Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| isolated coxiella burnetii synthesizes dna during acid activation in the absence of host cells. | two populations of coxiella burnetii were isolated from fibroblast tissue cultures and examined for their ability to synthesize dna when incubated in a defined medium. both the populations released by mechanical lysis of heavily infected host cells, as well as those recovered from the tissue culture medium, incorporated h3 32po4 into dna. incorporation occurred at ph 4.5 but not at ph 7.0, and proceeded for 12-15 h. when incorporation of [3h]thymidine was studied, only the organisms obtained by ... | 1990 | 2351956 |
| quantitative evaluation of the effects of human carcinogens and related chemicals on human foreskin fibroblasts. | ten compounds representative of diverse classes of chemicals were evaluated for their cytotoxicity and transforming ability to human skin fibroblasts in vitro. only five of the ten compounds were highly cytotoxic in the 0-100 micrograms/ml range and their order of cytotoxicity was: 2,5-bis(1-aziridinyl)-3,6-bis(carboethoxyamino)-1,4-benzoquinone (azq) greater than cis-platin greater than bis(chloromethyl)ether (bcme) greater than acrylonitrile greater than aflatoxin b1 (afb1). the other five com ... | 1990 | 2361190 |
| [a formalized patient history--a new form of medical documentation]. | in a work, the characteristic of a new form of medical documentation--formalized case record (fcr), which permits to obtain the detailed information about the state of a patient at all the stages of treatment, is given. because of the more and more wide introduction of a computer into the clinical practice, fcr can be considered as a necessary form of medical documentation. the universal character of a fcr permits to use it at different in-patient departments of surgical profile. | 1976 | 2366423 |
| [the interaction of metal-containing carbon sorbents with pathogenic microflora]. | to give sorbents the bactericidal action, a modification of the carbonic sorbents by the metals is suggested. modification of the skn and kau sorbents by copper and zinc didn't reduce sorption by them of microorganisms and their toxins. inhibitory effect of a sorbent was provided by the lower concentrations of the copper and zinc than by those which in the solution of metallic salts had only bacteriostatic effect. | 1976 | 2366427 |
| "our" logo? | 1977 | 2366683 | |
| a comparative study for fiberoptic and video endoscopic determination of the extent in minimal changes of gastric mucosa using indigo dye spraying. | the image resolution of fiberoptic and video endoscopy was compared in a series of patients with early gastric cancer. eighteen patients were divided into two groups. one group of patients (n = 9) was evaluated with fiberoptic endoscopy while the second group (n = 9) was evaluated using video endoscopy. the extent of cancer invasion, with special attention to size, shape and type of the lesions, was evaluated in each patient. attempts were also made to identify the proximal and distal limits of ... | 2007 | 2374986 |
| localization of dna in coxiella burnetii by post-embedding immunoelectron microscopy. | the deoxyribonucleic acid of coxiella burnetii was detected with monoclonal antibodies against single-stranded and double-stranded dna by post-embedding immunoelectron microscopy. the antibody labeled the nucleoid of the spore, the small cell variant (scv), and the large cell variant (lcv). the dna was segregated completely in both the spore and the mother cell. at the terminal stage of development of the spore, the loss of nuclear morphology in the mother cell was related to the reduction in th ... | 1990 | 2378445 |
| the immune response to phase i and phase ii coxiella burnetii antigens as measured by western immunoblotting. | to determine the utility of western immunoblotting in distinguishing chronic q fever from acute q fever, we first examined serum samples from individuals who had no antibodies to coxiella burnetii by either the indirect immunofluorescence antibody test or by the enzyme-linked immunosorbent assay. in these subjects, the igg fraction in low dilutions of serum (1:8, 1:16) reacted with as many as 10 proteins in phase i and phase ii antigens. this number of reacting bands seen in western blots was re ... | 1990 | 2378447 |
| rickettsia in texas. | since the first reported case in 1941, rocky mountain spotted fever in humans has been reported from many areas of texas, with two major foci, one located in the north-central region and the other in the eastern region of the state. during the period 1979-1988, 421 cases of rmsf were reported, reaching 108 cases in 1983 and declining in subsequent years. statewide surveillance programs to detect spotted fever group rickettsiae in tick populations were initiated in 1976. in recent years, the sfg ... | 1990 | 2378448 |
| q fever in octogenarians. | two of the 186 persons (1%) with acute q fever examined in this study were 80 years of age or older. both were males and both had coxiella burnetti pneumonia. q fever was not suspected during their hospital stay and would not have been diagnosed if these patients had not been part of a prospective study of community-acquired pneumonia. | 1990 | 2378454 |
| the role of lipopolysaccharides in the virulence of coxiella burnetii. | 1990 | 2378455 | |
| seroepidemiology of q fever among cats in new brunswick and prince edward island. | the indirect microimmunofluorescence test was used to determine the presence of antibodies to phase i and phase ii coxiella burnetii antigens in new brunswick and prince edward island cats. twenty of 104 (19.2%) new brunswick cats tested had antibodies to phase ii antigen; five of these (4.8%) also had antibodies to phase i antigen. six of 97 (6.2%) prince edward island cats tested had antibodies to phase i and phase ii antigens. our data suggest that cats may be important in the epidemiology of ... | 1990 | 2378456 |
| incidence of q fever: pilot studies in two areas in nova scotia. | we carried out two pilot studies to determine the incidence of q fever in nova scotia. the first study involved 199 people from the annapolis valley region. the first set of blood samples was collected between may and september and the second three to five months later. two subjects had titers greater than or equal to 1:256 (by the indirect immunofluorescence test), suggesting recent infection. the second group studied consisted of 95 residents of baddeck. nova scotia. their first serum samples ... | 1990 | 2378457 |
