Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| cloning and characterization of mupp1, a novel pdz domain protein. | using the yeast two-hybrid system we isolated a cdna clone encoding a novel protein interacting with the c-terminal domain of the 5-ht2c receptor. the protein, named mupp1 (multi-pdz-domain protein), contains thirteen pdz domains and no obvious catalytic domain; it is related to hinadl and a putative c. elegans polypeptide referred to as c52a11.4 containing six or ten pdz domains, respectively. domains highly similar to those of mupp1 are arrayed in the same order in all three proteins. the mupp ... | 1998 | 9537516 |
| chloroform and carbon tetrachloride induce intrachromosomal recombination and oxidative free radicals in saccharomyces cerevisiae. | chlorination of drinking water results in the generation of low levels of numerous chlorinated hydrocarbons due to the reaction of chlorine with naturally occurring organic compounds in the water. concern has been raised about the safety of these chlorinated contaminants as several of them, most notably chloroform (trichloromethane), have been shown to be carcinogenic in long-term rodent bioassays and weak correlations between trihalomethane levels in drinking water and an increased risk of blad ... | 1998 | 9541652 |
| preclinical enantioselective pharmacology of (r)- and (s)- ketorolac. | many of the nonsteroidal anti-inflammatory drugs (nsaids) are marketed as racemic mixtures, composed of (r)- and (s)- enantiomers. racemic nsaids are potent cyclooxygenase (cox) inhibitors only through the action of the (s)- enantiomers, as the (r)- enantiomers do not exhibit cox inhibition. however, the (r)- enantiomer of ketoprofen exhibits potent analgesic activity and minimal ulcerogenic potential. to extend these observations, we examined the (r)- and (s)- enantiomers of rs- ketorolac, (s)- ... | 1998 | 9549656 |
| a phosphatidylinositol 3-kinase and phosphatidylinositol transfer protein act synergistically in formation of constitutive transport vesicles from the trans-golgi network. | current evidence suggests that phosphatidylinositol (pi) kinases and phosphatidylinositol transfer protein (pitp) are involved in driving vesicular traffic from yeast and mammalian trans-golgi network (tgn). we have tested the interaction between these cytosolic proteins in an assay that measures the formation of constitutive transport vesicles from the tgn in a hepatocyte cell-free system. this reaction is dependent on a novel pi 3-kinase, and we now report that, under conditions of limiting cy ... | 1998 | 9553090 |
| the list of cytoplasmic ribosomal proteins of saccharomyces cerevisiae. | screening of the complete genome sequence from the yeast saccharomyces cerevisiae has enabled us to compile a complete list of the genes encoding cytoplasmic ribosomal proteins in this organism. putative ribosomal protein genes were selected primarily on the basis of the sequence similarity of their products with ribosomal proteins from other eukaryotic organisms, in particular the rat. these genes were subsequently screened for typical yeast rp-gene characteristics, viz. (1) a high codon adapta ... | 1998 | 9559554 |
| characterization of delta 9 acyl-lipid desaturase homologues from arabidopsis thaliana. | two cdnas, ads1 and ads2, were isolated from arabidopsis. these cdnas encoded proteins homologous to delta 9 acyl-lipid desaturases of cyanobacteria and acyl-coa desaturases of yeast and mammals. the expression of ads1 and ads2 was organ-dependent. cold temperature up-regulated the ads2 expression, whereas it down-regulated the ads1 expression. | 1998 | 9559566 |
| cell cycle arrest in cdc20 mutants of saccharomyces cerevisiae is independent of ndc10p and kinetochore function but requires a subset of spindle checkpoint genes. | the spindle checkpoint ensures accurate chromosome segregation by inhibiting anaphase onset in response to altered microtubule function and impaired kinetochore function. in this study, we report that the ability of the anti-microtubule drug nocodazole to inhibit cell cycle progression in saccharomyces cerevisiae depends on the function of the kinetochore protein encoded by ndc10. we examined the role of the spindle checkpoint in the arrest in cdc20 mutants that arrest prior to anaphase with an ... | 1998 | 9560388 |
| differential production of chemokines by phagocytosing rat neutrophils and macrophages. | in this study, rat neutrophils and macrophages produced cytokine-induced neutrophil chemoattractants (cincs) and rat macrophage inflammatory protein-1 alpha (mip-1 alpha) in different patterns during phagocytosis of heat-killed yeast cells in vitro. the cultured supernatants of the phagocytosing rat neutrophils and macrophages had chemotactic activities toward neutrophils, and the chemotactic potencies were markedly inhibited by anti-cincs iggs or/and anti-mip-1 alpha igg, suggesting that cincs ... | 1998 | 9561925 |
| efficient large-scale transformation of yeast. | 1998 | 9564532 | |
| identification and functional characterization of a novel human protein highly related to the yeast dynamin-like gtpase vps1p. | dynamin proteins containing a gtpase domain, a pleckstrin homology motif and a proline-rich tail participate in receptor-mediated endocytosis in organisms ranging from insects to vertebrates. in addition, dynamin-related gtpases, such as the yeast golgi protein vps1p, which lack both the pleckstrin homology motif and the proline-rich region, participate in vesicular transport within the secretory pathway in lower eukaryotes. however, no data is available on the existence of vps1p-like proteins i ... | 1998 | 9570752 |
| structure of the calcium pump from sarcoplasmic reticulum at 8-a resolution. | the calcium pump from sarcoplasmic reticulum (ca2+-atpase) is typical of the large family of p-type cation pumps. these couple atp hydrolysis with cation transport, generating cation gradients across membranes. ca2+-atpase specifically maintains the low cytoplasmic calcium concentration of resting muscle by pumping calcium into the sarcoplasmic reticulum; subsequent release is used to initiate contraction. no high-resolution structure of a p-type pump has yet been determined, although a 14-a str ... | 1998 | 9572145 |
| the human ril gene: mapping to human chromosome 5q31.1, genomic organization and alternative transcripts. | the ril gene encoding a lim domain protein of an unknown function was previously identified by differential expression cloning as a candidate tumor suppressor gene in rat fibroblasts (kiess, m., scharm, b., aguzzi, a., hajnal, a., klemenz, r., schwarte-waldhoff, i., schafer, r., 1995. expression of ril, a novel lim domain gene, is down-regulated in hras-transformed cells and restored in phenotypic revertants. oncogene 10, 61-68). searching for novel genes on human chromosome 5q31.1 by the cdna s ... | 1998 | 9573374 |
| syngap: a synaptic rasgap that associates with the psd-95/sap90 protein family. | the psd-95/sap90 family of proteins has recently been implicated in the organization of synaptic structure. here, we describe the isolation of a novel ras-gtpase activating protein, syngap, that interacts with the pdz domains of psd-95 and sap102 in vitro and in vivo. syngap is selectively expressed in brain and is highly enriched at excitatory synapses, where it is present in a large macromolecular complex with psd-95 and the nmda receptor. syngap stimulates the gtpase activity of ras, suggesti ... | 1998 | 9581761 |
| identification of mouse ulk1, a novel protein kinase structurally related to c. elegans unc-51. | a novel protein kinase related to the c. elegans serine/threonine kinase unc-51 was cloned from mouse. the unc-51-like kinase (ulk)1 is encoded by a cdna of 1051 amino acids with calculated mw of 113 kda. comparison of the ulk1 and unc-51 shows the highest conservation in the amino-terminal kinase domain, which is followed by a proline/serine-rich (ps) domain and a conserved carboxyl-terminal (c) domain. ulk1 mrna is expressed in various tissues, and is mapped to mouse chromosome 5f and rat chro ... | 1998 | 9600096 |
