Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| gram-stain-based antimicrobial selection reduces cost and overuse compared with japanese guidelines. | the gram stain has been used as an essential tool for antimicrobial stewardship in our hospital since the 1970s. the objective of this study was to clarify the difference in the targeted therapies selected based on the gram stain and simulated empirical therapies based on the antimicrobial guidelines used in japan. | 2015 | 26503359 |
| a structure-based classification of class a β-lactamases, a broadly diverse family of enzymes. | for medical biologists, sequencing has become a commonplace technique to support diagnosis. rapid changes in this field have led to the generation of large amounts of data, which are not always correctly listed in databases. this is particularly true for data concerning class a β-lactamases, a group of key antibiotic resistance enzymes produced by bacteria. many genomes have been reported to contain putative β-lactamase genes, which can be compared with representative types. we analyzed several ... | 2016 | 26511485 |
| a structure-based classification of class a β-lactamases, a broadly diverse family of enzymes. | for medical biologists, sequencing has become a commonplace technique to support diagnosis. rapid changes in this field have led to the generation of large amounts of data, which are not always correctly listed in databases. this is particularly true for data concerning class a β-lactamases, a group of key antibiotic resistance enzymes produced by bacteria. many genomes have been reported to contain putative β-lactamase genes, which can be compared with representative types. we analyzed several ... | 2016 | 26511485 |
| characterization of genetic structures of the qepa3 gene in clinical isolates of enterobacteriaceae. | qepa is one of the genes that confer quinolone resistance in bacteria. the aim of this study was to analyze the genetic structures of plasmids that carry a qepa3, a recently discovered allele of qepa in enterobacteriaceae clinical isolates. 656 non-redundant enterobacteriaceae clinical isolates were screened for the qepa3 gene and five isolates were identified to carry the gene. plasmids were isolated from these isolates and were found to increase antibiotic resistance once the plasmids were tra ... | 2015 | 26528280 |
| multicenter evaluation of the bruker maldi biotyper ca system for the identification of clinical aerobic gram-negative bacterial isolates. | the prompt and accurate identification of bacterial pathogens is fundamental to patient health and outcome. recent advances in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) have revolutionized bacterial identification in the clinical laboratory, but uniform incorporation of this technology in the u.s. market has been delayed by a lack of fda-cleared systems. in this study, we conducted a multicenter evaluation of the maldi biotyper ca (mbt-ca) system ... | 2015 | 26529504 |
| neuron-astrocyte interactions in neurodegenerative diseases: role of neuroinflammation. | selective neuron loss in discrete brain regions is a hallmark of various neurodegenerative disorders, although the mechanisms responsible for this regional vulnerability of neurons remain largely unknown. earlier studies attributed neuron dysfunction and eventual loss during neurodegenerative diseases as exclusively cell autonomous. although cell-intrinsic factors are one critical aspect in dictating neuron death, recent evidence also supports the involvement of other central nervous system cell ... | 2015 | 26543505 |
| management of meningitis caused by multi drug-resistant acinetobacter baumannii: clinical, microbiological and pharmacokinetic results in a patient treated with colistin methanesulfonate. | this paper reports on a 71- year-old caucasian male who underwent neurosurgery for an oligodendroglioma, followed by a cranial-sinus fistula and cerebrospinal fluid rhinorrhea. the clinical course was complicated due to an extensively drug-resistant acinetobacter baumannii meningitis. the patient was treated with colistin methanesulfonate, intrathecal for 24 days and intravenous for 46 days. in addition, the patient received meropenem and teicoplanin to treat a urinary tract infection and a bact ... | 2015 | 26543524 |
| development of a rapid and simplified protocol for direct bacterial identification from positive blood cultures by using matrix assisted laser desorption ionization time-of- flight mass spectrometry. | bloodstream infections represent serious conditions carrying a high mortality and morbidity rate. rapid identification of microorganisms and prompt institution of adequate antimicrobial therapy is of utmost importance for a successful outcome. aiming at the development of a rapid, simplified and efficient protocol, we developed and compared two in-house preparatory methods for the direct identification of bacteria from positive blood culture flasks (bd bactec fx system) by using matrix-assisted ... | 2015 | 26546000 |
| does percutaneous dilatational tracheostomy increase the incidence of sternal wound infection - a single center retrospective of 4100 cases. | the impact of percutaneous dilatational tracheostomy (pdt) on the development of post-median sternotomy wound infection (swi) and mediastinitis is still controversial. we aimed to investigate the frequency of cross-infection and incidence of swi after pdt. | 2015 | 26546171 |
| [septic shock following platelet transfusion contaminated with citrobacter koseri in a child with postchemotherapy febrile neutropenia]. | the bacterial transfusion risk is currently the greatest infectious risk of blood transfusion. we report the case of a child with postchemotherapy febrile neutropenia who presented septic shock following platelet transfusion contaminated with citrobacter koseri. the life-threatening development could have been avoided by strict compliance with good clinical practice. the stability of mortality rates due to adverse effects of bacterial proliferation during platelet transfusions in france since 19 ... | 2016 | 26552624 |
| neonatal brain abscess: an atypical presentation. | brain abscesses occur as an uncommon complication of bacterial meningitis in the neonatal period. a 34 weeks preterm at-risk neonate presented with abnormal breathing pattern and inability to maintain the oxygen saturation in room air. magnetic resonance imaging (mri) study revealed intra-parenchymal brain abscesses in the left basal ganglion and bilateral fronto-parietal regions. intravenous piperacillin - tazobactam was commenced and continued for 6 weeks in neonatal intensive care unit. no su ... | 2015 | 26557178 |
| bacteriological profile of surgical site infections and their antibiogram: a study from resource constrained rural setting of uttarakhand state, india. | surgical site infections (ssi) constitute a major public health problem worldwide and are the second most frequently reported nosocomial infections. they are responsible for increasing the treatment cost, length of hospital stay and significant morbidity and mortality. | 2015 | 26557520 |