| q fever in sokoto, nigeria. | there is very little information available about the prevalence of coxiella burnetii in africa. we obtained blood from 75 nigerians hospitalized in sokoto for a variety of acute medical conditions. their age range was 7 months to 50 years; there were 39 males and 36 females. antibody titers were determined to phase i and phase ii c. burnetii antigens using a microimmunofluorescence test. thirty-three (44%) had an antibody titer of greater than or equal to 1:8 to phase ii c. burnetii. we conclude ... | 1990 | 2378458 |
| occupational health guidelines for control of q fever in sheep research. | 1990 | 2378459 | |
| susceptibility of coxiella burnetii to chemical disinfectants. | 1990 | 2378460 | |
| unexpected antibiotic susceptibility of a chronic isolate of coxiella burnetii. | evidence is mounting in support of the idea that different isolates of coxiella burnetii, the etiologic agent of q fever, are responsible for the distinct disease syndromes observed clinically. recent studies have shown distinct antibiotic susceptibilities of different isolates of c. burnetii implicated in distinct clinical q fever syndromes. with this in mind, we performed antibiotic susceptibility testing of the "s" isolate, a chronic-type isolate retrieved from a human patient with chronic di ... | 1990 | 2378461 |
| antigenic structure of coxiella burnetii. a comparison of lipopolysaccharide and protein antigens as vaccines against q fever. | the antigenic structure of coxiella burnetii is being investigated by identifying both external and internal cellular epitopes of the morphologic cell types. both the phase i lipopolysaccharide (lpsi) and several surface proteins are candidates for the development of subunit multivalent vaccines. the protective efficacy of purified lpsi was demonstrated in a/j mice. the purified lpsi preparations contained residual peptides detected by amino acid analysis. therefore, the protection afforded by l ... | 1990 | 2378463 |
| the biology of coxiella burnetti and the pathobiochemistry of q fever and its endotoxicosis. | c. burnetii possesses a battery of host-independent enzymes which mediate endergonic and exergonic reactions. the biochemical and biophysical lesions responsible for the organism's obligate intracellular parasitic state have not been identified. clues to this fundamental problem may lie in the agent's acidophilic metabolism and proliferation within the host cell's phagolysomal vacuole. what are the modifiers of transcription and translation? what constituents of the lysosomal vacuole contribute ... | 1990 | 2378468 |
| dna probes for the identification of coxiella burnetti strains. | isolation of coxiella burnetii in the standard laboratory setting is hazardous; therefore most diagnoses are based on retrospective detection of a rising antibody titer to c. burnetti. as a result, this disease is usually undiagnosed or misdiagnosed. methods for the rapid detection of c. burnetti have now been developed that utilize specific hybridization of labeled dna probes to nucleic acid in clinical samples. one method detects the presence of c. burnetii 16s ribosomal rna (rrna); another us ... | 1990 | 2378470 |
| characterization of coxiella burnetii pyrb. | the c. burnetii pyrb gene was cloned on a 7-kbp ecor i fragment. dna sequence analysis, enzyme assays, and amino acid homologies with e. coli and b. subtilis pyrb gene products suggest that (i) c. burnetii atcase exists as a trimer, (ii) the microorganism may not synthesize a regulatory polypeptide, and (iii) pyrb may be part of an operon whose expression is under the control of an upstream promoter. the high degree of homology of the active site further suggests that a common mechanism of catal ... | 1990 | 2378471 |
| use of pulsed field gel electrophoresis to differentiate coxiella burnetii strains. | pulsed field gradient gel electrophoresis (pfge) provides a powerful technique for the analysis of bacterial genomic dna by allowing the resolution of dna fragments as large as 9000 kilobase pairs (kbp). when isolates of coxiella burnetii were examined using this method, the restriction enzymes not i and sfi i gave the fewest and most easily resolved fragments. sfi i cuts the genome of the priscilla isolate of c. burnetii into 15 dna fragments ranging in size from 320 to 18 kbp, and not i cuts t ... | 1990 | 2378472 |
| chronic q fever: diagnosis and follow-up. | sera from 40 patients (25 men, and 15 women) with clinical features compatible with the diagnosis of chronic q fever were received. total or partial clinical data were available. all of them had serological evidence of chronic q fever (igg class anti-phase i titer greater than 800). the final diagnosis was vascular infection in four cases (with two positive cultures for coxiella burnetii), bone infection in two patients (one positive culture), chronic hepatitis in one patient, and endocarditis i ... | 1990 | 2378473 |
| analysis of qprs-specific sequences from coxiella burnetii. | coxiella burnetii from acute cases of q fever possess a plasmid termed qph1. chronic isolates contain a plasmid termed qprs or have qprs sequences integrated into the chromosome. the correlation between an isolate's plasmid type and the chronic or acute nature of the disease has prompted analysis of unique plasmid sequences to determine if they contain virulence genes. dna hybridization has determined that a portion of a 3.6-kb ecor i fragment (epsilon') is unique to qprs. in vitro transcription ... | 1990 | 2378474 |
| comparison of coxiella burnetii plasmids to homologous chromosomal sequences present in a plasmidless endocarditis-causing isolate. | coxiella burnetii is the etiological agent of human q fever and chronic endocarditis. different plasmids have been found in c. burnetii isolates and a correlation between disease state and plasmid type has been established. the plasmid qprs was found in all but four of the endocarditis-causing isolates examined. these four isolates did not contain a detectable plasmid. however, when dna from the plasmidless isolates is hybridized with 32p-labeled qprs, homologous sequences are detected. it was h ... | 1990 | 2378475 |