| the lissencephaly gene product lis1, a protein involved in neuronal migration, interacts with a nuclear movement protein, nudc. | important clues to how the mammalian cerebral cortex develops are provided by the analysis of genetic diseases that cause cortical malformations [1-5]. people with miller-dieker syndrome (mds) or isolated lissencephaly sequence (ils) have a hemizygous deletion or mutation in the lis1 gene [3,6]; both conditions are characterized by a smooth cerebral surface, a thickened cortex with four abnormal layers, and misplaced neurons [7,8]. lis1 is highly expressed in the ventricular zone and the cortica ... | 1998 | 9601647 |
| ribonucleases from rat and bovine liver: purification, specificity and structural characterization. | the presence of four members of the pyrimidine-specific ribonuclease superfamily was demonstrated in rat liver. three of them (rl1, rl2 and rl3) were purified and showed ribonuclease activity at ph 7.5 with yeast rna as substrate. rl1 is identical to rat pancreatic ribonuclease (ribonuclease 1). n-terminal sequence analysis showed the presence of the native protein and several n-terminally degraded components. rl2 and rl3 were n-terminally blocked proteins. after acidic cleavage or cnbr digestio ... | 1998 | 9602056 |
| amp-activated protein kinase inhibits the glucose-activated expression of fatty acid synthase gene in rat hepatocytes. | although it is now clearly established that a number of genes involved in glucose and lipid metabolism are up-regulated by high glucose concentrations in both liver and adipose tissue, the signaling pathway arising from glucose to the transcriptional machinery is still poorly understood. we have analyzed the regulation of fatty acid synthase gene expression by glucose in cultured rat hepatocytes. glucose (25 mm) induces an activation of the transcription of the fatty acid synthase gene, and this ... | 1998 | 9614076 |
| quantitation of absolute pneumocystis carinii nuclear dna content. trophic and cystic forms isolated from infected rat lungs are haploid organisms. | the pneumocystis carinii carinii dna content in nuclei of trophic forms and cysts (spore cases) containing 2, 4, or 8 intracystic bodies, were compared using quantitative fluorescence image analysis. the nuclear dna content was found to be lower than the theoretical limits of feulgen cytophotometry. several fluorescent dna dyes provide brighter staining, but these techniques suffer from nonspecific binding to other cellular components, such as rna. it was demonstrated that the thick glycocalyx s ... | 1998 | 9627984 |
| insulin signaling in the yeast saccharomyces cerevisiae. 2. interaction of human insulin with a putative binding protein. | a putative insulin-binding protein (kd = 0.5 +/- 0.2 microm for human insulin) was partially purified from solubilized plasma membranes of saccharomyces cerevisiaeby wheat germ agglutinin and insulin affinity chromatographies. the binding affinities of various mutant insulin analogues correlated well with their capacities to activate glycogen synthase and snf1 kinase in glucose-induced yeast spheroplasts, the ranking of their relative efficacies in yeast and in isolated rat adipocytes being simi ... | 1998 | 9628731 |
| free radical scavenging activity of fermented papaya preparation and its effect on lipid peroxide level and superoxide dismutase activity in iron-induced epileptic foci of rats. | fermented papaya preparation is a natural health food that has been commercially sold in japan for 2 years. it is made by yeast fermentation of carica papaya linn. we examined the antioxidant action of the fermented papaya preparation on free radicals and lipid peroxidation. free radicals have been related with aging and diseases, such as cancer, diabetes and especially in neurological disorders, for example, parkinson's disease or alzheimer's disease. a diet including variable antioxidant foods ... | 1998 | 9635126 |
| molecular analysis of camnt1p, a mannosyl transferase important for adhesion and virulence of candida albicans. | there is an immediate need for identification of new antifungal targets in opportunistic pathogenic fungi like candida albicans. in the past, efforts have focused on synthesis of chitin and glucan, which confer mechanical strength and rigidity upon the cell wall. this paper describes the molecular analysis of camnt1, a gene involved in synthesis of mannoproteins, the third major class of macromolecule found in the cell wall. camnt1 encodes an alpha-1, 2-mannosyl transferase, which adds the secon ... | 1998 | 9636208 |
| the yeast tribrid system--genetic detection of trans-phosphorylated itam-sh2-interactions. | protein-protein interactions are often dependent on the post-translational modification of one component of a complex. to facilitate the study of these interactions in signal transduction, we have developed the yeast tribrid system, a modification of the yeast two-hybrid system. we demonstrate that the interactions are dependent upon the presence of a tyrosine kinase, an sh2 domain and a tyrosine containing substrate. using the gamma subunit of the high-affinity ige receptor, fc epsilon ri, this ... | 1995 | 9636306 |
| targeting and assembly of the oxoglutarate carrier: general principles for biogenesis of carrier proteins of the mitochondrial inner membrane. | we have studied the targeting and assembly of the 2-oxoglutarate carrier (ogc), an integral inner-membrane protein of mitochondria. the precursor of ogc, synthesized without a cleavable presequence, is transported into mitochondria in an atp- and membrane potential-dependent manner. import of the mammalian ogc occurs efficiently into both mammalian and yeast mitochondria. targeting of ogc reveals a clear dependence on the mitochondrial surface receptor tom70 (the 70 kda subunit of the translocas ... | 1998 | 9639574 |
| prmt 3, a type i protein arginine n-methyltransferase that differs from prmt1 in its oligomerization, subcellular localization, substrate specificity, and regulation. | methylation is one of the many post-translational modifications that modulate protein function. although asymmetric ng,ng-dimethylation of arginine residues in glycine-arginine-rich domains of eucaryotic proteins, catalyzed by type i protein arginine n-methyltransferases (prmt), has been known for some time, members of this enzyme class have only recently been cloned. the first example of this type of enzyme, designated prmt1, cloned because of its ability to interact with the mammalian tis21 im ... | 1998 | 9642256 |
| aut2p and aut7p, two novel microtubule-associated proteins are essential for delivery of autophagic vesicles to the vacuole. | aut2 and aut7, two novel genes essential for autophagocytosis in the yeast saccharomyces cerevisiae were isolated. aut7 was identified as a low copy suppressor of autophagic defects in aut2-1 cells. aut7p is a homologue of the rat microtubule-associated protein (map) light chain 3 (lc3). aut2p and aut7p interact physically. aut7p is attached to microtubules via aut2p, which interacts with tubulins tub1p and tub2p. aut2- and aut7-deleted cells are unable to deliver autophagic vesicles and the pre ... | 1998 | 9649430 |
| different degradation pathways for heterologous glycoproteins in yeast. | rat nerve growth factor receptor ectodomain (ngfre) and escherichia coli beta-lactamase were translocated into the yeast endoplasmic reticulum (er), glycosylated, misfolded and rapidly degraded. ngfre underwent atp-dependent thermosensitive degradation independently of vesicular transport. since no evidence for degradation by the cytoplasmic 26s proteosome complex could be obtained, ngfre appeared to be degraded in the er. beta-lactamase exited the er by vesicular traffic and was transported fro ... | 1998 | 9650582 |
