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[human papillomavirus--(hpv)/balanoposthitis--a new disease?].two cases of symptom-producing balanoposthitis associated with human papillomavirus infection are described. these patients had spotty, macular, erythematous rash on the glans penis and prepuce. the symptoms and objective findings resemble those of candida infection but the diagnosis can be established by histological examination of biopsy from the acetowhite elements.19911850881
isolation of cellular revertants from a rat cell line transformed by the e6 and e7 genes of human papillomavirus type 16.three revertants defective in the ability to form colonies in semisolid medium were isolated from a rat cell line transformed by the e6 and e7 genes of human papillomavirus type 16 (hpv16). these revertants appeared to be defective in a cellular factor(s) necessary for transformation by hpv16-e6e7 genes since they still expressed a comparable amount of hpv16-e6e7 mrna and e7 protein to the parental cells, harbored rescuable transforming virus, and were resistant to retransformation by hpv16-e6e7 ...19911850909
expression of human papillomavirus proteins in yeast saccharomyces cerevisiae.the l1 and l2 proteins of human papillomavirus (hpv) types 1, 6, and 16 and the e6 and e7 proteins of hpv 16 were expressed in saccharomyces cerevisiae. the yeast expressed proteins were readily detected by immune blotting and were generally intact. the hpv 1 l1 and l2 proteins expressed in yeast were indistinguishable from the major and minor capsid proteins purified from hpv 1 virions as judged by gel electrophoresis and immunoblotting. the hpv 6 and hpv 16 l2 proteins and hpv 16 e7 proteins w ...19911850917
antibodies to human papillomavirus type-16 in human sera as revealed by the use of prokaryotically expressed viral gene products.open reading frames of human papillomaviruses were expressed in escherichia coli as beta-galactosidase fusion proteins. these bacterially derived papillomaviral gene products were used to examine sera from 67 women (63 healthy subjects, 4 patients with genital carcinoma) for antibodies to papillomavirus type-16 antigens (e1, e2, e4, e5, e6, e7, l1, l2) and the l2 proteins of hpv-6b and hpv-18 by western-blot analysis. the serologic data were compared with cytological findings classified accordin ...19911850922
human papillomavirus type 16 transcripts expressed from viral-cellular junctions and full-length viral copies in caski cells and in a cervical carcinoma.we have mapped using the rna pcr the viral-cellular junctions of hpv16 viral-cellular cotranscripts expressed in caski cells and a cervical carcinoma to nt 3728 and 881, respectively. both junctions were located within the e1-e2 region. examination of the cellular sequences of the cotranscripts showed the presence of a polyadenylation signal in each of the transcripts. in caski cells and in the cervical carcinoma transcripts derived from the full-length early region including the e2 transcript w ...19911850935
diagnostic sensitivity of polymerase chain reaction and southern blot hybridization for the detection of human papillomavirus dna in biopsy specimens from cervical lesions.human papillomaviruses (hpvs) are associated with benign and malignant neoplasms of the cervix. one of the criteria for their etiologic role requires an assessment of whether virtually all or only a small fraction of lesions contain viral genomes. dna preparations from colposcopically directed punch biopsies of cervical lesions were analyzed by southern blot hybridization and the polymerase chain reaction (pcr) for the presence of hpv dna. the biopsy specimens represented different pathologic en ...19911850950
human papillomavirus types 16 and 18 in adenocarcinoma of the uterine cervix.many reports have shown a link between human papillomavirus (hpv) and cervical squamous neoplasia. however, the association of hpv with cervical adenocarcinoma has been studied less extensively. the authors evaluated the presence of hpv-dna in 106 patients with adenocarcinoma of the uterine cervix by in situ hybridization, using 35s-labeled probes for hpv 16 dna and hpv 18 dna. the overall prevalence of hpv-dna was 18% (19 of 106). hpv 16 was present in 2 (2%) cases, hpv 18 was observed in 15 (1 ...19911850951
in vitro photodynamic treatment of normal and human papilloma virus-transfected keratinocytes with photofrin ii and red light.photodynamic therapy involves the use of light of appropriate wavelength to excite a photosensitizer to result in tissue destruction. the photosensitizer dihematoporphyrin ether and red light were used to treat normal and human papilloma virus type 18-transfected keratinocytes in vitro. although both cell lines were sensitive to treatment, normal keratinocytes retained more dihematoporphyrin ether and were more sensitive to photodynamic therapy than were transfected cells. in vitro data fail to ...19911850973
dna-cytometric detection of euploid polyploidization in oral lichen ruber planus.the dna distribution was analyzed in 29 cases of oral lichen ruber planus that were negative for human papillomavirus and not suspected of being precancerous. monolayer smears prepared from formalin-fixed, paraffin-embedded tissues were automatically feulgen stained and used for rapid interactive dna cytometry via a tv-based image analysis system combined with an automated microscope. nuclei with dna contents greater than 4c were found in 25 cases (86%). dna contents greater than 8c were seen in ...19911851011
human papillomavirus dna as a possible index of invasiveness in female genital tract carcinomas.paraffin-embedded tumour sections were used for the polymerase chain reaction (pcr) with three primer sets that amplify specific regions of human papillomavirus (hpv) types 11, 16 and 18. the positive samples were confirmed by hybridisation of the amplified sequences with the specific hpv probes. in all screened metastases the same viral sequences were found as in the primary tumour. hpv 16 was the most frequently detected virus in genital tract tumours. in a metastatic ovary carcinoma with unkn ...19911851022
human papillomavirus dna in cervical intraepithelial neoplasia detected by in situ hybridisation.human papillomavirus (hpv) infection was investigated by in situ hybridisation in histological sections from 38 women with abnormal papanicolaou smears. 13 patients had condylomatous lesions without atypia, 15 cervical intraepithelial neoplasia (cin) i, 4 cin ii, 3 cin iii and 2 carcinoma in situ (cis). hpv dna was detected in 29 cases (78%) (1 specimen was technically inadequate). hpv 16 and 18, and 31, 33 and 35 were both present (67%) in cin iii. hpv 6 and 11 were more frequent in cin i (56%) ...19911851023
