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evidence for bacteria-independent hatching of trichuris muris eggs.hatching of infective larvae from embryonated eggs in the intestine is an essential first step in trichuris infections. there are three isolates of the murine parasitic nematode trichuris muris: e, e-j (the e isolate maintained in japan), and s. incubation of t. muris embryonated eggs with the intestinal bacteria escherichia coli and staphylococcus aureus induced in vitro hatching of the eggs, but enterococcus faecalis failed to induce hatching. bacteria-induced in vitro hatching of embryonated ...201323358735
development of a peptide antagonist against fsr quorum sensing of enterococcus faecalis.enterococcus faecalis fsr quorum sensing (qs) involves an 11-residue cyclic peptide named gelatinase biosynthesis-activating pheromone (gbap) that autoinduces two pathogenicity-related extracellular proteases in a cell density-dependent fashion. to identify anti-pathogenic agents that target fsr qs signaling, peptide antagonists of gbap were created by our unique drug design approach based on reverse alanine scanning. first of all, a receptor-binding scaffold (rbs), [ala(4,5,6,8,9,11)]z-gbap, wa ...201323362999
the antimicrobial activities of extract and compounds isolated from brillantaisia lamium.background:brillantaisia lamium is an erect branched herb, which grows to a height of 1.50 m in moist tropical areas, both in full sun and partial shade. in , the aerial part of this plant is used in the treatment of various microbial infections such as skin diseases and infections of urinary tract. the aim of this study was to evaluate the antimicrobial activities of ch(2)cl(2): meoh (1:1) extract, fractions and compounds from the aerial part of b. lamium.methods: the plant was dried and extrac ...201123365474
[analysis of bactericidal material generated by electrical devices advertising bactericidal ability against bacteria on the agar gel plates].several japanese companies sell electrical devices advertised as effective in inactivating viruses and killing bacteria by releasing special materials, e.g., plasmacluster ions, nanoe particle and minus ions, into the air. these companies claim that their devices killed bacteria on plates in their own experiments. we tested device effectiveness using the same experiments from the plasmacluster ioniser sharp co., japan, the nanoe generator panasonic co., japan, and the vion king jim co., japan, t ...201223367847
bacteriocin pj4 active against enteric pathogen produced by lactobacillus helveticus pj4 isolated from gut microflora of wistar rat (rattus norvegicus): partial purification and characterization of bacteriocin.the increase of multidrug-resistant pathogens and the restriction on the use antibiotics due to its side effects have drawn attention to the search for possible alternatives. bacteriocins are small antimicrobial peptides produced by numerous bacteria. much interest has been focused on bacteriocins because they exhibit inhibitory activity against pathogens. lactic acid bacteria possess the ability to synthesize antimicrobial compounds (like bacteriocin) during their growth. in this study, an anti ...201323371780
virulence traits and antibiotic resistance among enterococci isolated from eurasian otter (lutra lutra).enterococci are ubiquitous microorganisms found as part of the normal intestinal microbiota of many animals such as the free-ranging eurasian otter (lutra lutra linnaeus, 1758). in this work, twenty-nine enterococci isolated from fecal samples of eurasian otters free-living in reservoirs and associated river stretches in south portugal were identified and typed by conventional/molecular methods and screened for virulence factors and antibiotic resistance. identification allocated the isolates to ...201323375652
effects of biofilm growth on plasmid copy number and expression of antibiotic resistance genes in enterococcus faecalis.biofilm growth causes increased average plasmid copy number as well as increased copy number heterogeneity in enterococcus faecalis cells carrying plasmid pcf10. in this study, we examined whether biofilm growth affected the copy number and expression of antibiotic resistance determinants for several plasmids with diverse replication systems. four different e. faecalis plasmids, unrelated to pcf10, demonstrated increased copy number in biofilm cells. in biofilm cells, we also observed increased ...201323380728
effect of ottoman viper (montivipera xanthina (gray, 1849)) venom on various cancer cells and on microorganisms.cytotoxic and antimicrobial effects of montivipera xanthina venom against lncap, mcf-7, ht-29, saos-2, hep3b, vero cells and antimicrobial activity against selected bacterial and fungal species: staphylococcus aureus atcc 25923, escherichia coli atcc 25922, e. coli o157h7, enterococcus faecalis 29212, enterococcus faecium dsm 13590, staphylococcus epidermidis atcc 12228, s. typhimirium ccm 5445, proteus vulgaris atcc 6957 and candida albicans atcc 10239 were studied for evaluating the potential ...201423381026