| gtpase activating specificity of rgs12 and binding specificity of an alternatively spliced pdz (psd-95/dlg/zo-1) domain. | regulator of g-protein signaling (rgs) proteins increase the intrinsic guanosine triphosphatase (gtpase) activity of g-protein alpha subunits in vitro, but how specific g-protein-coupled receptor systems are targeted for down-regulation by rgs proteins remains uncharacterized. here, we describe the gtpase specificity of rgs12 and identify four alternatively spliced forms of human rgs12 mrna. two rgs12 isoforms of 6.3 and 5.7 kilobases (kb), encoding both an n-terminal pdz (psd-95/dlg/zo-1) domai ... | 1998 | 9651375 |
| the pleckstrin homology domain of oxysterol-binding protein recognises a determinant specific to golgi membranes. | peripheral membrane proteins are targeted to the cytoplasmic face of specific intracellular membranes. the organelle-specific ligands recognised by peripheral proteins include other proteins and lipids. oxysterol-binding protein (osbp) translocates from the cytoplasm to the golgi apparatus on binding oxygenated derivatives of cholesterol. the mechanism by which osbp recognises the golgi is unknown. it does, however, contain a pleckstrin homology (ph) domain, which in other proteins has been foun ... | 1998 | 9651677 |
| a role for hem2 in cadmium tolerance. | a candida glabrata cadmium-sensitive mutant partially defective in glutathione production and exhibiting a complete absence of phytochelatins was used to clone a gene required for cd tolerance. transformation of the cd-sensitive mutant with a genomic library from the wild-type c. glabrata led to the cloning of a gene that restored cd tolerance and formation of cd-glutathione and cd-phytochelatin complexes. the cloned gene showed high levels of nucleic acid and protein sequence homology to the he ... | 1998 | 9654753 |
| secretory vesicle budding from the trans-golgi network is mediated by phosphatidic acid levels. | phospholipid metabolism plays a central role in regulating vesicular traffic in the secretory pathway. in mammalian cells, activation of a golgi-associated phospholipase d activity by adp-ribosylation factor results in hydrolysis of phosphatidylcholine to phosphatidic acid (pa). this reaction has been proposed to stimulate nascent secretory vesicle budding from the trans-golgi network. it is unclear whether pa itself or diacylglycerol (dag), a metabolite implicated in yeast secretory vesicle for ... | 1998 | 9660750 |
| cain, a novel physiologic protein inhibitor of calcineurin. | calcineurin is a widely distributed protein phosphatase regulated by calcium and calmodulin. it mediates the immunosuppressive actions of drugs such as cyclosporin and fk506, and has been implicated in a number of calcium-sensitive pathways in the nervous system, including regulation of neurotransmitter release and modulation of long-term changes in synaptic plasticity. calcineurin associates physiologically with other proteins, including calmodulin, fkbp12 (fk506-binding protein), the ryanodine ... | 1998 | 9660798 |
| prodrugs of nitroxyl and nitrosobenzene as cascade latentiated inhibitors of aldehyde dehydrogenase. | the prototypic aromatic c-nitroso compound, nitrosobenzene (nb), was shown previously to mimic the effect of nitroxyl (hn=o), the putative active metabolite of cyanamide, in inhibiting aldehyde dehydrogenase (aldh). to minimize the toxicity of nb in vivo, pro-prodrug forms of nb, which were designed to be bioactivated either by an esterase intrinsic to aldh or the mixed function oxidase enzymes of liver microsomes, were prepared. accordingly, the prodrug n-benzenesulfonyl-n-phenylhydroxylamine ( ... | 1998 | 9667978 |
| construction and characterization of a 10-fold genome equivalent rat p1-derived artificial chromosome library. | a rat pac library was constructed in the vector ppac4 from genomic dna isolated from female brown norway rats. this library consists of 215,409 clones arrayed in 614,384-well microtiter plates. an average insert size of 143 kb was estimated from 217 randomly isolated clones, thus representing approximately 10-fold genome coverage. this coverage provides a very high probability that the library contains a unique sequence in genome screening. tests on randomly selected clones demonstrated that the ... | 1998 | 9676425 |
| characterization of kif1c, a new kinesin-like protein involved in vesicle transport from the golgi apparatus to the endoplasmic reticulum. | kinesins comprise a large family of microtubule-based motor proteins, of which individual members mediate specific types of motile processes. using the ezrin domain of the protein-tyrosine phosphatase ptpd1 as a bait in a yeast two-hybrid screen, we identified a new kinesin-like protein, kif1c. kif1c represents a member of the unc104 subfamily of kinesin-like proteins that are involved in the transport of mitochondria or synaptic vesicles in axons. like its homologues, the 1103-amino acid protei ... | 1998 | 9685376 |
| transmembrane neuregulins interact with lim kinase 1, a cytoplasmic protein kinase implicated in development of visuospatial cognition. | the neuregulins are receptor tyrosine kinase ligands that play a critical role in the development of the heart, nervous system, and breast. unlike many extracellular signaling molecules, such as the neurotrophins, most neuregulins are synthesized as transmembrane proteins. to determine the functions of the highly conserved neuregulin cytoplasmic tail, a yeast two-hybrid screen was performed to identify proteins that interact with the 157-amino acid sequence common to the cytoplasmic tails of all ... | 1998 | 9685409 |
| antiphlogistic and antipyretic activity of peucedanum ostruthium. | extracts from the roots of peucedanum ostruthium (l.) koch were investigated for their antiphlogistic and antipyretic effects as well as for their influence on arachidonic acid metabolism. on carrageenan-induced rat paw edema, oral administration of 10% ethanolic extracts led to a marked inhibition of the edema. by means of bioassay-oriented fractionation of extract, 6-(3-carboxybut-2-enyl)-7-hydroxycoumarin proved to be the most important anti-inflammatory principle of the plant. 30 micrograms/ ... | 1998 | 9690339 |
| protective effect of melatonin in a non-septic shock model induced by zymosan in the rat. | in vitro studies have demonstrated that melatonin is a scavenger of oxyradicals and peroxynitrite and an inhibitor of nitric oxide (no) production. recently, it has been proposed that zymosan, a non-bacterial agent, causes inflammation by inducing the production of various cytokines and pro-inflammatory mediators. in the present study we evaluated the effect of melatonin treatment in a non-septic shock model induced by zymosan in the rat. administration of zymosan (500 mg/kg intraperitoneally) i ... | 1998 | 9694401 |
| a novel putative protein-tyrosine phosphatase contains a bro1-like domain and suppresses ha-ras-mediated transformation. | to investigate a potential role of protein-tyrosine phosphatases (ptpases) in myocardial growth and signaling, a degenerate primer-based reverse transcription-polymerase chain reaction approach was used to isolate cdnas for proteins that contain a ptpase catalytic domain. among the 16 cdna clones isolated by reverse transcription-polymerase chain reaction from total neonatal rat cardiomyocyte rna, one, designated ptp-td14, was unique. subsequent isolation and sequencing of a full-length ptp-td14 ... | 1998 | 9694860 |
