Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| transcriptome analysis of a phenol-producing pseudomonas putida s12 construct: genetic and physiological basis for improved production. | the unknown genetic basis for improved phenol production by a recombinant pseudomonas putida s12 derivative bearing the tpl (tyrosine-phenol lyase) gene was investigated via comparative transcriptomics, nucleotide sequence analysis, and targeted gene disruption. we show upregulation of tyrosine biosynthetic genes and possibly decreased biosynthesis of tryptophan caused by a mutation in the trpe gene as the genetic basis for the enhanced phenol production. in addition, several genes in degradatio ... | 2008 | 17993537 |
| microbial degradation of phosmet on blueberry fruit and in aqueous systems by indigenous bacterial flora on lowbush blueberries (vaccinium angustifolium). | phosmet-adapted bacteria isolated from lowbush blueberries (vaccinium angustifolium) were evaluated for their ability to degrade phosmet on blueberry fruit and in minimal salt solutions. microbial metabolism of phosmet by isolates of enterobacter agglomerans and pseudomonas fluorescens resulted in significant reductions (p < 0.05; 33.8%) in phosmet residues on blueberry fruit. degradation was accompanied by microbial proliferation of phosmet-adapted bacteria. preferential utilization of phosmet ... | 2007 | 17995608 |
| virulence mechanisms and host specificity of gall-forming pantoea agglomerans. | pantoea agglomerans has been transformed from a commensal bacterium associated with many plants into a host-specific gall-forming pathogen by acquiring a plasmid-borne pathogenicity island. this pathogenicity island harbors the hrp/hrc gene cluster, in addition to genes encoding type iii effector proteins, biosynthesis of the phytohormones indole-3-acetic acid and cytokinin, multiple diverse insertion sequences and pseudogenes. this review describes a unique model for understanding the emergence ... | 2007 | 18024130 |
| the structure of the o-specific polysaccharide of the lipopolysaccharide from pantoea agglomerans strain fl1. | a neutral o-specific polysaccharide consisting of d-rhamnose was obtained by mild acid hydrolysis of the lipopolysaccharide of the plant pathogenic bacterium pantoea agglomerans strain fl1, a common epiphyte of many plant species, and associated with pseudomonas savastanoi pv. savastanoi in young and apparently intact olive knots. by means of compositional and methylation analyses, and nmr spectroscopy, the chemical repeating unit of the polymer was identified as a linear tetrasaccharide of the ... | 2008 | 18039532 |
| enterobacter sakazakii invasion in human intestinal caco-2 cells requires the host cell cytoskeleton and is enhanced by disruption of tight junction. | enterobacter sakazakii is an opportunistic pathogen that causes systemic bacteremia and meningitis with high mortality, and powdered infant formula is a frequent source of this bacterium. however, the mechanisms that this organism uses to invade and translocate through the intestinal barrier are unknown. using caco-2 epithelial cells, we were able to demonstrate penetration of e. sakazakii and to determine invasion-associated properties. we found that e. sakazakii entry and invasion were depende ... | 2008 | 18070906 |
| enterobacter sakazakii invasion in human intestinal caco-2 cells requires the host cell cytoskeleton and is enhanced by disruption of tight junction. | enterobacter sakazakii is an opportunistic pathogen that causes systemic bacteremia and meningitis with high mortality, and powdered infant formula is a frequent source of this bacterium. however, the mechanisms that this organism uses to invade and translocate through the intestinal barrier are unknown. using caco-2 epithelial cells, we were able to demonstrate penetration of e. sakazakii and to determine invasion-associated properties. we found that e. sakazakii entry and invasion were depende ... | 2008 | 18070906 |
| distribution of conjugative-plasmid-mediated 16s rrna methylase genes among amikacin-resistant enterobacteriaceae isolates collected in 1995 to 1998 and 2001 to 2006 at a university hospital in south korea and identification of conjugative plasmids mediating dissemination of 16s rrna methylase. | the distribution of conjugative-plasmid-mediated 16s rrna methylase genes among amikacin-resistant enterobacteriaceae collected between 1995 and 1998 and between 2001 and 2006 at a university hospital in south korea was examined, and conjugative plasmids carrying the 16s rrna methylase genes were characterized by pcr-based replicon typing and by determination of their antimicrobial resistance pattern. among the 7,127 isolates, 463 isolates showed a high level of resistance to amikacin, and 218 o ... | 2008 | 18094126 |
| distribution of conjugative-plasmid-mediated 16s rrna methylase genes among amikacin-resistant enterobacteriaceae isolates collected in 1995 to 1998 and 2001 to 2006 at a university hospital in south korea and identification of conjugative plasmids mediating dissemination of 16s rrna methylase. | the distribution of conjugative-plasmid-mediated 16s rrna methylase genes among amikacin-resistant enterobacteriaceae collected between 1995 and 1998 and between 2001 and 2006 at a university hospital in south korea was examined, and conjugative plasmids carrying the 16s rrna methylase genes were characterized by pcr-based replicon typing and by determination of their antimicrobial resistance pattern. among the 7,127 isolates, 463 isolates showed a high level of resistance to amikacin, and 218 o ... | 2008 | 18094126 |
| multiorgan failure and rhabdomyolysis in a recent émigré: your diagnosis? | 2000 | 18159279 | |
| detection of plant-modulated alterations in antifungal gene expression in pseudomonas fluorescens cha0 on roots by flow cytometry. | the biocontrol activity of the root-colonizing pseudomonas fluorescens strain cha0 is largely determined by the production of antifungal metabolites, especially 2,4-diacetylphloroglucinol. the expression of these metabolites depends on abiotic and biotic environmental factors, in particular, elements present in the rhizosphere. in this study, we have developed a new method for the in situ analysis of antifungal gene expression using flow cytometry combined with green fluorescent protein (gfp)-ba ... | 2008 | 18165366 |
| detection of plant-modulated alterations in antifungal gene expression in pseudomonas fluorescens cha0 on roots by flow cytometry. | the biocontrol activity of the root-colonizing pseudomonas fluorescens strain cha0 is largely determined by the production of antifungal metabolites, especially 2,4-diacetylphloroglucinol. the expression of these metabolites depends on abiotic and biotic environmental factors, in particular, elements present in the rhizosphere. in this study, we have developed a new method for the in situ analysis of antifungal gene expression using flow cytometry combined with green fluorescent protein (gfp)-ba ... | 2008 | 18165366 |
| structures of open (r) and close (t) states of prephenate dehydratase (pdt)--implication of allosteric regulation by l-phenylalanine. | the enzyme prephenate dehydratase (pdt) converts prephenate to phenylpyruvate in l-phenylalanine biosynthesis. pdt is allosterically regulated by l-phe and other amino acids. we report the first crystal structures of pdt from staphylococcus aureus in a relaxed (r) state and pdt from chlorobium tepidum in a tense (t) state. the two enzymes show low sequence identity (27.3%) but the same prototypic architecture and domain organization. both enzymes are tetramers (dimer of dimers) in crystal and so ... | 2008 | 18171624 |
