Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| validation of virus inactivation by heat treatment in the manufacture of diaspirin crosslinked hemoglobin. | diaspirin crosslinked hemoglobin (dclhb), a hemoglobin based oxygen carrying solution prepared from outdated human blood, is subjected to a heat treatment step to inactivate viruses in our manufacturing process. to validate the efficacy of this inactivation, we have simulated the heat treatment procedure at a reduced scale using hemoglobin solution spiked with representative viruses. human immuno-deficiency virus (hiv), cytomegalovirus (cmv), and duck hepatitis b virus (dhbv) were used in this v ... | 1992 | 1327242 |
| [expression of hepatitis b virus x protein in tumor and nontumor tissues of patients with hepatocellular carcinoma (hcc)]. | pap technique and rabbit anti-x serum were used to detect the x protein in tumor and nontumor liver tissues from 34 patients with hcc. the positive rate of the x protein in both tissues were 94.1% and 84.4% respectively. of the 34 patients with hcc, 27 were complicated by liver cirrhosis, in whom 92.6% were x protein positive in liver cells. it was found that almost all of the liver cells adjacent to the tumor tissue showed strong positive staining. the high frequency and predominant expression ... | 1992 | 1327450 |
| hepatitis c virus infection in hepatocellular carcinoma. detection of plus-strand and minus-strand viral rna. | although serum antibody to hepatitis c virus (anti-hcv) is found in many patients with hepatocellular carcinoma, the actual roles of hcv in carcinogenesis are unknown. | 1992 | 1327494 |
| duck hepatitis b virus infection and duck hepatocellular carcinoma. | to study the relationship between duck hepatitis b virus (dhbv) infection and duck hepatocellular carcinoma (dhcc), histological examination and dhbv dna hybridization were performed in 875 ducks from three flocks in qidong county. among them, 34 suffered from hepatoma, including 23 hepatocellular carcinoma, 8 cholangiocarcinoma and 3 hepatocellular-cholangiocarcinoma. of the 34 ducks with hepatoma 27 were positive for dhbv dna in the liver and/or serum. dhbv dna was demonstrated in neoplastic n ... | 1992 | 1327668 |
| [detection of hepatitis b virus dna in leucocytes of primary hepatocarcinoma using polymerase chain reaction]. | the detection of hbv dna in sera and leucocytes of 30 primary hepatocarcinoma patients whom were negative for hbsag by reverse passive hemagglutination using polymerase chain reaction (pcr) were reported. the detectable rates of hbv dna in leucocytes and sera were 86.7% and 70% respectively and the serological indexes, 43.4%. these indicated that hbv dna may existent in blood of the patients with negative serological indexes. it was common that hbv dna presented in peripheral leucocytes of prima ... | 1992 | 1327673 |
| hcv rna and antibody to hcv core protein in japanese patients with chronic liver disease. | we evaluated the prevalence of hepatitis c virus (hcv) rna and antibody (anti-hcvcore) to the putative hcv core protein in japanese patients with chronic liver disease. sera were screened by solid-phase enzyme immunoassay with a recombinant hcv core protein and by the reverse transcription-polymerase chain reaction (rt-pcr) test which directly detects the hcv genome. anti-hcv core was detected with high titers in 95% (69/73) of chronic non-a, non-b hepatitis, and 94% (65/69) of anti-hcvcore-posi ... | 1992 | 1327684 |
| hepatitis c and b viruses in hepatitis b surface antigen-negative hepatocellular carcinoma. | the relative role of hepatitis c virus and hepatitis b virus in hepatitis b surface antigen-negative hepatocellular carcinoma was evaluated by polymerase chain reaction in 31 patients from taiwan. twenty-one were positive for antibody to hepatitis c virus (group 1) and 10 were negative (group 2). of the group 1 patients, hepatitis c viral rna was detected in the serum by polymerase chain reaction in 16 and in the liver tissue in 17, whereas hepatitis b viral dna was found in the liver tissue in ... | 1992 | 1327934 |
| resistance of hepatitis delta virus replication to interferon-alpha treatment in transfected human cells. | interferon-alpha (ifn-alpha) is known to inhibit both dna and rna viruses, including hepatitis b virus (hbv). in humans the antiviral effect, if any, of ifn-alpha on hepatitis delta virus (hdv) is complicated by the fact that hdv is spread only to patients already infected with hbv. an in vitro model system was used to assay for an antiviral effect of ifn-alpha on hdv genome replication. hep g2 cells were transfected with a plasmid containing a trimer of hdv and treated with ifn (20 or 100 units ... | 1992 | 1328404 |
| type i interferons inhibit hepatitis b virus replication and induce hepatocellular gene expression in cultured liver cells. | a hepatoblastoma cell line transfected with hepatitis b virus (hbv) dna (hep g2.2.15) was used to investigate the effects of interferons (ifns) on hbv replication and hepatocellular gene expression. ifn-alpha 2b or -beta inhibited hbv replication transiently. in parallel, there was a decrease in the amount of hbv mrna. hepatitis b surface antigen and early antigen secretion were not influenced; however, their intracellular levels diminished during treatment. the cellular 2',5'-oligoadenylate syn ... | 1992 | 1328410 |
| replication of a mutant hepatitis b virus with a fused x-c reading frame in hepatoma cells. | we have previously described a mutant hepatitis b virus (hbv) with a fused x-c open reading frame (orf) resulting from a single nucleotide insertion in the x-c overlapping region. a stably transformed cell line producing hbv particles, hepg2-k8, was established by transfecting the human hepatoma cell line hepg2 with a plasmid carrying four tandem repeats of the mutant hbv genome. the virus particles secreted into the culture medium were characterized by density gradient centrifugation and electr ... | 1992 | 1328498 |
| increased expression of transforming growth factor alpha after transfection of a human hepatoblastoma cell line with the hepatitis b virus. | the expression of transforming growth factor alpha (tgf-alpha) was examined in a human hepatoblastoma cell line, hep g2, which does not contain hepatitis b virus (hbv) dna, and in the cell line 2.2.15, which was formed by the transfection of hep g2 cells with the complete hbv dna, to study the possibility that hbv and tgf-alpha could function as co-factors in hepatocarcinogenesis. northern blot hybridization of rna extracted from these cell lines, with densitometric analysis, revealed expression ... | 1992 | 1328501 |
| retinoid x receptor rxr alpha binds to and trans-activates the hepatitis b virus enhancer. | a retinoid x receptor (rxr) response element was located within the functionally defined hepatitis b virus (hbv) enhancer element. a short segment of the enhancer that contains this region has been shown with genetic analysis to play a key role in the regulation of enhancer function and to represent a major determinant of liver-specific activity. both the full-length protein and the dna-binding domain of the liver-specific receptor rxr alpha bound to the putative retinoic acid response element i ... | 1992 | 1329088 |