| a retinoblastoma-binding protein that affects cell-cycle control and confers transforming ability. | the retinoblastoma (rb) gene is one of the most extensively studied tumour-suppressor genes. deletion or inactivation of both rb alleles is an essential, rate-limiting step in the formation of retinoblastoma and osteosarcoma that arise in families that carry mutant rb (ref. 2). rb inactivation is also found in other human tumours. whereas loss of rb function is associated with the loss of cellular proliferative control, introduction of a wild-type rb can suppress cell growth and tumorigenicity. ... | 1998 | 9697699 |
| chiral recognition at the heme active site of nitric oxide synthase is markedly enhanced by l-arginine and 5,6,7,8-tetrahydrobiopterin. | the effects of substrate, l-arg and cofactors, (6r)-l-erythro-5,6,7,8-tetrahydrobiopterin (h4b) and calmodulin (cam), on chiral discrimination by rat neuronal nitric oxide synthase (nnos) for binding the enantiomers of 1-(1-naphthyl)ethylamine (ligand i), 1-cyclohexylethylamine (ligand ii), and 1-(4-pyridyl)ethanol (ligand iii) were studied under anaerobic conditions by optical absorption spectroscopy. the ratio of the dissociation constant (kd) values for the s- and r-enantiomers of ligand i (s ... | 1998 | 9704002 |
| the products of the yeast mms2 and two human homologs (hmms2 and croc-1) define a structurally and functionally conserved ubc-like protein family. | eukaryotic genes encoding ubiquitin-congugating enzyme (ubc)-like proteins have been isolated from both human and yeast cells. the croc-1 gene was isolated by its ability to transactivate c- fos expression in cell culture through a tandem repeat enhancer sequence. the yeast mms2 gene was cloned by its ability to complement the methyl methanesulfonate sensitivity of the mms2-1 mutant and was later shown to be involved in dna post-replication repair. we report here the identification of a human mm ... | 1998 | 9705497 |
| molecular cdna cloning and tissue distribution of mrna encoding a novel atp-binding cassette (abc) half-transporter. | the majority of proteins belonging to the atp-binding cassette (abc) superfamily catalyzes translocation of substrates across biological membranes. employing a reverse transcription-pcr approach with degenerate primers, we have identified a full-length cdna from rat hepatocytes encoding a novel abc transporter termed umat (ubiquitously expressed mammalian abc half-transporter). the deduced sequence of 836 amino acids comprises an n-terminal membrane anchor domain and a single conserved c-termina ... | 1998 | 9705847 |
| the 5'-amp-activated protein kinase inhibits the transcriptional stimulation by glucose in liver cells, acting through the glucose response complex. | 5-amino-4-imidazolecarboxamide riboside (aicar) is known to stimulate rat liver 5'-amp-activated protein kinase (ampk). ampk is the mammalian homologue of snf1p in yeast, involved in derepression of glucose-repressed genes. we used aicar to test if ampk could also play a role in the regulation of glucose-dependent genes in mammalian cells. at a concentration which induces phosphorylation-dependent inactivation of hmg-coa reductase, aicar blocked glucose activation of three glucose responsive gen ... | 1998 | 9708898 |
| identification of a novel ring finger protein as a coregulator in steroid receptor-mediated gene transcription. | using the dna-binding domain of androgen receptor (ar) as a bait in a yeast two-hybrid screening, we have identified a small nuclear ring finger protein, termed snurf, that interacts with ar in a hormone-dependent fashion in both yeast and mammalian cells. physical interaction between ar and snurf was demonstrated by coimmunoprecipitation from cell extracts and by protein-protein affinity chromatography. rat snurf is a highly hydrophilic protein consisting of 194 amino acid residues and comprisi ... | 1998 | 9710597 |
| molecular cloning and expression in saccharomyces cerevisiae of tobacco nadph-cytochrome p450 oxidoreductase cdna. | we obtained information on the full length tobacco nadph-cytochrome p450 oxidoreductase (p450 reductase) by a combination of the cdna clone pctr1 and the genomic dna clone pgtr1. the deduced primary structure consisting of 713 amino acid residues contained sequences corresponding to fmn, fad, and nadph-binding regions. based on this information, we prepared the full-length cdna pftr of tobacco p450 reductase by rt-pcr and expressed it in the yeast saccharomyces cerevisiae. the transformed yeast ... | 1998 | 9720224 |
| subtype- and response element-dependent differences in transactivation by peroxisome proliferator-activated receptors alpha and gamma. | peroxisome proliferator-activated receptors (ppar) modulate transcription by binding to specific peroxisome proliferator-response elements (ppre) through heterodimerization with the 9-cis retinoic acid receptor (rxr). to investigate potential subtype- and response element-dependent differences in transcriptional activation by ppars, we expressed pparalpha or ppargamma2, along with rxralpha, in the yeast saccharoromyces cerevisiae and compared their ability to activate transcription of reporter g ... | 1998 | 9723896 |
| dpm2 regulates biosynthesis of dolichol phosphate-mannose in mammalian cells: correct subcellular localization and stabilization of dpm1, and binding of dolichol phosphate. | biosynthesis of glycosylphosphatidylinositol and n-glycan precursor is dependent upon a mannosyl donor, dolichol phosphate-mannose (dpm). the thy-1negative class e mutant of mouse lymphoma and lec15 mutant chinese hamster ovary (cho) cells are incapable of dpm synthesis. the class e mutant is defective in the dpm1 gene which encodes a mammalian homologue of saccharomyces cerevisiae dpm1p that is a dpm synthase, whereas lec15 is a different mutant, indicating that mammalian dpm1 is not sufficient ... | 1998 | 9724629 |
| molecular cloning and functional characterization of murine sphingosine kinase. | sphingosine-1-phosphate (spp) is a novel lipid messenger that has dual function. intracellularly it regulates proliferation and survival, and extracellularly, it is a ligand for the g protein-coupled receptor edg-1. based on peptide sequences obtained from purified rat kidney sphingosine kinase, the enzyme that regulates spp levels, we report here the cloning, identification, and characterization of the first mammalian sphingosine kinases (murine sphk1a and sphk1b). sequence analysis indicates t ... | 1998 | 9726979 |
| binding of rat brain hexokinase to recombinant yeast mitochondria: effect of environmental factors and the source of porin. | heterologous binding of rat brain hexokinase to wild type, porinless, and recombinant yeast mitochondria expressing human porin was assessed, partially characterized, and compared to that in the homologous system (rat liver mitochondria). with porin-containing yeast mitochondria it is shown that (i) a significant, saturable association occurs; (ii) its extent and apparent affinity, correlated with the origin of porin, are enhanced in the presence of dextran; (iii) the binding requires mg ions an ... | 1998 | 9733091 |
| the sequence, bacterial expression, and functional reconstitution of the rat mitochondrial dicarboxylate transporter cloned via distant homologs in yeast and caenorhabditis elegans. | the dicarboxylate carrier (dic) belongs to a family of transport proteins found in the inner mitochondrial membranes. the biochemical properties of the mammalian protein have been characterized, but the protein is not abundant. it is difficult to purify and had not been sequenced. we have used the sequence of the distantly related yeast dic to identify a related protein encoded in the genome of caenorhabditis elegans. then, related murine expressed sequence tags were identified with the worm seq ... | 1998 | 9733776 |