| structures of open (r) and close (t) states of prephenate dehydratase (pdt)--implication of allosteric regulation by l-phenylalanine. | the enzyme prephenate dehydratase (pdt) converts prephenate to phenylpyruvate in l-phenylalanine biosynthesis. pdt is allosterically regulated by l-phe and other amino acids. we report the first crystal structures of pdt from staphylococcus aureus in a relaxed (r) state and pdt from chlorobium tepidum in a tense (t) state. the two enzymes show low sequence identity (27.3%) but the same prototypic architecture and domain organization. both enzymes are tetramers (dimer of dimers) in crystal and so ... | 2008 | 18171624 |
| pantoea agglomerans bacteremia in a 65-year-old man with acute myeloid leukemia: case report and review. | a 65-year-old man with a recent history of acute leukemia was admitted with complaints of chills and rigors. he had a long-standing hickman catheter which was removed following the isolation of a gram negative rod later identified as pantoea agglomerans. the patient recovered with antimicrobial therapy. pantoea species are rare causes of clinically relevant infections. | 2008 | 18176303 |
| effect of biocontrol agents candida sake and pantoea agglomerans on penicillium expansum growth and patulin accumulation in apples. | penicillium expansum is the major responsible of fruit pome decaying in cold storage. apples spoiled by p. expansum are expected to contain patulin, a mycotoxin which is proven to affect human health. the use of chemicals is the most common procedure to prevent rots in postharvest but legislation is becoming more and more restrictive. the use of biocontrol agents (bca) as an alternative tool is currently being proposed. the aim of this study was to evaluate the effect of two bca (candida sake cp ... | 2008 | 18191492 |
| the evolution of gene collectives: how natural selection drives chemical innovation. | dna sequencing has become central to the study of evolution. comparing the sequences of individual genes from a variety of organisms has revolutionized our understanding of how single genes evolve, but the challenge of analyzing polygenic phenotypes has complicated efforts to study how genes evolve when they are part of a group that functions collectively. we suggest that biosynthetic gene clusters from microbes are ideal candidates for the evolutionary study of gene collectives; these selfish g ... | 2008 | 18216259 |
| real-time pcr assays for quantification and differentiation of vibrio vulnificus strains in oysters and water. | vibrio vulnificus is an autochthonous estuarine bacterium and a pathogen that is frequently transmitted via raw shellfish. septicemia can occur within 24 h; however, isolation and confirmation from water and oysters require days. real-time pcr assays were developed to detect and differentiate two 16s rrna variants, types a and b, which were previously associated with environmental sources and clinical fatalities, respectively. both assays could detect 10(2) to 10(3) v. vulnificus total cells in ... | 2008 | 18245234 |
| solid-phase capture of pathogenic bacteria by using gangliosides and detection with real-time pcr. | we developed a method for concentrating pathogens from samples without enrichment. immobilized gangliosides concentrated bacteria for detection with real-time pcr. a sensitivity of approximately 4 cfu/ml (3 h) in samples without competing microflora was achieved. samples with competing microflora had a sensitivity of 40,000 cfu/ml. the variance was less than one cycle. | 2008 | 18263751 |
| conversion of beta-carotene into astaxanthin: two separate enzymes or a bifunctional hydroxylase-ketolase protein? | abstract: astaxanthin is a xanthophyll of great interest in animal nutrition and human health. the market prospect in the nutraceutics industries for this health-protective molecule is very promising. astaxanthin is synthesized by several bacteria, algae and plants from beta-carotene by the sequential action of two enzymes: a beta-carotene, 3,3'-hydroxylase that introduces an hydroxyl group at the 3 (and 3') positions of each of the two beta-ionone rings of beta-carotene, and a beta-carotene ket ... | 2008 | 18289382 |
| taxonomic structure and stability of the bacterial community in belgian sourdough ecosystems as assessed by culture and population fingerprinting. | a total of 39 traditional sourdoughs were sampled at 11 bakeries located throughout belgium which were visited twice with a 1-year interval. the taxonomic structure and stability of the bacterial communities occurring in these traditional sourdoughs were assessed using both culture-dependent and culture-independent methods. a total of 1,194 potential lactic acid bacterium (lab) isolates were tentatively grouped and identified by repetitive element sequence-based pcr, followed by sequence-based i ... | 2008 | 18310426 |
| cohesion group approach for evolutionary analysis of tyra, a protein family with wide-ranging substrate specificities. | many enzymes and other proteins are difficult subjects for bioinformatic analysis because they exhibit variant catalytic, structural, regulatory, and fusion mode features within a protein family whose sequences are not highly conserved. however, such features reflect dynamic and interesting scenarios of evolutionary importance. the value of experimental data obtained from individual organisms is instantly magnified to the extent that given features of the experimental organism can be projected u ... | 2008 | 18322033 |
| survey of bacterial diversity in chronic wounds using pyrosequencing, dgge, and full ribosome shotgun sequencing. | chronic wound pathogenic biofilms are host-pathogen environments that colonize and exist as a cohabitation of many bacterial species. these bacterial populations cooperate to promote their own survival and the chronic nature of the infection. few studies have performed extensive surveys of the bacterial populations that occur within different types of chronic wound biofilms. the use of 3 separate16s-based molecular amplifications followed by pyrosequencing, shotgun sanger sequencing, and denatur ... | 2008 | 18325110 |
| cold atmospheric plasma decontamination of the pericarps of fruit. | this investigation describes the inactivation by cold atmospheric plasmas of one pathogenic and three spoilage organisms on the pericarps of mangoes and melons. the operating voltage necessary for efficient microbial decontamination of fruit pericarps was first established using escherichia coli at a concentration of 10(7) cfu/cm2 on the surface of mango. it was found that, when the plasma was sustained slightly above its breakdown voltage of 12 kv (peak to peak), no inactivation was detected wh ... | 2008 | 18326179 |
| severe peritonitis due to pantoea agglomerans in a ccpd patient. | 2008 | 18332463 | |
| [enterobacter agglomerans b1 producing beta-galactosidase with transglycosylation activity: screening, identification, fermentation conditions, and galacto-oligosaccharides synthesis]. | galacto-oligosaccharides (gos) are promising non-digestible oligosaccharides recognized as prebiotics. commercial gos containing galactose as subunit, are synthesized from lactose using the galactosyl-transferase activity of beta-galactosidase. a strain producing beta-galactosidase with transglycosylation activity was screened from the soil. phenotypic analysis including morphology and physiology characteristics and 16s rdna sequence analysis were carried out. based on taxonomy results, the stra ... | 2008 | 18338574 |
