Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| a novel antimicrobial approach based on the inhibition of zinc uptake in salmonella enterica. | in this review we discuss evidences suggesting that bacterial zinc homeostasis represents a promising target for new antimicrobial strategies. the ability of the gut pathogen salmonella enterica sv typhimurium to withstand the host responses aimed at controlling growth of the pathogen critically depends on the zinc importer znuabc. strains lacking a functional znuabc or its soluble component znua display a dramatic loss of pathogenicity, due to a reduced ability to express virulence factors; wit ... | 2017 | 28636417 |
| gut microbiota, nitric oxide, and microglia as prerequisites for neurodegenerative disorders. | regulating fluctuating endogenous nitric oxide (no) levels is necessary for proper physiological functions. aberrant no pathways are implicated in a number of neurological disorders, including alzheimer's disease (ad) and parkinson's disease. the mechanism of no in oxidative and nitrosative stress with pathological consequences involves reactions with reactive oxygen species (e.g., superoxide) to form the highly reactive peroxynitrite, hydrogen peroxide, hypochloride ions and hydroxyl radical. n ... | 2017 | 28640632 |
| development of elisas for diagnosis of acute typhoid fever in nigerian children. | improved serodiagnostic tests for typhoid fever (tf) are needed for surveillance, to facilitate patient management, curb antibiotic resistance, and inform public health programs. to address this need, iga, igm and igg elisas using salmonella enterica serovar typhi (s. typhi) lipopolysaccharide (lps) and hemolysin e (t1477) protein were conducted on 86 nigerian pediatric tf and 29 non-typhoidal salmonella (nts) cases, 178 culture-negative febrile cases, 28 "other" (i.e., non-salmonella) pediatric ... | 2017 | 28640809 |
| the antimicrobial spectrum of xeroform(®). | xeroform(®) is a petrolatum-based fine mesh gauze containing 3% bismuth tribromophenate. bismuth, similar to other metals, has antimicrobial properties. xeroform(®) has been used for decades in burn and plastic surgery as a donor site dressing and as a covering for wounds or partial thickness burns. despite this, the antimicrobial spectrum of xeroform(®) remains largely unknown. we examined the in-vitro efficacy of xeroform(®) against common burn pathogens using zone-of-inhibition methodology in ... | 2017 | 28641915 |
| immunogenicity and protective efficacy of a salmonella enteritidis sptp mutant as a live attenuated vaccine candidate. | salmonella enterica serovar enteritidis (s. enteritidis) is a highly adaptive pathogen in both humans and animals. as a salmonella type iii secretion system (t3ss) effector, salmonella protein tyrosine phosphatase (sptp) is critical for virulence in this genus. to investigate the feasibility of using c50336δsptp as a live attenuated oral vaccine in mice, we generated the sptp gene deletion mutant c50336δsptp in s. enteritidis strain c50336 by λ-red mediated recombination and evaluated the protec ... | 2017 | 28646853 |
| the validation and implications of using whole genome sequencing as a replacement for traditional serotyping for a national salmonella reference laboratory. | salmonella serotyping remains the gold-standard tool for the classification of salmonella isolates and forms the basis of canada's national surveillance program for this priority foodborne pathogen. public health officials have been increasingly looking toward whole genome sequencing (wgs) to provide a large set of data from which all the relevant information about an isolate can be mined. however, rigorous validation and careful consideration of potential implications in the replacement of trad ... | 2017 | 28649236 |
| three-dimensional organotypic co-culture model of intestinal epithelial cells and macrophages to study salmonella enterica colonization patterns. | three-dimensional models of human intestinal epithelium mimic the differentiated form and function of parental tissues often not exhibited by two-dimensional monolayers and respond to salmonella in key ways that reflect in vivo infections. to further enhance the physiological relevance of three-dimensional models to more closely approximate in vivo intestinal microenvironments encountered by salmonella, we developed and validated a novel three-dimensional co-culture infection model of colonic ep ... | 2017 | 28649632 |
| coordinated expression of acrab-tolc and eight other functional efflux pumps through activating rama and mara in salmonella enterica serovar typhimurium. | the aim of this study was to determine the expression of eight other functional transporter genes upon acrab inactivation and also the expression of acrab when the function of eight other transporters are impaired in salmonella enterica. we used single- or multigene deletion mutants (i.e., δacra, δacrb, δtolc, δacrab, δacref, δacrd, δmdsabc, δmdtabc, δemrab, δmacab, δmdfa, δmdtk, δacrabrama, δacrabmara, and δacrabsoxs) and real time (rt)-pcr to quantify the expression of different pump and regul ... | 2017 | 28650690 |
| epigallocatechin gallate inhibits the type iii secretion system of gram-negative enteropathogenic bacteria under model conditions. | epigallocatechin gallate (egcg), a major polyphenol in green tea, inhibits the type iii secretion system (t3ss) of enteropathogenic and enterohemorrhagic escherichia coli (epec and ehec, respectively), salmonella enterica serovar typhimurium, and yersinia pseudotuberculosis. the inhibitory effect causes the inhibition of hemolysis, cell invasion, cell adhesion and apoptosis, which are functions of the type iii secretion device. in the case of epec, espb accumulates in the cells. rt-pcr showed th ... | 2017 | 28651361 |
| emergence of drug resistant bacteria at the hajj: a systematic review. | hajj is the annual mass gathering of muslims, and is a reservoir and potential source of bacterial transmission. the emergence of bacterial transmission, including multi-drug resistance (mdr) bacteria, during hajj has not been systematically assessed. | 2017 | 28652197 |
| stand-alone eal domain proteins form a distinct subclass of eal proteins involved in regulation of cell motility and biofilm formation in enterobacteria. | the second messenger cyclic dimeric gmp (c-di-gmp) is almost ubiquitous among bacteria as are the c-di-gmp turnover proteins, which mediate the transition between motility and sessility. eal domain proteins have been characterized as c-di-gmp-specific phosphodiesterases. while most eal domain proteins contain additional, usually n-terminal, domains, there is a distinct family of proteins with stand-alone eal domains, exemplified by salmonella enterica serovar typhimurium proteins stm3611 (yhjh/p ... | 2017 | 28652301 |
| hepcidin-(in)dependent mechanisms of iron metabolism regulation during infection by listeria and salmonella. | during bacterial infection, the pathogenic agent and the host battle for iron, due to its importance for fundamental cellular processes. however, iron redistribution and sequestration during infection can culminate in anemia. although hepcidin has been recognized as the key regulator of iron metabolism, in some infections its levels remain unaffected, suggesting the involvement of other players in iron metabolism deregulation. in this work, we use a mouse model to elucidate the main cellular and ... | 2017 | 28652306 |