| identification of a novel cortactin sh3 domain-binding protein and its localization to growth cones of cultured neurons. | cortactin is an actin-binding protein that contains several potential signaling motifs including a src homology 3 (sh3) domain at the distal c terminus. translocation of cortactin to specific cortical actin structures and hyperphosphorylation of cortactin on tyrosine have been associated with the cortical cytoskeleton reorganization induced by a variety of cellular stimuli. the function of cortactin in these processes is largely unknown in part due to the lack of information about cellular bindi ... | 1998 | 9742101 |
| using yeast to study glucocorticoid receptor phosphorylation. | the glucocorticoid receptor (gr) is a phosphoprotein and a member of the steroid/thyroid receptor superfamily of ligand dependent transcription factors. when the glucocorticoid receptor is expressed in yeast (saccharomyces cerevisiae), it is competent for signal transduction and transcriptional regulation. we have studied the glucocorticoid receptor phosphorylation in yeast and demonstrated that the receptor is phosphorylated in both the absence and presence of hormone, on serine and threonine r ... | 1998 | 9749836 |
| brewer's yeast and saccharomyces boulardii both attenuate clostridium difficile-induced colonic secretion in the rat. | saccharomyces boulardii (sb), a nonpathogenic yeast, has been used to prevent recurrences of clostridium difficile (c.diff) -associated diarrhea. a single report suggested that treatment with saccharomyces cerevisiae (sc), commonly called brewer's yeast (by), facilitates treatment of persistent c.diff infection. we conducted this experiment to determine whether c.diff toxin a-induced colonic secretion in the rat is blunted by pretreatment with either sb or by. we employed closed cecal pouches in ... | 1998 | 9753273 |
| identification and characterization in xenopus of xsmggds, a ralb binding protein. | we have previously isolated a cdna coding for a ralb protein from a xenopus maternal library. by mutagenesis of ralb and microinjection of its mrna in embryos we show that ralb is involved in xenopus early development. we have used the yeast two-hybrid system to screen a xenopus maternal cdna library in order to isolate proteins interacting to ralb. we have identified a full-length cdna encoding a protein homologous to mammalian smggds. the xenopus gene shows 84.6% identity with the mammalian co ... | 1998 | 9753634 |
| begain (brain-enriched guanylate kinase-associated protein), a novel neuronal psd-95/sap90-binding protein. | psd-95/sap90 is a synaptic membrane-associated guanylate kinase with three pdz, one sh3, and one guanylate kinase (gk) domain. psd-95/sap90 binds various proteins through the pdz domains and organizes synaptic junctions. psd-95/sap90 also interacts with the postsynaptic density (psd) fraction-enriched protein, named sapap (also called gkap and dap), through the gk domain. sapap is triton x-100-insoluble and recruits psd-95/sap90 into the triton x-100-insoluble fraction in the transfected cells, ... | 1998 | 9756850 |
| molecular and pharmacological characterization of recombinant rat/mice n-methyl-d-aspartate receptor subtypes in the yeast saccharomyces cerevisiae. | the genes encoding the ionotropic n-methyl-d-aspartate (nmda) receptor subunits nr1a, nr2b and nr2d were cloned in the multi-copy yeast-escherichia coli shuttle vectors pmbo1 and pmb02. the protease-deficient yeast saccharomyces cerevisiae c13-abys-86 (leu-, ura-, his-) was transformed with the recombinant plasmids pmbnr1a (leu+), pmbnr1a/pmbnr2d (ura+), pmbnr1a/pmbnr2d/ pmbnr2b (his+) or pmbnr1a/pmbnr2a/pmbnr2b, respectively, and was used to express the different nmda receptor subunit genes. we ... | 1998 | 9760184 |
| colorimetric determination of succinic acid using yeast succinate dehydrogenase. | an enzymatic method for the rapid determination of succinic acid in biological fluids was developed utilizing yeast mitochondria as a source of succinate dehydrogenase. the yeast enzyme catalyzes a complete stoichiometric reduction of 2- (p-iodophenyl)-3-(p-nitrophenyl)-5-tetrazolium chloride to a red formazan. the formazan is extracted into ethylacetate and its absorbance measured at 490 nm. the method is simple, specific, reproducible, and very sensitive (0.01 to 0.14 mumol). the yeast enzyme ... | 1978 | 9762145 |
| trematode and monogenean rrna its2 secondary structures support a four-domain model. | the secondary structure of rrna internal transcribed spacer 2 is important in the process of ribosomal biogenesis. trematode its sequences are poorly conserved and difficult to align for phylogenetic comparisons above a family level. if a conserved secondary structure can be identified, it can be used to guide primary sequence alignments. its2 sequences from 39 species were compared. these species span four orders of trematodes (echinostomiformes, plagiorchiformes, strigeiformes, and paramphisto ... | 1998 | 9767686 |
| a novel phosphatase regulating neurite extension on cns inhibitors. | the inability of injured axons to regenerate in the adult mammalian central nervous system is thought to be in part due to inhibitory molecules synthesized by oligodendrocytes and present in myelin. we describe the cloning of a cdna encoding a novel neuronal protein, named nerpp-2c, which is distantly related to protein phosphatase 2c and plays a role in the inhibitory response pathway to myelin inhibitors. nerpp-2c is expressed in neuronal cell lines and in rat brain. expression in rat is detec ... | 1998 | 9770338 |
| novel mutants of nab corepressors enhance activation by egr transactivators. | the ngfi-a binding corepressors nab1 and nab2 interact with a conserved domain (r1 domain) within the egr1/ngfi-a and egr2/krox20 transactivators, and repress the transcription of egr target promoters. using a novel adaptation of the yeast two-hybrid screen, we have identified several point mutations in nab corepressors that interfere with their ability to bind to the egr1 r1 domain. surprisingly, nab proteins bearing some of these mutations increased egr1 activity dramatically. the mechanism un ... | 1998 | 9774344 |
| role of hepatic fatty acid:coenzyme a ligases in the metabolism of xenobiotic carboxylic acids. | 1. formation of acyl-coenzymes (co)a occurs as an obligatory step in the metabolism of a variety of endogenous substrates, including fatty acids. the reaction is catalysed by atp-dependent acid:coa ligases (ec 6.2.1.1-2.1.3; amp forming), classified on the basis of their ability to conjugate saturated fatty acids of differing chain lengths, short (c2-c4), medium (c4-c12) and long (c10-c22). the enzymes are located in various cell compartments (cytosol, smooth endoplasmic reticulum, mitochondria ... | 1998 | 9784915 |
| identification of a novel highly conserved gene in the centromeric part of the major histocompatibility complex. | a novel highly conserved gene designated sacm2l alias are1) has been identified and fine mapped in the centromeric part of the major histocompatibility complex in rat, human, and mouse. sacm2l is closely linked to the ribosomal protein s18 gene rps18 with a distance of about 450 bp between the respective translational start points. numerous sacm2l-homologous est sequences can be identified in the database. northern blot experiments of rat sacm2l revealed a transcript of 3 kb in each organ tested ... | 1998 | 9790748 |
| binding of myosin essential light chain to the cytoskeleton-associated protein iqgap1. | the 190 kd human iqgap1 protein, by virtue of its n-terminal calponin-homology domain, is found associated with the actin cytoskeleton, and is capable of cross-linking actin filaments. iqgap1 complexes with several proteins, including the rho family gtpases cdc42 and rac, as well as calmodulin. it was previously noted that one of the iq motifs of iqgap1 displays significant similarity to a myosin heavy chain iq motif responsible for binding the calmodulin-related myosin essential light chain (el ... | 1998 | 9790945 |