detection and new genetic environment of the pleuromutilin-lincosamide-streptogramin a resistance gene lsa(e) in methicillin-resistant staphylococcus aureus of swine origin.to investigate the genetic basis of pleuromutilin resistance in porcine methicillin-resistant staphylococcus aureus (mrsa) and to map the genetic environment of the identified plasmid-borne resistance gene.201323386262
[prosthetic valve endocarditis 6 months after transcatheter aortic valve implantation].susceptibility to endocarditis after transcatheter aortic valve implantation (tavi) has not yet been described. we report the first case of acute aortic prosthetic valve endocarditis in a patient undergoing transapical tavi, successfully treated with surgery. in november 2009, an 83-year-old woman underwent tavi (23 mm edwards sapien) with an uneventful postoperative course. after 6 months, her blood cultures tested positive for enterococcus faecalis and a prosthetic aortic valve endocarditis wa ...201323389317
comparative analysis of the biological and physical properties of enterococcus faecalis bacteriophage vb_efas_gec-efs_3 and streptococcus mitis bacteriophage vb_smm_gec-smitism_2.enterococcus faecalis and streptococcus mitis are common commensal inhabitants of the human gastrointestinal and genitourinary tracts. however, both species can be opportunistic pathogens and cause disease in nosocomial settings. these infections can be difficult to treat because of the frequency of antibiotic resistance among these strains. bacteriophages are often suggested as an alternative therapeutic agent against these infections. in this study, e. faecalis and s. mitis strains were isolat ...201223391224
ampicillin plus ceftriaxone is as effective as ampicillin plus gentamicin for treating enterococcus faecalis infective endocarditis.the aim of this study was to compare the effectiveness of the ampicillin plus ceftriaxone (ac) and ampicillin plus gentamicin (ag) combinations for treating enterococcus faecalis infective endocarditis (efie).201323392394
editorial commentary: enterococcus faecalis infective endocarditis: is it time to abandon aminoglycosides? 201323392395
comparative in vitro inhibition of urinary tract pathogens by single- and multi-strain probiotics.multi-species probiotic preparations have been suggested as having a wide spectrum of application, although few studies have compared their efficacy with that of individual component strains at equal concentrations. we therefore tested the ability of 4 single probiotics and 4 probiotic mixtures to inhibit the urinary tract pathogens escherichia coli nctc 9001 and enterococcus faecalis nctc 00775.201323392622
assessment of the sealing abilities of several root canal sealers and filling methods.to evaluate the sealing abilities of two root canal sealers (epoxy resin-based ah plus(®) and polydimethylsiloxane-based guttaflow(®)) and of five root filling techniques (lateral condensation, matched taper single gutta-percha point, laterally condensed-matched taper gutta-percha point, thermafil(®) and continuous wave of condensation), using a bacterial leakage model.201323394208
effectiveness of curcumin against enterococcus faecalis biofilm.to evaluate the antimicrobial efficacy of curcumin against enterococcus faecalis biofilm formed on tooth substrate in vitro. sodium hypochlorite (naocl) and chlorhexidine (chx) served as standards for comparison.201323394209
high frequency of vancomycin-resistant enterococcus faecalis in an iranian referral children medical hospital.background: enterococci have emerged in recent years as important nosocomial pathogens. although most enterococcal human infections are caused by enterococcus faecalis, studies on vancomycin resistance are usually limited to enterococcus faecium isolates and a little is known about e. faecalis. therefore we undertook this study to obtain information about the prevalence of vancomycin -resistant e. faecalis (vref) and genes responsible for resistance.material and methods: ninety-one e. faecalis i ...201223400108
evaluation of bacterial flora during the ripening of kedong sufu, a typical chinese traditional bacteria-fermented soybean product.kedong sufu is a typical bacteria-fermented sufu in china. isolation and identification of the autochthonous bacteria involved would allow the design of specific starters for this speciality. the purpose of the present study was to evaluate the bacterial flora during the ripening of kedong sufu using polymerase chain reaction denaturing gradient gel electrophoresis (pcr-dgge) and culturing.201323400969