| identification and cloning of a 27-kda coxiella burnetii immunoreactive protein. | patients with acute q fever develop coxiella burnetii-specific antibody, and chronic disease patients often develop extremely high levels of c. burnetii-specific antibody. antibody-reactive lps has been well characterized, but only a few immunoreactive proteins have been identified. an immunoreactive ca. 62-kda protein has been cloned, sequenced, and shown to be related to an escherichia coli heat-shock protein. a ca. 27-kda immunoreactive outer membrane protein(s) has also been identified. we h ... | 1990 | 2378476 |
| strategy for detection and differentiation of coxiella burnetii strains using the polymerase chain reaction. | a method for the rapid detection of coxiella burnetii and differentiation between strains that cause endocarditis and those that cause acute q fever is based on the observation that the different strains contain unique plasmid sequences. this method employs the polymerase chain reaction (pcr) and requires knowledge of specific dna sequences in the region (target) of dna to be amplified. to detect and differentiate between c. burnetii isolates, two sets of primers are required. the first set was ... | 1990 | 2378478 |
| a comparison of q fever endocarditis with native valve endocarditis. | we compared 10 episodes (8 patients) of q fever endocarditis with 27 episodes (27 patients) of native valve endocarditis. patients with q fever endocarditis were more likely to have weight loss (p less than 0.003), experience fatigue (p less than 0.07), have clubbing of the fingers (p less than 0.005), have a diastolic murmur at the time of admission (p less than 0.03), be anemic (p less than 0.05), have a normal white blood cell count (p less than 0.005), and have a higher serum globulin concen ... | 1990 | 2378480 |
| ciprofloxacin in a case of q fever endocarditis. | 1990 | 2381451 | |
| endotoxin-induced uveitis in the rat: a hypothesis for preferential involvement of the anterior uvea. | endotoxin-induced uveitis (eiu) was produced in lewis rats by footpad injection of salmonella endotoxin. protein and cells were measured both in the aqueous humor and in the cerebrospinal fluid (csf) in order to examine if the inflammation was strictly limited to the anterior uvea. eiu was also induced in fischer and brown norway rats and the inflammation was compared among the three strains. although the function and structure of the choroid plexus is very similar to the ciliary body, no signs ... | 1990 | 2384004 |
| characterization and expression of the cbbe' gene of coxiella burnetii. | a gene which is unique to the qprs plasmid from chronic isolates of coxiella burnetii was cloned, sequenced, and expressed in escherichia coli. this gene, termed cbbe', codes for a putative surface protein of approximately 55 kda, termed the e' protein. the cbbe' gene is 1485 bp in length, and is preceded by predicted promoter regulatory sequences of tttaat (-35), tataat (-10), and a shine-dalgarno sequence of ggagaga, all of which closely resemble those of e. coli and other rickettsiae. the ope ... | 1990 | 2384744 |
| effect of neuromuscular blockade on fetal oxidative metabolism. | because fetal neuromuscular blockade has been used during cord blood sampling procedures to prevent fetal movement, we studied the effect of pancuronium bromide on fetal arterial concentrations of glucose, lactate, and oxygen and their umbilical uptake in 6 chronically prepared fetal sheep. we observed a significant increase in the arterial concentrations and decrease in the umbilical uptakes. we suggest that cord blood sampling procedures for fetal assessment of oxidative metabolism not be perf ... | 1990 | 2390540 |
| the cyclotron saga. | 2008 | 2390557 | |
| miller fisher syndrome associated with q fever. | a 51 year old woman with pneumonia developed miller fisher syndrome. indirect immunofluorescence tests showed antibodies against coxiella burnetti. miller fisher syndrome associated with q fever has not been described previously. | 1990 | 2391529 |
| role of ceramide as a lipid mediator of 1 alpha,25-dihydroxyvitamin d3-induced hl-60 cell differentiation. | the treatment of hl-60 myelocytic leukemia cells with 1 alpha,25-dihydroxyvitamin d3 (1,25-(oh)2d3) resulted in the activation of a neutral sphingomyelinase and in sphingomyelin turnover (okazaki, t., bell, r., and hannun, y. (1989) j. biol. chem. 264, 19076-19080). in this paper, the effects of 1,25-(oh)2d3 on the product of sphingomyelin hydrolysis, ceramide, and the possible function of ceramide as a lipid mediator of the effects of 1,25-(oh)2d3 on hl-60 cell differentiation were investigated ... | 2007 | 2394750 |
| a simple method for protein electrophoresis of unconcentrated urine. | thirty-two unconcentrated urines were electrophoresed on cellogels in 0.05 m barbital buffer, ph 8.6, at 280 volts for 35 minutes. instead of concentrating 100 fold, the urine samples were directly applied on the gel in volumes from 1.2 to 3.6 microliters and coomassie dye was used for staining. this makes it possible to observe the protein patterns at trace concentration by reagent strips (about 0.05-0.2 g/l). this method is simple, convenient and suited for routine services. | 2008 | 2394956 |
| critical period for the androgenic block of neuromuscular synapse elimination. | juvenile androgen treatment during developmental synapse elimination changes the pattern of innervation in the adult levator ani (la), an androgen-sensitive muscle (jordan, letinsky, and arnold, 1989b). most notably, such adult muscles contain an unusually high number of muscle fibers that are innervated by two or more axons indicating that these fibers are multiply innervated. juvenile androgen treatment also increases the adult level of preterminal branching, the number of junctional sites per ... | 1990 | 2394990 |
| comparison of nonsteroidal anti-inflammatory drugs, ibuprofen and flurbiprofen, with methylprednisolone and placebo for acute pain, swelling, and trismus. | pain, swelling, loss of function, and hyperthermia are acute postoperative sequelae of inflammation due to tissue injury during surgical procedures. pharmacologic strategies for minimizing the clinical manifestations of surgical trauma are often directed toward blocking the formation or inhibiting the effects of the biochemical mediators of acute inflammation. this study compared two nonsteroidal anti-inflammatory drugs (nsaids), flurbiprofen and ibuprofen, with a prototype glucocorticoid, methy ... | 2008 | 2395047 |
