 2012-2018 were tested. | 2019 | 31881873 |
| transmissible gastroenteritis virus targets paneth cells to inhibit the self-renewal and differentiation of lgr5 intestinal stem cells via notch signaling. | infection with transmissible gastroenteritis virus (tgev) has been associated with villous atrophy within 48 h, which seriously disrupts intestinal homeostasis. however, the underlying mechanisms remain elusive. in this study, we found that tgev infection severely disrupted intestinal homeostasis via inhibition of self-renewal and differentiation in lgr5 intestinal stem cells (iscs). profoundly, tgev-encoded nsp10/nsp16 protein complex-mediated the inactivation of notch signaling provided a mech ... | 2020 | 31959773 |
| dynamics of transmissible gastroenteritis virus internalization unraveled by single-virus tracking in live cells. | transmissible gastroenteritis virus (tgev) is a swine enteropathogenic coronavirus that causes significant economic losses in swine industry. current studies on tgev internalization mainly focus on viral receptors, but the internalization mechanism is still unclear. in this study, we used single-virus tracking to obtain the detailed insights into the dynamic events of the tgev internalization and depict the whole sequential process. we observed that tgevs could be internalized through clathrin- ... | 2020 | 32017270 |
| transmissible gastroenteritis virus infection up-regulates fcrn expression via nucleocapsid protein and secretion of tgf-β in porcine intestinal epithelial cells. | transmissible gastroenteritis virus (tgev) is a porcine intestinal coronavirus that causes fatal severe watery diarrhea in piglets. the neonatal fc receptor (fcrn) is the only igg transport receptor, its expression on mucosal surfaces is triggered upon viral stimulation, which significantly enhances mucosal immunity. we utilized tgev as a model pathogen to explore the role of fcrn in resisting viral invasion in overall intestinal mucosal immunity. tgev induced fcrn expression by activating nf-κb ... | 2019 | 32038538 |
| the use of cells from anpep knockout pigs to evaluate the role of aminopeptidase n (apn) as a receptor for porcine deltacoronavirus (pdcov). | the coronaviruses, porcine epidemic diarrhea virus (pedv), transmissible gastroenteritis virus (tgev), and porcine deltacoronavirus (pdcov) represent important sources of neonatal diarrhea on pig farms. the requirement for aminopeptidase n (apn) as a receptor for tgev, but not for pedv, is well established. in this study, the biological relevance of apn as a receptor for pdcov was tested by using crispr/cas9 to knockout the apn gene, anpep, in pigs. porcine alveolar macrophages (pams) from anpep ... | 2020 | 32056711 |
| decreased nhe3 activity and trafficking in tgev-infected ipec-j2 cells via the sglt1-mediated p38 mapk/akt2 pathway. | transmissible gastroenteritis virus (tgev) primarily replicates in intestinal epithelial cells and causes severe damage to host cells, resulting in diarrhea. surface nhe3 serves as the key regulatory site controlling electroneutral na+ absorption. in this study, our results showed that the surface nhe3 content was significantly reduced following tgev infection, whereas the total level of protein expression was not significantly changed, and nhe3 activity gradually decreased with prolonged infect ... | 2020 | 32070687 |
| structural basis for inhibiting porcine epidemic diarrhea virus replication with the 3c-like protease inhibitor gc376. | porcine epidemic diarrhea virus (pedv), being highly virulent and contagious in piglets, has caused significant damage to the pork industries of many countries worldwide. there are no commercial drugs targeting coronaviruses (covs), and few studies on anti-pedv inhibitors. the coronavirus 3c-like protease (3clpro) has a conserved structure and catalytic mechanism and plays a key role during viral polyprotein processing, thus serving as an appealing antiviral drug target. here, we report the anti ... | 2020 | 32098094 |
| identification and characterization of linear b cell epitopes on the nucleocapsid protein of porcine epidemic diarrhea virus using monoclonal antibodies. | the nucleocapsid (n) protein of porcine epidemic diarrhea virus (pedv), the most important pathogen causing severe diarrhea in piglets, is a highly conserved structural protein. in this study, 5 monoclonal antibodies (mcabs) against the pedv n-protein were prepared and identified. three new epitopes, 56qirwrmrrgeri67, 318gyaqiaslapnvaallfggnva vre342 and 398heeaiyddv406, were firstly identified in the viral n-protein, by using mcabs 3f10, 6a11, and 1c9. the epitope 398heeaiyddv406 was deleted in ... | 2020 | 32142743 |
