Publications

effects of amiloride on na+ content and pinocytosis in entamoeba histolytica.	amiloride, a blocker of na+ leak and na+-h+ exchange in animal cells, caused cells of entamoeba histolytica to release na+ (up to 40% of their original na+ content within 90 min, at an amiloride concentration of 3 mm); k+ content was not affected. by comparing the unidirectional uptake of 22na+ with that of the fluid-phase marker 125i-labeled poly(vinylpyrrolidone) we established that the amiloride-induced na+ loss was due to inhibition of pinocytic na+ uptake rather than to blockage of an amilo ...	1986	2867784
molecular mechanisms of cytotoxicity mediated by entamoeba histolytica: characterization of a pore-forming protein (pfp).		1985	2868017
detection of amoebic antigen by enzyme linked immuno-sorbent assay (elisa).		1985	2868123
growth studies on axenic entamoeba histolytica and naegleria fowleri.		1985	2868550
antibodies to entamoeba histolytica in patients with rheumatoid arthritis.		1985	2868552
distinction between entamoeba gingivalis and entamoeba histolytica, revisited.		1986	2868598
cutaneous amebiasis of the face: a case report.	this paper describes a case of cutaneous amebiasis of the face in a 17-month-old girl. about four weeks prior to admission she had diarrhea with high suspicion of intestinal amebiasis. fifteen days later she presented with edema of the right lower eyelid and epiphora. there was a vesicle in the inner angle of the eye which spontaneously drained, causing loss of the underlying tissue. the ulcer developed rapidly, destroying periorbital tissue. both cytology and biopsy of the ulceration border sho ...	1986	2868670
differences in adhesion, phagocytosis and virulence of clones from entamoeba histolytica, strain hm1:imss.		1985	2869003
activation of complement by pathogenic and nonpathogenic entamoeba histolytica.	previous studies had demonstrated that strains of entamoeba histolytica isolated from patients with colitis or amebic liver abscess were resistant to complement-mediated killing, whereas strains from asymptomatic patients were readily lysed by non-immune serum. both serum-sensitive and serum-resistant strains of e. histolytica depleted complement rapidly as assessed by ch50, c3, and c7, and c5-9 hemolytic activities. activation of the alternative pathway was important in lysis of nonpathogenic s ...	1986	2869084
patients treated for amebic liver abscess develop cell-mediated immune responses effective in vitro against entamoeba histolytica.	we studied the afferent and efferent cell-mediated immune response in 15 patients treated for amebic liver abscess. patients had a lower t4 to t8 ratio (1.25 +/- 0.65) compared with age- and sex-matched controls (1.89 +/- 0.44, p less than 0.01) due to a decrease in t4-"helper" cells and an increase in t8-"suppressor" cells (p less than 0.01). the in vitro proliferative response of patient t lymphocytes to the plant mitogen concanavalin a (con a) was depressed; responses to phytohemagglutinin we ...	1986	2869086
isoenzyme analysis of entamoeba histolytica isolated from homosexual men.		1986	2869090
the major neutral proteinase of entamoeba histolytica.	fplc anion-exchange and chromatofocusing chromatography were used to purify the major neutral proteinase from secretions of axenically cultured entamoeba histolytica trophozoites. hm-1 strain trophozoites, which were more proteolytically active than the less virulent hk-9 strain, were used for purification of the enzyme. it is a thiol proteinase with a subunit mr of approximately 56,000, a neutral ph optimum, and a pi of 6. the importance of this enzyme in extraintestinal amoebiasis is suggested ...	1986	2869098
primates as a source of entamoeba histolytica, their zymodeme status and zoonotic potential.	a survey of 17 species of non-human captive primates in quebec, canada, revealed that 40% of the animals were infected with the protozoan entamoeba histolytica. isoenzyme analysis of lysates prepared from cultured amebic isolates showed them to belong to either a zymodeme iii or viii, and therefore, characteristic of non-pathogenic strains of e. histolytica from humans. these isolates were also of low virulence in gerbil ceca. serum samples from infected and uninfected primates had negative titr ...	1985	2869116