| host and pathogen copper-transporting p-type atpases function antagonistically during salmonella infection. | copper is an essential yet potentially toxic trace element that is required by all aerobic organisms. a key regulator of copper homeostasis in mammalian cells is the copper-transporting p-type atpase atp7a, which mediates copper transport from the cytoplasm into the secretory pathway, as well as copper export across the plasma membrane. previous studies have shown that atp7a-dependent copper transport is required for killing phagocytosed escherichia coli in a cultured macrophage cell line. in th ... | 2017 | 28652309 |
| novel plasmid-mediated colistin resistance gene mcr-3 in escherichia coli. | the mobile colistin resistance gene mcr-1 has attracted global attention, as it heralds the breach of polymyxins, one of the last-resort antibiotics for the treatment of severe clinical infections caused by multidrug-resistant gram-negative bacteria. to date, six slightly different variants of mcr-1, and a second mobile colistin resistance gene, mcr-2, have been reported or annotated in the genbank database. here, we characterized a third mobile colistin resistance gene, mcr-3 the gene coexisted ... | 2017 | 28655818 |
| bacterial autophagy: offense and defense at the host-pathogen interface. | autophagy is a fundamental cellular process used for the turnover and recycling of cytosolic components and damaged organelles. originally characterized as a response to cellular stress, it now is well established that autophagy also is used as a defensive mechanism to combat the infection of host cells by intracellular pathogens. however, although this defensive strategy does limit the proliferation of most pathogens within their host cells, successful pathogens have evolved countermeasures tha ... | 2017 | 28660242 |
| complete genome sequences of two salmonella enterica subsp. enterica serovar enteritidis strains isolated from egg products in the united states. | egg-associated salmonellosis is an important public health problem in many countries. here, we report the genome sequences, including plasmids, of two strains of salmonella enterica subsp. enterica serovar enteritidis isolated from egg products in 2012 and 2013 in the united states. this will provide more information and insight into the research about egg-associated salmonellosis. | 2017 | 28663302 |
| sopb-mediated recruitment of snx18 facilitates salmonella typhimurium internalization by the host cell. | to invade epithelial cells, salmonella enterica serovar typhimurium (s. typhimurium) induces macropinocytosis through the action of virulence proteins delivered across the host cell membrane via a type iii secretion system. we show that after docking at the plasma membrane s. typhimurium triggers rapid recruitment of cytosolic snx18, a sh3-px-bar domain sorting nexin protein, to the bacteria-induced membrane ruffles and to the nascent salmonella-containing vacuole. snx18 recruitment required the ... | 2017 | 28664153 |
| microbial community profiling of fresh basil and pitfalls in taxonomic assignment of enterobacterial pathogenic species based upon 16s rrna amplicon sequencing. | application of 16s rrna (gene) amplicon sequencing on food samples is increasingly applied for assessing microbial diversity but may as unintended advantage also enable simultaneous detection of any human pathogens without a priori definition. in the present study high-throughput next-generation sequencing (ngs) of the v1-v2-v3 regions of the 16s rrna gene was applied to identify the bacteria present on fresh basil leaves. however, results were strongly impacted by variations in the bioinformati ... | 2017 | 28666129 |
| toxicity of coal fly ash (cfa) and toxicological response of switchgrass in mycorrhiza-mediated cfa-soil admixtures. | increasing support for the use of coal fly ash (cfa) in agriculture has necessitated a better understanding of the effects of the cfa in various cropping schemes. experiments were conducted to assess mutagenic response of a mutant strain of salmonella enterica serovar typhimurium (ta100) to varying concentrations of cfa-water extracts, determine oxidative stress in switchgrass (panicum virgatum l.) at varying levels of cfa-soil admixtures, and evaluate mycorrhiza-mediated modulation of oxidative ... | 2017 | 28666217 |
| spv locus aggravates salmonella infection of zebrafish adult by inducing th1/th2 shift to th2 polarization. | salmonella enterica serovar typhimurium (s. typhimurium) are facultative intracellular enteric pathogens causing disease with a broad range of hosts. it was known that th1-type cytokines such as ifn-γ, il-12, and tnf-α etc. could induce protective immunity against intracellular pathogens, while th2-type cytokines such as il-4, il-10, and il-13 etc. are proved to help pathogens survive inside hosts and cause severe infection. one of the critical virulence factor attributes to the pathogenesis of ... | 2017 | 28666864 |
| differences in expression of genes in the myd88 and trif signalling pathways and methylation of tlr4 and trif in tibetan chickens and daheng s03 chickens infected with salmonella enterica serovar enteritidis. | salmonella enterica serovar (s. enteritidis) is a pathogenic bacterium that can cause symptoms of food poisoning, leading to death of poultry, resulting in serious economic losses. the myd88 and trif signalling pathways play important roles in activating innate and adaptive immunity in chickens infected with s. enteritidis. the objective of the present study was to characterize in vivo mrna expressions, protein levels and methylation levels of genes in the above two pathways in both tibetan chic ... | 2017 | 28669384 |
| role of sapa and yfga in susceptibility to antibody-mediated complement-dependent killing and virulence of salmonella enterica serovar typhimurium. | the st313 pathovar of salmonella enterica serovar typhimurium contributes to a high burden of invasive disease among african infants and hiv-infected adults. it is characterized by genome degradation (loss of coding capacity) and has increased resistance to antibody-dependent complement-mediated killing compared with enterocolitis-causing strains of s typhimurium. vaccination is an attractive disease-prevention strategy, and leading candidates focus on the induction of bactericidal antibodies. a ... | 2017 | 28674031 |
| the strataa study protocol: a programme to assess the burden of enteric fever in bangladesh, malawi and nepal using prospective population census, passive surveillance, serological studies and healthcare utilisation surveys. | invasive infections caused by salmonella enterica serovar typhi and paratyphi a are estimated to account for 12-27 million febrile illness episodes worldwide annually. determining the true burden of typhoidal salmonellae infections is hindered by lack of population-based studies and adequate laboratory diagnostics.the strategic typhoid alliance across africa and asia study takes a systematic approach to measuring the age-stratified burden of clinical and subclinical disease caused by typhoidal s ... | 2017 | 28674145 |
| molecular subtyping of salmonella enterica serovar typhi by pulsed-field gel electrophoresis and multiple-locus variable-number tandem-repeat analysis in india: their association with antimicrobial resistance profiles. | molecular subtyping, and recently dna sequencing based methods, which are commonly used for discriminating salmonella enterica serovar typhi (s. typhi) isolates, leads to improved molecular epidemiological investigations for prevention and control of typhoid fever. we included s. typhi blood isolates (n=66) from india during 2007-14 for molecular subtyping by pulsed-field gel electrophoresis (pfge) and multiple-locus variable-number tandem-repeat (vntr) analysis (mlva) in association with antibi ... | 2017 | 28674312 |
