Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| recombinant salivary proteins of phlebotomus orientalis are suitable antigens to measure exposure of domestic animals to sand fly bites. | certain salivary proteins of phlebotomine sand flies injected into the host skin during blood-feeding are highly antigenic and elicit strong antibody-mediated immune responses in repeatedly-exposed hosts. these antibodies can be measured by enzyme-linked immuno sorbent assays (elisas) using salivary gland homogenates (sghs) as the source of antigens and serve as a markers for exposure to biting sand flies. large-scale screening for anti-sand fly saliva antibodies requires replacement of sgh with ... | 2016 | 26986566 |
| lutzomyia migonei is a permissive vector competent for leishmania infantum. | leishmania infantum is the most widespread etiological agent of visceral leishmaniasis (vl) in the world, with significant mortality rates in human cases. in latin america, this parasite is primarily transmitted by lutzomyia longipalpis, but the role of lutzomyia migonei as a potential vector for this protozoan has been discussed. laboratory and field investigations have contributed to this hypothesis; however, proof of the vector competence of l. migonei has not yet been provided. in this study ... | 2016 | 26988559 |
| molecular identification of lutzomyia migonei (diptera: psychodidae) as a potential vector for leishmania infantum (kinetoplastida: trypanosomatidae). | visceral leishmaniasis (vl) in brazil is caused by the protozoan leishmania infantum. this parasite is transmitted by the bite of a female sand fly. the most important sand fly species in vl transmission is lutzomyia longipalpis. in fortaleza, the capital of ceará state, brazil, the simultaneous occurrence of lutzomyia migonei and l. longipalpis was detected in localities where vl transmission is observed. the purpose of this study was to determine conclusively if l. migonei can be found natural ... | 2016 | 26995718 |
| digestion of yeasts and beta-1,3-glucanases in mosquito larvae: physiological and biochemical considerations. | aedes aegypti larvae ingest several kinds of microorganisms. in spite of studies regarding mosquito digestion, little is known about the nutritional utilization of ingested cells by larvae. we investigated the effects of using yeasts as the sole nutrient source for a. aegypti larvae. we also assessed the role of beta-1,3-glucanases in digestion of live yeast cells. beta-1,3-glucanases are enzymes which hydrolyze the cell wall beta-1,3-glucan polyssacharide. larvae were fed with cat food (control ... | 2016 | 27007411 |
| characterization of a digestive α-amylase in the midgut of pieris brassicae l. (lepidoptera: pieridae). | the current study deals with a digestive α-amylase in the larvae of pieris brassicae l. through purification, enzymatic characterization, gene expression, and in vivo effect of a specific inhibitor, acarbose. although α-amylase activity was the highest in the whole gut homogenate of larvae but compartmentalization of amylolytic activity showed an equal activity in posterior midgut (pm) and anterior midgut (am). a three step purification using ammonium sulfate, sepharyl g-100 and deae-cellulose f ... | 2016 | 27014094 |
| an evaluation of light-emitting diode (led) traps at capturing phlebotomine sand flies (diptera: psychodidae) in a livestock area in brazil. | a study to evaluate the use of light-emitting diodes (leds) as an attractant for phlebotomine sand flies at two animal pens in a livestock area in brazil was performed. light-suction traps were operated overnight with the following light sources: green, blue, and incandescent (control) lights. in total, 22 individual collections were made at each site and 44 with each trap type. in total, 2,542 specimens belonging to 14 phlebotomine species were collected. the most abundant species in the light ... | 2016 | 27026156 |
| seasonal and physiological variations of phlebotomus papatasi salivary gland antigens in central iran. | sand fly saliva helps parasite establishment and induce immune responses in vertebrate hosts. in the current study, we investigated the modulation of phlebotomus papatasi salivary gland antigen expression by seasonal and biological factors. | 2015 | 27047970 |
| visceral leishmaniasis in petrolina, state ofpernambuco, brazil, 2007-2013. | visceral leishmaniasis is a life-threatening disease of great public health relevance in brazil. the municipality of petrolina is an endemic area in the state of pernambuco, brazil. this study was designed to assess the recent expansion of vl in the municipality of petrolina, pernambuco. patients data were obtained from the brazilian national information system for notifiable diseases (sinan). a total of 111 records from 2007 to 2013 were investigated, of which 69 were residents in petrolina. th ... | 2016 | 27074323 |
| diagnostic doses and times for phlebotomus papatasi and lutzomyia longipalpis sand flies (diptera: psychodidae: phlebotominae) using the cdc bottle bioassay to assess insecticide resistance. | insecticide resistance to synthetic chemical insecticides is a worldwide concern in phlebotomine sand flies (diptera: psychodidae), the vectors of leishmania spp. parasites. the cdc bottle bioassay assesses resistance by testing populations against verified diagnostic doses and diagnostic times for an insecticide, but the assay has been used limitedly with sand flies. the objective of this study was to determine diagnostic doses and diagnostic times for laboratory lutzomyia longipalpis (lutz & n ... | 2016 | 27083417 |
| antissaliva antibodies of lutzomyia longipalpis in area of visceral leishmaniasis. | the aim of the present study was to assess the presence of antissaliva antibodies of lutzomyia longipalpis in human hosts living in area of visceral leishmaniasis, located in the center-west region of brazil. the presence of antissaliva antibodies of l. longipalpis exhibited a strong correlation with the protection and development of antibodies against leishmania sp. of the 492 children studied, elevated antissaliva antibodies of l. longipalpis were detected in 38.4% of the participants. there w ... | 2016 | 27093167 |
| the sandfly lutzomyia longipalpis ll5 embryonic cell line has active toll and imd pathways and shows immune responses to bacteria, yeast and leishmania. | lutzomyia longipalpis is the main vector of visceral leishmaniasis in latin america. sandfly immune responses are poorly understood. in previous work we showed that these vector insects respond to bacterial infections by modulating a defensin gene expression and activate the imd pathway in response to leishmania infection. aspects of innate immune pathways in insects (including mosquito vectors of human diseases) have been revealed by studying insect cell lines, and we have previously demonstrat ... | 2016 | 27098567 |
| leishmania infantum genetic diversity and lutzomyia longipalpis mitochondrial haplotypes in brazil. | leishmania infantum is the etiological agent of visceral leishmaniasis (vl) in the americas with domestic dogs being its major reservoir hosts. the main vl vector is the sandfly lutzomyia longipalpis, while other lutzomyia species may play a role in disease transmission. although the genetic structure of l. infantum populations has been widely evaluated, only a few studies have addressed this subject coupled to the genetic structure of the respective sandfly vectors. in this study, we analyzed t ... | 2016 | 27119085 |
