Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| composition by lc-ms/ms of new carotenoid esters in mango and citrus. | interest in the composition of carotenoid esters of fruits is growing because esterification may affect their bioavailability. thus, the aim was to provide a detailed identification of carotenoid esters in citrus and mango. orange cv. 'valencia' and cv. 'pera' presented 9 free carotenoids, 38 monoesters, and 60 diesters. violaxanthin and luteoxanthin derivatives were the major ones, followed by antheraxanthin, lutein, zeaxanthin, β-cryptoxanthin, and zeinoxanthin esters, many of them reported fo ... | 2016 | 27712060 |
| led blue light-induced changes in phenolics and ethylene in citrus fruit: implication in elicited resistance against penicillium digitatum infection. | the objective was to investigate whether led blue light (lbl) induces changes in phenolics and ethylene production of sweet oranges, and whether they participate in lbl-elicited resistance against the most important postharvest pathogen (penicillium digitatum) of citrus fruit. the expression of relevant genes of the phenylpropanoid and ethylene biosynthetic pathways during elicitation of resistance was also determined. different lbl (wavelength 450nm) quantum fluxes were used within the 60-630μm ... | 0 | 27719952 |
| the diversity of citrus endophytic bacteria and their interactions with xylella fastidiosa and host plants. | the bacterium xylella fastidiosa is the causal agent of citrus variegated chlorosis (cvc) and has been associated with important losses in commercial orchards of all sweet orange [citrus sinensis (l.)] cultivars. the development of this disease depends on the environmental conditions, including the endophytic microbial community associated with the host plant. previous studies have shown that x. fastidiosa interacts with the endophytic community in xylem vessels as well as in the insect vector, ... | 2016 | 27727362 |
| protective effects of sweet orange, unshiu mikan, and mini tomato juice powders on t-bhp-induced oxidative stress in hepg2 cells. | the aim of this study was to investigate the protective effects of juice powders from sweet orange [citrus sinensis (l.) osbeck], unshiu mikan (citrus unshiu marcow), and mini tomato (solanum lycopersicum l.), and their major flavonoids, hesperidin, narirutin, and rutin in tert-butyl hydroperoxide (t-bhp)-induced oxidative stress in hepg2 cells. the increased reactive oxygen species and decreased glutathione levels observed in t-bhp-treated hepg2 cells were ameliorated by pretreatment with juice ... | 2016 | 27752497 |
| metabolomic comparative analysis of the phloem sap of curry leaf tree (bergera koenegii), orange jasmine (murraya paniculata), and valencia sweet orange (citrus sinensis) supports their differential responses to huanglongbing. | orange jasmine, murraya paniculata and curry leaf tree, bergera koenegii are alternative hosts for diaphorina citri, the vector of candidatus liberibacter asiaticus (clas), the pathogen of huanglongbing (hlb) in citrus. d. citri feeds on the phloem sap where clas grows. it has been shown that orange jasmine was a better host than curry leaf tree to d. citri. in addition, clas can infect orange jasmine but not curry leaf tree. here, we compared the phloem sap composition of these 2 plants to the ... | 2016 | 27763819 |
| population structure and diversity of citrus tristeza virus (ctv) isolates in hunan province, china. | stem-pitting (sp) is the main type of citrus tristeza virus (ctv) that causes severe damage to citrus trees, especially those of sweet orange, in hunan province, china. understanding the local ctv population structure should provide clues for effective mild strain cross-protection (mscp) of the sp strain of ctv. in this study, markers for the p23 gene, multiple molecular markers (mmms), and sequence analysis of the three silencing suppressor genes (p20, p23 and p25) were employed to analyze the ... | 2017 | 27771790 |
| metabolomic analysis of primary metabolites in citrus leaf during defense responses. | mechanical damage is one of the unavoidable environmental stresses to plant growth and development. plants induce a variety of reactions which defend against natural enemies and/or heal the wounded sites. jasmonic acid (ja) and salicylic acid (sa), defense-related plant hormones, are well known to be involved in induction of defense reactions and play important roles as signal molecules. however, defense related metabolites are so numerous and diverse that roles of individual compounds are still ... | 2017 | 27789172 |
| ars-media for excel: a spreadsheet tool for calculating media recipes based on ion-specific constraints. | ars-media for excel is an ion solution calculator that uses "microsoft excel" to generate recipes of salts for complex ion mixtures specified by the user. generating salt combinations (recipes) that result in pre-specified target ion values is a linear programming problem. excel's solver add-on solves the linear programming equation to generate a recipe. calculating a mixture of salts to generate exact solutions of complex ionic mixtures is required for at least 2 types of problems- 1) formulati ... | 2016 | 27812202 |
| genome wide selection in citrus breeding. | genome wide selection (gws) is essential for the genetic improvement of perennial species such as citrus because of its ability to increase gain per unit time and to enable the efficient selection of characteristics with low heritability. this study assessed gws efficiency in a population of citrus and compared it with selection based on phenotypic data. a total of 180 individual trees from a cross between pera sweet orange (citrus sinensis osbeck) and murcott tangor (citrus sinensis osbeck x ci ... | 2016 | 27813590 |
| draft genome sequence of curtobacterium sp. strain er1/6, an endophytic strain isolated from citrus sinensis with potential to be used as a biocontrol agent. | herein, we report a draft genome sequence of the endophytic curtobacterium sp. strain er1/6, isolated from a surface-sterilized citrus sinensis branch, and it presented the capability to control phytopathogens. functional annotation of the ~3.4-mb genome revealed 3,100 protein-coding genes, with many products related to known ecological and biotechnological aspects of this bacterium. | 2016 | 27856581 |
| vascular epiphytes and host trees of ant-gardens in an anthropic landscape in southeastern mexico. | ant-gardens (ags) are considered one of the most complex mutualist systems between ants and plants, since interactions involving dispersal, protection, and nutrition occur simultaneously in them; however, little is known about the effects of the transformation of ecosystems on their diversity and interactions. in five environments with different land use within an anthropic landscape in southeastern mexico, we investigated the diversity and composition of epiphytes and host trees of ags built by ... | 2016 | 27866229 |
| transgenic citrus expressing synthesized cecropin b genes in the phloem exhibits decreased susceptibility to huanglongbing. | expression of synthesized cecropin b genes in the citrus phloem, where candidatus liberibacter asiaticus resides, significantly decreased host susceptibility to huanglongbing. huanglongbing (hlb), associated with candidatus liberibacter asiaticus bacteria, is the most destructive disease of citrus worldwide. all of the commercial sweet orange cultivars lack resistance to this disease. the cationic lytic peptide cecropin b, isolated from the chinese tasar moth (antheraea pernyi), has been shown t ... | 2017 | 27866312 |