is entamoeba histolytica in homosexual men a pathogen?	entamoeba histolytica (eh) in homosexual men is generally considered to be pathogenic. to test this hypothesis, the generally accepted features of invasion (haematophagous trophozoites in faeces; high-titre serum antibody; moderate to severe acute inflammatory change; and presence of eh in the mucosa on rectal biopsy) and the zymodeme pattern of cultured trophozoites were assessed in twenty-three eh excretors and eleven control homosexual men. no trophozoites or antibody to eh were found in eith ...	1986	2869346
assessment of validity of counterimmunoelectrophoresis and elisa in the routine diagnosis of amoebiasis.	counterimmunoelectrophoresis (cie) was used to detect antibodies against entamoeba histolytica in 99 sera from amoebiasis suspected patients using antigens from the hk-9 and ht-12 strains of axenically cultivated e. histolytica. there was no significant difference of seropositive rates using antigens from these two strains suggesting the both strains were equally suitable for use in the routine cie test for the diagnosis of amoebiasis. in comparison with elisa, the cie test was a little less sen ...	1985	2869587
fulminant amebic colitis in a homosexual man.	this report describes a case of fulminant amebic colitis leading to perforation and death in a 35-yr-old homosexual man. although entamoeba histolytica may be isolated from stool specimens in 20 to 30% of selected homosexual populations, reports of severe or invasive disease are rare. some workers have suggested that amebae are only passive colonizers of the colon in homosexual men. in our patient, pathological examination confirmed the presence of extensive colitis with penetration of amebae th ...	1986	2869684
cysteine proteinase of entamoeba histolytica. i. partial purification and action on different enzymes.	a method for a 50-60-fold purification of a cysteine proteinase from trophozoites of entamoeba histolytica using 35-80% ammonium sulphate fractionation, gel chromatography on sephadex g-75, and preparative isoelectric focusing is described. the enzyme was examined for its proteolytic potencies towards native enzyme substrates. the amebic proteinase directly inactivates aldolase and glyceraldehyde-3-phosphate dehydrogenase from rabbit muscle as well as glucose-6-phosphate dehydrogenase from yeast ...	1986	2870430
cysteine proteinase of entamoeba histolytica. ii. identification of the major split position in bovine insulin b-chain.	the substrate specificity of a cysteine proteinase of entamoeba histolytica was tested using the oxidized b-chain of bovine insulin. 28 proteolytic peptides were formed after different intervals of incubation and could be separated by high-performance liquid chromatography. two dominant peptides appearing after 10 min of incubation were isolated and analyzed for amino acid composition. the results obtained indicate that these two peptides were formed by cleavage of the peptide bond between the a ...	1986	2870431
failure to establish experimental entamoeba histolytica infection in mongolian gerbils (meriones unguiculatus) naturally infected with entamoeba muris.		1985	2870571
stimulation of dna synthesis on animal cell microcultures by a heat-stable cytoplasmic factor of entamoeba histolytica.		1986	2870593
secondary cerebral amebiasis due to infection with entamoeba histolytica. a case report with computer tomographic findings.	the clinical and serological findings and computer tomographic (ct) appearances of a case of secondary cerebral amebiasis due to entamoeba histolytica are presented. the effect of therapy on the neurological symptoms as well as on the ct findings are described in detail. the multiple intracerebral lesions resolved almost completely and the patient survived without any neurological sequelae.	1986	2870923
use of periodate activated sepharose 4b immunoadsorbent in purification of anti entamoeba histolytica antibody.		1985	2870982
coproantibody study in entamoeba histolytica, entamoeba hartmanni and other parasitic infections.		1985	2870983
amoebic pericarditis.		1985	2871111
antibody-induced caps in entamoeba histolytica: isolation and electrophoretic analysis.	polyspecific antibodies bound to the cell surface of entamoeba histolytica are polarized toward the uroid region and spontaneously released by the amoeba as supramolecular aggregate or cap. we purified these exfoliated structures and analyzed them by electron microscopy and sodium dodecyl sulfate electrophoresis. isolated caps were mainly composed of membranes and contained five major [35s]methionine-labeled bands; many more bands appeared (together with immunoglobulins) after silver staining. s ...	1986	2871113