| rhodnius prolixus and r. robustus (hemiptera: reduviidae) nymphs show different locomotor patterns on an automated recording system. | circadian rhythms of triatomines, vectors of the etiological agent trypanosoma cruzi responsible for chagas disease, have been extensively studied in adults of the two most epidemiologically relevant vector species, rhodnius prolixus and triatoma infestans. however, little attention has been dedicated to the activity patterns in earlier developmental stages, even though triatomine nymphs are equally capable of transmitting t. cruzi to humans. because circadian rhythms may differ even between clo ... | 2016 | 27121502 |
| the site of the bite: leishmania interaction with macrophages, neutrophils and the extracellular matrix in the dermis. | leishmania spp., the causative agents of leishmaniasis, are intracellular parasites, transmitted to humans via the bite of their sand fly vectors. once inoculated, the promastigotes are exposed to the dermis, which is composed of extracellular matrix (ecm), growth factors and its resident cells. promastigote forms are phagocytosed by macrophages recruited to the site of the sand fly bite, either directly or after interaction with neutrophils. since leishmania is an intracellular parasite, its in ... | 2016 | 27146515 |
| cutaneous leishmaniasis vaccination: a matter of quality. | there have been exhaustive efforts to develop an efficient vaccine against leishmaniasis. factors like host and parasite genetic characteristics, virulence, epidemiological scenarios, and, mainly, diverse immune responses triggered by leishmania species make the achievement of this aim a complex task. it is already clear that the induction of a th1, pro-inflammatory response, is important in the protection against leishmania infection. however, many questions must still be answered to fully unde ... | 2016 | 27148270 |
| occurrence of lutzomyia longipalpis andhuman and canine cases of visceral leishmaniasis and evaluation of their expansion in the northwest region of the state of são paulo, brazil. | introduction this paper aims to describe the dispersion of lutzomyia longipalpis and the autochthonous occurrence of visceral leishmaniasis (vl) in the northwest region of the state of são paulo between 2007 and 2013 and to analyze their expansion. methods information about the vector and associated cases was described using maps. the incidence, mortality, and lethality of human visceral leishmaniasis (hvl) were calculated. in municipalities in which more than one hvl case occurred, incidences w ... | 2016 | 27163563 |
| plant-derived compounds in treatment of leishmaniasis. | leishmaniasis is a neglected public health problem caused by the protozoan species belonging to the genus leishmania affecting mostly the poor populations of developing countries. the causative organism is transmitted by female sandflies. cutaneous, mucocutaneous, and visceral clinical manifestations are the most frequent forms of leishmaniasis. chemotherapy still relies on the use of pentavalent antimonials, amphotericin b, paromomycin, miltefosin and liposomal amphotericin b. however, the appl ... | 0 | 27175144 |
| xenodiagnosis on dogs with visceral leishmaniasis: canine and sand fly aspects related to the parasite transmission. | one of the main limitations for the effective control of canine leishmaniasis in endemic areas is the difficulty in identifying infectious dogs. the objective of this study was to determine factors, related to dogs and to parasite detection in sand flies, which are associated with the positive xenodiagnosis of leishmania infantum using the vector lutzomyia longipalpis. the xenodiagnosis was performed in 50 owned dogs residing in endemic areas, which were divided into three different groups: g1-2 ... | 2016 | 27198788 |
| abundance of lutzomyia longipalpis in urban households as risk factor of transmission of visceral leishmaniasis. | urban occurrence of human and canine visceral leishmaniasis (vl) is linked to households with characteristics conducive to the presence of sand flies. this study proposes an ad hoc classification of households according to the environmental characteristics of receptivity to phlebotominae and an entomological study to validate the proposal. here we describe the phlebotominae population found in intra- and peridomiciliary environments and analyse the spatiotemporal distribution of the vl vector lu ... | 0 | 27223866 |
| performance of conventional pcrs based on primers directed to nuclear and mitochondrial genes for the detection and identification of leishmania spp. | in visceral leishmaniasis, the detection of the agent is of paramount importance to identify reservoirs of infection. here, we evaluated the diagnostic attributes of pcrs based on primers directed to cytochrome-b (cytb), cytochrome-oxidase-subunit ii (coxii), cytochrome-c (cytc), and the minicircle-kdna. although pcrs directed to cytb, coxii, cytc were able to detect different species of leishmania, and the nucleotide sequence of their amplicons allowed the unequivocal differentiation of species ... | 2016 | 27253743 |
| comparative analysis of carbohydrate residues in the midgut of phlebotomines (diptera: psychodidae) from colony and field populations from amazon, brazil. | leishmaniasis are worldwide diseases that occur in 98 countries including brazil, transmitted by the bite of female phlebotomines during blood feeding. in brazil it is known that some species of sand flies as lutzomyia longipalpis sensun latum (vector of leishmania infantum chagasi), lutzomyia flaviscutellata (vector of leishmania (leishmania) amazonensis) and lutzomyia antunesi [suspected vector of leishmania (viannia) lindenbergi] are incriminated of transmitting the parasite leishmania for th ... | 2016 | 27264642 |
| taxonomy, diversity, temporal and geographical distribution of cutaneous leishmaniasis in colombia: a retrospective study. | leishmaniases are tropical zoonotic diseases, caused by kinetoplastid parasites from the genus leishmania. new world (nw) species are related to sylvatic cycles although urbanization processes have been reported in some south american countries such as colombia. currently, few studies show the relative distribution of leishmania species related to cutaneous leishmaniasis (cl) in south america due to the lack of accurate surveillance and public health systems. herein, we conducted a systematic es ... | 2016 | 27328969 |
| anthropophily of lutzomyia wellcomei (diptera: psychodidae) in an atlantic forest conservation unit in northeast brazil. | lutzomyia wellcomei (fraiha, shaw & lainson) (diptera: psychodidae) can act as an important vector of leishmania (viannia) braziliensis this study presents the results of collections carried out in a fragment of atlantic forest in a conservation unit of rio grande do norte state. collections occurred over 12 consecutive months using shannon and cdc traps. a total of 777 sand flies from eight species were collected: lutzomyia walkeri (newstead), lutzomyia evandroi (costa lima & antunes), lutzomyi ... | 2016 | 27330100 |
| differential expression profiles of the salivary proteins sp15 and sp44 from phlebotomus papatasi. | sand fly saliva has been shown to help parasite establishment and to induce immune responses in vertebrate hosts. in the current study, we investigated the pattern of expression of two phlebotomus papatasi salivary transcripts in specific physiological and seasonal conditions at a hyperendemic area of zoonotic cutaneous leishmaniasis (zcl) in iran. | 2016 | 27342811 |