| the use of the yeast two hybrid system to evaluate erbb-3 interactions with sh2 domain containing proteins. | several mutations in the tyrosine kinase domain of erbb-3 have been postulated to render this enzyme catalytically inactive. to test which amino acid mutations in erbb-3 might be critical for kinase inactivation, we used a yeast two hybrid assay of protein-protein interaction. we monitored restoration of erbb-3 kinase activity by investigating the ability of wild type or mutant erbb-3 to associate with the sh2 containing proteins syp and phosphatidyl-inositol-3-kinase (pi3k). our results demonst ... | 1998 | 9791008 |
| valine 904, tyrosine 898, and cysteine 908 in na,k-atpase alpha subunits are important for assembly with beta subunits. | a 26-amino acid sequence in an extracellular loop of the na,k-atpase alpha subunit between membrane-spanning segments 7 and 8 has been shown to bind to the beta subunit of na,k-atpase and to promote alphabeta assembly (lemas, m. v., hamrick, m., takeyasu, k., and fambrough, d. m. (1994) j. biol. chem. 269, 8255-8259) when this 26-amino acid sequence of the rat na,k-atpase alpha3 subunit was replaced by the corresponding sequence of the rat gastric h,k-atpase alpha subunit, the chimeric alpha sub ... | 1998 | 9792642 |
| association of hap1 isoforms with a unique cytoplasmic structure. | hap1 is a neural protein and interacts with the huntington's disease protein huntingtin. there are at least two hap1 isoforms, hap1-a and hap1-b, which have different c-terminal amino acid sequences. here we report that both hap1 isoforms associate with a unique cytoplasmic structure in neurons of rat brain. the hap1-immunoreactive structure appears as an inclusion that is an oval mass of electron-dense material, 0.5-3 microm in diameter, containing many curvilinear or ring-shaped segments, and ... | 1998 | 9798945 |
| metabolism of benzo[a]pyrene and benzo[a]pyrene-7,8-diol by human cytochrome p450 1b1. | benzo[a]pyrene (b[a]p), a ubiquitous environmental, tobacco and dietary carcinogen, has been implicated in human cancer etiology. the role of human cytochrome p450 1b1 in the metabolism of b[a]p is poorly understood. using microsomal preparations of human p450 1a1, 1a2 and 1b1 expressed in baculovirus-infected insect cells, as well as human and rat p450 1b1 expressed in yeast, we have determined the metabolism of b[a]p, with and without the addition of exogenous epoxide hydrolase, and b[a]p-7,8- ... | 1998 | 9806168 |
| recently identified molecular aspects of intestinal iron absorption. | gene mapping techniques to identify gene mutations in humans and animals with phenotypic abnormalities in iron metabolism are providing important insights into the probable molecular mediators of intestinal iron absorption. positional gene cloning in humans with hereditary hemochromatosis has identified a mutation in a novel major histocompatibility complex (mhc) gene called hfe that is likely to be involved in regulating intestinal iron absorption. in addition, recent observations based on posi ... | 1998 | 9808632 |
| binding of dynein light chain (pin) to neuronal nitric oxide synthase in the absence of inhibition. | pin, an 89-amino-acid polypeptide found in a rat hippocampal cdna library using the yeast two-hybrid system and various neuronal nitric oxide synthase (nnos) fragments as bait, was reported to be an inhibitor of nnos (science 274, 774-778, 1996). pin reportedly inhibited nnos selectively and did not interact with either the endothelial or inducible nitric oxide synthase isoforms. inhibition was attributed to the ability of pin to dissociate the catalytically active nnos homodimer. pin is a dynei ... | 1998 | 9808772 |
| hpttg, a human homologue of rat pttg, is overexpressed in hematopoietic neoplasms. evidence for a transcriptional activation function of hpttg. | we have isolated a human cdna clone encoding a novel protein of 22 kda that is a human counterpart of the rat oncoprotein pttg. we show that the corresponding gene (hpttg) is overexpressed in jurkat cells (a human t lymphoma cell line) and in samples from patients with different kinds of hematopoietic malignancies. analysis of the sequence showed that hpttg has an amino-terminal basic domain and a carboxyl-terminal acidic domain, and that it is a proline-rich protein with several putative sh3-bi ... | 1998 | 9811450 |
| human carbon catabolite repressor protein (ccr4)-associative factor 1: cloning, expression and characterization of its interaction with the b-cell translocation protein btg1. | the human btg1 protein is thought to be a potential tumour suppressor because its overexpression inhibits nih 3t3 cell proliferation. however, little is known about how btg1 exerts its anti-proliferative activity. in this study, we used the yeast 'two-hybrid' system to screen for interacting protein partners and identified human carbon catabolite repressor protein (ccr4)-associative factor 1 (hcaf-1), a homologue of mouse caf-1 (mcaf-1) and saccharomyces cerevisiae ycaf-1/pop2. in vitro the hcaf ... | 1998 | 9820826 |
| expression of active human tissue-type plasminogen activator in escherichia coli. | the formation of native disulfide bonds in complex eukaryotic proteins expressed in escherichia coli is extremely inefficient. tissue plasminogen activator (tpa) is a very important thrombolytic agent with 17 disulfides, and despite numerous attempts, its expression in an active form in bacteria has not been reported. to achieve the production of active tpa in e. coli, we have investigated the effect of cooverexpressing native (dsba and dsbc) or heterologous (rat and yeast protein disulfide isom ... | 1998 | 9835579 |
| cloning of a third member of the d52 gene family indicates alternative coding sequence usage in d52-like transcripts. | d52 proteins are emerging as signalling molecules which may be regulators of cell proliferation. having previously reported the existence of the human d52 gene family, comprising the hd52 and hd53 genes expressed in human breast carcinoma, we report the identification of a novel human gene hd54 (tpd52l2), which represents a third d52 gene family member. in situ mapping placed the hd54 gene on human chromosome 20q13.2-q13.3, a localization distinct from those of both hd52 and hd53 genes. the iden ... | 1998 | 9838088 |
| enhanced clearance of a multiple antibiotic resistant staphylococcus aureus in rats treated with pgg-glucan is associated with increased leukocyte counts and increased neutrophil oxidative burst activity. | pgg-glucan [betafectin], a highly purified soluble beta-(1-6)-branched beta-(1 3)-linked glucan isolated from saccharomyces cerevisiae, has broad in vitro and in vivo anti-infective activities unrelated to cytokine induction. here we present in vivo results on the anti-infective activity of pgg-glucan against a multiple antibiotic resistant staphylococcus aureus. pgg-glucan (0.25-4 mg/kg) was administered intramuscularly to male wistar rats 48 h, 24 h, and 4 h before and 4 h after intraperitonea ... | 1998 | 9848393 |
| cloning and characterization of a novel mammalian pp2c isozyme. | pp2c is a structurally diversified protein phosphatase family with a wide range of functions in cellular signal transduction. a novel pp2c subtype, designated pp2cdelta, was identified from a rat cdna clone, which encodes a protein of 392 amino acid residues. while pp2cdelta shares approximately 30% sequence identity in its catalytic domain with the mammalian pp2c, it lacks a 90-residue carboxyl-terminal sequence conserved in mammalian pp2c. northern blot analysis showed that pp2cdelta is widely ... | 1998 | 9857069 |
