Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| first isolation and characterization of edwardsiella tarda from diseased striped catfish, pangasianodon hypophthalmus (sauvage). | 2014 | 24344765 | |
| identification and characterization of lipopolysaccharide-induced tnf-alpha factor gene from japanese flounder paralichthys olivaceus. | lipopolysaccharide-induced tnf-α factor (litaf) is an important transcription factor participating in innate immunity through regulating tnf-α and other inflammatory cytokines expression. however, the expression and biological relevance of litaf in fish is still very limited. in this study, a full-length litaf cdna, termed politaf, was identified from japanese flounder paralichthys olivaceus. politaf contains a 67 bp 5'-untranslated sequence, a 435 bp open reading frame, and a 647 bp 3'-untransl ... | 2014 | 24359872 |
| molecular identification and expression analysis of a natural killer cell enhancing factor (nkef) from rock bream oplegnathus fasciatus and the biological activity of its recombinant protein. | natural killer cell enhancing factor (nkef) belongs to the defined peroxiredoxin (prx) family. rock bream nkef cdna was identified by expressed sequence tag (est) analysis of rock bream liver that was stimulated with the lps. the full-length rbnkef cdna (1062 bp) contained an open reading frame (orf) of 594 bp encoding 198 amino acids. rbnkef was significantly expressed in the gill, liver, and intestine. mrna expression of nkef in the head kidney was examined under viral and bacterial challenge ... | 2011 | 24371552 |
| development of a multiplex pcr assay to detect edwardsiella tarda, streptococcus parauberis, and streptococcus iniae in olive flounder (paralichthys olivaceus). | a multiplex pcr protocol was established to simultaneously detect major bacterial pathogens in olive flounder (paralichthys olivaceus) including edwardsiella (e.) tarda, streptococcus (s.) parauberis, and s. iniae. the pcr assay was able to detect 0.01 ng of e. tarda, 0.1 ng of s. parauberis, and 1 ng of s. iniae genomic dna. furthermore, this technique was found to have high specificity when tested with related bacterial species. this method represents a cheaper, faster, and reliable alternativ ... | 2014 | 24378589 |
| molecular cloning and expression analysis of two lipopolysaccharide-induced tnf-α factors (litafs) from rock bream, oplegnathus fasciatus. | lipopolysaccharide (lps)-induced tumor necrosis factor (tnf)-α factor (litaf) plays an important role controlling the expression of tnf-α and the other cytokine genes in the presence of lps. however, two litaf homologues have not been characterized in fish. in this study, we cloned two distinct litaf (rblitaf1 and rblitaf2) cdnas from rock bream (oplegnathus fasciatus) and characterized their expression profiles after infection with edwardsiella tarda, streptococcus iniae or red seabream iridovi ... | 2014 | 24394623 |
| dna vaccines: roles against diseases. | vaccination is the most successful application of immunological principles to human health. vaccine efficacy needs to be reviewed from time to time and its safety is an overriding consideration. dna vaccines offer simple yet effective means of inducing broad-based immunity. these vaccines work by allowing the expression of the microbial antigen inside host cells that take up the plasmid. these vaccines function by generating the desired antigen inside the cells, with the advantage that this may ... | 2013 | 24432284 |
| effect of edwardsiella tarda immunization on systemic immune response, mucosal immune response and protection in catla (catla catla). | the effect of immunization on systemic and cutaneous mucosal immune responses of fish and their possible relation with protection has not been fully assessed. in this study, healthy catla (catla catla) were immunized against edwardsiella tarda using two antigenic preparations namely, whole cell bacterin (b) and bacterin mixed with freund's complete adjuvant in a 1:1 (v/v) ratio (b+a) followed by a booster dose after 3 weeks of first injection. different systemic and cutaneous mucosal immune resp ... | 2014 | 24477841 |
| bacterial infections from aquatic species: potential for and prevention of contact zoonoses. | as aquaculture production and the consumption of aquaculture products increase, the possibility of contracting zoonotic infections from either handling or ingesting these products also increases. the principal pathogens acquired topically from fish or shellfish through spine/pincer puncture or open wounds are aeromonas hydrophila, edwardsiella tarda, mycobacterium marinum, streptococcus iniae, vibrio vulnificus and v. damsela. these pathogens, which are all indigenous to the aquatic environment, ... | 2013 | 24547653 |
| cloning and expression analyses of interferon regulatory factor (irf) 3 and 7 genes in european eel, anguilla anguilla with the identification of genes involved in ifn production. | interferon regulatory factor (irf) 3 and irf7 have been identified as regulators of type i interferon (ifn) gene expression in mammals. in the present study, the two genes were cloned and characterized in the european eel, anguilla anguilla. the full-length cdna sequence of irf3 and irf7 in the european eel, named as aairf3 and aairf7 consists of 2879 and 2419 bp respectively. multiple alignments showed that the two irfs have a highly conserved dna binding domain (dbd) in the n terminus, with th ... | 2014 | 24565894 |
| edwardsiella tarda hfq: impact on host infection and global protein expression. | hfq is an rna-binding protein that plays an important role in many cellular processes. in this study, we examined the biological effect of the hfq of edwardsiella tarda, a severe fish pathogen with a broad host range that includes humans. to facilitate the study, a markerless hfq in-frame deletion wild type, txhfq, was constructed. compared to the wild type tx01, txhfq exhibited (i) retarded planktonic and biofilm growth, (ii) decreased resistance against oxidative stress, (iii) attenuated overa ... | 2014 | 24568370 |
| in situ total aortic arch replacement for infected distal aortic arch aneurysms with penetrating atherosclerotic ulcer. | we present a series of patients who underwent in situ total aortic arch replacement for infected distal aortic arch aneurysms. | 2014 | 24607377 |
| eha, a transcriptional regulator of hemolytic activity of edwardsiella tarda. | hemolysis causes major symptoms such as the reddening skin and systemic hemorrhagic septicemia of diseased fish infected by edwardsiella tarda. cytolysin a (clya) is a pore-forming cytotoxic protein encoded by the clya gene in escherichia coli k-12. in this study, we observed that the heterologous expression of the eha gene from e. tarda could confer hemolytic activity upon a hemolytic-silent e. coli strain. the transcription of clya is positively controlled by the eha gene in e. tarda by rt-pcr ... | 2014 | 24628619 |
| a mutation in rcsb, a gene encoding the core component of the rcs cascade, enhances the virulence of edwardsiella tarda. | edwardsiella tarda, a gram-negative bacterium of the family enterobacteriaceae, is the causative agent of the systemic disease edwardsiellosis, which is a major problem in aquaculture industry worldwide. many virulence-related genes in e. tarda have been investigated, but the rcs phosphorelay, a two-component pathway, which regulates several cell-surface-associated structures related to invasion and survival in host cells, has not yet been thoroughly studied. in the present study, an rcsb in-fra ... | 2014 | 24631591 |
| impact of co-deficiency of rpon and rpos on stress tolerance, virulence and gene regulation in edwardsiella tarda. | edwardsiella tarda the etiological agent for edwardsiellosis, a devastating fish disease prevailing in worldwide aquaculture industries was subjected to a molecular genetic study. to research into the influence when rpon (σ(54) ) and rpos (σ(38) ) were deleted simultaneously, the double deletion mutant of rpon (σ(54) ) and rpos (σ(38) ), namely rnrs, was constructed. firstly, rpon and rpos are both essential for h2 o2 , starvation, high osmotic pressure and acid resistance, which have synergisti ... | 2014 | 24633758 |