human papilloma virus and carcinoma of the laryngopharynx.the presence of human papillomavirus (hpv) genomes 6, 11, 16 and 33 in 16 patients with laryngopharyngeal carcinomas was examined by means of a polymerase chain reaction. hpv genomes were detected in 75 per cent of cases. in hypopharyngeal carcinoma, hpv 6 and 11 were present in adjacent tissue but absent from the tumour. hpv 16 and 33 were present in 40 per cent of laryngeal tumours and adjacent tissue. these results suggest that hpv 16 and 33 may be closely associated with the development of s ...19911851198
human papillomavirus dna sequences in cell lines derived from head and neck squamous cell carcinomas.there is increasing evidence that human papillomaviruses (hpv) have a casual role in some neoplasms in human beings. as examples, dna of hpv types 16, 18, and 31 are frequently present in genital cancers in humans. recently, oncogenic hpv types have also been identified in neoplasms of the head and neck, including verrucous carcinoma of the larynx, squamous cell carcinomas of the oral cavity and larynx, and inverted papillomas of the nose. these findings and our resource of an extensive panel of ...19911851275
absence of human papilloma virus in cervical adenocarcinoma determined by in situ hybridisation.a few studies using dna technology have suggested that human papillomavirus (hpv) may be an aetiological factor for adenocarcinoma of the uterine cervix. twenty one cases of cervical adenocarcinoma were studied by in situ hybridisation using biotinylated dna probes for hpv types 6, 11, 16 and 18 and a streptavidin, biotinylated alkaline phosphatase detection system. intranuclear hpv dna was detected in none of the adenocarcinomas, while positive controls gave a clear intranuclear signal. adjacen ...19911851501
human papillomavirus--associated vulvar verrucous carcinoma in a 20-year-old with an intact hymen. a case report.a 20-year-old woman with vulvar verrucous carcinoma, the youngest such patient reported to date, had human papillomavirus dna 6/11 identified with an in situ hybridization technique. in addition to her youth, the patient was unusual in that she had an intact hymen. although she denied having engaged in sexual activity, venereal transmission of the virus may have occurred without vaginal penetration.19911851516
prevalence of different types of human papillomavirus in cervical infection of north italian women.we investigated the prevalences of human papillomavirus (hpv) of type 6, 11, 16, 18, 31, 33, 35 and 42 in 276 cervical tissue samples biopsied from a group of north-italian women in which a hpv infection of the cervix had been suspected. the specimens were evaluated by conventional histological examination and by hpv typing, using southern blot hybridization on the extracted dna. we related different degrees of squamous cervical dysplasia, showing or not viral cytopathic effects, with the hpv ty ...19911851706
correlation of hpv antigen and type of genital warts with atypia.five clinical types of genital warts were identified, studied histopathologically and stained for human papilloma virus (hpv) antigen. hpv antigen was found in all degrees of koilocytosis and it was present more often in the hyperplastic warts than in other types of genital warts. however, as reported earlier, the presence of hpv antigen did not decrease proportionately as the degree of atypia increased.19911851721
human papillomavirus type 16 infection of the cervix: a comparison of differing dna detection modes and the use of monoclonal antibodies against the major capsid protein.an immuno-peroxidase technique using monoclonal antibodies against the major capsid protein l1 of hpv-16 was compared with dot-blot hybridisation of cervical scrapes and in situ hybridisation of cervical biopsy specimens for hpv-16 dna. in a series of 20 patients all techniques were specific for hpv-16 infection. ten patients were positive by dot-blot hybridisation and half of those were positive by in situ hybridisation. only one of hpv-16 dna positive cases showed l1 protein expression, appare ...19911851722
factors associated with clinical and sub-clinical anal human papillomavirus infection in homosexual men.(i) to determine the relative sensitivities of clinical examination, cytology and hpv dna hybridisation for the detection of anal human papillomavirus infection; and (ii) to examine various factors which may influence presentation of anal human papillomavirus infection in homosexual men.19911851723
probable nonpapillomavirus etiology of squamous cell carcinoma of the vulva in older women: a clinicopathologic study using in situ hybridization and polymerase chain reaction.a clinical, pathologic, and molecular virologic analysis of 30 cases of invasive squamous cell carcinoma of the vulva was undertaken to investigate the relationship of human papillomavirus (hpv) to this neoplasm. the presence of the virus was detected by the polymerase chain reaction and localized in the tumor and in the adjacent epithelium by in situ hybridization of paraffin sections of vulvectomy specimens. specimens were examined for nucleic acid sequences of hpvs 6, 11, 16, and 18 were dete ...19911851729
human papillomavirus screening in college women. 19911851770
in situ hybridization with human papillomavirus using biotinylated dna probes on archival cervical smears.we report a method of in situ hybridization (ish) of 10-year-old archival cervical smears with a cocktail of nick-translated human papillomavirus (hpv) dna types 6, 11, 16, 18, and 31. the method, which does not require destaining, results in excellent preservation of morphological detail with only 2% cell loss. methods of smear treatment and detection of the biotinylated probe with a multistep avidin-biotin-immunoperoxidase method are described. biotinylated pbr 322 plasmid and biotinylated hum ...19911851776
simultaneous detection and typing of genital human papillomavirus dna using the polymerase chain reaction.a simple method has been developed for detecting a broad range of genital human papillomavirus (hpv) types using the polymerase chain reaction (pcr). we utilized two consensus sequence primer pairs within the e6 and e7 open reading frames to amplify hpv dna; malignant hpv dna (from hpv-16, -18, -31, -33, -52b and -58) was amplified using the pu-1m/pu-2r primer pair whereas benign hpv dna (from hpv-6 and -11) was amplified using the pu-31b/pu-2r primer pair. identification of the amplification pr ...19911851805
an e1m--e2c fusion protein encoded by human papillomavirus type 11 is asequence-specific transcription repressor.we have isolated a putative, spliced e5 cdna of human papillomavirus type 11 (hpv-11) by polymerase chain reaction amplification of cdnas from an experimental condyloma. using retrovirus-mediated gene transfer, we isolated two novel hpv-11 cdnas, one of which had a splice linking nucleotides 1272 and 3377. this transcript also existed in experimental condylomata and in cervical carcinoma cells transfected with cloned genomic hpv-11 dnas. the 5' end of the transcript in transfected cells originat ...19911851879