| identification and characterization of stk12/aik2: a human gene related to aurora of drosophila and yeast ipl1. | mutations in aurora of drosophila and related saccharomyces cerevisiae ipl1 protein kinases are known to cause abnormal chromosome segregation. we earlier isolated a cdna encoding a novel human protein kinase aik which shares high amino acid identity with the aurora/ipl1 protein kinase family. in the present study, a second human cdna highly homologous to aurora/ipl1 (aik2) was identified and the nucleotide sequence was determined (gene symbol stk12). the c-terminal kinase domain of the stk12 en ... | 1998 | 9858806 |
| mint 3: a ubiquitous mint isoform that does not bind to munc18-1 or -2. | mint 1 and 2 are proteins that bind to munc18-1, an essential component of the synaptic vesicle fusion machinery, and are detectably expressed only in neurons [okamoto and südhof, j. biol. chem. 272, 31459-31464 (1997)]. mint 1 and 2 are composed of a variable n-terminal region that includes a conserved munc18-1-binding site, and a constant c-terminal region that contains one ptb and two pdz domains. we have now identified a third mint isoform, mint 3. similar to mint 1 and 2, the c-terminal hal ... | 1998 | 9860131 |
| the hsp70 gene family in pneumocystis carinii: molecular and phylogenetic characterization of cytoplasmic members. | pneumocystis carinii, a major opportunistic lung pathogen of aids patients, is found in a number of mammals and is proposed to be a member of the fungi. in this work, several members of the highly conserved hsp70 multigene family were characterized from rat-derived p. carinii. previously, we reported characterization of the er resident hsp70 homolog known as bip from prototype (p.c. carinii) and variant (p. c. rattus) strains of the organism. we report here, from p. c. carinii, characterization ... | 1998 | 9864849 |
| proteins involved in membrane transport between the er and the golgi apparatus: 21 putative plant homologues revealed by dbest searching. | numerous proteins have been identified in yeast and mammalian cells which are involved in trafficking between the endoplasmic reticulum and the golgi apparatus. a great number of partial cdna sequences now available from the two major plant model species, arabidopsis thaliana and oryza sativa, makes it possible to identify putative plant homologues of known genes/proteins from non-plant species. the authors used this approach to screen the database of expressed sequence tags (dbest) in order to ... | 1998 | 9878102 |
| characterization of the mouse src homology 3 domain gene sh3d2c on chr 7 demonstrates coexpression with huntingtin in the brain and identifies the processed pseudogene sh3d2c-ps1 on chr 2. | formation of intracellular protein complexes is often mediated by src homology 3 domain-containing proteins interacting with proline-rich target sequences on other proteins. the sh3d2c gene or its rat/human orthologs have been implicated in synaptic vesicle recycling due to interaction with dynamin i and synaptojanin in nerve terminals. in a yeast two-hybrid system, association with a huntingtin fragment containing an elongated stretch of polyglutamines was observed recently. by genetic mapping ... | 1998 | 9878254 |
| intermolecular and interdomain interactions of a dynamin-related gtp-binding protein, dnm1p/vps1p-like protein. | dnm1p/vps1p-like protein (dvlp) is a mammalian member of the dynamin gtpase family, which is classified into subfamilies on the basis of the structural similarity. mammalian dynamins constitute the dynamin subfamily. dvlp belongs to the vps1 subfamily, which also includes yeast vps1p and dnm1p. typical structural features that discriminate between members of the vps1 and dynamin subfamilies are that the former lacks the pleckstrin homology and pro-rich domains. dynamin exists as tetramers under ... | 1999 | 9915810 |
| cloning and characterization of prax-1. a new protein that specifically interacts with the peripheral benzodiazepine receptor. | using a cytoplasmic domain of the peripheral benzodiazepine receptor (pbr) as a bait in the yeast two-hybrid system, we have isolated a cdna encoding a new protein that specifically interacts with pbr. we named it prax-1, for peripheral benzodiazepine receptor-associated protein 1. prax-1 is a 1857-amino acid protein, the sequence of which was structurally unrelated to any known proteins. the gene encoding prax-1 is located in the q22-q23 region of the long arm of the human chromosome 17. the pr ... | 1999 | 9915832 |
| atp inhibition of katp channels: control of nucleotide sensitivity by the n-terminal domain of the kir6.2 subunit. | 1. to gain insight into the role of the cytoplasmic regions of the kir6.2 subunit in regulating channel activity, we have expressed the sulphonylurea receptor sur1 with kir6.2 subunits containing systematic truncations of the n- and c-termini. up to 30 amino acids could be truncated from the n-terminus, and up to 36 amino acids from the c-terminus without loss of functional channels in co-expression with sur1. furthermore, kir6.2deltac25 and kir6. 2deltac36 subunits expressed functional channels ... | 1999 | 9925874 |
| direct interaction of microtubule- and actin-based transport motors. | the microtubule network is thought to be used for long-range transport of cellular components in animal cells whereas the actin network is proposed to be used for short-range transport, although the mechanism(s) by which this transport is coordinated is poorly understood. for example, in sea urchins long-range ca2+-regulated transport of exocytotic vesicles requires a microtubule-based motor, whereas an actin-based motor is used for short-range transport. in neurons, microtubule-based kinesin mo ... | 1999 | 9930703 |
| a transforming mutation enhances the activity of the c-kit soluble tyrosine kinase domain. | an activating mutation (dy814) located in the catalytic domain of the c-kit receptor has been found in mastocytomas from human, mouse and rat. we evaluated the enzymic properties of purified wild-type (wt) and dy814 tyrosine kinase domains expressed in pichia pastoris. a linker encoding the flag epitope was fused to c-kit cdna species, enabling affinity purification of the proteins with anti-flag antibodies. yeast lysates expressing dy814 contained multiple tyrosine-phosphorylated proteins, wher ... | 1999 | 9931308 |
| identification of ce-af-6, a novel caenorhabditis elegans protein, as a putative ras effector. | mammalian ras proteins associate with multiple effectors, including raf, ral guanine nucleotide dissociation stimulator, phosphoinositide 3-kinase and af-6. in the nematode caenorhabditis elegans, lin-45/raf has been identified genetically as an effector of let-60/ras. to search for other effectors in c. elegans, we carried out a yeast two-hybrid screening for let-60-associating proteins. the screening identified a novel protein, designated ce-af-6, which exhibited a strong structural homology w ... | 1998 | 9931431 |
| the nuclear cap-binding complex is a novel target of growth factor receptor-coupled signal transduction. | in an attempt to further understand how nuclear events (such as gene expression, nuclear import/export, and cell cycle checkpoint control) might be subject to regulation by extracellular stimuli, we sought to identify nuclear activities under growth factor control. using a sensitive photoaffinity labeling assay that measured [alpha-32p]gtp incorporation into nuclear proteins, we identified the 20-kda subunit of the nuclear cap-binding complex (cbc) as a protein whose binding activity is greatly ... | 1999 | 9933612 |