| identification and expression analysis of lipopolysaccharide-induced tnf-alpha factor gene in chinese mitten crab eriocheir sinensis. | lipopolysaccharide-induced tnf-alpha factor (litaf) is an important transcription factor in transcriptional regulation of tnf-alpha and other cytokines. here we identified a full-length litaf homolog cdna, termed eslitaf, that contains a 71 bp 5'-untranslated sequence, an open reading frame consisting of 381 bp, and a 208 bp 3'-untranslated sequence in chinese mitten crab (eriocheir sinensis), an economically important freshwater crustacean species in china. eslitaf protein possesses a conserved ... | 2014 | 24657317 |
| in vitro quenching of fish pathogen edwardsiella tarda ahl production using marine bacterium tenacibaculum sp. strain 20j cell extracts. | quorum quenching (qq) has become an interesting alternative for solving the problem of bacterial antibiotic resistance, especially in the aquaculture industry, since many species of fish-pathogenic bacteria control their virulence factors through quorum sensing (qs) systems mediated by n-acylhomoserine lactones (ahls). in a screening for bacterial strains with qq activity in different marine environments, tenacibaculum sp. strain 20j was identified and selected for its high degradation activity ... | 2014 | 24695235 |
| complete genome sequence of the edwardsiella ictaluri-specific bacteriophage pei21, isolated from river water in japan. | we present the complete genome sequence for a novel edwardsiella ictaluri-specific bacteriophage, pei21, isolated from river water in japan. an initial comparative genome analysis revealed that the phage was closely related to the previously reported edwardsiella tarda phage msw-3 isolated from a red sea bream farm in japan. | 2014 | 24699953 |
| cell-penetrating peptides mediated protein cross-membrane delivery and its use in bacterial vector vaccine. | it is an attractive strategy to develop a recombinant bacterial vector vaccine by expressing exogenous protective antigen to induce the immune response, and the main concern is how to enhance the cellular internalization of antigen produced by bacterial vector. cell-penetrating peptides (cpps) are short cationic/amphipathic peptides which facilitate cellular uptake of various molecular cargoes and therefore have great potentials in vector vaccine design. in this work, eleven different cpps were ... | 2014 | 24746937 |
| multilocus variable number tandem repeat analysis of edwardsiella piscicida isolates pathogenic to fish. | this study describes a novel multilocus variable number tandem repeat analysis (mlva) based on six variable number of tandem repeat (vntr) loci for genotyping of 37 edwardsiella piscicida (previously edwardsiella tarda) isolates from multiple sources. the number of alleles identified for each of the six vntr loci ranged from 3 to 5 with vntr loci 1 (di = 0.632) and 3 (di = 0.644), displaying the highest degrees of polymorphism. mlva typing of the 37 e. piscicida isolates resulted in the identifi ... | 2014 | 24749655 |
| hu-induced polymorphous filamentation in fish pathogen edwardsiella tarda leading to reduced invasion and virulence in zebrafish. | edwardsiella tarda is a rod-shaped gram-negative pathogenic bacterium that causes hemorrhagic septicemia in fish. nucleoid-associated protein hu is a basic dna-binding protein with structural specificity in regulating genes expression. in wild-type e. tarda eib202, hu is composed of two subunits huα (hupa) and huβ (hupb), and exists in homodimer or heterodimer forms. different from the wild-type and δhupb mutant, δhupa mutant was found to be defective in cell growth, h2s production, acid adaptat ... | 2014 | 24793099 |
| molecular characterization and expression analysis of atp-gated p2x7 receptor involved in japanese flounder (paralichthys olivaceus) innate immune response. | atp-gated p2x7 receptor (p2rx7) channel is a key component for purinergic signaling and plays important roles in the innate immune response in mammals. however, the expression, molecular properties and immune significances of p2rx7 in lower vertebrates are still very limited. here we identified and characterized a novel bony fish p2rx7 homologue cdna, termed pop2rx7, in japanese flounder (paralichthys olivaceus). pop2rx7 protein shares about 60-88% sequence similarity and 45-78% sequence identit ... | 2014 | 24796752 |
| gc/ms-based metabolomics approach to identify biomarkers differentiating survivals from death in crucian carps infected by edwardsiella tarda. | microbial disease problems constitute the largest single cause of economic losses in aquaculture. an understanding of immune system in aquaculture animals how to function in defense against bacterial infections is especially important to control these diseases and improve food quality and safety. in the present study, we use a crucian carp model to explore which pathways and metabolites are crucial for the defense against infection caused by edwardsiella tarda eib202. we establish the metabolic ... | 2014 | 24837326 |
| expression and protective role of two novel nacht-containing proteins in pathogen infection. | lower vertebrates have been found to possess over 200 nacht-domain encoding genes; but, to date, very little is known about their functional activity. this article describes the sequences and expression analysis of two zebrafish nacht-containing proteins, namely nalpl1 and nalpl2. in addition, the functions of zebrafish nalpl1 and nalpl2, which are absent for both amino-terminal effector-binding domain (ebd) and carboxy-terminal ligand-recognition domain (lrd), were investigated for the first ti ... | 2014 | 24858030 |
| lack of a contact requirement for direct antibacterial activity of lymphocyte subpopulations in ginbuna crucian carp. | cytotoxic t lymphocytes (ctl) recognize and kill cells infected with viruses, intracellular bacteria and tumors with mhc restriction and antigen specificity. in addition to these activities, recent studies in mammals have suggested that ctl can exhibit direct microbicidal activity. in our previous study we documented direct antibacterial activity of cd4(+) t cells and sigm(+) cells as well as cd8α(+) t cells from immunized fish. however, we also found weak non-specific killing activity of lympho ... | 2014 | 24859594 |
| edwardsiella tarda-induced cytotoxicity depends on its type iii secretion system and flagellin. | many gram-negative bacteria utilize a type iii secretion system (t3ss) to translocate virulence proteins into host cells to cause diseases. in responding to infection, macrophages detect some of the translocated proteins to activate caspase-1-mediated cell death, called pyroptosis, and secretion of proinflammatory cytokines to control the infection. edwardsiella tarda is a gram-negative enteric pathogen that causes hemorrhagic septicemia in fish and both gastrointestinal and extraintestinal infe ... | 2014 | 24891103 |
| draft genome sequences of two genetic variant strains of edwardsiella piscicida, jf1305 and rsb1309, isolated from olive flounder (paralichythys olivaceus) and red sea bream (pagrus major) cultured in japan, respectively. | edwardsiella piscicida is a new species discovered within the group of organisms traditionally classified as edwardsiella tarda. we present draft genome sequences of two variant strains of e. piscicida, jf1305 and rsb1309. differences in protein-coding sequence between these isolates are associated with virulence, disease, and defense, suggesting differences in pathogenicity. | 2014 | 24926054 |