| citrus psorosis virus coat protein-derived hairpin construct confers stable transgenic resistance in citrus against psorosis a and b syndromes. | citrus psorosis virus (cpsv) is the causal agent of psorosis, a serious and widespread citrus disease. two syndromes of psorosis, psa and psb, have been described. psb is the most aggressive and rampant form. previously, we obtained pineapple sweet orange plants transformed with a hairpin construct derived from the cpsv coat protein gene (ihpcp). some of these plants were resistant to cpsv 90-1-1, a psa isolate homologous to the transgene. in this study, we found that expression of the ihpcp tra ... | 2017 | 27891561 |
| hybrid origins of citrus varieties inferred from dna marker analysis of nuclear and organelle genomes. | most indigenous citrus varieties are assumed to be natural hybrids, but their parentage has so far been determined in only a few cases because of their wide genetic diversity and the low transferability of dna markers. here we infer the parentage of indigenous citrus varieties using simple sequence repeat and indel markers developed from various citrus genome sequence resources. parentage tests with 122 known hybrids using the selected dna markers certify their transferability among those hybrid ... | 2016 | 27902727 |
| citrus leprosis virus c infection results in hypersensitive-like response, suppression of the ja/et plant defense pathway and promotion of the colonization of its mite vector. | leprosis is a serious disease of citrus caused by citrus leprosis virus c (cilv-c, genus cilevirus) whose transmission is mediated by false spider mites of the genus brevipalpus. cilv-c infection does not systemically spread in any of its known host plants, thus remaining restricted to local lesions around the feeding sites of viruliferous mites. to get insight into this unusual pathosystem, we evaluated the expression profiles of genes involved in defense mechanisms of arabidopsis thaliana and ... | 2016 | 27933078 |
| genome-wide identification and functional analysis of s-rnase involved in the self-incompatibility of citrus. | s-rnase-based self-incompatibility is found in solanaceae, rosaceae, and scrophulariaceae, and is the most widespread mechanism that prevents self-fertilization in plants. although 'shatian' pummelo (citrus grandis), a traditional cultivated variety, possesses the self-incompatible trait, the role of s-rnases in the self-incompatibility of 'shatian' pummelo is poorly understood. to identify genes associated with self-incompatibility in citrus, we identified 16 genes encoding homologs of ribonucl ... | 2017 | 27933381 |
| analysis of whole chloroplast genomes from the genera of the clauseneae, the curry tribe (rutaceae, citrus family). | the clauseneae (aurantioideae, rutaceae) is a tribe in the citrus family that, although economically important as it contains the culinary and medicinally-useful curry tree (bergera koenigii), has been relatively understudied. due to the recent significant taxonomic changes made to this tribe, a closer inspection of the genetic relationships among its genera has been warranted. whole genome skimming was used to generate chloroplast genomes from six species, representing each of the four genera ( ... | 2016 | 27965082 |
| assessing a traceability technique in fresh oranges (citrus sinensis l. osbeck) with an hs-spme-gc-ms method. towards a volatile characterisation of organic oranges. | a targeted approach using hs-spme-gc-ms was performed to compare flavour compounds of 'navelina' and 'salustiana' orange cultivars from organic and conventional management systems. both varieties of conventional oranges showed higher content of ester compounds. on the other hand, higher content of some compounds related with the geranyl-diphosphate pathway (neryl and geranyl acetates) and some terpenoids were found in the organic samples. furthermore, the partial least square discriminant analys ... | 2017 | 27979182 |
| correlation between classification in risk categories and clinical aspects and outcomes. | to correlate classification in risk categories with the clinical profiles, outcomes and origins of patients. | 2016 | 27982310 |
| survival strategies of citrus rootstocks subjected to drought. | two citrus rootstocks, rangpur lime (rl) and sunki maravilha mandarin (sm), were analyzed either ungrafted or grafted with their reciprocal graft combinations or with shoot scions of two commercial citrus varieties: valencia orange (vo) and tahiti acid lime (tal). all graft combinations were subjected to distinct watering regimes: well-watered, severe drought and rehydration. growth and water relation parameters, gas exchange as well as sugar and hormone profiles were determined. data indicated ... | 2016 | 27996018 |
| effect of postharvest practices including degreening on citrus carpoplane microbial biomes. | to investigate the effect of commercial citrus packhouse processing steps on the fruit surface microbiome of clementines and palmer navel oranges. | 2017 | 28052466 |
| microrna annotation of plant genomes - do it right or not at all. | micrornas are non-coding regulators of gene expression and key factors in development, disease, and targets for bioengineering. consequently, micrornas have become essential elements of already burgeoning draft plant genome descriptions where their annotation is often particularly poor, contributing unduly to the corruption of public databases. using the citrus sinensis as an example, we highlight and review common failings of mirnaome annotations. understanding and exploiting the role of mirnas ... | 2017 | 28054378 |
| physiologic, anatomic, and gene expression changes in citrus sunki, poncirus trifoliata, and their hybrids after 'candidatus liberibacter asiaticus' infection. | huanglongbing (hlb) is a destructive disease of citrus caused by phloem-limited bacteria, namely 'candidatus liberibacter asiaticus' (las), 'candidatus liberibacter africanus', and 'candidatus liberibacter americanus'. although there are no known hlb-resistant citrus species, studies have reported poncirus trifoliata as being more tolerant. assuming that callose deposition in the phloem of infected plants can inhibit translocation of photosynthetic products and cause starch accumulation, we comp ... | 2017 | 28068188 |
| the first chloroplast genome sequence of boswellia sacra, a resin-producing plant in oman. | boswellia sacra (burseraceae), a keystone endemic species, is famous for the production of fragrant oleo-gum resin. however, the genetic make-up especially the genomic information about chloroplast is still unknown. here, we described for the first time the chloroplast (cp) genome of b. sacra. the complete cp sequence revealed a circular genome of 160,543 bp size with 37.61% gc content. the cp genome is a typical quadripartite chloroplast structure with inverted repeats (irs 26,763 bp) separated ... | 2017 | 28085925 |
| leaf-disc grafting for the transmission of candidatus liberibacter asiaticus in citrus (citrus sinensis; rutaceae) seedlings. | the search for resistance/tolerance to the devastating citrus huanglongbing disease (syn. hlb or citrus greening) is generating an increasing number of new plants of diverse genetic makeup. as the increasing number of new plants require more space, resources, and time, the need for faster and more efficient hlb screening tests becomes crucial. | 2017 | 28090406 |
| effects of foliar application with compost tea and filtrate biogas slurry liquid on yield and fruit quality of washington navel orange (citrus sinenesis osbeck) trees. | sixteen-year-old navel orange trees at a private orchard located in kafer el-sheikh governorate, egypt, were used in this study. compost tea (ct) and filtrate biogas slurry liquid (flb) were applied at two different concentrations (50% and 100%); control trees were sprayed with water. trees treated with ct at 100% were the highest in yield, fruit weight, and vitamin c, whereas the highest percentage of fruit set, fruit number, and soluble solid content (ssc), lowest fruit drop, and highest reduc ... | 2012 | 28102782 |