| evaluation of an image analysis device (apas) for screening urine cultures. | while advancements have been made in some areas of pathology with diagnostic materials being screened using image analysis technologies, the reporting of cultures from agar plates remains a manual process. we compared the results for 2,163 urine cultures read by a reference panel of microbiologists, by the routine laboratory process, and by an automated plate reading system, apas (lbt innovations ltd., south australia). apas detected colonies with a sensitivity of 99.1% and a specificity of 99.3 ... | 2016 | 26582838 |
| predictors of bacteraemia in patients with suspected community-acquired pneumonia. | the diagnostic yield of blood cultures is limited in patients with community-acquired pneumonia (cap). yet, positive blood culture results provide important information for antibiotic treatment and for monitoring epidemiologic trends. we investigated the potential of clinical predictors to improve the cost-benefit ratio of obtaining blood cultures. | 2015 | 26599636 |
| laboratory-based nationwide surveillance of antimicrobial resistance in ghana. | global efforts are underway to combat antimicrobial resistance (amr). a key target in this intervention is surveillance for local and national action. data on amr in ghana are limited, and monitoring of amr is nonexistent. we sought to generate baseline data on amr, and to assess the readiness of ghana in laboratory-based surveillance. biomedical scientists in laboratories across ghana with capacity to perform bacteriological culture were selected and trained. in-house standard operating protoco ... | 2015 | 26604806 |
| detection of carbapenemase production in a collection of enterobacteriaceae with characterized resistance mechanisms from clinical and environmental origins by use of both carba np and blue-carba tests. | rapid-screening methods to confirm the presence of resistance mechanisms in multidrug-resistant bacteria are currently recommended. carba np and blue-carba tests were evaluated in carbapenemase-producing enterobacteriaceae from hospital (n = 102) and environmental (n = 57) origins for detecting the different molecular classes among them. both methods showed to be fast and cost-effective, with high sensitivity (98% to 100%) and specificity (100%), and may be easily introduced in the routine labor ... | 2016 | 26607979 |
| individuality, phenotypic differentiation, dormancy and 'persistence' in culturable bacterial systems: commonalities shared by environmental, laboratory, and clinical microbiology. | for bacteria, replication mainly involves growth by binary fission. however, in a very great many natural environments there are examples of phenotypically dormant, non-growing cells that do not replicate immediately and that are phenotypically 'nonculturable' on media that normally admit their growth. they thereby evade detection by conventional culture-based methods. such dormant cells may also be observed in laboratory cultures and in clinical microbiology. they are usually more tolerant to s ... | 2015 | 26629334 |
| associations between single nucleotide polymorphisms of high mobility group box 1 protein and clinical outcomes in korean sepsis patients. | high mobility group box 1 (hmgb1) plays a central role in the pathogenesis of sepsis and multiple organ dysfunction syndromes. we investigated the associations of a single nucleotide polymorphism (snp; rs1045411) in hmgb1 with various clinical parameters, severity, and prognosis in patients with sepsis, severe sepsis, or septic shock. | 2015 | 26632390 |
| associations between single nucleotide polymorphisms of high mobility group box 1 protein and clinical outcomes in korean sepsis patients. | high mobility group box 1 (hmgb1) plays a central role in the pathogenesis of sepsis and multiple organ dysfunction syndromes. we investigated the associations of a single nucleotide polymorphism (snp; rs1045411) in hmgb1 with various clinical parameters, severity, and prognosis in patients with sepsis, severe sepsis, or septic shock. | 2015 | 26632390 |
| community-acquired pneumonia and empyema caused by citrobacter koseri in an immunocompetent patient. | citrobacter species, belonging to the family enterobacteriaceae, are environmental organisms commonly found in soil, water, and the intestinal tracts of animals and humans. citrobacter koseri is known to be an uncommon but serious cause of both sporadic and epidemic septicemia and meningitis in neonates and young infants. most cases reported have occurred in immunocompromised hosts. the infections caused by citrobacter are difficult to treat with usual broad spectrum antibiotics owing to rapid g ... | 2015 | 26634165 |
| correlation of β-lactamase production and colistin resistance among enterobacteriaceae isolates from a global surveillance program. | the increasing use of carbapenems for treating multidrug-resistant (mdr) gram-negative bacterial infections has contributed to the global dissemination of carbapenem-resistant enterobacteriaceae (cre). serine and metallo-β-lactamases (mbls) that hydrolyze carbapenems have become prevalent and endemic in some countries, necessitating the use of older classes of agents, such as colistin. a total of 19,719 isolates of enterobacteriaceae (excluding proteeae and serratia spp., which have innate resis ... | 2016 | 26666920 |
| β-lactamase characterization of gram-negative pathogens recovered from patients enrolled in the phase 2 trials for ceftazidime-avibactam: clinical efficacies analyzed against subsets of molecularly characterized isolates. | the correlation of the clinical efficacies of ceftazidime-avibactam and comparators (carbapenems) was evaluated against baseline gram-negative isolates having characterized β-lactam resistance mechanisms from complicated urinary tract infection (cuti) and complicated intra-abdominal infection (ciai) phase 2 trials. enterobacteriaceae displaying ceftriaxone and/or ceftazidime mics of ≥ 2 μg/ml (69 isolates) and nonfermentative gram-negative bacilli (nf-gnb [three isolates]) with ceftazidime mics ... | 2016 | 26666936 |
| optimized use of the maldi biotyper system and the filmarray bcid panel for direct identification of microbial pathogens from positive blood cultures. | despite the current reliance on blood cultures (bcs), the diagnosis of bloodstream infections (bsis) can be sped up using new technologies performed directly on positive bc bottles. two methods (the maldi biotyper system and filmarray blood culture identification [bcid] panel) are potentially applicable. in this study, we performed a large-scale clinical evaluation (1,585 microorganisms from 1,394 bsi episodes) on the combined use of the maldi biotyper and filmarray bcid panel compared to a refe ... | 2016 | 26677254 |