| human and canine visceral leishmaniasis in an emerging focus in araçuaí, minas gerais: spatial distribution and socio-environmental factors. | this study aimed to analyse the spatial distribution of human (2007-2013) and canine (2013) visceral leishmaniasis (vl) in the city of araçuaí, minas gerais, brazil, and identify the socio-environmental factors related to their occurrence. the spatial distribution of human and canine cases was analysed by kernel density estimation (kde) and the k function. the kde values were analysed for correlation between human and canine lv and for normalised difference vegetation index (ndvi). socio-environ ... | 0 | 27384080 |
| canine antibody response to lutzomyia longipalpis saliva in endemic area of visceral leishmaniasis. | canine exposure to lutzomyia longipalpis bites and the potential of leishmania infantum transmissibility for the vector were evaluated. | 2017 | 27384835 |
| diffuse and disseminated cutaneous leishmaniasis: clinical cases experienced in ecuador and a brief review. | in ecuador, cutaneous leishmaniasis (cl) is prevalent countrywide, but only one case of diffuse-cl and two cases of disseminated-cl were experienced during our research activities more than 30 years from 1982 to date. these three patients suffered from multiple lesions distributed at a wide range of the body surface, revealing difficulty to clinically differentiate each other. | 2016 | 27398061 |
| molecular diversity between salivary proteins from new world and old world sand flies with emphasis on bichromomyia olmeca, the sand fly vector of leishmania mexicana in mesoamerica. | sand fly saliva has been shown to have proteins with potent biological activities, salivary proteins that can be used as biomarkers of vector exposure, and salivary proteins that are candidate vaccines against different forms of leishmaniasis. sand fly salivary gland transcriptomic approach has contributed significantly to the identification and characterization of many of these salivary proteins from important leishmania vectors; however, sand fly vectors in some regions of the world are still ... | 2016 | 27409591 |
| decentralized control of human visceral leishmaniasis in endemic urban areas of brazil: a literature review. | human migration and concomitant hiv infections are likely to bring about major changes in the epidemiology of some parasitic infections in brazil. human visceral leishmaniasis (hvl) control is particularly fraught with intricacies. it is against a backdrop of decentralized health care that the complex hvl control initiatives are brought to bear. this comprehensive review aims to explore the obstacles facing decentralized hvl control in urban endemic areas in brazil. | 2016 | 27433128 |
| dna barcode for the identification of the sand fly lutzomyia longipalpis plant feeding preferences in a tropical urban environment. | little is known about the feeding behavior of hematophagous insects that require plant sugar to complete their life cycles. we studied plant feeding of lutzomyia longipalpis sand flies, known vectors of leishmania infantum/chagasi parasites, in a brazilian city endemic with visceral leishmaniasis. the dna barcode technique was applied to identify plant food source of wild-caught l. longipalpis using specific primers for a locus from the chloroplast genome, ribulose diphosphate carboxylase. dna f ... | 2016 | 27435430 |
| experimental infection of phlebotomus perniciosus by bioluminescent leishmania infantum using murine model and artificial feeder. | leishmaniasis is a vector-borne disease that is transmitted by sandflies and caused by obligate intracellular protozoa of the genus leishmania. in the present study, we carried out a screening on the experimental infection of phlebotomus pernioucus by bioluminescent leishmania infantum using murine model and artificial feeder. we developed a real-time polymerase chain reaction (rt-pcr)-based method to determine individually the number of leishmania promastigotes fed by infected flies. among 1840 ... | 0 | 27439032 |
| comparison of optical microscopy and quantitative polymerase chain reaction for estimating parasitaemia in patients with kala-azar and modelling infectiousness to the vector lutzomyia longipalpis. | currently, the only method for identifying infective hosts with leishmania infantum to the vector lutzomyia longipalpis is xenodiagnosis. more recently, quantitative polymerase chain reaction (qpcr) has been used to model human reservoir competence by assuming that detection of parasite dna indicates the presence of viable parasites for infecting vectors. since this assumption has not been proven, this study aimed to verify this hypothesis. the concentration of amastigotes in the peripheral bloo ... | 0 | 27439033 |
| far beyond phagocytosis: phagocyte-derived extracellular traps act efficiently against protozoan parasites in vitro and in vivo. | professional mononuclear phagocytes such as polymorphonuclear neutrophils (pmn), monocytes, and macrophages are considered as the first line of defence against invasive pathogens. the formation of extracellular traps (ets) by activated mononuclear phagocytes is meanwhile well accepted as an effector mechanism of the early host innate immune response acting against microbial infections. recent investigations showed evidence that etosis is a widely spread effector mechanism in vertebrates and inve ... | 2016 | 27445437 |
| selective factors associated with the evolution of codon usage in natural populations of arboviruses. | arboviruses (arthropod borne viruses) have life cycles that include both vertebrate and invertebrate hosts with substantial differences in vector and host specificity between different viruses. most arboviruses utilize rna for their genetic material and are completely dependent on host trnas for their translation, suggesting that virus codon usage could be a target for selection. in the current study we analyzed the relative synonymous codon usage (rscu) patterns of 26 arboviruses together with ... | 2016 | 27455096 |
| purification and characterization of a novel kazal-type trypsin inhibitor from the leech of hirudinaria manillensis. | kazal-type serine proteinase inhibitors are found in a large number of living organisms and play crucial roles in various biological and physiological processes. although some kazal-type serine protease inhibitors have been identified in leeches, none has been reported from hirudinaria manillensis, which is a medically important leech. in this study, a novel kazal-type trypsin inhibitor was isolated from leech h. manillensis, purified and named as bdellin-hm based on the sequence similarity with ... | 2016 | 27455325 |
| characterization of a midgut mucin-like glycoconjugate of lutzomyia longipalpis with a potential role in leishmania attachment. | leishmania parasites are transmitted by phlebotomine sand flies and a crucial step in their life-cycle is the binding to the sand fly midgut. laboratory studies on sand fly competence to leishmania parasites suggest that the sand flies fall into two groups: several species are termed "specific/restricted" vectors that support the development of one leishmania species only, while the others belong to so-called "permissive" vectors susceptible to a wide range of leishmania species. in a previous s ... | 2016 | 27457627 |
| argentinian phlebotomine fauna, new records of phlebotominae (diptera: psychodidae) for the country and the province of chaco. | sand flies are insects of medical and veterinary importance, because some species are able to transmit several pathogens such as bartonella spp., phlebovirus spp., and protozoan parasites of the genus leishmania (ross). they are widely distributed in the americas, with recordings ranging from canada to argentina. approximately 500 phlebotominae species are known in the americas, of which it is considered that at least 56 are involved in the transmission of leishmaniasis (maroli et al. 2012). pre ... | 2016 | 27470814 |