| blood donor screening or 'over-screening': how far to go in avoiding transmission of infectious agents? | 1992 | 1329352 | |
| hepatitis b virus dna integration and expression of an erb b-like gene in human hepatocellular carcinoma. | southern blot studies on hepatitis b virus (hbv) dna integration in 13 human hepatocellular carcinomas (hccs) patients revealed the presence of several distinct hbv integration sites in different human liver disease patients. in one hcc patient the dna fragment containing the hbv integration also hybridized to an erb b probe. the erb b/hbv co-migrating dna fragment was cloned and sequenced, and showed that hbv dna is integrated next to a cellular dna fragment which is homologous to the tyrosine ... | 1992 | 1329747 |
| lichen planus, liver kidney microsomal (lkm1) antibodies and hepatitis c virus antibodies. | no anti-liver kidney microsomal (lkm1) antibodies were detected in 46 patients with lp, 16 of whom had also a chronic liver disease (cld). in contrast, anti-hepatitis c virus (hcv) antibodies were found in 10% of patients with lp and in 50% of those with lp and cld. anti-hcv antibodies may be considered as a false-positive reaction in 56% of cases, especially when anti-lkm1 antibodies are present. our findings do not support such a hypothesis, but suggest that cld in lp patients is, at least in ... | 1992 | 1330094 |
| indication of liver transplantation for hepatocellular carcinoma in japan. | approximately 20,000 patients die of hepatocellular carcinoma (hcc) annually in japan and most of them are hepatitis b virus (hbv) or hepatitis c virus (hcv) carriers. recently, small hcc, less than 3 cm in diameter, have frequently been found by ultrasonography in the follow-up of patients with chronic liver diseases. such cases are mainly treated by either surgical resection or percutaneous ethanol injection therapy (peit) with a satisfactory 5 year survival rate of 50%. in addition, the survi ... | 1992 | 1330113 |
| interaction between alcohol consumption and positivity for antibodies to hepatitis c virus on the risk of liver cirrhosis: a case-control study. provincial group for the study of chronic liver disease. | to assess the risk of developing liver cirrhosis associated with alcohol consumption, hbv and hcv infection markers, we carried out a case-control study involving 115 patients admitted to the medical departments of the general hospitals in the province of l'aquila (abruzzo, italy) who received for the first time the diagnosis of liver cirrhosis, and 167 controls randomly selected among patients admitted to the same hospitals as the cases. alcohol intake was measured in all 282 subjects using an ... | 1992 | 1330673 |
| infection by hepatitis b and c virus in non-intravenous drug using female prostitutes in spain. | we have studied the prevalence of hepatitis b (hbv) and hepatitis c virus (hcv) serologic markers in female blood donors and in female prostitutes and the relationship of antibodies to hepatitis b core antigen (anti-hbc) and of antibodies to hcv (anti-hcv) with the presence of treponemal antibodies (fta-abs) in non-intravenous drug using female prostitutes. hepatitis b surface antigen (hbsag) was found in 1.0% of the female blood donors, anti-hbc in 15.6% and anti-hcv in 0.7%. in the prostitutes ... | 1992 | 1330674 |
| hepatitis type c virus infection in patients with type b chronic liver disease. | anti-c100-3 (ortho) was determined in the sera of 152 patients with hbs antigen-positive chronic liver diseases to assess coinfection of hepatitis b virus (hbv) and hepatitis c virus (hcv). eleven patients (7.2%) were positive for anti-c100-3. anti-cp-9 (okamoto) and hcv-rna (rt-pcr) were also examined in these 11 patients. anti-cp-9 was detected in 7 patients and hcv-rna was detected in all 11 patients. four of the 11 anti-c100-3-positive patients were positive for hbe antigen (hbeag) and other ... | 1992 | 1330796 |
| anti-hepatitis c virus in the elderly: a seroepidemiological study in a home for the aged. | a seroepidemiological survey of anti-hepatitis c virus (anti-hcv) was carried out in 315 institutionalized elderly people. hbv serum markers were tested in the same sera. clinical details were also studied in the anti-hcv-positive subjects. the overall prevalence of anti-hcv was 2.2%, while the prevalence of hbv serum markers was 36.8% (the hbsag prevalence was 0.6%). in 1 subject anti-hcv was found in association with hbsag positivity. serum transaminase levels were found within the normal rang ... | 1992 | 1330832 |
| biochemical pharmacology of (+)- and (-)-2',3'-dideoxy-3'-thiacytidine as anti-hepatitis b virus agents. | 2',3'-dideoxy-3'-thiacytidine (cis-(+/-)-sddc) was found to have potent activity against hepatitis b virus and human immunodeficiency viruses in culture. recent studies by us identified (-)-sddc as the stereoisomer responsible for the antiviral effect and showed that the cytotoxicity was mainly caused by (+)-sddc. metabolism studies showed that these drugs were converted to their monophosphates, diphosphates, and triphosphates. the enzyme responsible for the formation of monophosphates was ident ... | 1992 | 1331054 |
| improved serodiagnosis of chronic hepatitis c in japan by a second-generation enzyme-linked immunosorbent assay. | to clarify the role of hepatitis c virus (hcv) infection in chronic liver disease, sera from japanese patients which were negative by the original anti-hcv assay (ortho) were subjected to a second-generation anti-hcv assay based on a combination of structural (c22) and nonstructural (c200) recombinant hcv proteins. of 29 patients with chronic non-a, non-b hepatitis, 20 (69%) were anti-hcv-positive by the second-generation enzyme-linked immunosorbent assay (elisa) and also positive by the reverse ... | 1992 | 1331314 |
| regulation of transcription from the hepatitis b virus major surface antigen promoter by the sp1 transcription factor. | the dna-binding proteins which recognize the regulatory sequence elements of the hepatitis b virus (hbv) major surface antigen promoter were examined by gel retardation analysis, using nuclear extracts from the human hepatoma cell line huh7. using this assay, we identified four regions (b, d, e, and f) of the promoter that interact with the same or similar transcription factor(s). in addition, the recognition sequence for the sp1 transcription factor bound the same or similar transcription facto ... | 1992 | 1331502 |
| repression of liver-specific hepatitis b virus enhancer 2 activity by adenovirus e1a proteins. | two regions of the hepatitis b virus (hbv) genome have been shown to display properties of a transcriptional enhancer. enhancer 1 is active in most hepatoma lines examined as well as in some non-hepatocyte-derived cell lines. in contrast, enhancer 2 activity is strictly liver specific. in this study, we show that adenovirus e1a expression in the highly differentiated human hepatoma line huh6 strongly inhibits hbv enhancer 2-stimulated transcription while having no effect on hbv enhancer 1 activi ... | 1992 | 1331530 |