| super-cationic carbon quantum dots synthesized from spermidine as an eye drop formulation for topical treatment of bacterial keratitis. | we have developed a one-step method to synthesize carbon quantum dots (cqdpas) from biogenic polyamines (pas) as an antibacterial agent for topical treatment of bacterial keratitis (bk). cqds synthesized by direct pyrolysis of spermidine (spd) powder through a simple dry heating treatment exhibit a solubility and yield much higher than those from putrescine and spermine. we demonstrate that cqds obtained from spds (cqdspds) possess effective antibacterial activities against non-multidrug-resista ... | 2017 | 28677399 |
| serotype distribution, antimicrobial resistance, and class 1 integrons profiles of salmonella from animals in slaughterhouses in shandong province, china. | the current study aimed to analyze the prevalence and characterization of salmonella enterica isolated from animals in slaughterhouses before slaughter. a total of 143 non-duplicate salmonella were recovered from 1,000 fresh fecal swabs collected from four major pig slaughterhouses (49/600, 8.2%) and four major chicken slaughterhouses (94/400, 23.5%) between march and july 2016. among salmonella isolates from pigs, the predominant serovars were salmonella rissen (28/49, 57.1%) and typhimurium (1 ... | 2017 | 28680418 |
| combination of antimicrobials and essential oils as an alternative for the control of salmonella enterica multiresistant strains related to foodborne disease. | due to the increase in bacterial resistance to antimicrobials (ambs) commonly used in veterinary and human medicine, the new strategies for controlling zoonoses focus on the study of natural products with demonstrated amb activity, such as essential oils (eos). the aim of this study was to evaluate the in vitro effect of the combination of enrofloxacin (enr), ceftiofur (cef), and trimethoprim-sulfamethoxazole (sxt) with cinnamon, clove, oregano, and red thyme eos against multiple drug-resistant ... | 2017 | 28683217 |
| genomic characterization of a large plasmid containing a bla ndm-1 gene carried on salmonella enterica serovar indiana c629 isolate from china. | the bla ndm-1 gene in salmonella species is mostly reported in clinical cases, but is rarely isolated from red and white meat in china. | 2017 | 28687066 |
| diversity of antimicrobial resistance phenotypes in salmonella isolated from commercial poultry farms. | salmonella remains the leading cause of foodborne illness in the united states, and the dissemination of drug-resistant salmonellae through the food chain has important implications for treatment failure of salmonellosis. we investigated the ecology of salmonella in integrated broiler production in order to understand the flow of antibiotic susceptible and resistant strains within this system. data were analyzed from a retrospective study focused on antimicrobial resistant salmonella recovered f ... | 2017 | 28691011 |
| purification and characterization of an atpase gsia from salmonella enterica. | the coding sequence of salmonella enterica gsia was cloned and expressed in e. coli. the protein was purified and atpase activity was characterized by nadh oxidation method. gsia exhibited optimum activity at 30°c and at ph 8 in tris/hcl buffer. gsia protein was stable at 20°c. 66% and 44% activity remained after incubation at 30°c and 40°c for 30 min. ph 7 and ph 9 incubation would obviously reduce the atpase activity. in vivo functionality of gsia was determined by constructing gene deletion s ... | 2017 | 28691022 |
| role of the inner-membrane histidine kinase rcsc and outer-membrane lipoprotein rcsf in the activation of the rcs phosphorelay signal transduction system in escherichia coli. | the rcs phosphorelay signal transduction system of escherichia coli controls genes for capsule production and many other envelope-related functions and is implicated in biofilm formation. the outer-membrane lipoprotein rcsf is an essential component of the rcs system. mislocalization of rcsf to the periplasm or the cytoplasmic membrane leads to high activation of the rcs system, suggesting that rcsf functions by interacting with the cytoplasmic membrane component(s) of the system in activating t ... | 2017 | 28691662 |
| population-based incidence, seasonality and serotype distribution of invasive salmonellosis among children in nanoro, rural burkina faso. | bloodstream infections (bsi) caused by salmonella typhi and invasive non-typhoidal salmonella (ints) frequently affect children living in rural sub-saharan africa but data about incidence and serotype distribution are rare. | 2017 | 28692655 |
| relationship of triamine-biocide tolerance of salmonella enterica serovar senftenberg to antimicrobial susceptibility, serum resistance and outer membrane proteins. | a new emerging phenomenon is the association between the incorrect use of biocides in the process of disinfection in farms and the emergence of cross-resistance in salmonella populations. adaptation of the microorganisms to the sub-inhibitory concentrations of the disinfectants is not clear, but may result in an increase of sensitivity or resistance to antibiotics, depending on the biocide used and the challenged salmonella serovar. exposure of five salmonella enterica subsp. enterica serovar se ... | 2017 | 28696348 |
| development of a novel, rapid multiplex polymerase chain reaction assay for the detection and differentiation of salmonella enterica serovars enteritidis and typhimurium using ultra-fast convection polymerase chain reaction. | salmonella enterica serovars enteritidis and typhimurium are the most common causative agents of human nontyphoidal salmonellosis. the rapid detection and timely treatment of salmonellosis are important to increase the curative ratio and prevent spreading of the disease. in this study, we developed a rapid multiplex convection polymerase chain reaction (pcr) method to detect salmonella spp. and differentiate salmonella enteritidis and salmonella typhimurium. we used the inva gene for salmonella ... | 2017 | 28696782 |
| salmonella enterica serovar typhimurium st34 co-expressing blandm-5 and blactx-m-55 isolated in china. | 2017 | 28698668 | |
| quinolones and fluroquinolones metabolism and resistance in salmonella typhimurium: a mini review. | salmonella enterica serovars causes substantial morbidity and mortality worldwide. more than 2500 different serotypes of salmonella were identified to date. in particular, salmonella enterica serovar typhimurium is the most important serotype of salmonella transmitted from animals to humans in most parts of the world. they are associated with an estimated 1 million deaths annually. quinolones are an important class of broad-spectrum antimicrobials has been utilized in the treatment option for ov ... | 2017 | 28699507 |
| draft genome sequences of 256 salmonella enterica subsp. enterica serovar enteritidis strains isolated from humans, food, chickens, and farm environments in brazil. | salmonella enterica subsp. enterica serovar enteritidis emerged in the late 1980s as the most isolated salmonella serovar worldwide. here, we report the draft genomes of 256 s enteritidis strains isolated from humans, food, chickens, and farm environments in brazil. these draft genomes will help enhance our understanding of this serovar in brazil. | 2017 | 28705959 |
| salmonella typhimurium infection in total knee arthroplasty: a case report with review of literature. | salmonella enterica serotype typhimurium is a rare cause of prosthetic joint infection (pji). the recognized predisposing risk factors for salmonella septic arthritis include diabetes mellitus, renal failure, human immunodeficiency virus infection and chronic corticosteroid use. we describe a case of pji of the knee in a 74-year-old lady who was on antitubercular treatment. the patient presented with discharging sinus and raised inflammatory markers. she was successfully treated by the removal o ... | 2017 | 28706395 |