amebiasis: introduction, current status, and research questions.	this series of five papers on the worldwide problem of amebiasis, its current status, research needs, and opportunities for progress has grown out of a renewal of investigative interest in amebiasis. the recent development of in vitro culture methods and of means to distinguish strain differences and the application of modern tools of cellular biology and biochemistry have raised new questions regarding strain definitions, virulence traits, host defenses, and the mechanism of invasive disease so ...	1986	2871618
problems in recognition and diagnosis of amebiasis: estimation of the global magnitude of morbidity and mortality.	major problems with a wide array of imperfect tests for diagnosis of amebiasis severely limit the understanding of its magnitude and epidemiology. a greater hindrance is the varied, inconsistent application of existing methods in different areas of the world. the best estimates suggest that probably 480 million people were infected with entamoeba histolytica and 36 million developed disabling colitis or extraintestinal abscesses in 1981. at least 40 thousand deaths are attributable to amebiasis, ...	1986	2871619
immunologic tools in the diagnosis of amebiasis: epidemiology in the united states.	several immunodiagnostic tests that detect antibody to entamoeba histolytica are now available. such tests are useful when demonstration of the etiologic agent is difficult (as in liver abscess), when routine diagnostic tests fail to disclose the organisms (as in severe intestinal disease compromised by failure of the laboratory to identify the organism or by the patient's use of interfering substances), and as epidemiologic tools for determining the prevalence of invasive disease in populations ...	1986	2871620
pathogenesis of disease caused by entamoeba histolytica: studies of adherence, secreted toxins, and contact-dependent cytolysis.	amebiasis is due to invasion of the colonic mucosa by the enteric protozoan entamoeba histolytica. the biology of the parasite and the pathogenic mechanisms of e. histolytica have been extensively studied. recent studies provide new insights into the mechanism by which amebas colonize the gut, interact with bacterial flora, adhere to and lyse tissue, and combat the host's inflammatory response. elucidation of the biochemical and cellular bases of the pathogenesis of disease caused by e. histolyt ...	1986	2871621
review of the human immune mechanisms directed against entamoeba histolytica.	the human immune mechanisms effective against the destructive parasite entamoeba histolytica have not been established. humoral responses develop with invasive disease but have not positively correlated with protection against recurrent invasive infection. though complement is amebicidal, invasive strains of amebas may be resistant. polymorphonuclear neutrophils have not been active in vitro against virulent amebic strains and could contribute to the pathogenesis of disease. in vitro studies hav ...	1986	2871622
nonresolution of an amebic liver abscess after parasitologic cure.	a 43-year-old filipino male was admitted to a manila hospital with a 1 month history of epigastric pain and fever, and was found to have a palpable epigastric mass. computerized tomography revealed a large hepatic abscess which serologically was shown to be amebic. chemotherapy resulted in clinical cure and an initial reduction in size of the liver abscess. however, resolution of the abscess cavity did not occur, and on closed needle aspiration, 80 cc of characteristic amebic pus was recovered. ...	1986	2871768
fulminant amebic colitis.	nine patients with fulminating amebic colitis who were treated surgically from 1975 to 1982 are presented. only those who had bowel resections with exteriorization of the cut ends survived. the pertinent literature is reviewed briefly.	1986	2872021
specific circulating immune complexes in amoebic liver abscess.	a micro enzyme-linked immunosorbent assay was used to detect the presence of amoebic antigen in polyethylene-glycol-precipitated circulating immune complexes (cic). a cutoff dilution of greater than 1:160 for the precipitates was taken to be of clinical significance. among the patients with amoebic liver abscess, 93% (14 of 15 confirmed cases) had indications of amoebic antigen in cic. in 57% of the suspected cases of amoebic liver abscess, amoebic antigen could be detected in cic. the specifici ...	1986	2872230