| [fundamental studies of hepatitis c virus: a review]. | in 1974, prince et al. reported the existence of posttransfusion hepatitis with a long incubation period which was not related to hepatitis b virus (hbv). these cases were named "non-a, non-b" (nanb) hepatitis. the genome of nanb hepatitis virus was discovered recently using a recombinant complementary dna (cdna) approach. it was termed the hepatitis c virus (hcv), and a specific diagnostic tool for the circulating hcv antibody (anti-hcv) was developed using a purified viral polypeptide derived ... | 1992 | 1331574 |
| [epidemiological study of hepatitis b and c virus in okinawa and kyushu, japan]. | epidemiological studies of hepatitis b virus (hbv) and hepatitis c virus (hcv) infection in okinawa and kyushu have been conducted since 1970. early in the study period, the prevalence of hbsag on iriomote and hateruma islands in okinawa was 9.0% and 6.1%, respectively, which were significantly higher than the 2.4% in kyushu. prevalence of anti-hbc on iriomote island was 59.8% and that on hateruma island 70.9%, also which were significantly higher than the 30.9% in kyushu. prevalence of hbv infe ... | 1992 | 1331575 |
| [cirrhosis and cirrhogenic diseases in tunisian children. multicenter study of 65 cases]. | over a period of 10 years, 65 cases of hepatic cirrhosis and cirrhogenic disease have been observed in five pediatric centers in mid tunisia. the age of the patients ranged from 30 days to 14 years. the main etiology was biliary cirrhosis (24 cases) followed by post-hepatic cirrhosis (15 cases). eight cases had a metabolic origin, which was wilson's diseases in five cases. three children had cirrhosis of a pre-hepatic origin. in 15 children, the liver biopsy showed the presence of cirrhosis but ... | 1992 | 1331967 |
| [practical remarks regarding the analysis of markers of acute viral hepatitis]. | 1992 | 1332002 | |
| [viral hepatitis a and b]. | 1992 | 1332266 | |
| clearance of hbsag in seven patients with chronic hepatitis b. | the natural history of chronic hepatitis b patients who spontaneously cleared serum hbsag was investigated. a total of 351 patients with chronic hepatitis b were observed in our hospital for at least 3 yr. seven of these patients became hbsag negative during the follow-up period. hbsag disappeared within 6 mo (range = 11 to 169 days, mean = 70 days) after acute elevation of alt. alt levels as high as 500 iu were found in three patients, whereas such elevation was not demonstrated in the other fo ... | 1992 | 1332920 |
| analysis of the p53 tumor-suppressor gene in hepatocellular carcinomas from britain. | human hepatocellular carcinomas from patients in britain, an area of low prevalence of hepatocellular carcinoma and low dietary exposure to aflatoxin b1, were analyzed for mutations in the p53 tumor-suppressor gene. abnormalities in the p53 gene were detected in 2 of 19 hepatocellular carcinomas by polymerase chain reaction--single-stranded conformation polymorphism. direct sequencing of the evolutionarily conserved regions of p53 (exons 5, 6, 7 and 8), where mutations have been commonly found i ... | 1992 | 1332921 |
| hepatitis b x-gene expression in hepatocellular carcinoma. | a rna-pcr method and different sets of primers were used to investigate the expression of different hepatitis b (hb) virus genes at the rna level. we tested paired samples (tumor and non-tumor) from the liver tissues of 48 taiwanese patients with primary hepatocellular carcinoma (hcc). by using a set of primers which spanned the sequences of the s-gene, we found expression in only 2 patients. in one hbs-rna was only detected in the tumor tissue and in the other only in the surrounding non-tumor ... | 1992 | 1333002 |
| in situ hybridization in viral hepatitis. | in situ hybridization (ish) is a sensitive and specific technique for detecting nucleic acids in intact cells. visualization of the target sequences by autoradiography or immunohistochemistry allows their precise subcellular localization and quantitation. the application of ish techniques has contributed to the understanding of the complex replicative cycle of hepatitis b virus. more recently, hepatitis delta and c virus replication has also been studied by this technique. ish-based assays have ... | 1992 | 1333029 |
| deletion and insertion mutants of hbsag particles. | we have found previously that hybrid 22-nm hbsag particles can be created by insertion of short antigenic sequences into the hbv major envelope protein. we have now performed a detailed deletion mutagenesis of the s gene of hbv encoding hbsag. deletion of the 51 c-terminal amino acids including most of the third and all of the fourth hydrophobic domain of the s protein did not affect particle assembly and secretion. however, secretion of 22-nm particles was abolished by minor deletions in the n- ... | 1992 | 1333319 |
| hepatitis in pre-school children: prevalent role of cytomegalovirus. | virological and serological investigations were performed on 8 children with clinical and/or laboratory signs of hepatitis. cytomegalovirus (cmv) appeared as the most frequently involved etiologic agent, since it was associated with 5 severe or chronically-evolving cases. out of the other 3 patients with non-cmv associated hepatitis, all completely recovering, two had clinically typical epstein-barr virus infections, while the remaining patient had an asymptomatic hbv infection. | 1992 | 1333326 |
| prevalence of antibody to hepatitis c virus in chronic hbsag carriers. | the presence of the anti-hcv antibody was investigated in sera from 102 chronic hbsag carriers. the subjects varied as to the characteristics of the clinical states. it was found that hcv coinfection was more common in hbsag positive intravenous drug addicts than in other parenteral risk groups. it also appears that hcv may be the causative agent of chronic liver disease in hbsag carriers with undetectable hbv (and possibly hdv) replication. | 1992 | 1333329 |
| replication of a molecularly cloned hbeag negative hepatitis b virus variant in transfected hepg2 cells. | 1992 | 1333333 | |
| [liver cancer and hepatitis b and c virus]. | chronic infection by hepatitis b and c viruses is frequently associated to the development of primary liver cancer. liver cirrhosis, induced by these viral infection, plays an important role in the liver carcinogenesis. in addition, hbv has a direct role in liver cell transformation by a transactivating effect of some viral proteins as well as insertional mutagenesis. the role of hepatitis c virus is not known. the strong association, even in france, of primary liver cancer to these viral infect ... | 1992 | 1333632 |