| tyramine pathways in citrus plant defense: glycoconjugates of tyramine and its n-methylated derivatives. | glucosylated forms of tyramine and some of its n-methylated derivatives are here reported for the first time to occur in citrus genus plants. the compounds tyramine-o-β-d-glucoside, n-methyltyramine-o-β-d-glucoside, and n,n-dimethyltyramine-o-β-d-glucoside were detected in juice and leaves of sweet orange, bitter orange, bergamot, citron, lemon, mandarin, and pomelo. the compounds were identified by mass spectrometric analysis, enzymatic synthesis, and comparison with extracts of stapelia hirsut ... | 2017 | 28117581 |
| ectopic accumulation of linalool confers resistance to xanthomonas citri subsp. citri in transgenic sweet orange plants. | in order to clarify whether high linalool content in citrus leaves alone induces strong field resistance to citrus canker caused by xanthomonas citri subsp. citri (xcc), and to assess whether this trait can be transferred to a citrus type highly sensitive to the bacterium, transgenic 'hamlin' sweet orange (citrus sinensis l. osbeck) plants over-expressing a linalool synthase gene (custs3-1) were generated. transgenic lines (lil) with the highest linalool content showed strong resistance to citru ... | 2017 | 28131994 |
| effect of pasteurization on flavonoids and carotenoids in citrus sinensis (l.) osbeck cv. 'cara cara' and 'bahia' juices. | orange juice is considered an excellent dietary source of several bioactive compounds with beneficial properties for human health. citrus sinensis osbeck cv. 'cara cara' is a bud mutation originated from 'washington' navel orange, also known as 'bahia' navel orange. the ascorbic acid, flavonoid, and carotenoid contents in pasteurized and nonpasteurized bahia and cara cara juices using two lc-ms/ms platforms were investigated. higher ascorbic acid content was observed in bahia compared to cara ca ... | 2017 | 28146357 |
| inducible expression of bs2 r gene from capsicum chacoense in sweet orange (citrus sinensis l. osbeck) confers enhanced resistance to citrus canker disease. | transgenic expression of the pepper bs2 gene confers resistance to xanthomonas campestris pv. vesicatoria (xcv) pathogenic strains which contain the avrbs2 avirulence gene in susceptible pepper and tomato varieties. the avrbs2 gene is highly conserved among members of the xanthomonas genus, and the avrbs2 of xcv shares 96% homology with the avrbs2 of xanthomonas citri subsp. citri (xcc), the causal agent of citrus canker disease. a previous study showed that the transient expression of pepper bs ... | 2017 | 28155188 |
| depletion of abscisic acid levels in roots of flooded carrizo citrange (poncirus trifoliata l. raf. × citrus sinensis l. osb.) plants is a stress-specific response associated to the differential expression of pyr/pyl/rcar receptors. | soil flooding reduces root abscisic acid (aba) levels in citrus, conversely to what happens under drought. despite this reduction, microarray analyses suggested the existence of a residual aba signaling in roots of flooded carrizo citrange seedlings. the comparison of aba metabolism and signaling in roots of flooded and water stressed plants of carrizo citrange revealed that the hormone depletion was linked to the upregulation of csaog, involved in aba glycosyl ester (abage) synthesis, and to a ... | 2017 | 28160166 |
| encapsulation of polymethoxyflavones in citrus oil emulsion-based delivery systems. | the purpose of this work was to elucidate the effects of citrus oil type on polymethoxyflavone (pmf) solubility and on the physicochemical properties of pmf-loaded emulsion-based delivery systems. citrus oils were extracted from mandarin, orange, sweet orange, and bergamot. the major constituents were determined by gc/ms: sweet orange oil (97.4% d-limonene); mandarin oil (72.4% d-limonene); orange oil (67.2% d-limonene); and bergamot oil (34.6% linalyl acetate and 25.3% d-limonene). pmf-loaded e ... | 2017 | 28182421 |
| expression of bacillus thuringiensis cytolytic toxin (cyt2ca1) in citrus roots to control diaprepes abbreviatus larvae. | diaprepes abbreviatus (l.) is an important pest of citrus in the usa. currently, no effective management strategies of d. abbreviatus exist in citriculture, and new methods of control are desperately sought. to protect citrus against d. abbreviatus a transgenic citrus rootstock expressing bacillus thuringiensis cyt2ca1, an insect toxin protein, was developed using agrobacterium-mediated transformation of 'carrizo' citrange [citrus sinensis (l) osbeck poncirus trifoliate (l) raf]. the transgenic ... | 2017 | 28187824 |
| production and characterization of films based on blends of chitosan from blue crab (callinectes sapidus) waste and pectin from orange (citrus sinensis osbeck) peel. | the objective of this study was to develop and characterize films based on blends of chitosan and pectin, produced in laboratory scale, from industrial wastes. the chitosan was obtained by termoalcaline deacetylation of chitin, extracted from blue crab (callinectes sapidus) waste and characterized according to degree of deacetylation (dd) and viscosimetric molecular weight (mw); and pectin was extracted by conventional heating, from orange (citrus sinensis osbeck) peel and characterized accordin ... | 2017 | 28189792 |
| influence of orange cultivar and mandarin postharvest storage on polyphenols, ascorbic acid and antioxidant activity during gastrointestinal digestion. | polyphenols, ascorbic acid content and antioxidant activity of two sweet oranges (navel-n and cara cara-cc) and mandarin (clementine-m) as well as their bioaccessibilities were evaluated in pulps and compared to those in fresh juice. thus, pulps of oranges and mandarins displayed higher hesperidin (hes), narirutin (nar), total flavonoids (tf), total phenols (tp) and antioxidant activity (aac) than their corresponding juices. also, cc products presented higher bioactive compounds content than n o ... | 0 | 28193404 |
| changes in anthocyanin production during domestication of citrus. | mandarin (citrus reticulata), citron (citrus medica), and pummelo (citrus maxima) are important species of the genus citrus and parents of the interspecific hybrids that constitute the most familiar commercial varieties of citrus: sweet orange, sour orange, clementine, lemon, lime, and grapefruit. citron produces anthocyanins in its young leaves and flowers, as do species in genera closely related to citrus, but mandarins do not, and pummelo varieties that produce anthocyanins have not been repo ... | 2017 | 28196843 |
| natural compounds from mexican medicinal plants as potential drug leads for anti-tuberculosis drugs. | in mexican traditional medicine 187 plant species are used in the treatment of respiratory conditions that may be associated with tuberculosis. in this contribution, we review the ethnobotany, chemistry and pharmacology of 63 species whose extracts have been assayed for antimycobacterial activity in vitro. among these, the most potent is aristolochia brevipes (mic= 12.5 µg/ml), followed by aristolochia taliscana, citrus sinensis, chrysactinia mexicana, persea americana, and olea europaea (mic<64 ... | 2017 | 28198919 |
| two previously unknown phytophthora species associated with brown rot of pomelo (citrus grandis) fruits in vietnam. | two distinct phytophthora taxa were found to be associated with brown rot of pomelo (citrus grandis), a new disease of this ancestral citrus species, in the vinh long province, mekong river delta area, southern vietnam. on the basis of morphological characters and using the its1-5.8s-its2 region of the rdna and the cytochrome oxidase subunit 1 (coi) as barcode genes, one of the two taxa was provisionally named as phytophthora sp. prodigiosa, being closely related to but distinct from p. insolita ... | 2017 | 28208159 |
