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a trojan-horse peptide-carboxymethyl-cytidine antibiotic from bacillus amyloliquefaciens.microcin c and related antibiotics are trojan-horse peptide-adenylates. the peptide part is responsible for facilitated transport inside the sensitive cell, where it gets processed to release a toxic warhead-a nonhydrolyzable aspartyl-adenylate, which inhibits aspartyl-trna synthetase. adenylation of peptide precursors is carried out by mccb thif-type nad/fad adenylyltransferases. here, we describe a novel microcin c-like compound from bacillus amyloliquefaciens. the b. amyloliquefaciens mccb de ...201627934031
identification of root-secreted compounds involved in the communication between cucumber, the beneficial bacillus amyloliquefaciens, and the soil-borne pathogen fusarium oxysporum.colonization of plant growth-promoting rhizobacteria (pgpr) is critical for exerting their beneficial effects on the plant. root exudation is a major factor influencing the colonization of both pgpr and soil-borne pathogens within the root system. however, the tripartite interaction of pgpr, plant roots, and soil-borne pathogens is poorly understood. we screened root exudates for signals that mediate tripartite interactions in the rhizosphere. in a split-root system, we found that root colonizat ...201727937752
malonylome analysis of rhizobacterium bacillus amyloliquefaciens fzb42 reveals involvement of lysine malonylation in polyketide synthesis and plant-bacteria interactions.using the combination of affinity enrichment and high-resolution lc-ms/ms analysis, we performed a large-scale lysine malonylation analysis in the model representative of gram-positive plant growth-promoting rhizobacteria (pgpr), bacillus amyloliquefaciens fzb42. altogether, 809 malonyllysine sites in 382 proteins were identified. the bioinformatic analysis revealed that lysine malonylation occurs on the proteins involved in a variety of biological functions including central carbon metabolism, ...201727939684
molecular dynamics and protein interaction studies of lipopeptide (iturin a) on α- amylase of spodoptera litura.the small mottled willow moth (spodoptera litura) is one of the best-known agricultural pest insects. to understand the insecticidal activity, we have selected iturin a compound produced by bacillus amyloliquefaciens rhnk22 which showed the strongest and most common inhibitory effect on the spodoptera litura protein. in this work we have identified the action of iturin a on α- amylase is a major digestive enzyme of spodoptera litura using docking studies. a 3d model of α- amylase from spodoptera ...201727940096
bacillus amyloliquefaciens sc06 alleviates the oxidative stress of ipec-1 via modulating nrf2/keap1 signaling pathway and decreasing ros production.oxidative stress (os) plays a major role in the gastrointestinal disorders. although probiotics were reported to repress os, few researches compared the antioxidant ability of different bacillus strains and deciphered the mechanisms. to select a bacillus strain with higher antioxidant capacity, we used h2o2 to induce intestinal porcine epithelial cell 1 (ipec-1) os model. the most suitable h2o2 concentration and incubation time were determined by the half lethal dose and methyl thiazolyl tetrazo ...201727957629
antibacterial compounds-macrolactin alters the soil bacterial community and abundance of the gene encoding pks.macrolactin produced by many soil microbes has been shown to be an efficient antibacterial agent against many bacterial pathogens. however, studies examining the effect of macrolactin on both the soil bacterial community and the intrinsic bacterial species that harbor genes responsible for the production of this antibiotic have not been conducted so far. in this study, a mixture of macrolactin was isolated from the liquid culture of bacillus amyloliquefaciens njn-6, and applied to the soil once ...201627965639
spore-forming bacteria responsible for food spoilage.this review explores the main spore-forming bacteria involved in the spoilage of various processed foods. bakery products are specifically spoiled by bacillus species, the dominant one being bacillus amyloliquefaciens, while different clostridium species classically contaminate refrigerated vacuum-packed meats. these two genera have also been isolated from milk products, even when milk is pasteurized, sterilized, dehydrated or fermented, according to heat treatment and storage temperature. final ...201627989764
high-level heat resistance of spores of bacillus amyloliquefaciens and bacillus licheniformis results from the presence of a spova operon in a tn1546 transposon.bacterial endospore formers can produce spores that are resistant to many food processing conditions, including heat. some spores may survive heating processes aimed at production of commercially sterile foods. recently, it was shown that a spova operon, designated spova(2mob), present on a tn1546 transposon in bacillus subtilis, leads to profoundly increased wet heat resistance of b. subtilis spores. such tn1546 transposon elements including the spova(2mob) operon were also found in several str ...201627994575
decreased formation of branched-chain short fatty acids in bacillus amyloliquefaciens by metabolic engineering.to reduce the unpleasant odor during 1-deoxynojirimycin (dnj) production, the genes of leucine dehydrogenase (bcd) and phosphate butryltransferase (ptb) were deleted from bacillus amyloliquefaciens hz-12, and the concentrations of branched-chain short fatty acids (bcfas) and dnj were compared.201727999972
design, synthesis and antimicrobial activities of thiouracil derivatives containing triazolo-thiadiazole as seca inhibitors.a series of novel thiouracil derivatives containing a triazolo-thiadiazole moiety (7a-7l) have been synthesized by structural modifications on a lead seca inhibitor, 2. all the compounds have been evaluated for their antibacterial activities against bacillus amyloliquefaciens, staphylococcus aureus, and bacillus subtilis. compounds 7d and 7g were also tested for their inhibitory activities against seca atpase due to their promising antimicrobial activities. the inhibitory activity of compound 7d ...201728039774
potential of a new strain of bacillus amyloliquefaciens buz-14 as a biocontrol agent of postharvest fruit diseases.the biocontrol potential of the bacillus amyloliquefaciens strain buz-14 was tested against the main postharvest diseases of orange, apple, grape and stone fruit. after characterizing the temperature and ph growth curves of strain buz-14, its in vitro antifungal activity was determined against botrytis cinerea, monilinia fructicola, m. laxa, penicillium digitatum, p. expansum and p. italicum. subsequently, in vivo activity was tested against these pathogens by treating fruit with cells, endospor ...201728040156
design, synthesis and antibacterial activities of thiouracil derivatives containing acyl thiourea as seca inhibitors.a series of novel thiouracil derivatives containing an acyl thiourea moiety (7a-7x) have been synthesized by structural modification of a lead seca inhibitor, 2. all the compounds have been evaluated for their antibacterial activities against bacillus amyloliquefaciens, staphylococcus aureus, and bacillus subtilis. compounds 7c, 7m, 7u, 7v exhibited promising activities against above bacteria. such four compounds were further tested for their inhibitory activity against seca atpase, and the resu ...201628041832