| canine-based strategies for prevention and control of visceral leishmaniasis in brazil. | visceral leishmaniasis (vl) is a zoonosis found worldwide. its incidence has increased in brazil in recent years, representing a serious public and animal health problem. the strategies applied in brazil are questionable and are not sufficient to control the disease. thus, we have compared the efficacy of some of the currently available strategies focused on dogs to prevent and control zoonotic vl in endemic areas by optimizing a mathematical model. the simulations showed that the elimination of ... | 2016 | 27471852 |
| lipophosphoglycans from leishmania amazonensis strains display immunomodulatory properties via tlr4 and do not affect sand fly infection. | the immunomodulatory properties of lipophosphoglycans (lpg) from new world species of leishmania have been assessed in leishmania infantum and leishmania braziliensis, the causative agents of visceral and cutaneous leishmaniasis, respectively. this glycoconjugate is highly polymorphic among species with variation in sugars that branch off the conserved gal(β1,4)man(α1)-po4 backbone of repeat units. here, the immunomodulatory activity of lpgs from leishmania amazonensis, the causative agent of di ... | 2016 | 27508930 |
| saliva of rhipicephalus (boophilus) microplus (acari: ixodidae) inhibits classical and alternative complement pathways. | rhipicephalus (boophilus) microplus is the main ectoparasite affecting livestock worldwide. for a successful parasitism, ticks need to evade several immune responses of their hosts, including the activation of the complement system. in spite of the importance of r. microplus, previous work only identified one salivary molecule that blocks the complement system. the current study describes complement inhibitory activities induced by r. microplus salivary components and mechanisms elicited by puta ... | 2016 | 27515662 |
| visceral leishmaniasis in the state of sao paulo, brazil: spatial and space-time analysis. | to perform both space and space-time evaluations of visceral leishmaniasis in humans in the state of sao paulo, brazil. | 2016 | 27533364 |
| could phlebotomus mascittii play a role as a natural vector for leishmania infantum? new data. | the occurrence of phlebotomine sand flies in central europe was questioned until they were recorded for the first time in germany in 1999, and ten years later also in austria. the aim of this study was to investigate sand flies collected in austria for their carrier status of leishmania spp. | 2016 | 27542911 |
| impact of lbsapsal vaccine in canine immunological and parasitological features before and after leishmania chagasi-challenge. | dogs represent the most important domestic reservoir of l. chagasi (syn. l. infantum). a vaccine against canine visceral leishmaniasis (cvl) would be an important tool for decreasing the anxiety related to possible l. chagasi infection and for controlling human visceral leishmaniasis (vl). because the sand fly salivary proteins are potent immunogens obligatorily co-deposited during transmission of leishmania parasites, their inclusion in an anti-leishmania vaccine has been investigated in past d ... | 2016 | 27556586 |
| photoperiod differences in sand fly (diptera: psychodidae) species richness and abundance in caves in minas gerais state, brazil. | caves are unique habitats that are inhabited by a diverse and singular biota. among these inhabitants are sand flies, which are of great epidemiological interest in the neotropical region because they are vectors of leishmania the period of activity of these insects is usually crepuscular and nocturnal, but there are reports of diurnal activity of sand flies in caves. thus, the aim of this study was to evaluate the periodicity of daily activity of sand flies in cave environments in the municipal ... | 2016 | 27559123 |
| sandflies in an urban area of transmission of visceral leishmaniasis in midwest brazil. | the phlebotomine fauna of campo grande city, capital of mato grosso do sul state in brazil, an endemic area for visceral leishmaniasis, has been thoroughly investigated, but all the insect collections were undertaken with automatic light traps. the present study sought to investigate the fauna in this city using shannon and disney traps, having human beings and hamsters, respectively, as bait. both types of traps were installed in forest fragment and peridomiciliary areas in the period from 2007 ... | 2016 | 27593433 |
| crosstalk between purinergic receptors and lipid mediators in leishmaniasis. | leishmaniasis is a neglected tropical disease affecting millions of people around the world caused by organisms of the genus leishmania. parasite escape mechanisms of the immune system confer the possibility of resistance and dissemination of the disease. a group of molecules that has become a target for leishmania survival strategies are lipid mediators. among them, leukotriene b4 (ltb4) has been described as a pro-inflammatory molecule capable of activating cells of the immune system to combat ... | 2016 | 27595742 |
| circulating biomarkers of immune activation, oxidative stress and inflammation characterize severe canine visceral leishmaniasis. | clinical manifestations in canine visceral leishmaniasis (cvl) have not been clearly associated with immunological status or disease progression. we simultaneously assessed biomarkers of inflammation, immune activation, oxidative stress, and anti-sand fly saliva igg concentrations in dog sera with different clinical manifestations to characterize a biosignature associated with cvl severity. in a cross-sectional exploratory study, a random population of 70 dogs from an endemic area in brazil was ... | 2016 | 27595802 |
| differences in immune responses against leishmania induced by infection and by immunization with killed parasite antigen: implications for vaccine discovery. | the leishmaniases are a group of diseases caused by different species of the protozoan genus leishmania and transmitted by sand fly vectors. they are a major public health problem in almost all continents. there is no effective control of leishmaniasis and its geographical distribution is expanding in many countries. great effort has been made by many scientists to develop a vaccine against leishmaniasis, but, so far, there is still no effective vaccine against the disease. the only way to gener ... | 2016 | 27600664 |
| structural differences in gut bacteria communities in developmental stages of natural populations of lutzomyia evansi from colombia's caribbean coast. | lutzomyia evansi, a phlebotomine insect endemic to colombia's caribbean coast, is considered to be the main vector of visceral and cutaneous leishmaniasis in the region. although insects of this species can harbor pathogenic and non-pathogenic microorganisms in their intestinal microbiota, there is little information available about the diversity of gut bacteria present in lutzomyia evansi. in this study, conventional microbiological methods and molecular tools were used to assess the compositio ... | 2016 | 27618991 |
| dispersal of lutzomyia longipalpis and expansion of canine and human visceral leishmaniasis in são paulo state, brazil. | visceral leishmaniasis (vl), a neglected disease, is a serious public health problem that affects millions of people worldwide. the objectives of the study were to evaluate the sensitivity of lutzomyia longipalpis and canine vl (cvl) autochthony early detection and describe the spatial and temporal dispersal of vector and expansion of vl in a brazilian state. we obtained data on the leishmaniasis vector and vl cases in são paulo state (sp), brazil, from the division of endemic disease control an ... | 2016 | 27640322 |