| [isolation and identification of a novel phosphate-dissolving strain p21]. | phosphate-dissolving microorganisms can be applied for better use of insoluble phosphorus as fertilizer., a phosphate-dissolving strain p21 was isolated from soil samples in china. the isolate was identified as erwinia herbicola var. ananas, based on its 16sr dna sequence and physiological characteristics. its activity was measured in solid media as well as liquid media using different phosphate sources including tricalium phosphate, hydroxyapatite, ferric phosphate, aluminium phosphate, zinc ph ... | 2008 | 18338576 |
| bacillus spore classification via surface-enhanced raman spectroscopy and principal component analysis. | surface-enhanced raman spectroscopy (sers) can provide rapid fingerprinting of biomaterial in a nondestructive manner. the adsorption of colloidal silver to biological material suppresses native biofluorescence while providing electromagnetic surface enhancement of the normal raman signal. this work validates the applicability of qualitative ser spectroscopy for analysis of bacterial species by utilizing principal component analysis (pca) to show discrimination of biological threat simulants, ba ... | 2008 | 18339232 |
| empiric antibiotic therapy for seawater injuries: a four-seasonal analysis. | previous seawater studies found vibrio species associated with morbidity, suggesting that seawater-contaminated wounds should be treated early. the purpose of this study was to identify common seawater pathogens and antibiotic sensitivities to provide empiric antibiotic therapy until patient-specific culture results are available. | 2008 | 18349643 |
| ttsi regulates symbiotic genes in rhizobium species ngr234 by binding to tts boxes. | infection of legumes by rhizobium sp. ngr234 and subsequent development of nitrogen-fixing nodules are dependent on the coordinated actions of nod factors, proteins secreted by a type iii secretion system (t3ss) and modifications to surface polysaccharides. the production of these signal molecules is dependent on plant flavonoids which trigger a regulatory cascade controlled by the transcriptional activators nodd1, nodd2, syrm2 and ttsi. ttsi is known to control the genes responsible for t3ss fu ... | 2008 | 18363648 |
| [study of ectoparasitism of ultramicrobacteria of the genus kaistia, strains nf1 and nf3 by electron and fluorescence microscopy]. | transmission electron and fluorescence microscopy was used to study the character of the interaction of free-living ultramicrobacterial (umb) strains nf1 and nf3, affiliated with the genus kaistia, and seven species of gram-positive and gram-negative heterotrophic bacteria. strains nf1 and nf3 were found to exhibit parasitic activity against gram-positive bacillus subtilis and gram-negative acidovorax delafildii. umb cells are tightly attached to the envelopes of the victim cells and induce thei ... | 2008 | 18365722 |
| functional diversity and electron donor dependence of microbial populations capable of u(vi) reduction in radionuclide-contaminated subsurface sediments. | in order to elucidate the potential mechanisms of u(vi) reduction for the optimization of bioremediation strategies, the structure-function relationships of microbial communities were investigated in microcosms of subsurface materials cocontaminated with radionuclides and nitrate. a polyphasic approach was used to assess the functional diversity of microbial populations likely to catalyze electron flow under conditions proposed for in situ uranium bioremediation. the addition of ethanol and gluc ... | 2008 | 18378664 |
| characterization of the rnd-type multidrug efflux pump mexab-oprm of the plant pathogen pseudomonas syringae. | in gram-negative bacteria, transporters belonging to the rnd family are the transporters most relevant for resistance to antimicrobial compounds. in pseudomonas aeruginosa, a clinically important pathogen, the rnd-type pump mexab-oprm has been recognized as one of the major multidrug efflux systems. here, homologues of mexab-oprm in the plant pathogens pseudomonas syringae pv. phaseolicola 1448a, p. syringae pv. syringae b728a, and p. syringae pv. tomato dc3000 were identified, and mexab-oprm-de ... | 2008 | 18390672 |
| growth-inhibitory effects of sulfonamides at different ph: dissimilar susceptibility patterns of a soil bacterium and a test bacterium used for antibiotic assays. | the ionic speciation of sulfonamides is ph-driven and this may be crucial for their bioavailability and sorption to soil constituents, as well as for their uptake into bacterial cells. the inhibition behaviour of a bacterial test strain (pseudomonas aeruginosa; dsm 1117), which was grown in the presence of different concentrations of 8 sulfonamides at ph values from 5 to 8, could be predicted by models that take the speciation of sulfonamides in- and outside of bacterial cells into account. assu ... | 2008 | 18396316 |
| evaluation of antifungal activity of carbonate and bicarbonate salts alone or in combination with biocontrol agents in control of citrus green mold. | the aim of this research was to determine if the attacks of green mold on orange could be reduced by edible salts alone or in combination with biocontrol agent. for this purpose toxicity to pantoea digitatum and practical use of sodium carbonate (sc), sodium bicarbonate (sbc) and potassium carbonate, and potassium bicarbonate alone or in combination with antagonistic bacteria (pseudomonas fluorescens isolate pn, bacillus subtilis isolate vhn, pantoea agglomerans isolate ca) to control green mold ... | 2007 | 18396809 |
| dgge and real-time pcr analysis of lactic acid bacteria in bacterial communities of the phyllosphere of lettuce. | food associated indigenous microbial communities exert antagonistic effects on pathogens and may routinely deliver health relevant microorganisms to the gi tract. by using molecular, culture independent methods including pcr-dgge of 16s rdna-coding regions and real-time pcr (rt-pcr) as well as biolog metabolic fingerprinting, microbial communities on lettuce were analyzed in samples from fields, from supermarkets and soil. amplified 16s rrna gene sequences (57.7%) could be assigned to species pr ... | 2008 | 18398868 |
| structures of the rare-cutting restriction endonuclease noti reveal a unique metal binding fold involved in dna binding. | the structure of the rare-cutting restriction endonuclease noti, which recognizes the 8 bp target 5'-gcggccgc-3', has been solved with and without bound dna. because of its specificity (recognizing a site that occurs once per 65 kb), noti is used to generate large genomic fragments and to map dna methylation status. noti contains a unique metal binding fold, found in a variety of putative endonucleases, occupied by an iron atom coordinated within a tetrahedral cys4 motif. this domain positions n ... | 2008 | 18400177 |
| pcr-dgge assessment of the bacterial diversity of breast milk in women with lactational infectious mastitis. | infectious mastitis is a common condition during lactation and in fact, represents one of the main causes leading to a precocious weaning. the number of studies dealing with lactational mastitis is low and, up to now, the etiological diagnosis is frequently made on the basis of unspecific clinical signs. the aim of this study was to investigate the microbial diversity of breast milk in 20 women with lactational mastitis employing culture-dependent and culture-independent (pcr-dgge) approaches. | 2008 | 18423017 |