isotryptophan from antarctic bacillus amyloliquefaciens pc3: purification, identification, characterization, and antifungal activity.strain pc3 was isolated from antarctic seawater and identified as bacillus amyloliquefaciens. a compound with antifungal activity was purified from the fermentation supernatant of b. amyloliquefaciens pc3. its structure was determined to be (s)-2-amino-3-(1h-indol-2-yl) propanoic acid, named as isotryptophan, based on detailed analysis of its nuclear magnetic resonance (nmr) and high-resolution electrospray ionisation mass spectrometry (hr-esi-ms). isotryptophan exhibited good thermal stability ...201728044456
glycin-rich antimicrobial peptide yd1 from b. amyloliquefaciens, induced morphological alteration in and showed affinity for plasmid dna of e. coli.antimicrobial peptides (amps), low-molecular-weight proteins with broad-spectrum antimicrobial activity, are the most promising candidates for the development of novel antimicrobials. a powerful cationic glycine-rich amp yd1 (mw ~ 1.0 kda) was purified from bacillus amyloliquefaciens cbsyd1 isolated from traditional korean fermented food kimchi, for the treatment of multidrug-resistant (mdr) bacteria. strain cbsyd1 was identified 99.79% similar to bacillus amyloliquefaciens subsp. plantarum fzb4 ...201728050849
secondary metabolites from bacillus amyloliquefaciens isolated from soil can kill burkholderia pseudomallei.bacillus species are gram-positive bacteria found in abundance in nature and their secondary metabolites were found to possess various potential activities, notably antimicrobial. in this study, bacillus amyloliquefaciens n2-4 and n3-8 were isolated from soil and their metabolites could kill burkholderia pseudomallei, a gram-negative pathogenic bacterium also found in soil in its endemic areas. moreover, the metabolites were able to kill drug resistant isolates of b. pseudomallei and also inhibi ...201728050857
comparative genomic analysis of bacillus amyloliquefaciens and bacillus subtilis reveals evolutional traits for adaptation to plant-associated habitats.bacillus subtilis and its sister species b. amyloliquefaciens comprise an evolutionary compact but physiologically versatile group of bacteria that includes strains isolated from diverse habitats. many of these strains are used as plant growth-promoting rhizobacteria (pgpr) in agriculture and a plant-specialized subspecies of b. amyloliquefaciens-b. amyloliquefaciens subsp. plantarum, has recently been recognized, here we used 31 whole genomes [including two newly sequenced pgpr strains: b. amyl ...201628066362
malonylome of the plant growth promoting rhizobacterium with potent biocontrol activity, bacillus amyloliquefaciens fzb42.the data presented in this article are related to the publication entitled "malonylome analysis of rhizobacterium bacillus amyloliquefaciens fzb42 reveals involvement of lysine malonylation in polyketide synthesis and plant-bacteria interactions"(doi:10.1016/j.jprot.2016.11.022) (b. fan, y. li, l. li et al.) [1]. this article presented the raw information of all malonyllysine sites identified by lc-ms/ms in the bacillus amyloliquefaciens fzb42. further, the functional features and conservation o ...201728070544
the antibacterial activity and mechanism of ginkgolic acid c15:1.the present study investigated the antibacterial activity and underlying mechanisms of ginkgolic acid (ga) c15:1 monomer using green fluorescent protein (gfp)-labeled bacteria strains.201728088196
isolation and identification of phyllospheric bacteria possessing antimicrobial activity from astragalus obtusifolius, prosopis juliflora, xanthium strumarium and hippocrepis unisiliqousa.the widespread utilization of antimicrobial compounds has caused emergence of resistant microorganisms in the world. hence, the research to probe the products with antimicrobial features has led to finding natural habitats and discovering new pharmaceutical products.201728090278
bacillus volatiles adversely affect the physiology and ultra-structure of ralstonia solanacearum and induce systemic resistance in tobacco against bacterial wilt.volatile organic compounds (vocs) produced by various bacteria have significant potential to enhance plant growth and to control phytopathogens. six of the most effective antagonistic bacillus spp. were used in this study against ralstonia solanacearum (rsc) tbbs1, the causal agent of bacterial wilt disease in tobacco. bacillus amyloliquefaciens fzb42 and bacillus artrophaeus lssc22 had the strongest inhibitory effect against rsc. thirteen vocs produced by fzb42 and 10 by lssc22 were identified ...201728091587
adaptation of primocane fruiting raspberry plants to environmental factors under the influence of bacillus strains in western siberia.in geographical locations with a short vegetative season and continental climate that include western siberia, growing primocane fruiting raspberry varieties becomes very important. however, it is necessary to help the plants to overcome the environmental stress factors. this study aimed to evaluate the impact of the pre-planting treatment of primocane fruiting raspberry root system with bacillus strains on the following plant development under variable environmental conditions. in 2012, bacillu ...201728092002
combinatorial effect of bacillus amyloliquefaciens ag1 biosurfactant and bacillus thuringiensis vip3aa16 toxin on spodoptera littoralis larvae.spodoptera littoralis, one of the most serious and destructive agricultural pests in the world, is very susceptible to vip3 toxin. in order to develop a new efficient bioinsecticide and to prevent the development of resistance by the target pest, insecticidal activity of biosurfactant produced by bacillus amyloliquefaciens ag1 was evaluated against s. littoralis. bioassays revealed the susceptibility of the first instar larvae of this pest to ag1 biosurfactant with an lc50 of 245ng/cm(2). moreov ...201728093209
genome and transcriptome analysis of surfactin biosynthesis in bacillus amyloliquefaciens mt45.natural bacillus isolates generate limited amounts of surfactin (<10% of their biomass), which functions as an antibiotic or signalling molecule in inter-/intra-specific interactions. however, overproduction of surfactin in bacillus amyloliquefaciens mt45 was observed at a titre of 2.93 g/l, which is equivalent to half of the maximum biomass. to systemically unravel this efficient biosynthetic process, the genome and transcriptome of this bacterium were compared with those of b. amyloliquefacien ...201728112210
heterologous expression and enzymatic characterization of γ-glutamyltranspeptidase from bacillus amyloliquefaciens.γ-glutamyltranspeptidase (ggt) catalyzes the cleavage of γ-glutamyl compounds and the transfer of γ-glutamyl moiety to water or to amino acid/peptide acceptors. ggt can be utilized for the generation of γ-glutamyl peptides or glutamic acid, which are used as food taste enhancers. in the present study, bacillus amyloliquefaciens smb469 with high ggt activity was isolated from doenjang, a traditional fermented soy food of korea. the gene encoding ggt from b. amyloliquefaciens smb469 (baggt469) was ...201728120195
the antibacterial activity of e. coli bacteriophage lysin lysep3 is enhanced by fusing the bacillus amyloliquefaciens bacteriophage endolysin binding domain d8 to the c-terminal region.bacteriophage endolysin is one of the most promising antibiotic substitutes, but in gram-negative bacteria, the outer membrane prevents the lysin from hydrolyzing peptidoglycans and blocks the development of lysin applications. the prime strategy for new antibiotic substitutes is allowing lysin to access the peptidoglycan from outside of the bacteria by reformation of the lysin. in this study, the novel escherichia coli (e. coli) phage lyase lysep3, which lacks outside-in catalytic ability, was ...201728124780