| two in one: cryptic species discovered in biological control agent populations using molecular data and crossbreeding experiments. | there are many examples of cryptic species that have been identified through dna-barcoding or other genetic techniques. there are, however, very few confirmations of cryptic species being reproductively isolated. this study presents one of the few cases of cryptic species that has been confirmed to be reproductively isolated and therefore true species according to the biological species concept. the cryptic species are of special interest because they were discovered within biological control ag ... | 2016 | 27648231 |
| proceedings of the ix international symposium on phlebotomine sandflies (isops ix), reims, france, june 28th-july 1st, 2016. | 2016 | 27677701 | |
| nycthemeral rhythm of phlebotominae (diptera: psychodidae) in a craggy region, transitioning between the wetland and the plateau, brazil. | recording the nycthemeral rhythm of sand flies allows the evaluation of the daily activity in different ecotypes, the period of greatest activity, and their degree of anthropophily. we investigated the fauna and the rhythm of sand fly activity in an ecotourism region in mato grosso do sul (ms) state, brazil. sand flies were captured monthly, using a shannon trap for 24 h periods between july 2012 and june 2014. we collected 1,815 sand flies, in which lutzomyia whitmani (=nyssomyia whitmani, sens ... | 2016 | 27688269 |
| an increase of larval rearing temperature does not affect the susceptibility of phlebotomus sergenti to leishmania tropica but effectively eliminates the gregarine psychodiella sergenti. | in mosquitoes, it has previously been shown that rearing conditions of immature stages have an effect on the vector competence of adults. here, we studied the impact of different larval rearing temperatures (27 °c versus 32 °c) on the sand fly phlebotomus sergenti parrot, 1917 and its susceptibility to two parasites: leishmania tropica wright, 1903, a dixenous trypanosomatid transmissible from sand flies to humans, and psychodiella sergenti lantova, volf & votypka, 2010, a monoxenous sand fly gr ... | 2016 | 27756429 |
| molecular phylogeny of the megadiverse insect infraorder bibionomorpha sensu lato (diptera). | the phylogeny of the insect infraorder bibionomorpha (diptera) is reconstructed based on the combined analysis of three nuclear (18s, 28s, cad) and three mitochondrial (12s, 16s, coi) gene markers. all the analyses strongly support the monophyly of bibionomorpha in both the narrow (sensu stricto) and the broader (sensu lato) concepts. the major lineages of bibionomorpha sensu lato (sciaroidea, bibionoidea, anisopodoidea, and scatopsoidea) and most of the included families are supported as monoph ... | 2016 | 27781163 |
| environmental niche modelling of phlebotomine sand flies and cutaneous leishmaniasis identifies lutzomyia intermedia as the main vector species in southeastern brazil. | cutaneous leishmaniasis (cl) is caused by a protozoan of the genus leishmania and is transmitted by sand flies. the state of espírito santo (es), an endemic area in southeast brazil, has shown a considerably high prevalence in recent decades. environmental niche modelling (enm) is a useful tool for predicting potential disease risk. in this study, enm was applied to sand fly species and cl cases in es to identify the principal vector and risk areas of the disease. sand flies were collected in 46 ... | 2016 | 27783641 |
| monthly distribution of phlebotomine sand flies, and biotic and abiotic factors related to their abundance, in an urban area to which visceral leishmaniasis is endemic in corumbá, brazil. | the monthly distribution and abundance of sand flies are influenced by both biotic and abiotic factors. the present study aimed to evaluate the seasonal distribution of sand flies and the relation between their abundance and environmental parameters, including vegetation and climate. this study was conducted over a 2-year period (april 2012 to march 2014). monthly distribution was evaluated through the weekly deployment of cdc light traps in the peridomicile area of 5 residences in an urban area ... | 2016 | 27783667 |
| field evaluation of a new strategy to control lutzomyia longipalpis, based on simultaneous application of an adulticide-larvicide mixture. | leishmania infantum (syn. chagasi) is the etiologic agent of visceral leishmaniasis in argentina, and the phlebotomine fly lutzomyia longipalpis is its main vector. the objective of this study was to evaluate the effectiveness of dragon max(®), an emulsifiable concentrate formulation containing the pyrethroid permethrin and the larvicide pyriproxyfen, for lu. longipalpis control under field conditions. the work was conducted in the city of posadas (misiones province, argentina). comparisons were ... | 2016 | 27802395 |
| parasite load in the blood and skin of dogs naturally infected by leishmania infantum is correlated with their capacity to infect sand fly vectors. | the sand fly lutzomyia longipalpis is primarily responsible for the transmission of visceral leishmaniasis (vl) in the new world, and dogs are considered to be the main urban reservoir of this disease. in order to improve the efficacy of control measures, it is essential to assess the transmission capacity of leishmania infantum to the sand fly vector by naturally infected dogs. the present study investigated the existence of correlations between canine clinical presentation and the intensity of ... | 2016 | 27809965 |
| the diversity of yellow-related proteins in sand flies (diptera: psychodidae). | yellow-related proteins (yrps) present in sand fly saliva act as affinity binders of bioamines, and help the fly to complete a bloodmeal by scavenging the physiological signals of damaged cells. they are also the main antigens in sand fly saliva and their recombinant form is used as a marker of host exposure to sand flies. moreover, several salivary proteins and plasmids coding these proteins induce strong immune response in hosts bitten by sand flies and are being used to design protecting vacc ... | 2016 | 27812196 |
| presence of lutzomyia longipalpis (diptera: psychodidae) in the parque estadual da serra da tiririca, state of rio de janeiro, southeastern brazil. | the sand fly, lutzomyia longipalpis, is the main vector of leishmania infantum in the americas, primarily occurring in areas of apparent anthropomorphic modifications in several regions of brazil. | 2016 | 27812657 |
| insecticide resistance in phlebotomine sandflies in southeast asia with emphasis on the indian subcontinent. | visceral leishmaniasis, commonly known as kala-azar in india, is a global public health problem. in southeast asia, bangladesh, bhutan, india, nepal, sri lanka and thailand are endemic for visceral leishmaniasis. the role of sandflies as the vector of kala-azar was first confirmed in 1942 in india. insecticide resistance in phlebotomus argentipes annandale and brunetti, the vector of kala-azar in the indian subcontinent, was first reported in 1987 in bihar, india. this article provides a scoping ... | 2016 | 27817749 |
| diversity of bacteriome associated with phlebotomus chinensis (diptera: psychodidae) sand flies in two wild populations from china. | sand fly phlebotomus chinensis is a primary vector of transmission of visceral leishmaniasis in china. the sand flies have adapted to various ecological niches in distinct ecosystems. characterization of the microbial structure and function will greatly facilitate the understanding of the sand fly ecology, which would provide critical information for developing intervention strategy for sand fly control. in this study we compared the bacterial composition between two populations of ph. chinensis ... | 2016 | 27819272 |