| a genomic islet mediates flagellar phase variation in escherichia coli strains carrying the flagellin-specifying locus flk. | the occurrence of unilateral flagellar phase variation was previously demonstrated in escherichia coli strains carrying the non-flic flagellin-specifying locus flk. in this study, we investigated the mechanism involved in this process. by using sequencing and sequence analysis, the flk region between the chromosomal genes yhac and rnpb was characterized in all described flk-positive e. coli strains, including the h35 strain identified in this study (the other strains used are h3, h36, h47, and h ... | 2008 | 18441064 |
| assessment of gut bacteria for a paratransgenic approach to control dermolepida albohirtum larvae. | bacteria from the hindguts of dermolepida albohirtum larvae were assessed for their potential to be used in paratransgenic strategies that target scarab pests of sugarcane. bacteria isolated in pure culture from the hindguts of d. albohirtum larvae were from the proteobacteria, firmicutes, and actinobacteria phyla and matched closely with taxa from intestinal and rhizosphere environments. however, these isolates were not the most common gut-associated bacteria identified in denaturing gradient g ... | 2008 | 18456847 |
| comparative genome analysis of lactobacillus reuteri and lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production. | lactobacillus reuteri is a heterofermentative lactic acid bacterium that naturally inhabits the gut of humans and other animals. the probiotic effects of l. reuteri have been proposed to be largely associated with the production of the broad-spectrum antimicrobial compound reuterin during anaerobic metabolism of glycerol. we determined the complete genome sequences of the reuterin-producing l. reuteri jcm 1112(t) and its closely related species lactobacillus fermentum ifo 3956. both are in the s ... | 2008 | 18487258 |
| direct comparison of the bd phoenix system with the microscan walkaway system for identification and antimicrobial susceptibility testing of enterobacteriaceae and nonfermentative gram-negative organisms. | the phoenix automated microbiology system (bd diagnostics, sparks, md) is designed for the rapid identification (id) and antimicrobial susceptibility testing (ast) of clinically significant human bacterial pathogens. we evaluated the performance of the phoenix instrument in comparison with that of the microscan walkaway system (dade behring, west sacramento, ca) in the id and ast of gram-negative clinical strains and challenge isolates of enterobacteriaceae (n = 150) and nonfermentative gram-neg ... | 2008 | 18495856 |
| healthcare-associated infections in pediatric cancer patients: results of a prospective surveillance study from university hospitals in germany and switzerland. | pediatric cancer patients face an increased risk of healthcare-associated infection (hai). to date, no prospective multicenter studies have been published on this topic. | 2008 | 18500998 |
| the exopolysaccharide of rhizobium sp. yas34 is not necessary for biofilm formation on arabidopsis thaliana and brassica napus roots but contributes to root colonization. | microbial exopolysaccharides (epss) play key roles in plant-microbe interactions, such as biofilm formation on plant roots and legume nodulation by rhizobia. here, we focused on the function of an eps produced by rhizobium sp. yas34 in the colonization and biofilm formation on non-legume plant roots (arabidopsis thaliana and brassica napus). using random transposon mutagenesis, we isolated an eps-deficient mutant of strain yas34 impaired in a glycosyltransferase gene (gta). wild type and mutant ... | 2008 | 18507672 |
| microbiological implications of periurban agriculture and water reuse in mexico city. | recycled treated or untreated wastewater represents an important health challenge in developing countries due to potential water related microbiological exposure. our aim was to assess water quality and health implications in a mexico city periurban agricultural area. | 2008 | 18509453 |
| evaluation of reuterin production in urogenital probiotic lactobacillus reuteri rc-14. | classified as a distinct species in 1980, lactobacillus reuteri strains have been used in probiotic formulations for intestinal and urogenital applications. in the former, the primary mechanism of action of l. reuteri sd2112 (atcc 55730) has been purported to be its ability to produce the antibiotic 3-hydroxypropionaldehyde (3-hpa), also known as reuterin. in the vagina, it has been postulated that probiotic lactobacillus reuteri rc-14 does not require reuterin production but mediates a restorat ... | 2008 | 18539802 |
| pantoea agglomerans: an unusual inciting agent in peritonitis. | 2008 | 18556394 | |
| antimicrobial activity of dc-159a, a new fluoroquinolone, against 1,149 recently collected clinical isolates. | the activity of dc-159a, a novel orally administered fluorinated quinolone, was evaluated by reference broth microdilution or agar dilution methods against 1,149 recently collected clinical isolates from five continents. against pathogens associated with community-acquired respiratory tract infections (ca-rtis), the mic(90)s were 0.12 microg/ml for streptococcus pneumoniae, 0.015 to 0.03 microg/ml for haemophilus influenzae, 0.03 microg/ml for moraxella catarrhalis, and 0.12 microg/ml for beta-h ... | 2008 | 18573936 |
| in vitro study of pro-inflammatory and anti-tumour properties of microvesicles from bacterial cell wall of pantoea agglomerans. | in the environment, gram-negative bacteria are capable of producing large amounts of endotoxin-containing microvesicles - spherical structures measuring 30-50 nm in diameter, emerging by fragmentation of the outer membrane of the bacterial cell wall. microvesicles are suspected of inducing inflammatory lung diseases, but possibly also of stimulating anti-tumour defence mechanisms. the present study was aimed at assessing the pro-inflammatory and anti-tumour properties of microvesicles in vitro. ... | 2008 | 18581995 |
| development of a multienzyme reactor for dopamine synthesis: i. enzymology and kinetics. | the enzymology and kinetics of tyrosine phenol lyase (tpl) from erwinia herbicola, and tyrosine decarboxylase (tdc) from streptococcus faecalis have been investigated for potential use in a coimmobilized multienzyme biocatalytic system for the production of dopamine. in this multienzyme biotransformation using whole cells optimized for each of the respective enzymes, tpl catalyzes the production of 3,4-dihydroxyphenyl-l-alanine (l-dopa) from catechol, pyruvate, and ammonium, and this is subseque ... | 1992 | 18601010 |
| development of a multienzyme reactor for dopamine synthesis: ii. reactor engineering and simulation. | aspects of reaction engineering associated with multienzyme reactions have been studied in a system where dopamine is produced from catechol, pyruvate and ammonium by sequential enzymatic reactions catalyzed by tyrosine phenol lyase (tpl) and tyrosine decarboxylase (tdc). microbial cells containing tpl activity (erwinia herbicola) and tdc activity (streptococcus faecalis) were coimmobilized in glutaraldehyde cross-linked porcine gelatin beads with a mean diameter of 2.8 mm for use in the reactio ... | 1992 | 18601129 |
| continuous culture system for production of biopolymer levan using erwinia herbicola. | the optimal production of the fructan biopolymer levan by the bacterium erwinia herbicola was investigated, including variations in nitrogen, carbon and phosphorous sources, ph, incubation time, culture yields up to 19% by weight produced based on conversion of sucrose as the carbon source when grown in a continuous culture system and processed by tangential flow filtration. product identity was confirmed with gas chromatography (gc) and (13)c nuclear magnetic resonance (nmr). gel permeation chr ... | 1991 | 18604815 |
