Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| antibacterial activity of gentamicin and ciprofloxacin against gram-negative bacteria: interactions with pig and calf sera. | the antibacterial activity of pig and calf serum and its ability to interact with gentamicin and ciprofloxacin were studied in vitro using escherichia coli k-12, proteus rettgeri (sanelli) and klebsiella pneumoniae atcc 10031. the antimicrobial activity of the above drugs, alone or in combination with serum, was investigated by the checkerboard method and expressed as the minimal inhibitory concentration (microg ml-1). pig serum (25%) with gentamicin had a synergistic antibacterial effect agains ... | 1999 | 10208763 |
| crystallization and preliminary x-ray diffraction analysis of a biologically active fragment of cd55. | crystals have been grown of two of the domains of cd55. this is the first report of crystallization of a short consensus repeat (scr) domain containing protein. cd55 is a widely expressed polymorphic glycoprotein, which functions as a complement regulator by inhibiting assembly and promoting destruction of c3 and c5 convertases. as a key regulator of complement, cd55 is implicated in the hyperacute rejection of xenografts from pigs into primates. it is also commonly hijacked as a receptor by vir ... | 1999 | 10329784 |
| the use of non-immune plasma powder in the prophylaxis of neonatal escherichia coli diarrhoea in calves. | the protective use of plasma powder from cattle and swine against experimentally induced neonatal e. coli diarrhoea in colostrum-deprived calves was examined. diarrhoea was induced with a strain expressing f5+ fimbriae and a strain expressing f17+ fimbriae. in all groups supplemented with bovine plasma powder, diarrhoea and fever were less severe than in the control groups. for the groups infected with the f5+ e. coli strain, a reduction in excretion of the challenge strain by 2-4 orders of magn ... | 1999 | 10337234 |
| effects of erdosteine and its metabolites on bacterial adhesiveness. | erdosteine (cas 84611-23-4) is administered as a mucolytic drug in patients with pulmonary disorders who suffer from a thickening of bronchial mucus with altered physico-chemical characteristics. erdosteine itself does not have a free thiol group but its metabolization produces active metabolites with a -sh group that is capable of breaking disulfide bonds of mucins and improving the mucociliary clearance of the airways, and thus reproducing the effects of the class of muco-active drugs having a ... | 1999 | 10337454 |
| multiple antibiotic resistance patterns of escherichia coli isolates from swine farms. | antibiotic resistance of escherichia coli from sows and pigs was determined to compare patterns between pigs of various ages and degrees of antibiotic use. resistance patterns differed between farm types and pigs of differing ages, indicating that pig age and degree of antibiotic use affect resistance of fecal e. coli. | 1999 | 10347077 |
| identification of the rna-binding, dimerization, and eif4gi-binding domains of rotavirus nonstructural protein nsp3. | the rotavirus nonstructural protein nsp3 is a sequence-specific rna binding protein that binds the nonpolyadenylated 3' end of the rotavirus mrnas. nsp3 also interacts with the translation initiation factor eif4gi and competes with the poly(a) binding protein. deletion mutations and point mutations of nsp3 from group a rotavirus (nsp3a), expressed in escherichia coli, indicate that the rna binding domain lies between amino acids 4 and 149. similar results were obtained with nsp3 from group c rot ... | 1999 | 10364288 |
| phosphate release during microtubule assembly: what stabilizes growing microtubules? | the molecular mechanism underlying microtubule dynamic instability depends on the relationship between the addition of tubulin-gtp to a growing microtubule and its hydrolysis in the microtubule lattice to tubulin-gdp, with release of inorganic phosphate (pi). since this relationship remains controversial, we have re-examined the release of pi upon microtubule assembly using a fluorometric assay for pi, based on the phosphate-binding protein of escherichia coli [brune m., hunter, j. l., corrie, j ... | 1999 | 10387063 |
| the use of polymerase chain reaction for determination of virulence factors of escherichia coli strains isolated from pigs in poland. | e. coli strains isolated from pigs with postweaning diarrhea or edema disease were tested by phenotypic and genotypic methods for the presence of virulence antigens and genes, respectively. the slide agglutination and elisa analyses were used for determination of f4, f5, f6, f17, and f41 fimbriae whereas the prevalence of fimbrial feda and toxin elti, esti, estii, stx1, stx2 and stx2e genes were recorded by the means of pcr. only f4 antigen (ac variant) was found in strains of the serogroup o149 ... | 1999 | 10391503 |
| gram positive bacteria induce il-6 and il-8 production in human alveolar macrophages and epithelial cells. | inhalation of dust from swine confinement buildings results in an acute inflammatory reaction in the respiratory tract. the dust has a high microbial content, dominated by gram positive bacteria. the aim of the present study was to evaluate the significance of bacteria in the induction of il-6 and il-8 release from respiratory epithelial cells and alveolar macrophages. the results would give an indication to what extent the bacteria contribute to the toxic inflammation following exposure to swin ... | 1999 | 10392756 |
| expression and immunogenicity of a mutant diphtheria toxin molecule, crm(197), and its fragments in salmonella typhi vaccine strain cvd 908-htra. | mutant diphtheria toxin molecule crm(197) and fragments thereof were expressed in attenuated salmonella typhi cvd 908-htra, and the constructs were tested for their ability to induce serum antitoxin. initially, expressed proteins were insoluble, and the constructs failed to induce neutralizing antitoxin. soluble crm(197) was expressed at low levels by utilizing the hemolysin a secretion system from escherichia coli. | 1999 | 10417208 |
| recovery and survival of escherichia coli o157:h7 in reconditioned pork-processing wastewater. | the pathogen escherichia coli o157:h7 has been recovered from various water sources and food samples. the growth potential of this bacterium in nutrient-limited, reconditioned wastewater from a pork-processing plant was determined over a temperature range of 4 to 46 degrees c. even though the biological oxygen demand of the wastewater was <2 mg/liter, results of bioassays for assimilable organic carbon and the coliform growth response of the water suggested that sufficient nutrients were present ... | 1999 | 10419263 |
| an inhibitor of angiotensin converting enzyme (enalapril) augments endotoxin-induced hypotension in the pig. | septic shock causes an extensive inflammatory reaction including increased capillary leakage and a decrease in systemic blood pressure. human septic shock can be replicated in the endotoxaemic pig. angiotensin converting enzyme (ace) is involved in the degradation of bradykinin, an inflammatory mediator, and in the regulation of blood pressure. inhibition of ace is a common approach to reduce hypertension as well as left ventricular insufficiency. fifteen anaesthetised pigs received a continuous ... | 1999 | 10422218 |