| safe and effective deodorization of malodorous fungating tumors using topical metronidazole 0.75 % gel (gk567): a multicenter, open-label, phase iii study (rdt.07.sre.27013). | malignant fungating tumors are neoplastic tumors associated with skin ulcers, which are susceptible to microbial colonization. bacterial infection and proliferation may lead to malodor causing distress to patients. metronidazole-an effective agent against anaerobes-may contribute to deodorization and improvement in quality of life (qol). this study investigated the efficacy and safety of topical metronidazole 0.75 % gel for alleviation of malodor in anaerobically infected fungating neoplastic tu ... | 2015 | 26715293 |
| safe and effective deodorization of malodorous fungating tumors using topical metronidazole 0.75 % gel (gk567): a multicenter, open-label, phase iii study (rdt.07.sre.27013). | malignant fungating tumors are neoplastic tumors associated with skin ulcers, which are susceptible to microbial colonization. bacterial infection and proliferation may lead to malodor causing distress to patients. metronidazole-an effective agent against anaerobes-may contribute to deodorization and improvement in quality of life (qol). this study investigated the efficacy and safety of topical metronidazole 0.75 % gel for alleviation of malodor in anaerobically infected fungating neoplastic tu ... | 2015 | 26715293 |
| maldi-tof mass spectrometry: a powerful tool for clinical microbiology at hôpital principal de dakar, senegal (west africa). | our team in europe has developed the routine clinical laboratory identification of microorganisms by matrix-assisted laser desorption ionization time-of-flight (maldi-tof) mass spectrometry (ms). to evaluate the utility of maldi-tof ms in tropical africa in collaboration with local teams, we installed an apparatus in the hôpital principal de dakar (senegal), performed routine identification of isolates, and confirmed or completed their identification in france. in the case of discordance or a la ... | 2015 | 26716681 |
| ycgc represents a new protein deacetylase family in prokaryotes. | reversible lysine acetylation is one of the most important protein posttranslational modifications that plays essential roles in both prokaryotes and eukaryotes. however, only a few lysine deacetylases (kdacs) have been identified in prokaryotes, perhaps in part due to their limited sequence homology. herein, we developed a 'clip-chip' strategy to enable unbiased, activity-based discovery of novel kdacs in the escherichia coli proteome. in-depth biochemical characterization confirmed that ycgc i ... | 2015 | 26716769 |
| preclinical assessment of a fully automated multiplex pcr panel for detection of central nervous system pathogens. | we evaluated a multiplexed pcr panel for the detection of 16 bacterial, viral, and fungal pathogens in cerebrospinal fluid. panel results were compared to routine testing, and discrepancies were resolved by additional nucleic acid amplification tests or sequencing. overall, the positive and negative agreements across methods were 92.9% and 91.9%, respectively. | 2016 | 26719436 |
| evaluation of the filmarray blood culture identification panel: results of a multicenter controlled trial. | sepsis is a major cause of morbidity, mortality, and increased medical expense. rapid diagnosis improves outcomes and reduces costs. the filmarray blood culture identification panel (biofire diagnostics llc, salt lake city, ut), a highly multiplexed pcr assay, can identify 24 etiologic agents of sepsis (8 gram-positive, 11 gram-negative, and 5 yeast species) and three antimicrobial resistance genes (meca, vana/b, and blakpc) from positive blood culture bottles. it provides results in about 1 h w ... | 2016 | 26739158 |
| favorable outcome in cerebral abscesses caused by citrobacter koseri in a newborn infant. | the treatment of brain abscesses in newborn infants is controversial. we report on a 6-week-old infant with multiple brain abscesses caused by citrobacter koseri that resolved after treatment with combined surgical drainage and intravenous therapy with meropenem and fosfomycin. | 2014 | 26793443 |
| favorable outcome in cerebral abscesses caused by citrobacter koseri in a newborn infant. | the treatment of brain abscesses in newborn infants is controversial. we report on a 6-week-old infant with multiple brain abscesses caused by citrobacter koseri that resolved after treatment with combined surgical drainage and intravenous therapy with meropenem and fosfomycin. | 2014 | 26793443 |
| direct identification of urinary tract pathogens from urine samples, combining urine screening methods and matrix-assisted laser desorption ionization-time of flight mass spectrometry. | early diagnosis of urinary tract infections (utis) is essential to avoid inadequate or unnecessary empirical antibiotic therapy. microbiological confirmation takes 24 to 48 h. the use of screening methods, such as cytometry and automated microscopic analysis of urine sediment, allows the rapid prediction of negative samples. in addition, matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) is a widely established technique in clinical microbiology laborator ... | 2016 | 26818668 |
| a multi-substrate approach for functional metagenomics-based screening for (hemi)cellulases in two wheat straw-degrading microbial consortia unveils novel thermoalkaliphilic enzymes. | functional metagenomics is a promising strategy for the exploration of the biocatalytic potential of microbiomes in order to uncover novel enzymes for industrial processes (e.g. biorefining or bleaching pulp). most current methodologies used to screen for enzymes involved in plant biomass degradation are based on the use of single substrates. moreover, highly diverse environments are used as metagenomic sources. however, such methods suffer from low hit rates of positive clones and hence the dis ... | 2016 | 26822785 |
| microbial yield from physiotherapy assisted sputum production in respiratory outpatients. | sputum is a key diagnostic sample for those with chronic chest conditions including chronic and allergic aspergillus-related disease, but often not obtained in clinic. the objective of this study was to evaluate physiotherapeutic interventions to obtain sputum from those not able to spontaneously produce and the subsequent microbiological result. | 2016 | 26831895 |
| prevalence of urinary tract infection and antimicrobial susceptibility among diabetic patients with controlled and uncontrolled glycemia in kuwait. | diabetic patients have higher risk of urinary tract infection (uti). in the present study, we investigated the impact of glycemic control in diabetic patients on uti prevalence, type of strains, and their antimicrobial drugs susceptibility. this study was conducted on urine samples from 722 adult diabetic patients from which 252 (35%) samples were positive for uropathogens. most uti cases occurred in the uncontrolled glycemic group (197 patients) versus 55 patients with controlled glycemia. high ... | 2015 | 26844231 |