| preventive and therapeutic effects of auxotrophic edwardsiella tarda mutant harboring cpg 1668 motif-enriched plasmids against scuticociliatosis in olive flounder (paralichthys olivaceus). | previously generated two auxotrophic genes knockout edwardsiella tarda (δalr δasd e. tarda) was used as a delivery vehicle for cpg 1668 motif-enriched plasmids (pl-cpg30), and potential of the δalr δasd e. tarda-mediated delivery of cpg motifs in both prevention and therapeutic treatment of scuticociliatosis caused by miamiensis avidus in olive flounder (paralichthys olivaceus) was investigated. the auxotrophic e. tarda mutant with pl-cpg30 plasmids elicited evidently higher survival rates and d ... | 2014 | 24929150 |
| molecular aspects, genomic arrangement and immune responsive mrna expression profiles of two cxc chemokine receptor homologs (cxcr1 and cxcr2) from rock bream, oplegnathus fasciatus. | the cxcr1 and cxcr2 are the prototypical receptors and are the only known receptors for mammalian elr+ (glu-leu-arg) cxc chemokines, including cxcl8 (interleukin 8). these receptors transduce the elr+ chemokine signals and operate the downstream signaling pathways in inflammation and innate immunity. in this study, we report the identification and characterization of cxcr1 and cxcr2 genes from rock bream fish (ofcxcr1 and ofcxcr2) at the molecular level. the cdna and genomic dna sequences of the ... | 2014 | 24945570 |
| antimicrobial susceptibility and genetic characterisation of oxytetracycline-resistant edwardsiella tarda isolated from diseased eels. | edwardsiellosis is one of the most important bacterial diseases in eels. edwardsiella tarda (e. tarda) isolates (n=94) from diseased eels were screened against the eight most commonly used antimicrobial agents in aquaculture in taiwan. these isolates were highly susceptible to ampicillin, amoxicillin, florfenicol, oxolinic acid and flumequine. in total, 20 of the 94 (21.3 per cent) isolates tested were resistant to oxytetracycline (otc). among the 20 tetracycline-resistant e. tarda isolates, tet ... | 2014 | 24958553 |
| identification of a novel fish granzyme involved in cell-mediated immunity. | granzymes (gzms) are serine proteases released from cytoplasmic granules within cytotoxic t lymphocytes and natural killer (nk) cells. gzms induce apoptosis within virus-infected and transformed cells. in fish as well as mammals, gzms appear to play a major role in inducing target cell death. however, information on the function of fish gzms is limited, although gzm-like genes have been reported in several species. we identified and characterized a fish gzm (termed gcgzm) in ginbuna crucian carp ... | 2014 | 24968079 |
| calmodulin is a stress and immune response gene in chinese mitten crab eriocheir sinensis. | calmodulin (cam) is a multifunctional calcium sensor protein that participates in various cellular processes under normal, stress and pathological conditions. in crabs, however, the involvement of cam in response to environmental stress and immune challenges has not been revealed yet. in the present study, a cam cdna (escam) was identified from chinese mitten crab eriocheir sinensis and its mrna expression patterns in response to ambient (salinity and ph) stress and immune challenges was examine ... | 2014 | 24997436 |
| atp release channel pannexin1 is a novel immune response gene in japanese flounder paralichthys olivaceus. | extracellular atp is an important damage-associated molecular pattern molecule that plays key roles in innate immunity. in fish, however, the mechanism for extracellular atp release remains largely undefined. pannexin1 (panx1) is a newly discovered extracellular atp release channel with a wide tissue distribution and diverse biological functions in mammals. in the present study, we identified and characterized a panx1 homolog cdna, termed popanx1, from japanese flounder paralichthys olivaceus, w ... | 2014 | 25007779 |
| cloning, characterisation, and expression analysis of the cathepsin d gene from rock bream (oplegnathus fasciatus). | cathepsins are lysosomal cysteine proteases belonging to the papain family, members of which play important roles in normal metabolism for the maintenance of cellular homeostasis. rock bream (oplegnathus fasciatus) cathepsin d (rbctsd) cdnas were identified by expressed sequence tag analysis of a lipopolysaccharide-stimulated rock bream liver cdna library. the full-length rbctsd cdna (1644 bp) contained an open reading frame of 1191 bp encoding 396 amino acids. alignment analysis revealed that t ... | 2014 | 25038285 |
| effects of dietary scutellaria baicalensis extract on growth, feed utilization and challenge test of olive flounder (paralichthys olivaceus). | effects of dietary scutellaria baicalensis extract (sbe) on growth, feed utilization and challenge test of olive flounder (paralichthys olivaceus) were determined. six hundred thirty fish averaging 5.0 g were distributed into 18, 180-l tanks. six experimental diets were prepared in triplicate: sbe-0, sbe-0.5, sbe-1, sbe-2, sbe-3 and sbe-5 diets containing sbe at the concentrations of 0, 0.5, 1, 2, 3 and 5%, respectively. fish were hand-fed to apparent satiation twice a day for 8 wks. at the end ... | 2013 | 25049710 |
| expression analysis and biological activity of moronecidin from rock bream, oplegnathus fasciatus. | the piscidin-family, one of antimicrobial peptides (amps) mainly distributed in fish, is crucial effectors of fish innate immune response. piscidin-family typically has broad-spectrum antimicrobial activity and the ability to modulate the immune response. in this study, we identified moronecidin (rbmoro) included in piscidin-family from rock bream and investigated its gene expression using quantitative real-time pcr and biological activity (including antimicrobial and cytotoxic activity). the co ... | 2014 | 25050859 |
| interferon-α2b against microbes through promoting biosynthesis of unsaturated fatty acids. | interferon (ifn) family is a large group of cytokines involved in innate immune response against various microorganisms. however, whether ifn functions in antimicrobial property by metabolic pathways is largely unknown. in the present study, gc-ms-based metabolome is investigated in humoral fluid of zebrafish (danio rerio) which are exposed to three doses of ifn-α2b, designed as ifn-l, ifn-m, and ifn-h. out of 67 compounds identified, 19, 28, and 29 differential abundances of metabolites are ide ... | 2014 | 25058871 |
| a cdna microarray analysis to identify genes involved in the acute-phase response pathway of the olive flounder after infection with edwardsiella tarda. | the acute-phase response (apr) is an important systemic reaction that occurs within hours of an inflammatory signal caused by physical bodily injury or microbial infection. to investigate the apr of the olive flounder (paralichthys olivaceus) following infection with a pathogen, we established an expressed sequence tag (est)-based cdna microarray chip composed of 13,061 pcr-amplified cdnas encoding unique genes selected from an olive flounder est analysis. microarray analyses showed that the set ... | 2014 | 25063225 |
| emerging causes of superficial and invasive infections following marine injuries and exposures. | soft tissue bacterial infections following aquatic animal bites, stings, and minor injuries occur commonly and usually on the extremities in fishermen and beachgoers worldwide after freshwater and saltwater exposures. louisiana has more tidal, saltwater, and brackish water shorelines (more than 7,000 miles) than any other state, including alaska and hawaii. as a result, louisiana residents are often exposed to marine pathogens when fishing or working offshore or when enjoying louisiana's miles o ... | 2017 | 25075726 |
| [a comparison of methods for identifying mast cells of cyprinus carpio]. | to compare different identifying methods of the mast cells of red crucian carp (cyprinus carpio). | 2014 | 25108443 |