| adjunctive oral corticosteroids reduce renal scarring: the piglet model of reflux and acute experimental pyelonephritis. | we investigate the efficacy of antibiotics combined with corticosteroid in diminishing post-pyelonephritic renal scarring compared to standard antibiotic therapy. | 1999 | 10458387 |
| f165(1) fimbriae of the p fimbrial family inhibit the oxidative response of porcine neutrophils. | the f165(1) fimbrial system has been associated with the resistance of escherichia coli o115:k"v165" to phagocytic killing by porcine polymorphonuclear leukocytes (pmnls). one mechanism of this resistance seemed to be inhibition of the oxidative response as observed following induction of pmnls by phorbol myristate acetate (pma) and treatment with bacteria possessing the f165(1) fimbriae. in order to confirm whether or not the f165(1) fimbriae are involved in this inhibition, we evaluated the ef ... | 1999 | 10459581 |
| micellar electrokinetic chromatography as a complementary method to sodium dodecyl sulfate-polyacrylamide gel electrophoresis for studying limited proteolysis of proteins. | micellar electrokinetic chromatography (mekc) has been utilized as an analytical method to perform investigations on limited proteolysis of proteins. to this purpose partial proteolysis experiments with a series of proteinases were performed, utilizing as model protein pyruvate kinase (pk) from escherichia coli, an enzyme that is regulated allosterically by fructose 1,6-bisphosphate (fbp). data obtained with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and mekc were compa ... | 1999 | 10499331 |
| prevalence of virulence factors of escherichia coli strains isolated from diarrheic and healthy piglets after weaning. | this study determined the prevalence of f4, f5, f6, f17 and f41 fimbriae and the genes for feda (f18 fimbriae), lt and st enterotoxins, and shiga toxins stx1, stx2 and stx2e among e. coli isolated from 372 weaned pigs with diarrhea and 46 healthy pigs of the same age. agglutination tests showed that most isolates were negative for all five fimbrial antigens. the f4 antigen was found in 71 (19.1%) and the f5, f6, or f41 antigen was detected in 6.4% of isolates from diseased pigs. genes for the f1 ... | 1999 | 10510040 |
| seroprevalence of f4+ enterotoxigenic escherichia coli in regions with different pig farm densities. | sera of young sows from 135 closed belgian pig breeding farms were examined for the presence of serum antibodies specific for the f4 fimbrial antigen of enterotoxigenic escherichia coli (etec). since 80% of all pig farms in belgium are located in the provinces west-vlaanderen (44%, approximately 18 farms/10 km2), oost-vlaanderen (20%, approximately 9 farms/10 km2), antwerpen (10%, approximately 5 farms/10 km2) and vlaams-brabant (6%, approximately 3 farms/10 km2), the farms examined were randoml ... | 1999 | 10512045 |
| determination of the binding site on the extracellular domain of guanylyl cyclase c to heat-stable enterotoxin. | guanylyl cyclase c, one of the family of membrane-bound guanylyl cyclases, consists of an extracellular domain and an intracellular domain, which are connected by a single transmembrane polypeptide. the extracellular domain binds unique small polypeptides with high specificity, which include the endogenous peptide hormones, guanylin and uroguanylin, as well as an exogenous enterotoxigenic peptide, heat-stable enterotoxin, secreted by pathogenic escherichia coli. information on this specific bind ... | 1999 | 10531382 |
| porcine pulmonary surfactant preparations contain the antibacterial peptide prophenin and a c-terminal 18-residue fragment thereof. | surfactant preparations obtained from porcine lungs by extraction with chloroform/methanol followed by chromatography over lipidex-5000 are used for treatment of respiratory distress syndrome in preterm infants. these preparations contain about 98% phospholipids and 1-2% of the hydrophobic pulmonary surfactant-associated proteins b and c (sp-b and sp-c). separation of the proteins in the surfactant preparation by reversed-phase high performance liquid chromatography revealed, in addition to sp-b ... | 1999 | 10544246 |
| identification of rna-binding surfaces in iron regulatory protein-1. | post-transcriptional regulation of mrna translation and stability in iron metabolism involves the interaction between the trans-acting cytoplasmic iron regulatory proteins (irp-1 and irp-2) and cis-acting iron-responsive elements (ires) in mrna 5'- or 3'-untranslated regions. irp-1 can adopt two conformations: one with a [4fe-4s]-cluster, unable to bind ires, which functions as a cytoplasmic aconitase; one lacking this cluster, which accumulates in iron-deprived cells and binds mrna firmly. we i ... | 1999 | 10545118 |
| novel streptomycin and spectinomycin resistance gene as a gene cassette within a class 1 integron isolated from escherichia coli. | the aada genes, encoding resistance to streptomycin and spectinomycin, have been found as gene cassettes in different gram-negative and gram-positive bacterial species. the present study has revealed the sequence of a new gene, aada5, integrated as a gene cassette together with the trimethoprim resistance gene dfr7 in a class 1 integron. the integron was located on a plasmid and was identified in a pathogenic porcine escherichia coli isolate. | 1999 | 10582907 |
| ribosomal protein l7 included in tuberculin purified protein derivative (ppd) is a major heat-resistant protein inducing strong delayed-type hypersensitivity. | the tuberculin purified protein derivative (ppd) is a widely used diagnostic antigen for tuberculosis. it consists of more than 100 denatured proteins in a culture filtrate of a heated culture of mycobacterium tuberculosis. in two-dimensional electrophoretic analysis of ppds from m. tuberculosis and m. bovis bcg, most proteins were diffusely separated and could not be seen as spots because of denaturation, whereas a few proteins showed relatively clear spots, indicating heat resistance. two such ... | 1999 | 10607306 |
| the effect of probiotic lsp 122 on the control of post-weaning diarrhoea syndrome of piglets. | post-weaning diarrhoea syndrome (pwds) of piglets is caused mainly by enterotoxigenic escherichia coli (etec) strains. a new in-feed probiotic, lsp 122 (alpharma), containing viable spores of bacillus licheniformis was tested for its efficacy to control pwds in piglets in a low health-status farm, using four groups with a total of 256 weaned piglets for a 28-day period. one group (negative control) was offered antimicrobial-free and probiotics-free fed, one group was offered feed supplemented wi ... | 1999 | 10607501 |
| adverse effects of soluble non-starch polysaccharide (guar gum) on piglet growth and experimental colibacillosis immediately after weaning. | this study evaluated the effects of adding soluble fibre to the diet of healthy weaner pigs and weaner pigs experimentally infected with enterotoxigenic escherichia coli (etec) in a model of post-weaning colibacillosis. bodyweight gain, intestinal changes and proliferation of etec were measured 7 days following weaning. the basal diet consisted of pregelatinised rice fortified with animal protein. addition of guar gum to this diet elevated the soluble fibre content from 1 to 6 per cent, and was ... | 1999 | 10607504 |
| prevalence of shiga toxin genes among escherichia coli strains isolated from pigs. | 1999 | 10609576 | |