| experimental evidence and in silico identification of tryptophan decarboxylase in citrus genus. | plant tryptophan decarboxylase (tdc) converts tryptophan into tryptamine, precursor of indolealkylamine alkaloids. the recent finding of tryptamine metabolites in citrus plants leads to hypothesize the existence of tdc activity in this genus. here, we report for the first time that, in citrus x limon seedlings, deuterium labeled tryptophan is decarboxylated into tryptamine, from which successively deuterated n,n,n-trimethyltryptamine is formed. these results give an evidence of the occurrence of ... | 2017 | 28208655 |
| changes in volatile and non-volatile flavor chemicals of "valencia" orange juice over the harvest seasons. | florida "valencia" oranges have a wide harvest window, covering four months after first reaching the commercial maturity. however, the influence of harvest time on juice flavor chemicals is not well documented, with the exception of sugars and acids. therefore, we investigated the major flavor chemicals, volatile (aroma), non-volatile (taste) and mouth feel attributes, in the two harvest seasons (march to june in 2007 and february to may in 2012). bitter limonoid compounds, limonin and nomilin, ... | 2016 | 28231099 |
| accurate measurement of transgene copy number in crop plants using droplet digital pcr. | genetic transformation is a powerful means for the improvement of crop plants, but requires labor- and resource-intensive methods. an efficient method for identifying single-copy transgene insertion events from a population of independent transgenic lines is desirable. currently, transgene copy number is estimated by either southern blot hybridization analyses or quantitative polymerase chain reaction (qpcr) experiments. southern hybridization is a convincing and reliable method, but it also is ... | 2017 | 28231382 |
| fast separation and sensitive quantitation of polymethoxylated flavonoids in the peels of citrus using uplc-q-tof-ms. | a rapid, sensitive, and efficient ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (uplc-q-tof-ms) method has been developed to analyze polymethoxylated flavonoids (pmfs) in 14 citrus peels, including 7 citrus reticulata (c. reticulata) and 7 citrus sinensis (c. sinensis). in this study, fast separation can be achieved within 12 min and 42 pmfs have been identified including 33 flavones and 9 flavanones. most c. reticulata were shown to contain more ... | 2017 | 28260382 |
| effects of high toxic boron concentration on protein profiles in roots of two citrus species differing in boron-tolerance revealed by a 2-de based ms approach. | citrus are sensitive to boron (b)-toxicity. in china, b-toxicity occurs in some citrus orchards. so far, limited data are available on b-toxicity-responsive proteins in higher plants. thirteen-week-old seedlings of "sour pummelo" (citrus grandis) and "xuegan" (citrus sinensis) was fertilized every other day until dripping with nutrient solution containing 10 μm (control) or 400 μm (b-toxicity) h3bo3 for 15 weeks. the typical b-toxic symptom only occurred in 400 μm b-treated c. grandis leaves, an ... | 2017 | 28261239 |
| physicochemical and sensory quality of yogurt incorporated with pectin from peel of citrus sinensis. | industrial by-product like orange peel plays an important role in pectin manufacture. the objective of this article was to extract pectin from peel of citrus sinensis and to study the effect of its incorporation on the quality of yogurt during the period of fermentation and postacidification. physicochemical, organoleptic, and rheological properties of yogurt prepared with pectin were studied in order to determine the best preparation depending on the rate of pectin. the extraction pectin yield ... | 2017 | 28265371 |
| real-time genetic manipulations of the cytokinin pathway: a tool for laboratory and field studies. | although many established tools for cytokinin (ck) pathway manipulations are well suitable for the analysis of molecular interactions, their use on a whole plant scale is often limited by the induction of severe developmental defects. to circumvent this problem, different methods were developed that allow for a more precise manipulation of the ck pathway. here we present one of these systems, the pop6/lhgr system for chemically inducible gene expression. this system allows regulation on a spatia ... | 2017 | 28265993 |
| differentiation using microwave plasma torch desorption mass spectrometry of navel oranges cultivated in neighboring habitats. | the molecular fingerprinting of intact fruit samples combined with statistical data analysis can allow the assessment of fruit quality and location of origin. herein, microwave plasma torch desorption ionization mass spectrometry (mpt-ms) was applied to produce molecular fingerprints for the juice sac and exocarp of navel oranges cultivated in three closely located habitats, and the mass spectrometric fingerprints were differentiated by principal component analysis (pca). because of the relative ... | 0 | 28269986 |
| effects of low ph on photosynthesis, related physiological parameters, and nutrient profiles of citrus. | seedlings of "xuegan" (citrus sinensis) and "sour pummelo" (citrus grandis) were irrigated daily with a nutrient solution at a ph of 2.5, 3, 4, 5, or 6 for 9 months. thereafter, the following responses were investigated: seedling growth; root, stem, and leaf concentrations of nutrient elements; leaf gas exchange, pigment concentration, ribulose-1,5-bisphosphate carboxylase/oxygenase activity and chlorophyll a fluorescence; relative water content, total soluble protein level, h2o2 production and ... | 2017 | 28270819 |
| functional and structural characterization of a (+)-limonene synthase from citrus sinensis. | terpenes make up the largest and most diverse class of natural compounds and have important commercial and medical applications. limonene is a cyclic monoterpene (c10) present in nature as two enantiomers, (+) and (-), which are produced by different enzymes. the mechanism of production of the (-)-enantiomer has been studied in great detail, but to understand how enantiomeric selectivity is achieved in this class of enzymes, it is important to develop a thorough biochemical description of enzyme ... | 2017 | 28272875 |
| structural characterization of early michaelis complexes in the reaction catalyzed by (+)-limonene synthase from citrus sinensis using fluorinated substrate analogues. | the stereochemical course of monoterpene synthase reactions is thought to be determined early in the reaction sequence by selective binding of distinct conformations of the geranyl diphosphate (gpp) substrate. we explore here formation of early michaelis complexes of the (+)-limonene synthase [(+)-ls] from citrus sinensis using monofluorinated substrate analogues 2-fluoro-gpp (fgpp) and 2-fluoroneryl diphosphate (fnpp). both are competitive inhibitors for (+)-ls with ki values of 2.4 ± 0.5 and 3 ... | 2017 | 28272876 |
| sclerenchymatous ring as a barrier to phloem feeding by asian citrus psyllid: evidence from electrical penetration graph and visualization of stylet pathways. | asian citrus psyllid (diaphorina citri) feeding behaviors play a significant role in the transmission of the phloem-limited candidatus liberibacter asiaticus (clas) bacterium that causes the economically devastating citrus greening disease. sustained phloem ingestion by d. citri on clas infected plants is required for pathogen acquisition and transmission. recent studies have shown a fibrous ring of thick-walled sclerenchyma around the phloem in mature, fully expanded citrus leaves that is more ... | 2017 | 28278248 |