very high incidence of germ cell tumorigenesis (seminomagenesis) in human papillomavirus type 16 transgenic mice.human papillomavirus type 16 (hpv16) is frequently found in carcinomas and precancerous lesions of the uterine cervix and is thought to be closely associated with carcinogenesis in these regions. however, the transforming activity of the e6 and e7 genes in vivo has not been characterized. to investigate this function, we produced transgenic mice carrying hpv16 e6 and e7 open reading frames. we obtained five transgenic founders and established three transgenic lineages. we observed testicular tum ...19911851881
rhesus papillomavirus type 1 cooperates with activated ras in transforming primary epithelial rat cells independent of dexamethasone.rhesus papillomavirus type 1 (rhpv-1) was recently cloned from a rhesus monkey lymph node metastasis of a penile squamous cell carcinoma. in this paper, we demonstrate that rhpv-1 cooperates with the activated ras oncogene to transform primary cells at a level comparable to human papillomavirus type 16. the viral dnas were cloned such that their expression was under the control of their natural promoter elements. unlike human papillomavirus type 16, rhpv-1 dna cooperated with ras independently o ...19911851883
the detection of genital human papillomavirus infection using polymerase chain reaction. 19911851893
tracheal papillomatosis. molecular diagnostic techniques and human papilloma virus. 19911851974
cervical human papillomavirus deoxyribonucleic acid and cytologic evaluations in gynecologic outpatients.we studied the prevalence and cytologic manifestations of human papillomavirus deoxyribonucleic acid among 2668 gynecologic outpatients. the study population consisted of 1573 women who came into the emergency room, 623 women from an abortion clinic who were seen because of induced first-trimester abortion, and 472 women who had been referred to a colposcopy clinic because of an abnormal papanicolaou smear finding. a dot blot technique was used for the detection of human papillomavirus types 6/1 ...19911852101
cloning of cdnas for cellular proteins that bind to the retinoblastoma gene product.the e7 transforming protein of human papilloma virus-16 binds to the retinoblastoma gene product (prb) through a nine-amino-acid segment of e7 (21-29). this segment of e7 is homologous to the prb-binding domains of the simian virus 40 large t and adenovirus e1a transforming proteins. each of these viral transforming proteins bind to the same region of prb. to isolate cellular proteins that interact with this viral protein-binding domain on prb, we used recombinant prb to screen a human complemen ...19911857421
amino-terminal domains of c-myc and n-myc proteins mediate binding to the retinoblastoma gene product.the proteins encoded by the myc gene family are involved in the control of cell proliferation and differentiation, and aberrant expression of myc proteins has been implicated in the genesis of a variety of neoplasms. in the carboxyl terminus, myc proteins have two domains that encode a basic domain/helix-loop-helix and a leucine zipper motif, respectively. these motifs are involved both in dna binding and in protein dimerization. in addition, myc protein family members share several regions of h ...19911865909
[multiple condylomata of the urethra and bladder disclosing hiv infection].the authors report the case of a 26 year old man, in whom recurrent hematuria revealed the diagnosis of urethral condylomata with bladder extension, and hiv serology positive. this case reminds us that it is essential to search for immunodeficiency in every case of extensive or recurrent condylomata. the analysis of such cases in the literature allows discussion of the various clinical courses according to the human papilloma virus types found in condylomatous tissues.19911867468
automated image-based cytometry with fluorescence-stained specimens.the combination of digitized microscopy, algorithms for object recognition and fluorescent labeling is a promising approach for reliable, quick, automated and cost-effective screening of clinical specimens. we describe two conceptually different algorithms for detecting objects in fluorescence microscopic images. one, which is partially automated, compares a mask that represents a typical object with every position in the image; the other, which is fully automated, calculates threshold intensiti ...19911867861
purification and characterization of human papillomavirus type 16 e7 protein with preferential binding capacity to the underphosphorylated form of retinoblastoma gene product.human papillomavirus type 16 e7 is considered to be a major viral oncoprotein playing an important role(s) in cervical cancers. e7 protein was shown to bind to the protein product of the retinoblastoma gene (rb), while simian virus 40 large t and adenovirus e1a were also shown to possess binding activity to rb protein. the rb protein is a cell cycle regulator that is highly phosphorylated specifically in s, g2, and m, whereas it is underphosphorylated in g0 and g1. recently, large t was demonstr ...19911870208
treatment failure of genital condylomata acuminata in women: role of the male sexual partner.the male sexual partners of women with genital condylomata acuminata are thought to be carriers of human papillomaviruses. it is therefore often recommended that both sexual partners be treated. we studied 360 women with genital warts to test the hypothesis that when the male partners of women with condylomata acuminata are treated, the treatment failure rate decreases. the male sexual partners of 180 of these women were examined and, if indicated, treated (treatment group). the 180 remaining ma ...19911872334
human papillomavirus infection and other risk factors for cervical neoplasia: a case-control study.a case control design has been used to investigate risk factors associated with the development of cervical squamous intraepithelial lesions (sil) in a population of urban women in which non-affluent minority groups were heavily represented. eighty-five women with histologically confirmed sil were compared to a control group of 70 cytologically normal women. hpv infection was determined using both southern blot hybridization and polymerase chain reaction (pcr) amplification specific for hpv type ...19911874571
hiv and other sexually transmitted diseases among female prostitutes in kinshasa.in 1988, 1233 prostitutes from different geographic areas of kinshasa participated in a cross-sectional survey on hiv infection and other sexually transmitted diseases (stds). despite relatively good knowledge about aids and stds, the reported preventive behaviour was poor. only 12% of the women reported regular use of condoms, while greater than 50% of the women reported regular use of antibiotics and 38% reported doing nothing specific to prevent stds. thirty-five per cent of the women were hi ...19911883543