| phenotypic characteristics and genetic diversity of salmonella enterica serotype derby isolated from human patients and foods of animal origin. | salmonella enterica serotype derby is among the three most common serotypes of nontyphoidal salmonella isolated from patients with diarrhea in china. in this study, 133 salmonella derby isolates from human patients (n = 74) and foods of animal origin (n = 59) in shanghai, china, between september 2013 and december 2014, were selected to study its phenotypic characteristics and genetic diversity. the isolates were subjected to antimicrobial susceptibility testing, plasmid replicon typing, virulen ... | 2017 | 28708426 |
| selection of potential probiotic lactobacillus with inhibitory activity against salmonella and fecal coliform bacteria. | three hundred and sixty presumptive lactic acid bacteria (lab) isolated from pregnant sows, newborn, suckling, and weaned piglets were preliminarily screened for anti-salmonella activity. fifty-eight isolates consisting of lactobacillus reuteri (n = 32), lactobacillus salivarius (n = 10), lactobacillus mucosae (n = 8), lactobacillus johnsonii (n = 5), and lactobacillus crispatus (n = 3) were selected and further characterized for probiotic properties including production of antimicrobial substan ... | 2017 | 28712023 |
| detection of eight foodborne bacterial pathogens by oligonucleotide array hybridization. | simultaneous and rapid detection of multiple foodborne bacterial pathogens is important for the prevention of foodborne illnesses. | 2017 | 28713514 |
| intestinal epithelial ecto-5' -nucleotidase (cd73) regulates intestinal colonization and infection by nontyphoidal salmonella. | ecto-5' -nucleotidase (cd73) is expressed abundantly on the apical surface of intestinal epithelial cells (iec) and functions as the terminal enzyme in the generation of extracellular adenosine. previous work has demonstrated that adenosine signaling in iecs results in a number of tissue-protective effects during inflammation; however, a rationale for its apical expression has been lacking. we hypothesized that the highly polarized expression of cd73 is indicative of an important role for extrac ... | 2017 | 28717030 |
| multidrug-resistant salmonellae isolated in japanese quails reared in abeokuta, nigeria. | salmonellosis is a major bacterial disease causing huge economic losses in the poultry industry worldwide. this study was carried out to determine the period prevalence and antimicrobial susceptibility of salmonella enterica in japanese quails in abeokuta, nigeria. four hundred cloacal swabs of quail birds were collected from 4 locations within abeokuta. salmonella was isolated from the samples using conventional methods for selective isolation of salmonella and biochemical identification. isola ... | 2017 | 28717851 |
| sequence-based identification of inositol monophosphatase-like histidinol-phosphate phosphatases (hisn) in corynebacterium glutamicum, actinobacteria, and beyond. | the eighth step of l-histidine biosynthesis is carried out by an enzyme called histidinol-phosphate phosphatase (holpase). three unrelated holpase families are known so far. two of them are well studied: had-type holpases known from gammaproteobacteria like escherichia coli or salmonella enterica and php-type holpases known from yeast and firmicutes like bacillus subtilis. however, the third family of holpases, the inositol monophosphatase (impase)-like holpases, present in actinobacteria like c ... | 2017 | 28720084 |
| lack of acrb efflux function confers loss of virulence on salmonella enterica serovar typhimurium. | acrab-tolc is the paradigm resistance-nodulation-division (rnd) multidrug resistance efflux system in gram-negative bacteria, with acrb being the pump protein in this complex. we constructed a nonfunctional acrb mutant by replacing d408, a highly conserved residue essential for proton translocation. western blotting confirmed that the acrb d408a mutant had the same native level of expression of acrb as the parental strain. the mutant had no growth deficiencies in rich or minimal medium. however, ... | 2017 | 28720734 |
| real-time optotracing of curli and cellulose in live salmonella biofilms using luminescent oligothiophenes. | extracellular matrix (ecm) is the protein- and polysaccharide-rich backbone of bacterial biofilms that provides a defensive barrier in clinical, environmental and industrial settings. understanding the dynamics of biofilm formation in native environments has been hindered by a lack of research tools. here we report a method for simultaneous, real-time, in situ detection and differentiation of the salmonella ecm components curli and cellulose, using non-toxic, luminescent conjugated oligothiophen ... | 2016 | 28721253 |
| development and evaluation of a multiple-locus variable-number tandem-repeats analysis assay for subtyping salmonella typhi strains from sub-saharan africa. | molecular epidemiological investigations of the highly clonal salmonella enterica subspecies enterica serovar typhi (s. typhi) are important in outbreak detection and in tracking disease transmission. in this study, we developed and evaluated a multiple-locus variable-number tandem-repeats (vntr) analysis (mlva) assay for characterization of s. typhi isolates from sub-saharan africa. | 2017 | 28721851 |
| serum albumin and osmolality inhibit bdellovibrio bacteriovorus predation in human serum. | we evaluated the bactericidal activity of bdellovibrio bacteriovorus, strain hd100, within blood sera against bacterial strains commonly associated with bacteremic infections, including e. coli, klebsiella pneumoniae and salmonella enterica. tests show that b. bacteriovorus hd100 is not susceptible to serum complement or its bactericidal activity. after a two hour exposure to human sera, the prey populations decreased 15- to 7,300-fold due to the serum complement activity while, in contrast, the ... | 2017 | 28725056 |
| physiological fluid shear alters the virulence potential of invasive multidrug-resistant non-typhoidal salmonella typhimurium d23580. | salmonella enterica serovar typhimurium strains belonging to sequence type st313 are a major cause of fatal bacteremia among hiv-infected adults and children in sub-saharan africa. unlike "classical" non-typhoidal salmonella (nts), gastroenteritis is often absent during st313 infections and isolates are most commonly recovered from blood, rather than from stool. this is consistent with observations in animals, in which st313 strains displayed lower levels of intestinal colonization and higher re ... | 2016 | 28725732 |
| feed withdrawal and transportation effects on salmonella enterica levels in market-weight pigs. | feed withdrawal and transport commonly occur together in pigs. objectives of this study were to determine if these preslaughter stressors, feed withdrawal and transportation, affect the levels of , stress hormone concentrations, and immune functions in infected market pigs. a 2 × 2 factorial analysis of a randomized complete block design with feed withdrawal and transport as fixed effects was used. sixty market-weight pigs were individually inoculated with serovar typhimurium. the experiment was ... | 2017 | 28727113 |