| investigation on key molecules of huanglongbing (hlb)-induced orange juice off-flavor. | orange fruits from huanglongbing (hlb)-infected trees do not fully mature and show a severe off-flavor described as bitter-harsh, metallic, and less juicy and fruity. the investigation of juice from hlb-infected (hlboj) and healthy control oranges (coj) by gas chromatography-mass spectrometry showed higher concentrations of fruity esters, such as ethyl butyrate and ethyl 2-methylbutyrate, and soapy-waxy alkanals, such as octanal and decanal, in the coj, whereas the hlboj showed higher concentrat ... | 2017 | 28285516 |
| effect of physiological and experiential state ofbactrocera tryoni flies on intra-tree foraging behavior for food (bacteria) and host fruit. | using caged host trees on which we manipulated food and oviposition sites, we investigated the foraging behavior of individually-releasedbactrocera tryoni (diptera: tephritidae) females in relation to state of fly hunger for protein, presence or absence of bacteria as a source of protein, degree of prior experience with host fruit, and quality of host fruit for oviposition. one aim was to evaluate whether it is immature or matureb. tryoni females that are responsible for initially inoculating ho ... | 1991 | 28313268 |
| root adaptive responses to aluminum-treatment revealed by rna-seq in two citrus species with different aluminum-tolerance. | seedlings of aluminum (al)-tolerant citrus sinensis and al-intolerant citrus grandis were fertigated daily with nutrient solution containing 0 and 1.0 mm alcl3●6h2o for 18 weeks. the al-induced decreases of biomass and root total soluble proteins only occurred in c. grandis, demonstrating that c. sinensis had higher al-tolerance than c. grandis. under al-treatment, c. sinensis roots secreted more citrate and malate than c. grandis ones; less al was accumulated in c. sinenis than in c. grandis le ... | 2017 | 28337215 |
| transcriptome changes associated with boron deficiency in leaves of two citrus scion-rootstock combinations. | boron (b) deficiency stress is frequently observed in citrus orchards and causes considerable loss of productivity and fruit quality. carrizo citrange (cc) has been reported as a rootstock more tolerant to b deficiency than trifoliate orange (to). the 'newhall' navel orange (ns) performed better when grafted onto cc (ns/cc) than when grafted onto to (ns/to) under long-term b deficiency. the present study confirmed that ns/cc had higher boron content, leaf fresh weight, lower leaf chlorosis and s ... | 2017 | 28352276 |
| spasmolytic and antibacterial activity of two citrus sinensis osbeck varieties cultivated in mexico. | antibacterial activity on atcc strains of escherichia coli, salmonella enterica, salmonella enteritidis, and salmonella choleraesuis and spasmolytic effect on contraction on rat ileum trips were determinate. eight organic extracts (hexanic and methanolic) of albedo (mesocarp) and flavedo (pericarp) of two varieties (valencian and national) of citrus sinensis (l.) osbeck of yucatán, méxico, were studied. additionally, chromatographic fingerprints were obtained and correlated with their pharmacolo ... | 2017 | 28356916 |
| one target, two mechanisms: the impact of candidatus liberibacter asiaticus and its vector, diaphorina citri on citrus leaf pigments. | huanglongbing is currently the largest threat to global citrus production. we examined the effect of hlb pathogen, candidatus liberibacter asiaticus-infection and/or its vector, diaphorina citri-infestation on valencia sweet orange leaf pigments using hplc, followed by gene expression analysis for 46 involved genes in carotenoids- and chlorophylls-biosynthesis pathways. both clas and d. citri alter the total citrus leaf pigments balance with a greater impact by clas. while zeaxanthin was accumul ... | 2017 | 28358623 |
| comparison of two dna extraction protocols from leave samples of cotinus coggygria, citrus sinensis and genus juglans. | high quality dna is essential for molecular research. secondary metabolites can affect the quantity and quality dna. in current research two dna isolation methods including ctab and delaporta (protocols 1 & 2 respectively) were applied in three leave samples from cotinus coggygria, citrus sinensis and genus juglans that their leaves are rich of secondary metabolites. we successfully isolated dna from c. coggygria, c. sinensis and genus juglans using the two protocols described above. good qu ... | 2017 | 28364796 |
| engineering canker-resistant plants through crispr/cas9-targeted editing of the susceptibility gene cslob1 promoter in citrus. | citrus canker, caused by xanthomonas citri subsp. citri (xcc), is severely damaging to the global citrus industry. targeted editing of host disease-susceptibility genes represents an interesting and potentially durable alternative in plant breeding for resistance. here, we report improvement of citrus canker resistance through crispr/cas9-targeted modification of the susceptibility gene cslob1 promoter in citrus. wanjincheng orange (citrus sinensis osbeck) harbors at least three copies of the cs ... | 2017 | 28371200 |
| insecticidal and repellent activity of several plant-derived essential oils against aedes aegypti. | we examined the pupicidal, adulticidal, repellent, and oviposition-deterrent activities of essential oils (eos) from lippia alba, l. origanoides, eucalyptus citriodora, cymbopogon citratus, cymbopogon flexuosus, citrus sinensis , cananga odorata , swinglea glutinosa, and tagetes lucida plants against aedes aegypti under laboratory conditions. pupicidal and adulticidal activities were assessed at exploratory concentrations of 250, 310, and 390 parts per million (ppm); and 30, 300, and 1,000 ppm, ... | 2017 | 28388322 |
| kinetic and isotherm studies of bisphenol a adsorption onto orange albedo(citrus sinensis): sorption mechanisms based on the main albedo components vitamin c, flavones glycosides and carotenoids. | orange albedo and its adsorption capacity towards bisphenol a (bpa) were studied. adsorption experiments were conducted in batch mode at 25-55°c. scanning electron microscopy (sem), energy dispersive x-ray spectroscopy (edx), x-ray diffraction (xrd), brunauer-emmett-teller (bet) and fourier transform infrared (ftir) spectroscopy were used to characterise the biosorbent. the effects of various parameters including adsorption time, equilibrium ph, adsorbent dosage and initial adsorbate concentrati ... | 2017 | 28394738 |
| cytological and molecular characterization of carotenoid accumulation in normal and high-lycopene mutant oranges. | ripe cara cara sweet orange contains 25 times as much carotenoids in flesh as newhall sweet orange, due to high accumulation of carotenes, mainly phytoene, lycopene and phytofluene. only yellow globular chromoplasts were observed in newhall flesh. distinct yellow globular and red elongated crystalline chromoplasts were found in cara cara but only one type of chromoplast was present in each cell. the red crystalline chromoplasts contained lycopene as a dominant carotenoid and were associated with ... | 2017 | 28396598 |
| citrus leprosis virus n: a new dichorhavirus causing citrus leprosis disease. | citrus leprosis (cl) is a viral disease endemic to the western hemisphere that produces local-necrotic and -chlorotic lesions on leaves, branches and fruits and causes serious yield reduction in citrus orchards. samples of sweet orange (citrus × sinensis) trees showing cl symptoms were collected during a survey in non-commercial citrus areas in the southeast region of brazil in 2013-2016. transmission electron microscopy analyses of foliar lesions confirmed the presence of rod-like viral particl ... | 2017 | 28398876 |