| variation in macronutrients in human bank milk: influencing factors and implications for human milk banking. | protein (p), fat (f), and carbohydrate (c) concentration in expressed human bank milk was determined by infrared analysis of 2,554 samples from 224 mothers. the mean contents of p, f, c, and energy (e, calculated from p, f, and c) were 9.0 g/l, 39.0 g/l, 71.9 g/l, and 696 kcal/l, respectively. there was a large variation in the concentration of energy-yielding macronutrients. the contents of p, f, c, and e in the samples with the highest values (97.5 percentile) were 2.3-, 4.8-, 1.2-, and 2.3-fo ... | 1990 | 2395063 |
| use of the megasoft bandage lens for treatment of complications after trabeculectomy. | shallow anterior chambers and leaking filtration blebs are possible complications after trabeculectomy that can be treated with therapeutic contact lenses. in most cases, however, treatment fails because these lenses are not large enough to cover the filtering bleb. we evaluated the use of a newly developed large diameter (20.5 mm) therapeutic soft contact lens. five patients with shallow anterior chambers and ten patients with leaking filtering blebs after trabeculectomy were fitted with this n ... | 2008 | 2396651 |
| rat organum vasculosum laminae terminalis in vitro: responses to changes in sodium concentration. | extracellular action potentials were recorded from the organum vasculosum laminae terminalis (ovlt) in rat brain slice preparations; the effect of different concentrations of nacl on spontaneous firing frequency was studied. from 72 neurons, 67 (93%) were responsive to various perfusion media, while 5 neurons (7%) were not responsive. the change from the standard medium (sm; 124 mm nacl) to 99 mm, decreased the firing frequency in 24 (65%) and increased it in 13 (35%) out of 37 responsive cells. ... | 1990 | 2397412 |
| [the clinical spectrum of rickettsiosis in the area of zadar]. | the clinical spectrum of rickettsioses in patients admitted to our hospital (department of infectious diseases, general hospital of zadar) from 1983 to 1988 has been analysed. this spectrum consisted of the following diseases: murine typhus, q-fever, boutonneuse fever and brill-zinsser disease. the low number of hospitalized patients with rickettsioses was in disproportion with the prevalence of antibodies to these rickettsiae in general population of the zadar area. our results indicate that th ... | 2009 | 2398776 |
| cholesterol testing. | 2008 | 2398789 | |
| cellular immunity in q fever: modulation of responsiveness by a suppressor t cell-monocyte circuit. | human infection with the rickettsia coxiella burnetii presents as an acute flulike primary q fever, as a subacute granulomatous hepatitis, or, rarely, as chronic endocarditis. we have previously described lymphocyte unresponsiveness to coxiella antigen in patients with q fever endocarditis. this unresponsiveness was antigen specific and was mediated in part by adherent suppressor cells. in this report we show that the adherent suppressor cells work via prostaglandin e2 (pge2)4 production. additi ... | 1985 | 2409135 |
| antibody responses in acute and chronic q fever and in subjects vaccinated against q fever. | an analysis is made of the antibody response to coxiella burnettii phase-1 and phase-2 antigens, as measured by immunofluorescence in the igm, igg or iga immunoglobulin classes, or by complement-fixation, in patients with acute and chronic q fever and in vaccinated or skin-tested subjects. in acute (primary) q fever, igm specific antibodies to phase-1 antigen are present in early convalescence together with igm, igg, iga and cf antibodies to phase-2 antigen. igm specific antibody may persist for ... | 1985 | 2409285 |
| monoclonal antibodies reactive with epitopes restricted to glial fibrillary acidic proteins of several species. | the highly reproducible histochemical localization of glial fibrillary acidic protein (gfap)+ qualifies it as an important marker of astrocytes in both research and clinical applications. the primary objective of this study was to produce monoclonal antibodies having the advantage of invariant specificity, affinity, and titer to gfap-specific epitopes of wide species distribution. we report here the characterization of four monoclonal antibodies that recognize the same or spatially close epitope ... | 1985 | 2413405 |
| cellular immunity in q fever: specific lymphocyte unresponsiveness in q fever endocarditis. | human infection with the rickettsia coxiella burnetii presents as acute influenza-like primary q fever, subacute granulomatous hepatitis, or chronic endocarditis with hepatitis. to investigate whether persistent infection is associated with a possible immunologic defect, we tested lymphocyte proliferation specific for coxiella in vitro in peripheral blood mononuclear cells from patients and controls. all four patients with endocarditis had profound lymphocyte unresponsiveness to coxiella antigen ... | 1985 | 2415642 |
| [effects of enzymatic deglycosylation of human goiter thyroglobulin on its immunochemical properties]. | thyroglobulin (tg), isolated from soluble iodoproteins by ammonium sulphate fractionation, was enzymatically deglycosylated in vitro and analyzed by polyacrylamide gel electrophoresis, double immunodiffusion and non-commercial ria. carbohydrate and iodine content was chemically determined. by paage deglycosylated tg (dtg) showed the appearance of a major band in the 12s region and three slower migrating bands corresponding to higher aggregates than 19s tg. in immunodiffusion by testing native an ... | 1986 | 2430684 |
| correlation of dna, rna, and protein content by flow cytometry in normal and coxiella burnetii-infected l929 cells. | l929 cell populations persistently infected for more than 600 days exhibited cell cycle distributions and generation times similar to those of uninfected cells. the rna and protein contents of these long-term-infected cells, as determined by flow cytometry and correlated with the cell cycle, were likewise approximately the same as those in normal uninfected cells. also, the ratios of rna to dna and rna to protein of infected cells were not significantly different from those of normal cells. coll ... | 1987 | 2439460 |