the interaction of human neutrophils and entamoeba histolytica increases cytopathogenicity for liver cell monolayers.	the in vitro interaction of neutrophils and virulent axenic entamoeba histolytica trophozoites on chang liver cells was studied to determine if the presence of neutrophils enhanced destruction of liver cell monolayers. axenic amoebae (strain hm1:imss) destroy liver cell monolayers in a dose-dependent manner (p less than .001). human neutrophils had no effect on the liver cell monolayers; however, when neutrophils were added to amoebae, destruction of monolayers was enhanced (p less than .05 comp ...	1986	2872253
isolation and characterization of phagocytosis- and virulence-deficient mutants of entamoeba histolytica.	we have used chemical mutagenesis to isolate 10 independent phagocytosis-deficient mutants of entamoeba histolytica from a highly virulent clone (strain hm1:imss). phagocytosis-deficient mutants were characterized by several biologic properties related to amoebic pathogenic mechanisms: virulence, adhesion to red blood cells, cytopathic effect of live trophozoites on cell culture monolayers, ability of amoebic extracts to destroy cell culture monolayers, and growth in semisolid agar. all of these ...	1986	2872254
purine-metabolising enzymes in entamoeba histolytica.	the enzymes that catalyse the salvage of purines in entamoeba histolytica trophozoites have been surveyed. adenine deaminase (ec 3.5.4.2), adenosine deaminase (ec 3.5.4.4), guanine deaminase (ec 3.5.4.3), adenine phosphoribosyltransferase (prtase) (ec 2.4.2.7), xanthine prtase (ec 2.4.2.22) and hypoxanthine prtase (ec 2.4.2.8) were all detected in cell homogenates but only at low activities, whereas amp deaminase (ec 3.5.4.6) and guanine prtase (ec 2.4.2.8) were not found. phosphorylases (ec 2.4 ...	1986	2872591
non-ulcerative amebiasis of rectum.		1986	2872792
entamoeba histolytica: effect of growth conditions and bacterial associates on isoenzyme patterns and virulence.	in xenic culture, isolates of entamoeba histolytica from asymptomatic carriers are characterized, with rare exception, by possession of a nonpathogenic zymodeme. during the process of axenizing such an isolate, strain cdc:0784:4, a change in the pattern of the isoenzymes from nonpathogenic zymodeme i to pathogenic zymodeme ii was observed 40 days after the amebae had been transferred from a medium for xenic cultivation to one used for axenic cultivation, but before axenization of the amebae had ...	1986	2873049
production of mouse monoclonal antibodies which inhibit in vitro adherence of entamoeba histolytica trophozoites.	adherence by axenic entamoeba histolytica trophozoites to mammalian cells is mediated by an n-acetylgalactosamine (galnac)-inhibitable adhesin on the surface of the parasite. we isolated 35 hybridoma cell lines producing antibodies to e. histolytica as indicated by elisa with sonicated amebic protein or by immunofluorescence assay with fixed whole trophozoites. tissue culture supernatants were further screened for subcloning by the ability to bind to chinese hamster ovary (cho) cells which were ...	1986	2873102
the effect of colonic bilharzial lesions on the prevalence and invasiveness of intestinal amoebiasis.		1986	2873192
evaluation of different cultures used for cultivation of entamoeba histolytica in arab republic of egypt.		1986	2873193
ionophore a23187 stimulates entamoeba histolytica to release prostaglandin f2 alpha.	high performance liquid chromatography and radioimmunoassay were used to identify the prostaglandins synthesized by entamoeba histolytica (nih:200 strain). ca2+ionophore a 23187 stimulated amebae produced elevated levels of prostaglandin f2 alpha when compared with the control cells. there were no significant differences in the levels of prostaglandin e2 and thromboxane b2. this is the first report of a stimulated non-mammalian cell producing prostaglandin f2 alpha.	1986	2873586
[asymptomatic parasitic infection in infants under 6 months].		1985	2873630
[incidence of entamoeba histolytica in children with chronic diarrhea without macroscopic evidence of blood in the feces].		1986	2873654
in vitro activity of a substituted phenanthroline on entamoeba histolytica.		1986	2873667