comparative analysis of enterococcus faecalis biofilm formation on different substrates.the aim of this study was to compare enterococcus faecalis biofilm formation on different substrates.201323402505
dentin inhibits the antibacterial effect of new and conventional endodontic irrigants.this in vitro study aimed to compare the antibacterial effect of a new irrigant, qmix, with that of conventional irrigation solutions in the presence or absence of dentin powder.201323402517
a small amount of singlet oxygen generated via excited methylene blue by photodynamic therapy induces the sterilization of enterococcus faecalis.the present study aimed to clarify the relationship between the amount of singlet oxygen ((1)o(2)) generated from excited methylene blue (mb) and the bactericidal effects on enterococcus faecalis.201323402518
towards antibacterial endodontic sealers using quaternary ammonium nanoparticles.to change and characterize the antibacterial properties of endodontic sealers by incorporating low concentrations of insoluble antibacterial nanoparticles (iabn).201323402669
formation of haloacetonitriles and haloacetamides during chlorination of pure culture bacteria.the increasing reuse of organic nitrogen-rich wastewater raises concerns regarding the formation of nitrogenous disinfection by-products (n-dbps), such as haloacetonitriles and haloacetamides. previous research mainly focused on n-dbps formation from dissolved organic matter in wastewater. in this study, dichloroacetonitrile (dcan) and dichloroacetamide (dcacam) formation from particles in the secondary effluents of a domestic wastewater treatment plant during chlorination was assessed to accoun ...201323402924
effects of antibiotics on bacterial species composition and metabolic activities in chemostats containing defined populations of human gut microorganisms.the composition and metabolic activities of the human colonic microbiota are modulated by a number of external factors, including diet and antibiotic therapy. changes in the structure and metabolism of the gut microbiota may have long-term consequences for host health. the large intestine harbors a complex microbial ecosystem comprising several hundreds of different bacterial species, which complicates investigations on intestinal physiology and ecology. to facilitate such studies, a highly simp ...201323403424
complete genome sequence of the probiotic enterococcus faecalis symbioflor 1 clone dsm 16431.here, we report the complete and annotated genome sequence of the probiotic enterococcus faecalis symbioflor 1 clone dsm 16431, included in a commercial probiotic product used for more than 50 years without any reports of infection. this sequence will provide new insights into the biology of this nonpathogenic and probiotic microorganism.201323405346
inactivation of enterococcus faecalis, pseudomonas aeruginosa and escherichia coli present in treated urban wastewater by coagulation-flocculation and photo-fenton processes.the purpose of the current study is to evaluate the inactivation of three different kinds of bacteria usually present in municipal wastewater treatment effluents (enterococcus faecalis, pseudomonas aeruginosa and escherichia coli) using a coagulation-flocculation-decantation (cfd) process combined with photo-fenton treatment at ph 5. different concentrations of fe(3+)-h2o2 (0.4/25, 5/25 and 15/25 mg l(-1)), and h2o2 (25 mg l(-1)) were evaluated for 210 minutes under artificial solar irradiation ...201323411627
comparison of antibiotic resistance, biofilm formation and conjugative transfer of staphylococcus and enterococcus isolates from international space station and antarctic research station concordia.the international space station (iss) and the antarctic research station concordia are confined and isolated habitats in extreme and hostile environments. the human and habitat microflora can alter due to the special environmental conditions resulting in microbial contamination and health risk for the crew. in this study, 29 isolates from the iss and 55 from the antarctic research station concordia belonging to the genera staphylococcus and enterococcus were investigated. resistance to one or mo ...201323411852
ochnaflavone and ochnaflavone 7-o-methyl ether two antibacterial biflavonoids from ochna pretoriensis (ochnaceae).the acetone extract of ochna pretoriensis was evaluated for antibacterial activity using bioautography and serial microplate dilution methods against four nosocomial bacterial pathogens namely: escherichia coli, staphylococcus aureus, enterococcus faecalis and pseudomonas aeruginosa. a bioassay-guided fractionation of the crude extract led to the isolation of two antibacterial biflavonoids, ochnaflavone and ochnaflavone 7-o-methyl ether. gram-negative bacteria were more sensitive to the isolated ...201223413563