cancer prevention: recent progress and future opportunities.knowledge gathered during the past few decades from the fields of cancer epidemiology, carcinogenesis, biochemistry, and molecular biology, much of which was communicated through the pages of the journal cancer research, provides powerful new strategies for cancer prevention. indeed, i believe that it is possible to draft a blueprint for a comprehensive approach to cancer prevention, to be used as we approach the 21st century. this plan includes: (a) expanded epidemiological and laboratory studi ...19911884384
[colposcopy of the vulva].a detailed colposcopy study of the cervix, vagina and vulva was conducted in 365 women referred for genital human papillomavirus (hpv) lesions, abnormal cervico-vaginal smear or cervical intraepithelial neoplasia (cin). valvuloscopic abnormalities, all biopsied, were found in 144 women, i.e. 40% of the population studied. five types of vulvoscopic abnormalities are described: diffuse acidophilia (55% of abnormalities), acidophilic maculae (17%), micropapillae (20%), papulae (4%) and leucoparaker ...19911885886
[cervico-vaginal cytology and condylomata. reproducibility and predictive value].human papilloma virus (hpv) is associated with two groups of intra-epithelial lesions of the cervix: the condyloma or the very low grade lesion, and intra-epithelial cervical neoplasia (cin) or a higher grade of lesion. cervico-vaginal cytology can be used to screen for these two types of lesion. further to this research is to evaluate the reproducibility and the predicted value of cervico-vaginal cytology to detect condylomas. the study is based on 610 patients who were referred because of abno ...19911885887
[spontaneous regression of flat condylomata of the cervix].in this study 146 patients with a human papilloma virus infection of the cervix detected on cervical smear. this infection was at the worst associated with cin 1 or cin 2. no treatment was carried out on these patients except for advising the use of condoms. among the 123 women whose diagnosis was cin 1, 65.2% of them had a negative smear within 18 months. this somewhat high rate of spontaneous regression was found in out patients who were not selected. they are near the results published by bro ...19911885888
endogenous hela p53 proteins are easily detected in hela cells transfected with mouse deletion mutant p53 gene.it has been reported [matlashewski et al. (1986). eur. j. biochem., 154, 665-672] that hela cells contain no detectable p53 protein, although they contain p53 mrna which is translationally active. here it is shown that endogenous hela p53 proteins were easily detected in hela cells transiently expressing mouse deletion mutant p53 gene after transfection with the appropriate recombinant plasmid. this detection was obtained by immunoprecipitation coupled with sds-page as well as by western blottin ...19911886712
subcutaneous interferon alpha 2a combined with cryotherapy vs cryotherapy alone in the treatment of primary anogenital warts: a randomised observer blind placebo controlled study.to compare patient tolerance and treatment efficacy of subcutaneous interferon (ifn) alpha 2a plus cryotherapy versus cryotherapy alone in treatment of primary anogenital (ag) warts.19911916791
presence of human papilloma virus type 16 dna sequences in human nonmelanoma skin cancers.the presence of human papillomaviruses (hpv) has been shown to be associated with the development and progression of invasive cancers of the genital tract, skin, and head and neck. in this study we analyzed 37 human nonmelanoma skin cancers (21 squamous cell carcinomas and 16 basal cell carcinomas) for the presence of hpv sequences. the polymerase chain reaction (pcr) was employed using primers designed to amplify dna encoding the e6-e7 region of hpv types 6b/11, 16, and 18. hpv type 6b/11 and 1 ...19911919051
clinical significance of hyperkeratosis on otherwise normal papanicolaou smears.although hyperkeratosis is a common cytological finding on papanicolaou smear, its clinical significance is unclear.19911919451
prevalence of hpv cervical infection in a family planning clinic determined by polymerase chain reaction and dot blot hybridisation.the overall prevalence of human papillomavirus (hpv) cervical infection in 131 women attending a family planning clinic was 7% (hpv 6/11, 16, 18, 31) by dot blot hybridisation, 53% (hpv 11, 16, 31) by polymerase chain reaction (pcr), and 56% by the two methods combined. hpv 16 and 18 were the commonest types (4% each) by dot blot, hpv 16 (39%) by pcr. fifteen percent of subjects had mildly abnormal cervical cytology (grades 1a, 2a, or 3). there was no significant correlation between cytological ...19911919537
human papillomavirus, gonorrhea, syphilis, and cervical dysplasia in jailed women.we assessed the prevalence of human papillomavirus (hpv) by cervicovaginal lavage and southern blot and inquired about behavioral risk factors for cervical disease and sexually transmitted diseases by interview in 114 female detainees at a large new york city jail. of the women screened, 8% had abnormal pap smears, 35% had hpv, 7% had gonorrhea, and 22% had serologic syphilis. given the high rates of hpv infection and cervical cytology, pap smears should be a routine intake procedure for incarce ...19911928533
an ounce of prevention ... stds and women's health.using recent data from the u.s., the argument is reiterated that sexually transmitted diseases (stds) have serious consequences on female morbidity, mortality, pregnancy outcome and that the cost of screening and treatment can afford significant savings. since the rosy view of the 1950s that stds had been eliminated with penicillin, new viral, parasitic and bacterial forms have emerged, and antibiotic-resistant classical stds have increased yearly. 13 million new cases were reported in 1989, e ...19911936219
warts: benign or malignant?the number of identifiable types of human papillomavirus (hpv), based on deoxyribonucleic acid (dna) sequence, has steadily increased in recent years. although at one time verruca vulgaris was considered a benign reactive proliferation, current evidence submits that this is not the case; some types of hpv are malignant.19911940290
squamous cell carcinoma of the scrotum: long-term followup of 14 patients.squamous cell carcinoma of the scrotum was diagnosed in 14 patients from 1945 to 1990. patient age at diagnosis ranged from 40 to 73 years, with the mean age of 62 years. the most common presentation was a solitary skin lesion but inguinal adenopathy was noted in 5 patients (36%). the mean delay to diagnosis for all patients was 22 months, with a range of 2 months to 10 years. predisposing factors included psoriasis treated with coal tar or arsenic, human papillomavirus infection and multiple cu ...19911942281