| non-radioisotopic methods for dna probes. | we have recently developed a new colorimetric method, dna enzyme immunoassay (deia), for detecting specific hybrids of complementary nucleic acids. this technology is based on an antibody that selectively recognizes double, but not single-stranded dna. the molecule does not react with a specific probe immobilized on microwells through an avidin-biotin bridge, nor with non-specific amplified sequences, since they are removed by washing. the antibody reveals the hybridization event, independently ... | 1992 | 1333735 |
| hepatitis b virus biology and pathogenesis. | 1992 | 1333869 | |
| clinical aspects and epidemiology of hepatitis b and c viruses in hepatocellular carcinoma in japan. | the incidence of hepatocellular carcinoma (hcc) in japan has increased over the past two decades. of the 379 patients with hcc treated at shinshu university hospital over the past 20 years, 112 underwent treatment between 1971 and 1980 and 267 were treated between 1981 and 1990. the prevalence of hepatitis b virus-associated hcc and hepatitis c virus-associated hcc was 54% and 34%, respectively, during the first decade and 31% and 60%, respectively, during the second decade. major factors contri ... | 1992 | 1333901 |
| cells transfected with human interleukin 6 cdna acquire binding sites for the hepatitis b virus envelope protein. | earlier studies revealed that human interleukin 6 (il-6) contains recognition sites for the hepatitis b virus (hbv) envelope (env) protein, and that il-6 and anti-il-6 antibodies, respectively, inhibited the interaction of cells expressing a receptor for hbv with the pres(21-47) segment of the hbv env protein, encompassing the complementary attachment site for il-6. this suggested that il-6 mediates hbv-cell interactions. we report that: (a) chinese hamster ovary cells transfected with human il- ... | 1992 | 1334115 |
| detection of hepatitis c viraemia in caucasian patients with hepatocellular carcinoma. | potential risk factors for the development of hepatocellular carcinoma were analysed in 40 caucasian patients with this malignancy. a higher proportion (14 of 40; 35%) had evidence of hepatitis c virus (hcv) infection than had evidence of either hepatitis b virus (hbv) carriage (17.5%) or alcohol abuse (30%). in all 14 patients whose sera were reactive by hcv elisa (ortho second generation test), the presence of antibodies to hcv were confirmed by recombinant immunoblot assay (ortho riba-2). fur ... | 1992 | 1334130 |
| antibodies to a putative hepatitis c virus polyprotein in japanese patients with chronic liver disease. | to investigate the frequency of exposure to hepatitis c virus (hcv) in chronic liver disease, sera from japanese patients were tested with the original anti-hcv assay (ortho) and an anti-hcv assay based on synthetic peptides corresponding to a variety of regions in the hcv genome. thirty-one (67%) of 46 patients with chronic non-a,non-b hepatitis were anti-hcv-positive by the ortho elisa, 20 of whom were also positive by elisa based on synthetic hcv peptides. eight (53%) of the 15 patients negat ... | 1992 | 1334132 |
| age at first establishment of chronic hepatitis b virus infection and hepatocellular carcinoma risk. a birth order study. | it is frequently assumed that the risk of hepatocellular carcinoma related to hepatitis b virus is higher when chronic hepatitis b virus infection is acquired early in life. this hypothesis has never been directly evaluated. however, firstborn and secondborn children are exposed to common infections after their school enrollment, whereas laterborn children are exposed much earlier, through their older siblings. the authors analyzed sibship size and birth order data from a large case-control stud ... | 1992 | 1334366 |
| persistent hepatitis b virus infection and hepatoma in the gambia, west africa. a case-control study of 140 adults and their 603 family contacts. | to determine the incidence of persistent hepatitis b virus infection and its etiologic role as a cause of hepatoma, the authors carried out a case-control investigation of 70 persons with hepatoma, 70 controls, and their families in 1981-1982 in the gambia, west africa. the risk of developing hepatoma after the age of 39 years was 1.4% in men and 0.3% in women. hepatoma occurred more than twice as frequently among persons who had four or more older siblings as among persons who had less than two ... | 1992 | 1334367 |
| detection of replicative hepatitis c virus sequences in hepatocellular carcinoma. | several serologic studies suggest that infection by hepatitis c virus (hcv) may be associated with the development of hepatocellular carcinoma (hcc). therefore, we examined tumor tissue and/or the surrounding liver of 20 patients for viral sequences by the polymerase chain reaction (pcr). in 12 cases, liver and tumor tissues were separable for extraction. rna was extracted from frozen tissues and used as a template for reverse transcription followed by double pcr with nested primers for the 5'-u ... | 1992 | 1334635 |
| [the circulation of the hepatitis a and b viruses in the somali population]. | we report the results of a study carried out to evaluate the extent of hepatitis a virus (hav) and hepatitis b virus (hbv) circulation in somalia. serum samples were collected from 593 subjects (age range 0-83 years) and tested for anti-hepatitis a (hav) and anti-hav igm. serum samples taken from 1272 individuals (age range 0-83 years) were tested for hbsag, anti-hbsag, anti-hbcag, hbeag and anti-hbeag. we confirmed a very high rate of hav exposure (about 90% of the subjects tested had circulati ... | 1992 | 1334688 |
| expression of oncogenes and tumor suppressor genes in human hepatocellular carcinoma and hepatoblastoma cell lines. | the expression of nine oncogenes (c-myc, n-myc, n-ras, h-ras, k-ras, abl, fos, src, and raf) and two tumor suppressor genes (p53 and rb) were studied by northern blot hybridization in six human hepatocellular carcinoma or hepatoblastoma cell lines (plc/prf/5, hep3b, hep g2, 2.2.15, hle, and hlf) and in a human embryonic lung fibroblast cell line (wi-38) to look for differences that might be associated with the presence (plc/prf/5, hep3b, and 2.2.15) or absence (hep g2, hle, and hlf) of integrate ... | 1992 | 1335479 |
| mammalian hepatitis b viruses and primary liver cancer. | hepatitis b virus is a major etiologic agent in the development of human hepatocellular carcinoma, but the precise role of the virus in the tumorigenic process is still unclear. recent studies of naturally occurring animal models, such as woodchucks and squirrels infected with hepatitis b-like viruses (hepadnaviruses) have revealed different oncogenic strategies and outlined the predominant role of myc genes in rodent hepatomas. higher oncogenicity of woodchuck hepatitis virus has been correlate ... | 1992 | 1335794 |
| sixty cases of primary hepatocellular carcinoma in one year. a preliminary appraisal. | we studied all the 70 cases of liver cancer referred to us in 1988. sixty (85.71%) were primary hepatocellular carcinoma (hcc), the other 10 (14.29%) were metastatic liver cancer. of the 60 hcc, 48 were males and 12 females. peak age incidence was between 20-40 years. forty-five (75%) had tumours in both lobes. of the remaining 15, seven had tumours in the right lobe while eight were in the left. more detailed assessment identified eight as child's c. seven (11.66%) had laparotomy, and two were ... | 1992 | 1336000 |