| establishment of method for dual simultaneous detection of pedv and tgev by combination of magnetic micro-particles and nanoparticles. | transmissible gastroenteritis virus (tgev) and porcine epidemic diarrhea virus (pedv) are the main pathogens causing viral diarrhea in pig, mixed infections of these two viruses are very common in intensive pig rearing. however, there is a lack of a method to simultaneously detect and distinguish pedv and tgev in preclinical levels. in this study, we aimed to establish a dual ultrasensitive nanoparticle dna probe-based pcr assay (dual undp-pcr) based on functionalized magnetic bead enrichment an ... | 2020 | 32147375 |
| characterization and evaluation of the pathogenicity of a natural recombinant transmissible gastroenteritis virus in china. | porcine transmissible gastroenteritis virus (tgev) is one of the major etiological agents of viral enteritis and fetal diarrhea in suckling piglets. in this study, a tgev js2012 strain was isolated from the feces of piglets in jiangsu province, china. the phylogenetic analysis showed that tgev js2012 was placed between the purdue and the miller clusters. analysis of recombination confirmed that tgev js2012 is a natural recombinant strain between miller m6 and purdue 115. similar to miller m6, vi ... | 2020 | 32174456 |
| cultivation techniques for animal coronaviruses: emphasis on feline infectious peritonitis virus, canine coronavirus, transmissible gastroenteritis virus, and porcine hemagglutinating encephalomyelitis virus. | techniques are described for the growth and characterization of some mammalian coronaviruses. because of the fastidious nature of their growth requirements, most will replicate only in cells derived from the natural host or a closely related species. fetal cat cells are used to grow fipv, and porcine cells are used to grow tgev and hev. however, ccv will replicate in both feline and canine cells. although all four of these viruses prefer to replicate in a cell in the stationary phase of growth, ... | 1988 | 32214595 |
| effects of humidity and other factors on the generation and sampling of a coronavirus aerosol. | suspensions of transmissible gastroenteritis virus (tgev), a porcine coronavirus, were nebulized at rates of 0.1-0.2 ml/min into moving air using a collison nebulizer or a plastic medical nebulizer operating at pressures ranging from 7 to 15 psi. the airborne viruses were collected on heating, ventilating, and air conditioning (hvac) filters in an experimental apparatus and also sampled upstream of these test filters using agi-30 and biosampler impinger samplers. to study the effects of relative ... | 2007 | 32214623 |
| retrospective serological survey on selected viral pathogens in wild boar populations in germany. | the objective of this study was to retrospectively evaluate the occurrence of porcine parvovirus (ppv), aujeszky's disease virus (adv), transmissible gastroenteritis virus (tgev), porcine respiratory coronavirus (prcv), porcine reproductive and respiratory syndrome virus (prrsv) and swine influenza virus (siv) in selected wild boar populations in germany (n = 1,221). commercial enzyme linked immunosorbent assay and hemagglutination inhibition tests were used for serological monitoring. the sero ... | 2009 | 32214937 |
| a serological survey of selected pathogens in wild boar (sus scrofa) in northern turkey. | during the hunting season in march 2012, a total of 93 blood samples were collected from wild boars (sus scrofa) shot in the area of northern turkey (samsun and gumushane provinces). these blood samples were examined by enzyme immunoassay (elisa) for the presence of antibodies to classical swine fever virus (csfv), aujeszky's disease virus (adv), porcine reproductive and respiratory syndrome virus (prrsv), porcine respiratory coronavirus (prcv), swine influenza virus (siv), porcine parvovirus (p ... | 2013 | 32214940 |
| cloning and expression of nucleocapsid protein gene of tgev hb06 strain. | the nucleocapsid protein gene of transmissible gastroenteritis virus, 1 149 bp in length, was amplified by rt-pcr from isolated strain hb06 and cloned into pmd18-t. sequence comparison with other transmissible gastroenteritis virus (tgev) strains selected from the gene bank revealed that the homology of n gene complete sequence shares more than 97% in nucleotide. n gene was cloned into bamhi and ecori multiple cloning sites of the prokaryotic expression vector pet 20 b, and named petn. after bei ... | 2007 | 32214985 |