| leishmania infantum infection in dogs from the southern region of minas gerais state, brazil. | visceral leishmaniasis is a systemic and chronic disease and dogs are the main reservoir of the etiologic agent, leishmania infantum (syn l. chagasi). a serological and molecular investigation of canine visceral leishmaniasis (cvl) was performed in the municipality of alfenas, located in the southern region of minas gerais, where the disease is not endemic. samples from 87 dogs were submitted to serological tests including the dual path platform (dpp (r) ) cvl bio-manguinhos rapid test, an in-ho ... | 2016 | 27828616 |
| prevalence of american trypanosomiasis and leishmaniases in domestic dogs in a rural area of the municipality of são joão do piauí, piauí state, brazil. | chagas disease and the leishmaniases are endemic zoonoses of great importance to public health in the state of piauí, brazil. the domestic dog (canis familiaris) is a major reservoir, host of trypanosoma cruzi and leishmania spp. in both urban and rural areas, playing an important role in the transmission of these parasites. the present study evaluated the prevalence of both infectious diseases in dogs of a rural area in the municipality of são joão do piauí, piauí state. one hundred twenty-nine ... | 2016 | 27828620 |
| the lutzomyia longipalpis complex: a brief natural history of aggregation-sex pheromone communication. | in this paper we review the natural history of pheromone communication and the current diversity of aggregation-sex pheromones in the sand fly lutzomyia longipalpis. this species complex is the main vector of leishmania infantum, the agent of visceral leishmaniasis in the americas. the identification of variation in pheromone chemotypes combined with molecular and sound analyses have all contributed to our understanding of the extent of divergence among cryptic members of this complex. the impor ... | 2016 | 27842601 |
| evolutionarily conserved odorant receptor function questions ecological context of octenol role in mosquitoes. | olfaction is a key insect adaptation to a wide range of habitats. in the last thirty years, the detection of octenol by blood-feeding insects has been primarily understood in the context of animal host-seeking. the recent discovery of a conserved octenol receptor gene in the strictly nectar-feeding elephant mosquito toxorhynchites amboinensis (taor8) suggests a different biological role. here, we show that taor8 is a functional ortholog of its counterparts in blood-feeding mosquitoes displaying ... | 2016 | 27849027 |
| screening of fungi for biological control of a triatomine vector of chagas disease: temperature and trypanosome infection as factors. | entomopathogenic fungi have been investigated as an alternative tool for controlling various insects, including triatomine vectors of the protozoan trypanosoma cruzi, the etiological agent of chagas disease. here we tested the pathogenicity and virulence of ten isolates of the fungi metarhizium spp. and beauveria bassiana against rhodnius prolixus and found all of the isolates to be virulent. we used two isolates (urpe-11 metarhizium anisopliae and ent-1 beauveria bassiana) for further screening ... | 2016 | 27855217 |
| essential oil composition, antimicrobial and pharmacological activities of lippia sidoides cham. (verbenaceae) from são gonçalo do abaeté, minas gerais, brazil. | lippia sidoides (verbenaceae) is used in brazilian folk medicine as an antiseptic, and it is usually applied topically on skin, mucous membranes, mouth, and throat, or used for vaginal washings. | 0 | 27867267 |
| proteomic profile of the bradysia odoriphaga in response to the microbial secondary metabolite benzothiazole. | benzothiazole, a microbial secondary metabolite, has been demonstrated to possess fumigant activity against sclerotinia sclerotiorum, ditylenchus destructor and bradysia odoriphaga. however, to facilitate the development of novel microbial pesticides, the mode of action of benzothiazole needs to be elucidated. here, we employed itraq-based quantitative proteomics analysis to investigate the effects of benzothiazole on the proteomic expression of b. odoriphaga. in response to benzothiazole, 92 of ... | 2016 | 27883048 |
| association between leishmania infantum dna in the hair of dogs and their infectiousness to lutzomyia longipalpis. | diagnosis of infection with leishmania infantum by dna detection in the hair has been recently demonstrated in dogs and wild animals. our objective was to investigate if polymerase chain reaction (pcr) in hair might be used to identify infectious dogs. thus, we assessed the infectiousness to lutzomyia longipalpis by xenodiagnosis in comparison with the detection of l. infantum dna by pcr in the hair, and with serology for anti-leishmania igg by elisa in 15 positive dogs for l. infantum infection ... | 2016 | 27890081 |
| prevalence of cutaneous leishmaniasis in districts of high and low endemicity in mali. | historically the western sahelian dry regions of mali are known to be highly endemic for cutaneous leishmaniasis (cl) caused by leishmania major, while cases are rarely reported from the southern savanna forest of the country. here, we report baseline prevalence of cl infection in 3 ecologically distinct districts of mali (dry sahelian, north savanna and southern savanna forest areas). we screened 195 to 250 subjects from 50 to 60 randomly selected households in each of the 6 villages (four from ... | 2016 | 27898671 |
| effect of the saliva from different triatomine species on the biology and immunity of tlr-4 ligand and trypanosoma cruzi-stimulated dendritic cells. | triatomines are blood-sucking vectors of trypanosoma cruzi, the causative agent of chagas disease. during feeding, triatomines surpass the skin host response through biomolecules present in their saliva. dendritic cells (dcs) play a crucial role in the induction of the protection to aggressive agents, including blood-sucking arthropods. here, we evaluated if salivary components of triatomines from different genera evade the host immunity by modulating the biology and the function of lps- or t. c ... | 2016 | 27938380 |
| sand flies (diptera: psychodidae) in an endemic area of leishmaniasis in aquidauana municipality, pantanal of mato grosso do sul , brazil. | the aquidauana municipality is considered an endemic area of leishmaniasis and an important tourist site in mato grosso do sul state. the aim of this study was to investigate the sand fly fauna in the city of aquidauana. captures were carried out twice a month, from april 2012 to march 2014 with automatic light traps and active aspiration, in the peridomicile and domicile of six residences. a total of 9,338 specimens were collected, 3,179 and 6,159 using light traps and active aspiration, respec ... | 2016 | 27982353 |
| sandflies (diptera: psychodidae) in an urban area of northeastern brazil. | the sandfly fauna is well studied globally. in brazil, sandfly fauna is very diverse in the northeast region, especially in states such as maranhão, ceará, and bahia. however, in the state of pernambuco, the distribution of these insects is still not well known. therefore, the objective of this study was to identify the different species that constitute the sandfly fauna in an urban area in the northeast region of brazil, where an outbreak of visceral leishmaniasis (vl) was recently reported. | 2017 | 28001216 |
