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changes in the ecological properties of organic wastes during their biological treatment.organic wastes, such as the organic fractions of municipal solid waste (ofmsw) or sewage sludge (ss), have become a serious environmental problem in russia as well as in other countries. the use of these wastes as soil amendments allows their negative impact on the environment to be minimized. however, before these wastes can be used, they need to be treated appropriately in order to decrease their level of hazard. in this study, composting of raw ss, ofmsw, a mixture of these two wastes (ofmsw+ ...201627692790
photoadduct formation from the fmn singlet excited state in the lov2 domain of chlamydomonas reinhardtii phototropin.the two light, oxygen, and voltage domains of phototropin are blue-light photoreceptor domains that control various functions in plants and green algae. the key step of the light-driven reaction is the formation of a photoadduct between its fmn chromophore and a conserved cysteine, where the canonical reaction proceeds through the fmn triplet state. here, complete photoreaction mapping of crlov2 from chlamydomonas reinhardtii phototropin and aslov2 from avena sativa phototropin-1 was realized by ...201627766868
rhizosphere organic anions play a minor role in improving crop species' ability to take up residual phosphorus (p) in agricultural soils low in p availability.many arable lands have accumulated large reserves of residual phosphorus (p) and a relatively large proportion of soil p is less available for uptake by plants. root released organic anions are widely documented as a key physiological strategy to enhance p availability, while limited information has been generated on the contribution of rhizosphere organic anions to p utilization by crops grown in agricultural soils that are low in available p and high in extractable ca, al, and fe. we studied t ...201627872635
compromised photosynthetic electron flow and h2o2 generation correlate with genotype-specific stomatal dysfunctions during resistance against powdery mildew in oats.stomatal dysfunction known as "locking" has been linked to the elicitation of a hypersensitive response (hr) following attack of fungal pathogens in cereals. we here assess how spatial and temporal patterns of different resistance mechanisms, such as hr and penetration resistance influence stomatal and photosynthetic parameters in oat (avena sativa) and the possible involvement of hydrogen peroxide (h2o2) in the dysfunctions observed. four oat cultivars with differential resistance responses (i. ...201627877184
key phytochemicals contributing to the bitter off-taste of oat (avena sativa l.).sensory-directed fractionation of extracts prepared from oat flour (avena sativa l.) followed by lc-tof-ms, lc-ms/ms, and 1d/2d-nmr experiments revealed avenanthramides and saponins as the key phytochemicals contributing to the typical astringent and bitter off-taste of oat. besides avenacosides a and b, two previously unreported bitter-tasting bidesmosidic saponins were identified, namely, 3-(o-α-l-rhamnopyranosyl(1→2)-[β-d-glucopyranosyl(1→3)-β-d-glucopyranosyl(1→4)]-β-d-glucopyranosid)-26-o-β ...201627983806
the impact of oat (avena sativa) consumption on biomarkers of renal function in patients with chronic kidney disease: a parallel randomized clinical trial.animal studies report that oat (avena sativa l) intake has favorable effects on kidney function. however, the effects of oat consumption have not been assessed in humans. the aim of this study was to examine the impact of oat intake on biomarkers of renal function in patients with chronic kidney disease (ckd).201628003041
conversion of oat (avena sativa l.) haploid embryos into plants in relation to embryo developmental stage and regeneration media.obtaining oat dh lines is only effective via wide crossing with maize. seven hundred haploid embryos from 21 single f1 progeny obtained from wide crosses with maize were isolated, divided into four groups according to their size (<0.5 mm, 0.5-0.9 mm, 1.0-1.4 mm, and ≥1.5 mm), and transferred into 190-2 regeneration medium with different growth regulators: 0.5 mg l(-1) kinetin (kin) and 0.5 mg l(-1) 1-naphthaleneacetic acid (naa); 1 mg l(-1) zeatin (zea) and 0.5 mg l(-1) naa; or 1 mg l(-1) dicamb ...201628042230
femtosecond to millisecond dynamics of light induced allostery in the avena sativa lov domain.the rational engineering of photosensor proteins underpins the field of optogenetics, in which light is used for spatiotemporal control of cell signaling. optogenetic elements function by converting electronic excitation of an embedded chromophore into structural changes on the microseconds to seconds time scale, which then modulate the activity of output domains responsible for biological signaling. using time-resolved vibrational spectroscopy coupled with isotope labeling, we have mapped the s ...201728068090
laboratory silo type and inoculation effects on nutritional composition, fermentation, and bacterial and fungal communities of oat silage.the objectives were to evaluate (1) the use of 2 types of experimental silos (s) to characterize whole-crop oat (avena sativa l.) silage with or without addition of an inoculant (i), and (2) the effect of inoculation on the microbial community structure of oats ensiled using only plastic bucket silos (bkt). from each of 6 sections in a field, oats were harvested, treated (ino) or not (con) with inoculant, packed into 19-l bkt or vacuum bags (bg), and ensiled for 217 d. the inoculant added contai ...201728088418
advances on the determination of thresholds of cu phytotoxicity in field-contaminated soils in central chile.to better determine phytotoxicity thresholds for metals in the soil, studies should use actual field-contaminated soil samples rather than metal-spiked soil preparations. however, there are surprisingly few such data available for cu phytotoxicity in field-contaminated soils. moreover, these studies differ from each other with regards to soil characteristics and experimental setups. this study aimed at more accurately estimating cu phytotoxicity thresholds using field-collected agricultural soil ...201728131477