| recent advances in the study of hepatocellular carcinoma. | among the known causes for the occurrence of hepatocellular carcinoma (hcc), chemical carcinogens, chronic alcoholic intake and hepatitis b virus (hbv), especially in asia, has been emphasized. hbv has been industriously studied and many queries about the relationship between hbv infection and hepatocarcinogenesis have been clarified. recent discovery of hepatitis c virus (hcv) revealed that there may be the participation of this virus in hepatocarcinogenesis. however, a precise mechanism in suc ... | 1992 | 1336081 |
| hepatitis b virus vaccine in the gambia, west africa: synergy between public health research and practice. | hepatitis b virus infection is endemic in west africa, and as a result, this region has a high rate of hepatoma. the recent development of an effective, safe vaccine against hepatitis b virus that provides durable immunity may now make feasible the prevention of persistent hepatitis b virus infection and thus the prevention of hepatoma. a comprehensive primary health care program in which over 70% of children in the gambia, west africa, receive all their childhood immunizations afforded a unique ... | 1992 | 1336128 |
| risk assessment for aflatoxin b1: a modeling approach. | the data generated by yeh et al. on hepatitis b virus, aflatoxin, and primary hepatocellular carcinoma (phc) in southern guangxi, china was used to evaluate the cancer potency of aflatoxin. we examined model fits to these data to explore whether hepatitis b virus (hbv) and aflatoxin intake act together to affect phc rates in an additive, multiplicative, or interactive fashion, using relative and excess risk model forms. purely additive models fit the data poorly. fitted models were checked for p ... | 1992 | 1336206 |
| [association of liver cirrhosis, hepatitis b virus infection and hepatocellular carcinoma--immunohistochemical study]. | in order to assess frequency and importance of the presence of alpha fetoprotein (afp) and alachy as well as hepatitis b virus (hbv) infection antigens in the tissue of hepatocellular carcinoma (hcc) and in the liver tissue outside a tumour, there have been performed retrospective light-microscopic and immunohistochemical examinations of the liver tissue in 65 cases of hcc (39 autopsy and 26 biopsy cases). the finding of alachy in 100% of autopsy cases of hcc and 80.7% of biopsy cases of hcc com ... | 1992 | 1336256 |
| virus inactivation of fresh frozen plasma by a solvent detergent procedure: biological results. | in order to increase the safety of blood products, we have developed a procedure for the virus inactivation of fresh frozen plasma. several batches have been prepared and with the first 10 batches, each of them composed of 60 litres of plasma, we have determined a set of biological parameters. virus inactivation was realised using tnbp (1%) and octoxynol 9 (1%). after their elimination with castor oil using chromatography on insolubilized c18 resin, glycine was added and the ph of the plasma was ... | 1992 | 1336258 |
| the anti-hepatitis b virus activities, cytotoxicities, and anabolic profiles of the (-) and (+) enantiomers of cis-5-fluoro-1-[2-(hydroxymethyl)-1,3-oxathiolan-5-yl]cytosine. | the anti-hepatitis b (anti-hbv) activities of the (-) and (+) enantiomers of cis-5-fluoro-1-[2-(hydroxymethyl)-1,3-oxathiolan-5-yl]cytosine (2'-deoxy-3'-thia-5-fluorocytosine [ftc]) were studied by using an hbv-transfected cell line (hepg2 derivative 2.2.15, subclone p5a). the (-) isomer was found to be a potent inhibitor of viral replication, with an apparent 50% inhibitory concentration of 10 nm, while the (+) isomer was found to be considerably less active. both isomers showed minimal toxicit ... | 1992 | 1336341 |
| fallacies in the interpretation of paul-bunnel davidsohn differential test. | serological findings in five cases where paul-bunnel davidsohn (pbd) test results were misleading, are presented. three patients' with chronic renal disorder and positive pbd test had specific serology results, signifying cyto megalo virus infection. a fourth patient with hepatitis b virus infection also had positive pbd test. forssman type of antibody response was demonstrable in a boy with recent epstein-barr virus infection and high cyto megalo virus antibody titres. | 1992 | 1336481 |
| serum hepatitis c virus rna and hepatitis b virus dna in non-a, non-b post-transfusional and sporadic chronic hepatitis. | the sera of 36 french patients with post-transfusional and sporadic non-a, non-b chronic hepatitis were investigated, for hbv and hcv infections using a combination of serological and polymerase chain reaction (pcr) assays. anti-hcv was detected in 75% (27/36) of the patients by elisa1 and/or riba2 tests. hcv-rna sequences were found in 75% (27/36) of the sera by a single step pcr, using a set of primers located in the 5' non-coding region. altogether, 89% (32/36) of the patients were found posi ... | 1992 | 1336507 |
| the role of hepatitis b virus in the development of primary hepatocellular carcinoma: part i. | chronic infections with hepatitis b virus (hbv) of humans and animal hepadnavirus infections in their natural hosts are strongly associated with primary hepatocellular carcinoma (hcc). although viral integrations are found in cells of many hcc, no general viral-specific hepatocarcinogenic mechanism for hepadnaviruses has been identified. in approximately one half of hcc in woodchuck hepatitis virus (whv) infected woodchucks, viral integrations near the c-myc or n-myc genes have been reported whi ... | 1992 | 1336678 |
| pharmacokinetics and metabolism of racemic 2',3'-dideoxy-5-fluoro-3'-thiacytidine in rhesus monkeys. | 2',3'-dideoxy-5-fluoro-3'-thiacytidine (ftc) is a nucleoside analog that selectively inhibits human immunodeficiency and hepatitis b viruses in vitro. in this study, the preclinical pharmacokinetics of racemic ftc in rhesus monkeys following intravenous and oral administration were characterized. the terminal half-life of ftc was independent of the route of administration and averaged 1.34 +/- 0.18 h (mean +/- standard deviation). total clearance of ftc was moderate to high, averaging 1.49 +/- 0 ... | 1992 | 1336946 |
| [the role of hbv and hcv viruses in the pathogenesis of hepatic lesions in alcoholics]. | due to the high prevalence in the population of both viral hepatitis and alcoholism it is not uncommon to find patients with chronic hepatitis in whom both these stigmata are simultaneously present. in such cases, the risk of evolution to cirrhosis is thought to be greater. in this study was evaluated 104 alcoholic patients (50 of whom had hepatic biopsy), with or without antibodies (ab) to hepatitis c virus (hcv). in addition, we retrospectively examined 183 hepatic biopsies of alcoholic patien ... | 1992 | 1337153 |