| quorum-sensing system affects gall development incited by pantoea agglomerans pv. gypsophilae. | the quorum-sensing (qs) regulatory system of the gall-forming pantoea agglomerans pv. gypsophilae was identified. mass spectral analysis, together with signal-specific biosensors, demonstrated that p. agglomerans pv. gypsophilae produced n-butanoyl-l-homoserine lactone (c4-hsl) as a major and n-hexanoyl-l-homoserine lactone (c6-hsl) as a minor qs signal. homologs of luxi and luxr regulatory genes, pagi and pagr, were characterized in strain p. agglomerans pv. gypsophilae pag824-1 and shown to be ... | 2008 | 18616406 |
| persistence of salmonella senftenberg in poultry production environments and investigation of its resistance to desiccation. | most salmonella serovars, including salmonella enterica subsp. enterica serovar senftenberg (s. senftenberg), are tolerant to desiccation and able to colonize and persist in feed mills. in addition, they may survive cleaning and disinfection procedures used on poultry farms. the present study was conducted to investigate the survival of s. senftenberg in broiler parent stock farms and broiler farms. the isolates from one of the parent stock farms investigated only differed by a single band in fl ... | 2008 | 18622860 |
| characterization of free and alginate-polylysine-alginate microencapsulated erwinia herbicola for the conversion of ammonia, pyruvate, and phenol into l-tyrosine. | the whole cell tyrosine phenol-lyase (tpl, e.c. 4.1.99.2) activity of erwinia herbicola (atcc 21434) was microen-capsulated. we studied the use of this for the conversion of ammonia and pyruvate along with phenol or catechol, respectively, into l-tyrosine or dihydroxyphenyl-l-alanine (l-dopa). the reactions are relevant to the development of new methods for the production of l-tyrosine and l-dopa. the growth of e. herbicola at temperatures from 22 degrees c to 32 degrees c is stable, since at th ... | 1995 | 18623540 |
| native bacterial endophytes promote host growth in a species-specific manner; phytohormone manipulations do not result in common growth responses. | all plants in nature harbor a diverse community of endophytic bacteria which can positively affect host plant growth. changes in plant growth frequently reflect alterations in phytohormone homoeostasis by plant-growth-promoting (pgp) rhizobacteria which can decrease ethylene (et) levels enzymatically by 1-aminocyclopropane-1-carboxylate (acc) deaminase or produce indole acetic acid (iaa). whether these common pgp mechanisms work similarly for different plant species has not been rigorously teste ... | 2008 | 18628963 |
| bacteremia in equine neonatal diarrhea: a retrospective study (1990-2007). | bacteremia in sick foals is associated with survival, but the association of bacteremia and diarrhea is not reported. | 2008 | 18638014 |
| arsenite oxidation by alcaligenes sp. strain rs-19 isolated from arsenic-contaminated mines in the republic of korea. | arsenite [as(iii)]-oxidizing bacteria play important roles in reducing arsenic [as] toxicity and mobility in as-contaminated areas. as-resistant bacteria were isolated from the soils of two abandoned mines in the republic of korea. the isolated bacteria showed relatively high resistances to as(iii) up to 26 mm. the pcr-based 16s rrna analysis revealed that the isolated as-resistant bacteria were close relatives to serratia marcescensa, pseudomonas putida, pantoea agglomerans, and alcaligenes sp. ... | 2009 | 18642094 |
| gatekeeping versus promiscuity in the early stages of the andrimid biosynthetic assembly line. | the antibiotic andrimid, a nanomolar inhibitor of bacterial acetyl coenzyme a carboxylase, is generated on an unusual polyketide/nonribosomal peptide enzyme assembly line in that all thiolation (t) domains/small-molecule building stations are on separate proteins. in addition, a transglutaminase homologue is used to condense andrimid building blocks together on the andrimid assembly line. the first two modules of the andrimid assembly line yields an octatrienoyl-beta-phe-thioester tethered to th ... | 2008 | 18652473 |
| anti-adherence activity and antimicrobial durability of anti-infective-coated catheters against multidrug-resistant bacteria. | to investigate the anti-adherence and antimicrobial durability of anti-infective catheters against multidrug-resistant (mdr) staphylococcus aureus (resistant to vancomycin, rifampicin and methicillin) and mdr gram-negative bacteria (stenotrophomonas maltophilia, acinetobacter baumannii/calcoaceticus and enterobacter agglomerans) that are often associated with catheter-related bloodstream infections (crbsis). | 2008 | 18653489 |
| phytopathogen type iii effector weaponry and their plant targets. | phytopathogenic bacteria suppress plant innate immunity and promote pathogenesis by injecting proteins called type iii effectors into plant cells using a type iii protein secretion system. these type iii effectors use at least three strategies to alter host responses. one strategy is to alter host protein turnover, either by direct cleavage or by modulating ubiquitination and targeting the 26s proteasome. another strategy involves alteration of rna metabolism by transcriptional activation or adp ... | 2008 | 18657470 |
| [intestinal enterobacteria of the hibernating apis mellifera mellifera l. bees]. | dynamics of enterobacteria of normal intestinal microflora was studied in apis mellifera mellifera l. bees hibernating under snow in the western urals. the cell numbers (n) of the predominant species klebsiella oxytoca increased from 10-10(6) cfu/bee in november 2004 to 10(4)-10(7) cfu/bee in march 2005; its frequency of occurrence (p) increased from 92 to 100%. increase of providencia rettgeri (11.2004: n up to 10(6), p 25%; 03.2005: n 10(2)-10(6), p 80%) was accompanied by the substitution of ... | 2008 | 18683661 |
| yeast cell factories for fine chemical and api production. | abstract: this review gives an overview of different yeast strains and enzyme classes involved in yeast whole-cell biotransformations. a focus was put on the synthesis of compounds for fine chemical and api (= active pharmaceutical ingredient) production employing single or only few-step enzymatic reactions. accounting for recent success stories in metabolic engineering, the construction and use of synthetic pathways was also highlighted. examples from academia and industry and advances in the f ... | 2008 | 18684335 |
| use of antibacterial consumer products containing quaternary ammonium compounds and drug resistance in the community. | 2008 | 18694904 | |
| characterization of the tautomycin biosynthetic gene cluster from streptomyces spiroverticillatus unveiling new insights into dialkylmaleic anhydride and polyketide biosynthesis. | tautomycin (ttm) is a highly potent and specific protein phosphatase inhibitor isolated from streptomyces spiroverticillatus. the biological activity of ttm makes it an important lead for drug discovery, whereas its spiroketal-containing polyketide chain and rare dialkylmaleic anhydride moiety draw attention to novel biosynthetic chemistries responsible for its production. to elucidate the biosynthetic machinery associated with these novel molecular features, the ttm biosynthetic gene cluster fr ... | 2008 | 18708355 |