comparative in vitro activity of mefloquine, diloxanide furoate and other conventionally used amoebicides against entamoeba histolytica.		1986	2874011
loss of susceptibility to complement lysis in entamoeba histolytica hm1 by treatment with human serum.	entamoeba histolytica hm1, exposed to a series of treatment with normal human serum (nhs), progressively lost susceptibility to complement lysis. trophozoites were incubated daily with unheated or heat-inactivated nhs for 2 hr at 36 degrees, starting with 10% v/v serum and increasing by 5% every 3 days up to 40% nhs. resistance to complement lysis was also obtained with two different hm1 clones but not with the low virulent strain hk9. induction of resistance dependent on the number of nhs treat ...	1986	2874111
antibody-dependent macrophage-mediated cytotoxicity against entamoeba histolytica.	interactions between trophozoites of entamoeba histolytica and peritoneal exudate macrophages from unsensitised and antigen-sensitised animals were studied in vitro. normal macrophages killed trophozoites to some extent. this killing capacity was enhanced by prior sensitisation of the animals with specific antigen. incorporation of anti-amoebic antiserum in the amoeba-macrophage mixture greatly enhanced the killing capacity of macrophages. fraction one (f-i) of a crude amoebic extract was most e ...	1986	2874228
amoebic abscess of spleen.		1986	2874240
entamoeba histolytica as a commensal intestinal parasite in homosexual men.	entamoeba histolytica is considered to be an uncommon, imported organism in the united kingdom and in many parts of north america, but recent attention has been drawn to the possibility of sexual transmission of this parasite among homosexual men. to determine the prevalence and clinical importance of enteric parasitic infections in men attending a clinic in london for the treatment of sexually transmitted diseases, we studied 354 randomly selected patients who provided a single stool sample tha ...	1986	2874484
isoenzyme patterns and pathogenicity in amebic infection.		1986	2874486
treatment of homosexual men infected with entamoeba histolytica.		1986	2874487
amebic infections in asymptomatic homosexual men, lack of evidence of invasive disease.	a survey for enteric infections in 140 asymptomatic homosexual men who attended a community clinic revealed a high prevalence of infection with entamoeba histolytica (27.1 per cent) and giardia lamblia (15.7 per cent). in contrast, the prevalence of elevated indirect hemagglutination (iha) titers (greater than or equal to 1:128), which indicate invasive amebiasis, was low (5.7 per cent). our findings suggest that only a limited amount of invasive amebic disease is occurring in this group of homo ...	1986	2874747
elucidation of cellular population and nature of anti-amoebic antibodies in cytotoxicity to entamoeba histolytica (nih:200).	interactions between trophozoites of entamoeba histolytica and cells of the immune system were studied in vitro by mixing effector cells obtained from normal and immunized guinea pigs with trophozoites in a ratio of 10:1. crude amoebic extract (cae) and its highest molecular weight (mw 650,000) fraction (f-i) were used for priming the effector cells in vivo. the effector cells were collected from the spleens of normal and immunized animals. lymphocytes were prepared by allowing splenic mononucle ...	1986	2875144
analysis of antigens from membrane and soluble fractions of entamoeba histolytica.	axenic cultures of entamoeba histolytica strains hk-9, hm-1, and rahman were fractionated to provide plasma membranes, internal, vesiculated membranes, and a soluble cytosol. each particulate fraction was solubilized and all fractions were analyzed by techniques designed to demonstrate molecular complexity and serologic reactivity. the cytosol contained more antigenic moieties than either membrane fraction; however, the antigens associated with the membranes had very high reactivity and lower no ...	1986	2875181
[research on helminth eggs and protozoan cysts on money].		1986	2875514
[enteropathogens in travellers returning from the tropics].	diarrhea is not only the most common health hazard during travel in the tropics but also the most frequent condition which prompts returning travellers to see a physician. the prevalence of bacterial and parasitic enteropathogens in people attending our outpatient department after returning from the tropics has been studied and the laboratory results of stool examinations have been compared with clinical symptoms. of the 173 persons enrolled, 19 (11%) harboured bacterial pathogens and pathogenic ...	1986	2875520