| cloning and expression analysis of three novel cc chemokine genes from japanese flounder (paralichthys olivaceus). | chemokines are small cytokines secreted by various cell types. they not only function in cell activation, differentiation and trafficking, but they also have influences on many biological processes. in this study, three novel cc chemokine genes paol-scya105, 106 and 107 in japanese flounder (paralichthys olivaceus) were cloned and characterized. paol-scya105 was mainly detected in gill, kidney and spleen, paol-scya106 was detected in all tissues examined and paol-scya107 was mainly detected in t ... | 2014 | 25123833 |
| a new target for the old regulator: h-ns suppress t6ss secretory protein evpp, the major virulence factor in the fish pathogen edwardsiella tarda. | the evpp gene in fish pathogen edwardsiella tarda, coding the t6ss secretory protein evpp and carrying an evpa-evpo independent promoter region, was crucial for host cell invasion. the transcription of evpp was positively regulated by either the two-component system esra-esrb or iron concentration, and its overexpression was known to enhance the invasion ability in our previous study. this work demonstrated that the h-ns protein, a pleiotropic regulator of gene expression, was a new transcriptio ... | 2014 | 25131176 |
| type iii secretion system genes of edwardsiella tarda associated with intracellular replication and virulence in zebrafish. | the type iii secretion system (t3ss) is essential for intracellular replication of edwardsiella tarda in phagocytes of fish and mammals. two possible effector candidate genes (esee and eseg) and 7 hypothetical genes (esab, escc, orf13, orf19, orf26, orf29, and orf30) located in the t3ss gene cluster were inactivated by an allelic exchange method, and we found that e. tarda strains carrying insertion mutations in escc, orf13, orf19, orf29, and orf30 were unable to replicate within j774 macrophage ... | 2014 | 25144115 |
| a live attenuated combination vaccine evokes effective immune-mediated protection against edwardsiella tarda and vibrio anguillarum. | edwardsiella tarda and vibrio anguillarum are the two main pathogenic bacteria that cause edwardsiellosis and vibriosis in various species of fish raised in aquaculture. in our previous study, the live attenuated vaccines e. tarda wed and v. anguillarum mvav6203 showed robust relative protection when vaccinated zebrafish or turbot were challenged with virulent e. tarda or v. anguillarum, respectively. additionally, vaccinated fish processed the two vaccines through different pathways of antigen ... | 2014 | 25219565 |
| role of intestinal inflammation in predisposition of edwardsiella tarda infection in zebrafish (danio rerio). | edwardsiella tarda, an enteric opportunistic pathogen, is associated with acute to chronic edwardsiellosis in cultured fish, resulting in heavy losses in aquaculture. to date, the pathogenesis of e. tarda has been extensively studied and a great deal of vaccine candidates have been attempted. however, the research on the predisposition of e. tarda infection is poorly reported. in this study, the effects of intestinal inflammation on e. tarda infection were investigated using a zebrafish model th ... | 2014 | 25224880 |
| molecular characterization and transcriptional analysis of non-mammalian type toll like receptor (tlr21) from rock bream (oplegnathus fasciatus). | toll-like receptors (tlrs) are a large family of pattern recognition receptors, which are involved in triggering host immune responses against various pathogens by detecting their evolutionarily conserved pathogen associated molecular patterns (pamps). tlr21 is a non-mammalian type tlr, which recognizes unmethylated cpg dna, and is considered as a functional homolog of mammalian tlr9. in this study, we attempted to identify and characterize a novel tlr21 counterpart from rock bream (oplegnathus ... | 2014 | 25300254 |
| comprehensive gene expression profiling in japanese flounder kidney after injection with two different formalin-killed pathogenic bacteria. | pathogenic bacteria possess some components, that are recognized by the host immune receptors. different components are recognized by distinct receptors, and thus it is speculated that these components may regulate different gene sets. to investigate the gene expression profiles regulated by different bacterial components in japanese flounder paralichthys olivaceus, japanese flounder were intraperitoneally injected with formalin-killed bacterial cells (fkc) of edwardsiella tarda and streptococcu ... | 2014 | 25304547 |
| concise synthesis of the tetrasaccharide repeating unit of the o-polysaccharide isolated from edwardsiella tarda pcm 1156 strain. | a convergent strategy has been developed for the synthesis of the tetrasaccharide repeating unit of the o-antigen from edwardsiella tarda pcm 1156. sequential glycosylations of a series of rationally protected monosaccharide intermediates were achieved either by the activation of thioglycosides using n-iodosuccinimide (nis) in conjunction with h2so4-silica or by activation of trichloroacetimidate by h2so4-silica only. all glycosylation reactions resulted in the formation of the desired linkage w ... | 2014 | 25369263 |
| infected hydatid cysts bacteria in slaughtered livestock and their effects on protoscoleces degeneration. | the protoscoleces of fertile hydatid cysts are considered as major risks in surgery and producing secondary cysts if rupture the cyst during operation and, cause infecting the dogs with adult worm if eaten by this animal. bacterial infection of the hydatid fluid can lead to sterilization of the cyst. | 2014 | 25371792 |
| aerobic bacterial microbiota isolated from the cloaca of the european pond turtle (emys orbicularis) in poland. | we conducted a comparative analysis of the aerobic cloacal bacteria of european pond turtles (emys orbicularis) living in their natural environment and juvenile turtles reared under controlled conditions in a breeding center. we included 130 turtles in the study. the aerobic bacteria isolated from the cloaca of the juvenile turtles were less diverse and more prevalent than the bacteria isolated from free-living adults. we isolated 17 bacterial species from juvenile captive turtles, among which t ... | 2015 | 25380369 |
| a disordered region in the evpp protein from the type vi secretion system of edwardsiella tarda is essential for evpc binding. | the type vi secretion system (t6ss) of pathogenic bacteria plays important roles in both virulence and inter-bacterial competitions. the effectors of t6ss are presumed to be transported either by attaching to the tip protein or by interacting with hcpi (haemolysin corregulated protein 1). in edwardsiella tarda ppd130/91, the t6ss secreted protein evpp (e. tarda virulent protein p) is found to be essential for virulence and directly interacts with evpc (hcp-like), suggesting that it could be a po ... | 2014 | 25401506 |
| edwardsiella tarda mlic, a lysozyme inhibitor that participates in pathogenesis in a manner that parallels ivy. | edwardsiella tarda, a bacterial pathogen to farmed fish as well as humans, possesses the genes of two lysozyme inhibitors, ivy and mlic (ivy(et) and mlic(et)). we recently studied ivyet and found it to be implicated in e. tarda virulence. in the present study, we characterized mlic(et) in comparison with ivy(et) in a turbot model. mlic(et) contains the fwskg motif and two cysteines (c33 and c98) that are highly conserved in subgroup 1 mlics but are of unknown functional importance. to examine th ... | 2014 | 25404031 |
| an invasive and low virulent edwardsiella tarda esrb mutant promising as live attenuated vaccine in aquaculture. | edwardsiella tarda is a leading fish pathogen haunting worldwide aquaculture industry. in e. tarda, two-component system esra-esrb positively regulates type iii and vi secretion systems (t3ss and t6ss) and negatively regulates hemolysin etha, which has been demonstrated to be essential for the invasion processes in fish. in order to develop a live attenuated vaccine (lav) with high invasiveness to be practically and economically used as immersion-administered vaccine in aquaculture, here, we gen ... | 2015 | 25431010 |