| prophylactic and therapeutic effects of egg yolk immunoglobulin against porcine transmissible gastroenteritis virus in piglets. | porcine transmissible gastroenteritis virus (tgev) is the causative agent of acute diarrhea of newborn piglets that provokes high mortality rates in affected farms. in this study, specific immunoglobulin from egg yolk against tgev was produced by immunization of white leghorn hens. enzyme-linked immunosorbent assay (elisa) and virus neutralization (vn) test revealed that the specific antibody titer started to increase on the tenth day post-immunization, reached its peak on the eighth week, and r ... | 2009 | 32214986 |
| preparation and physicochemical property of chicken yolk immunoglobulin (igy) against porcine transmissible gastroenteritis virus (tgev). | oral administration of immunoglobulin prepared from the egg yolk of hens immunized with porcine transmissible gastroenteritis virus (tgev) has been demonstrated to reduce piglets mortality significantly in our previous studies. in the present study, we investigated the stability of chicken yolk immunoglobulin (igy) specific to tgev by measuring the remaining activity by elisa. the results showed that the igy was stable between ph 4 and ph 11. in the incubation with pepsin at ph 4 and ph 6, about ... | 2009 | 32214987 |
| cloning and expression of the membrane protein gene of tgev hb06 strain. | the membrane protein gene(m) of transmissible gastroenteritis virus (tgev) strain hb06, isolated from the feces of piglets infected with tgevon a pig farm in hebei province, was amplified by reverse transcriptase-polymerase chain reaction (rt-pcr). the amplified pcr products of tgev hb06 were cloned, sequenced, and compared with other tgev strains genes selected from the genbank. then, the recombinant fragment in pmd18-t was subcloned into corresponding sites of prokaryotic expression vector pge ... | 2010 | 32214988 |
| role of porcine aminopeptidase n and sialic acids in porcine coronavirus infections in primary porcine enterocytes. | porcine epidemic diarrhea virus (pedv) and transmissible gastroenteritis virus (tgev) have been reported to use aminopeptidase n (apn) as a cellular receptor. recently, the role of apn as a receptor for pedv has been questioned. in our study, the role of apn in pedv and tgev infections was studied in primary porcine enterocytes. after seven days of cultivation, 89% of enterocytes presented microvilli and showed a two- to five-fold higher susceptibility to pedv and tgev. a significant increase of ... | 2020 | 32260595 |
| biological and antigenic relationships between virus-induced porcine and human interferons. | endogenous interferon (ifn) made by the newborn piglet in response to enteric coronavirus tgev (transmissible gastroenteritis virus) infection was identified as leukocytic ifn (ifn-α). indeed, the antiviral activity found in the serum and in the urine of infected piglets displayed the same main biological and antigenic properties as the ifn induced in influenza-infected pig leukocytes. it is therefore concluded that most, if not all, circulating ifn activity must be derived from lymphoid cells. ... | 2020 | 32288182 |
| inhibition of transmissible gastroenteritis coronavirus (tgev) multiplication in vitro by non-immune lymphocytes. | in vitro studies were undertaken to examine the effects of non-immune porcine peripheral blood leukocytes (pbl) on a coronavirus infection due to transmissible gastroenteritis virus (tgev). the assay consisted of tgev-infected epithelial cells expressing viral antigens on the cell surface and producing low amounts of interferon (ifn). non-immune pbl were found to limit virus replication at an effector-to-target ratio of 100/1 even when effector cells were depleted of phagocytic cells. neutralizi ... | 2020 | 32288183 |
| studies on mucosal immunity induced by transmissible gastroenteritis virus nucleocapsid protein recombinant lactobacillus casei in mice and sow. | mucosal immunity plays an important role in protecting pigs against transmissible gastroenteritis virus (tgev) infection. to elicit mucosal immune response against tgev, we developed a surface antigen display system using the poly-[.gamma]-glutamate synthetase a (pgsa) protein of bacillus subtilis as an anchoring matrix to express recombinant fusion proteins of pgsa and nucleocapsid protein of tgev in lactobacillus casei. surface location of fusion protein was verified by elisa and indirect immu ... | 2009 | 32288751 |
