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monitoring the binding processes of black tea thearubigin to the bovine serum albumin surface using quartz crystal microbalance with dissipation monitoring.the binding processes of thearubigin, which is one of the two major polyphenols (the other one is theaflavin) that gives black tea its characteristic color and taste, to the bovine serum albumin (bsa) surface have been investigated by quartz crystal microbalance with dissipation monitoring (qcm-d). the mass and thickness of the thearubigin adlayer on bsa surfaces at various thearubigin concentrations, salt concentrations, and ph values have been determined by qcm-d using the voigt model. our res ...200718031008
polymeric black tea polyphenols inhibit 1,2-dimethylhydrazine induced colorectal carcinogenesis by inhibiting cell proliferation via wnt/beta-catenin pathway.tea polyphenols like epigallocatechin gallate and theaflavins are established chemopreventive agents for colorectal carcinogenesis. however, studies on evaluating similar chemopreventive properties of thearubigins or polymeric black tea polyphenols (pbps), the most abundant polyphenols in black tea, are limited. hence, in the present study we aim to investigate chemopreventive effects along with probable mechanisms of action of pbp extract employing 1,2-dimethylhydrazine (dmh)-induced colorectal ...200818037152
comparative evaluation of antiproliferative, antiangiogenic and apoptosis inducing potential of black tea polyphenols in the hamster buccal pouch carcinogenesis model.to evaluate the relative chemopreventive efficacy of two black tea polyphenols, polyphenon-b [p-b] and btf-35 on 7,12-dimethylbenz [a]anthracene (dmba)-induced hamster buccal pouch (hbp) carcinogenesis.200718053169
quasi-flow injection analysis for rapid determination of caffeine in tea using the sample pre-treatment method with a cartridge column filled with polyvinylpolypyrrolidone.the sample pre-treatment method using a polyvinylpolypyrrolidone (pvpp) cartridge column combined with a quasi-flow injection analysis (quasi-fia) system realized the rapid determination of caffeine in three types of tea, green, oolong and black tea. the measurement time for each tea sample pre-treated using a pvpp cartridge column was shortened to 20s. in the present system, the limits of detection and quantification were 0.3 microm (1.5 pmol injected) and 0.7 microm (3.5 pmol injected), respec ...200818054948
structures of epicatechin gallate trimer and tetramer produced by enzymatic oxidation.during black tea production, catechins and their galloyl esters are enzymatically oxidized to generate a complex mixture of black tea polyphenols. the role of galloyl ester groups in this process has yet to be determined. enzymatic oxidation of epicatechin 3-o-gallate (1) yielded two new oxidation products, theaflavate c and bistheaflavate a, along with theaflavate a (2), a known dimer of 1 generated by coupling of the b-ring with the galloyl group. theaflavate c is a trimer of 1 and possesses t ...200718057757
both aluminum and polyphenols in green tea decoction (camellia sinensis) affect iron status and hematological parameters in rats.green tea leaves naturally contain high levels of polyphenols and aluminum (al). polyphenols in green tea decoction are considered to be one of the major factors responsible of low iron status. however, the effects of al from green tea decoction on iron status and hematological parameters remained unclear.200718060378
combination of hsccc and sephadex lh-20 methods an approach to isolation and purification of the main individual theaflavins from black tea.in order to separate the main individual theaflavin monomers from black tea, high-speed countercurrent chromatography (hsccc) and sephadex lh-20 column chromatography were applied. the results showed that theaflavin (tf1), theaflavin-3-gallate (tf2a), theaflavin-3'-gallate (tf2b) and theaflavin-3,3'-digallate (tf3) can be obtained by hsccc using a solvent system composed of n-hexane-ethyl acetate-methanol-water (1:3:1:6, v/v/v/v), but the tf1 was containing epicatechin-3-gallate (ecg). similarly ...200818063426
cloning and characterization of a cytosolic glutamine synthetase from camellia sinensis (l.) o. kuntze that is upregulated by aba, sa, and h2o2.a cdna encoding glutamine synthetase, one of the enzymes of the gs/gogat pathway, was cloned from camellia sinensis (csgs). the isolated cdna consists of 1,071 nucleotides encoding a polypeptide of 356 amino acids with an estimated isoelectric point of 6.13. the recombinant protein purified from escherichia coli using ni-nta affinity chromatography showed molecular mass of 39.2 kda. the purified protein was confirmed by blotting with anti-his antibodies. catalytic parameters of the protein were ...200818074244
assessment of fluoride concentration and daily intake by human from tea and herbal infusions.the fluoride content in infusions of commercially available black, green, oolong, pu-erh and white teas was determined by ion-selective electrode. herbal infusions as well as instant tea and ready-to-drink tea beverages were also examined. it is found that brewing time (5, 10 and 30 min) does increase the fluoride content, which in infusions of black tea (5 min brewing) was higher than that in the other types of tea, with contents ranging between 0.32 and 4.54 mg/l for black tea to 0.37-0.54 mg/ ...200818078704
epigallocatechin-3-gallate inhibits interleukin-1beta-induced muc5ac gene expression and muc5ac secretion in normal human nasal epithelial cells.it has been reported that the proinflammatory cytokine interleukin-1beta (il-1beta) induces mucus hypersecretion in normal human nasal epithelial (nhne) cells and that the map kinase pathway may be an important signal pathway in il-1beta-induced muc5ac gene expression. green tea (camellia sinensis) polyphenols are potent anti-inflammatory agents and have been shown to inhibit inflammation in tumor cell lines and cultured respiratory epithelial cells. in this study, we examined the effect of (-)- ...200818155512
differential effects of black versus green tea on risk of parkinson's disease in the singapore chinese health study.data from asian populations on dietary and lifestyle factors associated with parkinson's disease are sparse. in 1993-2005, the authors examined these factors in relation to parkinson's disease in the singapore chinese health study, a prospective cohort of 63,257 chinese men and women. baseline data were collected through in-person interviews using structured questionnaires. all 157 incident parkinson's disease cases were identified either through follow-up interviews or via linkage with hospital ...200818156141
effect of nr-anx-c (a polyherbal formulation) on haloperidol induced catalepsy in albino mice.use of typical antipsychotics like haloperidol in treatment of schizophrenia is associated with a high incidence of extrapyramidal side effects. in rodents, administration of haloperidol leads to the development of a behavioural state called catalepsy, in which the animal is not able to correct an externally imposed posture. in the present study we evaluated the anticataleptic efficacy of nr-anx-c, a polyherbal formulation containing bioactives of withania somnifera, ocimum sanctum, camellia sin ...200718160755