| qtl mapping for fruit quality in citrus using dartseq markers. | citrus breeding programs have many limitations associated with the species biology and physiology, requiring the incorporation of new biotechnological tools to provide new breeding possibilities. diversity arrays technology (dart) markers, combined with next-generation sequencing, have wide applicability in the construction of high-resolution genetic maps and in quantitative trait locus (qtl) mapping. this study aimed to construct an integrated genetic map using full-sib progeny derived from mur ... | 0 | 28403819 |
| influence of postharvest treatments on qualitative and chemical parameters of tarocco blood orange fruits to be used for fresh chilled juice. | tarocco "sant'alfio" is a late ripening blood orange cultivar. blood oranges are more and more appreciated from consumers for their high nutraceutical value due to the presence of bioactive compounds including vitamin c, polyphenols, flavonoids and hydroxycinnamic acids. the aim of this work is to set up a reliable protocol for postharvest storage of the very-late tarocco "sant'alfio" orange to prolong the availability of this product in the market to be used for fresh chilled orange juice produ ... | 0 | 28407933 |
| bioavailable citrus sinensis extract: polyphenolic composition and biological activity. | citrus plants contain large amounts of flavonoids with beneficial effects on human health. in the present study, the antioxidant and anti-inflammatory potential of bioavailable polyphenols from citrus sinensis was evaluated in vitro and ex vivo, using the murine macrophages cell line j774a.1 and primary peritoneal macrophages. following simulated gastro-intestinal digestion, the in vitro bioavailability of citrus sinensis polyphenolic extract was assessed using the human cell line caco-2 grown a ... | 2017 | 28420125 |
| fumigant activity of sweet orange essential oil fractions against red imported fire ants (hymenoptera: formicidae). | sweet orange oil fractions were prepared by molecular distillation of cold-pressed orange oil from sample a (citrus sinensis (l.) 'hamlin' from america) and sample b (citrus sinensis osbeck 'newhall' from china) respectively, and their fumigant activities against medium workers of red imported fire ants (solenopsis invicta buren) were investigated. the volatile composition of the orange oil fractions was identified and quantified using gc-ms. fractions from sample a (a1, a2, and a3) contained 23 ... | 2017 | 28444322 |
| identification and characterization of citrus tristeza virus isolates breaking resistance in trifoliate orange in california. | most citrus tristeza virus (ctv) isolates in california are biologically mild and symptomless in commercial cultivars on ctv tolerant rootstocks. however, to better define california ctv isolates showing divergent serological and genetic profiles, selected isolates were subjected to deep sequencing of small rnas. full-length sequences were assembled, annotated and trifoliate orange resistance-breaking (rb) isolates of ctv were identified. phylogenetic relationships based on their full genomes pl ... | 2017 | 28453412 |
| alternative oxidase (aox) constitutes a small family of proteins in citrus clementina and citrus sinensis l. osb. | the alternative oxidase (aox) protein is present in plants, fungi, protozoa and some invertebrates. it is involved in the mitochondrial respiratory chain, providing an alternative route for the transport of electrons, leading to the reduction of oxygen to form water. the present study aimed to characterize the family of aox genes in mandarin (citrus clementina) and sweet orange (citrus sinensis) at nucleotide and protein levels, including promoter analysis, phylogenetic analysis and c. sinensis ... | 2017 | 28459876 |
| systems biology study of transcriptional and post-transcriptional co-regulatory network sheds light on key regulators involved in important biological processes in citrus sinensis. | transcriptional and post-transcriptional regulators including transcription regulator, transcription factor and mirna are the main endogenous molecular elements which control complex cellular mechanisms such as development, growth and response to biotic and abiotic stresses in a coordinated manner in plants. utilizing the most recent information on such relationships in a plant species, obtained from high-throughput experimental technologies and advanced computational tools, we can reconstruct i ... | 2017 | 28461722 |
| quantification of transfer of salmonella from citrus fruits to peel, edible portion, and gloved hands during hand peeling. | although studies have quantified bacterial transfer between hands and various materials, cross-contamination between the surface of fresh citrus fruit and the edible portions during hand peeling has not been reported. this study quantifies transfer of salmonella to the edible portion of citrus fruit from a contaminated peel during hand peeling. citrus fruits used for this study were citrus sinensis (sweet orange) cultivars 'valencia' and 'navel', citrus unshiu (satsuma mandarins), citrus reticul ... | 2017 | 28463082 |
| compositional shift in fatty acid profiles of lipids obtained from oleaginous yeasts upon the addition of essential oil from citrus sinensis l. | tailoring lipids from oleaginous yeasts to contain specific types of fatty acid is of considerable interest to food, fuel, and pharmaceutical industries. in this study, the essential oil obtained from citrus sinesus l. has been used to alter the fatty acid composition of two common oleaginous yeasts, rhodosporidium toruloides and cryptococcus curvatus. with increasing levels of essential oil in the medium, the metabolic flux of the fatty acid biosynthesis pathway shifted towards saturated fatty ... | 2017 | 28474217 |
| tetraploid carrizo citrange rootstock (citrus sinensis osb.×poncirus trifoliata l. raf.) enhances natural chilling stress tolerance of common clementine (citrus clementina hort. ex tan). | low temperatures can disturb the development, growth and geographic distribution of plants, particularly cold-sensitive plants in the mediterranean area, where temperatures can reach seasonally low levels. in citrus crops, scion/rootstock combinations are used to improve fruit production and quality, and increase tolerance to biotic and abiotic stresses. in the last decade, several studies have shown that tetraploid citrus seedlings or rootstocks are more tolerant to abiotic stress than their re ... | 2017 | 28478318 |
| anti-quorum sensing activity of some medicinal plants. | quorum sensing is the key regulator of virulence factors of pseudomonas aeruginosa such as biofilm formation, motility, productions of proteases, hemolysin, pyocyanin, and toxins. the aim of this study was to explore the effect of the extracts from some medicinal plants on quorum sensing and related virulence factors of p. aeruginosa. | 2016 | 28487896 |
| development of monoclonal antibody-based elisa for the quantification of orange allergen cit s 2 in fresh and processed oranges. | the aim of this study was to develop a monoclonal antibody (mab)-based enzyme-linked immunosorbent assay (elisa) for the quantification of a major allergen (cit s 2) in fresh and processed oranges. purified recombinant cit s 2 (rcit s 2)-small ubiquitin-like modifier (sumo) was used for the production of mabs. in the optimized elisa, the recovery of rcit s 2 from navel oranges or orange juice was 107-132%, and the intra- and inter-assay coefficients of variation were 3.1-8.8% and 4.4-11%, respec ... | 2017 | 28490094 |