oat doubled haploids following maize pollination.doubled haploids (dhs) are an important tool for the accelerated production of new crop varieties. in oat, dhs were first produced by pollinating oat florets with maize pollen. the resultant embryos spontaneously eliminate the maize chromosomes leaving a haploid complement of oat chromosomes. these embryos can be cultured in vitro using the "embryo rescue" technique to produce haploid plants whose chromosome number can be doubled with colchicine to produce homozygous dh oat plants.201728132140
wide hybridization between oat and pearl millet.wide hybridization is a one of the important techniques in plant breeding. oat (avena sativa l.) and pearl millet (pennisetum glaucum l.) belong to different subfamilies of poaceae. in generally, such distant relative species show uniparental chromosome elimination after successful fertilization. however, all seven pearl millet chromosomes are retained beside the genome of oat during embryogenesis. hybrid seedlings develop, but show necrosis after light irradiation. here, a detailed protocol for ...201728132141
protocol for producing synthetic polyploid oats.a protocol to produce synthetic oats by introducing alien genetic variations into cultivated oats is proposed based on suitable genetic relationships between avena species. furthermore, artificial hybridization procedures between different ploidy species are explained. amphiploids can be produced by rescuing aborted embryos and treating the f1 hybrids with colchicine to overcome sterility between interspecific plants. furthermore, i present the cytological methods for observing somatic and meiot ...201728132142
oat anther culture and use of dh-lines for genetic mapping.possibility to make doubled haploids (dhs) from varying crossing populations is a useful tool for enhancing cultivar breeding, and a source of valuable material for genetic research. oats is reported to be recalcitrant in anther culture with low response and genotype dependency. however, the best recoveries reported have reached up to 30 green regenerants per 100 isolated anthers, which clearly addresses the potential of this technique. in this chapter, one successful oat anther culture protocol ...201728132144
m13-tailed simple sequence repeat (ssr) markers in studies of genetic diversity and population structure of common oat germplasm.simple sequence repeat (ssr) markers are one of the most frequently used molecular markers in studies of crop diversity and population structure. this is due to their uniform distribution in the genome, the high polymorphism, reproducibility, and codominant character. additional advantages are the possibility of automatic analysis and simple interpretation of the results. the m13 tagged pcr reaction significantly reduces the costs of analysis by the automatic genetic analyzers. here, we also dis ...201728132150
isolation of oat (avena sativa l.) total proteins and their prolamin fractions for 2d electrophoresis.appropriate sample preparation is essential to obtaining good results of two-dimensional gel electrophoresis (2-de). for various reasons (particularly phenolic compounds, proteolytic enzymes, and cell-wall mucilages) the extraction of proteins from plant material, among them oat proteins, is difficult. during isolation all soluble substances that may interfere with the analysis (especially isoelectric focusing) are removed, and proteins of interest are separated from the remains. however, the ap ...201728132154
2-de separation and identification of oat (avena sativa l.) proteins and their prolamin fractions.at present two-dimensional polyacrylamide gel electrophoresis (2-de) is the most widely used proteomic tool, which enables simultaneous separation of even thousands of proteins with a high degree of resolution. the quality of 2-de separation depends on the type of biological material used as a protein source. the presence of interfering compounds (e.g., phenols, as it is the fact in plant material including oat seeds) impedes 2-de run. with the use of this technique it is possible to analyze the ...201728132155
free asparagine and sugars profile of cereal species: the potential of cereals for acrylamide formation in foods.cereals-based food is one of the major source of maillard reaction products in the diet. free amino acids and reducing sugars are considered to be the main precursors in the formation of these heat-induced compounds. in order to determine genetic resources with reduced potential for acrylamide formation, the content of sugars as well as free asparagine were analysed in a total of 30 cultivars of 10 varieties belonging to eight species (triticum aestivum var. lutescens, t. aestivum var. alba, t. ...201728150529
chromosomal distribution patterns of the (ac)10 microsatellite and other repetitive sequences, and their use in chromosome rearrangement analysis of species of the genus avena.fluorescence in situ hybridization (fish) was used to determine the physical location of the (ac)10 microsatellite in metaphase chromosomes of six diploid species (aa or cc genomes), two tetraploid species (aacc genome), and five cultivars of two hexaploid species (aaccdd genome) of the genus avena, a genus in which genomic relationships remain obscure. a preferential distribution of the (ac)10 microsatellite in the pericentromeric and interstitial regions was seen in both the a- and d-genome ch ...201728156137
unraveling the evolutionary dynamics of ancient and recent polypoidization events in avena (poaceae).understanding the diversification of polyploid crops in the circum-mediterranean region is a challenging issue in evolutionary biology. sequence data of three nuclear genes and three plastid dna fragments from 109 accessions of avena l. (poaceae) and the outgroups were used for maximum likelihood and bayesian analyses. the evolution of cultivated oat (avena sativa l.) and its close relatives was inferred to have involved ancient allotetraploidy and subsequent recent allohexaploidy events. the cr ...201728157193