high rates of multidrug resistance in enterococcus faecalis and e. faecium isolated from inpatients and outpatients in taiwan.longitudinal national data on resistance in enterococcus faecalis and e. faecium from different sources in taiwan are rare. the present study analyzed data from the taiwan surveillance of antimicrobial resistance program to address this issue. between 2002 and 2010, a total of 1696 e. faecalis and 452 e. faecium isolates were studied. although these 2 species together comprised similar percentages of all enterococci in each study year (94.1-97.2%, p = 0.19), the proportion of e. faecium increase ...201323414747
microbiological survey of imported produce available at retail across canada.increasing consumption and year-round consumer demand for fresh, minimally processed green vegetables have been observed in canada and other developed countries. however, in the past two decades, produce has been increasingly implicated in outbreaks and correspondingly recognized as a vector for the transmission of pathogenic microorganisms. to this end, we examined the microbiological quality of imported produce available at retail across canada during a period of limited domestic availability. ...201323416548
biological approach to synthesize tio2 nanoparticles using aeromonas hydrophila and its antibacterial activity.nanosized materials have been an important subject in basic and applied sciences. a novel, low-cost, green and reproducible bacteria, aeromonas hydrophila mediated biosynthesis of titanium dioxide nanoparticles (tio2 nps) was reported. the resulting nanoparticles were characterized by ftir, xrd, afm and fesem with edx. ftir showed characteristic bands (1643 and 3430 cm(-1)) finds the role of carboxyl group oh stretching amine nh stretch in the formation of tio2 nps. the xrd spectrum confirmed th ...201323416912
a rex family transcriptional repressor influences h2o2 accumulation by enterococcus faecalis.rex factors are bacterial transcription factors thought to respond to the cellular nad(+)/nadh ratio in order to modulate gene expression by differentially binding dna. to date, rex factors have been implicated in regulating genes of central metabolism, oxidative stress response, and biofilm formation. the genome of enterococcus faecalis, a low-gc gram-positive opportunistic pathogen, encodes ef2638, a putative rex factor. to study the role of e. faecalis rex, we purified ef2638 and evaluated it ...201323417491
high-level aminoglycoside resistance and virulence characteristics among enterococci isolated from recreational beaches in malaysia.we report the first study on the occurrence of high-level aminoglycoside-resistant (hlar) enterococci in coastal bathing waters and beach sand in malaysia. none of the encountered isolates were resistant to high levels of gentamicin (500 μg/ml). however, high-level resistance to kanamycin (2,000 μg/ml) was observed in 14.2 % of tested isolates, the highest proportions observed being among beach sand isolates. high-level resistance to kanamycin was higher among enterococcus faecalis and enterococ ...201323417753
chemical composition and antimicrobial and allelopathic activity of tunisian conyza sumatrensis (retz.) e.walker essential oils.conyza sumatrensis (retz.) e.walker (asteraceae) is a spontaneous annual herb, fairly widespread throughout tunisia, which has rarely been studied or valued in any sector. essential oils were obtained by hydrodistillation of different parts (flower heads, leaves, stems, and roots) of c. sumatrensis plants, which were collected in autumn (november 2007) at the flowering stage in the area of monastir, tunisia. in total, 98 compounds, representing 88.1-99.3% of the oil composition, were identified ...201323418168
dietary plant phenolic improves survival of bacterial infection in manduca sexta caterpillars.plant phenolics are generally thought to play significant roles in plant defense against herbivores and pathogens. many plant taxa, including solanaceae, are rich in phenolic compounds and some insect herbivores have been shown to acquire phenolics from their hosts to use them as protection against their natural enemies. here we demonstrate that larvae of an insect specialist on solanaceae, the tobacco hornworm, manduca sexta l. (lepidoptera: sphingidae), acquire the plant phenolic chlorogenic a ...201323420018
preparation of some eco-friendly corrosion inhibitors having antibacterial activity from sea food waste.chitosan is one of the important biopolymers and it is extracted from exoskeletons of crustaceans in sea food waste. it is a suitable eco-friendly carbon steel corrosion inhibitor in acid media; the deacetylation degree of prepared chitosan is more than 85.16 %, and the molecular weight average is 109 kda. chitosan was modified to 2-n,n-diethylbenzene ammonium chloride n-oxoethyl chitosan (compound i), and 12-ammonium chloride n-oxododecan chitosan (compound ii) as soluble water derivatives. the ...201223420292