| localization of heat shock proteins in clinical actinobacillus actinomycetemcomitans strains and their effects on epithelial cell proliferation. | actinobacillus actinomycetemcomitans is an important pathogen in periodontitis. in the present study we localized the groel- and dnak-like heat shock proteins (hsp) in subcellular fractions of 12 a. actinomycetemcomitans strains of various clinical origin and compared their effects on periodontal epithelial cell proliferation and viability. in all strains, groel-like protein was found in the membrane, cytoplasm, and periplasm, whereas dnak-like protein was present in the cytoplasm and periplasm. ... | 2000 | 10620671 |
| microtubule formation and kinesin-driven microtubule gliding in vitro in the presence of lipopolysaccharide. | lipopolysaccharide (lps) is a main trigger substance for the development of septic shock and multiple organ failure. we showed by turbidity measurements that lps inhibits microtubule formation in a ph-dependent manner. inhibition was found to be not only due to sequestration of map2 by lps, but also of map1 and tau maps, indicating that lps is able to react with a broad variety of maps. lps-induced inhibition of microtubule formation could be compensated by additional tau or by addition of taxol ... | 1999 | 10623422 |
| challenge differentially affects cytokine production and metabolic status of growing and finishing swine. | growing (35 kg body weight) and finishing (85 kg body weight) swine challenged with endotoxin (escherichia coli o55:b5) at a dose of either 2 or 20 microg/kg produced tumor necrosis factor (tnf)alpha in a dose-response relationship as measured by bioassay. peak tnfalpha plasma levels were observed 1-2 hr post-challenge, returning to basal values 4 hr post-challenge. however, both an enzyme-linked immunosorbent assay specific for swine tnfalpha and total human tnfalpha demonstrated no dose-respon ... | 1999 | 10628426 |
| characterization of the african swine fever virus protein p49: a new late structural polypeptide. | the open reading frame b438l, located within the ecori b fragment of the african swine fever virus genome, is predicted to encode a protein of 438 amino acids with a molecular mass of 49.3 kda. it presents a cell attachment rgd (arg-gly-asp) motif but no other significant similarity to protein sequences in databases. northern blot and primer extension analysis showed that b438l is transcribed only at late times during virus infection. the b438l gene product has been expressed in escherichia coli ... | 2000 | 10640542 |
| comparison of membrane filtration rates and hydrophobic grid membrane filter coliform and escherichia coli counts in food suspensions using paddle-type and pulsifier sample preparation procedures. | food suspensions prepared by pulsifier contained less debris and filtered 1.3x to 12x faster through hydrophobic grid membrane filters (hgmfs) than those prepared by stomacher 400. coliform and escherichia coli counts made by an hgmf method yielded 84 and 36 paired samples, respectively, positive by both suspending methods. overall counts of pulsificates and stomachates did not differ significantly for either analysis, though coliform counts by pulsifier were significantly higher in mushrooms an ... | 2000 | 10643783 |
| characterization of integrons in escherichia coli of the normal intestinal flora of swine. | multiresistant escherichia coli isolates of the normal intestinal flora of healthy fattening pigs were examined for the presence of integron class 1 by xl (extra long) pcr. the class 1 integron was detected in 17 isolates originating from 14 healthy animals on seven different farms. one isolate contained two class 1 integrons. the inserted gene cassettes were characterized by dna sequencing and pcr. the ant(3")-ia gene responsible for resistance to streptomycin/spectinomycin was inserted in all ... | 1999 | 10647086 |
| house dust induces il-6 and il-8 response in a549 epithelial cells. | the in vitro potency of house dust to induce cytokine response in a549 lung epithelial cells was studied. dusts collected from carpet, bed, shelf and floor of a villa and an apartment by vacuuming were found to trigger the production of interleukin-8 (il-8) and interleukin-6 (il-6) in a dose-dependent manner, and the interleukin production was several-fold higher than of swine dust (used as a positive control). the il-8 and il-6 production of pure escherichia coli lipopolysaccharide was signific ... | 1999 | 10649856 |
| biological activities of lipopolysaccharides extracted from porcine vaccine strains. | lipopolysaccharides (lpss) were purified from actinobacillus pleuropneumoniae serotype 2, bordetella bronchiseptica and haemophilus parasuis serotype 5, which were used for vaccine production in japan, by the phenol-water procedure. in sds-page analysis, a. pleuropneumoniae lps, as well as escherichia coli lps, demonstrated a typical ladder profile of a smooth-type lps. on the other hand, b. bronchiseptica and h. parasuis lpss lacked the ladder profiles. it was found that the biological activity ... | 1999 | 10651044 |
| comparative pathology of bacterial enteric diseases of swine. | enteric bacterial infections are among the most common and economically significant diseases affecting swine production worldwide. clinical signs of these infections include diarrhea, reduced growth rate, weight loss, and death of preweaned, weanling, grower-finisher, young and adult age breeding animals. the most common etiological agents include escherichia coli, clostridium perfringens, lawsonia intracellularis, salmonella enterica, and brachyspira (serpulina) spp. with the exception of brach ... | 1999 | 10659346 |
| ultrastructure and dna fragmentation analysis of arterioles in swine infected with shiga toxin-producing escherichia coli. | shiga toxins (stx) produced by e. coli are potent cytotoxins that affect the vascular system. in humans, systemic toxemia causes renal glomerular damage manifested as hemolytic uremic syndrome. in swine, stx-producing e. coli (stec) cause edema disease that is characterized microscopically by segmental arteriolar smooth muscle cell (smc) lesions. our objectives were to characterize ultrastructurally and by tunel the type of death (apoptosis or necrosis) that occurs in smcs during edema disease. ... | 1999 | 10659354 |
| potentiation of the effectiveness of lactobacillus casei in the prevention of e. coli induced diarrhea in conventional and gnotobiotic pigs. | the influence of preventive administration of lactobacillus casei subsp. casei and maltodextrin kms x-70 on escherichia coli 08: k88 adhesion in the gastrointestinal tract of 11 conventional and 6 gnotobiotic piglets was investigated. the preventive administration of l. casei alone had almost no inhibitory effect on the adherence of e. coli to the jejunal mucosa of gnotobiotic and conventional piglets while the lactobacilli administered together with maltodextrin decreased the number of e. coli ... | 1999 | 10659357 |
| cloning and expression of glycolipid transfer protein from bovine and porcine brain. | glycolipid transfer protein (gltp) is a small (23-24 kda), basic protein (pi congruent with 9.0) that accelerates the intermembrane transfer of various glycolipids. here, we report the first cloning of cdnas that encode the bovine and porcine gltps. the cdna open reading frame for bovine gltp was constructed by bridge-overlapping extension polymerase chain reaction (pcr) after obtaining partial coding cdna clones by hot start, seminested, and rapid amplification of cdna ends-pcr. the cdna open r ... | 2000 | 10671554 |