a novel nuclear inhibitor i-92 regulates dna binding activity of octamer binding protein p92 during the cell cycle.nuclear dna binding protein p92 is a sequence specific octamer binding protein with identical molecular weight as the ubiquitous octamer binding protein oct-1. it binds to octamer related sequences from the enhancer of human papillomavirus type 18. the activity and intracellular distribution of p92 is regulated by extracellular signals. in serum starved hela-fibroblast hybrid cells p92 is localized to the cytosol. serum stimulation leads to nuclear import of p92. in fractions of asynchronously g ...19911945850
[condylomata acuminata in childhood: description of a case].recent advances in understanding human papillomavirus imply that hpv infections are increasing in both men and women. hpv may cause subclinical disease and play a role in genital tumors. in prepuberal children the problem remains uncommon and can offer a difficult task of management and epidemiologic evaluation.19911946004
cervico-vaginal screening in an std clinic.from may 1986 to march 1988, there were 3,622 "new" female clients at the calgary sexually transmitted disease (std) clinic of whom 2,278 registered for the first time. a cervico-vaginal (pap) smear was obtained from those who had not had one in the previous 6 to 12 months and any history of venereal warts (vw) was recorded. 621 smears were accessed of which 611 were suitable for inclusion in this study. 65 (10.6%) smears revealed human papillomavirus (hpv) and/or cervical intraepithelial neopla ...19911954594
complications of cervical cryotherapy in adolescents.cervical dysplasia and human papillomavirus (hpv) infections in sexually active adolescents appear to be increasing. cervical cryotherapy offers the advantages of inexpensive outpatient therapy that is well tolerated without anesthesia. previous series have focused on women beyond adolescence and have concluded that the risks and complications are rare. sixty-seven adolescents underwent cervical cryotherapy for cervical intraepithelial neoplasia (cin) or hpv infection. pelvic inflammatory diseas ...19911956010
general sexually transmitted diseases.this review discusses current trends in sexually transmitted diseases. topics include treatment and prevention of syphilis and gonorrhea, as well as the association of sexually transmitted diseases with sexual assault. also discussed are the various organisms that cause vulvitis, including trichomonas, candida, and gardnerella species, along with a discussion of vulvar lesions that are especially difficult to diagnose and treat. diagnosis, sampling, pathogenesis, and treatment of chlamydia trach ...19911958806
clinical and pathological correlates of hpv type 16 dna in anal cancer.the aetiology of anal squamous cell carcinoma (scc) has recently been associated with a sexually transmissible agent--human papillomavirus (hpv) type 16. in this study clinical and pathological data from a prospective series of 67 anal scc collected over a three year period were compared with the hpv type 16 dna content of these tumours to determine whether any of the clinical or histological parameters might predict hpv dna content in a tumour specimen. of twelve clinicopathological variables e ...19901962812
[studies of human papillomavirus (hpv) in urological tumors].urological tumors were examined for the presence of human papillomavirus (hpv) dna by using southern blot hybridization. in 20 male patients with condyloma acuminatum, hpv type 6 was found at 85% (17/20), hpv type 11 at 95% (19/20), hpv type 16 at 5% (1/20) and hpv type 18 at 0% (0/20). in 2 female patients with condyloma acuminatum, hpv types 6, 11, 16 and 18 were found at 100% (2/2), 100% (2/2), 50% (1/2) and 0% (0/2), respectively. all 6 of the patients who were positive for hpv type 6, were ...19901962828
[in situ hybridization study of human papillomavirus from the penile cancer].the histochemical detection of human papillomavirus (hpv) was performed using the technique of in situ hybridization from the formalin fixed paraffin embedded specimens of penile carcinoma and condylomata+ acuminata . one of 19 penile cancer cases (5.3%) revealed the positive staining on the nuclei of the tumors for hpv type 16/18. however, the positive nuclei were scattered and localized in small part of the tumor which showed no koilocytosis. on the contrary, 11 of 12 condyloma acuminata cases ...19901962829
[analysis of human papillomavirus genome by pcr]. 19901962867
detection of human papillomavirus (hpv) type 16 and 52b in cervical cancer tissues by southern blot hybridization and polymerase chain reaction (pcr).dna samples from cancer tissues diagnosed histologically as squamous cell carcinoma of the uterine cervix were examined for the presence of human papillomavirus (hpv) genome dna by southern blot hybridization. of 63 specimens, 32 were found to hybridize with hpv dna probes; 23 specimens (35%) with hpv type 16 (hpv16), one (2%) with hpv type 18 (hpv18), four (7%) with hpv type 52b (hpv52b), and four others (7%) weakly with hpv52b. specimens negative for hpv dna with southern blot hybridization we ...19901962976
hybricomb. a novel diagnostic tool for dna probing.a technique is described in which unlabeled dna probes are immobilized on the plastic surface of a 12-tooth comb and used to capture homologs by a hybridization procedure. in the examples described, cellular dna from cervical biopsies is chemically labeled using the chemi-probe system. the sample containing labeled dna is then hybridized with the immobilized unlabeled probe (reverse hybridization). by means of this technique, human papillomavirus sequences can be detected with a sensitivity comp ...19901963055
transcriptional activation of the human papillomavirus-16 p97 promoter by an 88-nucleotide enhancer containing distinct cell-dependent and ap-1-responsive modules.the p97 promoter upstream of the oncogenic early genes of human papillomavirus (hpv)-16 is active in keratinocytes and in cervical carcinoma cells due to a 5' keratinocyte-dependent cis enhancer. in this study, we have mapped the main enhancer activity to an 88-nucleotide (nt) fragment composed of multiple cis elements. a 63-nt promoter-proximal enhancer core was sufficient for p97 activation in a human keratinocytic cell line, hacat, and in cervical carcinoma cells. although the enhancer functi ...19901963084