| diversity of endophytic bacteria from eucalyptus species seeds and colonization of seedlings by pantoea agglomerans. | the diversity and beneficial characteristics of endophytic microorganisms have been studied in several host plants. however, information regarding naturally occurring seed-associated endophytes and vertical transmission among different life-history stages of hosts is limited. endophytic bacteria were isolated from seeds and seedlings of 10 eucalyptus species and two hybrids. the results showed that endophytic bacteria, such as bacillus, enterococcus, paenibacillus and methylobacterium, are verti ... | 2008 | 18710397 |
| insights into the catalytic mechanism of tyrosine phenol-lyase from x-ray structures of quinonoid intermediates. | amino acid transformations catalyzed by a number of pyridoxal 5'-phosphate (plp)-dependent enzymes involve abstraction of the calpha proton from an external aldimine formed between a substrate and the cofactor leading to the formation of a quinonoid intermediate. despite the key role played by the quinonoid intermediates in the catalysis by plp-dependent enzymes, limited accurate information is available about their structures. we trapped the quinonoid intermediates of citrobacter freundii tyros ... | 2008 | 18715865 |
| aromatic amino acid-dependent expression of indole-3-pyruvate decarboxylase is regulated by tyrr in enterobacter cloacae uw5. | the plant growth-promoting rhizobacterium enterobacter cloacae uw5 synthesizes the plant growth hormone indole-3-acetic acid (iaa) via the indole-3-pyruvate pathway utilizing the enzyme indole-3-pyruvate decarboxylase that is encoded by ipdc. in this bacterium, ipdc expression and iaa production occur in stationary phase and are induced by an exogenous source of tryptophan, conditions that are present in the rhizosphere. the aim of this study was to identify the regulatory protein that controls ... | 2008 | 18757531 |
| identification and functional characterization of nifa variants that are independent of glnb activation in the photosynthetic bacterium rhodospirillum rubrum. | the activity of nifa, the transcriptional activator of the nitrogen fixation (nif) gene, is tightly regulated in response to ammonium and oxygen. however, the mechanisms for the regulation of nifa activity are quite different among various nitrogen-fixing bacteria. unlike the well-studied nifl-nifa regulatory systems in klebsiella pneumoniae and azotobacter vinelandii, in rhodospirillum rubrum nifa is activated by a direct protein-protein interaction with the uridylylated form of glnb, which in ... | 2008 | 18757802 |
| diversity of endophytic enterobacteria associated with different host plants. | fifty-three endophytic enterobacteria isolates from citrus, cocoa, eucalyptus, soybean, and sugar cane were evaluated for susceptibility to the antibiotics ampicillin and kanamycin, and cellulase production. susceptibility was found on both tested antibiotics. however, in the case of ampicillin susceptibility changed according to the host plant, while all isolates were susceptible to kanamycin. cellulase production also changed according to host plants. the diversity of these isolates was estima ... | 2008 | 18758726 |
| endophytic population of pantoea agglomerans in citrus plants and development of a cloning vector for endophytes. | harmless bacteria inhabiting inner plant tissues are termed endophytes. population fluctuations in the endophytic bacterium pantoea agglomerans associated with two species of field cultured citrus plants were monitored over a two-year period. the results demonstrated that populations of p. agglomerans fluctuated in citrus reticulata but not c. sinensis. a cryptic plasmid ppa3.0 (2.9 kb) was identified in 35 out of 44 endophytic isolates of p. agglomerans and was subsequently sequenced. the origi ... | 2008 | 18759238 |
| andrimid producers encode an acetyl-coa carboxyltransferase subunit resistant to the action of the antibiotic. | andrimid is a hybrid nonribosomal peptide-polyketide antibiotic that blocks the carboxyl-transfer reaction of bacterial acetyl-coa carboxylase (acc) and thereby inhibits fatty acid biosynthesis with submicromolar potency. the andrimid biosynthetic gene cluster from pantoea agglomerans encodes an admt gene with homology to the acetyl-coa carboxyltransferase (ct) beta-subunit gene accd. escherichia coli cells overexpressing admt showed resistance to andrimid. co-overproduction of admt with e. coli ... | 2008 | 18768797 |
| case report: subacute synovitis of the knee after a rose thorn injury: unusual clinical picture. | synovitis secondary to penetrating plant thorn injuries is not frequently reported. historically, it is considered aseptic and treated with removal of the intraarticular foreign body and affected synovial lining. we report a 57-year-old healthy man who was admitted 2 weeks after being injured by a rose (rosacea) thorn with subacute and mild synovitis with effusion of his right knee. no intraarticular foreign body was retained. pantoea agglomerans was identified in the synovial fluid. contrary to ... | 2008 | 18773251 |
| in vitro adsorption revealing an apparent strong interaction between endophyte pantoea agglomerans ys19 and host rice. | pantoea (formerly enterobacter) agglomerans ys19 is a dominant diazotrophic endophyte isolated from rice (oryza sativa cv. yuefu) grown in a temperate-climate region in west beijing, china. in vitro adsorption and invasion of ys19 on host plant root were studied in this research. adsorption of ys19 on rice seedling roots closely resembled the langmuir adsorption and showed a higher adsorption quantity than the control strains paenibacillus polymyxa wy110 (a rhizospheric bacterium from the same r ... | 2008 | 18781359 |
| staphylococcus epidermidis: a differential trait of the fecal microbiota of breast-fed infants. | breast milk is an important source of staphylococci and other bacterial groups to the infant gut. the objective of this work was to analyse the bacterial diversity in feces of breast-fed infants and to compare it with that of formula-fed ones. a total of 23 women and their respective infants (16 breast-fed and 7 formula-fed) participated in the study. the 16 women and their infants provided a sample of breast milk and feces, respectively, at days 7, 14, and 35. the samples were plated onto diffe ... | 2008 | 18783615 |
| divergence among genes encoding the elongation factor tu of yersinia species. | elongation factor tu (ef-tu), encoded by tuf genes, carries aminoacyl-trna to the ribosome during protein synthesis. duplicated tuf genes (tufa and tufb), which are commonly found in enterobacterial species, usually coevolve via gene conversion and are very similar to one another. however, sequence analysis of tuf genes in our laboratory has revealed highly divergent copies in 72 strains spanning the genus yersinia (representing 12 yersinia species). the levels of intragenomic divergence between ... | 2008 | 18790860 |
| identification of bacterial and fungal components in tobacco and tobacco smoke. | abstract: the microbiological composition of tobacco products was studied using culture and chemical analysis (of tobacco leaves) or chemical analysis only (tobacco and tobacco smoke). the chemical analyses utilized gas chromatography-tandem mass spectrometry for determining 3-hydroxy fatty acids, muramic acid, and ergosterol as markers of respectively lipopolysaccharide (lps), peptidoglycan, and fungal biomass. mesophilic bacteria dominated in both fresh and cured tobacco leaves; a range of add ... | 2008 | 18822161 |