in vitro amoebicidal testing of natural products; part i. methodology.		1986	2876447
the detection of enteropathogens in acute diarrhea in a family cohort population in rural egypt.	in 8 villages of rural northeastern egypt, a 2-year study of the etiologic agents associated with episodes of diarrhea was carried out. stool specimens (3,243) from 3,513 episodes of diarrhea were processed for enteropathogens. the most commonly identified agents in the group with diarrhea were giardia lamblia (44%), heat stable enterotoxin (st)-producing enterotoxigenic escherichia coli (etec) (15%), heat labile toxin (lt)-producing etec (12%), enteropathogenic e. coli (epec) (4%), rotavirus (3 ...	1986	2876656
[measurement of oxidation-reduction potentials in the cultivation of entamoeba histolytica].		1986	2876762
in vitro antimicrobial effects of furazolidone & metronidazole.		1986	2876955
prevalence of enteric parasites in homosexual patients attending an outpatient clinic.	a total of 372 pooled stool specimens from 274 homosexual men with diarrhea were submitted for parasitologic examination over a 2.5-year period. each two-vial pooled specimen set contained portions of stool from 3 consecutive days in formalin and polyvinyl alcohol. of the 274 patients, 133 (48.5%) harbored one or more intestinal protozoa, with 161 (43.3%) of the 372 specimens submitted being positive for one or more organisms. the parasites identified included entamoeba histolytica (71 patients) ...	1986	2877006
on three stages of amoebic research.		1986	2877277
interaction of serum components with the cytotoxic action of entamoeba histolytica.	native normal human serum is capable of inhibiting the cytotoxic action of entamoeba histolytica against k562 tissue culture target cells assessed by a 51cr-release test. it is suggested that a part of the inhibitory activity on amoebae's cytotoxic action is represented by the complement system which is known to lyze trophozoites by activation of the alternative pathway. for the rest of the serum's inhibitory activity on amoebic cytotoxic action molecule(s) is (are) responsible which act(s) inde ...	1986	2877551
factors contributing to the pathogenic behavior of entamoeba histolytica.		1986	2877612
impaired production of alpha and gamma interferon in asymptomatic homosexual males.	in vitro production of alpha interferon and gamma interferon was examined in cell cultures from 90 asymptomatic homosexual males and 19 healthy heterosexual male controls. the production of alpha and gamma interferon was significantly suppressed in homosexuals as compared to that in heterosexual controls (p less than 0.005 and p less than 0.001, respectively). forty-one of the homosexuals produced less gamma interferon than any of the heterosexual controls. antibodies against the human immune sy ...	1986	2877877
changes in isoenzyme patterns of a cloned culture of nonpathogenic entamoeba histolytica during axenization.	the axenization of an entamoeba histolytica isolate with a nonpathogenic isoenzyme electrophoretic pattern (zymodeme) was recently achieved for the first time (15). forty days after the cells were transferred to the medium used for axenic cultivation, the amebae developed virulence properties, and the zymodeme converted to a pathogenic pattern. to exclude the possibility that the original isolate consisted of two zymodeme populations and that conditions of growth selected for a particular popula ...	1986	2877951
amoeba research and history.		1986	2878270
macrophage spreading inhibition test as an alternate method for in vitro assessment of cell-mediated immunity against entamoeba histolytica.	during cellular immune responses sensitized lymphocytes release a number of mediators collectively known as 'lymphokines'. the assaying of macrophage spreading inhibition (msi) factor released by sensitized lymphocytes was employed as an alternative procedure for in vitro detection of cell-mediated immunity (cmi) in animals immunized with amoeba antigen preparation. the msi test was compared with macrophage migration inhibition test. a well defined cmi was detectable in animals immunized by amoe ...	1986	2878487