| specificity of the wound-induced leucine aminopeptidase (lap-a) of tomato activity on dipeptide and tripeptide substrates. | wounding of tomato leaves results in the accumulation of an exoprotease called leucine aminopeptidase (lap-a) that preferentially hydrolyzes amino acid-p-nitroanilide and -beta-naphthylamide substrates with n-terminal leu, met and arg residues. to determine the substrate specificity of lap-a on more natural substrates, the rates of hydrolysis of 60 dipeptide and seven tripeptide substrates were determined. for comparison, the specificities of the porcine and escherichia coli laps were evaluated ... | 2000 | 10672029 |
| identification by mutagenesis of arginines in the substrate binding site of the porcine nadp-dependent isocitrate dehydrogenase. | pig heart mitochondrial nadp-dependent isocitrate dehydrogenase is the most extensively studied among the mammalian isocitrate dehydrogenases. the crystal structure of escherichia coli isocitrate dehydrogenase and sequence alignment of porcine with e. coli isocitrate dehydrogenase suggests that the porcine arg(101), arg(110), arg(120), and arg(133) are candidates for roles in substrate binding. the four arginines were separately mutated to glutamine using a polymerase chain reaction method. wild ... | 2000 | 10681542 |
| isolation and characterization of mini-tn10 lipopolysaccharide mutants of actinobacillus pleuropneumoniae serotype 1. | lipopolysaccharide (lps) has previously been identified as the major adhesin of actinobacillus pleuropneumoniae involved in adherence to porcine respiratory tract cells. the purpose of the present study was to isolate and characterize mutants in lps biosynthesis by using a mini-tn10 transposon mutagenesis system. seven mutants appeared to possess a rough lps (among which two had similar southern blot profiles) while one mutant (#5.1) expressed the high-molecular-mass lps, but as visualized by tr ... | 1999 | 10696481 |
| diarrhea in growing-finishing swine. | regardless of the etiology of an enteric disease in nursery age to finisher swine, making a prompt and accurate diagnosis is crucial. eliciting a complete history, assessing clinical signs and pathology, and selecting and interpreting laboratory tests are essential components in achieving this. early detection and diagnosis of enteric disease is particularly critical in the nursery through finisher phase because of economic impacts. recurrent topics when discussing control and prevention of ente ... | 2000 | 10707417 |
| helicobacter pylori possesses two chey response regulators and a histidine kinase sensor, chea, which are essential for chemotaxis and colonization of the gastric mucosa. | infection of the mucous layer of the human stomach by helicobacter pylori requires the bacterium to be motile and presumably chemotactic. previous studies have shown that fully functional flagella are essential for motility and colonization, but the role of chemotaxis remains unclear. the two-component regulatory system chea/chey has been shown to play a major role in chemotaxis in other enteric bacteria. scrutiny of the 26695 genome sequence suggests that h. pylori has two chey response regulat ... | 2000 | 10722597 |
| [action of the vaccination of sows against e. coli infections with a subsequent postpartum booster]. | in an eastern european pig productions unit with high prevalence of suckling piglets diarrhoea during late lactation the following trial was conducted: the sows were assigned to an experimental and to a control group and were treated as follows: group one (15 sows) were vaccinated with a single 2 ml dose of porcovac plus (höchst roussel vet.) during their late pregnancy (gilts were vaccinated twice). booster vaccination was performed between day 2-7 p.p. group two (15 sows) were vaccinated durin ... | 2000 | 10726365 |
| functional consequences of the deletion mutation deltaglu160 in human cardiac troponin t. | to explore the functional consequences of a deletion mutation of troponin t (deltaglu160) found in familial hypertrophic cardiomyopathy, the mutant human cardiac troponin t, and wild-type troponins t, i, and c were expressed in escherichia coli and directly incorporated into isolated porcine cardiac myofibrils using our previously reported troponin exchange technique. the mutant troponin t showed a slightly reduced potency in replacing the endogenous troponin complex in myofibrils and did not af ... | 2000 | 10731693 |
| epidemiological study of pap genes among diarrheagenic or septicemic escherichia coli strains producing cs31a and f17 adhesins and characterization of pap(31a) fimbriae. | the association of the pap operon with the cs31a and f17 adhesins was studied with 255 escherichia coli strains isolated from calves, lambs, or humans with diarrhea. the three classes of papg adhesin with different receptor binding preferences were also screened. the pap operon was associated with 50 and 36% of human strains that produced cs31a and ovine strains that produced f17, respectively. among the bovine isolates, the pap operon was detected in 61% of the cs31a-positive isolates and 72% o ... | 2000 | 10747134 |
| a porcine model of sepsis resulting from the combined insults of hemorrhage and peritonitis. | the physiological responses to either hemorrhage or sepsis have been well documented, however, their simultaneous delivery, as often seen in penetrating trauma, has not been extensively studied. a terminally-anesthetized porcine model of fixed-volume hemorrhage combined with intraperitoneal sepsis was developed. large white pigs (45-60 kg) were bled 40% of blood volume and peritonitis was induced using an e. coil (o18:k1:h7) culture. three groups of animals were sequentially studied. group a (n ... | 2000 | 10774618 |
| identification of the f17 fimbrial subunit- and adhesin-encoding (f17a and f17g) gene variants in necrotoxigenic escherichia coli from cattle, pigs and humans. | putative colonization factors of the f17 family of fimbrial adhesins have been identified in necrotoxigenic escherichia coli type 1 and type 2 (ntec1 and ntec2) from calves, pigs, and humans. the f17a and f17g gene variants, coding respectively for the major subunit and for the adhesin of the f17 fimbriae, were typed in 70 e. coli carrying f17-related sequences (15 ntec1, 51 ntec2, and four non-ntec) by colony hybridisation with gene probes derived from the different f17a gene variants (a, b, c, ... | 2000 | 10781731 |
| effects of heat stress on the antimicrobial drug resistance of escherichia coli of the intestinal flora of swine. | the effects of heat stress on the antimicrobial drug resistance of escherichia coli of the intestinal tract of swine were studied in animals from a farm that had not been supplementing antimicrobials in feed for the past 10 years. in one study, 10 finisher hogs were heat stressed (34 degrees c) for 24 h. antimicrobial resistance levels after stress were significantly higher (p < 0.05) when compared with pre-stress levels for amikacin, ampicillin, cephalothin, neomycin and tetracycline from faeca ... | 2000 | 10792544 |