| pantoea agglomerans bloodstream infection in preterm neonates. | objective: to report an uncommon incidence of sporadic bloodstream infection (bsi) caused by pantoea agglomerans in preterm neonates. case presentation and intervention: fives cases of nosocomial bsi with p. agglomerans in preterm neonates (weight <or=1,500 g; age 8-17 days; gestational age 26-30 weeks) are presented. all cases were late onset neonatal sepsis (>7 days of age). lethargy, skin mottling and bradycardia were often present. although there was no evidence of pneumonia, desaturation wa ... | 2008 | 18836282 |
| altered oligomerization properties of n316 mutants of escherichia coli tyrr. | the transcriptional regulator tyrr is known to undergo a dimer-to-hexamer conformational change in response to aromatic amino acids, through which it controls gene expression. in this study, we identified n316d as the second-site suppressor of escherichia coli tyrr(e274q), a mutant protein deficient in hexamer formation. n316 variants exhibited altered in vivo regulatory properties, and the most drastic changes were observed for tyrr(n316d) and tyrr(n316r) mutants. gel filtration analyses reveal ... | 2008 | 18931124 |
| temperature and pomaceous flower age related to colonization by erwinia amylovora and antagonists. | abstract fire blight of apple and pear is initiated by epiphytic populations of erwinia amylovora on flower stigmas. predicting this disease and managing it with microbial antagonists depends on an understanding of bacterial colonization on stigmas. detached 'manchurian' crab apple flowers were inoculated with e. amylovora and subjected to a range of constant temperatures or various fluctuating temperature regimes. results may have application to disease risk assessment systems such as the couga ... | 2004 | 18943112 |
| antibiosis and acidification by pantoea agglomerans strain e325 may contribute to suppression of erwinia amylovora. | pantoea agglomerans strain e325, a commercially available antagonist for fire blight of apple and pear, was originally selected through screening based on suppression of erwinia amylovora on flower stigmas, but specific mechanisms of antagonism were unknown. bacterial modification of ph was evaluated as a possible mechanism by analyzing stigma exudates extracted from 'gala' apple stigmas. the ph values for field samples were only slightly lower than controls, but indicated a range (ph 5 to 6) co ... | 2008 | 18943460 |
| comparative dynamics of adherent and nonadherent bacterial populations on maize leaves. | abstract the dynamics of the adherent and nonadherent populations of three bacterial species on maize leaves were examined to identify the extent to which bacteria adhere to leaves and the importance of this adhesion to leaf colonization. pantoea agglomerans strain brt98, clavibacter michiganensis subsp. nebraskensis strain gh2390, and pseudomonas syringae pv. syringae strain hs191r all rapidly adhered to maize leaves following inoculation, but differed in the percentage of cells that adhered to ... | 2002 | 18944027 |
| antibiosis contributes to biological control of fire blight by pantoea agglomerans strain eh252 in orchards. | abstract fire blight, caused by erwinia amylovora, is the most serious bacterial disease of pear and apple trees. biological control with strains of pantoea agglomerans (syn. erwinia herbicola) may provide an effective disease management strategy for fire blight. most strains of p. agglomerans evaluated for suppression of fire blight produce compounds that inhibit the growth of e. amylovora in culture. the role of these inhibitory compounds in fire blight suppression in orchard environments has ... | 2002 | 18944246 |
| assessment of environmental factors influencing growth and spread of pantoea agglomerans on and among blossoms of pear and apple. | abstract we evaluated effects of both physical and biological components of the environment on growth of pantoea agglomerans on inoculated pear and apple blossoms and on spread of the bacterium to blossoms on non-inoculated trees. the center three rows of 0.35- to 0.5-ha blocks of four pear cultivars and four apple cultivars were sprayed with a suspension of streptomycin-resistant p. agglomerans strain c9-1s (c9-1s) at 20 to 60% and 60 to 90% bloom. cultivars were chosen to create a sequence of ... | 2000 | 18944433 |
| anatomy of cranberry stem gall and localization of bacteria in galls. | abstract cranberry stem gall is characterized by tumors that girdle stems, thereby killing all distal leaves, flowers, and fruit. bacteria that produce high levels of the plant growth hormone indole-3-acetic acid (iaa) are associated with and believed to cause cranberry stem gall. the anatomy of naturally occurring galls on woody cranberry plants and galls caused by inoculation of micropropagated cranberry plants with pantoea agglomerans strain 4/99 was consistent with elevated levels of iaa in ... | 2004 | 18944452 |
| an extracellular protease of pseudomonas fluorescens inactivates antibiotics of pantoea agglomerans. | abstract pseudomonas fluorescens a506 and pantoea agglomerans strains eh252 and c9-1 are biological control agents that suppress fire blight, an important disease of pear and apple caused by the bacterium erwinia amylovora. pseudomonas fluorescens strain a506 suppresses disease largely through competitive exclusion of e. amylovora on surfaces of blossoms, the primary infection court, whereas pantoea agglomerans strains eh252 and c9-1 produce antibiotics that are toxic to e. amylovora. in this st ... | 2004 | 18944458 |
| a rapid bioassay for screening rhizosphere microorganisms for their ability to induce systemic resistance. | abstract we developed a rapid and miniaturized bioassay for screening large numbers of rhizosphere microorganisms for their ability to induce systemic resistance to bacterial leaf spot of radish caused by xanthomonas campestris pv. armoraciae. in this bioassay, pantoea agglomerans strain e278ar controlled symptoms of disease as effectively as 2,6-dichloroisonicotinic acid when applied to the roots of seedlings produced in growth pouches in a soilless system. e278ar essentially did not migrate fr ... | 2000 | 18944580 |
| biological control of pseudomonas syringae pv. syringae, the causal agent of basal kernel blight of barley, by antagonistic pantoea agglomerans. | abstract strains of pantoea agglomerans (synanamorph erwinia herbicola) suppressed the development of basal kernel blight of barley, caused by pseudomonas syringae pv. syringae, when applied to heads prior to the pseudomonas syringae pv. syringae infection window at the soft dough stage of kernel development. field experiments in 1994 and 1995 revealed 45 to 74% kernel blight disease reduction, whereas glasshouse studies resulted in 50 to 100% disease control depending on the isolate used and ba ... | 2000 | 18944586 |
| effect of nectar on microbial antagonists evaluated for use in control of fire blight of pome fruits. | abstract under warm, dry conditions, erwinia amylovora can become established in relatively high populations on apple (malus domestica) or pear (pyrus communis) flower stigmas, and subsequent wet conditions facilitate its movement to the flower hypanthium where infection generally is initiated through the nectarthodes. research on biological control of fire blight has focused mainly on the flower stigma, and knowledge is lacking regarding the effect of nectar on microbial antagonists in the flow ... | 1999 | 18944801 |