production and characterization of organic solvent-tolerant cellulase from bacillus amyloliquefaciens ak9 isolated from hot spring.a cellulase-producing bacterium, designated as strain ak9, was isolated from a hot spring of tatta pani, azad kashmir, pakistan. the bacterium was identified as bacillus amyloliquefaciens through 16s rrna sequencing. cellulase from strain ak9 was able to liberate glucose from soluble cellulose and carboxymethyl cellulose (cmc). enzyme was purified through size exclusion chromatography and a single band of ∼47 kda was observed on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page ...201728130767
acetylation and deacetylation for sucralose preparation by a newly isolated bacillus amyloliquefaciens wzs01.sucralose is a non-nutritive artificial sweetener used in a broad range of foods and beverages. in the present study, bacillus amyloliquefaciens wzs01 was isolated, identified, and used as a catalyst both in regioselective acylation and deacetylation for sucralose preparation. bacterial cells were immobilized on polyurethane foam and utilized to synthesize sucrose-6-acetate regioselectively. the yield of sucrose-6-acetate was >95% with 60 mm sucrose after 22 h of reaction. free cells could hydro ...201728131539
key impact of an uncommon plasmid on bacillus amyloliquefaciens subsp. plantarum s499 developmental traits and lipopeptide production.the rhizobacterium bacillus amyloliquefaciens subsp. plantarum s499 (s499) is particularly efficient in terms of the production of cyclic lipopeptides, which are responsible for the high level of plant disease protection provided by this strain. sequencing of the s499 genome has highlighted genetic differences and similarities with the closely related rhizobacterium b. amyloliquefaciens subsp. plantarum fzb42 (fzb42). more specifically, a rare 8008 bp plasmid (ps499) harboring a rap-phr cassette ...201728154555
bacillus amyloliquefaciens, bacillus velezensis, and bacillus siamensis form an "operational group b. amyloliquefaciens" within the b. subtilis species complex.the plant growth promoting model bacterium fzb42(t) was proposed as the type strain of bacillus amyloliquefaciens subsp. plantarum (borriss et al., 2011), but has been recently recognized as being synonymous to bacillus velezensis due to phylogenomic analysis (dunlap c. et al., 2016). however, until now, majority of publications consider plant-associated close relatives of fzb42 still as "b. amyloliquefaciens." here, we reinvestigated the taxonomic status of fzb42 and related strains in its cont ...201728163698
genomic and metabolic traits endow bacillus velezensis cc09 with a potential biocontrol agent in control of wheat powdery mildew disease.bacillus velezensis cc09, which was isolated from healthy leaves of cinnamomum camphora and previously identified as bacillus amyloliquefaciens cc09, shows great potential as a new biocontrol agent, in control of many phytopathogenic diseases. to extend our understanding of the potential antifungal capacities, we did a whole genome analysis of strain cc09. result shows that strain cc09 has a relatively large genome size (4.17mb) with an average gc content of 46.1%, and 4021 predicted genes. thir ...201728164794
application of bacteria from non-cultivated plants to promote growth, alter root architecture and alleviate salt stress of cotton.cotton seeds are frequently treated with acid to remove fibres and reduce seed-transmitted diseases. this process also eliminates beneficial bacteria on the seed surface. the goal of this research was to seek and apply beneficial bacteria to acid delinted cotton seeds to evaluate their growth-promoting and salt stress alleviating effects in seedlings.201728176460
loap is a broadly conserved antiterminator protein that regulates antibiotic gene clusters in bacillus amyloliquefaciens.a valuable resource available in the search for new natural products is the diverse microbial life that spans the planet. a large subset of these microorganisms synthesize complex specialized metabolites exhibiting biomedically important activities. a limiting step to the characterization of these compounds is an elucidation of the genetic regulatory mechanisms that oversee their production. although proteins that control transcription initiation of specialized metabolite gene clusters have been ...201728191883
bacillus spp. isolated from puba as a source of biosurfactants and antimicrobial lipopeptides.several products of industrial interest are produced by bacillus, including enzymes, antibiotics, amino acids, insecticides, biosurfactants and bacteriocins. this study aimed to investigate the potential of two bacterial isolates (p5 and c3) from puba, a regional fermentation product from cassava, to produce multiple substances with antimicrobial and surface active properties. phylogenetic analyses showed close relation of isolates p5 and c3 with bacillus amyloliquefaciens and bacillus thuringie ...201728197131
improvement of levan production in bacillus amyloliquefaciens through metabolic optimization of regulatory elements.levan is a functional homopolymer of fructose with considerable applications in food, pharmaceutical and cosmetic industries. to improve the levan production in bacillus amyloliquefaciens, the regulatory elements of sacb (encoding levansucrase) expression and levansucrase secretion were optimized. four heterologous promoters were evaluated for sacb expression, and the pgrac promoter led to the highest level for both sacb transcription and levansucrase enzyme activity. the levan production in the ...201728197690
selection of bacillus species for targeted in situ release of prebiotic galacto-rhamnogalacturonan from potato pulp in piglets.we have previously shown that galacto-rhamnogalacturonan fibers can be enzymatically extracted from potato pulp and that these fibers have potential for exerting a prebiotic effect in piglets. the spore-forming bacillus species are widely used as probiotics in feed supplements for pigs. in this study, we evaluated the option for further functionalizing bacillus feed supplements by selecting strains possessing the enzymes required for extraction of the potentially prebiotic fibers. we established ...201728204884
antifungal effect of volatile organic compounds produced by bacillus amyloliquefaciens cpa-8 against fruit pathogen decays of cherry.the present work focuses on the antifungal effect of volatile organic compounds (vocs) produced by bacillus amyloliquefaciens cpa-8 against monilinia laxa, m. fructicola and botrytis cinera, three postharvest fruit pathogens of sweet cherry fruit. vocs were evaluated with a double petri dish assay against mycelial and colony growth of target pathogens. for this purpose, cpa-8 was grown on different media and cultured for 24 and 48 h at 30 °c before assays. data showed that mycelial growth inhibi ...201728213029
high-level extracellular protein production in bacillus subtilis using an optimized dual-promoter expression system.we recently constructed a bacillus subtilis strain (cctcc m 2016536) from which we had deleted the srfc, spoiiac, npre, apre and amye genes. this strain is capable of robust recombinant protein production and amenable to high-cell-density fermentation. because the promoter is among the factors that influence the production of target proteins, optimization of the initial promoter, pamyq from bacillus amyloliquefaciens, should improve protein expression using this strain. this study was undertaken ...201728219382