| increasing potential risk for american visceral leishmaniasis in amapá, brazil. | the present note discusses some evidence on the increasing potential risk for american visceral leishmaniasis (avl) transmission in the northern brazilian state of amapá, the guianan-amazon biome. | 2017 | 28001227 |
| leg loss in lutzomyia longipalpis (diptera: psychodidae) due to pyrethroid exposure: toxic effect or defense by autotomy? | phlebotomine sandflies lose their legs after exposure to pyrethroids. in some insects leg loss helps to defend them from intoxication and predation, a phenomenon known as autotomy. a field observation has shown that sandflies that have lost some legs are still able to blood-feed. the aims of the study were to determine whether leg loss in sandflies, after exposure to deltamethrin, is due to autotomy and to establish the effect of the leg loss on blood-feeding. | 2017 | 28035108 |
| insect vectors of disease: untapped reservoirs for new antimicrobials? | with the increase in antibiotic resistance among infectious diseases, the need for new strategies for identifying compounds with inhibitory effects is dire. traditional methods of genome sequencing and systematic characterization of potential antimicrobial gene clusters, although effective, are unfortunately not yielding results at a speed consistent with the rise in antimicrobial resistance. one approach could be to use a more targeted approach to antimicrobial compound discovery. insect vector ... | 2016 | 28066398 |
| the search for novel insecticide targets in the post-genomics era, with a specific focus on g-protein coupled receptors. | insects are considered pests globally, implicated in the destruction of agricultural fields and transmission of pathogens that cause deadly human diseases, such as dengue, zika and malaria. the diversity of the insecticide arsenal has remained stagnant for decades, but the recent rise of insecticide resistance fueled the discovery of novel modes of action, and the power of genomics has reinvigorated this search. this review discusses the importance of comparative and functional insect genomics i ... | 2017 | 28076467 |
| first description of leishmania (viannia) infection in evandromyia saulensis, pressatia sp. and trichophoromyia auraensis (psychodidae: phlebotominae) in a transmission area of cutaneous leishmaniasis in acre state, amazon basin, brazil. | studies on the sandfly fauna to evaluate natural infection indexes are still limited in the brazilian amazon, a region with an increasing incidence of cutaneous leishmaniasis. here, by using a multiplex polymerase chain reaction directed to leishmania kdna and hybridisation, we were able to identify l. (viannia) subgenus in 12 out of 173 sandflies captured in the municipality of rio branco, acre state, revealing a positivity of 6.94%. by sequencing the leishmania 234 bp-hsp70 amplified products ... | 2017 | 28076470 |
| photoperiod differences in sand fly (diptera: psychodidae) species richness and abundance in caves in minas gerais state, brazil. | caves are unique habitats that are inhabited by a diverse and singular biota. among these inhabitants are sand flies, which are of great epidemiological interest in the neotropical region because they are vectors of leishmania the period of activity of these insects is usually crepuscular and nocturnal, but there are reports of diurnal activity of sand flies in caves. thus, the aim of this study was to evaluate the periodicity of daily activity of sand flies in cave environments in the municipal ... | 2017 | 28082636 |
| nycthemeral rhythm of phlebotominae (diptera: psychodidae) in a craggy region, transitioning between the wetland and the plateau, brazil. | recording the nycthemeral rhythm of sand flies allows the evaluation of the daily activity in different ecotypes, the period of greatest activity, and their degree of anthropophily. we investigated the fauna and the rhythm of sand fly activity in an ecotourism region in mato grosso do sul (ms) state, brazil. sand flies were captured monthly, using a shannon trap for 24 h periods between july 2012 and june 2014. we collected 1,815 sand flies, in which lutzomyia whitmani (=nyssomyia whitmani, sens ... | 2017 | 28082638 |
| comparative genomics of canine-isolated leishmania (leishmania) amazonensis from an endemic focus of visceral leishmaniasis in governador valadares, southeastern brazil. | leishmaniasis is a highly diverse group of diseases caused by kinetoplastid of the genus leishmania. these parasites are taxonomically diverse, with human pathogenic species separated into two subgenera according to their development site inside the alimentary tract of the sand fly insect vector. the disease encompasses a variable spectrum of clinical manifestations with tegumentary or visceral symptoms. among the causative species in brazil, leishmania (leishmania) amazonensis is an important e ... | 2017 | 28091623 |
| evidence of neofunctionalization after the duplication of the highly conserved polycomb group gene caf1-55 in the obscura group of drosophila. | drosophila caf1-55 protein is a subunit of the polycomb repressive complex prc2 and other protein complexes. it is a multifunctional and evolutionarily conserved protein that participates in nucleosome assembly and remodelling, as well as in the epigenetic regulation of a large set of target genes. here, we describe and analyze the duplication of caf1-55 in the obscura group of drosophila. paralogs exhibited a strong asymmetry in evolutionary rates, which suggests that they have evolved accordin ... | 2017 | 28094282 |
| the gut microbiome of the vector lutzomyia longipalpis is essential for survival of leishmania infantum. | the vector-borne disease leishmaniasis, caused by leishmania species protozoa, is transmitted to humans by phlebotomine sand flies. development of leishmania to infective metacyclic promastigotes in the insect gut, a process termed metacyclogenesis, is an essential prerequisite for transmission. based on the hypothesis that vector gut microbiota influence the development of virulent parasites, we sequenced midgut microbiomes in the sand fly lutzomyia longipalpis with or without leishmania infant ... | 2017 | 28096483 |
| canine visceral leishmaniasis in the metropolitan area of são paulo: pintomyia fischeri as potential vector of leishmania infantum. | american visceral leishmaniasis is a zoonosis caused by leishmania infantum and transmitted mainly by lutzomyia longipalpis. however, canine cases have been reported in the absence of this species in the greater são paulo region, where pintomyia fischeri and migonemyia migonei are the predominant species. this raises the suspicion that they could be acting as vectors. therefore, this study sought to investigate specific vector capacity parameters of these species and to compare them with those o ... | 2017 | 28134092 |
| risk analysis and prediction of visceral leishmaniasis dispersion in são paulo state, brazil. | visceral leishmaniasis (vl) is an important neglected disease caused by a protozoan parasite, and represents a serious public health problem in many parts of the world. it is zoonotic in europe and latin america, where infected dogs constitute the main domestic reservoir for the parasite and play a key role in vl transmission to humans. in brazil this disease is caused by the protozoan leishmania infantum chagasi, and is transmitted by the sand fly lutzomyia longipalpis. despite programs aimed a ... | 2017 | 28166251 |
| maxadilan-simile expression in nyssomyia neivai, a sandfly vector in an endemic region of brazil, and its immunogenicity in patients with american tegumentary leishmaniasis. | maxadilan (max) is a salivary component in the sandfly lutzomyia longipalpis (lutz & neiva 1912), a vector of visceral leishmaniasis. max has a powerful vasodilatory effect and is a candidate vaccine that has been tested in experimental leishmaniasis. nyssomyia neivai (pinto 1926) is a vector of the pathogen responsible for american tegumentary leishmaniasis (atl) in brazil. | 2017 | 28177045 |