| occurrence of indole-3-acetic acid-producing bacteria on pear trees and their association with fruit russet. | abstract a relatively high percentage of epiphytic bacteria on pear leaf and fruit surfaces had the ability to produce indole-3-acetic acid (iaa) in culture media supplemented with tryptophan. while over 50% of the strains produced at least small amounts of iaa in culture, about 25% of the strains exhibited high iaa production as evidenced by both colorimetric and high-performance liquid chromatography analysis of culture supernatants. a majority of the strains that produced high amounts of iaa ... | 1998 | 18944847 |
| compost and compost water extract-induced systemic acquired resistance in cucumber and arabidopsis. | abstract a biocontrol agent-fortified compost mix, suppressive to several diseases caused by soilborne plant pathogens, induced systemic acquired resistance (sar) in cucumber against anthracnose caused by colletotrichum orbiculare and in arabidopsis against bacterial speck caused by pseudomonas syringae pv. maculicola kd4326. a peat mix conducive to soilborne diseases did not induce sar. the population size of p. syringae pv. maculicola kd4326 was significantly lower in leaves of arabidopsis pla ... | 1998 | 18944926 |
| crab apple blossoms as a model for research on biological control of fire blight. | abstract nonseasonal availability of pomaceous flowers could improve laboratory detection and prefield testing of biocontrol agents for fire blight of pear and apple. crab apple was selected as a model because of its high flower productivity on 1-year-old wood, high susceptibility to fire blight, and availability from nurseries. cultivars manchurian and snowdrift were manipulated to bloom once by transferring dormant nursery trees from a cold room to a greenhouse and a second time by defoliating ... | 1997 | 18945005 |
| evaluation of sample preparation methods for the isolation of salmonella from alfalfa and mung bean seeds with the bacteriological analytical manual's salmonella culture method. | five pre-enrichment methods were evaluated for effectiveness with the u.s. food and drug administration's bacteriological analytical manual salmonella culture method in recovering s. stanley, s. poona, and s. muenchen from artificially contaminated alfalfa seeds, and s. saintpaul, s. anatum, and s. infantis from artificially contaminated mung bean seeds. the methods included: (1) soak.--test portions were inoculated into pre-enrichment media; (2) rinse.--test portions were rinsed with pre-enrich ... | 2008 | 18980122 |
| reduced diversity and increased virulence-gene carriage in intestinal enterobacteria of coeliac children. | coeliac disease is an immune-mediated enteropathology triggered by the ingestion of cereal gluten proteins. this disorder is associated with imbalances in the composition of the gut microbiota that could be involved in its pathogenesis. the aim of the present study was to determine whether intestinal enterobacteriaceae populations of active and non-active coeliac patients and healthy children differ in diversity and virulence-gene carriage, so as to establish a possible link between the pathogen ... | 2008 | 18983674 |
| xylella fastidiosa afimbrial adhesins mediate cell transmission to plants by leafhopper vectors. | the interactions between the economically important plant-pathogenic bacterium xylella fastidiosa and its leafhopper vectors are poorly characterized. we used different approaches to determine how x. fastidiosa cells interact with the cuticular surface of the foreguts of vectors. we demonstrate that x. fastidiosa binds to different polysaccharides with various affinities and that these interactions are mediated by cell surface carbohydrate-binding proteins. in addition, competition assays showed ... | 2009 | 19011051 |
| xylella fastidiosa afimbrial adhesins mediate cell transmission to plants by leafhopper vectors. | the interactions between the economically important plant-pathogenic bacterium xylella fastidiosa and its leafhopper vectors are poorly characterized. we used different approaches to determine how x. fastidiosa cells interact with the cuticular surface of the foreguts of vectors. we demonstrate that x. fastidiosa binds to different polysaccharides with various affinities and that these interactions are mediated by cell surface carbohydrate-binding proteins. in addition, competition assays showed ... | 2009 | 19011051 |
| geographic, seasonal, and precipitation chemistry influence on the abundance and activity of biological ice nucleators in rain and snow. | biological ice nucleators (in) function as catalysts for freezing at relatively warm temperatures (warmer than -10 degrees c). we examined the concentration (per volume of liquid) and nature of in in precipitation collected from montana and louisiana, the alps and pyrenees (france), ross island (antarctica), and yukon (canada). the temperature of detectable ice-nucleating activity for more than half of the samples was > or = -5 degrees c based on immersion freezing testing. digestion of the samp ... | 2008 | 19028877 |
| evaluation of an immunochromatographic assay kit for rapid identification of mycobacterium tuberculosis complex in clinical isolates. | we evaluated a new immunochromatographic assay (ica) using mouse monoclonal anti-mpt64 antibody for rapid discrimination between mycobacterium tuberculosis and nontuberculous mycobacteria in clinical isolates. a study with mycobacteria and other organisms showed excellent sensitivity (approximately equal 99%) and specificity (100%) and an appropriate detection limit (10(5) cfu/ml) when tested with m. tuberculosis h37rv. this ica can simplify the identification of m. tuberculosis in clinical labo ... | 2009 | 19052177 |
| evaluation of an immunochromatographic assay kit for rapid identification of mycobacterium tuberculosis complex in clinical isolates. | we evaluated a new immunochromatographic assay (ica) using mouse monoclonal anti-mpt64 antibody for rapid discrimination between mycobacterium tuberculosis and nontuberculous mycobacteria in clinical isolates. a study with mycobacteria and other organisms showed excellent sensitivity (approximately equal 99%) and specificity (100%) and an appropriate detection limit (10(5) cfu/ml) when tested with m. tuberculosis h37rv. this ica can simplify the identification of m. tuberculosis in clinical labo ... | 2009 | 19052177 |
| phylogeny and identification of pantoea species and typing of pantoea agglomerans strains by multilocus gene sequencing. | pantoea agglomerans and other pantoea species cause infections in humans and are also pathogenic to plants, but the diversity of pantoea strains and their possible association with hosts and disease remain poorly known, and identification of pantoea species is difficult. we characterized 36 pantoea strains, including 28 strains of diverse origins initially identified as p. agglomerans, by multilocus gene sequencing based on six protein-coding genes, by biochemical tests, and by antimicrobial sus ... | 2009 | 19052179 |
| characterization of the mineral phosphate-solubilizing activity of pantoea agglomerans mmb051 isolated from an iron-rich soil in southeastern venezuela (bolívar state). | the mineral phosphate-solubilizing (mps) activity of a pantoea agglomerans strain, namely mmb051, isolated from an iron-rich, acidic soil near ciudad piar (bolívar state, venezuela), was characterized on a chemically defined medium (nbrip). various insoluble inorganic phosphates, including tri-calcium phosphate [ca(3)(po(4))(2)], iron phosphate (fepo(4)), aluminum phosphate (alpo(4)), and rock phosphate (rp) were tested as sole sources of p for bacterial growth. solubilization of ca(3)(po(4))(2) ... | 2009 | 19067045 |