| status report on porcine epidemic diarrhea virus in the united states. | on 16 may 2013, the usda animal and plant health inspection service, national veterinary services laboratories reported the detection of porcine epidemic diarrhea virus in the united states for the first time.this virus causes severe diarrhea and vomiting in young pigs. porcine epidemic diarrhea virus does not infect humans and is not a food safety risk.this virus is already found in many countries around the world, and there is no us official regulation of the virus and no export restrictions t ... | 2014 | 32288969 |
| prevalence of antibodies against transmissible gastroenteritis virus (tgev) in hungary. | transmissible gastroenteritis (tge) is a highly contagious enteric disease of swine, which became infrequent with the appearance of porcine respiratory coronavirus (prcv). tge was last reported in hungary in 2013 and the virus has not been found since, therefore a serological survey was planned to estimate the level of protection against it. 908 sera of sows from 93 farms were selected together with 174 archive samples from one farm covering a wider age group. all samples were screened with an i ... | 2019 | 32289094 |
| detecting and monitoring porcine hemagglutinating encephalomyelitis virus, an underresearched betacoronavirus. | members of family coronaviridae cause a variety of diseases in birds and mammals. porcine hemagglutinating encephalomyelitis virus (phev), a lesser-researched coronavirus, can infect naive pigs of any age, but clinical disease is observed in pigs ≤4 weeks of age. no commercial phev vaccines are available, and neonatal protection from phev-associated disease is presumably dependent on lactogenic immunity. although subclinical phev infections are thought to be common, phev ecology in commercial sw ... | 2020 | 32376700 |
| application of nanomaterials in treatment, anti-infection and detection of coronaviruses. | nanotechnology and nanomedicine have excellent potential in dealing with a range of different health problems, including viruses, which are considered to be a serious challenge in the medical field. application of nanobiotechnology could represent a new avenue for the treatment or disinfection of viruses. there is increasing concern regarding the control of coronaviruses, among these, middle east respiratory syndrome coronavirus, severe acute respiratory syndrome coronavirus and severe acute res ... | 2020 | 32378459 |
| rapid and visual detection of porcine deltacoronavirus by recombinase polymerase amplification combined with a lateral flow dipstick. | porcine deltacoronavirus (pdcov) is a newly emerging coronavirus that was first identified in 2012 in hong kong, china. since then, pdcov has subsequently been reported worldwide, causing a high number of neonatal piglet deaths and significant economic losses to the swine industry. therefore, it is necessary to establish a highly sensitive and specific method for the rapid diagnosis of pdcov. | 2020 | 32381014 |
| prevalence and genomic characteristics of a novel reassortment mammalian orthoreovirus type 2 in diarrhea piglets in sichuan, china. | mammalian orthoreovirus (mrv), which can infect almost all mammals, is a zoonotic virus. in this study, six strains of type 2 mrv (mrv2) were isolated from 7 diarrhea piglets from a farm that had an outbreak of diarrhea in piglets in 2018, which tested negative for porcine epidemic diarrhea virus (pedv), porcine transmissible gastroenteritis virus (tgev), porcine deltacoronavirus (pdcov), and porcine group a rotavirus (rav). the mrv2 isolate infected neonatal piglets, in which it induced severe ... | 2020 | 32544614 |
| agrodiag porcov: a multiplex immunoassay for the differential diagnosis of porcine enteric coronaviruses. | three different porcine enteric coronaviruses (pecs), i.e., porcine epidemic diarrhea virus (pedv), transmissible gastroenteritis virus (tgev) and porcine deltacoronavirus (pdcov) are currently circulating in u.s. commercial swine herds. differential diagnosis of pecs relies on laboratory methods. this study describes the development of an elisa-like multiplex planar immunoassay based on virus-specific recombinant s1 proteins printed in an array of spots at the bottom of a 96-well microplate for ... | 2020 | 32562689 |
| novel sars-cov-2/covid-19: origin, pathogenesis, genes and genetic variations, immune responses and phylogenetic analysis. | in this review, we focused on the origins of the novel coronavirus (sars-cov-2), origin, pathogenesis, immune responses, genes and genetic variations, phylogenetic analyses, and potential therapeutic strategies to summarize approaches for developing broadly effective preventions and vaccines to cope covid-19. towards the end of 2019, sars-cov-2 has emerged in association with the sars, later was named covid-19 caused an environment of chaos worldwide and infected a massive number of lives. since ... | 2020 | 32566803 |