| a c-type lectin isolated from the skin of japanese bullhead shark (heterodontus japonicus) binds a remarkably broad range of sugars and induces blood coagulation. | the aim of this study was to determine the physiological role of skin lectins of the japanese bullhead shark (heterodontus japonicus). a skin extract was subjected to affinity chromatography using seven different sugars as ligands. molecular mass and n-terminal amino acid sequence analyses indicated elution of the same protein by each of the seven respective cognate ligands from sugar affinity columns. the predicted amino acid sequence encoded by the cdna of this protein [designated as h. japoni ... | 2015 | 25433861 |
| first molecular cloning and gene expression analysis of teleost cd42 (glycoprotein ib beta chain) gpib-ix-v subunit from rock bream, oplegnathus fasciatus. | cd42 is a platelet membrane glycoprotein ib that plays a key role in haemostasis and thrombin-induced platelet activation. here, we report the molecular cloning and sequence analysis of the cd42c gene from rock bream (oplegnathus fasciatus). rock bream cd42 (rbcd42c) gene expression profiles were determined after infection with streptococcus iniae, edwardsiella tarda and red seabream iridovirus (rsiv). the full-length rbcd42c cdna contained an open reading frame of 624 bp encoding 207 amino acid ... | 2015 | 25445900 |
| mavs splicing variants contribute to the induction of interferon and interferon-stimulated genes mediated by rig-i-like receptors. | the mitochondrial antiviral signaling protein (mavs) plays a key role in the signal transduction of rig-i-like receptors (rlrs)-mediated antiviral response. in the present study, zebrafish mavs transcript variants, namely mavs_tv1 and mavs_tv2, were cloned from zebrafish embryos. the putative mavs_tv1 protein (full length form) contains an n-terminal card domain, a central proline region, and a c-terminal transmembrane domain (tm). mavs_tv2 is generated by a 190 bp intron fragment insertion. the ... | 2015 | 25445907 |
| molecular genomic- and transcriptional-aspects of a teleost traf6 homolog: possible involvement in immune responses of oplegnathus fasciatus against pathogens. | tumor necrosis factor receptor (tnfr)-associated factor 6 (traf6) is a crucial docking molecule for tnfr superfamily and interleukin-1 receptor/toll-like receptor (il-1r/tlr) superfamily. as an adaptor protein in pathogen-induced signaling cascades, traf6 modulates both adaptive- and innate-immunity. in order to understand the immune responses of teleost traf6, oplegnathus fasciatus traf6-like gene (oftraf6) was identified and characterized. genomic length of oftraf6 (4 kb), obtained by means of ... | 2015 | 25449707 |
| two carboxypeptidase counterparts from rock bream (oplegnathus fasciatus): molecular characterization, genomic arrangement and immune responses upon pathogenic stresses. | carboxypeptidases (cps) are proteases that hydrolyze c-terminal peptide bonds. they are involved in regulating the complement system of the immune system. here, we report the molecular characterization and immune response of two carboxypeptidases, named carboxypeptidase a (rb-cpa) and carboxypeptidase n1 (rb-cpn1), from rock bream. the genomic sequence of rb-cpa contains 12 exons interrupted by 11 introns, while the genomic sequence of rb-cpn1 has 9 exons and 8 introns. the cdna sequence of rb-c ... | 2014 | 25457548 |
| two nf-κb inhibitor-alpha (iκbα) genes from rock bream (oplegnathus fasciatus): molecular characterization, genomic organization and mrna expression analysis after immune stimulation. | ikba is a member of ikb family, which sequesters nf-kb in an inactivate form in the cytoplasm and blocks the translocation of nf-kb to nucleus. the ikba paralogs of rock bream (ofikba-a and ofikba-b) encoded ikba proteins with typical features including, highly conserved ikb degradation motif, six ankyrin repeats and a pest sequence. however, their amino acid identity and similarity were only 55.6 and 69.7%, respectively suggesting that these two genes could be the two different isoforms of ikba ... | 2014 | 25462458 |
| phylogenomics characterization of a highly virulent edwardsiella strain et080813(t) encoding two distinct t3ss and three t6ss gene clusters: propose a novel species as edwardsiella anguillarum sp. nov. | as important zoonotic organisms causing infections in humans, edwardsiella bacteria are also notorious leading fish pathogens haunting worldwide aquaculture industries. however, the taxa are now widely recognized to be misclassified, which hurdled the understanding of the epidemiology and development of effective diagnostics and vaccines. currently the genus edwardsiella consists of three species edwardsiella tarda, e. ictaluri, and e. hoshinae. previous phylogenomic analysis revealed that e. ta ... | 2015 | 25466920 |
| enterobacteria identification and detection of diarrheagenic escherichia coli in a port complex. | the port complex of maranhão (pcm) is the second largest port complex in brazil, receiving ships with large volumes of ballast water. to evaluate the microbiological quality of its waters, physicochemical parameters (ph and salinity), the number of coliforms (thermotolerants and totals), and the presence of enterobacterias and diarrheagenic escherichia coli strains were analyzed. in order to identify the presence of e. coli virulence genes target regions of the stx, elt, est, aggr, cvd432, ipah ... | 2014 | 25477930 |
| adhesive and invasive capacities of edwardsiella tarda isolated from south american sea lion. | edwarsiella tarda is a zoonotic bacterium that can be isolated from humans, animals and the environment. although e. tarda is primarily considered a fish pathogen, it is the only species of its genus considered to be pathogenic for humans as well. a survey of zoonotic intestinal bacteria in fresh feces from south american sea lions (sasl) otaria flavescens, reported e. tarda as the most frequently isolated species. in this study, we used hep-2 cells to establish in vitro the adherence and invasi ... | 2014 | 25477948 |
| higher antiviral response of rig-i through enhancing rig-i/mavs-mediated signaling by its long insertion variant in zebrafish. | as an intracellular pattern recognition receptor (prr), the retinoic acid-inducible gene-i (rig-i) is responsible for the recognition of cytosolic viral nucleic acids and the production of type i interferons (ifns). in the present study, an insertion variant of rig-i with 38 amino acids inserted in the n-terminal card2 domain, as well as the typical type, named as rig-ia and rig-ib respectively were identified in zebrafish. rig-ia and rig-ib were all up-regulated following the infection of a neg ... | 2015 | 25524497 |
| generation and evaluation of virulence attenuated mutants of edwardsiella tarda as vaccine candidates to combat edwardsiellosis in flounder (paralichthys olivaceus). | edwardsiella tarda is an intracellular pathogen that causes edwardsiellosis in fish. the development of a live attenuated vaccine may be an effective approach for preventing this disease in fish. in this study, we introduced deletions of esrb, esac, evph, rpos, and pura into the e. tarda lse40δaroa strain, thereby generating five double-gene mutants (δaroaδesrb, δaroaδesac, δaroaδrpos, δaroaδevph, and δaroaδpura) and two triple-gene mutants (δaroaδesrbδevph and δaroaδesacδevph). when blue gouram ... | 2015 | 25541077 |
| identification and characterization of the cdnas encoding the two subunits of chinese mitten crab (eriocheir sinensis) calcineurin: their implications in stress and immune response. | calcineurin (cn), the only ca(2+)/calmodulin-activated serine/threonine protein phosphatase, is a key effector participating in ca(2+)-dependent signal transduction pathways in a number of cellular processes under normal, stress and pathological conditions. however, the expression and the relevance of cn in stress and immune response have not been characterized in crustaceans. here, we identified the cdnas that encode the two subunits of cn (termed escn-a and escn-b, respectively) in chinese mit ... | 2015 | 25541080 |