| antibiotic resistance monitoring: the spanish programme. the vav network. red de vigilancia de resistencias antibióticas en bacterias de origen veterinario. | antimicrobial resistance is a problem in modern public health and antimicrobial use and especially misuse, the most important selecting force for bacterial antibiotic resistance. as this resistance must be monitored we have designed the spanish network 'red de vigilancia de resistencias antibióticas en bacterias de origen veterinario'. this network covers the three critical points of veterinary responsibility, bacteria from sick animals, bacteria from healthy animals and bacteria from food anima ... | 2000 | 10794948 |
| ecological impact of antibiotic use in animals on different complex microflora: environment. | different means of interaction between microecological systems in different animal hosts (including humans) and the environment may occur during the transfer of resistant bacteria and their resistance genes. spread of resistance takes place in different ways with respect to clonal spread of resistance strains by the spread of wide host range plasmids and translocatable elements. commensals in ecosystems have a special significance and a pronounced capacity for acquisition and transfer of resista ... | 2000 | 10794954 |
| biosynthesis of isoprenoids in escherichia coli: stereochemistry of the reaction catalyzed by farnesyl diphosphate synthase. | [formula: see text] farnesyl diphosphate (fpp) synthase from escherichia coli catalyzes the condensation of isopentenyl diphosphate (ipp) and geranyl diphosphate (gpp) with selective removal of the pro-r hydrogen at c2 of ipp, the same stereochemistry observed for the pig liver, yeast, and avian enzymes. | 1999 | 10825960 |
| use of chlorhexidine-releasing nylon fibres to reduce device-related uterine infections. | implantation of intrauterine devices (iuds) is associated with an increased incidence of uterine infection, probably as a result of vaginal bacteria entering the uterus at the time of insertion. to reduce the incidence of iud-related infections, the incorporation of antimicrobial agents into the tail of the device was studied. chlorhexidine was shown to be released from within nylon hollow fibres at a rate of approximately 114 microg x day(-1) for 10 days. this rate of release was sufficient to ... | 2000 | 10828710 |
| an unusual presentation of suspected oedema disease of swine in kenya. | from a group of 11 recently weaned pigs, 4 were reported to be sick. clinical examination of the sick pigs revealed marked dyspnoea, bluish-red discolouration of the skin, incoordination and difficulty in walking. bacteriological examination of the gut contents of 2 pigs that had died earlier yielded pure cultures of haemolytic escherichia coli. post mortem examination of the remaining 2 pigs that died subsequently revealed progressive pulmonary collapse. one of these also showed subcutaneous oe ... | 1999 | 10855832 |
| competitive elisa for detection of antibodies to porcine reproductive and respiratory syndrome virus using recombinant e. coli-expressed nucleocapsid protein as antigen. | the 15 kda nucleocapsid (n) protein is the most abundant protein of the porcine reproductive and respiratory syndrome virus (prrsv), and is highly antigenic, which therefore makes it a suitable candidate for the detection of virus-specific antibodies and diagnosis of the disease. in this study, complementary dna corresponding to the entire n gene of the iaf-klop strain of prrsv was cloned into the pgex-4t-1 vector, and the n protein was expressed in escherichia coli fused to the glutathione s-tr ... | 2000 | 10856758 |
| molecular cloning of endo-beta -galactosidase c and its application in removing alpha -galactosyl xenoantigen from blood vessels in the pig kidney. | galalpha1-3gal is the major xenoantigenic epitope responsible for hyperacute rejection upon pig to human xenotransplantation. endo-beta-galactosidase c from clostridium perfringens destroys the antigenic epitope by cleaving the beta-galactosidic linkage in the galalpha1-3galbeta1-4glcnac structure. based on partial peptide sequences of the enzyme, we molecularly cloned the enzyme gene, which encodes a protein with a predicted molecular mass of about 93 kda. the deduced protein sequence of the en ... | 2000 | 10858461 |
| immunoblot assays using recombinant antigens for the detection of mycoplasma hyopneumoniae antibodies. | the 36kda l-lactate dehydrogenase (ldh) and a 29kda partial fragment of an abc transporter atp-binding protein analogue/multidrug resistance protein homologue (pr2) of mycoplasma hyopneumoniae were tested for their potential as diagnostic antigens. recombinant ldh was genetically engineered to contain six histidine residues at its c-terminal end, expressed in escherichia coli and purified to a high degree using ni(2+)-chelate affinity chromatography. a partial 262 amino acid segment representing ... | 2000 | 10865156 |
| phosphorylated and dephosphorylated structures of pig heart, gtp-specific succinyl-coa synthetase. | succinyl-coa synthetase (scs) catalyzes the reversible phosphorylation/dephosphorylation reaction:¿¿¿rm succinyl ¿hbox ¿-¿coa+ndp+p_i¿leftrightarrow succinate+coa+ntp¿¿where n denotes adenosine or guanosine. in the course of the reaction, an essential histidine residue is transiently phosphorylated. we have crystallized and solved the structure of the gtp-specific isoform of scs from pig heart (ec 6.2.1.4) in both the dephosphorylated and phosphorylated forms. the structures were refined to 2.1 ... | 2000 | 10873456 |
| impaired sarcoplasmic calcium release inhibits myocardial contraction in experimental sepsis. | in septic shock, myocardial dysfunction develops over the course of illness, but the mechanism of this depression is not clear. in this study, mechanisms of myocardial dysfunction were examined in a porcine model of escherichia coli sepsis. | 2000 | 10877367 |
| species-specific monoclonal antibodies to escherichia coli-expressed p36 cytosolic protein of mycoplasma hyopneumoniae. | the p36 protein of mycoplasma hyopneumoniae is a cytosolic protein carrying species-specific antigenic determinants. based on the genomic sequence of the reference strain atcc 25934, primers were designed for pcr amplification of the p36-encoding gene (948 bp). these primers were shown to be specific to m. hyopneumoniae since no dna amplicons could be obtained with other mycoplasma species and pathogenic bacteria that commonly colonize the porcine respiratory tract. the amplified p36 gene was su ... | 2000 | 10882646 |
| antimicrobial resistance in enteric porcine escherichia coli strains in spain. | 2000 | 10887984 | |
| cloning and expression of a cdna coding for catalase from zebrafish (danio rerio). | a full-length complementary dna (cdna) clone encoding a catalase was amplified by the rapid amplication of cdna ends-polymerase chain reaction (race-pcr) technique from zebrafish (danio rerio) mrna. nucleotide sequence analysis of this cdna clone revealed that it comprised a complete open reading frame coding for 526 amino acid residues and that it had a molecular mass of 59 654 da. the deduced amino acid sequence showed high similarity with the sequences of catalase from swine (86.9%), mouse (8 ... | 2000 | 10888504 |