| microrna-4331-5p promotes fmdv replication through inhibiting interferon pathways in pk-15 cells. | micrornas play vital roles in regulating the battle between pathogens and host cells during viral challenging. mir-4331 aggravates transmissible gastroenteritis virus (tgev) -induced mitochondrial damage, also suppresses transcription of tgev gene 7 via targeting cellular cdca7. otherwise, mir-4331-5p affects h1n1/2009 influenza a virus replication by targeting viral ha and ns. however, whether microrna ssc-mir-4331-5p (mir-4331-5p) regulates foot and mouth virus (fmdv) replication remains uncle ... | 2020 | 32574680 |
| molecular diversity of alphacoronavirus 1 in dogs and cats in colombia. | alphacoronavirus 1 (subgenus tegacovirus, genus alphacoronavirus, family coronaviridae), which encompasses transmissible gastroenteritis virus (tgev), feline coronavirus (fcov) and canine coronavirus (ccov), is an important pathogen that can cause severe gastroenteritis and is distributed worldwide. ccov has two different genotypes: ccov type i, which has a high identity with fcov-i, and ccov type ii, which is divided into two subtypes, ccov iia (pantropic) and ccov iib, which is related to fcov ... | 2020 | 32665984 |
| antiviral and virucidal effects of curcumin on transmissible gastroenteritis virus in vitro. | emerging coronaviruses represent serious threats to human and animal health worldwide, and no approved therapeutics are currently available. here, we used transmissible gastroenteritis virus (tgev) as the alpha-coronavirus model, and investigated the antiviral properties of curcumin against tgev. our results demonstrated that curcumin strongly inhibited tgev proliferation and viral protein expression in a dose-dependent manner. we also observed that curcumin exhibited direct virucidal abilities ... | 2020 | 32677610 |
| postweaning mortality in commercial swine production ii: review of infectious contributing factors. | postweaning mortality is extremely complex with a multitude of noninfectious and infectious contributing factors. in the current review, our objective is to describe the current state of knowledge regarding infectious causes of postweaning mortality, focusing on estimates of frequency and magnitude of effect where available. while infectious mortality is often categorized by physiologic body system affected, we believe the complex multifactorial nature is better understood by an alternative stra ... | 2020 | 32705048 |
| genus-specific pattern of intrinsically disordered central regions in the nucleocapsid protein of coronaviruses. | the nucleocapsid (n) protein is conserved in all four genera of the coronaviruses, namely alpha, beta, gamma, and delta, and is essential for genome functionality. bioinformatic analysis of coronaviral n sequences revealed two intrinsically disordered regions (idrs) at the center of the polypeptide. while both idr structures were found in alpha, beta, and gamma-coronaviruses, the second idr was absent in deltacoronaviruses. two novel coronaviruses, currently placed in the gammacoronavirus genus, ... | 2020 | 32765822 |
| next-generation porcine intestinal organoids: an apical-out organoid model for swine enteric virus infection and immune response investigations. | the intestinal organoid culture system is a pathbreaking working model for investigating pathogen-host interactions in the intestines. however, due to the limitations of the first generation of intestinal organoids, basal-out structure and growth in matrigel, most pathogens can rarely attach to the apical membrane directly and hardly initiate infection. in this study, we first developed a next-generation porcine intestinal organoid culture system, characterized by an apical membrane on the surfa ... | 2020 | 32796075 |
| cd163 and papn double-knockout pigs are resistant to prrsv and tgev and exhibit decreased susceptibility to pdcov while maintaining normal production performance. | porcine reproductive and respiratory syndrome virus (prrsv) and transmissible gastroenteritis virus (tgev) are two highly infectious and lethal viruses causing major economic losses to pig production. here, we report generation of double-gene-knockout (dko) pigs harboring edited knockout alleles for known receptor proteins cd163 and papn and show that dko pigs are completely resistant to genotype 2 prrsv and tgev. we found no differences in meat-production or reproductive-performance traits betw ... | 2020 | 32876563 |