| antiviral treatment of chronic type b hepatitis. | 1987 | 2441635 | |
| changes in cellular gene expression in rat thyroid epithelial cells transformed by the kirsten murine sarcoma virus. | we have exploited a recently characterized system of rat thyroid epithelial cells transformed by the wild-type (wt) and a temperature-sensitive (ts) mutant strain of the kirsten murine sarcoma virus (ki-msv) in order to study the effects of the k-ras oncogene on the gene expression of differentiated thyroid epithelial cells. by using cdnas isolated from normal thyroid glands as probes, we were able to identify three sets of cellular sequences whose expression is influenced by the v-k-ras oncogen ... | 1987 | 2442021 |
| recurrent inhibition of firing motoneurones in man. | the effects of antidromic stimulation of large motoneurones on firing small motoneurones of soleus muscle have been studied. against the background of rhythmic firing of small motor units (mus) activated during weak voluntary muscle contraction, thick efferents of the tibialis posterior nerve were selectively stimulated and an m response was evoked in which small mus were not involved. this provided a means of avoiding antidromic stimulation of the motoneurone under study and, thus, analysing th ... | 1988 | 2446837 |
| binding characteristics and proliferative action of purified granulocyte colony-stimulating factor (g-csf) on normal and leukemic human promyelocytes. | the binding of purified 125i-labeled murine granulocyte colony stimulating factor (125i-g-csf) to normal and leukemic human cells was examined. normal neutrophils and their precursors demonstrated specific labeling with 125i-g-csf, whereas eosinophils, lymphocytes, and erythroid cells did not. normal human promyelocytes demonstrated the highest binding among hemopoietic cells. human myeloid leukemic cells also demonstrated consistent specific labeling with 125i-g-csf. normal promyelocytes and ch ... | 1988 | 2446901 |
| substrate binding domain of murine leukemia virus reverse transcriptase. identification of lysine 103 and lysine 421 as binding site residues. | the substrate deoxynucleoside triphosphate (dntp) binding site of moloney murine leukemia virus (m-mulv) reverse transcriptase was labeled with pyridoxal 5'-phosphate (plp), a substrate binding site-directed reagent for dna polymerases (modak, m. j. (1976) biochemistry 15, 3620-3626). treatment of m-mulv reverse transcriptase with plp results in the loss of rna-dependent dna polymerase activity, but has no effect on ribonuclease h activity. neither template-primer nor substrate dntp alone shows ... | 1988 | 2448299 |
| stability of plasmid sequences in an acute q-fever strain of coxiella burnetii. | the rickettsial pathogen coxiella burnetii undergoes a variation in which virulent isolates (phase 1) become avirulent (phase 2) after repeated passage in a non-immunologically competent host. biochemically, this variation is associated with a lipopolysaccharide modification and possibly other factors. genetically, the regions of dna responsible for phase variation have not been identified. we have sought to determine whether the plasmid identified in acute disease isolates, qph1, which represen ... | 1988 | 2469774 |
| cytokine-induced respiratory burst of human neutrophils: dependence on extracellular matrix proteins and cd11/cd18 integrins. | human polymorphonuclear leukocytes (pmn) released large quantities of hydrogen peroxide in response to tumor necrosis factor, but only when the cells were adherent to surfaces coated with extracellular matrix proteins. the pmn did not respond when exposed to cytokines and matrix proteins in suspension, or when exposed to cytokines while adherent to surfaces coated with stearic acid. pmn from children with genetic deficiency of the cd11/cd18 integrins underwent a normal respiratory burst upon adh ... | 1989 | 2475511 |
| [prevalence of antibodies against coxiella burnetii in 2 geographical zones of tuscany]. | q fever, caused by coxiella burneti, was first identified as a separate syndrome in 1937 in australia. usually it takes the form of an acute atypic lung pneumonia disease, but subclinic or non-typical forms are also known. q fever is a zoonoses. the most important source of infection is represented by sheep; the transmission to man occurs by vectors (ticks), or more often by carriers as aerosol, non-pasteurized milk and dairy products. in italy the first outbreaks of q fever date back to the end ... | 1989 | 2483896 |
| [properties of phase i purified cellular antigen of coxiella burnetii]. | an attempt was made to purify phase i cell suspension of coxiella burnetii used as an antigen in diagnostic serological tests. homogenised suspension of chick embryos infected with phase i henzerling and "z" strains, after preliminary purification from host cell contaminants of chick embryos was subjected to consecutive centrifugation in sucrose/uropoline gradient and to continuous 20-45% uropoline gradient. the fractions obtained from uropoline gradient centrifugation were applied as phase i an ... | 1989 | 2484257 |
| [methods of isolating phospholipids from coxiella burnetii]. | phospholipids are a part of the outside membrane of coxiella burnetii (or other gram negative bacteria). it is possible to extract them with a mixture of chloroform-methanol (cm). for extraction of phospholipids from c. burnetii it is important to take account of temperature, proportion of cm, and reaction time. the best results are obtained at cm 2:1, at boiling point of this mixture and reaction time of 2 hours. longer reaction time does not influence the reaction, the additional amounts of ph ... | 1989 | 2485294 |
| [immunogenic and immunomodulating properties of phospholipids from coxiella burnetii]. | it is proved that the challenging of the extract isolated from coxiella (as a result of treatment with the mixture of chloroform and methanol) to animals doesn't provoke the appearance of antibodies to these microorganisms. it also doesn't cause the sensibilization of lymphocytes to coxiella, but the extract can interact specifically with a serum containing antibodies to coxiella. it permits to characterise the tested extract of coxiella as a hapten with properties of adjuvant because it stimula ... | 1989 | 2485295 |