| norepinephrine and n(g)-monomethyl-l-arginine in hyperdynamic septic shock in pigs: effects on intestinal oxygen exchange and energy balance. | to compare the effects of norepinephrine (nor) and the nonselective nitric oxide synthase inhibitor, n(g)-monomethyl-l-arginine (l-nmma), on intestinal blood flow, oxygen exchange, and energy metabolism over 24 hrs of hyperdynamic, normotensive porcine endotoxic shock. | 2000 | 10890656 |
| analysis of promoter sequences from lactobacillus and lactococcus and their activity in several lactobacillus species. | promoter-active fragments were isolated from the genome of the probiotic organism lactobacillus rhamnosus strain gg using the promoter-probe vector pnz272. these promoter elements, together with a promoter fragment isolated from the vaginal strain lactobacillus fermentum br11 and two previously defined promoters (lactococcus lactis and lactobacillus acidophilus atcc 4356 slpa), were introduced into three strains of lactobacillus. primer-extension analysis was used to map the transcriptional star ... | 2000 | 10896218 |
| trauma- and sepsis-induced hepatic ischemia and reperfusion injury: role of angiotensin ii. | we hypothesized that angiotensin ii, a potent vasoconstrictor, is involved in the occurrence of hepatic ischemia after burn and sepsis, and that administration of angiotensin ii antagonist dup753 would ameliorate this process. | 2000 | 10896368 |
| cloning, sequencing, and expression of porcine interleukin-18 in escherichia coli. | il-18 is the new name of a novel cytokine that plays an important role in t(h1) response, primarily by its ability to induce ifn-gamma production in t cells and natural killer cells. the porcine il-18 gene was isolated using rt-pcr from porcine alveolar macrophages. sequence analysis of the porcine il-18 gene has demonstrated an open reading frame of 579 base pairs encoding 192 amino acids precursor protein with a predicted molecular mass of 22 kda. the porcine il-18 gene shares 84% and 89% simi ... | 2000 | 10901174 |
| involvement of apoptosis in the endotoxemic lesions of the liver and kidneys of piglets. | the involvement of apoptosis was evaluated in lesions of endotoxemic piglets. a single injection with e. coli o111:b4 lipopolysaccharide (lps) induced foci of coagulative necrosis in the liver and kidneys. no significant change was observed in these organs at 1.5 hr after lps injection, but at 6 hr, epithelial cells with chromatin condensation or fragmentation and apoptotic bodies were visible. foci of coagulative necrosis were formed within 24 hr after lps inoculation. in and adjacent to the ne ... | 2000 | 10907689 |
| molecular cloning and characterization of a unique 60 kda/72 kda antigen gene encoding enzyme i of the phosphoenolpyruvate: sugar phosphotransferase system (pts) of mycoplasma hyopneumoniae. | the recombinant clone expressing a 60 kda (p60) antigen was isolated from escherichia coli by screening a lambda embl3 genomic library using rabbit produced antiserum against mycoplasma hyopneumoniae. sequence analysis revealed that an interrupted (by a uga codon) open reading frame coding for a 72 kda protein (p72) may contain the p60 antigen gene. western blot analysis with an anti-p60 monospecific antibody confirmed the presence of a p72 antigen from the total protein of m. hyopneumoniae, and ... | 2000 | 10920262 |
| pathogenic characteristics of escherichia coli strains isolated from newborn piglets with diarrhea in brazil. | ninety-one escherichia coli isolates obtained from diarrheic and normal feces of newborn piglets (0-11 days of age) from three states of brazil were assessed for phenotypic and genotypic characteristics associated with pathogenic processes. these isolates expressed fimbriae f18ac and type 1, but not fimbriae k88, k99, 987p or f41. genes for toxins (lt-i, sta, slt-i, slt-ii, slt-iiv) either individually or combined were found to be present in most of the diarrheic strains (65.7%) and in 42.8% of ... | 2000 | 10925041 |
| frequency of adhesive factors and enterotoxins in strains of escherichia coli isolated from piglets with diarrhea. | neonatal colibacillosis is one of the most prevalent illnesses in pig farms. in order to examine the frequency of adherence factors and the production of enterotoxins in strains of e. coli, we collected stool specimens from 500 piglets between 1 and 10 days of age with diarrhea, including piglets from several different farms on the periphery of mérida, yucatán. one thousand and eighty (1080) strains of e. coli were isolated, of which 127 (11.76%) produced sta, and 62 (5.74%) produced adherence f ... | 1997 | 10932724 |
| hemolysins and verotoxin (vt) in enteric escherichia coli isolated from pigs. | one hundred and three e. coli strains isolated from the intestinal contents of pigs were examined for hemolysis and verotoxin production on vero monolayer cells. hemolysins were produced by 18 (17.5%) strains;--hemolysin was produced by 6 strains of which 4 belonged to serotype o149:k91, k88ac;--hemolysin was produced by 8 strains which could no be serotyped; 1 strain produced-hemolysin and possessed fimbrial antigens k88; the remaining 3 strains were enterohemolytic and vt producers. among the ... | 1997 | 10932726 |
| the effect of cvvhd and endotoxin on the oxidative burst, adhesion molecules and distribution in tissues of granulocytes. | extracorporeal circulation, such as cardiopulmonary bypass and haemodialysis, has been associated with an activation of the immune system, especially the granulocytes. continuous veno-venous haemodiafiltration (cvvhd) is used in critically ill septic patients. during cvvhd cytokines are excreted in the ultrafiltrate. but when the membranes used in cvvhd are cultured with granulocytes, the granulocytes are slightly activated. this effect is potentiated by endotoxin. we therefore, in vivo, compare ... | 2000 | 10945396 |
| cloning, post-translational modifications, heterologous expression and ligand-binding of boar salivary lipocalin. | boar submaxillary glands produce the sex-specific salivary lipocalin (sal), which binds steroidal sex pheromones as endogenous ligands. the cdna encoding sal was cloned and sequenced. from a single individual, two protein isoforms, differing in three amino acid residues, were purified and structurally characterized by a combined edman degradation/ms approach. these experiments ascertained that the mature polypeptide is composed of 168 amino acid residues, that one of the three putative glycosyla ... | 2000 | 10947950 |
| identification of the gene encoding bmpb, a 30 kda outer envelope lipoprotein of brachyspira (serpulina) hyodysenteriae, and immunogenicity of recombinant bmpb in mice and pigs. | a gene encoding a 30kda outer envelope protein of the intestinal spirochaete brachyspira (serpulina) hyodysenteriae, was cloned and expressed in escherichia coli strain xlolr. five phagemids containing dna inserts encoding the protein were established and one clone (psha) was sequenced. an 816bp hypothetical open reading frame (orf) was identified, with a potential ribosome binding site (aggag), and putative -10 (tataat) and -35 (ttgaaa) promoter regions upstream from the atg start of the orf. a ... | 2000 | 10973699 |
| expression of trigonopsis variabilis d-amino acid oxidase gene in escherichia coli and characterization of its inactive mutants. | the d-amino acid oxidase cdna gene (daao) of trigonopsis variabilis was prepared by reverse transcriptase-polymerase chain reaction (pcr) and cloned into escherichia coli expression vector, ptrc99a, under the control of tac promoter. expression of daao gene significantly affected the growth and morphology of e. coli. the highest d-amino acid oxidase (daao) activity was 705 u (mg of protein)(-)(1), which was about 12-fold higher than that of d-alanine-induced t. variabilis. the daao protein exhib ... | 2000 | 10978770 |