| a cross-sectional seroepidemiological survey of typhoid fever in fiji. | fiji, an upper-middle income state in the pacific ocean, has experienced an increase in confirmed case notifications of enteric fever caused by salmonella enterica serovar typhi (s. typhi). to characterize the epidemiology of typhoid exposure, we conducted a cross-sectional sero-epidemiological survey measuring igg against the vi antigen of s. typhi to estimate the effect of age, ethnicity, and other variables on seroprevalence. epidemiologically relevant cut-off titres were established using a ... | 2017 | 28727726 |
| draft genome sequence of salmonella enterica subsp. enterica serovar infantis strain spe101, isolated from a chronic human infection. | we report a 4.99-mb draft genome sequence of salmonella enterica subsp. enterica serovar infantis strain spe101, isolated from feces of a 5-month-old breast-fed female showing diarrhea associated with severe dehydration and malnutrition. the infection prolonged for 6 months despite antibiotic treatment. | 2017 | 28729277 |
| rho protein: roles and mechanisms. | at the end of the multistep transcription process, the elongating rna polymerase (rnap) is dislodged from the dna template either at specific dna sequences, called the terminators, or by a nascent rna-dependent helicase, rho. in escherichia coli, about half of the transcription events are terminated by the rho protein. rho utilizes its rna-dependent atpase activities to translocate along the mrna and eventually dislodges the rnap via an unknown mechanism. the transcription elongation factor nusg ... | 2017 | 28731845 |
| influence of saccharomyces cerevisiae fermentation products, smartcare in milk replacer and original xpc in calf starter, on the performance and health of preweaned holstein calves challenged with salmonella enterica serotype typhimurium. | this study was designed to investigate the effects of supplementing smartcare (sc; diamond v, cedar rapids, ia) in milk replacer and original xpc (xpc; diamond v) in calf starter on performance and health of preweaned calves following an oral challenge with salmonella enterica. the study was performed in two 35-d periods with 30 holstein bull calves (2 ± 1 d of age) per period. in each period, calves were blocked by location in the barn and randomly assigned to treatments that included control, ... | 2017 | 28734601 |
| a recombinant protein of salmonella typhi induces humoral and cell-mediated immune responses including memory responses. | gram negative enteric bacteria, salmonella enterica serovar typhi (s. typhi), the etiological agent of typhoid fever is a major public health problem in developing countries. while a permanent solution to the problem would require improved sanitation, food and water hygiene, controlling the infection by vaccination is urgently required due to the emergence of multidrug resistant strains in multiple countries. the currently licensed vaccines are moderately efficacious with limited applicability, ... | 2017 | 28739115 |
| shape analysis of the htra rna thermometer from salmonella enterica. | rna thermometers regulate expression of prokaryotic genes involved in heat shock response or in virulence of pathogenic bacteria such as yersinia, neisseria, and salmonella they function through temperature-dependent conformational changes in the mrnas in which they reside. most rna thermometers are found in the 5'-untranslated region (utr) of the mrna, where they modulate availability of the ribosome-binding site. we have used shape (selective 2'-hydroxyl acylation analyzed by primer extension) ... | 2017 | 28739676 |
| the influence of ultrasonic treatment on the growth of the strains of salmonella enterica subs. typhimurium. | this study proposes the destruction of pathogenic bacteria with the use of ultrasound waves because the more commonly used thermal processing methods often result in lowering the nutritional value of food. the study presents the impact of ultrasound of 20, 40 and 100 khz frequencies and the power of 10.5 w/cm(2) on the growth of the strain of salmonella enterica subs. typhimurium. the tests were carried out both in chilled and non-chilled treatment mediums, with an average bacterial population > ... | 2017 | 28740277 |
| prophage integrase typing is a useful indicator of genomic diversity in salmonella enterica. | salmonella enterica is a bacterial species that is a major cause of illness in humans and food-producing animals. s. enterica exhibits considerable inter-serovar diversity, as evidenced by the large number of host adapted serovars that have been identified. the development of methods to assess genome diversity in s. enterica will help to further define the limits of diversity in this foodborne pathogen. thus, we evaluated a pcr assay, which targets prophage integrase genes, as a rapid method to ... | 2017 | 28740489 |
| salmonella enterica serovar-specific transcriptional reprogramming of infected cells. | despite their high degree of genomic similarity, different salmonella enterica serovars are often associated with very different clinical presentations. in humans, for example, the typhoidal s. enterica serovar typhi causes typhoid fever, a life-threatening systemic disease. in contrast, the non-typhoidal s. enterica serovar typhimurium causes self-limiting gastroenteritis. the molecular bases for these different clinical presentations are incompletely understood. the ability to re-program gene ... | 2017 | 28742135 |
| salmonella choleraesuis outer membrane vesicles: proteomics and immunogenicity. | salmonella enterica serotype choleraesuis (s. choleraesuis), gram-negative facultative intracellular pathogen is capable of inducing the cholera in pigs whose symptoms manifest as fever, depression, septicemia, arthritis, and diarrhea. infections with s. choleraesuis has resulted in great economic loss for the swine breeding operations. bacterial outer membrane vesicles (omvs) play an important role in pathogenicity and host-pathogen interaction. in this study, we purified omvs released by s. ch ... | 2017 | 28745825 |
| quinolone susceptibility and genetic characterization of salmonella enterica subsp. enterica isolated from pet turtles. | turtle-borne salmonella enterica owns significance as a leading cause in human salmonellosis. the current study aimed to determine the quinolone susceptibility and the genetic characteristics of 21 strains of s. enterica subsp. enterica isolated from pet turtles. susceptibility of four antimicrobials including nalidixic acid, ciprofloxacin, ofloxacin, and levofloxacin was examined in disk diffusion and mic tests where the majority of the isolates were susceptible to all tested quinolones. in gen ... | 2017 | 28747968 |
| antimicrobial property of lemongrass (cymbopogon citratus) oil against pathogenic bacteria isolated from pet turtles. | the usage of essential oils as antimicrobial agents is gaining attention. besides, pet turtles were known to harbor a range of pathogenic bacteria while the turtle keeping is a growing trend worldwide.the current study examined the antimicrobial activity of lemon grass oil (lgo) against seven species of gram negative bacteria namely; aeromonas hydrophila, a. caviae, citrobacter freundii, salmonella enterica, edwardsiella tarda, pseudomonas aeruginosa, and proteus mirabilis isolated from three po ... | 2017 | 28747972 |
| genome target evaluator (gtevaluator): a workflow exploiting genome dataset to measure the sensitivity and specificity of genetic markers. | most of the bacterial typing methods used to discriminate isolates in medical or food safety microbiology are based on genetic markers used as targets in pcr or hybridization experiments. these dna typing methods are important tools for studying prevalence and epidemiology, for conducting surveillance, investigations and control of biological hazard sources. in that perspective, it is crucial to insure that the chosen genetic markers have the greatest specificity and sensitivity. the wealth of w ... | 2017 | 28750049 |