combinatorial effect of mutagenesis and medium component optimization on bacillus amyloliquefaciens antifungal activity and efficacy in eradicating botrytis cinerea.this work is directed towards bacillus amyloliquefaciens strain blb371 metabolite production for biocontrol of fungal phytopathogens. in order to maximise antifungal metabolite production by this strain, two approaches were combined: random mutagenesis and medium component optimization. after three rounds of mutagenesis, a hyper active mutant, named m3-7, was obtained. it produces 7 fold more antifungal metabolites (1800au/ml) than the wild strain in mc medium. a hybrid design was applied to opt ...201728219523
beneficial effects of fermented sardinelle protein hydrolysates on hypercaloric diet induced hyperglycemia, oxidative stress and deterioration of kidney function in wistar rats.this study investigated the potential effects of fermented sardinelle protein hydrolysates (fsphs) obtained by two proteolytic bacteria, bacillus subtilis a26 (fsph-a26) and bacillus amyloliquefaciens an6 (fsph-an6), on hypercaloric diet (hcd) induced hyperglycemia and oxidative stress in rats. effects of fsphs on blood glucose level, glucose tolerance, α-amylase activity and hepatic glycogen content were investigated, as well as their effect on the oxidative stress state. biochemical findings r ...201728242930
effects of probiotic bacillus as a substitute for antibiotics on antioxidant capacity and intestinal autophagy of piglets.the objective of this study was to evaluate effects of probiotic bacillus amyloliquefaciens (ba) as a substitute for antibiotics on growth performance, antioxidant ability and intestinal autophagy of piglets. ninety piglets were divided into three groups: g1 (containing 150 mg/kg aureomycin in the diet); g2 (containing 75 mg/kg aureomycin and 1 × 10(8) cfu/kg ba in the diet); g3 (containing 2 × 10(8) cfu/kg ba in the diet without any antibiotics). each treatment had three replications of ten pig ...201728244029
beneficial rhizobacterium bacillus amyloliquefaciens sqr9 induces plant salt tolerance through spermidine production.the inoculation of plants with plant growth-promoting rhizobacterium (pgpr) has been an effective strategy for enhancing plant salt tolerance to diminish the loss of agricultural productivity caused by salt stress, but the signal transmitted from bacteria to the plant under salt stress is poorly understood. in this study, the salt tolerance of arabidopsis thaliana and zea mays was enhanced by inoculation with bacillus amyloliquefaciens sqr9. using dialysis bags with different molecular weight cu ...201728291380
plant growth-promoting endophytic bacteria versus pathogenic infections: an example of bacillus amyloliquefaciens rwl-1 and fusarium oxysporum f. sp. lycopersici in tomato.fungal pathogenic attacks are one of the major threats to the growth and productivity of crop plants. currently, instead of synthetic fungicides, the use of plant growth-promoting bacterial endophytes has been considered intriguingly eco-friendly in nature. here, we aimed to investigate the in vitro and in vivo antagonistic approach by using seed-borne endophytic bacillus amyloliquefaciens rwl-1 against pathogenic fusarium oxysporum f. sp. lycopersici. the results revealed significant suppressio ...201728321368
an approach to low-density polyethylene biodegradation by bacillus amyloliquefaciens.low-density polyethylene (ldpe) is a major cause of persistent and long-term environmental pollution. in this paper, two bacterial isolates bacillus amyloliquefaciens (bsm-1) and bacillus amyloliquefaciens (bsm-2) were isolated from municipal solid soil and used for polymer degradation studies. the microbial degradation ldpe was analyzed by dry weight reduction of ldpe film, change in ph of culture media, co2 estimation, scanning electron microscopy (sem), and fourier transform infrared ftir spe ...201528324364
optimization of carboxymethylcellulase production from bacillus amyloliquefaciens ss35.in this paper, we have attempted optimization of production of enzyme carboxymethylcellulase or endoglucanase from the bacterium bacillus amyloliquefaciens ss35. optimization has been carried out in two stages using statistical experimental design, viz. medium optimization and optimization of fermentation parameters. for medium optimization, plackett-burman design followed by central composite design (ccd) was used, while for optimization of fermentation parameters one-variable-at-a-time method ...201428324477
biosurfactant production from marine bacteria associated with sponge callyspongia diffusa.marine-derived biosurfactants have gained significant attention due to their structural and functional diversity. biosurfactant production was performed using bacteria associated with callyspongia diffusa, a marine sponge inhabiting the southern coast of india. a total of 101 sponge-associated bacteria were isolated on different media, of which 29 isolates showed positive result for biosurfactant production. among the 29 positive isolates, four were selected based on highest emusification activi ...201528324546
a statistical approach for the production of thermostable and alklophilic alpha-amylase from bacillus amyloliquefaciens kcp2 under solid-state fermentation.the bacterial strain producing thermostable, alklophilic alpha-amylase was identified as bacillus amyloliquefaciens kcp2 using 16s rdna gene sequencing data (ncbi accession no: kf112071). medium components were optimized through the statistical approach for the synthesis of alpha-amylase by the organism under solid-state fermentation using wheat bran as the substrate. the medium components influencing the enzyme production were identified using a two-level fractional factorial plackett-burman de ...201528324580
immobilization of bacillus amyloliquefaciens sp1 and its alkaline protease in various matrices for effective hydrolysis of casein.an extracellular alkaline protease producing b. amyloliquefaciens sp1 was isolated from apple rhizosphere having multifarious plant growth-promoting activities. b. amyloliquefaciens sp1 protease was immobilized using various concentrations of calcium alginate, agar and polyacrylamide to determine the optimum concentration for formation of the beads. enzyme activity before immobilization (at 60 °c, ph 8.0 for 5 min) was 3580 µg/ml/min. the results of immobilization with various matrices revealed ...201628330279
production of exopolysaccharides by selected bacillus strains: optimization of media composition to maximize the yield and structural characterization.selected bacillus strains were investigated for the production of exopolysaccharides (eps) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (m1), (ii) succinate-containing mineral base-medium with added yeast extract (m2) and (iii) tryptone and yeast extract-containing base-medium (m3). modest eps yield of 6.7g/l was recorded for bacillus amyloliquefaciens 23350 grown on m1, where the eps produced was characterized by a low mw (<5kda) and of bein ...201728363654
production of surfactin from waste distillers' grains by co-culture fermentation of two bacillus amyloliquefaciens strains.distillers' grains (dgs), the main waste by-products of chinese liquor industry, were used as substrate for surfactin production. bacillus amyloliquefaciens mt45 could grow with dgs as sole carbon source to produce 1.04g/l surfactin. however, low amylase activity of mt45 limited sugar supply and the subsequent surfactin production. therefore, mt45 was co-cultured with bacillus strains that exhibited remarkable hydrolases activities. surfactin yield increased by 50% when mt45 was co-cultured with ...201728365354