selective microbiologic effects of tea extract on certain antibiotics against escherichia coli in vitro.this study evaluated the microbiologic effects of black tea, compared to green tea, alone and in conjunction with selected antibiotics against escherichia coli, the common cause of intestinal and urinary tract infections.200718166124
[functional saponins in tea flower (flower buds of camellia sinensis): gastroprotective and hypoglycemic effects of floratheasaponins and qualitative and quantitative analysis using hplc].as a part of our characterization studies on the bioactive saponin constituents of tea flowers (camellia sinensis, flower buds), the methanolic extract and 1-butanol-soluble portion (the saponin fraction) from the flower buds were found to exhibit potent inhibitory effects on ethanol- and indomethacin-induced gastric mucosal lesions in rats and on serum glucose elevation in sucrose-loaded rats. among the constituents of the 1-butanol-soluble portion, floratheasaponins a, b, and c showed gastropr ...200818176066
determination of in vitro antidiabetic effects, antioxidant activities and phenol contents of some herbal teas.in this research, some herbal teas and infusions traditionally used in the treatment of diabetes in turkey, have been studied for their antidiabetic effects on in vitro glucose diffusion and phenolic contents and antioxidant activities. ten aqueous herbal tea extracts were examined using an in vitro method to determine their effects on glucose movement across the gastrointestinal tract. total phenol content of herbal teas was analyzed by folin-ciocalteu's procedure. antioxidant activities of her ...200818183488
role of quercetin (a natural herbal compound) in allergy and inflammation.an investigated flavonoid, quercetin, is reviewed in this article. quercetin is a bioflavonoid found in red wine, grapefruit, onions, apples, black tea, and, in lesser amounts, in leafy green vegetables and beans. quercetin has an antioxidant and anti-inflammatory activity and prevents cancer. quercitin inhibits the growth of certain malignant cells in vitro, and histamine and most cyclin-dependent kinases and also displays unique anticancer properties. quercetin is a natural compound that block ...200818187018
black tea-induced amelioration of hepatic oxidative stress through antioxidative activity in eac-bearing mice.it is recognized that during cancer, the disease itself as well as many of the anticancer drugs in use produce undesirable side effects such as hepatotoxicity. we have already demonstrated the antitumor and immunorestoring effects of black tea. here we report ehrlich's ascites carcinoma (eac)induced hepatotoxicity and its protection by antitumor dose of black tea in mice. hepatotoxicity was adjudged by liver histopathology and by measurement of plasma level of alkaline phosphatase (alp). an atte ...200718197822
in vitro mitigation of arsenic toxicity by tea polyphenols in human lymphocytes.the groundwater arsenicals have brought dreadful misery for the people residing in the endemic regions of west bengal, india. arsenic-related anomalies include arsenicosis, hyperkera-tosis, gastric complications, liver fibrosis, peripheral neuropathy, and cancer. some of these diseases have been frequently associated with overproduction of reactive oxygen species that cause dna damage and improper functioning of body's antioxidant defense mechanism. natural polyphenols present in tea serve as ex ...200718197836
effect of species variation and processing on phenolic composition and in vitro antioxidant activity of aqueous extracts of cyclopia spp. (honeybush tea).the in vitro antioxidant activity of aqueous extracts prepared from four cyclopia spp. (unfermented and fermented) was assessed using radical (abts *+) scavenging, ferric ion reduction, and inhibition of fe2+-induced microsomal lipid peroxidation as criteria. aqueous extracts of unfermented and fermented aspalathus linearis (rooibos) and camellia sinensis teas (green, oolong, and black) were included as reference samples. qualitative and quantitative differences in phenolic composition were demo ...200818198832
comparison of antioxidant potency of commonly consumed polyphenol-rich beverages in the united states.a number of different beverage products claim to have antioxidant potency due to their perceived high content of polyphenols. basic and applied research indicates that pomegranate juice (pj), produced from the wonderful variety of punica granatum fruits, has strong antioxidant activity and related health benefits. although consumers are familiar with the concept of free radicals and antioxidants, they are often misled by claims of superior antioxidant activity of different beverages, which are u ...200818220345
differential expression of histone h3 gene in tea (camellia sinensis (l.) o. kuntze) suggests its role in growing tissue.histone proteins are integral part of chromatin and their expression is typically linked to dna replication in the s phase of cell cycle. histone h3 is one of the four histones, along with h2a, h2b and h4, which forms the eukaryotic nucleosome octomer core. using differential display of mrna and rapid amplification of cdna ends (race), a full-length histone h3.1 cdna (csh3) was isolated from tea leaves. the open reading frame consisted of 411 nucleotides and deduced amino acid sequence comprised ...200918224457
tea polyphenols, their biological effects and potential molecular targets.tea is the most popular beverage in the world, second only to water. tea contains an infusion of the leaves from the camellia sinensis plant rich in polyphenolic compounds known as catechins, the most abundant of which is (-)-egcg. although tea has been consumed for centuries, it has only recently been studied extensively as a health-promoting beverage that may act to prevent a number of chronic diseases and cancers. the results of several investigations indicate that green tea consumption may b ...200818228206
immunostimulating activity of a crude polysaccharide derived from green tea (camellia sinensis) extract.green tea extract is well-known to reduce the risk of a variety of diseases. here, we investigated the immunostimulating activity of tea polysaccharide (tps), one of the main components in green tea extract. the water extracts from mature or immature tea leaves were precipitated by using ethanol at room temperature. the sediment was washed with ethanol and acetone alternately and then dried. we used the phagocytic activity of macrophage-like cells as an indicator of immune function activation. c ...200818232634