| cloning, expression and characterisation of a recombinant triosephosphate isomerase from taenia solium. | we isolated and characterised the cdna that encodes the glycolytic enzyme, triosephosphate isomerase from taenia solium. a 450 bp dna fragment was obtained by the polymerase chain reaction using a cdna from larval stage as template and degenerate oligonucleotides designed from conserved polypeptide sequences from tpis of several organisms. the fragment was used to screen a t. solium larval stage cdna library. the isolated cdna, encoding a protein of 250 amino acids shares 44.8-59.6% positional i ... | 2000 | 10980291 |
| [the immunomodulating properties of translam]. | effect of translam--beta-1,3;1,6-d-glucan on some of naturally occurring host protective mechanisms was investigated. it was shown that translam has potent biological activity: it has preventive effect on experimental bacterial infections, it stimulates absorbtion and digestion activity of mononuclear phagocytes, it effects on humoral and cell immunity. translam had mitogenic activity. | 2000 | 10987100 |
| incorporation of nitrotyrosine into alpha-tubulin by recombinant mammalian tubulin-tyrosine ligase. | tubulin-tyrosine ligase (ttl, ec 6.3.2.25) from porcine brain, which catalyses the readdition of tyrosine to the c-terminus of detyrosinated alpha-tubulin, was cloned and expressed in escherichia coli as a glutathione s-transferase-fusion protein. upon cleavage of the immobilised fusion protein, an electrophoretically homogeneous enzyme was obtained. recombinant ttl, which exhibited similar catalytic properties as the mammalian enzyme purified from brain tissue, was capable of using nitrotyrosin ... | 2000 | 11004583 |
| 24-hydroxycholesterol is a substrate for hepatic cholesterol 7alpha-hydroxylase (cyp7a). | (24s)-hydroxycholesterol is formed from cholesterol in the brain and is important for cholesterol homeostasis in this organ. elimination of (24s)-hydroxycholesterol has been suggested to occur in the liver but little is known about the metabolism of this oxysterol. in the present investigation, we report formation of 7alpha, 24-dihydroxycholesterol in pig and human liver. 7alpha-hydroxylase activity toward both isomers of 24-hydroxycholesterol [(24s) and (24r)] was found in a partially purified ... | 2000 | 11013305 |
| inhibition of enterotoxin-induced porcine colonic secretion by diarylsulfonylureas in vitro. | muscle-stripped piglet colon was used to evaluate changes in short-circuit current (i(sc)) as an indicator of anion secretion. mucosal exposure to escherichia coli heat-stable (sta) or heat-labile enterotoxins (lt) stimulated i(sc) by 32 +/- 5 and 42 +/- 7 microa/cm(2), respectively. enterotoxin-stimulated i(sc) was not significantly affected by either 4,4'-diaminostilbene-2, 2'-disulfonic acid or cdcl(2), inhibitors of ca(2+)-activated cl(-) channels and clc-2 channels, respectively. alternativ ... | 2000 | 11053008 |
| cloning, expression and characterization of the pig liver gdp-mannose pyrophosphorylase. evidence that gdp-mannose and gdp-glc pyrophosphorylases are different proteins. | gdp-man, the mannosyl donor for most man-containing polymers is formed by the transfer of man-1-p to gtp to form gdp-man and ppi. this reaction is catalyzed by the widespread and essential enzyme, gdp-man pyrophosphorylase (gmpp). the pig liver gmpp consists of an alpha subunit (43 kda) and a beta subunit (37 kda). purified pig gmpp catalyzes the synthesis of gdp-glc (from glc-1-p and gtp) and gdp-man (from man-1-p and gtp), but has higher activity for the formation of gdp-glc than for synthesis ... | 2000 | 11082198 |
| chromium-induced glucose uptake, superoxide anion production, and phagocytosis in cultured pulmonary alveolar macrophages of weanling pigs. | the dose-dependent effects of chromium chloride (crcl3) and chromium picolinate (crpic) were evaluated for their glucose uptake, superoxide anion (o2-) production, activity of glucose-6-phosphate dehydrogenase, and phagocytosis of incubated pulmonary alveolar macrophages in medium containing no or 5 x 10(-8)m insulin. glucose uptake was found to increase in cells treated with 20 microg/l crcl3. incubation with 20 microg/l of crpic enhanced glucose uptake and o2- production in an insulin-dependen ... | 2000 | 11097471 |
| adenovirus-mediated triple gene transfer of human complement regulating proteins to the porcine endothelial cell. | 2000 | 11120272 | |
| hepatic o2 exchange and liver energy metabolism in hyperdynamic porcine endotoxemia: effects of iloprost. | to compare the effects of a 12 h continuous infusion of iloprost, a stable prostacyclin analogue, on hepatic blood flow (qliv), o2 exchange, and energy metabolism during a 24 h hyperdynamic, porcine endotoxemia with volume resuscitation alone. | 2000 | 11126268 |
| identification of escherichia coli recovered from milk of sows with coliform mastitis by random amplified polymorphic dna (rapd) using standardized reagents. | a standardized-reagents commercial kit for random amplified polymorphic dna (rapd) analysis was used for typing 58 escherichia coli strains that were recovered from the milk of sows, having coliform mastitis, within a single swineherd in sweden. previously, the 58 e. coli strains were characterized serologically and profiled biochemically. they were also evaluated for their serum resistance and their ability to adhere to fibronectin and bovine fetal fibroblasts. the rapd analysis was fast, easil ... | 2000 | 11126575 |
| inhibition of neutrophil elastase prevents cathelicidin activation and impairs clearance of bacteria from wounds. | the host defense roles of neutrophil elastase in a porcine skin wound chamber model were explored. analysis of wound fluid by acid-urea polyacrylamide gel electrophoresis, western blot, and bacterial overlay confirmed that the neutrophil-derived protegrins constituted the major stable antimicrobial polypeptide in the wound fluid. the application to the wound of 0.10 and 0.25 mm n-methoxysuccinyl-alanine-alanine-proline-valine (aapv) chloromethyl ketone, a specific neutrophil elastase inhibitor ( ... | 2001 | 11133774 |
| evidence for horizontal transfer of ssudat1i restriction-modification genes to the streptococcus suis genome. | different strains of streptococcus suis serotypes 1 and 2 isolated from pigs either contained a restriction-modification (r-m) system or lacked it. the r-m system was an isoschizomer of streptococcus pneumoniae dpnii, which recognizes nucleotide sequence 5'-gatc-3'. the nucleotide sequencing of the genes encoding the r-m system in s. suis dat1, designated ssudat1i, showed that the ssudat1i gene region contained two methyltransferase genes, designated ssuma and ssumb, as does the dpnii system. th ... | 2001 | 11133943 |