| prevalence of urinary tract infection and antimicrobial susceptibility among diabetic patients with controlled and uncontrolled glycemia in kuwait. | diabetic patients have higher risk of urinary tract infection (uti). in the present study, we investigated the impact of glycemic control in diabetic patients on uti prevalence, type of strains, and their antimicrobial drugs susceptibility. this study was conducted on urine samples from 722 adult diabetic patients from which 252 (35%) samples were positive for uropathogens. most uti cases occurred in the uncontrolled glycemic group (197 patients) versus 55 patients with controlled glycemia. high ... | 2015 | 26844231 |
| differences in the pharmacodynamics of ceftaroline against different species of enterobacteriaceae studied in an in vitro pharmacokinetic model of infection. | dose-ranging experiments were performed to study the pharmacodynamics of ceftaroline against enterobacteriaceae. | 2016 | 26846209 |
| antimicrobial activities of ceftaroline and comparator agents against bacterial organisms causing bacteremia in patients with skin and skin structure infections in u.s. medical centers, 2008 to 2014. | we evaluated the antimicrobial susceptibility of 1,454 organisms consecutively collected from patients with bacteremia associated with skin and skin structure infections. the most common organisms obtained werestaphylococcus aureus(670 organisms [46.1%]),escherichia coli(200 organisms [13.8%]), β-hemolytic streptococci (βhs) (138 organisms [9.5%]), andklebsiella pneumoniae(109 organisms [7.5%]). the susceptibility rates for ceftaroline were 97.9% fors. aureus(95.9% among methicillin-resistants. ... | 2016 | 26856825 |
| persistence of nasal colonization with human pathogenic bacteria and associated antimicrobial resistance in the german general population. | the nares represent an important bacterial reservoir for endogenous infections. this study aimed to assess the prevalence of nasal colonization by different important pathogens, the associated antimicrobial susceptibility and risk factors. we performed a prospective cohort study among 1878 nonhospitalized volunteers recruited from the general population in germany. participants provided nasal swabs at three time points (each separated by 4-6 months). staphylococcus aureus, enterobacteriaceae and ... | 2015 | 26862431 |
| persistence of nasal colonization with human pathogenic bacteria and associated antimicrobial resistance in the german general population. | the nares represent an important bacterial reservoir for endogenous infections. this study aimed to assess the prevalence of nasal colonization by different important pathogens, the associated antimicrobial susceptibility and risk factors. we performed a prospective cohort study among 1878 nonhospitalized volunteers recruited from the general population in germany. participants provided nasal swabs at three time points (each separated by 4-6 months). staphylococcus aureus, enterobacteriaceae and ... | 2015 | 26862431 |
| restriction profiling of 23s microheterogenic ribosomal repeats for detection and characterizing of e. coli and their clonal, pathogenic, and phylogroups. | correlating ribosomal microheterogenicity with unique restriction profiles can prove to be an efficacious and cost-effective approach compared with sequencing for microbial identification. an attempt to peruse restriction profiling of 23s ribosomal assemblage was ventured; digestion patterns with bfa i discriminated e. coli from its colony morphovars, while hae iii profiles assisted in establishing distinct clonal groups. among the gene pool of 399 ribosomal sequences extrapolated from 57 e. col ... | 2015 | 26885397 |
| evaluation of the check-points check mdr ct103 and ct103 xl microarray kits by use of preparatory rapid cell lysis. | using a rapid bacterial lysis method, the check mdr ct103 and ct103 xl microarrays demonstrated accuracies of 98.1% and 94.2%, respectively, for detection of known resistance genes in 108 multidrug-resistant gram-negative bacilli. in 45 isolates, 49 previously unrecognized extended-spectrum β-lactamase or plasmid ampc targets were detected and confirmed by conventional pcr. | 2016 | 26888905 |
| the basics of bacteriuria: strategies of microbes for persistence in urine. | bacteriuria, the presence of bacteria in urine, is associated with asymptomatic, as well as symptomatic, urinary tract infection (uti). bacteriuria underpins some of the dynamics of microbial colonization of the urinary tract, and probably impacts the progression and persistence of infection in some individuals. recent molecular discoveries in vitro have elucidated how some key bacterial traits can enable organisms to survive and grow in human urine as a means of microbial fitness adaptation for ... | 2016 | 26904513 |
| review on the antimicrobial resistance of pathogens from tracheal and endotracheal aspirates of patients with clinical manifestations of pneumonia in bacolod city in 2013. | microbiological content specifically bacterial and fungal etiologies from tracheal aspirates in a tertiary hospital in bacolod city was reviewed for baseline information. a total of 130 tracheal aspirates were subjected for culture to isolate and identify the pathogen and determine their susceptibilities to various antibiotics. productions of certain enzymes responsible for antibiotic resistance like esbl (extended spectrum beta-lactamase), metallo-β-lactamase, and carbapenemase were also studie ... | 2015 | 26904750 |
| matrix-assisted laser desorption ionization-time of flight mass spectrometry accelerates pathogen identification and may confer benefit in the outcome of peritoneal dialysis-related peritonitis. | matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) and conventional standard methods were compared for time to pathogen identification and impact on clinical outcomes in peritoneal dialysis-related peritonitis patients. the maldi-tof ms method identified the causative microorganisms earlier (average time saved, 64 h for all pathogens), and patients had a shorter hospital stay (mean ± standard deviation, 5.2 ± 4.8 days versus 8.2 ± 4.5 days, p = 0.001). | 2016 | 26912750 |
| development of a flow cytometry-based method for rapid detection of escherichia coli and shigella spp. using an oligonucleotide probe. | standard methods to detect escherichia coli contamination in food use the polymerase chain reaction (pcr) and agar culture plates. these methods require multiple incubation steps and take a long time to results. an improved rapid flow-cytometry based detection method was developed, using a fluorescence-labeled oligonucleotide probe specifically binding a16s rrna sequence. the method positively detected 51 e. coli isolates as well as 4 shigella species. all 27 non-e. coli strains tested gave nega ... | 2016 | 26913737 |
| in vitro susceptibility to ceftazidime-avibactam of carbapenem-nonsusceptible enterobacteriaceae isolates collected during the inform global surveillance study (2012 to 2014). | the activity of ceftazidime-avibactam was assessed against 961 isolates of meropenem-nonsusceptible enterobacteriaceae most meropenem-nonsusceptible metallo-β-lactamase (mbl)-negative isolates (97.7%) were susceptible to ceftazidime-avibactam. isolates that carried kpc or oxa-48-like β-lactamases, both alone and in combination with extended-spectrum β-lactamases (esbls) and/or ampc β-lactamases, were 98.7% and 98.5% susceptible to ceftazidime-avibactam, respectively. meropenem-nonsusceptible, ca ... | 2016 | 26926648 |