| mitochondrial peroxiredoxin 3 (prx3) from rock bream (oplegnathus fasciatus): immune responses and role of recombinant prx3 in protecting cells from hydrogen peroxide induced oxidative stress. | pathogenic infections and environmental factors cause a variety of stresses in fish including oxidative stress by rapid elevation of reactive oxygen species (ros) and reactive nitrogen species (rns). transcriptional activation and expression of antioxidant enzymes are essential for reducing the oxidative stress. in this study, we present the molecular characterization, immune responses and ros scavenging activity of mitochondrial peroxiredoxin 3 from oplegnathus fasciatus (rbprx3). coding sequen ... | 2015 | 25542382 |
| insights into molecular profiles and genomic evolution of an irak4 homolog from rock bream (oplegnathus fasciatus): immunogen- and pathogen-induced transcriptional expression. | as a pivotal signaling mediator of toll-like receptor (tlr) and interleukin (il)-1 receptor (il-1r) signaling cascades, the il-1r-associated kinase 4 (irak4) is engaged in the activation of host immunity. this study investigates the molecular and expressional profiles of an irak4-like homolog from oplegnathus fasciatus (ofirak4). the ofirak4 gene (8.2 kb) was structured with eleven exons and ten introns. a putative coding sequence (1395bp) was translated to the ofirak protein of 464 amino acids. ... | 2015 | 25555811 |
| edwardsiella tarda evades serum killing by preventing complement activation via the alternative pathway. | edwardsiella tarda is a gram-negative bacterium with a broad host range that includes a wide variety of farmed fish as well as humans. e. tarda has long been known to be able to survive in host serum, but the relevant mechanism is unclear. in this study, we investigated the fundamental question, i.e. whether e. tarda activated serum complement or not. we found that (i) when incubated with flounder serum, e. tarda exhibited a high survival rate (87.6%), which was slightly but significantly reduce ... | 2015 | 25575477 |
| characterization of ampicillin-stressed proteomics and development of a direct method for detecting drug-binding proteins in edwardsiella tarda. | antibiotic-resistant edwardsiella tarda poses a severe challenge to aquaculture. an understanding for antibiotic-resistant mechanisms is crucial to control the disease. the present study characterizes e. tarda ampicillin-stressed proteome and shows the importance of energy metabolism including the tca cycle and glycolysis/gluconeogenesis in the antibiotic resistance. further combination with antibiotic measurement develops a new method for identification of antibiotic-binding proteins out of dif ... | 2015 | 25596334 |
| real-time polymerase chain reaction assays for the detection and quantification of edwardsiella tarda, edwardsiella piscicida, and edwardsiella piscicida-like species in catfish tissues and pond water. | researchers have proposed the adoption of 3 distinct genetic taxa among bacteria previously classified as edwardsiella tarda; namely e. tarda, e. piscicida, and a taxon presently termed e. piscicida-like. individual real-time polymerase chain reaction (qpcr) assays were developed, based on published primers, for e. tarda, e. piscicida, and e. piscicida-like sp. to provide rapid quantitative confirmatory tests for these phenotypically ambiguous bacteria. the qpcr assays were shown to be repeatabl ... | 2015 | 25613040 |
| exogenous alanine and/or glucose plus kanamycin kills antibiotic-resistant bacteria. | multidrug-resistant bacteria are an increasingly serious threat to human and animal health. however, novel drugs that can manage infections by multidrug-resistant bacteria have proved elusive. here we show that glucose and alanine abundances are greatly suppressed in kanamycin-resistant edwardsiella tarda by gc-ms-based metabolomics. exogenous alanine or glucose restores susceptibility of multidrug-resistant e. tarda to killing by kanamycin, demonstrating an approach to killing multidrug-resista ... | 2015 | 25651179 |
| identification and functional characterization of the novel edwardsiella tarda effector esej. | edwardsiella tarda is a gram-negative enteric pathogen that causes hemorrhagic septicemia in fish and gastro- and extraintestinal infections in humans. the type iii secretion system (t3ss) of e. tarda has been identified as a key virulence factor that contributes to pathogenesis in fish. however, little is known about the associated effectors translocated by this t3ss. in this study, by comparing the profile of secreted proteins of the wild-type ppd130/91 and its t3ss atpase δesan mutant, we ide ... | 2015 | 25667268 |
| fructose restores susceptibility of multidrug-resistant edwardsiella tarda to kanamycin. | edwardsiella tarda, the causative agent of edwardsiellosis, imposes medical challenges in both the clinic and aquaculture. the emergence of multidrug resistant strains makes antibiotic treatment impractical. the identification of molecules that facilitate or promote antibiotic efficacy is in high demand. in the present study, we aimed to identify small molecules whose abundance is correlated with kanamycin resistance in e. tarda by gas chromatography-mass spectrometry. we found that the abundanc ... | 2015 | 25675328 |
| a quorum sensing-based in vivo expression system and its application in multivalent bacterial vaccine. | delivery of antigens by live bacterial carriers can elicit effective humoral and cellular responses and may be an attractive strategy for live bacterial vaccine production through introduction of a vector that expresses an exogenous protective antigen. to overcome the instability and metabolic burden associated with plasmid introduction, alternative strategies, such as the use of in vivo-inducible promoters, have been proposed. however, screening an ideal in vivo-activated promoter with high eff ... | 2015 | 25888727 |
| edwardsiella tarda-regulated proteins in japanese flounder (paralichthys olivaceus): identification and evaluation of antibacterial potentials. | edwardsiella tarda is a severe aquaculture pathogen that infects a wide range of fish including japanese flounder (paralichthys olivaceus). in this study, proteomic analysis was conducted to examine flounder spleen proteins altered in expression during e. tarda infection. twenty differentially expressed proteins were identified, which belonged to five functional categories. five upregulated proteins, i.e. calmodulin (cam), cathepsin l (catl), calreticulin (crt), ferritin middle subunit (ferm), a ... | 2015 | 25896741 |
| edwardsiella tarda sip1: a serum-induced zinc metalloprotease that is essential to serum resistance and host infection. | edwardsiella tarda is a severe bacterial pathogen to a wide arrange of farmed fish. one salient virulent feature of e. tarda is a remarkable ability to survive in host serum. in this study, in order to identify e. tarda proteins involved in serum resistance, we conducted proteomic analysis to examine the extracellular protein profiles of tx01, a pathogenic e. tarda isolate, in response to serum treatment. five differentially expressed proteins were identified, one of which was a putative zinc pr ... | 2015 | 25899393 |
| functional characterisation and expression analysis of recombinant serum amyloid p isoform 1 (rbsap1) from rock bream (oplegnathus fasciatus). | lectins are carbohydrate-binding proteins that play important roles in the recognition and elimination of pathogens via the innate immune system. pentraxins (ptx) are humoral lectins, which are multifunctional proteins in vertebrates. pentraxins can be divided into two groups based on their primary structure: short (c-reactive protein and serum amyloid p [sap]) and long pentraxins (ptx3 and neuronal pentraxins). previously, sap was shown to have ca(2+)-dependent binding specificity for various l ... | 2015 | 25917975 |