| sars-cov-2 in environmental perspective: occurrence, persistence, surveillance, inactivation and challenges. | the unprecedented global spread of the severe acute respiratory syndrome (sars) caused by sars-cov-2 is depicting the distressing pandemic consequence on human health, economy as well as ecosystem services. so far novel coronavirus (cov) outbreaks were associated with sars-cov-2 (2019), middle east respiratory syndrome coronavirus (mers-cov, 2012), and sars-cov-1 (2003) events. cov relates to the enveloped family of betacoronavirus (βcov) with positive-sense single-stranded rna (+ssrna). knowing ... | 2021 | 32901196 |
| rhodanine derivative lj001 inhibits tgev and pdcov replication in vitro. | transmissible gastroenteritis virus (tgev) and porcine deltacoronavirus (pdcov) are members of the family coronaviridae and mainly cause acute diarrhea/vomiting, dehydration and mortality in piglets, which lead to huge economic losses to the swine industry. rhodanine derivative lj001 has been verified to be effective against some enveloped virus infections in vitro. in this study, we evaluated the antiviral activity of lj001 towards tgev and pdcov replication on swine testicular(st) cells. our r ... | 2020 | 32956749 |
| nitazoxanide and jib-04 have broad-spectrum antiviral activity and inhibit sars-cov-2 replication in cell culture and coronavirus pathogenesis in a pig model. | pathogenic coronaviruses represent a major threat to global public health. here, using a recombinant reporter virus-based compound screening approach, we identified several small-molecule inhibitors that potently block the replication of the newly emerged severe acute respiratory syndrome virus 2 (sars-cov-2). two compounds, nitazoxanide and jib-04 inhibited sars-cov-2 replication in vero e6 cells with an ec 50 of 4.90 μm and 0.69 μm, respectively, with specificity indices of greater than 150. b ... | 2020 | 32995798 |
| antiviral effects of bovine antimicrobial peptide against tgev in vivo and in vitro. | in suckling piglets, transmissible gastroenteritis virus (tgev) causes lethal diarrhea accompanied by high infection and mortality rates, leading to considerable economic losses. this study explored methods of preventing or inhibiting their production. bovine antimicrobial peptide-13 (apb-13) has antibacterial, antiviral, and immune functions. | 2020 | 33016025 |
| covid 19 an infectious disease influenced in modern era - recent survey in india. | the virus which is called as corona spoiling many of human lives as well as countries growth. coronavirus is an infectious disease, so spreading from one person to other easily. till now there is no medicine for corona. world health organization (who) taking lot of steps to control it. in india many of them get affected by coronavirus called covid-19. a household survey is conducted nationally through online. this survey is a method for checking knowledge about the infectious virus. in this onli ... | 2020 | 33078095 |
| assessments of different inactivating reagents in formulating transmissible gastroenteritis virus vaccine. | transmissible gastroenteritis virus (tgev) causes enteric infection in piglets, characterized by vomiting, severe diarrhea and dehydration, and the mortality in suckling piglets is often high up to 100%. vaccination is an effective measure to control the disease caused by tgev. | 2020 | 33097081 |
| evolution of transmissible gastroenteritis virus (tgev): a codon usage perspective. | transmissible gastroenteritis virus (tgev) is a coronavirus associated with diarrhea and high mortality in piglets. to gain insight into the evolution and adaptation of tgev, a comprehensive analysis of phylogeny and codon usage bias was performed. the phylogenetic analyses of maximum likelihood and bayesian inference displayed two distinct genotypes: genotypes i and ii, and genotype i was classified into subtypes ia and ib. the compositional properties revealed that the coding sequence containe ... | 2020 | 33114322 |
| production of monoclonal antibodies targeting aminopeptidase n in the porcine intestinal mucosal epithelium. | porcine aminopeptidase n (apn), a membrane-bound metallopeptidase abundantly present in small intestinal mucosa, can initiate a mucosal immune response without any interference such as low protein expression, enzyme inactivity, or structural changes. this makes apn an attractive candidate in the development of vaccines that selectively target the mucosal epithelium. previous studies have shown that apn is a receptor protein for both enterotoxigenic escherichia coli (e. coli) f4 and transmissible ... | 2021 | 34096912 |