| [changes in the gene expression of the hepatitis b virus in plc-prf-5 cells of human hepatocarcinoma during their acquisition of drug resistance]. | mutant clones of hepatocarcinoma cell line plc-prf-5 containing integrated hepatitis b virus genome were derived by multistep selection for resistance to some toxic agents. these clones were found to display various stages of cell differentiation according to growth rate, ability to synthesize alpha-fetoprotein, to grow in semisolid medium and to cloning in agar. in the majority of the clones with higher stages of differentiation the expression of hbsag gene was demonstrated to be increased as c ... | 1992 | 1337803 |
| [study on the expression of oncogenes in hepatocytes with hepatitis b virus infection]. | the expression of oncogene n-ras mrna and c-myc mrna in paraffin embedded liver tissues from patients with hepatitis b virus (hbv) infection had been studied by in situ hybridization with biotin labeled probes. the results showed that the detection rates of n-ras mrna and c-myc mrna were 37.5% (24/64) and 29.7% (19/64) respectively in the liver tissues of 64 hepatitis b patients, 44.4% (16/36) and 47.2% (17/36) respectively in the liver tissues of 36 hepatocellular carcinoma (hcc) patients with ... | 1992 | 1337870 |
| [immunohistochemical study on x antigen of hbv (hbxag) in primary hepatic carcinoma]. | specimens of 110 cases of primary hepatic carcinoma were obtained from the pathological laboratory of the first teaching hospital of the 4th military medical university, xi'an. p. r. china. sections from formalin-fixed and paraffin-embedded material were stained for hbxag by abc method and for hbsag and hbcag by pap method. among the 110 cases of primary hepatic carcinoma, 64 (58.2%) showed hbxag-positive reaction in tumor tissue, and 63 (78.8%) of 80 cases of noncancerous surrounding hepatic ti ... | 1992 | 1337887 |
| mathematical morphologic analysis of liver cirrhosis. correlation with etiology, clinical score and hepatocellular carcinoma. | remodeling of the cirrhotic liver was studied retrospectively by mathematical morphologic methods in 75 autopsy cases (40 alcoholic, 17 hepatitis b virus (hbv)-related and 18 cryptogenetic cirrhosis), including 28 hepatocellular carcinomas. the aim was to obtain objective measurements of cirrhotic patterns that could be correlated with liver function evaluated by the pugh-child score, establish the relationship among different morphogenetic features and evaluate the implications of an objective ... | 1992 | 1337967 |
| analysis of a cellular model to account for the natural history of infection by the hepatitis b virus and its role in the development of primary hepatocellular carcinoma. | infection with the hepatitis b virus (hbv) can have many different outcomes. transient infection may result in acute hepatitis or may remain subclinical. persistent infection may also be subclinical, or may involve chronic active hepatitis, and can finally lead to the development of primary hepatocellular carcinoma. a mathematical model is given to account for the many different outcomes of hbv pathogenesis. the model is based on the assumption that the liver contains two cell populations with d ... | 1992 | 1338219 |
| [a research for screening anti-hepatitis b virus drugs with the 2.2.15 cell line]. | in this paper, 43 drugs have been screened and evaluated for their ability of anti-hepatitis b virus by way of detecting in the cell culture medium the hbsag and hbeag secreted from the 2.2.15 cell line--a cell line derived from the hep-g2 transfected with cloned hbv dna. in addition, the mtt colorimetric assay is used in the process for monitoring the cytotoxic effects. the result showed that five drugs, including anti-hbv-i, anti-hbv-ii, radix astragali co., rhizoma curculiginis co. and 518bii ... | 1992 | 1338508 |
| anti-hcv antibodies and hepatocellular carcinoma. relationship in a medium-risk population. | by means of an accurate immunoenzymatic assay, the prevalence was studied of antibodies to hepatitis c virus (hcv) in three different populations: 74 patients affected with hepatocellular carcinoma (hcc) on preexisting cirrhosis, 82 patients with liver cirrhosis but with no apparent neoplasm, and 70 control subjects, hospitalized for various conditions, of internal medicine or geriatric interest. 70.2% of hcc patients exhibited anti-hbc antibodies, versus 47.5% of cirrhotic subjects with no tumo ... | 1992 | 1338660 |
| [hepatitis b virus infection in monocytes and their functional changes in patients with chronic hepatitis b]. | we detected the hbv dna in peripheral blood monocytes of 70 patients with chronic hepatitis b and 20 healthy controls by polymerase chain reaction (pcr) and southern transfer hybridization technique, the hla-dr expression on monocytic membrane and tumor necrosis factor-alpha (tnf alpha) content and interleukin 1 (il 1) activity in their monocytic culture supernatant were also measured respectively by indirect immunofluorescence technique, elisa, and thymocytic multiplication assay. hbv dna seque ... | 1992 | 1338700 |
| molecular dosimetry of aflatoxin-n7-guanine in human urine obtained in the gambia, west africa. | hepatocellular carcinoma is one of the major human cancers, causing at least 250,000 deaths each year. two of the major risk factors for this disease are aflatoxin exposure and hepatitis b virus. this study was undertaken to explore the relationship between dietary exposure to aflatoxins and the excretion of the major aflatoxin-dna adduct and other metabolites into the urine of chronically exposed people who were either hepatitis b virus surface antigen-positive or -negative. the diets of 20 ind ... | 1992 | 1339082 |
| the paul brousse liver transplant series 1989 to 1992: new trends in the last four years. | in the last four years, 551 liver transplantations have been performed at the paul brousse center, for a total of 840 liver transplantations performed from 1984 to 1992. several changes have been observed in the field of liver transplantation in the past years. the field of immunosuppression was marked mainly by the advent of fk506 as a preventive treatment of rejection and as a treatment of cortico-resistant rejection. results are still under analysis. from the surgical viewpoint, the main modi ... | 1992 | 1339127 |
| [current status in hepatitis virus research]. | seroconversion from eag to anti-e is frequently seen in the course of chronic hepatitis b infection. this phenomenon is closely related to mutation of the precore region; a g-to-a substitution of nucleotide position 1986 replaces tryptophan to translational stop codon. this mutant is responsible for the fulminant hepatitis or acute exacerbation of chronic hepatitis b. the hepatitis c virus shows a high rate of genome variations, especially at the envelope (e and ns1) region, where a neutralizing ... | 1992 | 1339164 |