| a potential antioxidant enzyme belonging to the atypical 2-cys peroxiredoxin subfamily characterized from rock bream, oplegnathus fasciatus. | peroxiredoxins (prxs), a diverse family of antioxidant enzymes, exert their antioxidant function through which different peroxide species are detoxified. this study describes both structural and functional characterization of a mitochondrial prx identified in rock bream, oplegnathus fasciatus (rbprx5). the orf (573 bp) of rbprx5 encoded a protein of 190 amino acids (20 kda) containing a putative mitochondrial targeting sequence (residues 1-20) and a thioredoxin-2 motif (residues 31-190) and thre ... | 2015 | 25934084 |
| first molecular characterisation and expression analysis of a teleost thioredoxin-interacting protein (txnip) gene from rock bream (oplegnathus fasciatus). | thioredoxin-interacting protein (txnip) is an important regulator of glucose metabolism that functions by inhibiting cellular glucose uptake. the full-length rock bream (oplegnathus fasciatus) txnip (rbtxnip) cdna (2499 bp) contains an open reading frame of 1188 bp encoding 396 amino acids. furthermore, multiple alignments showed that the arrestin domain was well conserved among the other txnip sequences tested. rbtxnip was predicted to contain a pxxp and ppxy motif. phylogenetic analysis indica ... | 2015 | 25934185 |
| comparative analysis of adaptive immune response after vaccine trials using live attenuated and formalin-killed cells of edwardsiella tarda in ginbuna crucian carp (carassius auratus langsdorfii). | edwardsiella tarda is an intracellular pathogen that causes edwardsiellosis in fish. although vaccine trials with formalin-killed cells (fkc) have been reported, the vaccinations failed in protect against e. tarda infection. on the other hand, a live attenuated vaccine strategy is effective against edwardsiellosis; however, the mechanism underlying its effectiveness in fish is unclear. in the present study, we compared the adaptive immune responses in fish vaccinated with fkcs and live attenuate ... | 2015 | 25959574 |
| comparative proteomic study of edwardsiella tarda strains with different degrees of virulence. | edwardsiella tarda is an enteric opportunistic pathogen that causes a great loss in aquaculture. this species has been described as a phenotypical homogeneous group; in contrast, serological studies and molecular typing revealed a wide heterogeneity. in this work, a proteomic study of differential expression of a virulent isolate from turbot cultured in the norwest of spain in comparison with an avirulent collection strain was performed in order to recognize proteins involved in virulence. one h ... | 2015 | 25979771 |
| in vivo programmed gene expression based on artificial quorum networks. | the quorum sensing (qs) system, as a well-functioning population-dependent gene switch, has been widely applied in many gene circuits in synthetic biology. in our work, an efficient cell density-controlled expression system (qs) was established via engineering of the vibrio fischeri luxi-luxr quorum sensing system. in order to achieve in vivo programmed gene expression, a synthetic binary regulation circuit (araqs) was constructed by assembling multiple genetic components, including the quorum q ... | 2015 | 25979894 |
| full-genome sequence of a novel myovirus, gf-2, infecting edwardsiella tarda: comparison with other edwardsiella myoviral genomes. | edwardsiellosis, which is caused by edwardsiella tarda, a gram-negative bacterium, is one of the most serious infectious diseases in both marine and freshwater fish farms worldwide. previously, we reported the complete genome sequences of three e. tarda-lytic bacteriophages (two podoviruses and a myovirus), which were isolated from fish tissues and fish-rearing seawater. further genomic information regarding e. tarda phages is important for understanding phage-host interactions as well as for ap ... | 2015 | 26049743 |
| ontogeny, tissue distribution and expression analysis of igz in rohu, labeo rohita in response to various stimuli. | this study reports presence of igz transcripts, its ontogeny, tissue distribution and expression following various stimulations/infections in rohu. the derived rohu igz sequence clustered together with igz of ctenopharyngodon idella and cyprinus carpio in phylogenetic analysis. igz expression was detected at early developmental stages with higher expression at 1 day post-fertilization, and higher in anterior kidney and lower in skin tissues of juveniles. an inductive expression of igz was observ ... | 2015 | 26055084 |
| metabolome strategy against edwardsiella tarda infection through glucose-enhanced metabolic modulation in tilapias. | edwardsiella tarda causes fish disease and great economic loss. however, metabolic strategy against the pathogen remains unexplored. in the present study, gc-ms based metabolomics was used to investigate the metabolic profile from tilapias infected by sublethal dose of e. tarda. the metabolic differences between the dying group and survival group allow the identification of key pathways and crucial metabolites during infections. more importantly, those metabolites may modulate the survival-relat ... | 2015 | 26057462 |
| generation of killed but metabolically active (kbma) edwardsiella tarda and evaluation of its potential as a protective vaccine. | a technology for inactivation of pathogens in human blood products by treatment with amotosalen hydrochloride (s-59) in combination with long wavelength ultraviolet light (uva) to decrease transfusion-mediated sepsis has been applied to make safe vaccines against human pathogenic bacteria, and the resultants were called killed but metabolically active (kbma) bacteria. in the present study, we first generated kbma edwardsiella tarda and evaluated its potential as a protective vaccine in olive flo ... | 2015 | 26074095 |
| effects of protein hydrolysates supplementation in low fish meal diets on growth performance, innate immunity and disease resistance of red sea bream pagrus major. | this study was conducted to evaluate the supplemental effects of three different types of protein hydrolysates in a low fish meal (fm) diet on growth performance, feed utilization, intestinal morphology, innate immunity and disease resistance of juvenile red sea bream. a fm-based diet was used as a high fish meal diet (hfm) and a low fish meal (lfm) diet was prepared by replacing 50% of fm by soy protein concentrate. three other diets were prepared by supplementing shrimp, tilapia or krill hydro ... | 2015 | 26074096 |
| insights into the virulence-related genes of edwardsiella tarda isolated from turbot in europe: genetic homogeneity and evidence for vibrioferrin production. | edwardsiella tarda has long been known as a pathogen that causes severe economic losses in aquaculture industry. insights gained on e. tarda pathogenesis may prove useful in the development of new methods for the treatment of infections as well as preventive measures against future outbreaks. in this report, we have established the correlation between the presence of virulence genes, related with three aspects typically involved in bacterial pathogenesis (chondroitinase activity, quorum sensing ... | 2016 | 26096159 |
| complete genome sequence of edwardsiella tarda isolate fl95-01, recovered from channel catfish. | edwardsiella tarda is a gram-negative facultative anaerobe that has been isolated from fish, reptiles, amphibians, and mammals, including humans. this is a report of the complete and annotated genome of isolate fl95-01, recovered from channel catfish (ictalurus punctatus). | 2015 | 26112788 |