| [solid-phase immunoenzyme analysis (elisa)--a test for diagnosing q fever (comparison with the complement fixation and immunofluorescence methods)]. | purified phase i coxiella burnetii corpuscles treated chemically in different ways (by potassium periodate, mild acid hydrolysis or trichloroacetic acid) were compared by elisa for their ability to detect phase ii (directed to antigen 2) and phase i (directed to antigen 1) antibodies in sera from human q fever convalescents. as to the absorbance values, the most sensitive was the antigen obtained by mild acid hydrolysis (0.1 mol/l hcl for 30 min. at 100 degrees c), followed by phase i corpuscula ... | 1989 | 2485296 |
| [detection of antibodies to coxiella burnetii antigens using immunoenzyme analysis]. | in trials the optimal conditions of immuno-enzyme analysis for detection of antibodies to coxiella burnetii were revealed. it was proved that the sensitivity of elisa exceeds the sensitivity of reaction of complement binding not less than in 100 times. the effectiveness of elisa was shown to be higher than that of reaction of complement binding both at early terms of disease (up to 14th day) and in persons having the disease rather long ago (1-2 years ago). | 1989 | 2485297 |
| [an indirect solid-phase immunoenzyme method in diagnosing rickettsial infections]. | sera of brill-zinsser disease patients (70 samples) q fever patients (12 samples) and healthy persons from q fever enzootic areas (99 samples) were studied in indirect solid phase enzyme immunoassay (elisa), indirect immunofluorescent antibody test (ifa) and indirect passive hemahglutination test. elisa and ifa have been proved as most sensitive tests for detection of rickettsial antibodies in both clinical and retrospective diagnosis of rickettsial diseases. | 1989 | 2485298 |
| [experience in vaccinating farm animals for preventing q fever in humans]. | vaccination of animals is indicated for cattle and sheep to prevent human infections deriving from these animals and inhibit the infertility in livestock. both phase i and phase ii are available. vaccination is successful only when animals had been vaccinated as noninfected young, i. e. calves or lambs. corpuscular vaccines are prevalent in immunoprophylaxis of domestic animals against coxiellosis. such vaccines consist of formalin--inactivated, purified corpuscules coxiella burnetii in phase i ... | 1989 | 2485300 |
| [the effect of serial passage of coxiella burnetii in chick embryos on pathogen phase variability]. | coxiella burnetii has been investigated in different egg passages (ep-17, 163). coxiella in ep 163 was completely eliminated from the suspension during the purification but not that in ep 17. it means that the coxiella of the two ep with a seroreaction of phase ii are different, and phase ii positivity of ep 17 can't be the result of presence of two populations in the suspension or of one mutation during the multiplication in yolk sacs but of a change on the surface of c. burnetii. | 1989 | 2485301 |
| [results of epidemiological surveillance for q fever in the voronezh area]. | the represented materials characterise q-fever situation in voronezs region basing on data of long term observations. three outbreaks and regularity of epidemiological process of this infection are described. | 1989 | 2485304 |
| [epidemiological characteristics of q fever foci in the novosibirsk area]. | analysis of q-fever morbidity and serological studies of representative extracts of population, agricultural and wild animals permitted to reveal the importance of inner-herd foci in epidemiology of this infection. it is shown that the level of registered morbidity mainly depends on the state of q-fever laboratory diagnostics. | 1989 | 2485305 |
| [the epidemiology of q fever]. | q fever as one of infections with natural foci is characterized by epidemic outbreaks. biologic plasticity and ecologic peculiarities of coxiella determine its wide distribution and development of q fever infections which are disseminated almost every-where. they occur in two, i. e. natural and agricultural types of foci. present paper characterizes their structures, maintenance and peculiarities if their existence. it should be emphasized that single cases of disease as well as outbreaks of q f ... | 1989 | 2485306 |
| [perfection of epidemic surveillance for q fever based on optimizing its diagnosis]. | for the first time q-fever was diagnosed in the east region of the ussr. criteria system on selection of patients with vague diagnosis for laboratory examination on q-fever was worked out and "risk" groups were determined by analysing 204 cases of cocciellosis and studying of immunological structure. | 1989 | 2485307 |
| [relapse of q fever in childbirth and transmission of the pathogen to the child]. | a case was described when a newborn was inoculated with coxiella burnetti as a result of infection relapse (in 8 months after acute disease) in his mother. coxiella burnetii were isolated from blood of both patients. dynamics of humoral immunity indices in mother was observed both at the primary disease and during the period of infection relapse. | 1989 | 2485308 |
| [nest ectoparasites (gamasid mites) as vectors for rickettsia under experimental conditions]. | the author gives a review of occurrence of rickettsiae (coxiella burnetii, r. slovaca, r. sibirica, r. prowazekii, r. mooseri, r. akari) in gamasid mites, investigated in laboratory or in nature. the results of our experiments and from literature also are summarized in tables. the experimental infection with c. burnetii was investigated by natural route of infection in the following species of mites: haemogamasus nidi, h. hirsutus, androlaelaps casalis. we found c. burnetii in smears, stained wi ... | 1989 | 2485310 |
| [interaction of live and killed coxiella burnetii with model animal cells at the ultrastructural level]. | materials are submitted on electron-microscopic studying of alive and inactivated coxiella in their interaction with murine cells during 28 days observations. the most variability of coxiella morphologic types is shown to be found in spleen. the structure of endospora type is described. several types of phagosomes are revealed and described. | 1989 | 2485311 |