| whole-genome shotgun sequences of salmonella enterica serovar typhimurium lilleengen type strains lt1, lt18, lt19, lt20, lt21, and lt22. | the lilleengen type (lt) collection of salmonella enterica serovar typhimurium strains has served the scientific community as a group of model organisms for basic genetic and biochemical pathway research. here, we report the whole-genome shotgun sequences of salmonella enterica serovar typhimurium strains lt1, lt18, lt19, lt20, lt21, and lt22. | 2017 | 28751402 |
| salmonella enterica serovar infantis from food and human infections, switzerland, 2010-2015: poultry-related multidrug resistant clones and an emerging esbl producing clonal lineage. | objectives: the aim of this study was to characterize a collection of 520 salmonella enterica serovar infantis strains isolated from food (poultry meat), human infections and environmental sources from the years 2010, 2013 and 2015 in switzerland. methods: we performed antimicrobial susceptibility testing and pulsed-field gel electrophoresis (pfge) analysis on all 520 s. infantis isolates, and whole genome sequencing (wgs) on 32 selected isolates. results: the majority (74.8%) of the isolates wa ... | 2017 | 28751886 |
| seabirds (laridae) as a source of campylobacter spp., salmonella spp. and antimicrobial resistance in south africa. | zoonotic thermophilic campylobacter and nontyphoidal salmonella enterica are a major cause of foodborne human gastroenteritis worldwide. there is little information about reservoirs of these zoonotic agents in africa. thus, chicks of kelp gulls (larus dominicanus, n=129) and greater crested terns (thalasseus bergii, n=100) were studied at five colonies on the western cape coast (south africa) during summer 2013/2014. campylobacter spp. occurrence was 14.0% (ci95% : 9.9-19.3), with c. jejuni the ... | 2017 | 28752962 |
| repression of salmonella host cell invasion by aromatic small molecules from the human fecal metabolome. | the human microbiome is a collection of microorganisms that inhabit every surface of the body that is exposed to the environment, generally coexisting peacefully with their host. these microbes have important functions such as the production of vitamins, maturation of the immune system and protection against pathogens. we have previously shown that a small-molecule extract from the human fecal microbiome has a strong repressive effect on salmonella enterica serovar typhimurium host cell invasion ... | 2017 | 28754707 |
| cyclohexene-fused 1,3-oxazines with selective antibacterial and antiparasitic action and low cytotoxic effects. | oxazine derivatives, a class of heterocyclic compounds, exhibit a variety of biological properties, such as anticonvulsant and antitumor activities. in this study, we evaluated the effect of two cyclohexene-fused 1,3-oxazines (cis‑1-benzyl-n-phenyl-1,4,4a,5,8,8a-hexahydro-3,1-benzoxazin-2-imine (1) and trans‑n-phenyl-1,4,4a,5,8,8a-hexahydro-3,1-benzoxazin-2-imine (2)) in cultures of bacillus cereus, enterococcus faecalis, escherichia coli, klebsiella pneumoniae, salmonella enterica, serratia mar ... | 2017 | 28755871 |
| ground water as the source of an outbreak of salmonella enteritidis. | in september 2014, an outbreak of gastroenteritis was reported to the public health institute of šibenik and knin county in croatia. the outbreak occurred in the county center of šibenik, a town with 50,000 inhabitants, and it lasted for 12days. an epidemiological investigation suggested a nearby water spring as the source of the outbreak. due to the temporary closure of the public water supply system, the inhabitants started to use untreated water from a nearby spring. microbiological analysis ... | 2017 | 28756827 |
| drug resistance pattern in the recent isolates of salmonella typhi with special reference to cephalosporins and azithromycin in the gangetic plain. | typhoid fever is an endemic disease in india against which many antibiotics are available. in the recent times, emerging resistance to traditional antibiotics, such as ampicillin, chloramphenicol and trimethoprim/sulfamethoxazole, azithro-mycin and third generation cephalosporins are being reported and increasingly being used in the treatment of invasive salmonella infections. however, the latter two drugs have been reported with occasional clinical failures. currently, we do not have data regar ... | 2017 | 28764168 |
| a randomized trial to assess the effect of fluoroquinolone metaphylaxis on the fecal prevalence and quinolone susceptibilities of salmonella and campylobacter in feedlot cattle. | the study objective was to determine effects of fluoroquinolone metaphylaxis on fecal prevalence of salmonella and campylobacter and fecal prevalence of quinolone-resistant salmonella and campylobacter in feedlot cattle. on day 0, cattle (n = 288) at risk for bovine respiratory disease (brd) were randomly assigned to either a nontreated control pen (12 pens) or a fluoroquinolone-treated (enrofloxacin; baytril(®) 100) pen (12 pens). rectal fecal samples were collected from cattle on days 0, 7, 14 ... | 2017 | 28768141 |
| comparative genomic analysis and characterization of incompatibility group fib plasmid encoded virulence factors of salmonella enterica isolated from food sources. | the degree to which the chromosomal mediated iron acquisition system contributes to virulence of many bacterial pathogens is well defined. however, the functional roles of plasmid encoded iron acquisition systems, specifically sit and aerobactin, have yet to be determined for salmonella spp. in a recent study, salmonella enterica strains isolated from different food sources were sequenced on the illumina miseq platform and found to harbor the incompatibility group (inc) fib plasmid. in this stud ... | 2017 | 28768482 |
| the transient multidrug resistance phenotype of salmonella enterica swarming cells is abolished by sub-inhibitory concentrations of antimicrobial compounds. | swarming motility is the rapid and coordinated multicellular migration of bacteria across a moist surface. during swarming, bacterial cells exhibit increased resistance to multiple antibiotics, a phenomenon described as adaptive or transient resistance. in this study, we demonstrate that sub-inhibitory concentrations of cefotaxime, ciprofloxacin, trimethoprim, or chloramphenicol, but not that of amikacin, colistin, kanamycin or tetracycline, impair salmonella enterica swarming. chloramphenicol-t ... | 2017 | 28769915 |
| oral administration of lactobacillus rhamnosus gg ameliorates salmonella infantis-induced inflammation in a pig model via activation of the il-22bp/il-22/stat3 pathway. | the high rate of salmonella enterica serovar infantis (s. infantis) infection poses significant risk for the development of non-typhoidal salmonella gastroenteritis. however, efficient strategies to prevent or treat the infection remain elusive. here, we explored the effect of the probiotic lactobacillus rhamnosus gg (lgg) administration in preventing s. infantis infection in a pig model. probiotic lgg (1.0 × 10(10) cfu/day) was orally administered to newly weaned piglets for 1 week before s. in ... | 2017 | 28770173 |
| validation of thermal lethality against salmonella enterica in poultry offal during rendering. | recent outbreaks of human disease following contact with companion animal foods cross-contaminated with enteric pathogens, such as salmonella enterica, have resulted in increased concern regarding the microbiological safety of animal foods. additionally, the u.s. food and drug administration food safety modernization act and its implementing rules have stipulated the implementation of current good manufacturing practices and food safety preventive controls for livestock and companion animal food ... | 2017 | 28771040 |