herpes simplex and human papillomavirus genital infections: controversy over obstetric management.plasma inhibitory factors, high levels of sex hormones, and depression of cell-mediated immunity may interfere with the natural host resistance to viral infections during pregnancy. it is apparent that hormonal, immunologic, and vascular changes in pregnancy may account for increased replication of herpes and for enhanced growth of condylomatous lesions. the challenge is to develop a rational plan of management for pregnant patients with herpes simplex or human papilloma virus infection. there h ...19901963124
discrimination of closely homologous hpv types by nonisotopic in situ hybridization: definition and derivation of tissue melting temperatures.it is generally assumed that nucleic acid association during in situ hybridization reactions is similar to that of nucleic acid association in solution. this assumption has been investigated by detecting closely homologous human papillomavirus types 6 and 11 by in situ hybridization as a model for the evaluation of stringency conditions in clinical biopsies. by examining matched and mismatched, labelled and target sequences under various stringency conditions, empirical dna-dna stability curves ...19901963174
study of sequences of dna of the human papilloma virus genotypes 6 and 16 in patients with cancer of vulva and cervix uteri.puntaction hybridization method was used to determine the incidence of the human papilloma virus infection in cases with the squamous cell cancer of the vulva and cervix uteri. the presence of sequences of dna of the hpv genotype and 16 was found in 74.2% of cases, which points to a suspicion for the involvement of the above viruses in etiology of cervix uteri cancer, as well as vulva cancer.19901963384
differential detection of human papillomavirus dna type 6 and 11 amplified by polymerase chain reaction.we have developed a method for differential detection of human papillomavirus type 6 and 11 with selective amplification of a segment of the viral dnas by the polymerase chain reaction. the target sequence for amplification was a 134-bp (bp 206-339) in the e6 open reading frame of hpv 6 and 11 dnas. dna extracted from a paraffin-section or a minute fresh biopsy specimen of condyloma acuminatum was amplified and hybridized with an oligonucleotide probe specific for hpv 6 or 11 dna. the method was ...19901963553
[relationship between human papillomavirus and oncogenes (c-myc, n-myc) amplification in human cervical cancers].the human papillomavirus detection and oncogenes amplifications were studied on dnas from fifteen cervical cancers. we detected hpv16 and hpv18 using southern blot hybridization and polymerase chain reaction (pcr) technique. the positive subjects of hpvs were eight cases (53%) observed by southern blot hybridization and fourteen cases (93%) by pcr technique. the gene amplifications of oncogenes (c-myc and n-myc) were analysed by slot-blot method and were observed in c-myc but not in n-myc. the " ...19901963632
human papillomavirus dna from warts for typing by endonuclease restriction patterns: purification by alkaline plasmid methods.the alkaline plasmid dna extraction method of birnboim and doly was applied for the isolation of human papillomavirus (hpv) from warts. tissue from common and plantar warts was digested with proteinase k, and the extrachromosomal circular covalently-closed form of hpv-dna was rapidly extracted by alkaline sodium dodecyl sulphate and phenol-chloroform treatment. recovery of hpv-dna from the tissue was sufficient for determination of endonuclease restriction patterns by agarose gel electrophoresis ...19901964033
treatment of latent rabbit and human papillomavirus infections with 9-(2-phosphonylmethoxy)ethylguanine (pmeg).the acyclic nucleotide pmeg was studied for effectiveness against shope papillomavirus (crpv) infection of rabbits and human papillomavirus type 11 (hpv-11) infections of human foreskin xenografts in athymic mice. pmeg given in the latent period strongly suppressed the subsequent growth rates of shope papillomas. pmeg starting in the latent period and continuing for the duration of the experiment, inhibited hpv-11 infections of human skin, including condyloma growth, and synthesis of viral dna a ...19901964372
precursor lesions of the cervix.screening has played a major role in decreasing the morbidity and mortality associated with carcinoma of the cervix. aggressive management of lesions recognized as precursor to invasive squamous carcinoma, (cervical intraepithelial neoplasia), has markedly reduced the incidence of invasive cancer. squamous carcinomas constitute most of the cervical cancers. the relationship between human papilloma virus and cervical abnormalities is reviewed. nursing diagnoses and care planning ideas are discuss ...19901964388
a comparative sequence analysis of two human papillomavirus (hpv) types 2a and 57.hpv 2a is commonly associated with verrucae vulgares, whereas hpv 57 was detected in mucosal lesions of the maxillary sinus and the genital tract, as well as in cutaneous lesions. the complete dna sequences of hpv 2a and hpv 57 were determined. the hpv 2a genome consists of 7860 base pairs and the hpv 57 genome contains 7861 base pairs. on the nucleotide level an 83% homology between the two sequences could be ascertained. compared to other hpvs they have a high g/c-content (hpv 2a: 48.8%, hpv 5 ...19901964523
detection of human papillomavirus (hpv) dna in oral squamous cell carcinomas by in situ hybridization and polymerase chain reaction.a series of 156 formalin-fixed, paraffin-embedded biopsies from 40 patients with surgically-treated oral squamous cell carcinomas was analysed for the presence of human papillomavirus (hpv) infection by histopathological evaluation, in situ dna hybridization and polymerase chain reaction (pcr). epithelial changes suggesting a hpv lesion within, or adjacent to, the carcinoma lesions were found in 16 out of 40 patients (40%). morphological signs of a flat hpv lesion were found in four cases (10%), ...19901964547
immunology of anogenital human papillomavirus (hpv) infection.a subgroup of human papillomaviruses (hpvs) is implicated in the aetiology of neoplastic change in anogenital epithelium. although abundant circumstantial evidence exists for the involvement of the immune system in the control of hpv infection, restriction of infection to epithelium and the lack of a viral productive phase pose problems for immune response induction, and for immunological effector mechanisms. in this article we discuss how hpv antigens may be presented to the immune system on th ...19901964551
the changing prevalence of cervical human papilloma virus infection.in order to determine the change in frequency of human papillomavirus infection (hpv) over a 10-year period, cervical smears from 1978 were retrieved, cytologically reviewed and compared with cervical smears taken in 1988. in this way, the slides were reviewed using the same diagnostic criteria. the prevalence of hpv infection in the population assessed at flinders medical centre, rose from 0.58% to 5.6% over this period.19901964552