| [myositis and rhabdomyolysis caused by q fever]. | 1989 | 2490870 | |
| [acute hepatitis as a presenting form of q fever]. | 1989 | 2490871 | |
| [q fever on the island of la palma. a review of 35 patients]. | we present 35 patients diagnosed as suffering from q fever during 1986, 1987, 1988. we establish a diagnosis criteria of complement titres greater than or equal to 1/64 or indirect immunofluorescent titres (iit) 4 or more times higher than the initial, or a maintained title greater than or equal to 1/640 with specific igm detected by positive iit. 80% were male with a median age of 40.3 years. 85% had had contact with goats. 77% lived on the west side of the island 88.5% had increased got and gp ... | 1989 | 2491047 |
| [cholostasic jaundice as manifestation of the hepatic form of q fever]. | 1989 | 2491194 | |
| a review of experimental studies of iodine deficiency during fetal development. | iodine deficiency is now recognized as a major international public health problem. it is estimated that 800 million people may be at risk of the effects of iodine deficiency. in humans, the effects occur at all stages of development: the fetus, the neonate, the child and adult. the effects are now denoted by the term iodine deficiency disorders (idd). they include miscarriages, stillbirths, congenital anomalies, as well as the more familiar goiter, cretinism, impaired brain function, and hypoth ... | 1989 | 2493080 |
| hemocyte monophenol oxidase activity in mosquitoes exposed to microfilariae of dirofilaria immitis. | monophenol oxidase (mpo) activity in hemocytes collected from aedes aegypti liverpool strain and aedes trivittatus intrathoracically inoculated with saline alone, inoculated with dirofilaria immitis microfilariae (mff), or from uninoculated mosquitoes was compared using a radiometric tyrosine hydroxylation assay. hemocyte mpo activity in mff-inoculated (= immune-activated) mosquitoes was significantly increased at 24 hr postinoculation (pi) in a. aegypti and at 6, 12, and 24 hr pi in a. trivitta ... | 1989 | 2493084 |
| bacillus cereus panophthalmitis associated with intraocular gas bubble. | it has become increasingly apparent that bacillus cereus can cause a severe and devastating form of endophthalmitis following penetrating trauma by a metallic object. b. cereus is an uncommon aetiological agent in non-clostridial gas-forming infections. the patient studied in this single case report showed evidence of intraocular gas mimicking gas gangrene infection. the physiology of non-clostridial bacteria producing gas from anaerobic metabolic conditions is reviewed. further intraocular and ... | 1989 | 2493262 |
| [q fever infectious endocarditis. apropos of a new case]. | we report a case of coxiella burnetii endocarditis in a 42-year old man presenting with a long-known cardiac murmur and an infectious syndrome of several months duration. the aetiological diagnosis, delayed by the lack of knowledge of a primary q fever, was established by serology. the infection responded to tetracycline combined with cotrimoxazole, but a valve replacement performed for haemodynamic reasons was followed by serious complications. we remind the readers that q fever endocarditis mu ... | 1989 | 2500089 |
| [the most frequent infectious causes of abortion in sheep in north bavaria with special reference to chlamydia and salmonella infections]. | between 1980 and 1987 1153 ovine fetuses and placentas were examined after abortion. in 68.5% of the cases a cause of abortion could be diagnosed as follows: 43.5% chlamydia psittaci var. ovis, 10.7% salmonella abortus ovis, 3.7% coxiella burnetii, 3.3% listeria monocytogenes and 0.4% campylobacter fetus intestinalis. hemolyzing escherichia coli and streptococci, yersinia pseudotuberculosis, salmonella typhimurium, salmonella enteritidis, corynebacterium pyogenes and other facultative pathogens ... | 1989 | 2503908 |
| identification of two unknown reading frames in synechococcus 6301 as homologues of the 10k and 65k antigen genes of mycobacterium tuberculosis and related heat shock genes in e. coli and coxiella burnetii. | 1989 | 2505234 | |
| [veterinary aspects of zoonose-related diseases in humans]. | the paper presents the possibilities, difficulties and inabilities of veterinary medicine in the prophylaxis of zoonotic diseases in humans. with reference to this, the author characterized salmonella paratyphi induced alimentary toxicon-infections, q fever and chlamydiosis. the article also discusses current views on some neoplastic diseases in animals and their possible transmission to humans. | 1989 | 2510217 |
| [late-infantile form galactosialidosis with psychomotor retardation and spastic paraparesis]. | we described a case of late-infantile form of galactosialidosis. this male patient was a product of normal pregnancy. his parents were first cousins. he first sat at eight months, walked and talked at two years of age. his gait gradually became unsteady and he was diagnosed as spastic paraparesis at the age of five years. abnormally slow learning was first pointed out at seven years of age. at the age of nine years, we evaluated him in detail at our university hospital. physical examination reve ... | 2008 | 2514061 |
| [q fever, aortic insufficiency, hypersplenism and monoclonal gammapathy]. | 2016 | 2518701 | |
| [the submental artery of the cat]. | this study was made on the origin, ramifications and distribution features of the submental artery in 50 adult cats utilizing acryl plastic injection method, with discussion about the comparative angiology of the maxillo-facial region. the submental artery of the cat arose from the facial artery at the posterolateral margin of the mylohyoideus muscle in an aspect of a course continuation of the facial artery. but the submental artery in one case arose directly from the external carotid artery at ... | 1989 | 2519291 |
| bites and stings. part 2. bees and vespids. | 2008 | 2519523 | |
| [coxiella burnetti: serologic study on bovine from the community of madrid]. | complement fixative antibodies are determined with regard to coxiella burnetii in phase ii, in 106 serum samples taken from cattle in the madrid region. the overall prevalence of antibodies was 66.98% and 11.32% was high titer. the result obtained are compared with the results from other studies. | 1989 | 2519700 |