simple and rapid uv spectrophotometry of caffeine in tea coupled with sample pre-treatment using a cartridge column filled with polyvinylpolypyrrolidone (pvpp).we have applied a sample pre-treatment method with a cartridge column filled with polyvinylpolypyrrolidone (pvpp) to the effective removal of polyphenols and simple uv spectrophotometry of caffeine in tea. the absorption maximum length (lambda(max)) for caffeine was close to those for tea catechins in aqueous 1% acetic acid; therefore, the uv spectrum of a non-treated green tea sample had a large absorption wave. in contrast, the absorbance of the green tea sample was gradually reduced by pvpp c ...200818239305
the association between personal habits and bladder cancer in turkey.in this study, we analyzed the effect of turkish coffee and black tea consumption, alcohol intake and smoking on bladder cancer.200818247151
theaflavin-3,3'-digallate, a component of black tea: an inducer of oxidative stress and apoptosis.treatment of human oral squamous carcinoma hsc-2 cells and normal gn46 fibroblasts with theaflavin-3,3'-digallate (tf-3), a polyphenol in black tea, showed a concentration and time dependent inhibition of growth, with the tumor cells more sensitive than the fibroblasts. in buffer and in cell culture medium, tf-3 generated reactive oxygen species, with lower levels detected in buffer amended with catalase and superoxide dismutase, indicating the generation of hydrogen peroxide and superoxide, res ...200818248951
expression and biochemical characterization of beta-primeverosidase and application of beta-primeverosylamidine to affinity purification.beta-primeverosidase (pd) is a family 1 glycosidase catalyzing the hydrolysis of beta-primeverosides (6-o-beta-d-xylopyranosyl-beta-d-glucopyranosides) to release a disaccharide primeverose. to investigate how pd recognizes the disaccharide moiety of beta-primeverosides, the recombinant pd was expressed by a baculovirus-insect cell system. the recombinant pd was secreted from high five cells and was properly modified with n-glycosylation and correct cleavage at the n-terminal signal peptide. the ...200818256510
gas chromatographic method for the determination of hexaconazole residues in black tea.a highly reliable, quantitative and sensitive analytical method for determining the residues of the fungicide, hexaconazole in black tea is described. the proposed method is based on liquid-liquid extraction followed by gas chromatographic determination, using nitrogen phosphorus detector (gc-npd) for the identification and quantitation of hexaconazole. the most appropriate solvent mixture for extracting hexaconazole residues from black tea was n-hexane:acetone at 1:1 (v/v). the extract was clea ...200818257139
immobilized proanthocyanidins for the capture of bacterial lipopolysaccharides.proanthocyanidins (pacs) are an abundant class of compounds found in a variety of plant materials. here we demonstrate the application of these materials as capture molecules for the removal of bacterial lipopolysaccharide (lps) from solution. pacs from whole cranberries, grape juice, black tea, and cranberry juice were purified and immobilized onto thiol-activated sepharose beads. this material was used in pull-down type assays for the capture of lps. the binding of lps by pacs has been shown t ...200818257561
combination chemoprevention of hamster buccal pouch carcinogenesis by bovine milk lactoferrin and black tea polyphenols.combination chemoprevention is a promising approach for oral cancer prevention. the authors evaluated the combined chemopreventive effects of bovine milk lactoferrin (blf) and black tea polyphenols (polyphenon-b) in a clinically relevant in vivo model of 7,12-dimethylbenz[a]anthracene (dmba)-induced hamster buccal pouch (hbp) carcinogenesis. although dietary administration of blf and polyphenon-b alone significantly reduced the tumor incidence, combined administration of blf and polyphenon-b was ...200818259952
association between consumption of black tea and iron status in adult africans in the north west province: the thusa study.the association between black tea consumption and iron status was investigated in a sample of african adults participating in the cross-sectional thusa (transition and health during urbanization of south africans) study in the north west province, south africa. data were analysed from 1605 apparently healthy adults aged 15-65 years by demographic and ffq, anthropometric measurements and biochemical analyses. the main outcome measures were hb and serum ferritin concentrations. no associations wer ...200818275622
l-theanine, a natural constituent in tea, and its effect on mental state.tea is the most widely consumed beverage in the world after water. tea is known to be a rich source of flavonoid antioxidants. however tea also contains a unique amino acid, l-theanine that may modulate aspects of brain function in humans. evidence from human electroencephalograph (eeg) studies show that it has a direct effect on the brain (juneja et al. trends in food science & tech 1999;10;199-204). l-theanine significantly increases activity in the alpha frequency band which indicates that it ...200818296328
methylglyoxal: its presence and potential scavengers.hyperglycemia is the most important factor for the onset and progress of diabetic complications. a growing body of evidence indicates that the increase in reactive carbonyl intermediates such as methylglyoxal (mg) is a consequence of hyperglycemia in diabetes. several studies have shown that higher levels of mg are present in diabetic patients' plasma compared to non-diabetics. glyoxal (go) and mg, the two major alpha-dicarbonyl compounds found in humans, are very reactive and lead to nonenzymat ...200818296351
the influence of different food and drink on tics in tourette syndrome.tourette syndrome (ts) is characterized by waxing and waning motor and vocal tics. because standard medication often remains unsatisfactory, many patients seek alternative medicine. the aim of this study was to increase experience about the influence of food and drinks in ts.200818307557
element composition of tea leaves and tea infusions and its impact on health.tea infusion is the most frequently consumed beverage worldwide next to water, with about 20 billion cups consumed daily. in taiwan, daily consumption averages 2.5 cups of tea infusion per person. many studies have concluded that tea has numerous beneficial effects on health. however, some undesirable trace elements, such as arsenic, chromium, cadmium, lead, etc., are a concern. this study has three aims: (1) to measure the concentrations of arsenic and heavy metal elements, such as chromium, ca ...200818309449
polymer-like polyphenols of black tea and their lipase and amylase inhibitory activities.lipase and amylase inhibitory activities of black tea were examined. after solvent partitioning of a black tea extract with the ethyl acetate and n-butanol, the two soluble fractions showed comparable inhibitory activities. activity in the ethyl acetate fraction was mainly attributable to polyphenols with low-molecular weights, such as theaflavin gallates. on the other hand, the active substance in the n-butanol layer was ascertained to be a polymer-like substance. 1h- and 13c-nmr spectra showed ...200818310934