| assembly and stoichiometry of the core structure of the bacterial flagellar type iii export gate complex. | the bacterial flagellar type iii export apparatus, which is required for flagellar assembly beyond the cell membranes, consists of a transmembrane export gate complex and a cytoplasmic atpase complex. flha, flhb, flip, fliq, and flir form the gate complex inside the basal body ms ring, although flio is required for efficient export gate formation in salmonella enterica. however, it remains unknown how they form the gate complex. here we report that flip forms a homohexameric ring with a diameter ... | 2017 | 28771466 |
| draft genome sequences of salmonella enterica serovar typhimurium lt2 with deleted chitinases that are emerging virulence factors. | chitinases are glycosyl hydrolases that catalyze the hydrolysis of the β-1,4 linkages in complex carbohydrates and those that contain glcnac. these enzymes are considered emerging virulence factors during infection because the host glycan changes. this is the release of four single chitinase deletion mutants in salmonella enterica serovar typhimurium lt2. | 2017 | 28774970 |
| a third family of allelic hsd genes in salmonella enterica: sequence comparisons with related proteins identify conserved regions implicated in restriction of dna. | salmonella enterica serovar blegdam has a restriction and modification system encoded by genes linked to serb. we have cloned these genes, putative alleles of the hsd locus of escherichia coli k-12, and confirmed by the sequence similarities of flanking dna that the hsd genes of s. enterica serovar blegdam have the same chromosomal location as those of e. coli k-12 and salmonella enterica serovar typhimurium lt2. there is, however, no obvious similarity in their nucleotide sequences, and while t ... | 1996 | 28777503 |
| plant-mediated restriction of salmonella enterica on tomato and spinach leaves colonized with pseudomonas plant growth-promoting rhizobacteria. | reducing salmonella enterica association with plants during crop production could reduce risks of fresh produce-borne salmonellosis. plant growth-promoting rhizobacteria (pgpr) colonizing plant roots are capable of promoting plant growth and boosting resistance to disease, but the effects of pgpr on human pathogen-plant associations are not known. two root-colonizing pseudomonas strains s2 and s4 were investigated in spinach, lettuce and tomato for their plant growth-promoting properties and the ... | 2017 | 28778009 |
| the invasome of salmonella dublin as revealed by whole genome sequencing. | salmonella enterica serovar dublin is a zoonotic infection that can be transmitted from cattle to humans through consumption of contaminated milk and milk products. outbreaks of human infections by s. dublin have been reported in several countries including high-income countries. a high proportion of s. dublin cases in humans are associated with invasive disease and systemic illness. the genetic basis of virulence in s. dublin is not well characterized. | 2017 | 28778189 |
| potential of 2, 2'-dipyridyl diselane as an adjunct to antibiotics to manage cadmium-induced antibiotic resistance in salmonella enterica serovar typhi ty2 strain. | one of the reasons for increased antibiotic resistance in salmonella enterica serovar typhi ty2 is the influx of heavy metal ions in the sewage, from where the infection is transmitted. therefore, curbing these selective agents could be one of the strategies to manage the emergence of multidrug resistance in the pathogen. as observed in our earlier study, the present study also confirmed the links between cadmium accumulation and antibiotic resistance in salmonella. therefore, the potential of a ... | 2017 | 28779338 |
| purulent pericarditis with salmonella enterica subspecies arizona in a patient with type 2 diabetes mellitus. | purulent pericarditis is a life-threatening disorder, even in the modern antibiotic era. although diabetes mellitus is known to be associated with an increased risk of multiple types of infections, purulent pericarditis is extremely rare. we herein report an unusual case of pericarditis caused by salmonella enterica subspecies arizona that was not associated with any evident underlying immunosuppressive disorder apart from uncontrolled type 2 diabetes mellitus. because a pet snake was suspected ... | 2017 | 28781305 |
| determination of optimum sanitizer levels for prevention of salmonella cross-contamination of mature round tomatoes in a laboratory model flume system. | salmonella has been reported to be involved in several foodborne illness outbreaks, many of which resulted from consumption of raw tomatoes. this research aimed to optimize and evaluate the concentration of free chlorine (hypochlorous acid [hocl]) used as a sanitizer to prevent cross-contamination of tomatoes inoculated with a cocktail of five rifampin-resistant salmonella enterica serovars in a laboratory-based model flume system. organic load, prepared using sterilized scotts premium topsoil, ... | 2017 | 28782995 |
| study of antimicrobial resistance and physiological biomarkers with special reference to salmonellosis in diarrheic foals in punjab, pakistan. | antimicrobial resistance results in selective colonization in animals. in the present study, 447 diarrheic foals (235 horse foals, 165 donkey foals and 47 mule foal) were selected from lahore and sahiwal districts of punjab, pakistan. fresh fecal and blood samples from diarrheic foals were collected for isolation and confirmation of salmonella polymerase chain reaction. results revealed that 50 (11.25%) foals (horse n=29, donkey n=12 and mule n=9) were positive. fifty salmonella enterica isolate ... | 2017 | 28784420 |
| prevalence and trends in the antimicrobial susceptibility pattern of salmonella enterica serovars typhi and paratyphi a among children in a pediatric tertiary care hospital in south india over a period of ten years: a retrospective study. | the aim of this study was to determine the prevalence and trends in the antimicrobial resistance of typhoidal salmonellae in children and adolescents at a pediatric tertiary care hospital in south india. typhoidal salmonellae were isolated from 483 of the 77,713 blood cultures received during the ten-year study period (2007-2016). isolates were speciated by conventional biochemical reactions and serotyping. antimicrobial susceptibility testing was performed and interpreted according to the briti ... | 2017 | 28785823 |
| insights from protein-protein interaction studies on bacterial pathogenesis. | the threat bacterial pathogens pose to human health is increasing with the number and distribution of antibiotic-resistant bacteria, while the rate of discovery of new antimicrobials dwindles. proteomics is playing key roles in understanding the molecular mechanisms of bacterial pathogenesis, and in identifying disease outcome determinants. the physical associations identified by proteomics can provide the means to develop pathogen-specific treatment methods that reduce the spread of antibiotic ... | 2017 | 28786313 |
| what the sif is happening-the role of intracellular salmonella-induced filaments. | a common strategy among intracellular bacterial pathogens is to enter into a vacuolar environment upon host cell invasion. one such pathogen, salmonella enterica, resides within the salmonella-containing vacuole (scv) inside epithelial cells and macrophages. salmonella hijacks the host endosomal system to establish this unique intracellular replicative niche, forming a highly complex and dynamic network of salmonella-induced filaments (sifs). sifs radiate outwards from the scv upon onset of bact ... | 2017 | 28791257 |