| [clinico-epidemiologic characteristics of q fever]. | the authors describe the clinical aspects and epidemiology of q fever in the ukrainian ssr and neighbouring territories of the russian sfsr. using clinico-epidemiological data as corresponding criteria for the diagnosis of infection by means of adequate laboratory diagnostic tests permitted to increase the detection rate of q fever. | 1989 | 2532807 |
| multiple low dose streptozocin-treated rats: biochemical and morphological effects of cyclosporin a administration. | biochemical and morphological modifications occurring in the pancreas of multiple low dose streptozocin treated rats, subjected to a preventive or concomitant immunosuppressive administration of cyclosporin a (cya 8mg/kg b. wt./45 days), were observed. biochemical assays showed that cya significantly enhances glycaemia (p less than 0.0001) and decreases serum insulin levels (p less than 0.0001), while serum creatinine was slightly increased (p less than 0.05). morphological findings (l.m. and t. ... | 1989 | 2532961 |
| [geographical variants in the immunological structure of the population in combined foci of tick-borne encephalitis and endemic rickettsiosis in the european part of the ussr]. | the zonal study of the immunological structure of the population revealed an increase in the number of infective agents with which the population comes into contact in the southern regions. in the medial boreal forest zone contacts of low intensity only with tick-borne encephalitis virus were detected (the immune stratum was found to be 5.5%, 2,178 persons were examined); in the southern boreal forest zone the foci of tick-borne encephalitis actively manifested (the immune stratum reached 38.9%, ... | 1989 | 2534255 |
| [gastrointestinal lymphomas. clinicopathologic update]. | 2008 | 2535564 | |
| different priming on human neutrophil respiratory burst by lipopolysaccharides from phase i and phase ii coxiella burnetii. | human neutrophils produce small amounts of superoxide anion when stimulated with the chemotactic peptide fmlp; preincubating neutrophils with low concentrations of lipopolysaccharides (lps) markedly increases this response, an effect referred to as priming. in this work lps from coxiella burnetii either phase i (virulent) or phase ii (avirulent) were examined for their ability to induce priming. results clearly show that only lps from phase ii microorganism was able to increase the release from ... | 1989 | 2541318 |
| brain leukotriene c4 binding sites are s-alkylglutathione binding sites. | leukotriene c4 binding to mouse brain membranes was readily displaced by s-alkylglutathione derivatives, with the affinity of the test compound increasing as the alkyl chain length increases. s-decylglutathione was as potent as leukotriene c4. these data suggest that brain membrane leukotriene c4 binding sites are s-alkylglutathione binding sites, possibly microsomal glutathione transferase. | 1989 | 2542040 |
| sequences that promote formation of catenated intertwines during termination of dna replication. | the normal sequence at which sv40 dna replication terminates (ter) is unusual in that it promotes formation of catenated intertwines when two converging replication forks enter to complete replication (weaver et al., 1985). here we show that yeast centromeric sequences also exhibit this phenomenon. cen3 caused accumulation of late replicating intermediates and catenated dimers in plasmids replicating in mammalian cells, but only when it was located in the termination region (180 degrees from ori ... | 1989 | 2542894 |
| evaluation of assay systems for the detection of rotavirus in stool specimens. | we tested 41 rotavirus positive and 42 negative specimens as determined by electron microscopy. the assays systems used were an indirect nih-elisa, meritec-rotavirus, virogen rotatest, and rotazyme ii. meritec and virogen (latex agglutination assays) were the most sensitive tests, detecting 95% of the positive specimens. the nih-elisa detected 81% and rotazyme detected 63%. rotazyme was the most specific (100%), followed by the nih-elisa (95%) and the two latex agglutination systems (91%). to de ... | 1989 | 2546712 |
| stimulation of glucose transport in rat cardiac myocytes by guanosine 3',5'-monophosphate. | glucose transport in isolated rat cardiomyocytes is stimulated by insulin, catecholamines, and anoxia approximately 2- to 3-fold over basal rates. the molecular mechanisms controlling these responses are unknown. in our search for possible cellular mediators of glucose transport stimulation, we examined the effects of a number of nucleotides on 3-o-methylglucose transport in heart cells. the nucleotides and/or permeable analogs (monosuccinyl, 8-bromo, and dibutyryl derivatives) included cump, ci ... | 1989 | 2546735 |
| nitroimidazole derivatives inhibit anterior pituitary cell function apparently by a direct effect on the catalytic subunit of the adenylate cyclase holoenzyme. | nitroimidazole derivatives dose-dependently decreased basal and crf-stimulated acth release, basal and grf-stimulated rat gh release, and basal rat prl release in primary cultures of rat anterior pituitary cells. in addition, basal and crf-stimulated mrna coding for the acth precursor were reduced after preincubation with the nitroimidazole derivatives. miconazole, econazole, isoconazole, clotrimazole, and bifonazole had similar or more pronounced effects on anterior pituitary function compared ... | 1989 | 2546744 |
| human papillomavirus and cervical cancer. summary report. | 1989 | 2546883 | |
| stereological and functional investigations on isolated adrenocortical cells: zona fasciculata/reticularis cells of chronically acth-treated rats. | the morphology and function of isolated inner (zona fasciculata/reticularis) adrenocortical cells of rats pretreated with acth for 3, 6, 9 or 12 days were investigated. acth treatment induced a notable time-dependent enhancement in the steroidogenic capacity (corticosterone production) and growth of inner cells. the volumes of cells, mitochondrial compartment, membrane space [the cellular space occupied by smooth endoplasmic reticulum (ser) membranes] and lipid-droplet compartment, as well as th ... | 1989 | 2553263 |