intramembrane protease rasp boosts protein production in bacillus.the microbial cell factory bacillus subtilis is a popular industrial platform for high-level production of secreted technical enzymes. nonetheless, the effective secretion of particular heterologous enzymes remains challenging. over the past decades various studies have tackled this problem, and major improvements were achieved by optimizing signal peptides or removing proteases involved in product degradation. on the other hand, serious bottlenecks in the protein export process per se remained ...201728376795
probiotic bacillus amyloliquefaciens sc06 induces autophagy to protect against pathogens in macrophages.probiotics are increasingly applied in popularity in both humans and animals. decades of research has revealed their beneficial effects, including the immune modulation in intestinal pathogens inhibition. autophagy-a cellular process that involves the delivery of cytoplasmic proteins and organelles to the lysosome for degradation and recirculation-is essential to protect cells against bacterial pathogens. however, the mechanism of probiotics-mediated autophagy and its role in the elimination of ...201728382029
production of bioactive hydrolysate using protease, β-glucosidase and α-amylase of bacillus spp. isolated from kinema.the aim of this study was the production of soybean bioactive hydrolysate using bacillus spp. isolated from kinema. totally 251 bacteria isolated from kinema samples, collected at different time period were screened for protease, β-glucosidase and α-amylase activities and further identified by ardra based grouping followed by analysis of 16s rrna gene sequence similarity. the results showed that bacillus subtilis, bacillus amyloliquefaciens and bacillus licheniformis were the major bacillus spec ...201728384588
purification, characterization and thermostability improvement of xylanase from bacillus amyloliquefaciens and its application in pre-bleaching of kraft pulp.xylanases have important industrial applications but are most extensively utilized in the pulp and paper industry as a pre-bleaching agent. we characterized a xylanase from bacillus amyloliquefaciens strain sk-3 and studied it for kraft pulp bleaching. the purified enzyme had a molecular weight of ~50 kda with optimal activity at ph 9.0 and 50 °c. the enzyme showed good activity retention (85%) after 2 h incubation at 50 °c and ph 9.0. this enzyme obeyed michaelis-menten kinetics with regard to ...201728401458
contribution of native phosphorous-solubilizing bacteria of acid soils on phosphorous acquisition in peanut (arachis hypogaea l.).the present investigation analyzes the in vitro p solubilization [ca-p, al-p, fe(ii)-p, and fe(iii)-p] efficiency of native psb strains from acid soils of odisha and exploitation of the same through biofertilization in peanut (arachis hypogaea l.) growth and p acquisition. one hundred six numbers of soil samples with ph ≤ 5.50 were collected from five districts of odisha viz., balasore, cuttack, khordha, keonjhar, and mayurbhanj. one bacterial isolate from each district were selected and analyze ...201728455550
effect of diurnal photosynthetic activity on the fine structure of amylopectin from normal and waxy barley starch.the impact of diurnal photosynthetic activity on the fine structure of the amylopectin fraction of starch synthesized by normal barley (nbs) and waxy barley (wbs), the latter completely devoid of amylose biosynthesis, was determined following the cultivation under normal diurnal or constant light growing conditions. the amylopectin fine structures were analysed by characterizing its unit chain length profiles after enzymatic debranching as well as its φ,β-limit dextrins and its clusters and buil ...201728457958
a peptidomic approach for the identification of antioxidant and ace-inhibitory peptides in sardinelle protein hydrolysates fermented by bacillus subtilis a26 and bacillus amyloliquefaciens an6.antioxidant and angiotensin i-converting enzyme (ace)-inhibitory activities of sardinelle (sardinella aurita) protein hydrolysates (sphs) obtained by fermentation with bacillus subtilis a26 (sph-a26) and bacillus amyloliquefaciens an6 (sph-an6) were investigated. both hydrolysates showed dose-dependent antioxidant activities evaluated using various in vitro antioxidant assays. further, they were found to exhibit ace-inhibitory activity. peptides from sph-a26 and sph-an6 were analyzed by nesi-lc- ...201628460924
paraprobiotic preparation from bacillus amyloliquefaciens fptb16 modulates immune response and immune relevant gene expression in catla catla (hamilton, 1822).the present study evaluated the paraprobiotic effect of heat-inactivated bacillus amyloliquefaciens fptb16 on immunological response and immune relevant gene expression in catla (catla catla). heat inactivation of viable cells of b. amyloliquefaciens was done at 60 °c for 2 h. for preparation of paraprobiotic supplemented diet, the heat-inactivated bacteria were added to the basal diet (control) at three different inclusion levels i.e., 10(7), 10(8) and 10(9) cells g(-1) diet. fish (25.98 ± 2.57 ...201728476667
functional silver nanocomposites as broad-spectrum antimicrobial and biofilm-disrupting agents.biofilms' tolerance has become a serious clinical concern due to their formidable resistance to conventional antibiotics and prevalent virulence. therefore, there is an urgent need to develop alternative antimicrobial agents to eradicate biofilms but avoid using antibiotics. herein, we successfully developed polymer functional silver nanocomposites by reduction of silver nitrate in the presence of a biocompatible carbohydrate polymer and a membrane-disrupting cationic polymer. the nanocomposites ...201728481506
biocontrol of gray mold decay in pear by bacillus amyloliquefaciens strain ba3 and its effect on postharvest quality parameters.the economic losses caused by postharvest fruits diseases have attracted global attention. traditional chemical fungicide could not meet the need of humans. in recent years, microbial agent which has begun to take the place of chemical fungicide comes into people’s vision. the aim of this paper was to investigate the potential of bacillus amyloliquefaciens strain ba3 for its biocontrol capability on gray mold decay of pears and its effect on postharvest quality of pears. compared with other trea ...201628517918
development of jerusalem artichoke resource for efficient one-step fermentation of poly-(γ-glutamic acid) using a novel strain bacillus amyloliquefaciens nx-2s.this study aimed to develop non-food fermentation for the cost-effective production of poly-(γ-glutamic acid) (γ-pga) using a novel strain of bacillus amyloliquefaciens nx-2s. the new isolate assimilated inulin more efficiently than other carbohydrates from jerusalem artichoke, without hydrolytic treatment. to investigate the effect of inulin on γ-pga production, the transcript levels of γ-pga synthetase genes (pgsb, pgsc, pgsa), regulatory genes (coma, degq, degs), and the glutamic acid biosynt ...201728521229
molecular detection by analysis of the 16s rrna gene of fecal coliform bacteria from the two korean apodemus species (apodemus agrarius and a. peninsulae).wild mouse feces can disseminate zoonotic microorganisms throughout a farm, which is a great threat to human health and can lead to economic loss through contaminated agricultural produce. to assess the microbial communities, especially fecal coliform bacteria, we used two methods. first, we isolated bacterial colonies onto the common media lb (lactose broth) agar, tsa (tryptic soy agar), and mrs (de man, rogosa, and sharpe) agar, and then randomly select colonies from each plate and stocked the ...201728525654