an exposure and risk assessment for fluoride and trace metals in black tea.exposure and associated health risks for fluoride and trace metals in black tea were estimated. fifty participants were randomly recruited to supply samples from the tea that they drink, and self-administer a questionnaire that inquired about personal characteristics and daily tea intake. analyzed trace metals included aluminum, arsenic, barium, cadmium, cobalt, chromium, copper, manganese, nickel, strontium, and zinc. fluoride and four metals (al, cr, mn, ni) were detected in all samples while ...200818329795
theaflavin-3-gallate and theaflavin-3'-gallate, polyphenols in black tea with prooxidant properties.this study compared the in vitro responses of human gingival fibroblasts and of carcinoma cells derived from the tongue to theaflavin-3-gallate (tf-2a) and theaflavin-3'-gallate (tf-2b), polyphenols in black tea. the antiproliferative and cytotoxic effects of the theaflavin monomers were more pronounced to the carcinoma, than to the normal, cells. in phosphate buffer at ph 7.4, the theaflavins generated hydrogen peroxide and the superoxide anion, suggesting that their mode of toxicity may be due ...200818346048
a case-control study of lifestyle and lung cancer associations by histological types.the objective of the study was to investigate the contribution of dietary factors and physical exercise to the variation in the risk of lung cancer and its major histological types among men and women in the czech republic, and reveal interactions between smoking and diet/physical exercise, if any. in a hospital based case-control study, data collected by in-person interviews from 1096 microscopically confirmed lung cancer cases (587 women, 509 men) and 2966 controls were analyzed using uncondit ...200818348651
coffee, tea, colas, and risk of epithelial ovarian cancer.associations of coffee, tea, and other caffeinated beverages with ovarian cancer risk remain uncertain. in a population-based study in washington state, 781 women with epithelial ovarian cancer diagnosed in 2002 to 2005 and 1,263 controls completed self-administered questionnaires detailing consumption of caffeinated and noncaffeinated coffee, teas, and colas and in-person interviews regarding reproductive and hormonal exposures. we assessed risk associated with coffee, tea, and cola drinking an ...200818349292
polymeric black tea polyphenols induce phase ii enzymes via nrf2 in mouse liver and lungs.among tea polyphenols, the anti-initiating properties of polymeric black tea polyphenols (pbps), the most abundant polyphenols in black tea, are poorly elucidated. hence, this study was undertaken to investigate the effects of pbp extract on the induction of phase ii enzymes. pbp extract induced transcriptional up-regulation of phase ii enzymes in liver and lungs by increasing nrf2-mediated antioxidant-responsive element (are) binding. pbp extract did not alter nrf2 or keap1 at the transcription ...200818358244
characteristics of catechin- and theaflavin-mediated cardioprotection.catechins and theaflavins-the main polyphenolic substances of green and black tea, respectively-exert a plethora of beneficial effects on the cardiovascular system. in a model of h(2)o(2)-mediated oxidative stress, we investigated the effects of epigallocatechin-3-gallate (egcg) and theaflavin-3,3'-digallate (tf3) on neonatal rat cardiomyocytes. pretreatment with egcg or tf3 1 hr prior to induction of oxidative stress by h(2)o(2) effectively protected cardiac myocytes as determined by measuring ...200818367631
levels of selected heavy metals in black tea varieties consumed in saudi arabia.the metal contents (fe, cu, zn, mn, cr, pb, ni, cd, co) of 17 black tea samples were analyzed by using inductively coupled plasma atomic emission spectroscopy (icp-aes). among the investigated metals mn was the highest (1,071.7 microg/g), whereas pb showed minimum levels (0.30 microg/g). iron was the second highest element found in black tea samples. lowest pb concentration (0.30 microg/g) was found in abu jabal tea; whereas maximum (2.2 microg/g) was found in manasul tea. lowest cd concentratio ...200818373271
a mixture of extracts of black and green teas and mulberry leaf did not reduce weight gain in rats fed a high-fat diet.tea extracts are used in many over-the-counter preparations claiming to promote weight loss. the rationale for this usage includes reports that green tea extract increases thermogenesis, and extracts of green and black tea and mulberry leaf inhibit the digestion/absorption of carbohydrate and fat. the investigators in this study tested the potential of increasing doses of a mixture of three extracts (50-percent black tea, 20-percent green tea, and 30-percent mulberry) to induce weight loss, stea ...200818377102
nutritional risk factors for the development of hypertension in diabetic patients.the aim of this cross-sectional study was to determine the significant limitations, sensitivity, specificity, partial correlations, and odds ratios of nutrient intake in patients with and without hypertension with type 2 diabetes mellitus. diabetic patients (n=220) with clinical diagnosis of hypertension and diabetic patients (n=230) without hypertension were included in this study. the questionnaire form included a list of 65 food items formed from five main food groups (grain, meat and alterna ...200918394930
polymeric black tea polyphenols inhibit mouse skin chemical carcinogenesis by decreasing cell proliferation.the aim of this study was to investigate the antitumour promoting effects and possible mechanisms of action of the most abundant polymeric black tea polyphenols (pbps 1-5) or thearubigins, in vivo.200818400024
evaluation of toxicity of green tea catechins with 90-day dietary administration to f344 rats.green tea catechins (gtc), polyphenols extracted from the stalks and leaves of camellia sinensis, are found in the different types of tea beverages and as antioxidant additives to many foods, snacks, fats and fatty oils. as a part of their safety assessment, subchronic toxicity was investigated in male and female f344 rats with dietary administration at concentrations of 0 (control), 0.3%, 1.25% and 5.0% for 90 days. the average daily intakes of gtc in each group were 180, 764 and 3525mg/kg body ...200818400353
iron absorption in young indian women: the interaction of iron status with the influence of tea and ascorbic acid.ascorbic acid (aa) enhances and tea inhibits iron absorption. it is unclear whether iron status influences the magnitude of this effect.200818400710
potential anthelmintics: polyphenols from the tea plant camellia sinensis l. are lethally toxic to caenorhabditis elegans.a novel gallate of tannin, (-)-epigallocatechin-(2 beta-->o-->7',4 beta-->8')-epicatechin-3'-o-gallate (8), together with (-)-epicatechin-3-o-gallate (4), (-)-epigallocatechin (5), (-)-epigallocatechin-3-o-gallate (6), and (+)-gallocatechin-(4 alpha-->8')-epigallocatechin (7), were isolated from the tea plant camellia sinensis (l.) o. kuntze var. sinensis (cv., yabukita). the structure of 8, including stereochemistry, was elucidated by spectroscopic methods and hydrolysis. the compounds, along w ...200818404315