| type iii secretion system translocon component eseb forms filaments on and mediates autoaggregation of and biofilm formation by edwardsiella tarda. | the type iii secretion system (t3ss) of edwardsiella tarda plays an important role in infection by translocating effector proteins into host cells. eseb, a component required for effector translocation, is reported to mediate autoaggregation of e. tarda. in this study, we demonstrate that eseb forms filamentous appendages on the surface of e. tarda and is required for biofilm formation by e. tarda in dulbecco's modified eagle's medium (dmem). biofilm formation by e. tarda in dmem does not requir ... | 2015 | 26116669 |
| identification and expression analysis of nascent polypeptide-associated complex alpha gene in response to immune challenges in japanese flounder paralichthys olivaceus. | nascent polypeptide-associated complex (nac) is a conserved heterodimeric protein consisting of alpha and beta subunits. in addition to acting as a protein translation chaperone by forming a heterodimer with the beta subunit, nac alpha (naca) also shows important immune significance independent of nac beta in mammalian cells. in lower vertebrates, however, the immunological relevance of naca has not been revealed yet. in the present study, we identified and characterized a naca gene (termed pona ... | 2015 | 26142144 |
| a homolog of kunitz-type serine protease inhibitor from rock bream, oplegnathus fasciatus: molecular insights and transcriptional modulation in response to microbial and pamp stimulation, and tissue injury. | serine proteases and their inhibitors play vital roles in diverse biological processes. in this study, we identified and characterized cdna coding for a kunitz-type serine protease inhibitor (spi), which we designated as rbkspi, in a commercially important species, rock bream. the full-length cdna sequence of rbkspi consisted of 2452 bp with an open reading frame (orf) of 1521 bp encoding a polypeptide of 507 amino acid (aa) residues. in the rbkspi protein, manec, pkd, ldla, and two kunitz domai ... | 2015 | 26162478 |
| immune response of flounder (paralichthys olivaceus) was associated with the concentration of inactivated edwardsiella tarda and immersion time. | in the optimization of immersion strategy, vaccine concentration and immersion time are two key factors needed to be considered, which largely determined the immune efficacy. in this work, the healthy flounder were vaccinated in formalin-killed edwardsiella tarda at four concentrations (10(6), 10(7), 10(8), 10(9) cfu ml(-1)) for three immersion times (30, 60, 90 min), respectively. at the 6th week post vaccination, the flounders were challenged with live e. tarda, and the relative percent surviv ... | 2015 | 26163937 |
| endoscopic ultrasonography-guided transmural drainage of an infected hepatic cyst due to edwardsiella tarda: a case report. | infected hepatic cysts are very rare compared to simple liver cysts and abscesses. we treated a 77-year-old man with an infected hepatic cyst in the lateral segment caused by edwardsiella tarda, which has not been previously reported as a pathogenic organism associated with infected hepatic cysts. percutaneous drainage was temporarily effective, but infection recurred after the drainage tube was removed. we then inserted two drainage tubes into the cyst using an endoscopic ultrasonography (eus)- ... | 2014 | 26184023 |
| starvation beneficially influences the liver physiology and nutrient metabolism in edwardsiella tarda infected red sea bream (pagrus major). | dietary compromises, especially food restrictions, possess species-specific effects on the health status and infection control in several organisms, including fish. to understand the starvation-mediated physiological responses in edwardsiella tarda infected red sea bream, especially in the liver, we performed a 20-day starvation experiment using 4 treatment (2 fed and 2 starved) groups, namely, fed-placebo, starved-placebo, fed-infected, and starved-infected, wherein bacterial exposure was done ... | 2015 | 26188170 |
| first report of edwardsiellosis in cage-cultured sharpsnout sea bream, diplodus puntazzo from the mediterranean. | edwardsiella tarda, is a serious bacterial pathogen affecting a broad range of aquaculture fish species. the bacterium has also been reported as a human pathogen, however recent studies have dissociated the fish pathogenic edwardsiella from those isolated from humans by placing them in a new species, e. piscicida. here we report the first case of edwardsiellosis in cultured sharpsnout sea breams, diplodus puntazzo in greece. | 2015 | 26193880 |
| characterization of rock bream (oplegnathus fasciatus) complement components c1r and c1s in terms of molecular aspects, genomic modulation, and immune responsive transcriptional profiles following bacterial and viral pathogen exposure. | the complement components c1r and c1s play a crucial role in innate immunity via activation of the classical complement cascade system. as initiators of the pathogen-induced signaling cascade, c1r and c1s modulate innate immunity. in order to understand the immune responses of teleost c1r and c1s, oplegnathus fasciatus c1r and c1s genes (ofc1r and ofc1s) were identified and characterized. the genomic sequence of ofc1r was enclosed with thirteen exons that represented a putative peptide with 704 ... | 2015 | 26241508 |
| recombinant sialidase nana (rnana) cleaves α2-3 linked sialic acid of host cell surface n-linked glycoprotein to promote edwardsiella tarda infection. | edwardsiella tarda is one of the major pathogenic bacteria affecting both marine and freshwater fish species. sialidase nana expressed endogenously in e. tarda is glycosidase removing sialic acids from glycoconjugates. recently, the relationship of nana sialidase activity to e. tarda infection has been reported, however, the mechanism with which sialidase nana aids the pathogenicity of e. tarda remained unclear. here, we comprehensively determined the biochemical properties of nana towards vario ... | 2015 | 26291491 |
| roles of rpos in yersinia pseudotuberculosis stress survival, motility, biofilm formation and type vi secretion system expression. | rpos (σ(s)), the stationary phase/stress σ factor, controls the expression of a large number of genes involved in cellular responses to a variety of stresses. however, the role of rpos appears to differ in different bacteria. while rpos is an important regulator of flagellum biosynthesis, it is associated with biofilm development in edwardsiella tarda. biofilms are dense communities formed by bacteria and are important for microbe survival under unfavorable conditions. the type vi secretion syst ... | 2015 | 26310305 |
| enhanced immune response and resistance to edwardsiellosis following dietary chitooligosaccharide supplementation in the olive flounder (paralichthys olivaceus). | this study was conducted to evaluate the effects of dietary chitooligosaccharide (cos) supplementation on peripheral leukocyte count, head kidney leukocyte phagocytic rate, phagocytic index, respiratory burst activity, serum lysozyme activity, and immune protection in paralichthys olivaceus. a total of 300 flounder with an average body weight of 80-100 g were randomly assigned into four dietary groups: (i) basic diet (control), basic diet containing (ii) 0.5% cos, and (iii) 1% cos, fed continuou ... | 2015 | 26314523 |
| molecular characterization of purinergic receptor p2x4 involved in japanese flounder (paralichthys olivaceus) innate immune response and its interaction with atp release channel pannexin1. | p2x4 receptor (p2x4r) is a member of trimeric atp-gated receptor channel family. despite the importance of p2x4r in innate immunity has been addressed in mammals, the immunological significance of p2x4r has not been characterized in fish. in the present study we identified a full-length p2x4r cdna sequence from japanese flounder paralichthys olivaceus (termed pop2x4r) by rt-pcr and race approaches and analyzed its gene expression patterns under normal and immune challenge conditions. qualitative ... | 2015 | 26321132 |