human papillomavirus dna in unselected pregnant and non-pregnant women.human papillomavirus (hpv) dna is associated with genital squamous cell neoplasia, especially types 16, 18, and 31. several studies, using histopathology and dna hybridization, have shown an increased prevalence of cervical hpv dna in pregnant women. we determined the prevalence of hpv dna and the distribution of specific hpv dna types in exfoliated cervical cells from 748 randomly selected pregnant and 503 non-pregnant women, using a dot blot dna hybridization technique. the prevalence of hpv d ...19901965153
[penile lesions induced by human papillomavirus. an immunohistochemical study and dna hybridization].forty-nine male patients with clinically evident genital warts or suspected hpv-induced lesions detected by 5% acetic acid test were biopsied. typical cytological and structural hpv-induced changes were observed on histological examination in 37 of 49 patients. the immunohistochemical detection of hpv capsid antigen was positive in 15 of 45 cases. in situ hpv-dna hybridization was positive in 26 of 47 cases (24 hpv 6/11; 2 hpv 16/18). quick blot hpv-dna hybridization was positive in 20 of 28 cas ...19901965182
[vulvar pruriginous squamous papillomatosis].pruritic vulvar squamous papillomatosis (pvsp) is a recently described clinical entity whose aetiologic agent has been reported to be the human papilloma virus. we have seen 9 patients with pvsp. we believe that our clinical and histological data, along with the results of the in situ hybridizations stress out the evidence of a viral aetiology in the pvsp.19901965223
[cellular dyskeratosis as predictive factor in condylomatosis histologically confirmed by biopsy].human papillomavirus infection (hpv) causes cellular alterations which are detectable from a cervico-vaginal smear. cells marked by these alterations are defined as koilocytes and diskeratocytes. whereas koilocytes are pathognomonic of hpv infection, diskeratocytes, found in isolation on a smear, do not always play a reliable diagnostic role. in this study, a sample group of 82 women aged between 25 and 60 (mean age 44 years) were examined who, following cytological tests, revealed isolated or a ...19901965224
[pathognomonic signs of human papillomavirus infection in diagnostic material from the uterine cervix].etiological factors and mechanisms of pretumorous and tumourous processes in the female genitalia are presented in light of current concept of viral cocarcinogenesis. the utility of cytoscreening for the detection of the papilloma-virus infection in the uterine cervix mucosa is founded. detailed cytological characteristics of cells in the uterine cervix mucosa with a cytopathic effect (coilocytosis, the presence of two or many nuclei, vacuolisation of cytoplasm, nuclear atypia) are presented. a ...19901965392
prevalence of genital human papillomavirus infections in a mass-screened finnish female population aged 20-65 years.the results of the nationwide, population-based cervical cancer screening programme (organized by the finnish cancer society since early 1960s) were analysed to establish the prevalence figures (and their changes) for genital human papillomavirus (hpv) infections in an unselected finnish female population (aged between 20 and 65 years) screened in kuopio province between 1981 and 1989. during the study period 82,393 women were invited on a regular basis for the mass-screening, and also 4131 wome ...19901965568
detection of hpv-16 dna in cervical carcinoma by paraffin section in situ hybridization.thirty-eight cervical carcinomas and 1 cervical condyloma were examined for the presence of human papillomavirus (hpv) type 16 dna using 3h-dctp-labelled hpv-16 dna probe in paraffin section in situ hybridization and 32p-dctp-labelled probe in dot blot hybridization (tm-17). the results showed an hpv-16 dna positive rate in our series of about 74.4% (29/39) as determined by paraffin section in situ hybridization and about 71.8% (28/29) by dot blot hybridization. there was no significant differen ...19901965917
[misleading and rare form of vulvar carcinoma with fast development. suspicion of human papillomavirus infection]. 19901965999
genital human papillomavirus infection among women from major ethnic groups in singapore.the close epidemiological relationships between specific genital human papillomavirus (hpv) types and neoplasia of the cervix uteri have been extensively documented worldwide, including singapore. cervical cancer incidence rates in singapore show variations between the major ethnic groups. to ascertain the corresponding hpv infection rates among the various races in singapore, we analysed the cervical smears of 225 women by filter in situ dna hybridization, and compared the data with a previous ...19901966000
[pathomorphology of human papillomavirus (hpv) infection of the cervix uteri and vulva].an infection with human papilloma virus (hpv) is directly associated with the development of the cervical and vulvar carcinomas. the infection should be diagnosed and considered in the choice of therapy. taking no account of hpv infection in histological pictures may lead to overdiagnosis of ca"0" (cis). hpv infection is possible to be recognized--in most cases--in cytological and histopathological studies. cytological and histopathological studies are the basis for other examination methods. th ...19901966114
[immunoreactivity directed towards human papillomavirus (hpv) structural antigen in perianal epithelial proliferation in humans].immunoreactivity directed towards the presence of a structural antigen of papilloma virus (hpv) was studied in patients with benign epithelial proliferations in the perianal area, squamous cell and cloacogenic carcinomas of the rectum and anus, using dako serum kits. positive reaction was found in five of eleven pointed condylomas and in one squamous cell papilloma, which is in accordance with other reports. negative reaction in patients with squamous cell carcinomas may result from methodologic ...19901966115
a prospective study of human papillomavirus infection of the cervix.in a prospective study 42 women, diagnosed as having low grade cervical intraepithelial neoplasia (cin), made a total of 281 clinic visits over a 45 month period. at each visit, they were subjected to cytological and colposcopical examination and samples were taken for human papillomavirus (hpv) dna hybridization studies and for the detection of non-hpv infections. hpv types 16 and/or 18 were found in 25% of all the samples tested and these virus types were detected in five of six (83%) women wh ...19901966270