photoelectrocatalytic disinfection of water and wastewater: performance evaluation by qpcr and culture techniques.photoelectrocatalytic oxidation (pec) was evaluated as a disinfection technique using water and secondary treated wastewater spiked with escherichia coli and enterococcus faecalis. pec experiments were carried out using a tio(2)/ti-film anode and a zirconium cathode under simulated solar radiation. bacterial inactivation was monitored by culture and quantitative polymerase chain reaction (qpcr). inactivation rates were enhanced when the duration of the treatment was prolonged and when the bacter ...201323428546
evaluation of enterococcus-infecting phages as indices of fecal pollution.no microbial source tracking tool satisfies all the characteristics of an ideal indicator of human fecal pollution. for this reason, the potential of enterococcus faecalis phages (enterophages) as markers of this type of contamination was tested by using eight enterococcus type strains as the possible hosts. the prevalence of enterophages in animal feces and domestic sewage were determined, as were the inactivation rates in raw sewage at 4 °c and surface and tap waters at 22 °c. enterophages wer ...201323428549
comparative potencies of contemporary generic vancomycin lot: in vitro assay results from nine products and a reference reagent-grade sample.numerous studies of generic vancomycin (gv) lots have emerged since the 1980s, casting some doubt on product quality. publications question the in vivo activity, even when concurrent in vitro and chemical assays meet regulatory guidelines. this study assessed contemporary gv lots by an in vitro assay capable of measuring small variations from target-benchmark (bm) activity. nine gv lots (hospira [6 lots; 0.5- or 1.0-g vials], akorn [1 lot; 1.0-g vial], app [2 lots; 1.0-g vials]) were obtained fr ...201323434498
infectious risk assessment of unsafe handling practices and management of clinical solid waste.the present study was undertaken to determine the bacterial agents present in various clinical solid wastes, general waste and clinical sharp waste. the waste was collected from different wards/units in a healthcare facility in penang island, malaysia. the presence of bacterial agents in clinical and general waste was determined using the conventional bacteria identification methods. several pathogenic bacteria including opportunistic bacterial agent such as pseudomonas aeruginosa, salmonella sp ...201323435587
biochemical and genetic characterization of the enterococcus faecalis oxaloacetate decarboxylase complex.enterococcus faecalis encodes a biotin-dependent oxaloacetate decarboxylase (oad), which is constituted by four subunits: e. faecalis carboxyltransferase subunit oada (termed ef-a), membrane pump ef-b, biotin acceptor protein ef-d, and the novel subunit ef-h. our results show that in e. faecalis, subunits ef-a, ef-d, and ef-h form a cytoplasmic soluble complex (termed ef-ahd) which is also associated with the membrane. in order to characterize the role of the novel ef-h subunit, coexpression of ...201323435880
bactericidal activity of methanol extracts of crabapple mangrove tree (sonneratia caseolaris linn.) against multi-drug resistant pathogens.the crabapple mangrove tree, sonneratia caseolaris linn. (family: sonneratiaceae), is one of the foreshore plants found in estuarine and tidal creek areas and mangrove forests. bark and fruit extracts from this plant have previously been shown to have an anti-oxidative or cytotoxic effect, whereas flower extracts of this plant exhibited an antimicrobial activity against some bacteria. according to the traditional folklore, it is medicinally used as an astringent and antiseptic. hence, this inves ...201223441048
antibiofilm activity, ph and solubility of endodontic sealers.to evaluate antibiofilm activity against enterococcus faecalis, ph and solubility of ah plus, sealer 26, epiphany se, sealapex, activ gp, mta fillapex (mta-f) and an experimental mta-based sealer (mta-s).201323441819
antimicrobial activity of pure platelet-rich plasma against microorganisms isolated from oral cavity.autologous platelet concentrates (pcs) have been extensively used in a variety of medical fields to promote soft and hard tissue regeneration. the significance behind their use lies in the abundance of growth factors in platelets α-granules that promotes wound healing. in addition, antibacterial properties of pcs against various bacteria have been recently pointed out. in this study, the antimicrobial effect of pure platelet-rich plasma (p-prp) was evaluated against oral cavity microorganisms su ...201323442413