| construction of an inactivated typhoid vaccine candidate expressing escherichia coli heat-labile enterotoxin b subunit and evaluation of its immunogenicity in a murine model. | typhoid fever caused by salmonella enterica serovar typhi has contributed to the global public health burden, particularly in developing countries. in this study, an s. typhi ghost was developed and its capacity as a vaccine candidate against typhoid fever was assessed. | 2017 | 28792376 |
| defensin-neurotoxin dyad in a basally-branching metazoan sea anemone. | recent studies suggest that vertebrate and invertebrate defensins have evolved from two independent ancestors, and that both defensins could share origins with animal toxins. here, we purified novel sea anemone neurotoxin (bds)-like antimicrobial peptides (amps)-crassicorin-i and its putative homolog (crassicorin-ii)-from the pharynx extract of an anthozoan sea anemone (urticina crassicornis). based on structural analyses and cdna cloning, mature crassicorin-i represents a cationic amp likely ge ... | 2017 | 28796463 |
| 6s rna is involved in acid resistance and invasion of epithelial cells in salmonella enterica serovar typhimurium. | acid is an important environmental condition encountered frequently by salmonella enterica serovar typhimurium during its pathogenesis, but the role of small-noncoding rnas (srnas) in response to acid stress is poorly understood. | 2017 | 28796533 |
| transcriptome and proteome analysis of salmonella enterica serovar typhimurium systemic infection of wild type and immune-deficient mice. | salmonella enterica are a threat to public health. current vaccines are not fully effective. the ability to grow in infected tissues within phagocytes is required for s. enterica virulence in systemic disease. as the infection progresses the bacteria are exposed to a complex host immune response. consequently, in order to continue growing in the tissues, s. enterica requires the coordinated regulation of fitness genes. bacterial gene regulation has so far been investigated largely using exposure ... | 2017 | 28796780 |
| novel plasmid-mediated colistin resistance mcr-4 gene in salmonella and escherichia coli, italy 2013, spain and belgium, 2015 to 2016. | a novel mcr colistin resistance gene was identified in a strain of salmonella enterica, monophasic variant of serovar typhimurium (4,5,12:i:- ), isolated from a pig at slaughter in italy in 2013, and in escherichia coli strains collected during routine diagnostic of post-weaning diarrhoea in pigs from spain and belgium in 2015 and 2016. immediate implementation of mcr-screening including this novel gene variant is required for salmonella and e. coli from humans and food-producing animals in euro ... | 2017 | 28797329 |
| salmonella spi-2 type iii secretion system effectors: molecular mechanisms and physiological consequences. | serovars of salmonella enterica cause both gastrointestinal and systemic diseases in a broad range of mammalian hosts, including humans. salmonella virulence depends in part on its pathogenicity island 2 type iii secretion system (spi-2 t3ss), which is required to translocate at least 28 effector proteins from vacuolar-resident bacteria into host cells. comparative genomic analysis reveals that all serovars encode a subset of "core" effectors, suggesting that they are critical for virulence in d ... | 2017 | 28799907 |
| estimation of the probability of bacterial population survival: development of a probability model to describe the variability in time to inactivation of salmonella enterica. | despite the development of numerous predictive microbial inactivation models, a model focusing on the variability in time to inactivation for a bacterial population has not been developed. additionally, an appropriate estimation of the risk of there being any remaining bacterial survivors in foods after the application of an inactivation treatment has not yet been established. here, gamma distribution, as a representative probability distribution, was used to estimate the variability in time to ... | 2017 | 28800819 |
| functional analysis of genes encoded by the locus of heat resistance (lhr) in escherichia coli. | the locus of heat resistance (lhr) is a 15 - 19 kb genomic island conferring exceptional heat resistance to organisms in the family enterobacteriaceae including pathogenic strains of salmonella enterica and escherichia coli the complement of lhr-encoded genes that is necessary for heat resistance and the stress- or growth-phase induced expression of lhr-encoded genes are unknown. this study determined the contribution of the 7 lhr-encoded genes yfdx1gi, yfdx2, hdedgi, orf11, trxgi, kefb, and psi ... | 2017 | 28802266 |
| association between wetland presence and incidence of salmonella enterica serotype javiana infections in selected us sites, 2005-2011. | salmonella causes an estimated 1·2 million illnesses annually in the usa. salmonella enterica serotype javiana (serotype javiana) is the fourth most common serotype isolated from humans, with the majority of illnesses occurring in southeastern states. the percentage of wetland cover by wetland type and the average incidence rates of serotype javiana infection in selected counties of the foodborne disease active surveillance network (foodnet) were examined. this analysis explored the relationship ... | 2017 | 28803563 |
| vibrio cholerae type 6 secretion system effector trafficking in target bacterial cells. | the type 6 secretion system (t6ss) is used by many gram-negative bacterial species to deliver toxic effector proteins into nearby bacteria prey cells to kill or inhibit their growth. vgrg proteins are core conserved secretion substrates of the t6ss and one subset of t6ss effectors consists of vgrg proteins with c-terminal extension domains carrying various enzymatic activities. in vibrio cholerae, vgrg3 has a hydrolase extension domain and degrades peptidoglycan in the periplasm of target bacter ... | 2017 | 28808000 |
| application of chitosan microparticles for mitigation of salmonella in agricultural water. | the activity of chitosan microparticles (cm) was examined using a matrix of conditions in order to assess the efficacy of cm as a mitigation against various strains of salmonella enterica in agricultural water. | 2017 | 28815882 |
| the plasmid-encoded ipf and klf fimbriae display different expression and varying roles in the virulence of salmonella enterica serovar infantis in mouse vs. avian hosts. | salmonella enterica serovar infantis is one of the prevalent salmonella serovars worldwide. different emergent clones of s. infantis were shown to acquire the pesi virulence-resistance megaplasmid affecting its ecology and pathogenicity. here, we studied two previously uncharacterized pesi-encoded chaperone-usher fimbriae, named ipf and klf. while ipf homologs are rare and were found only in s. enterica subspecies diarizonae and subspecies vii, klf is related to the known k88-fae fimbria and klf ... | 2017 | 28817673 |
| genome sequence of an australian monophasic salmonella enterica subsp. enterica typhimurium isolate (tw-stm6) carrying a large plasmid with multiple antimicrobial resistance genes. | we report the genome sequence of a monophasic salmonella enterica subsp. enterica typhimurium strain (tw-stm6) isolated in australia that is similar to epidemic multidrug-resistant strains from europe and elsewhere. this strain carries additional antibiotic and heavy-metal resistance genes on a large (275-kb) inchi2 plasmid. | 2017 | 28818900 |