| the seroepidemiology of hepatitis a and b in a japanese town. | sera collected from 1,118 healthy children and adults aged between four years and 90 years during the period 1989 to 1990, were tested for serological markers of hepatitis a virus (hav) [antibody to hav (anti-hav)] and hepatitis b virus (hbv) [hepatitis b surface antigen (hbsag) and antibody to hepatitis b surface antigen (anti-hbsab)]. the overall prevalence rates of anti-hav, hbsag, and anti-hbv were 20.2%, 0.36%, and 5.1%, respectively. no body was found to be positive for anti-hav below 30 y ... | 1992 | 1339221 |
| a second gene (quth) within the aspergillus nidulans-quinic-acid utilisation gene cluster encodes a protein with a putative zinc-cluster motif. | a sequence of 3299 nt, contiguous with the previously sequenced quinate permease-encoding (qutd) gene and encompassing the dehydroshikimate dehydratase-encoding (qutc) gene, has been determined. northern-blot analysis detected (i) a quinate-inducible mrna of the expected size for the qutc gene, and (ii) a quinate-inducible mrna of 1.45 kb divergently transcribed away from qutc towards qutd. computer-aided sequence analysis identified an orf of 1047 nt corresponding to the qutc gene encoding dehy ... | 1992 | 1339361 |
| [the hepatitis b virus and its markers: the evolution in 25 years]. | 1992 | 1340745 | |
| [hepatitis b virus markers in peripheral blood mononuclear cells]. | recent studies have shown tropism of the hepatitis b virus (hbv) by peripheral blood mononuclear cells (pbmc). the consequences of this phenomenon and their clinical use are not yet clear, however. seventy-nine patients were studied between march 1989 and october 1990. sixty-nine patients had chronic liver disease with histological evaluations, and 10 were vaccinated for hbv. the following markers were determined: serum: hbsag, hbeag, anti-hbe, antitotal-hbc, anti-hbs, anti-hcv, hbv-dna; lysated ... | 1992 | 1340746 |
| [hepatitis b (hbv) and c (hbc) virus infections in down's syndrome and in neuropsychiatric patients without down's syndrome]. | hepatitis b virus (hbv) markers are found with high frequency in immunocompromised individuals. in order to find out if this is also true for the hepatitis c virus (hcv), we have analyzed a group (g.1) of 46 patients (pts.) with down syndrome, situation known to be associated with immunodepression g. 1. we compared them with a g. of 310 mentally retarded pts. without down syndrome g. 2 and without evidence of immunological disfunction. all of them were studied for infection with hbv. all pts. in ... | 1992 | 1340826 |
| [sporadic non-a, non-b hepatitis in argentina. the responsibility of the hepatitis e virus?]. | sporadic non a, non b hepatitis non related to blood transfusions or parenteral exposure is present in india and various countries of southeast asia and africa. we present the experience in a northeastern region of argentina from january 1987 to september 1990. during this period twenty one patients where identified with acute hepatitis without serologic evidence of infection with hav, hbv, hcv, toxoplasmosis, infectious mononucleosis nor clinical evidence of herpes virus infection. clinic and b ... | 1992 | 1340827 |
| [pre s2: a marker for chronicity and/or liver damage in hepatitis b infection?]. | pre s2 was investigated using a monoclonal elisa in 100 samples from 52 patients with different forms of infection with hepatitis b virus. pre s2 was present in 18 of 19 patients with acute hepatitis. its persistence for more than 150 days after beginning of symptoms was associated to a chronic hepatitis state. it was also present in 11 patients with chronic hepatitis or cirrhosis, 15 asymptomatic hepatitis b carriers and 3 of 4 patients with hepatic carcinoma. pre s2 became negative in only 1 o ... | 1992 | 1340959 |
| [significance of investigating viral dna in serum from patients with hepatitis b]. | the dna-hbv was investigated in 109 serum samples from 75 patients with different forms of infection with the hb virus, using an ria hybridization technique (genostic-abott). dna-hbv was present in 12 of 18 cases of acute hepatitis, 1 of 3 patients with fulminant disease, 11 of 14 patients with chronic hepatitis and/or cirrhosis, 2 of 10 patients with hepatoma and 6 of 30 asymptomatic chronic carriers. presence of dna-hbv beyond 60 days in 7 patients with acute hepatitis established the chronic ... | 1992 | 1340975 |
| prospective randomized controlled trial of sequential treatment with corticoids and alpha-interferon versus treatment with interferon alone in patients with chronic active hepatitis b. | a randomized controlled trial was conducted to prospectively compare the efficacy of sequential treatment with corticoids and alpha-interferon versus treatment with interferon (ifn) alone in patients with chronic hepatitis b. | 1992 | 1341478 |
| in situ activation of b lymphocytes in hepatitis b virus-induced fulminant hepatitis. | 1992 | 1341484 | |
| hepatitis b virus and hepatitis delta virus. | 1992 | 1341643 | |
| association between leprosy and hepatitis b infection. a survey in goiânia, central brazil. | this investigation presents the results of hepatitis b virus screening among leprosy patients conducted in central brazil as a preliminary information for a hbv vaccination programme. the main objectives were to assess the seroprevalence of hbv serum markers among lepromatous patients and to analyse institutionalization as risk factor for hbv infection in this population. two groups of lepromatous patients were studied, 83 outpatients and 171 institutionalized ones. screening for hbv serum marke ... | 1992 | 1342105 |
| a case-control study on the association of hepatitis b virus infection and hepatocellular carcinoma in northeast brazil. | hepatitis b virus (hbv) serological markers were investigated in 40 incident cases of hepatocellular carcinoma (hcc) and in two age and sex matched control groups, comprising 40 patients with other cancers and 80 healthy individuals, resident in bahia, brazil. serologic tests were done by radioimmunoassay. the study observed high proportion of seropositivity to hbsag (42.5%) and of those presenting hbsag or antihbc (65.0%) among hcc cases, higher in men than women and in those aged 17 to 30 year ... | 1992 | 1342517 |
| prevalence of hepatitis b surface antigen and antibody among health care employees in negri sembilan, malaysia, 1989. | this study was based on a hepatitis b screening program conducted in one of the states in malaysia in 1989. the majority (84.6%) of the 2986 health employees were screened. one quarter (25%) was found to have serological markers for the hepatitis b virus (hbv); 2.1% had hepatitis b surface antigen (hbsag) and 22.8% had antibody to the hepatitis b surface antigen (anti-hbs). the occurrence of hbsag was higher in ethnic chinese (6.3%) compared to malays (1.8%) and indians (0.9%), even when analyze ... | 1992 | 1342800 |