antimicrobial activity of essential oils from mediterranean aromatic plants against several foodborne and spoilage bacteria.the antimicrobial activity of essential oils extracted from a variety of aromatic plants, often used in the portuguese gastronomy was studied in vitro by the agar diffusion method. the essential oils of thyme, oregano, rosemary, verbena, basil, peppermint, pennyroyal and mint were tested against gram-positive (listeria monocytogenes, clostridium perfringens, bacillus cereus, staphylococcus aureus, enterococcus faecium, enterococcus faecalis, and staphylococcus epidermidis) and gram-negative stra ...201323444311
[assessment of the cromocen ent chromogenic medium for clinical diagnosis of enterococcus species].the frequent incidence of enterococci at hospitals and their growing antimicrobial resistance worldwide make the in-hospital surveillance and control a pressing need; consequently, it is indispensable to avail of more sensitive and accurate diagnostic means.201223444633
engineering of a functional thermostable kanamycin resistance marker for use in moorella thermoacetica atcc39073.a transformation system for moorella thermoacetica atcc39073 was developed using thermostable kanamycin resistant gene (kanr) derived from the plasmid pjh1 that streptococcus faecalis harbored. when kanr with its native promoter was introduced into uracil auxotrophic mutant of m. thermoacetica atcc39073 together with a gene to complement the uracil auxotrophy as a selection marker, it did not give kanamycin resistance due to poor transcription level of kanr. however, the use of glyceraldehyde-3- ...201323448690
isolation and characterization of a bacteriocin-like substance produced by geobacillus toebii strain hbb-247.a total of 201 thermophilic bacteria isolated from various thermal spring, mud and soil were tested for their antibacterial activity. among the mostly active isolates, geobacillus toebii hbb-247 was further examined. bacteriocin-like inhibitory substance (blis) produced by strain hbb-247 was found to be stable up to 60°c, sensitive to proteolytic enzymes and effective against enterococcus faecalis, listeria sp., e. avium, clostridium pasteurianum, cellulomonas fimi and some thermophilic strains ...201123448995
virulence, bacterocin genes and antibacterial susceptibility in enterococcus faecalis strains isolated from water wells for human consumption.the objectives of this study were to detect genes for virulence and bacteriocins in addition to studying the antimicrobial susceptibility of 78 strains of e. faecalis isolated from water wells for human consumption. the virulence and bacteriocin genes of 78 e. faecalis were amplified by pcr and visualized in agarose gels. the antimicrobial susceptibility was determined through diffusion agar tests and the mic through microdilution. it was observed that the major percentage of virulence genes in ...201323449751
[detection of virulence genes in aminoglycoside susceptible and resistant enterococcus faecalis].enterococcus spp. is an important cause of nosocomial infections a number of virulence factors that may enhance its ability to colonize have been described. enterococcus is capable of acquiring resistance genes, including high-level resistance (hlr) to aminoglycoside antibiotics.201323450405
[microbial and pharmacological characteristics of vancogenx].prosthetic joint infection is a feared complication in total hip arthroplasty. the use of antibiotic-impregnated bone cement is the important part of preventive and therapeutic strategies. at present a number of commercial bone cements are available and support of their use by the results of experimental trials and clinical studies has varied. in relation to this issue we studied essential microbiological and pharmacological characteristics of vancogenx in comparison with gentamicin-loaded bone ...201323452425
in vitro biological effects of two anti-diabetic medicinal plants used in benin as folk medicine.extracts from polygonum senegalensis (polygonaceae) and pseudocedrela kotschyi (meliaceae) are two important traditionally used medicinal plants in rural benin to treat many diseases and notably type 2 diabetes. the aim of the study was to investigate the α-glucosidase inhibition, antioxidant and antibacterial activities of those plants extract: polygonum senegalensis leaves, and pseudocedrela kotschyi root.201323452899
topical linezolid 0.2% for the treatment of vancomycin-resistant or vancomycin-intolerant gram-positive bacterial keratitis.to report the successful use of topical linezolid 0.2% in the treatment of gram-positive bacterial keratitis.201323453280