investigations of human myosin vi targeting using optogenetically controlled cargo loading.myosins play countless critical roles in the cell, each requiring it to be activated at a specific location and time. to control myosin vi with this specificity, we created an optogenetic tool for activating myosin vi by fusing the light-sensitive avena sativa phototropin1 lov2 domain to a peptide from dab2 (lovdab), a myosin vi cargo protein. our approach harnesses the native targeting and activation mechanism of myosin vi, allowing direct inferences on myosin vi function. lovdab robustly recru ...201728193860
pyricularia graminis-tritici, a new pyricularia species causing wheat blast.pyricularia oryzae is a species complex that causes blast disease on more than 50 species of poaceous plants. pyricularia oryzae has a worldwide distribution as a rice pathogen and in the last 30 years emerged as an important wheat pathogen in southern brazil. we conducted phylogenetic analyses using 10 housekeeping loci for 128 isolates of p. oryzae sampled from sympatric populations of wheat, rice, and grasses growing in or near wheat fields. phylogenetic analyses grouped the isolates into thr ...201628232765
residual soil nitrate content and profitability of five cropping systems in northwest iowa.many communities in the midwestern united states obtain their drinking water from shallow alluvial wells that are vulnerable to contamination by no3-n from the surrounding agricultural landscape. the objective of this research was to assess cropping systems with the potential to produce a reasonable return for farmers while simultaneously reducing the risk of no3-n movement into these shallow aquifers. from 2009 to 2013 we conducted a field experiment in northwest iowa in which we evaluated five ...201728248976
antiproliferative activity of vitexin-2-o-xyloside and avenanthramides on caco-2 and hepg2 cancer cells occurs through apoptosis induction and reduction of pro-survival mechanisms.caco-2 colon cancer cells and hepg2 liver cancer cells represent two malignant cell lines, which show a high resistance to apoptosis induced by the conventional anticancer drugs. vitexin-2-o-xyloside (xvx) and avenanthramides (avns) are naturally occurring dietary agents from beta vulgaris var. cicla l. and avena sativa l., respectively. the aim of this work was to evaluate the antiproliferative effects and the reduction of the pro-survival mechanisms exerted by xvx and avns, used individually a ...201728283822
neighbour presence, not identity, influences root and shoot allocation in pea.competition is a key feature that structures the composition of plant communities. a growing body of evidence is showing that the presence of neighbours, especially belowground neighbours, induces varied morphological responses in plants. however, in many species, it is not known whether neighbour identity also influences plant morphological responses such as biomass allocation patterns. to assess plant response to above- and belowground neighbour presence and identity, we conducted a greenhouse ...201728291827
extraction, chemical characterization and antioxidant activity of litchi chinensis sonn. and avena sativa l. seeds extracts obtained from pressurized n-butane.the extraction of litchi (litchi chinensis sonn.) and oat (avena sativa l.) seeds were investigated using n-butane as pressurized solvent by evaluating the effect of pressure in the range of 7-100 bar and temperature from 25 to 70 °c on the extract yield and chemical composition together with the antioxidant activity of the extracts obtained. it was experimentally observed extraction yields for both seeds up to ~3.5 wt%, with a total phenolic content around 126.4 mg gae/100 g of extract, and an ...201728298700
biomass production of c3- and c4-plant species in pure and mixed culture with different water supply.pure and mixed cultures of the dicotyledons atriplex hortensis l. (c3 plant) and amaranthus retroflexus l (c4) on the one hand and of the grasses avena sativa l (c3) and panicum miliaceum l. (c4) on the other hand were maintained in a standard soil with different ground water tables. after 12 weeks the length, dry weight and nitrogen-content of the aboveground and belowground parts of the plants, and in addition the carbon-and ash-content and the δ(13)c value of the aboveground parts were determ ...198128310064
role of mycorrhizal infection in the growth and reproduction of wild vs. cultivated plants : i. wild vs. cultivated oats.we tested the hypothesis that mycorrhizal infection benefits wild plants to a lesser extent than cultivated plants. this hypothesis stems from two observations: (1) mycorrhizal infection improves plant growth primarily by increasing nutrient uptake, and (2) wild plants often possess special adaptations to soil infertility which are less pronounced in modern cultivated plants. in the first experiment, wild (avena fatua l.) and cultivated (a. sativa l.) oats were grown hydroponically at four diffe ...198828311275
biomass production and nitrogen content of c3- and c4- grasses in pure and mixed culture with different nitrogen supply.two c3 grasses (hordeum vulgare l., avena sativa l.) and two c4 grasses (panicum miliaceum l., panicum crus-galli l.) were cultivated in standard soil in the open air in pure cultures and in various mixed cultures at low and high nitrogen fertilization levels. after three months the dry weight, length and nitrogen content of the aboveground and below-ground parts of the plants and the shoot/root ratios were determined. hordeum vulgare was the most successful species irrespective of the nitrogen ...198728312237
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