| phase variation of f165(1) (prs-like) fimbriae from escherichia coli causing septicaemia in animals. | escherichia coli o115:f165 strains are associated with septicaemia in young pigs and synthesize fimbriae involved in virulence, designated as f165(1). f165(1) fimbriae belong to the p fimbrial family and are encoded by the foo gene cluster. the foo regulatory region of strain 5131 possesses characteristics similar to that of members of the p regulatory family, including papi and papb homologues, and two gatc sites separated by 102 bp, targets of differential dam methylation. in wild-type strains ... | 2000 | 11142399 |
| novel genes affecting urease acivity in actinobacillus pleuropneumoniae. | characterization of a series of urease-negative transposon mutations of actinobacillus pleuropneumoniae revealed that many of the mutants had insertions 2 to 4 kbp upstream of the urease gene cluster. a 5-kbp upstream region of dna was sequenced and found to contain six open reading frames (orfs) transcribed in the same orientation as the urease genes. as well, a partial orf, apur, 202 bp upstream of these six orfs, is transcribed in the opposite orientation. the predicted product of this partia ... | 2001 | 11157936 |
| bacterial expression of an immunologically reactive pcv2 orf2 fusion protein. | the entire coding region of open reading frame 2 (orf2) of porcine circovirus 2 (pcv2) was linked to the 3'-end of the maltose-binding protein (mbp)-his(8)-tag gene. the fusion protein was expressed as soluble form after induction by isopropylthio-beta-d-galactoside. mbp-his(8)-orf2 was purified to homogeneity by immobilized metal affinity chromatography based on the interaction of the polyhistidine-tag with metal ions. expression could represent 1% of the total protein in escherichia coli, allo ... | 2001 | 11162395 |
| resistance patterns of non-o157 shiga toxin-producing escherichia coli (stec) strains isolated from animals, food and asymptomatic human carriers in switzerland. | the objective of the present study was to determine the resistance patterns of non-o157 shiga toxin-producing escherichia coli (stec) strains isolated from different sources in switzerland during the period 1997-99 as an epidemiological marker. | 2001 | 11169054 |
| occurrence and virulence factors of non-o157 shiga toxin-producing escherichia coli in retail meat in dunedin, new zealand. | retail raw meat was sampled for the presence of shiga toxin-producing escherichia coli (stec) using enrichment culture and vero cell assay. the stec obtained were serotyped and tested for enterohaemolysin (ehly) production and the eae gene. the presence of shiga toxin genes (stx) was confirmed by polymerase chain reaction. a total of 18 stec were isolated accounting for 12% of beef, 17% of lamb and 4% of pork samples. five isolates produced ehly but none possessed the eae gene. five isolates wer ... | 2001 | 11169055 |
| development of a competitive elisa using a truncated e2 recombinant protein as antigen for detection of antibodies to classical swine fever virus. | the sequence encoding a truncated e2 glycoprotein of the alfort/187 strain of classical swine fever virus (csfv) was expressed in escherichia coli using the pet expression system and the recombinant product purified by ni-nta agarose affinity chromatography. the antigenicity of this recombinant protein was demonstrated by immunoblot using anti- csfv-specific antibodies. a monoclonal antibody was produced against the truncated e2 protein and used as competitor in an elisa for the detection of ant ... | 2001 | 11170845 |
| bacteriocin production by lactobacillus salivarius of animal origin. | 2000 | 11184178 | |
| human lens thioltransferase: cloning, purification, and function. | to clone the human lens thioltransferase (ttase) gene and to purify, characterize and study the possible function of the recombinant human lens thioltransferase (rhlt). | 2001 | 11222536 |
| [action of natural gamma-interferons on functional activity of phagocytes and antibody synthesis after vaccination]. | natural swine and cattle gamma-ifns were prepared for trials. one dose of gamma-suiferon contained 1000 iu, that of gamma-boviferon--2000 iu. three series of researches were carried out to estimate the in vitro and in vivo absorbing activity of phagocytes (monocytes and neutrophiles), their bactericidal ability (on new born pigs and calves, 2 months old animals, sows and cows with calf) and antibodygenesis after immunization of animals by colibacteriosis vaccine. it has been shown in trials that ... | 2000 | 11247346 |
| sequence analysis of the aminoacylase-1 family. a new proposed signature for metalloexopeptidases. | the amino acid sequence analysis of the human and porcine aminoacylases-1, the carboxypeptidase s precursor from saccharomyces cerevisiae, the succinyl-diaminopimelate desuccinylase from escherichia coli, haemophilus influenzae and corynebacterium glutamicum, the acetylornithine deacetylase from escherichia coli and dictyostelium discoideum and the carboxypeptidase g(2) precursor from pseudomonas strain, using the basic local alignment search tool (blast) and the position-specific iterated blast ... | 2001 | 11250542 |
| phenylarsine oxide inhibits nitric oxide synthase in pulmonary artery endothelial cells. | the role of protein tyrosine phosphorylation during regulation of no synthase (enos) activity in endothelial cells is poorly understood. studies to define this role have used inhibitors of tyrosine kinase or tyrosine phosphatase (tp). phenylarsine oxide (pao), an inhibitor of tp, has been reported to bind thiol groups, and recent work from our laboratory demonstrates that enos activity depends on thiol groups at its catalytic site. therefore, we hypothesized that pao may have a direct effect on ... | 2000 | 11281283 |
| eae-negative attaching and effacing escherichia coli from piglets with diarrhea. | one hundred and ninety strains of escherichia coli that were isolated from pigs with diarrhea in the state of são paulo, brazil, and that were negative for enterotoxins and cytotoxins were investigated. strains which adhered to hela cells were examined for fluorescence actin staining (fas), the ability to induce attaching and effacing (a/e) lesions on hep-2 cells detectable by transmission electron microscopy and the presence of eae gene sequences detected by pcr. intimin production was detected ... | 2001 | 11281328 |
| mechanical ventilation-induced air-space enlargement during experimental pneumonia in piglets. | mechanical ventilation-induced air-space enlargement was investigated in a porcine model of multifocal pneumonia. following the intrabronchial inoculation of escherichia coli, 9 piglets (22 +/- 2 kg) were ventilated with a tidal volume (vt) of 15 ml/kg for 43 +/- 15 h. five noninoculated piglets ventilated for 60 h with the same vt served as control animals. following death, the lungs were fixed and lung morphometry was assessed. in inoculated animals, unventilated infected and normally ventilat ... | 2001 | 11282773 |
| vaccination with the glycoprotein d gene of pseudorabies virus delivered by nonpathogenic escherichia coli elicits protective immune responses. | attenuated intracellular bacteria, such as salmonella and shigella, have been exploited to act as gene delivery vectors. in this study, we report that nonpathogenic, live escherichia coli can be used for the delivery of dna vaccines in vivo, leading to generation of immune responses against plasmid-encoded foreign antigens. the pseudorabies virus (prv) dna vaccine carrying the glycoprotein d (gd) gene delivered by e. coli was able to induce protective immune responses in mice against a lethal pr ... | 2001 | 11312026 |