preparative isolation and purification of theaflavins and catechins by high-speed countercurrent chromatography.high-speed countercurrent chromatography (hsccc) has been applied for the separation of theaflavins and catechins. the hsccc run was carried out with a two-phase solvent system composed of hexane-ethyl acetate-methanol-water-acetic acid (1:5:1:5:0.25, v/v) by eluting the lower aqueous phase at 2 ml/min at 700 rpm. the results indicated that pure theaflavin, theaflavins-3-gallate, theaflavins-3'-gallate and theaflavin-3,3'-digallate could be obtained from crude theaflavins sample and black tea. t ...200818436487
quality assessment and quantitative analysis of flavonoids from tea samples of different origins by hplc-dad-esi-ms.components of green tea ( camellia sinensis) have been of considerable interest in recent years because of their potential utility as pharmaceutical agents, particularly for their antioxidant and anticarcinogenic activity. responding to the increasing scientific validation of numerous health benefits of tea, a comprehensive approach was adopted to carry out analysis for the quality assessment of flavonoids in tea samples of different origins. for this purpose, extraction, separation, and mass sp ...200818442252
pharmacological studies on indian black tea (leaf variety) in acute and chronic inflammatory conditions.infusions of indian black tea (bti), when administered orally, produced significant inhibition of rat paw oedema, induced with carrageenin (pre and post treatment) and arachidonic acid. bti was also found to inhibit peritoneal capillary permeability and caused a marked reduction of lipopolysaccharide induced pge(2) generation. in these models, the observed antioedema effect was similar to that of bw755c (a dual inhibitor of cyclooxygenase and 5-lipoxygenase enzymes). bti was found to scavenge su ...200818446842
contribution of p53-mediated bax transactivation in theaflavin-induced mammary epithelial carcinoma cell apoptosis.theaflavins, the bioactive flavonoids of black tea, have been demonstrated to inhibit proliferation and induce apoptosis in a variety of cancer cells. however, the contribution of p53 in mammary epithelial carcinoma cell apoptosis by theaflavins remains unclear. it has been reported that p53 triggers apoptosis by inducing mitochondrial outer membrane permeabilization through transcription-dependent and -independent mechanisms. using wild-type and mutant p53-expressing as well as p53-null cells w ...200818454316
cloning and differential expression of qm like protein homologue from tea [camellia sinensis (l.) o. kuntze].the qm like protein gene encodes for ribosomal protein l10, which is implicated in tumor suppression, transcription factor regulation, and ribosome stability in yeast and mammals. present study describes cloning of a full-length qm cdna (csqm) from tea leaves using differential display of mrna followed by rapid amplification of cdna ends. expression of csqm was studied in leaves of different stages of development and under various external cues. csqm contained an open reading frame of 651 bases, ...200918454353
association between soy and green tea (camellia sinensis) diminishes hypercholesterolemia and increases total plasma antioxidant potential in dyslipidemic subjects.to evaluate the hypolipemic and antioxidant effects of soy and green tea alone and/or in association in dyslipidemic subjects.200818455656
different types of tea products attenuate inflammation induced in trypanosoma brucei infected mice.an in vivo study was carried out to determine the effect of different types of kenyan tea extracts on male swiss albino mice infected with trypanosoma brucei brucei isolate ketri 2710. the isolate produced a similar clinical picture after a pre-patent period of 5 days post-infection (dpi). parasitemia levels in the untreated mice and those given different teas developed exponentially at similar rates reaching similar densities at the peak of parasitemia 8 dpi. between 9 and 13 dpi parasitemia de ...200818456544
shoot epicatechin and epigallocatechin contents respond to water stress in tea [camellia sinensis (l.) o. kuntze].an experiment was conducted to determine the association of tea catechins to water stress in tea, with the objective of determining their suitability as indicators for predicting drought tolerance in tea (camellia sinensis). the study consisted of six tea clones (bbk 35, trfk 6/8, trfk 76/1, trfk 395/2, trfk 31/30, and trfk 311/287) and four levels of soil water content (38, 30, 22, and 14% v/v), which were arranged in a complete randomized design and replicated 3 times. the treatments were main ...200818460799
safety of green tea extracts : a systematic review by the us pharmacopeia.green tea [camellia sinensis (l.) kuntze] is the fourth most commonly used dietary supplement in the us. recently, regulatory agencies in france and spain suspended market authorization of a weight-loss product containing green tea extract because of hepatotoxicity concerns. this was followed by publication of adverse event case reports involving green tea products. in response, the us pharmacopeia (usp) dietary supplement information expert committee (dsi ec) systematically reviewed the safety ...200818484782
epigallocatechin gallate improves serum lipid profile and erythrocyte and cardiac tissue antioxidant parameters in wistar rats fed an atherogenic diet.oxidative stress is believed to contribute to the pathogenesis of hypercholesterolaemic atherosclerosis; hence, various antioxidant compounds are being evaluated for potential anti-atherogenic effects. the present study assessed the efficacy of epigallocatechin gallate (egcg), an antioxidant component of the plant camellia sinensis, in improving serum lipid profile and antioxidant parameters in erythrocytes and cardiac tissue in rats fed an atherogenic diet. in male albino wistar rats fed an ath ...200818485145
evaluation of black tea gel and its protection potential against uv.in this study, aqueous and alcoholic extracts of black tea were obtained. the black tea extracts were tested in vitro for their ultraviolet (uv) absorption profile. it was found that both extracts showed uv absorption and followed the same path based on the wavelength. aqueous extract showed a stronger absorptivity per weight basis than the alcoholic extract of black tea. a peak was obtained between 250 and 300 nm. after 300 nm, uv absorption decreased fast towards 400 nm with a low absorptivity ...200718489382
structural determination and dpph radical-scavenging activity of two acylated flavonoid tetraglycosides in oolong tea (camellia sinensis).two major acylated flavonoid tetraglycosides were isolated from the methanol extract of oolong tea. their structures were elucidated by spectroscopic methods as quercetin 3-o-[2(g)-(e)-coumaroyl-3(g)-o-beta-d-glucosyl-3(r)-o-beta-d-glucosylrutinoside] (1) and kaempferol 3-o-[2(g)-(e)-coumaroyl-3(g)-o-beta-d-glucosyl-3(r)-o-beta-d-glucosylrutinoside] (2). compounds 1 and 2 exhibited scavenging activity against dpph radical with ec(50) values of 30.5 and 487.2 microm, respectively.200818520094