immunodiagnosis of entamoeba histolytica and ascaris lumbricoides infections in caucasian and aboriginal australians.	the immunodiagnostic efficiency of an indirect immunofluorescence test (ifat) and in vitro lymphocyte proliferative responsiveness (cell mediated immunity test, cmit) used to measure the immunological responses of individuals with known natural entamoeba histolytica and ascaris lumbricoides infections, was studied under survey conditions. e. histolytica was common among aborigines from cherbourg, kowanyama and central australia, but it was not found in brisbane caucasians. the protozoan was sele ...	1986	2878514
isoenzyme patterns of entamoeba histolytica isolates from asymptomatic carriers: use of gradient acrylamide gels.	a vertical polyacrylamide gradient gel (3% to 7%) was designed to facilitate the electrophoretic resolution and classification of isoenzyme patterns of entamoeba histolytica isolates. the following enzyme systems were used: phosphoglucomutase (pgm), hexokinase (hx), glucosephosphate isomerase (gpi), and malate dehydrogenase (me). the modifications in the electrophoretic procedure and sample preparation allowed the reproducible comparison of enzyme patterns of axenic, monoxenic, and mixed culture ...	1986	2878625
longitudinal study of diarrhoeal disease in a peri-urban community in manaus (amazon-brazil).	a 20-month longitudinal study of diarrhoeal disease was carried out in a poor peri-urban community of manaus (amazon-brazil), and the attack rate of this disease ranged from 0.2 to 4.8 episodes of diarrhoea per person per year. the age group most affected was 0 to 35 months old. a probable aetiological agent was identified in 68 of the 110 faecal samples collected. the most frequent enteropathogens isolated were enterotoxigenic escherichia coli and giardia lamblia. enterotoxigenic escherichia co ...	1986	2878647
a membrane-associated neuraminidase in entamoeba histolytica trophozoites.	trophozoites of the parasitic amoeba entamoeba histolytica hm-1:imss possess a surface neuraminidase capable of liberating n-acetylneuraminic acid (nana) from n-acetylneuramin-lactose (alpha 2----3 or alpha 2----6) or mucin in their medium. the neuraminidase was found to be membrane associated, with more than 50% of the yield being recovered in the plasma membrane fraction. the neuraminidase specific activity of the plasma membrane fraction was six times that of internal membrane fraction enzyme ...	1987	2878886
[intestinal parasitic diseases of school children in the republic of niger].	coprologic surveys realized in schoolchildren in a few western areas of the republic of niger provided the following results. concerning protozoa, the prevalence of amebiasis-infestation is strong in all the studies areas, giardiasis is more frequent in dry areas. as for the helminths, hymenolepsis nana is met everywhere, the prevalence of ancylostomiasis decreases gradually from the south to the north and the endemic area of schistosoma mansoni is limited to the extreme south of the country. th ...	1986	2879647
[in vitro effect of tinidazole on entamoeba histolytica].		1986	2879651
[molecular mechanisms in the pathogenicity of entamoeba histolytica].		1986	2879652
amoebic research.		1987	2880001
prevalence of amoebic antibody in population affected by epidemic non-a, non-b hepatitis.		1987	2880253
protection of mice against intestinal amoebiasis with bcg, corynebacterium parvum and listeria monocytogenes.	treatment with corynebacterium parvum or bcg, or infection with live listeria monocytogenes was found to protect mice against subsequent infection with entamoeba histolytica. complete protection was obtained in mice treated with 10(7) (colony forming units) of bcg but not with 10(5). partial protection was achieved with 10(6) of bcg. these data provide evidence that non-specific immunity plays an important role in the host defense against amoebic infection.	1986	2880331
in vivo effect of histamine & acetylcholine on the virulence of entamoeba histolytica.		1986	2880802
amoebae research.		1987	2881059
antiamebic antibodies in homosexual men.		1987	2881205
human amoebiasis: cultivation and experimental transmission in animals.	in vitro cultivation was carried out on an initial strain of entamoeba histolytica from human source. dysphasic medium was used and concentrations of horse serum and antibiotic varied in response to developments. experimental infection of dogs, rabbits and mice was carried. except for the controls, all animals were orally infected and monitored until the experiment was terminated. parasitaemia became evident as from the 6th day in dogs experimentally infected and until about the 20th day when lo ...	1986	2881905