| autochthonous outbreak and expansion of canine visceral leishmaniasis, uruguay. | we report an outbreak of canine visceral leishmaniasis in uruguay. blood specimens from 11/45 dogs tested positive for leishmania spp. specimens of lutzomyia longipalpis sand flies were captured; typing revealed leishmania infantum. our findings document an expansion of visceral leishmaniasis to southern south america and risk for vectorborne transmission to humans. | 2017 | 28221113 |
| the current status of the lutzomyia longipalpis (diptera: psychodidae: phlebotominae) species complex. | lutzomyia longipalpis s.l. is a complex of sibling species and is the principal vector of american visceral leishmaniasis. the present review summarises the diversity of efforts that have been undertaken to elucidate the number of unnamed species in this species complex and the phylogenetic relationships among them. a wide variety of evidence, including chemical, behavioral and molecular traits, suggests very recent speciation events and complex population structure in this group. although signi ... | 2017 | 28225906 |
| structure of salo, a leishmaniasis vaccine candidate from the sand fly lutzomyia longipalpis. | immunity to the sand fly salivary protein salo (salivary anticomplement of lutzomyia longipalpis) protected hamsters against leishmania infantum and l. braziliensis infection and, more recently, a vaccine combination of a genetically modified leishmania with salo conferred strong protection against l. donovani infection. because of the importance of salo as a potential component of a leishmaniasis vaccine, a plan to produce this recombinant protein for future scale manufacturing as well as knowl ... | 2017 | 28278244 |
| seasonality of sand flies (diptera: psychodidae) and leishmania dna detection in vector species in an area with endemic visceral leishmaniasis. | leishmaniases are a serious health problem in southeast brazil, including the city of belo horizonte (bh), minas gerais state (mg), where there are high rates of incidence and mortality due to visceral leishmaniases. bh is divided into nine sanitary districts (sd) of which one, the venda nova sd, was selected for this study because it has high rates of positivity for canine leishmaniasis and high incidence of human leishmaniasis. | 2017 | 28327794 |
| increased transmissibility of leishmania donovani from the mammalian host to vector sand flies after multiple exposures to sand fly bites. | patients with active visceral leishmaniasis are important reservoirs in the anthroponotic transmission cycle of leishmania donovani. the role of the blood or skin as a source of infection to sand flies remains unclear, and the possible effect of multiple exposures to fly bites on transmissibility has not been addressed. | 2017 | 28329329 |
| bloodmeal identification in field-collected sand flies from casa branca, brazil, using the cytochrome b pcr method. | pcr-based identification of vertebrate host bloodmeals has been performed on several vectors species with success. in the present study, we used a previously published pcr protocol followed by dna sequencing based on primers designed from multiple alignments of the mitochondrial cytochrome b gene used to identify avian and mammalian hosts of various hematophagous vectors. the amplification of a fragment encoding a 359 bp sequence of the cyt b gene yielded recognized amplification products in 192 ... | 2017 | 28399200 |
| first record of lutzomyia (lutzomyia) longipalpis (diptera: psychodidae: phlebotominae) on the trinational frontier (brazil-peru-bolivia) of south-western amazonia. | in south america, the main sand fly species involved in the transmission of leishmania infantum chagasi (cunha & chagas, 1937), etiological agent of the visceral leishmaniasis (vl), is lutzomyia longipalpis (lutz & neiva, 1912). the species has been recorded in colombia, venezuela, bolivia, argentina, paraguay, uruguay, and brazil, where it is recorded in 24 of the 27 brazilian states, except acre, amazonas, and santa catarina. collections were carried out for one year (april 2013 to march 2014) ... | 2017 | 28453779 |
| occurrence and probability maps of lutzomyia longipalpis and lutzomyia cruzi (diptera: psychodidae: phlebotominae) in brazil. | leishmaniases are serious diseases caused by trypanosomatid protozoans of the genus leishmania transmitted by the bite of phlebotomine sand flies. we analyzed records pertaining to lutzomyia longipalpis (lutz and neiva, 1912) and lutzomyia cruzi (mangabeira, 1938) in brazil from the following sources: the collection of phlebotomine sand flies of the centro de pesquisas rené rachou/fiocruz (fiocruz-colfleb), the "specieslink" (cria) database, from systematic surveys of scientific articles and gra ... | 2017 | 28472338 |
| in vitro inhibition of leishmania attachment to sandfly midguts and ll-5 cells by divalent metal chelators, anti-gp63 and phosphoglycans. | leishmania braziliensis and leishmania infantum are the causative agents of cutaneous and visceral leishmaniasis, respectively. several aspects of the vector-parasite interaction involving gp63 and phosphoglycans have been individually assayed in different studies. however, their role under the same experimental conditions was not studied yet. here, the roles of divalent metal chelators, anti-gp63 antibodies and purified type i phosphoglycans (pgs) were evaluated during in vitro parasite attachm ... | 2017 | 28472733 |
| leishmania infantum dna detected in phlebotomine species from puerto iguazú city, misiones province, argentina. | in puerto iguazú city, argentina, human and canine visceral leishmaniasis cases have been recorded since the year 2010, with leishmania infantum as the etiological agent and lutzomyia longipalpis as its main vector. in the present study, polymerase chain reaction and sequencing were used to detect l. infantum dna in 3.9% of the female sandflies captured in puerto iguazú city. this is the first report of l. infantum dna detection in micropygomyia quinquefer, and the second one in lu. longipalpis ... | 2017 | 28476601 |
| standardization of a continuous assay for glycosidases and its use for screening insect gut samples at individual and populational levels. | glycoside hydrolases (ghs) are enzymes able to recognize and cleave glycosidic bonds. insect ghs play decisive roles in digestion, in plant-herbivore, and host-pathogen interactions. gh activity is normally measured by the detection of a release from the substrate of products as sugars units, colored, or fluorescent groups. in most cases, the conditions for product release and detection differ, resulting in discontinuous assays. the current protocols result in using large amounts of reaction mix ... | 0 | 28553236 |
| ecological parameters of the (s)-9-methylgermacrene-b population of the lutzomyia longipalpis complex in a visceral leishmaniasis area in são paulo state, brazil. | visceral leishmaniasis (vl) is an important public health challenge in brazil because of the high number of human and canine cases reported annually. leishmania infantum is the etiological agent of vl and lutzomyia longipalpis is its main vector. however, evidence suggests that this taxon constitutes a species complex. in sao paulo state, there are two populations of lu. longipalpis, each secreting distinct pheromones, (s)-9-methylgermacrene-b and cembrene 1; both have been associated with diffe ... | 2017 | 28558760 |