| [identification of microorganisms related to chronic rhinosinusitis in adult patients with variable common immunodeficiency]. | the prevalence of chronic rhinosinusitis in adult patients with common variable immunodeficiency (cvid) is 52%. the patients with cvid show higher incidence of chronic rhinosinusitis, which is an inflammatory disease that affects the lining of one or more paranasal sinuses and nasal cavity. | 2016 | 26943827 |
| phenotypic detection and molecular characterization of beta-lactamase genes among citrobacter species in a tertiary care hospital. | to examine the distribution, emergence, and spread of genes encoding beta-lactamase resistance in citrobacter species isolated from hospitalized patients in a tertiary care hospital. | 2016 | 26952135 |
| microbiology of primary acquired nasolacrimal duct obstruction: simple epiphora, acute dacryocystitis, and chronic dacryocystitis. | the aim of this study was to determine the microbiology of primary acquired nasolacrimal duct obstruction (pando) and its antimicrobial susceptibilities. | 2016 | 26955261 |
| pancreatic panniculitis complicated by infection with corynebacterium tuberculostearicum: a case report. | we present a case of pancreatic panniculitis in a patient with alcohol abuse where corynebacterium tuberculostearicum was isolated from a pannicular nodule on the crus. the patient was started on linezolid treatment leading to regression of the patient's symptoms. upon discontinuation of linezolid treatment progression of the skin symptoms progressed. | 2014 | 26955524 |
| evaluation of the septifast mgrade test on standard care wards--a cohort study. | the immediate need for appropriate antimicrobial therapy in septic patients requires the detection of the causative pathogen in a timely and reliable manner. in this study, the real-time pcr septifast mgrade test was evaluated in adult patients meeting the systemic inflammatory response syndrome (sirs) criteria that were treated at standard care wards. | 2016 | 26986826 |
| cefepime shows good efficacy and no antibiotic resistance in pneumonia caused by serratia marcescens and proteus mirabilis - an observational study. | many antibiotics have no effect on gram-positive and gram-negative microbes, which necessitates the prescription of broad-spectrum antimicrobial agents that can lead to increased risk of antibiotic resistance. these pathogens constitute a further threat because they are also resistant to numerous beta-lactam antibiotics, as well as other antibiotic groups. this study retrospectively investigates antimicrobial resistance in hospitalized patients suffering from pneumonia triggered by gram-negative ... | 2016 | 27004519 |
| insights into newer antimicrobial agents against gram-negative bacteria. | currently, drug resistance, especially against cephalosporins and carbapenems, among gram-negative bacteria is an important challenge, which is further enhanced by the limited availability of drugs against these bugs. there are certain antibiotics (colistin, fosfomycin, temocillin, and rifampicin) that have been revived from the past to tackle the menace of superbugs, including members of enterobacteriaceae, acinetobacter species, and pseudomonas species. very few newer antibiotics have been add ... | 2016 | 27013887 |
| genomic insights into a new citrobacter koseri strain revealed gene exchanges with the virulence-associated yersinia pestis ppcp1 plasmid. | the history of infectious diseases raised the plague as one of the most devastating for human beings. far too often considered an ancient disease, the frequent resurgence of the plague has led to consider it as a reemerging disease in madagascar, algeria, libya, and congo. the genetic factors associated with the pathogenicity of yersinia pestis, the causative agent of the plague, involve the acquisition of the ppcp1 plasmid that promotes host invasion through the expression of the virulence fact ... | 2016 | 27014253 |
| fatal transfusion-transmitted infection due to citrobacter koseri. | transfusion-transmitted bacterial infection (ttbi) is still one of the most feared complications of blood transfusion. | 2016 | 27040329 |
| global rna recognition patterns of post-transcriptional regulators hfq and csra revealed by uv crosslinking in vivo. | the molecular roles of many rna-binding proteins in bacterial post-transcriptional gene regulation are not well understood. approaches combining in vivo uv crosslinking with rna deep sequencing (clip-seq) have begun to revolutionize the transcriptome-wide mapping of eukaryotic rna-binding protein target sites. we have applied clip-seq to chart the target landscape of two major bacterial post-transcriptional regulators, hfq and csra, in the model pathogen salmonella typhimurium. by detecting bind ... | 2016 | 27044921 |
| post-stroke bacteriuria among stroke patients attending a physiotherapy clinic in ghana: a cross-sectional study. | infections are known to be a major complication of stroke patients. in this study, we evaluated the risk of community-acquired bacteriuria among stroke patients, the associated factors, and the causative organisms. | 2016 | 27051289 |
| noma: a disease of poverty presenting at an urban hospital in the united states. | 2014 | 27075129 | |
| noma: a disease of poverty presenting at an urban hospital in the united states. | 2014 | 27075129 | |
| noncanonical dna-binding mode of repressor and its disassembly by antirepressor. | dna-binding repressors are involved in transcriptional repression in many organisms. disabling a repressor is a crucial step in activating expression of desired genes. thus, several mechanisms have been identified for the removal of a stably bound repressor (rep) from the operator. here, we describe an uncharacterized mechanism of noncanonical dna binding and induction by a rep from the temperate salmonella phage spc32h; this mechanism was revealed using the crystal structures of homotetrameric ... | 2016 | 27099293 |
| antimicrobial activities of ceftazidime-avibactam and comparator agents against gram-negative organisms isolated from patients with urinary tract infections in u.s. medical centers, 2012 to 2014. | a total of 7,272 unique patient clinical isolates were collected from 71 u.s. medical centers from patients with urinary tract infections in 2012 to 2014 and tested for susceptibility to ceftazidime-avibactam and comparators by broth microdilution methods. ceftazidime-avibactam inhibited >99.9% of all enterobacteriaceae at the susceptible breakpoint of ≤8 μg/ml (there were only three nonsusceptible strains). ceftazidime-avibactam was also active against pseudomonas aeruginosa isolates (mic50, 2 ... | 2016 | 27114273 |