mitogenic factor for t inducer/helper cells in entamoeba histolytica extracts.		1985	2882454
comparative inflammatory reactions in mice and hamsters to entamoeba histolytica.		1987	2882461
entamoeba histolytica: cytopathogenicity and lectin activity of avirulent mutants.	three clones of entamoeba histolytica (l-6, c93, c919) were isolated by mutagenesis with ethyl methanesulfonate from the axenic strain hm1:imss and were studied for adherence, cytolytic, and soluble galactose inhibitable lectin activity. avirulent clones adhered to and killed fewer chinese hamster ovary cells than hm1:imss (p less than 0.01). however, only c919 was deficient in adherence to red blood cells. galactose (1.0 g) completely inhibited adherence of all the mutants to chinese hamster ov ...	1987	2883020
virulence of entamoeba histolytica correlates with the capacity to develop complement resistance.		1986	2883155
lysozyme and complement-dependent lysis of entamoeba histolytica. inhibition by leupeptin.		1986	2883156
induction of protective immunity to experimental hepatic amoebic infection in hamsters.	immunisation of hamsters with the sephadex g-200 chromatographed fraction-i of the crude amoebic extract afforded protection in 58% of the animals against an intrahepatic challenge with a virulent subline of axenic entamoeba histolytica nih-200 (v). the protected animals had variable levels of anti-fi antiamoebic antibodies, while none of the infected controls had such antibodies. findings suggest that chromatographed fi of crude amoebic extract might function as a potent immunogen affording pro ...	1987	2883233
localization and identification of an entamoeba histolytica adhesin.	the adherence of entamoeba histolytica trophozoites to target cells was studied by using monoclonal antibodies (mabs) and adhesion-deficient mutants of the parasite. mabs adh-1 and adh-2 reacted with a surface protein of approximately 112 kda of the total proteins of trophozoites from the wild type strain, clone a, strain hm1:imss. both mabs reacted weakly with the adhesion-deficient mutant clones, c-98, c-919 and c-923, all derived from hm1:imss. mabs adh-1 and adh-2 incubated with trophozoites ...	1987	2883572
genomic and cdna actin sequences from a virulent strain of entamoeba histolytica.	invasiveness of entamoeba histolytica strains that cause acute amoebiasis is characterized by aggressive behavior associated with cell motility and actin function. analysis of actin genes from e. histolytica was initiated by devising methods for the isolation of biologically active nucleic acids, which allowed the preparation of cdna and genomic dna libraries. e. histolytica actin-encoding cdnas and genomic clones have been isolated from libraries prepared from the virulent hm1:imss strain using ...	1987	2883657
in vitro studies on the sensitivity of local entamoeba histolytica to anti-amoebic drugs.	the in vitro activity of drugs, namely dehydroemetine, ornidazole, metronidazole and tinidazole were determined against the locally isolated strains of e. histolytica in thailand. the test was performed in liquid monophasic medium, i.e. liver marmite serum medium. in all, locally isolated strains from thirty hosts studied, the minimal inhibitory concentration (mic) for dehydroemetine ranged from 0.125 to 1 microgram/ml, ornidazole ranged from 0.0625 to 0.25 microgram/ml, metronidazole ranged fro ...	1986	2883732
studies of the antigenic activity of entamoeba histolytica extracts.		1986	2884001
indications for treatment.		1987	2884194
partial characterization of dna-dependent rna polymerases from entamoeba histolytica.		1986	2884943
[analysis of the organization of the dna of various strains and clones of entamoeba histolytica].		1986	2884944
[development of a basic material for an axenic culture medium for entamoeba histolytica. ii. peptones].		1986	2884945
isolation and initial characterization of dna from entamoeba histolytica hm1:imss.		1986	2884946
high salt sds-dep isolation of rna from entamoeba histolytica: the recovery of intact 25s rrna from amebae of different culture ages and the co-isolation of dna.		1986	2884947
[castela texana: screening for its anti-amoebic activity].		1986	2884948