the acute effect of green tea consumption on endothelial function in healthy individuals.tea consumption is associated with decreased cardiovascular risk. flow-mediated dilatation (fmd) of the brachial artery is related to coronary endothelial function and it is an independent predictor of cardiovascular risk. black tea has a beneficial effect on endothelial function; the effect, however, of green tea on brachial artery reactivity has not been defined yet.200818525384
expression of caffeine biosynthesis genes in tea (camellia sinensis).using semi-quantitative reverse transcription-pcr, we studied the expression of genes encoding caffeine synthase (tcs1), inosine-5'-monophosphate dehydrogenase (tidh), s-adenosyl-l-methionine synthase (sams), phenylalanine ammonia-lyase (pal) and alpha-tubulin (tua1) in young and mature leaves, stems and roots of 4-month-old tea seedlings and young and old tea tissue cultures. the amounts of transcripts of tcs1 were much higher in young leaves than in other parts of the plant. expression of tidh ...201318533472
pretreatment with black tea polyphenols modulates xenobiotic-metabolizing enzymes in an experimental oral carcinogenesis model.the objective of this study was to evaluate the chemopreventive potential of the black tea polyphenols polyphenon-b and btf-35 during the preinitiation phase of 7,12-dimethylbenz[a]anthracene (dmba)-induced hamster buccal pouch (hbp) carcinogenesis. hamsters were divided into six groups. animals in groups 2 and 3 received diet containing polyphenon-b and btf-35, respectively, 4 weeks before carcinogen administration when they were 6 weeks of age and continued until the final exposure to carcinog ...200818543609
influence of green and black tea on folic acid pharmacokinetics in healthy volunteers: potential risk of diminished folic acid bioavailability.previous in vitro studies using caco-2 cell monolayers suggested a possible interaction between green and black tea and folic acid at the level of intestinal absorption. the main purpose of the present study was to investigate a possible pharmacokinetic interaction between tea and folic acid in healthy volunteers. in an open-labeled randomized cross-over study, the pharmacokinetic interaction between tea and folic acid (0.4 mg and 5 mg) was investigated in healthy volunteers. water was used as t ...200818551467
effect of black tea brew of camellia sinensis on sexual competence of male rats.in sri lankan traditional medicine black tea brew (btb) of camellia sinensis (l.) o. kuntze (theaceae) is claimed to have male sexual stimulant activity. as this claim is not scientifically tested and proven, this study was undertaken to evaluate the effects of btb on male sexual competence.200818565706
extract of black tea (pu-ehr) inhibits postprandial rise in serum cholesterol in mice, and with long term use reduces serum cholesterol and low density lipoprotein levels and renal fat weight in rats.a water-soluble extract of a traditional chinese fermented black tea, pu-ehr, decomposes bile acid cholesterol micelles. this black tea extract (bte) was studied to see if it could decrease the postprandial elevation of blood cholesterol levels after a single administration in ddy mice. it was found that bte (0.3 g/kg) significantly decreased the postprandial rise in blood cholesterol levels after oral administration of cholesterol (130 mg/kg). a non-fermented tea (i.e. green tea) extract did no ...200818570239
[toxic hepatitis due to camellia sinensis]. 201618570823
a physiological model of tea-induced astringency.the mechanism by which solutions containing polyphenols are perceived as astringent is not clearly understood. salivary proline-rich proteins and histatins are products of salivary glands and rapidly bind polyphenols - thought to be the main astringent compound in such as tea and wine. however it is unclear how this interaction leads to the altered oral mouthfeel known as astringency which is characterised by a dry, puckered feeling all around the mouth. to determine the role of saliva in the pe ...200818590751
an early gene of the flavonoid pathway, flavanone 3-hydroxylase, exhibits a positive relationship with the concentration of catechins in tea (camellia sinensis).tea (camellia sinensis (l.) o. kuntze) leaves are a major source of flavonoids that mainly belong to the flavan 3-ols or catechins. apart from being responsible for tea quality, these compounds have medicinal properties. flavanone 3-hydroxylase (f3h) is an abundant enzyme in tea leaves that catalyzes the stereospecific hydroxylation of (2s)-naringenin to form (2r,3r)-dihydrokaempferol. we report a full-length cdna sequence of f3h from tea (csf3h accession no. ay641730). csf3h comprised 1365 bp w ...200818595847
flavonoids, flavonoid-rich foods, and cardiovascular risk: a meta-analysis of randomized controlled trials.the beneficial effects of flavonoid consumption on cardiovascular risk are supported by mechanistic and epidemiologic evidence.200818614722
a preconcentration system for determination of copper and nickel in water and food samples employing flame atomic absorption spectrometry.a separation/preconcentration procedure using solid phase extraction has been proposed for the flame atomic absorption spectrometric determination of copper and nickel at trace level in food samples. the solid phase is dowex optipore sd-2 resin contained on a minicolumn, where analyte ions are sorbed as 5-methyl-4-(2-thiazolylazo) resorcinol chelates. after elution using 1 mol l(-1) nitric acid solution, the analytes are determinate employing flame atomic absorption spectrometry. the optimizatio ...200918620809
in vitro assessment of the effectiveness of whitening dentifrices for the removal of extrinsic tooth stains.this in vitro study evaluated the effectiveness of whitening dentifrices for the removal of extrinsic tooth stains. twenty dental blocks (4 x 4 mm), including enamel and dentine, removed from freshly extracted bovine incisors, were randomly divided into 4 groups: g1--distilled water, g2--colgate, g3--crest extra whitening and g4--rapid white. in all specimens, the dentin was covered with colorless nail polish, and the enamel was left exposed. next, the specimens were immersed in a solution of bl ...200818622478
nongallated compared with gallated flavan-3-ols in green and black tea are more bioavailable.green tea and black tea (bt) contain gallated [(-)-epigallocatechin-3-gallate (egcg), (-)-epicatechin-3-gallate] and nongallated [(-)-epicatechin, (-)-epigallocatechin (egc)] tea polyphenols (pp). during bt production, pp undergo oxidation and form larger polymers such as theaflavins (the) and thearubigins, which contribute to the health benefit of bt. this article gives an overview of the role of chemical characteristics and endogenous metabolism of tea pp and their bioavailability in humans an ...200818641202