| global dissemination of blakpc into bacterial species beyond klebsiella pneumoniae and in vitro susceptibility to ceftazidime-avibactam and aztreonam-avibactam. | the klebsiella pneumoniae carbapenemase (kpc), first described in the united states in 1996, is now a widespread global problem in several gram-negative species. a worldwide surveillance study collected gram-negative pathogens from 202 global sites in 40 countries during 2012 to 2014 and determined susceptibility to β-lactams and other class agents by broth microdilution testing. molecular mechanisms of β-lactam resistance among carbapenem-nonsusceptible enterobacteriaceae and pseudomonas aerugi ... | 2016 | 27161636 |
| epidemiology and resistance patterns of bacterial and fungal colonization of biliary plastic stents: a prospective cohort study. | plastic stents used for the treatment of biliary obstruction will become occluded over time due to microbial colonization and formation of biofilms. treatment of stent-associated cholangitis is often not effective because of inappropriate use of antimicrobial agents or antimicrobial resistance. we aimed to assess the current bacterial and fungal etiology of stent-associated biofilms, with particular emphasis on antimicrobial resistance. | 2016 | 27171497 |
| [prevalence of sensitization to allergens in school children with asthma residents from guadalajara metropolitan area]. | allergic sensitization is dependent on the geographical and climatic conditions in each region; therefore, identify agents most commonly sensitized children with asthma is important for planning prevention measures. | 2016 | 27174756 |
| [prevalence of sensitization to fungi in patients with respiratory allergy]. | as part of the etiology of respiratory allergy we have genetics, prenatal factors and sensitivity to various airborne allergens, between these fungi are found. relationship has been found between sensitization to fungal in skin tests and allergy pathogenesis and aggravation. there is a few literature in mexico and in the north of the country it is lacking regarding this problem. | 2016 | 27174757 |
| isolation and characterization of escherichia coli sequence type 131 and other antimicrobial-resistant gram-negative bacilli from clinical stool samples from veterans. | emerging multidrug-resistant (mdr) gram-negative bacilli (gnb), including escherichia coli sequence type 131 (st131) and its resistance-associated h30 subclone, constitute an ever-growing public health threat. their reservoirs and transmission pathways are incompletely defined. to assess diarrheal stools as a potential reservoir for st131-h30 and other mdr gnb, we cultured 100 clinical stool samples from a veterans affairs medical center clinical laboratory (october to december 2011) for fluoroq ... | 2016 | 27185805 |
| evaluation of heavy metal removal from wastewater in a modified packed bed biofilm reactor. | for the effective application of a modified packed bed biofilm reactor (pbbr) in wastewater industrial practice, it is essential to distinguish the tolerance of the system for heavy metals removal. the industrial contamination of wastewater from various sources (e.g. zn, cu, cd and ni) was studied to assess the impacts on a pbbr. this biological system was examined by evaluating the tolerance of different strengths of composite heavy metals at the optimum hydraulic retention time (hrt) of 2 hour ... | 2016 | 27186636 |
| clinical impact of maldi-tof ms identification and rapid susceptibility testing on adequate antimicrobial treatment in sepsis with positive blood cultures. | shortening the turn-around time (tat) of positive blood culture (bc) identification (id) and susceptibility results is essential to optimize antimicrobial treatment in patients with sepsis. we aimed to evaluate the impact on antimicrobial prescription of a modified workflow of positive bcs providing id and partial susceptibility results for enterobacteriaceae (eb), pseudomonas aeruginosa and staphylococcus aureus on the day of bc positivity detection. this study was divided into a pre-interventi ... | 2016 | 27228001 |
| acute rhinosinusitis and intraorbital abscess caused by citrobacter koseri infection. | 2016 | 27245934 | |
| thigh abscess and necrotizing fasciitis following an inside-out transobturator tape intervention: a case report. | tension-free vaginal transobturator tapes are used worldwide in the treatment of urinary incontinence in women. very few severe complications have been described following this procedure, with no standard treatment yet established. | 2016 | 27256334 |
| vector potential of blattella germanica (l.) (dictyoptera: blattidae) for medically important bacteria at food handling establishments in jimma town, southwest ethiopia. | cockroaches have been regarded as possible vectors of human enteropathogens. their presence and crawl particularly in food handling establishments could be risky for human health. therefore, this study was done to determine the vector potential of cockroach for medically important bacterial pathogens in restaurants and cafeterias. a cross-sectional study was conducted on cockroaches from restaurants and cafeterias in jimma town from may to september 2014. standard taxonomic keys and microbiologi ... | 2016 | 27294115 |
| early-onset pneumonia in non-traumatic out-of-hospital cardiac arrest patients with special focus on prehospital airway management. | background more than half of all non-traumatic out-of-hospital cardiac arrest (ohca) patients die in the hospital. early-onset pneumonia (eop) has been described as one of the most common complications after successful cardiopulmonary resuscitation. however, the expanded use of alternative airway devices (aad) might influence the incidence of eop following ohca. material and methods we analyzed data from all ohca patients admitted to our hospital between 1 january 2008 and 31 december 2014. eop ... | 2016 | 27295123 |
| occurrence of potential bacterial pathogens and their antimicrobial susceptibility patterns isolated from herbal medicinal products sold in different markets of gondar town, northwest ethiopia. | background. the world health organization estimates that about 80% of the world's population uses herbal medicine to treat various illnesses as means of primary healthcare. however, during preparation, herbal plants may be exposed to contamination by potential pathogens, and this may lead to infections. the aim of this study was to determine bacterial contamination of herbal medicinal products and to assess the antibiotic susceptibility pattern of the isolated bacteria. methods. a cross-sectiona ... | 2016 | 27299154 |
| recent advances in screening of anti-campylobacter activity in probiotics for use in poultry. | campylobacteriosis is the most common cause of bacterial gastroenteritis worldwide. campylobacter species involved in this infection usually include the thermotolerant species campylobacter jejuni. the major reservoir for c. jejuni leading to human infections is commercial broiler chickens. poultry flocks are frequently colonized by c. jejuni without any apparent symptoms. risk assessment analyses have identified the handling and consumption of poultry meat as one of the most important sources o ... | 2016 | 27303366 |