efficacy of bacillus subtilis and bacillus amyloliquefaciens in the control of aspergillus parasiticus growth and aflatoxins production on pistachio.pistachio (pistacia vera) is an important nut for its economic, nutritional and health aspects but it can be contaminated by aflatoxigenic fungi in the field and during storage. biological control could be considered as an alternative to chemical treatment. in this study, we evaluated the antifungal and anti-mycotoxigenic capability of two bacillus spp. both in vitro and on pistachio kernels. in in vitro conditions, both strains were able to reduce the mycelial growth and they were able to degra ...201728531554
enhancing poly-γ-glutamic acid production in bacillus amyloliquefaciens by introducing the glutamate synthesis features from corynebacterium glutamicum.poly-γ-glutamic acid (γ-pga) is a valuable polymer with glutamate as its sole precursor. enhancement of the intracellular glutamate synthesis is a very important strategy for the improvement of γ-pga production, especially for those glutamate-independent γ-pga producing strains. corynebacterium glutamicum has long been used for industrial glutamate production and it exhibits some unique features for glutamate synthesis; therefore introduction of these metabolic characters into the γ-pga producin ...201728532451
identification of diacetonamine from soybean curd residue as a sporulation-inducing factor toward bacillus spp.under bioassay-guided investigation, a sporulation-inducing factor (sif) toward bacillus spp. was searched for in methanol (meoh) extracts of soybean curd residues, and diacetonamine (1) was identified as the active compound. sif was first isolated as a monoacetylated derivative (2, 4.1 mg from 655 g soybean curd residues), and its chemical structure was elucidated by field desorption mass spectrometry, electron ionization mass spectrometry, and nuclear magnetic resonance (nmr) analyses. after 4 ...201728545259
stimulation of fengycin-type antifungal lipopeptides in bacillus amyloliquefaciens in the presence of the maize fungal pathogen rhizomucor variabilis.most isolates belonging to the bacillus amyloliquefaciens subsp. plantarum clade retain the potential to produce a vast array of structurally diverse antimicrobial compounds that largely contribute to their efficacy as biocontrol agents against numerous plant fungal pathogens. in that context, the role of cyclic lipopeptides (clps) has been well-documented but still little is known about the impact of interactions with other soil-inhabiting microbes on the expression of these molecules. in this ...201728555132
importance of prumycin produced by bacillus amyloliquefaciens sd-32 in biocontrol against cucumber powdery mildew disease.powdery mildew disease of cucurbits is caused mainly by podosphaera fusca, which is one of the most important limiting factors in cucurbit production worldwide. previously we reported that bacillus amyloliquefaciens biocontrol strain sd-32 produces c17 bacillomycin d and [ile]surfactin, and that these metabolites play important roles in sd-32's biocontrol over cucumber gray mold disease. our further investigation demonstrated that the culture broth and its supernatant suppressed cucumber powdery ...201728560847
characterisation and antimicrobial activity of biosurfactant extracts produced by bacillus amyloliquefaciens and pseudomonas aeruginosa isolated from a wastewater treatment plant.biosurfactants are unique secondary metabolites, synthesised non-ribosomally by certain bacteria, fungi and yeast, with their most promising applications as antimicrobial agents and surfactants in the medical and food industries. naturally produced glycolipids and lipopeptides are found as a mixture of congeners, which increases their antimicrobial potency. sensitive analysis techniques, such as liquid chromatography coupled to mass spectrometry, enable the fingerprinting of different biosurfact ...201728571306
variants of lipopeptides and glycolipids produced by bacillus amyloliquefaciens and pseudomonas aeruginosa cultured in different carbon substrates.the quantitative and qualitative effect of water immiscible and miscible carbon-rich substrates on the production of biosurfactants, surfactin and rhamnolipids, by bacillus amyloliquefaciens st34 and pseudomonas aeruginosa st5, respectively, was analysed. a small-scale high throughput 96 deep-well micro-culture method was utilised to cultivate the two strains in mineral salt medium (msm) supplemented with the water miscible (glucose, glycerol, fructose and sucrose) and water immiscible carbon so ...201728571307
impact of mannanase-producing bacillus spp. on the accuracy of the 3m petrifilm aerobic count method.consistent deviations of the 3m petrifilm aerobic counts (ac) from the standard pour plate aerobic plate count (apc) were observed with dehydrated onion and garlic products. a large study was designed to determine the relationship of these two methods and the root cause for the deviations. a total of 3,800 dehydrated onion and garlic samples were analyzed by both the petrifilm ac and the standard pour plate apc method. large spreader-like liquefied areas were observed on numerous petrifilm plate ...201728574306
evaluating the feasibility of fermentation starter inoculated with bacillus amyloliquefaciens for improving acetoin and tetramethylpyrazine in baoning bran vinegar.fermentation starters (daqu) used in present study included traditional herb daqu (c daqu), modified daqu without herbs (m daqu) and s daqu fermented by inoculating acetoin and tetramethylpyrazine high-producing bacterium bacillus amyloliquefaciens into m daqu. to evaluate the feasibility of s daqu combined with m daqu applied for improving contents of acetoin and tetramethylpyrazine in baoning bran vinegar without remarkably changing the original microbial community and the other volatiles cont ...201728578227
addition of plant-growth-promoting bacillus subtilis pts-394 on tomato rhizosphere has no durable impact on composition of root microbiome.representatives of the genus bacillus are increasingly used in agriculture to promote plant growth and to protect against plant pathogens. unfortunately, hitherto the impact of bacillus inoculants on the indigenous plant microbiota has been investigated exclusively for the species bacillus amyloliquefaciens and was limited to prokaryotes, whilst eukaryotic member of this community, e.g. fungi, were not considered.201728583081
menaquinone-7 production from maize meal hydrolysate by bacillus isolates with diphenylamine and analogue resistance.a menaquinone-7 (mk-7) high-producing strain needs to be isolated to increase mk-7 production, in order to meet a requirement of mk-7 given the low mk-7 content in food products. this article focuses on developing mk-7 high-producing strains via screening and mutagenesis by an atmospheric and room temperature plasma (artp) mutation breeding system. we isolated an mk-7-producing strain y-2 and identified it as bacillus amyloliquefaciens, which produced (7.1±0.5) mg/l of mk-7 with maize meal hydro ...201728585422
construction of energy-conserving sucrose utilization pathways for improving poly-γ-glutamic acid production in bacillus amyloliquefaciens.sucrose is an naturally abundant and easily fermentable feedstock for various biochemical production processes. by now, several sucrose utilization pathways have been identified and characterized. among them, the pathway consists of sucrose permease and sucrose phosphorylase is an energy-conserving sucrose utilization pathway because it consumes less atp when comparing to other known pathways. bacillus amyloliquefaciens nk-1 strain can use sucrose as the feedstock to produce poly-γ-glutamic acid ...201728587617