a review of the epidemiological evidence on tea, flavonoids, and lung cancer.tea and its main bioactive ingredients, the flavonoids, have been associated with human cancer for several decades. in this article, an overview is provided of observational epidemiological studies of lung cancer incidence in relation to intake of green tea, black tea, flavonols/flavones, and catechins. a pubmed search was conducted in september 2007. articles were selected if they provided risk ratios (relative risk or odds ratio) for lung cancer and were of observational design (cohort, case-c ...200818641207
green tea consumption is associated with decreased dna damage among gstm1-positive smokers regardless of their hogg1 genotype.the levels of tobacco-related dna adducts in human tissues reflect a dynamic process that is dependent on the intensity and time of exposure to tobacco smoke, the metabolic balance between activation of detoxification mechanisms, and the removal of adducts by dna repair and/or cell turnover. urinary 8-hydroxydeoxyguanosine (8-ohdg) is probably 1 of the most abundant dna lesions formed during oxidative stress and is proposed as a sensitive biomarker of the overall oxidative dna damage and repair. ...200818641208
effect of black tea in diethylnitrosamine-induced esophageal carcinogenesis in mice.to evaluate the effect of black tea on esophageal carcinogenesis induced by the oral administration of diethylnitrosamine (den).200818641802
[mechanism study of tea pigment on the selective against the colony growth of preneoplastic and normal syrian hamster embryo cells].to use co-cultures of she normal and preneoplastic cells in order to study the chemopreventive effects of doses of tea pigment (tp) from black tea on growth, proliferation, apoptosis and related regulation gene expression of normal and preneoplastic she cells.200818646525
beverage consumption and the prevalence of tooth loss in pregnant japanese women: the osaka maternal and child health study.recently, there has been growing interest in the impact of beverage consumption on dental health because changes have occurred in the types and quantities of beverage consumed. this cross-sectional study investigated the relationship between consumption frequencies of various beverages and the prevalence of tooth loss among young adult women. study subjects were 1002 pregnant japanese women. tooth loss was defined as previous extraction of one or more teeth. dietary habits were evaluated by a va ...200818646593
determination of theanine, gaba, and other amino acids in green, oolong, black, and pu-erh teas with dabsylation and high-performance liquid chromatography.dabsyl chloride (dimethylaminoazobenzene sulfonyl chloride), a useful chromophoric labeling reagent for amino acids and amines, was developed in this laboratory in 1975. although several methods have been developed to determine various types of amino acids, a quick and easy method of determining theanine, gaba, and other amino acids has not been developed in one hplc system. in this paper are analyzed the free amino acid contents of theanine and gaba in different teas (green tea, black tea, oolo ...200818652476
cloning of two cdnas encoding a family of atp sulfurylase from camellia sinensis related to selenium or sulfur metabolism and functional expression in escherichia coli.atp sulfurylase, the first enzyme in the sulfate assimilation pathway of plants, catalyzes the formation of adenosine phosphosulfate from atp and sulfate. here we report the cloning of two cdnas encoding atp sulfurylase (aps1 and aps2) from camellia sinensis. they were isolated by rt-pcr and race-pcr reactions. the expression of aps1 and aps2 are correlated with the presence of atp sulfurylase enzyme activity in cell extracts. aps1 is a 1415-bp cdna with an open reading frame predicted to encode ...200818657428
[chemical studies on plant polyphenols and formation of black tea polyphenols].recent biological and pharmacological studies strongly suggested that plant polyphenols in foods, beverages and crude drugs have various health benefits. however, still there are chemically uncharacterized polyphenols, especially those with large molecular weights. the typical example is black tea polyphenols. four tea catechins of fresh tea leaves are enzymatically oxidized in tea fermentation process of black tea manufacture to give a complex mixture of the oxidation products. despite many eff ...200818670177
proteome analysis of tea pollen (camellia sinensis) under different storage conditions.the protein complement of tea pollen collecting from tea tree (camellia sinensis) was compared under different storage conditions. protein was partially identified using a combination of the 2d-page, maldi-tof/ms, mascot, and xproteo search engine. two hundred and sixty-nine and 396 proteins were detected in pollen stored at room temperature (rt) and -20 degrees c, respectively. forty-three of the identified proteins were assigned to defense-related functions, energy metabolism, cytoskeleton, nu ...200818680303
topographic and radiographic profile assessment of dental erosion. part iii: effect of green and black tea on human dentition.this study compared green and black tea to soda and orange juice in terms of their erosive effect on the human dentition. vinegar and water were used as active and passive control fluids. an accelerated in vitro test was used to monitor how short- and long-term exposure to these fluids affected the topography and morphology of the coronal segments of the human dentition. this 20-week test was conducted under controlled conditions, independent of the influencing factors of the oral environment. t ...200818683403
effect of genistein on the bioavailability and intestinal cancer chemopreventive activity of (-)-epigallocatechin-3-gallate.the green tea (camellia sinensis) catechin, (-)-epigallocatechin-3-gallate (egcg), has shown cancer-preventive activity in animal models. previously, we have reported the bioavailability of egcg in rats and mice. here, we report that cotreatment of ht-29 human colon cancer cells with genistein (from soy) increased cytosolic egcg by 2- to 5-fold compared with treatment with egcg only. inclusion of genistein, at non-cytotoxic concentrations, increased the growth inhibitory effects of egcg against ...200818684728
the effects of sour tea (hibiscus sabdariffa) on hypertension in patients with type ii diabetes.to compare the antihypertensive effectiveness of sour tea (st; hibiscus sabdariffa) with black tea (bt) infusion in diabetic patients, this double-blind randomized controlled trial was carried out. sixty diabetic patients with mild hypertension, without taking antihypertensive or antihyperlipidaemic medicines, were recruited in the study. the patients were randomly allocated to the st and bt groups and instructed to drink st and bt infusions two times a day for 1 month. their blood pressure (bp) ...200918685605
selective effects of tea extract and its phenolic compounds on human peripheral blood mononuclear cell cytokine secretions.the effects of black tea extract (bte) and some of its pure phenolics on human peripheral blood mononuclear cell (pbmc) cytokine secretion were examined in vitro.200918686109