| ceftazidime-avibactam versus doripenem for the treatment of complicated urinary tract infections, including acute pyelonephritis: recapture, a phase 3 randomized trial program. | the global emergence of carbapenem-resistant enterobacteriaceae highlights the urgent need to reduce carbapenem dependence. the phase 3 recapture program compared the efficacy and safety of ceftazidime-avibactam and doripenem in patients with complicated urinary tract infection (cuti), including acute pyelonephritis. | 2016 | 27313268 |
| presence of gas in left ventricle due to infective endocarditis. | gas in myocardium is a rare manifestation of infective endocarditis caused by gas producing bacteria. we present a case of infective endocarditis caused by citrobacter koseri initially diagnosed by computed tomography and confirmed with transesophageal echocardiogram. | 2016 | 27318588 |
| strategies used by bacteria to grow in macrophages. | intracellular bacteria are often clinically relevant pathogens that infect virtually every cell type found in host organisms. however, myeloid cells, especially macrophages, constitute the primary cells targeted by most species of intracellular bacteria. paradoxically, macrophages possess an extensive antimicrobial arsenal and are efficient at killing microbes. in addition to their ability to detect and signal the presence of pathogens, macrophages sequester and digest microorganisms using the p ... | 2016 | 27337444 |
| temporal trends and risks factors for antimicrobial resistant enterobacteriaceae urinary isolates from outpatients in guadeloupe. | urinary tract infections are bacterial infections most commonly encountered in the community. the resistance rate of uropathogens to commonly prescribed antibiotics has increased worldwide but there are no published data concerning the resistance of strains isolated from community-acquired uti in guadeloupe. to assess the susceptibility patterns of enterobacteriaceae strains isolated from outpatients in guadeloupe we conducted a prospective study from december 2012 to may 2014 among outpatients ... | 2016 | 27342199 |
| an 80-year-old woman surgically treated for native valve infective endocarditis caused by citrobacter koseri. | 2017 | 27345426 | |
| occurrence of enterobacteriaceae in raw meat and in human samples from egyptian retail sellers. | the present study was performed to assess the presence of enterobacteriaceae in raw meat and handlers in egypt using cultivation and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms). a total of 100 raw meat samples (chicken and beef meat, 50 each) were randomly purchased from butchers and local meat retailers located at mansoura city, egypt. fifty human samples were collected from meat handlers (hand swabs and stool specimens, 25 each). 228 bacterial is ... | 2014 | 27379312 |
| high prevalence of extended-spectrum ß-lactamase producing enterobacteriaceae among clinical isolates in burkina faso. | nothing is known about the epidemiology and resistance mechanisms of extended-spectrum ß-lactamase-producing enterobacteriaceae (esbl-pe) in burkina faso. the objective of this study was to determine esbl-pe prevalence and to characterize esbl genes in burkina faso. | 2016 | 27400864 |
| comparative study of cronobacter identification according to phenotyping methods. | microbiological criteria applied to powdered infant formula (pif) require the absence of all cronobacter spp. consequently, misidentification of isolates from finished products can lead to significant financial losses for manufacturers and could increase the risk of neonatal infection. biochemical identification of suspect isolates using commercially available test panels is recommended for use by pif manufacturers by both the us fda and iso standard methods for cronobacter species; however, phe ... | 2016 | 27401027 |
| senca: a multilayered codon model to study the origins and dynamics of codon usage. | gene sequences are the target of evolution operating at different levels, including the nucleotide, codon, and amino acid levels. disentangling the impact of those different levels on gene sequences requires developing a probabilistic model with three layers. here we present senca (site evolution of nucleotides, codons, and amino acids), a codon substitution model that separately describes 1) nucleotide processes which apply on all sites of a sequence such as the mutational bias, 2) preferences ... | 2016 | 27401173 |
| the target spectrum of sdsr small rna in salmonella. | model enteric bacteria such as escherichia coli and salmonella enterica express hundreds of small non-coding rnas (srnas), targets for most of which are yet unknown. some srnas are remarkably well conserved, indicating that they serve cellular functions that go beyond the necessities of a single species. one of these 'core srnas' of largely unknown function is the abundant ∼100-nucleotide sdsr srna which is transcribed by the general stress σ-factor, σ(s) and accumulates in stationary phase. in ... | 2016 | 27407104 |
| musculoskeletal infections associated with citrobacter koseri. | introduction citrobacter koseri is a well known cause of central nervous system infections in the paediatric setting. musculoskeletal infections caused by c koseri are rare, with only 14 previously reported cases. we present the first recorded case of c koseri induced septic arthritis of the knee along with a review of the literature. methods a search of the pubmed, embase(®) and google scholar™ databases was undertaken. only complete or near complete cases were reviewed. findings fourteen muscu ... | 2016 | 27412805 |
| interferon-inducible guanylate-binding proteins at the interface of cell-autonomous immunity and inflammasome activation. | guanylate-binding proteins (gbps) are essential components of cell-autonomous immunity. in response to ifn signaling, gbps are expressed in the cytoplasm of immune and nonimmune cells, where they unleash their antimicrobial activity toward intracellular bacteria, viruses, and parasites. recent studies have revealed that gbps are essential for mediating activation of the caspase-1 inflammasome in response to the gram-negative bacteria salmonella enterica serovar typhimurium, francisella novicida, ... | 2017 | 27418355 |
| prevalence of extended spectrum beta-lactamase production among uropathogens in south mumbai and its antibiogram pattern. | β-lactams are the most widely used group of antimicrobials. however, increasing resistance to these valuable drugs in uropathogens, mediated principally by β-lactamases, has become a major concern. the present study was undertaken to determine the prevalence of extended spectrum β-lactamase (esbl) producers in clinical isolates of urine specimens, collected from various healthcare centres across south mumbai. a total of 195 gram negative urine isolates were identified as pseudomonas aeruginosa ( ... | 2012 | 27418912 |