| carotenoid profiling of red navel orange "cara cara" harvested from five regions in china. | understanding the composition and content of carotenoids in red-flesh navel orange "cara cara" cultivated in different regions is valuable for its nutrition assessment and fruit processing control. herein, the carotenoids of "cara cara" fruits, harvested from five typical citrus growing regions of china, were identified and quantified by hplc-dad coupled with hplc-dad-apci-ms/ms. in saponified extracts, 19 free carotenoids including 8 carotenes and 11 xanthophylls were identified. in unsaponifie ... | 0 | 28490141 |
| metabolic profile and underlying improved bio-activity of fructus aurantii immaturus by human intestinal bacteria. | fructus aurantii immaturus (fai) is the dried young fruit of citrus aurantium l. or citrus sinensis l. osbeck. the purpose of this paper was to investigate the metabolic fate of fai upon incubation with human intestinal bacteria, meanwhile to evaluate the antioxidant and anti-inflammatory activities of fai and the transformed fructus aurantii immaturus (tfai). the water extract of fai was anaerobically incubated with human intestinal bacterial suspensions for 48 h at 37 °c. liquid chromatography ... | 2017 | 28504280 |
| isolation and identification of fungi associated with spoilt fruits vended in gwagwalada market, abuja, nigeria. | annual reports have shown that 20% of fruits and vegetables produced are lost to spoilage. this study was undertaken to isolate and identify fungi that are associated with spoilt fruits commonly sold in gwagwalada market, abuja, and recommend appropriate control measure. | 2017 | 28507410 |
| metabolomic response to huanglongbing: role of carboxylic compounds in citrus sinensis response to 'candidatus liberibacter asiaticus' and its vector, diaphorina citri. | huanglongbing, a destructive disease of citrus, is caused by the fastidious bacterium 'candidatus liberibacter asiaticus' and transmitted by asian citrus psyllid, diaphorina citri. the impact of 'ca. l. asiaticus' infection or d. citri infestation on valencia sweet orange (citrus sinensis) leaf metabolites was investigated using gas chromatography mass spectrometry, followed by gene expression analysis for 37 genes involved in jasmonic acid (ja), salicylic acid (sa), and proline-glutamine pathwa ... | 2017 | 28510485 |
| cytotoxicity and anti-leishmania amazonensis activity of citrus sinensis leaf extracts. | leishmania amazonensis is the main agent of diffuse cutaneous leishmaniasis, a disease characterized by lesional polymorphism and the commitment of skin surface. previous reports demonstrated that the citrus genus possess antimicrobial activity. | 2017 | 28524774 |
| spray pesticide applications in mediterranean citrus orchards: canopy deposition and off-target losses. | only a portion of the water volume sprayed is deposited on the target when applying plant protection products with air-assisted axial-fan airblast sprayers in high growing crops. a fraction of the off-target losses deposits on the ground, but droplets also drift away from the site. this work aimed at assessing the spray distribution to different compartments (tree canopy, ground and air) during pesticide applications in a mediterranean citrus orchard. standard cone nozzles (teejet d3 dc35) and v ... | 2017 | 28525940 |
| in silico identification and validation of potential micrornas in kinnow mandarin (citrus reticulata blanco). | micrornas (mirnas) are a large family of 19-25 nucleotides, regulatory, non-coding rna molecules that control gene expression by cleaving or inhibiting the translation of target gene transcripts in animals and plants. despite the important functions of mirnas related to regulation of plant growth and development processes, metabolism, and abiotic and biotic stresses, little is known about the disease-related mirna. here, we present a new pipeline for mirna analysis using expressed sequence tags ... | 2017 | 28534166 |
| different adaptation strategies of two citrus scion/rootstock combinations in response to drought stress. | scion/rootstock interaction is important for plant development and for breeding programs. in this context, polyploid rootstocks presented several advantages, mainly in relation to biotic and abiotic stresses. here we analyzed the response to drought of two different scion/rootstock combinations presenting different polyploidy: the diploid (2x) and autotetraploid (4x) rangpur lime (citrus limonia, osbeck) rootstocks grafted with 2x valencia delta sweet orange (citrus sinensis) scions, named v/2xr ... | 2017 | 28545114 |
| sulfate-binding protein (sbp) from xanthomonas citri: structure and functional insights. | the uptake and transport of sulfate in bacteria is mediated by an atp-binding cassette transporter (abc transporter) encoded by sbpcysuwa genes, whose importance has been widely demonstrated due to their relevance in cysteine synthesis and bacterial growth. in xanthomonas citri, the causative agent of canker disease, the expression of components from this abc transporter and others related to uptake of organic sulfur sources has been shown during in vitro growth cultures. in this work, based on ... | 2017 | 28562158 |
| comparative study on ambient ionization methods for direct analysis of navel orange tissues by mass spectrometry. | it is of sustainable interest to improve the sensitivity and selectivity of the ionization process, especially for direct analysis of complex samples without matrix separation. herein, four ambient ionization methods including desorption atmospheric pressure chemical ionization (dapci), heat-assisted desorption atmospheric pressure chemical ionization (heat-assisted dapci), microwave plasma torch (mpt), and internal extractive electrospray ionization (ieesi) were employed for comparative analysi ... | 0 | 28628730 |
| magnesium-deficiency-induced alterations of gas exchange, major metabolites and key enzymes differ among roots, and lower and upper leaves of citrus sinensis seedlings. | magnesium (mg)-deficiency is a widespread problem adversely affecting the quality and yield of crops, including citrus. 'xuegan' [citrus sinensis (l.) osbeck] seedlings were irrigated every other day with nutrient solution at an mg concentration of 0 mm (mg-deficiency) or 1 mm (mg-sufficiency) for 16 weeks. thereafter, biomass, leaf mass per area, ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco), pigments in the upper and lower leaves, mg, gas exchange, organic acids, nonstructural carb ... | 2017 | 28633366 |
| effects of heat treatment on the antioxidative and anti-inflammatory properties of orange by-products. | this study investigated the changes in the functional components, antioxidative activities, antibacterial activities, anti-inflammatory activities of orange (citrus sinensis (l.) osbeck) by-products (obp) on heat treatment at 50 and 100 °c (hereafter denoted 50d and 100d extracts, respectively). optimal heating conditions were also investigated. the total phenolic content, flavonoid content and antioxidative activities of obp extracts significantly increased on heat treatment. the lag time of cu ... | 2017 | 28653733 |
| enzymatic process enhances the flavour profile and increases the proportion of esters in citrus essential oils. | citrus essential oils (ceos) are important flavors in the food and confectionary industries. a lipase process was proposed for enhancing the flavor profiles and increasing the proportions of esters in ceos. the effects of the enzymatic process were explored by detecting the constituents of the ceos of american sweet orange oil (aso) and brazil mandarin oil (bmo) through gc/ms and sensory evaluation by a trained panel, and positive effects were confirmed by both methods. a further eleven kinds of ... | 2017 | 28672071 |