new phenolic components and chromatographic profiles of green and fermented teas.a standardized profiling method based on liquid chromatography with diode array and electrospray ionization mass spectrometric detection (lc-dad-esi/ms) was applied to establish the phenolic profiles of 41 green teas and 25 fermented teas. more than 96 phenolic compounds were identified that allowed the teas to be organized into five groups. epigallocatechin gallate (egcg) was the major phenolic component of green tea made from mature leaves (group 2), while green tea made from the younger buds ...200818686968
characterization of cellulose production by a gluconacetobacter xylinus strain from kombucha.the aims of this work were to characterize and improve cellulose production by a gluconoacetobacter xylinus strain isolated from kombucha and determine the purity and some structural features of the cellulose from this strain. cellulose yield in tea medium with both black tea and green tea and in hestrin and schramm (hs) medium under both static and agitated cultures was compared. in the tea medium, the highest cellulose yield was obtained with green tea (approximately 0.20 g/l) rather than blac ...200818704575
a population-based, case-control study of green tea consumption and leukemia risk in southwestern taiwan.this study investigated the association between green tea consumption and leukemia.200918752033
medicinal flowers. xxii. structures of chakasaponins v and vi, chakanoside i, and chakaflavonoside a from flower buds of chinese tea plant (camellia sinensis).two acylated oleanane-type triterpene oligoglycosides, chakasaponins v and vi, an aromatic glycoside, chakanoside i, and an acylated flavonol oligoglycoside, chakaflavonoside a, were isolated from the flower buds of chinese tea plant [camellia sinensis (l.) o. kuntze]. the chemical structures of those new glycosides were elucidated on the basis of chemical and physicochemical evidence.200818758105
efficacy and safety of chinese black tea (pu-ehr) extract in healthy and hypercholesterolemic subjects.water-extracted chinese black tea (pu-ehr) exerts a precipitating effect on mixed bile salt micelles in foods. the amount of black tea extract (bte) effective for hypercholesterolemia (hc) was examined in humans.200818769024
total polyphenol content and antioxidant capacity of commercially available tea (camellia sinensis) in argentina.tea, camellia sinensis (l.) o. kuntze (theaceae) is cultivated in argentina in the northeastern region (provinces of misiones and corrientes), between 26 degrees and 28 degrees south latitude, the southernmost area of the world where tea is cultivated. the objective of this work was to determine the total polyphenol content and the in vitro antioxidant capacity of green and black tea cultivated and industrialized in argentina. twelve samples of eight brands were analyzed. the total polyphenol co ...200818778031
antibacterial effects of green tea polyphenols on clinical isolates of methicillin-resistant staphylococcus aureus.the antibacterial effects of tea polyphenols (tpp) extracted from korean green tea (camellia sinensis) against clinical isolates of methicillin-resistant staphylococcus aureus (mrsa) were evaluated. characterization of the minimal inhibitory concentration (mic) of oxacillin for 30 s. aureus strains isolated from patients treated with oxacillin identified 13 strains with an oxacillin mic >or= 4 microg/ml as methicillin-resistant staphylococcus aureus (mrsa) (range: 8 to 512 microg/ml), while 17 s ...200818781360
preservation of kombucha tea-effect of temperature on tea components and free radical scavenging properties.kombucha tea is sugared black tea fermented with a consortium of acetic acid bacteria and yeasts (tea fungus) for 14 days. the tea tastes slightly sweet and acidic. the formation of tea fungal biofilms during storage is a big problem when kombucha tea is being stored and commercialized. various thermal treatments have been tried for long-term storage of kombucha tea. the present study revealed the influence of heat on the biochemical constituents and the free radical scavenging properties of kom ...200818781766
high level of genetic diversity among the selected accessions of tea (camellia sinensis) from abandoned tea gardens in western himalaya.to revive cultivation of the tea unique to the western himalayan region, it is important to evaluate the seed-derived bushes available in the area's abandoned gardens. this study used quantitative leaf characters, catechin content, and aflp markers to assess these china cultivar type bushes. compared with other china cultivar germplasm, these accessions showed a higher level of diversity among themselves. among the quantitative morphological characters, leaf length is important in distinguishing ...200818784998
characterisation of lysozyme activity in the in situ pellicle using a fluorimetric assay.lysozyme is among the most protective enzymes in the pellicle layer. the aim of the present study was to establish a precise fluorimetric assay for determination and characterisation of lysozyme activity immobilised in the initial in situ formed pellicle. for in situ pellicle formation, bovine enamel slabs were fixed on maxillary splints and carried by six subjects for different times (3, 30 min) on buccal and palatal sites. the enzymatic assay was based on hydrolysis of cell walls from micrococ ...200918810509
tea polyphenols benefit vascular function.tea, the most popular beverage worldwide, is consumed in three basic forms; green tea, black tea and oolong tea. tea contains over 4,000 chemicals some of which are bioactive. in recent years there has been a mounting interest in understanding the cardiovascular and metabolic benefits of polyphenolic flavonoids in tea, which can be used as a supplement among patients. diverse cardioprotective effects of consuming tea or tea polyphenols have been described on pathological conditions, e. g. hypert ...200818815738
quantitative assessment of metals in local brands of tea in pakistan.in present study, mn, fe, zn, cu, co, pb, cr, ni and cd were analyzed by faas in green and black tea samples of locally available in the pakistani market. na and k were also determined by flame photometer. tea leaves can be the source of mineral components and trace elements, as well as some undesirable substances due to exposure to the environment. among the metals tested, k was the most abundant one followed by na, mn and fe. fortunately, toxic heavy metals, pb and cd, had the lowest contents ...200818817205
volatile components of camellia sinensis inhibit growth and biofilm formation of oral streptococci in vitro.this study aimed to evaluate the efficacy of semi fermented and non fermented camellia sinensis extracts (black and green tea) and comparison between them against streptococcus mutans atcc 25175, s. mitis atcc 9811 and s. sanguis atcc 10556 that are responsible for dental caries and bacteremias following dental manipulations. minimum inhibitory concentrations of both tea extracts were assessed by well diffusion and broth dilution methods and examination of cell adherence (biofilm inhibitory conc ...200818817265
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