the profile and antimicrobial activity of bacillus lipopeptide extracts of five potential biocontrol strains.in this study the efficacy of two different methods for extracting lipopeptides produced by five bacillus strains-ethyl acetate extraction, and acid precipitation followed by methanol extraction-was investigated using mass spectrometry. high performance thin layer chromatography (hptlc) was also used for the simultaneous separation of complex mixtures of lipopeptide extracts and for the determination of antimicrobial activity of their components. the mass spectra clearly showed well-resolved gro ...201728588570
efficacy of bacillus amyloliquefaciens as biocontrol agent to fight fungal diseases of maize under tropical climates: from lab to field assays in south kivu.in the province of south kivu (democratic republic of congo), warm and humid climatic conditions favor the development and spreading of phytopathogens. the resulting diseases cause important losses in production both in crop and after harvest. in this study, we wanted to evaluate the potential of bacillus amyloliquefaciens as biocontrol agent to fight some newly isolated endemic fungal pathogens infesting maize. the strain s499 has been selected based on its high in vitro antagonistic activity c ...201728600796
microbial interactions for enhancement of α-amylase production by bacillus amyloliquefaciens 04bba15 and lactobacillus fermentum 04bba19.interactions occurring between saccharomyces cerevisiae and two thermostable α-amylase producing strains (bacillus amyloliquefaciens 04bba15 and lactobacillus fermentum 04bba19) were analyzed by comparing their growth patterns obtained in isolation with those obtained in mixture. the difference between the patterns was assessed using analysis of variance (anova) in order to measure how much the growth of an organism was affected by other. the results showed two types of interactions in mixed cul ...201428626668
functional enrichment of mannanase-treated spent brewer yeast.in this study, the effect of bacillus amyloliquefaciens-produced β-mannanase on the nutrient diffusion (release) and antioxidant activity of spent brewer yeast (sby) was investigated. three pretreatments were performed: (1) autolysis at 50°c for 24 h; (2) autolysis at 50°c for 24 h, with the addition of β-mannanase during the autolysis; (3) autolysis at 50°c for 24 h, and the β-mannanase was added for another 12 h treatment. the pretreatments with the addition of β-mannanase caused significant c ...201728636439
isolation of a potential biocontrol agent paenibacillus polymyxa nsy50 from vinegar waste compost and its induction of host defense responses against fusarium wilt of cucumber.fusarium wilt caused by fusarium oxysporum f. sp. cucumerinum (foc) is one of the major destructive soil-borne diseases infecting cucumber. in this study, we screened 60 target strains isolated from vinegar waste compost, from which 10 antagonistic strains were identified to have the disease suppression capacity of bio-control agents. the 16s rdna gene demonstrated that the biocontrol agents were paenibacillus polymyxa (p. polymyxa), bacillus amyloliquefaciens (b. amyloliquefaciens) and bacillus ...201728647117
bacillus amyloliquefaciens say09 increases cadmium resistance in plants by activation of auxin-mediated signaling pathways.without physical contact with plants, certain plant growth-promoting rhizobacteria (pgpr) can release volatile organic compounds (vocs) to regulate nutrient acquisition and induce systemic immunity in plants. however, whether the pgpr-emitted vocs can induce cadmium (cd) tolerance of plants and the underlying mechanisms remain elusive. in this study, we probed the effects of bacillus amyloliquefaciens (strain say09)-emitted vocs on the growth of arabidopsis plants under cd stress. say09 exposure ...201728657581
biological characterization of the biocontrol agent bacillus amyloliquefaciens cpa-8: the effect of temperature, ph and water activity on growth, susceptibility to antibiotics and detection of enterotoxic genes.this work focuses on the biological understanding of the biocontrol agent bacillus amyloliquefaciens cpa-8 in order to accomplish the characterization required in the registration process for the development of a microorganism-based product. the tolerance of cpa-8 to grow under different ph-temperature and water activity (a w)-temperature conditions was widely demonstrated. regarding the ph results, optimum growth at the evaluated conditions was observed at 37 °c and ph between 7 and 5. on the c ...201728664219
strain and plastic composite support (pcs) selection for vitamin k (menaquinone-7) production in biofilm reactors.menaquinone-7 (mk-7), a subtype of vitamin k, has received a significant attention due to its effect on improving bone and cardiovascular health. current fermentation strategies, which involve static fermentation without aeration or agitation, are associated with low productivity and scale-up issues and hardly justify the commercial production needs of this vitamin. previous studies indicate that static fermentation is associated with pellicle and biofilm formations, which are critical for mk-7 ...201728667350
probiotic-mediated blueberry (vaccinium corymbosum l.) fruit fermentation to yield functionalized products for augmented antibacterial and antioxidant activity.the aim of this study was to investigate the fermentation of blueberry fruit with selected probiotic bacteria (bacillus amyloliquefaciens and lactobacillus brevis) and yeast (starmerella bombicola) isolated from fermented starfish for the extraction of functionalized products for biomedical applications. all probiotic-based fermented extracts showed augmented antibacterial and antioxidant activity compared to the control. biochemical parameters of viable cell count, titratable acidity, total phe ...201728690159
sequence comparison of phor, gyrb, groel, and chea genes as phylogenetic markers for distinguishing bacillus amyloliquefaciens and b. subtilis and for identifying bacillus strain b29.given the close genetic relationship between bacillus amyloliquefaciens and b. subtilis, distinguishing the two solely based on their physiological and biochemical characteristics and 16s rrna sequences is difficult. molecular identification was used to discover suitable genes for distinguishing the two bacteria, and to identify the bio-controlling strain b29, due to molecular identification has been paid more and more attention. the similarity of four genes, chea, gyrb, groel and phor, of the t ...201728719340
mutagenesis and characterization of a bacillus amyloliquefaciens strain for cinnamomum camphora seed kernel oil extraction by aqueous enzymatic method.the purpose of the present study was to increase the proteinase activity of the strain ncu116 by combining ultraviolet irradiation and n-methyl-n'-nitro-n-nitroso guanidine treatment, in order to enhance the efficiency of cinnamomum camphora seed kernel oil (ccsko) extraction by aqueous enzymatic method (aem). the mutated strain, designated as ncu116-1, was screened out by the ratio of hydrolytic zone diameter to colony diameter on skim milk plate. the proteinase activity (9116.1 u/ml) of ncu116 ...201728724263
recombinant bacillus amyloliquefaciens xylanase a expressed in pichia pastoris and generation of xylooligosaccharides from xylans and wheat bran.in this study, baxa (genbank: km624029), which encodes the bacillus amyloliquefaciens xylanase a (baxa), was highly expressed in pichia pastoris gs115 under the control of the aox1 promoter. the recombinant xylanase, namely repbaxa, was purified to homogeneity by using ni-affinity resin and its molecular weight was 35.0kda. the optimum temperature and ph of repbaxa were 50°c and 5.0, respectively. the kinetic parameters michaelis-menten constant (km) and maximum reaction rate (vmax) of repbaxa w ...201728732724