| flavonoid composition of tarocco (citrus sinensis l. osbeck) clone "lempso" and fast antioxidant activity screening by dpph-uhplc-pda-it-tof. | clonal selection and hybridisation are valid strategies to obtain fruits with enhanced sensorial and nutraceutical properties. within citrus sinensis varieties, tarocco clone "lempso" is a typical product of the calabria region (italy) characterised by its red pulp. this is the first report concerning its accurate profiling. | 2017 | 28686312 |
| proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of xanthomonas citri subsp. citri during citrus sinensis infection. | xanthomonas citri subsp. citri (xac) is the causal agent of citrus canker. a proteomic analysis under in planta infectious and non-infectious conditions was conducted in order to increase our knowledge about the adaptive process of xac during infection. | 2017 | 28693412 |
| phylogenetic studies and comparative chloroplast genome analyses elucidate the basal position of halophyte nitraria sibirica (nitrariaceae) in the sapindales. | nitraria sibirica is a halophyte and belongs to the family nitrariaceae. the chloroplast genome of nitraria sibirica (159,466 bp) has a quadripartite structure, which consists of a large single-copy (87,914 bp) region, a small single-copy (18,316 bp) region, and a pair of inverted repeats (26,618 bp). sequencing analyses indicate that the chloroplast genome contains 113 distinct genes, including 79 peptide-coding genes, 30 transfer rna genes, and 4 ribosomal rna genes. we also identified 105 per ... | 2017 | 28712318 |
| anticancer potential of citrus juices and their extracts: a systematic review of both preclinical and clinical studies. | background: during the last decades, a huge body of evidence has been accumulated suggesting that citrus fruits and their juices might have a role in preventing many diseases including cancer. objective: to summarize the numerous evidences on the potential of citrus juices and their extracts as anticancer agents. data sources: a systematic review of articles written in english using medline (1946-present), embase (1974-present) and web of sciences (1970-present) was performed independently by tw ... | 2017 | 28713272 |
| first report of phyllosticta citricarpa and description of two new species, p. paracapitalensis and p. paracitricarpa, from citrus in europe. | the genus phyllosticta occurs worldwide, and contains numerous plant pathogenic, endophytic and saprobic species. phyllosticta citricarpa is the causal agent of citrus black spot disease (cbs), affecting fruits and leaves of several citrus hosts (rutaceae), and can also be isolated from asymptomatic citrus tissues. citrus black spot occurs in citrus-growing regions with warm summer rainfall climates, but is absent in countries of the european union (eu). phyllosticta capitalensis is morphologica ... | 2017 | 28720979 |
| comparative analysis of juice volatiles in selected mandarins, mandarin relatives and other citrus genotypes. | citrus fruit flavor is an important attribute prioritized in variety improvement. the present study compared juice volatiles compositions from 13 selected citrus genotypes, including six mandarins (citrus reticulata), three sour oranges (citrus aurantium), one blood orange (citrus sinensis), one lime (citrus limonia), one clementine (citrus clementina) and one satsuma (citrus unshiu). | 2018 | 28731231 |
| mir3954 is a trigger of phasirnas that affects flowering time in citrus. | in plant, a few 22-nt mirnas direct cleavages of their targets and trigger the biogenesis of phased small interfering rnas (phasirnas) in plant. in this study, we characterized a mirna triggering phasirnas generation, mir3954, and explored its downstream target genes and potential function. our results demonstrated that mir3954 showed specific expression in the flowers of citrus species, and it targeted a nac transcription factor (cs7 g22460) and two non-coding rna transcripts (lncrnas, cs1 g096 ... | 2017 | 28749585 |
| physicochemical and sensory properties of cold pressed oils from florida hamlin and valencia oranges affected by huanglongbing. | cold pressed oils from huanglongbing (hlb) symptomatic (sy) and asymptomatic (as) hamlin and valencia oranges were assessed for 2 y (2014 to 2015 and 2015 to 2016 seasons) with 2 harvest dates for each orange variety per year. physicochemical properties (optical rotation, aldehyde content, ultraviolet [uv] absorbance, refractive index, and specific gravity) mandated by the united states pharmacopeia (usp) for orange oil quality were assessed. hamlin and valencia oils showed minor differences in ... | 2017 | 28759106 |
| navel orange peel hydroethanolic extract, naringin and naringenin have anti-diabetic potentials in type 2 diabetic rats. | the therapy of type 2 diabetes mellitus (t2dm) stays a challenging issue. during the last decade, there has been an interest in the expansion of anti-diabetic drugs especially those of natural sources. thus, the aim of this study was to assess the anti-hyperglycemic and the anti-hyperlipidemic effects as well as the anti-oxidant activities of navel orange hydroethanolic extract and its constituting flavonoids naringin and naringenin on nicotineamide (na)/streptozotocin (stz)-induced type 2 diabe ... | 2017 | 28759757 |
| genome-wide identification of sweet orange (citrus sinensis) metal tolerance proteins and analysis of their expression patterns under zinc, manganese, copper, and cadmium toxicity. | plant metal tolerance proteins (mtps) play important roles in heavy metal homeostasis; however, related information in citrus plants is limited. citrus genome sequencing and assembly have enabled us to perform a systematic analysis of the mtp gene family. we identified 12 mtp genes in sweet orange, which we have named as citmtp1 and citmtp3 to citmtp12 based on their sequence similarity to arabidopsis thaliana mtps. the citmtps were predicted to encode proteins of 864 to 2556 amino acids in leng ... | 2017 | 28760553 |
| anthocyanin biosynthesis and accumulation in blood oranges during postharvest storage at different low temperatures. | blood oranges require low temperature for anthocyanin production. we have investigated the activation of anthocyanin biosynthesis and accumulation in the pulp of moro blood and pera blond oranges (citrus sinensis l. osbeck) stored at either 4 or 9°c after harvesting. both temperatures stimulated anthocyanin accumulation in blood but not in blond oranges. nonetheless, blood orange fruits stored at 9°c reached a darker purple coloration, higher anthocyanin contents and enhanced upregulation of gen ... | 2017 | 28764055 |
| ectopic expression of xylella fastidiosa rpff conferring production of diffusible signal factor in transgenic tobacco and citrus alters pathogen behavior and reduces disease severity. | the pathogenicity of xylella fastidiosa is associated with its ability to colonize the xylem of host plants. expression of genes contributing to xylem colonization are suppressed, while those necessary for insect vector acquisition are increased, with increasing concentrations of diffusible signal factor (dsf) whose production is dependent on rpff. we previously demonstrated that transgenic citrus plants ectopically expressing rpff from a citrus strain of x. fastidiosa subsp. pauca exhibited les ... | 2017 | 28777044 |
| crystal structure and regulation of the citrus pol iii repressor maf1 by auxin and phosphorylation. | maf1 is the main rna polymerase (pol) iii repressor that controls cell growth in eukaryotes. the citrus ortholog, csmaf1, was shown to restrict cell growth in citrus canker disease but its role in plant development and disease is still unclear. we solved the crystal structure of the globular core of csmaf1, which reveals additional structural elements compared with the previously available structure of hmaf1, and explored the dynamics of its flexible regions not present in the structure. csmaf1 ... | 2017 | 28781084 |