| mammalian homologue of the caenorhabditis elegans unc-76 protein involved in axonal outgrowth is a protein kinase c zeta-interacting protein. | by the yeast two-hybrid screening of a rat brain cdna library with the regulatory domain of protein kinase c zeta (pkczeta) as a bait, we have cloned a gene coding for a novel pkczeta-interacting protein homologous to the caenorhabditis elegans unc-76 protein involved in axonal outgrowth and fasciculation. the protein designated fez1 (fasciculation and elongation protein zeta-1) consisting of 393 amino acid residues shows a high asp/glu content and contains several regions predicted to form amph ... | 1999 | 9971736 |
| phosphatidylinositol synthesis in mycobacteria. | the metabolism and synthesis of an important mycobacterial lipid component, phosphatidylinositol (pi), and its metabolites, was studied in mycobacterium smegmatis and m. smegmatis subcellular fractions. little is known about the synthesis of pi in prokaryotic cells. only a cell wall fraction (p60) in m. smegmatis was shown to possess pi synthase activity. product was identified as pi by migration on tlc, treatment with phospholipase c and ion exchange chromatography. pi was the only major produc ... | 1999 | 9989274 |
| peripheral blood neutrophils of germ-free rats modified by in vivo granulocyte-colony-stimulating factor and exposure to natural environment. | peripheral blood neutrophils from germ-free (gf), specific-pathogen-free (spf) and conventional (cv) rats were compared. besides neutropenia and impaired superoxide anion generation as previously reported, it was found that gf rats had lower phagocytic function (70%) and generated less nitric oxide than the other rats. gf and spf rats were injected with recombinant human granulocyte-colony-stimulating factor (rhg-csf), or were transferred to natural environment. it was found that total number, p ... | 1999 | 10023860 |
| synergistic activation of yeast-expressed rat androgen receptor by modulators of protein kinase-a. | we have employed a yeast (saccharomyces cerevisiae) based rat androgen receptor expression system to examine the cross-talk between different signalling pathways. we report here the synergistic modulation of androgen regulated transcriptional activation of beta-galactosidase reporter activity by the activators of protein kinase-a, like forskolin and 8-bromo-cyclic amp. a similar ligand-dependent enhancement of reporter activity compared to a dht treated control has been noticed with okadaic acid ... | 1999 | 10024442 |
| identification of a novel domain shared by putative components of the endocytic and cytoskeletal machinery. | we have identified a approximately 140 amino acid domain that is shared by a variety of proteins in budding and fission yeast, nematode, rat, mouse, frog, oat, and man. typically, this domain is located within 20 residues of the n-terminus of the various proteins. the percent identity among the domains in the 12 proteins ranges from 42 to 93%, with 16 absolutely conserved residues: n-x(11-13)-v-x2-a-t-x(34-36)-r-x(7-8)-w-r-x3-k-x12-g-x-e-x15 -l-x11-12-d-x-g-r-x11-d-x7-r. even though these protei ... | 1999 | 10048338 |
| molecular cloning and functional characterization of rat delta-6 fatty acid desaturase. | mammalian cdna fragments putatively encoding amino acid sequences characteristic of the fatty acid desaturase were obtained using expressed sequence tag (est) sequence informations. these fragments were subsequently used to screen a rat liver cdna library, yielding a 1573-bp clone. expression of dna fragment containing either of two possible open reading frames (nucleotide numbers 97-1431 and 148-1431) of the isolated clone in yeast led to the accumulation of gamma-linolenic acid in the presence ... | 1999 | 10049752 |
| a schizosaccharomyces pombe gene, ksg1, that shows structural homology to the human phosphoinositide-dependent protein kinase pdk1, is essential for growth, mating and sporulation. | fission yeast (schizosaccharomyces pombe) requires inositol for growth, mating and sporulation. to define putative genes that are involved in the processing and transduction of the inositol signal, mutants that are temperature sensitive for growth and sporulation were selected on a medium containing non-limiting amounts of inositol. two such mutants (ksg1-208 and ksg1-358) were analyzed, which are impaired in mating and sporulation at 30 degrees c and undergo growth arrest in the g2 phase of the ... | 1999 | 10071224 |
| opposite behaviors of reactive metabolites of tienilic acid and its isomer toward liver proteins: use of specific anti-tienilic acid-protein adduct antibodies and the possible relationship with different hepatotoxic effects of the two compounds. | tienilic acid (ta) is responsible for an immune-mediated drug-induced hepatitis in humans, while its isomer (tai) triggers a direct hepatitis in rats. in this study, we describe an immunological approach developed for studying the specificity of the covalent binding of these two compounds. for this purpose, two different coupling strategies were used to obtain ta-carrier protein conjugates. in the first strategy, the drug was linked through its carboxylic acid function to amine residues of carri ... | 1999 | 10077492 |
| notch signaling imposes two distinct blocks in the differentiation of c2c12 myoblasts. | notch signal transduction regulates expression of downstream genes through the activation of the dna-binding protein su(h)/cbf1. in drosophila most of notch signaling requires su(h); however, some notch-dependent processes occur in the absence of su(h) suggesting that notch signaling does not always involve activation of this factor. using constitutively active forms of notch lacking cbf1-interacting sequences we identified a notch signaling pathway that inhibits myogenic differentiation of c2c1 ... | 1999 | 10079231 |
| molecular cloning of clc chloride channels in oreochromis mossambicus and their functional complementation of yeast clc gene mutant. | we have cloned two members of the clc chloride channel family (omclc-3 and omclc-5) from gill cdna libraries of the euryhaline tilapia oreochromis mosammbicus. at the amino acid level, omclc-3 is 90.5% identical to rat clc-3 and omclc-5 is 79.2% identical to rat clc-5. ribonuclease protection assay revealed that omclc-5 was mainly expressed in the gill, kidney, and intestine in both freshwater- (fw) and seawater- (sw) adapted tilapia. although the mrna of omclc-3 was broadly expressed in tissues ... | 1999 | 10082675 |
| a conserved biogenesis pathway for nucleoporins: proteolytic processing of a 186-kilodalton precursor generates nup98 and the novel nucleoporin, nup96. | the mammalian nuclear pore complex (npc) is comprised of approximately 50 unique proteins, collectively known as nucleoporins. through fractionation of rat liver nuclei, we have isolated >30 potentially novel nucleoporins and have begun a systematic characterization of these proteins. here, we present the characterization of nup96, a novel nucleoporin with a predicted molecular mass of 96 kd. nup96 is generated through an unusual biogenesis pathway that involves synthesis of a 186-kd precursor p ... | 1999 | 10087256 |
| preparation of yac end fragments from the whitehead/mit mouse yac library prml vectors. | 1999 | 10090972 | |
| delta 9-fatty acid desaturase from arachidonic acid-producing fungus. unique gene sequence and its heterologous expression in a fungus, aspergillus. | based on the sequence information for delta 9-desaturase genes (from rat, mouse and yeast), which are involved in the desaturation of palmitic acid and stearic acid to palmitoleic acid and oleic acid, respectively, the corresponding cdna and genomic gene were cloned from the fungal strain, mortierella alpina 1s-4, which industrially produces arachidonic acid. there was a cytochrome b5-like domain linked to the carboxyl terminus of this mortierella desaturase, as also seen in the yeast delta 9-de ... | 1999 | 10091601 |