bacillomycin d produced by bacillus amyloliquefaciens is involved in the antagonistic interaction with the plant pathogenic fungus fusarium graminearum.fusarium graminearum (teleomorph: ascomycota, hypocreales, gibberella, gibberella zeae) is a destructive fungal pathogen that threatens the production and quality of wheat and barley worldwide. controlling this toxin-producing pathogen is a significant challenge. in the present study, the commercially available strain bacillus amyloliquefaciens (bacteria, firmicutes, bacillales, bacillus) fzb42 showed strong activity against f. graminearum the lipopeptide, bacillomycin d, produced by fzb42, was ...201728733288
assessment of the potential of the multi-enzyme producer bacillus amyloliquefaciens us573 as alternative feed additive.recently, probiotics have increasingly been used as feed additives in poultry diets as an alternative to antibiotic growth promoters fostering resistance development.201728741666
evaluation of in vivo probiotic efficiency of bacillus amyloliquefaciens in labeo rohita challenged by pathogenic strain of aeromonas hydrophila mtcc 1739.diseases in aquatic organisms, including fish, are a major concern in aquaculture production. in this present investigation, we have evaluated the beneficial effects of dietary bacillus amyloliquefaciens ccf7 (genbank acc. no. kp256501) supplementation in rohu (labeo rohita) challenged by a pathogenic strain of aeromonas hydrophila mtcc 1739. four experimental diets were formulated: control diet (no probiotics) and three experimental diets (different concentrations of probiotic candidate b. amyl ...201728744833
natural variations in the biofilm-associated protein bsla from the genus bacillus.bsla is a protein secreted by bacillus subtilis which forms a hydrophobic film that coats the biofilm surface and renders it water-repellent. we have characterised three orthologues of bsla from bacillus amyloliquefaciens, bacillus licheniformis and bacillus pumilus as well as a paralogue from b. subtilis called ywea. we find that the three orthologous proteins can substitute for bsla in b. subtilis and confer a degree of protection, whereas ywea cannot. the degree to which the proteins function ...201728751732
small rna profiling reveals important roles for mirnas in arabidopsis response to bacillus velezensis fzb42.bacillus velezensis fzb42 (previously classified as bacillus amyloliquefaciens fzb42) has been confirmed to successfully colonize plant roots and enhance defense response against pathogen infection. this study indicated that fzb42 inoculation enhanced arabidopsis defense response against pseudomonas syringae dc3000 through inducing the expression of pr1, pdf1.2 and stomata closure. to further clarify the induced defense response at mirna level, srna libraries from arabidopsis roots inoculated wi ...201728754632
removal of nitrite from aqueous solution by bacillus amyloliquefaciens biofilm adsorption.a newly verified adsorbent biofilm produced by bacillus amyloliquefaciens dt was investigated for nitrite removal from aqueous solutions. the biofilm's characteristics and adsorption mechanism were determined, with results indicating that nitrite ions were adsorbed onto the protonated amine sites of biofilm under acidic conditions. analysis of various factors showed that higher nitrite adsorption capacities occurred at ph < 3.0 and higher temperatures as well as higher initial nitrite concentrat ...201728756127
isolation and characterization of a bacillus amyloliquefaciens strain with zearalenone removal ability and its probiotic potential.zearalenone (zen) is a non-steroidal estrogenic mycotoxin produced by fusarium species, which has been shown to be associated with reproductive disorders in livestock, and to a lesser extent with hyperoestrogenic syndromes in humans. the aim of this study was to characterize a bacillus amyloliquefaciens strain with zen removal ability. a pure culture of a strain designated ln isolated from moldy corn samples showed a high zen removal capability. based on microscopic observations, biochemical cha ...201728763483
microbial volatile compounds alter the soil microbial community.volatile organic compounds (vocs) from soil bacteria are likely to have an important role in the interactions among soil microorganisms. however, their effects on the soil microbial community have not been extensively studied. in this study, the effect of bacterial vocs generated by growing bacillus amyloliquefaciens njn-6 on modified ms medium on soil microbial community was evaluated. b. amyloliquefaciens njn-6 was able to produce 48 volatile compounds as determined by solid-phase microextract ...201728803260
comparative genomics of bacillus amyloliquefaciens strains reveals a core genome with traits for habitat adaptation and a secondary metabolites rich accessory genome.the gram positive, non-pathogenic endospore-forming soil inhabiting prokaryote bacillus amyloliquefaciens is a plant growth-promoting rhizobacterium. bacillus amyloliquefaciens processes wide biocontrol abilities and numerous strains have been reported to suppress diverse bacterial, fungal and fungal-like pathogens. knowledge about strain level biocontrol abilities is warranted to translate this knowledge into developing more efficient biocontrol agents and bio-fertilizers. ever-expanding genome ...201728824571
optimization of the purine operon and energy generation in bacillus amyloliquefaciens for guanosine production.to deregulate the purine operon of the purine biosynthetic pathway and optimize energy generation of the respiratory chain to improve the yield of guanosine in bacillus amyloliquefaciens xh7.201728840402
previously undescribed antibacterial polyketides from heterotrophic bacillus amyloliquefaciens associated with seaweed padina gymnospora.a heterotrophic marine bacterium bacillus amyloliquefaciens isolated from seaweed padina gymnospora exhibited broad spectra of antibacterial activities against pathogenic bacteria aeromonas hydrophila, vibrio harveyi, vibrio vulnificus, and vibrio parahaemolyticus. the seaweed-associated b. amyloliquefaciens was recognized to possess functional type i polyketide synthase-1 (pks-1) gene, and was used to isolate four homologous compounds with polyketide frameworks. the compounds were characterized ...201728842846
characterization of two novel bacteriocin-like substances produced by bacillus amyloliquefaciens eli149 with broad spectrum antimicrobial activity.the aim of this study was the isolation and characterizationof antifungal and bactericidal compounds from b. amyloliquefaciens strain eli149.201728844975
environmental fate and behaviour of the biocontrol agent bacillus amyloliquefaciens cpa-8 after preharvest application to stone fruit.bacillus amyloliquefaciens strain cpa-8 has been described as an effective biocontrol agent to control brown rot in stone fruit for both preharvest and postharvest applications. however, no information about the environmental fate and behaviour of this strain under field conditions is available.201728846195
antibiosis and bmyb gene presence as prevalent traits for the selection of efficient bacillus biocontrol agents against crown gall disease.this study aimed to improve the screening method for the selection of bacillus biocontrol agents against crown gall disease. the relationship between the strain biocontrol ability and their in vitro studied traits was investigated to identify the most important factors to be considered for the selection of effective biocontrol agents. in fact, previous selection procedure relying only on in vitro antibacterial activity was shown to be not suitable in some cases. a direct plant-protection strateg ...201728855909
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