| development of a phage cocktail to control proteus mirabilis catheter-associated urinary tract infections. | proteus mirabilis is an enterobacterium that causes catheter-associated urinary tract infections (cautis) due to its ability to colonize and form crystalline biofilms on the catheters surface. cautis are very difficult to treat, since biofilm structures are highly tolerant to antibiotics. phages have been used widely to control a diversity of bacterial species, however, a limited number of phages for p. mirabilis have been isolated and studied. here we report the isolation of two novel virulent ... | 2016 | 27446059 |
| a bioinformatics analysis reveals a group of mocr bacterial transcriptional regulators linked to a family of genes coding for membrane proteins. | the mocr bacterial transcriptional regulators are characterized by an n-terminal domain, 60 residues long on average, possessing the winged-helix-turn-helix (whth) architecture responsible for dna recognition and binding, linked to a large c-terminal domain (350 residues on average) that is homologous to fold type-i pyridoxal 5'-phosphate (plp) dependent enzymes like aspartate aminotransferase (aat). these regulators are involved in the expression of genes taking part in several metabolic pathwa ... | 2016 | 27446613 |
| characteristics of ciprofloxacin-resistant enterobacteriaceae isolates recovered from wastewater of an algerian hospital. | hospital effluents are a source of environmental pollution by drugs, antibiotic-resistant bacteria, and resistance genes. quinolones, particularly ciprofloxacin, are commonly detected in these effluents, contributing to the emergence of antimicrobial resistance. the objective of this study was to characterize ciprofloxacin-resistant enterobacteriaceae in hospital effluents. | 2016 | 27482804 |
| effect of 4 % chlorhexidine on cord colonization among hospital and community births in india: a randomized controlled study. | infections are the single most important cause of neonatal mortality in developing countries. results from trials in asia evaluating the effect of chlorhexidine on neonatal mortality have been encouraging but limited data are available on the impact of cord cleansing on bacterial colonization. further, no data from facility deliveries and impact with time is available. this pilot study was aimed to evaluate the impact of 4 % commercially prepared chlorhexidine on cord colonization and density of ... | 2016 | 27484013 |
| assessment of the effectiveness of three different cephalosporins/clavulanate combinations for the phenotypic confirmation of extended spectrum beta lactamases producer bacterial isolates from urine samples at national public health laboratory, kathmandu, nepal. | the extended-spectrum β-lactamase (esbl) producing bacteria are present as the serious public health problems due to their resistance to large number of antibiotics. the main aims of this study were to determine the prevalence and antibiotic resistance patterns of bacteria producing extended-spectrum β-lactamases (esbls) and to find the suitable cephalosporin/clavulanate combination for phenotypic confirmation of esbl production. | 2016 | 27488224 |
| determinants of deescalation failure in critically ill patients with sepsis: a prospective cohort study. | introduction. deescalation refers to either discontinuation or a step-down of antimicrobials. despite strong recommendations in the surviving sepsis guidelines (2012) to deescalate, actual practices can vary. our objective was to identify variables that are associated with deescalation failure. methods. in this prospective study of patients with sepsis/septic shock, patients were categorized into 4 groups based on antibiotic administration: no change in antibiotics, deescalation, escalation (whe ... | 2016 | 27493799 |
| elevated plasma matrix metalloproteinase-9 and its correlations with severity of disease in patients with ventilator-associated pneumonia. | ventilator-associated pneumonia (vap) increases patient mortality and medical expenditure, and a real-time and reliable method for the rapid diagnosis of vap may help reduce fatal complications. matrix metalloproteinases-9 (mmp-9) is considered significant in the pathogenesis of lung inflammation and infection. therefore, we examined its relationship with the clinical course of vap. this retrospective observational study recruited 30 healthy volunteers, 12 patients who used mechanical ventilatio ... | 2016 | 27499696 |
| risk factors to predict drug-resistant pathogens in hemodialysis-associated pneumonia. | after the concept of healthcare associated pneumonia (hcap) was introduced in 2005 by the american thoracic society/infectious disease society of america (ats/idsa), pneumonia in hemodialysis patients has been classified as hcap. even though there are several risk factors and scoring systems of drug-resistant pathogens (drps) in hcap, the risk factors for drps in hemodialysis-associated pneumonia are unclear. | 2016 | 27502599 |
| high-density lipoprotein binds to mycobacterium avium and affects the infection of thp-1 macrophages. | high-density lipoprotein (hdl) is involved in innate immunity toward various infectious diseases. concerning bacteria, hdl is known to bind to lipopolysaccharide (lps) and to neutralize its physiological activity. on the other hand, cholesterol is known to play an important role in mycobacterial entry into host cells and in survival in the intracellular environment. however, the pathogenicity of mycobacterium avium (m. avium) infection, which tends to increase worldwide, remains poorly studied. ... | 2016 | 27516907 |
| antibiotic utilization patterns in patients with ventilator-associated pneumonia: a canadian context. | this retrospective cohort study describes the patterns of antibiotic use for the treatment of ventilator-associated pneumonia (vap) in the calgary zone of alberta health services. timing, appropriateness, and duration of antibiotics were evaluated in two hundred consecutive cases of vap derived from 4 adult intensive care units (icu). antibiotic therapy was initiated in less than 24 hours from vap diagnosis in 83% of cases. although most patients (89%) received empiric therapy that demonstrated ... | 2016 | 27525016 |
| systematic review: adverse events of fecal microbiota transplantation. | fecal microbiota transplantation (fmt) is a microbiota-based therapy that shows therapeutic potential in recurrent or refractory clostridium difficile infections and other intestinal or extra-intestinal disorders. nonetheless, adverse events (aes) remain a major challenge in the application of fmt. | 2016 | 27529553 |
| fluoroquinolone-resistant and extended-spectrum β-lactamase-producing escherichia coli infections in patients with pyelonephritis, united states(1). | for 2013-2014, we prospectively identified us adults with flank pain, temperature >38.0°c, and a diagnosis of acute pyelonephritis, confirmed by culture. cultures from 453 (86.9%) of 521 patients grew escherichia coli. among e. coli isolates from 272 patients with uncomplicated pyelonephritis and 181 with complicated pyelonephritis, prevalence of fluoroquinolone resistance across study sites was 6.3% (range by site 0.0%-23.1%) and 19.9% (0.0%-50.0%), respectively; prevalence of extended-spectrum ... | 2016 | 27532362 |