cryotherapy in the management of histologically diagnosed subclinical human papilloma virus (hpv) infection of the cervix.the aim of the study was to establish the efficacy of cryotherapy in the management of histologically diagnosed subclinical human papilloma virus (hpv) infection of the cervix. women with genital warts who had subclinical hpv infection of the cervix and no other sexually transmitted diseases were followed up at six weeks, 12 weeks, six months, 12 months and 18 months after treatment with cryotherapy. they came to the out-patient department of genito-urinary medicine (gum) at st luke's hospital, ...19901966312
implications of human papillomavirus in cervical and vulvar cancer. 19901966318
detection of human papillomavirus (hpv) in genital warts and carcinomas by dna in situ hybridization in chinese patients.a series of 51 genital biopsies from normal epithelium, condylomata acuminata, leucoplakia and squamous cell carcinoma from chinese male and female patients were analysed for the presence of human papillomavirus (hpv) types 6, 11, 16 and 18 by dna in situ hybridization. all of the nine genital condyloma acuminata were positive for hpv dna, in which hpv 6 was found in six cases, hpv 11 in two cases and hpv 18 in one case. twelve out of the 21 cases (57.1% of the total) of cervical squamous cell c ...19901966324
human papillomavirus infection in the cervix of pregnant females in hong kong.routine cytological examination and hpv-dna probing were performed on cervical cells obtained from 215 pregnant chinese females. the procedure utilized the specific binding of a mixture of complementary [32p]-labelled rna probes to membrane-bound target hpv dna types 6, 11, 16, 18, 31, 33 and 35. positive results for hpv-dna were obtained in 11/215 (5%) of the cases. the cervical smears were routinely processed and in 3/215 (1.5%) cases a cytological diagnosis of wart virus infection was made. a ...19901966325
human papillomavirus oncoproteins.human papillomaviruses (hpvs) appear to play a role in the etiology of the vast majority of virus-associated human malignancies. studies of viral gene expression in carcinomas suggest the importance of two hpv encoded proteins, e6 and e7, in malignant development and these proteins have been shown to encode transforming and immortalising activities. the two proteins show some functional resemblance to the transforming proteins of other small dna tumour viruses such as adenovirus and sv40. recent ...19901966493
comparison between the polymerase chain reaction and slot blot hybridization for the detection of hpv sequences in cervical scrapes.sixty-four women presenting with a single mildly abnormal smear were investigated for infection with human papillomavirus (hpv) type 16 using both slot blot hybridization and polymerase chain reaction (pcr) amplification. pcr was nearly three times more sensitive for the detection of hpv 16 dna than slot blot hybridization. hpv 16 was not significantly associated with a risk of progression to cin if pcr was used to screen for infection. women who smoked were at significantly greater risk of prog ...19901966674
[esophageal lesions caused by human papillomaviruses (hpv)].twenty two patients are presented with histologic lesions on esophageal biopsy compatible with infection by human papillomavirus. symptoms were uncharacteristic, there was no sex difference and the mean age was 40 years. upper gi endoscopy showed the following: a) multiple ulcers in the middle esophagus, most of less than 10 mm in diameter, rounded or oval in shape, separated by normal appearing mucosa, well delimited erythematous margins, with a smooth white base; b) in five patients, multiple ...19901967089
occurrence of human papillomavirus dna types 16 and 18 (hpv-16/18) in cervical smears as compared to cytological findings.cervical smears from 2336 women were examined for the presence of hpv-16/18 by dot-blot hybridization using 32p-labeled hpv-16/18 dna under high stringency conditions. the hybridization data were compared with cytological findings classified according to papanicolaou. the ages of the patients ranged from under 20 to over 70 years. ninety-eight (4.4%) of the 2237 cytologically normal cervical samples (pap i and ii) were hpv-16/18 positive. thirteen out of 32 (40.6%) samples showing signs of mild ...19901968861
looking for human papillomavirus type 16 by pcr. 19901969096
association between poor prognosis in early-stage invasive cervical carcinomas and non-detection of hpv dna.human papilloma virus (hpv) dna sequences (hpv types 16, 18, 33, 35 or uncharacterized) were detected by southern blot hybridisation and polymerase chain reaction in 84% of 106 early-stage invasive carcinomas of the uterine cervix. among hpv-positive patients, the risk of overall relapse did not differ with individual hpv types. compared with hpv-positive patients, those with no detectable hpv dna had a 2.6 times higher risk of overall relapse (p less than 0.05) and 4.5 times higher risk of dist ...19901971033
anti-contamination primers to improve specificity of polymerase chain reaction in human papillomavirus screening. 19901971367
anal human papillomavirus infection among human immunodeficiency virus-seropositive and -seronegative men.homosexual men are at high risk of anorectal human papillomavirus (hpv) infection, hpv-related anal cancer, and precancer, conditions known to increase with immunosuppression. the relationship between anal hpv infection, human immunodeficiency virus (hiv) infection, and immunosuppression was studied in homosexual men seen at a sexually transmitted disease clinic. history or presence of warts on rectal examination, and detection of anorectal hpv dna were each significantly associated with hiv ser ...19901973695
detection of human papillomavirus (hpv) dna in laryngeal papilloma.laryngeal papillomas are troublesome benign tumors of the larynx. according to their biological behavior, there are two types of laryngeal papillomas--the juvenile and adult types. two samples of adult-type and five samples of juvenile-type laryngeal papilloma specimens were studied using the southern blot technique with radioactively labeled human papillomavirus (hpv) 6b, 16, 18 deoxyribonucleic acid (dna) as probes. we found that there was a linear form, an episomal form, and a concatemer or c ...19901976746
malignant transformation of condyloma acuminatum: report of a case.condyloma acuminatum is a benign warty lesion, whose malignant change is rarely seen. a 69-year-old woman with a large vulvar condyloma acuminatum, which she had had for about 14 years, underwent a vulvectomy, and a malignant component to the lesion was found unexpectedly. the continuity from a benign to a malignant lesion was evident in the histopathologic examination. infection with human papillomavirus type 6 was documented by dna hybridization. the use of a one-step reverse dot blot hybridiz ...19901977864
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