| selective detection of human hepatitis b virus surface and core antigens in some peripheral blood mononuclear cell subsets by flow cytometry. | the presence of hbs and hbc antigens was investigated, by flow cytometry, on the surface of peripheral mononuclear cells (pbmc) from the following phenotype: cd3 (t lymphocytes), cd4 (t helper/inducer), cd8 (t cytotoxic/suppressor), cd19 (b lymphocytes) and cd56 (nk cells) among 8 patients suffering from chronic hepatitis b and 5 healthy hbv-negative subjects. this study demonstrated the presence of hbsag and hbcag on the lymphocyte surface for most of the patients. the mean percentage of labell ... | 1992 | 1343349 |
| [production and characterization of monoclonal antibodies directed against the hepatitis b surface antigen]. | hepatitis b virus surface antigen (hbsag) found in a commercial vaccine was use as immunogen and antigen for the production and selection of murine monoclonal antibodies against this viral antigen. antibody relative avidity was determined based on their capture capacity. competition studies, and the differential recognition pattern of vaccine preparations showed that high avidity igg1 antibodies were directed against two distinct antigenic regions. among them, 6f4 was most suitable for the detec ... | 1992 | 1343738 |
| portal hypertension in schistosomiasis: pathophysiology and treatment. | in heavily infected young patients, there is a "non-congestive" phase of the disease with splenomegaly which can improve after chemotherapy. a strong correlation between hepatosplenic form and worm burden in young patients has been repeatedly shown. the pattern of vascular intrahepatic lesions, seems to depend on two mechanisms: (a) egg embolization, with a partial blocking of the portal vasculature; (b) the appearance of small portal collaterals along the intrahepatic portal system. the role pl ... | 1992 | 1343892 |
| interrelationship between schistosomiasis and concomitant diseases. | the biological literature contains many examples of mutual influences between different species of parasites, especially with respect to concomitant helminth infections. several situations are known in which the association of infection by schistosoma mansoni with other pathogens in the same host results in a type of disease which differs from the simple summation of the individual effects of each infection. the present study concerns concomitant infections involving s. mansoni and enterobacteri ... | 1992 | 1343911 |
| risk of hepatitis b and human immunodeficiency virus transmission to a patient from an infected surgeon due to percutaneous injury during an invasive procedure: estimates based on a model. | the objective was to estimate the probability of sporadic hepatitis b virus (hbv) and human immunodeficiency virus (hiv) transmission to a patient from an infected surgeon due to percutaneous injury during an invasive procedure. risk was estimated based on a model involving three probabilities: a, the probability that the surgeon will sustain a percutaneous injury during an invasive procedure; b, the probability that the sharp object causing the injury and now contaminated with the surgeon's blo ... | 1992 | 1344665 |
| detection of hepatitis c virus rna sequences by polymerase chain reaction in fixed liver tissue. | currently, the most reliable method for the diagnosis of hepatitis c virus (hcv) infection is the detection of viral sequences by the reverse transcription double polymerase chain reaction (rt/pcr) in serum or liver samples. we demonstrate here that noncoding region sequences (nt) of hcv were amplifiable by rt/pcr in guanidinium extracts of formalin-fixed (for 6 to 48 h), paraffin-embedded liver sections of patients with chronic hepatitis c. in contrast, core and nonstructural region sequences o ... | 1992 | 1344813 |
| [the significance of lobular inflammation in chronic active hepatitis]. | authors have performed an anatomo-clinico-biological comparison of two groups of hepatitis with lobular inflammation: group i with a simple lobular inflammation, called lobular hepatitis, and group ii with a lobular inflammation associated with portal and periportal hepatitis, called chronic active hepatitis (cha) with lobular hepatitis. furthermore, a comparative study of these forms with a group of cha without lobular inflammation was performed. both forms of lobular inflammation appeared in y ... | 1992 | 1345056 |
| viral infection in the renal transplant recipient. | the viral infections with greatest impact on the renal transplant recipient are those due to cytomegalovirus, epstein-barr virus, and the two hepatitis viruses, hepatitis b and c. all of these are modulated by the administered immunosuppressive therapy, and all have both direct and indirect effects on the transplant patient. the direct effects are the infectious disease clinical syndromes that are produced (fever and malaise, pneumonia, hepatitis, and so forth). the indirect effects are several- ... | 1992 | 1345623 |
| post-transfusion fulminant hepatitis b after screening for hepatitis b virus core antibody. | 1992 | 1346219 | |
| induction of intercellular adhesion molecule-1 (cd54) on human hepatoma cell line hepg2: influence of cytokines and hepatitis b virus-dna transfection. | human hepatocyte expression of intercellular adhesion molecule-1 (icam-1) (cd54) was studied in vitro by exposing the well differentiated human hepatoblastoma cell line hepg2 to various cytokines. in addition, hepatitis b virus (hbv)-dna transfected hepg2 cells were also analysed. expression of icam-1 on hepg2 cells was then revealed with an immunohistochemical procedure. untreated hepg2 cells were unreactive, but showed strong cytoplasmic icam-1 immunoreactivity after treatment with interferon- ... | 1992 | 1346374 |
| lack of superiority of steroids plus plasma exchange to steroids alone in the treatment of polyarteritis nodosa and churg-strauss syndrome. a prospective, randomized trial in 78 patients. | to define the most effective treatment for polyarteritis nodosa (pan) and churg-strauss syndrome (css). | 1992 | 1346499 |
| pharmacokinetics of vidarabine in the treatment of polyarteritis nodosa. | the pharmacokinetics of vidarabine were studied in 8 patients with polyarteritis nodosa related to hepatitis b virus infection. the drug was administered by continuous infusion for three weeks at doses of 15 (1 week) and 7.5 (2 weeks) mg/kg per day, during which time 15 plasma exchanges were performed. plasma was assayed for vidarabine and its principal metabolite, hypoxanthine arabinoside by high pressure liquid chromatography. vidarabine was not detected in the plasma of any patients. hypoxant ... | 1992 | 1348236 |
| hepatitis b virus and apparent fulminant non-a, non-b hepatitis. | while there is evidence that hepatitis c virus (hcv) does not cause fulminant non-a, non-b hepatitis, the causal agent remains unknown. to evaluate the role of hepatitis b virus (hbv) in this disease, we used a two-step polymerase chain reaction (pcr) to amplify the surface and core regions of hbv dna in serum and liver samples taken prospectively from twenty-six patients (mean age 36 years, range 1 to 64) with acute hepatic failure undergoing liver transplantation. hbv dna was absent from the s ... | 1992 | 1348798 |