effect of high-intensity focused ultrasound on enterococcus faecalis planktonic suspensions and biofilms.in this study, the effect of high-intensity focused ultrasound (hifu) on enterococcus faecalis on both planktonic suspensions and biofilms was investigated. e. faecalis persist in secondary dental infections as biofilms. glass-bottom petri dishes with biofilms were centered at the focal point of the hifu wave generated by a 250-khz transducer. specimens were subjected to hifu exposure at different periods of 30, 60 and 120 s. the viable bacteria, removal effect and bacterial viability of biofilm ...201323453374
occurrence, genetic diversity, and persistence of enterococci in a lake superior watershed.in 2012, the u.s. epa suggested that coastal and great lakes states adopt enterococci as an alternative indicator for the monitoring of recreational water quality. limited information, however, is available about the presence and persistence of enterococci in lake superior. in this study, the density, species composition, and persistence of enterococci in sand, sediment, water, and soil samples were examined at two sites in a lake superior watershed from may to september over a 2-year period. th ...201323455345
diversity of streptococcus mutans strains in bacterial interspecies interactions.biofilms are matrix-enclosed microbial population adhere to each other and to surfaces. compared to planktonic bacterial cells, biofilm cells show much higher levels of antimicrobial resistance. we aimed to investigate streptococcus mutans strain diversity in biofilm formation and chlorhexidine (chx) resistance of single s. mutans and dual s. mutans-enterococcus faecalis biofilms. four clinical s. mutans strains (c180-2, c67-1, hg723 and ua159) formed 24-h biofilms with or without an e. faecalis ...201423456658
ahrc and eep are biofilm infection-associated virulence factors in enterococcus faecalis.enterococcus faecalis is part of the human intestinal microbiome and is a prominent cause of health care-associated infections. the pathogenesis of many e. faecalis infections, including endocarditis and catheter-associated urinary tract infection (cauti), is related to the ability of clinical isolates to form biofilms. to identify chromosomal genetic determinants responsible for e. faecalis biofilm-mediated infection, we used a rabbit model of endocarditis to test strains with transposon insert ...201323460519
undecaprenyl pyrophosphate phosphatase confers low-level resistance to bacitracin in enterococcus faecalis.undecaprenyl pyrophosphate phosphatases (uppps) have been implicated in bacitracin resistance in some bacterial genera and the aim of this study was to determine the role of uppps in mediating low-level bacitracin resistance in enterococcus faecalis.201323460607
east north central region has the highest prevalence of vancomycin-resistant enterococcus faecalis in the united states. 201323466924
draft genome sequence of enterococcus faecalis pc1.1, a candidate probiotic strain isolated from human feces.enterococcus faecalis is commonly isolated from the gastrointestinal tract of healthy infants and adults, where it contributes to host health and well-being. we describe here the draft genome sequence of e. faecalis pc1.1, a candidate probiotic strain isolated from human feces.201323469340
vancomycin resistant enterococci: from the hospital effluent to the urban wastewater treatment plant.vancomycin is an important antibiotic to treat serious nosocomial enterococci infections. human activities, in particular those related with clinical practices performed in hospitals, can potentiate the transfer and selection of clinically-relevant resistant bacteria such as vancomycin resistant enterococci (vre). indeed, previous studies demonstrated the occurrence of vre in urban wastewater treatment plants and related environments (e.g. sewage, rivers). in this study, the occurrence of vre in ...201323474261
new water-soluble polypyridine silver(i) derivatives of 1,3,5-triaza-7-phosphaadamantane (pta) with significant antimicrobial and antiproliferative activities.the new series of silver(i) coordination polymers [ag(n-n)(μ-pta)]n(x)n (1, 2, 4-8, 10, 11) and discrete monomers [ag(n-n)(pta)2](x) (3, 9) {n-n = bpy (1-3), dtbpy (4), neocup (5, 6), phen (7-9), dione (10, 11); x = no3 (1, 3, 5, 7, 9, 10), pf6 (2, 4, 6, 8, 11)} were generated by self-assembly reactions, in meoh at ~25 °c, of agno3 or agpf6 with 1,3,5-triaza-7-phosphaadamantane (pta) and the corresponding polypyridines, namely 2,2'-bipyridine (bpy), 4,4'-di-tert-butyl-2,2'-bipyridine (dtbpy), 1, ...201323474654
predominant role of host proteases in myocardial damage associated with infectious endocarditis induced by enterococcus faecalis in a rat model.infective endocarditis (ie) remains a life-threatening infectious disease with high morbidity and mortality. the objectives of the present study are to assess the host proteolytic activities of the vegetations and their cytotoxic potential in a rat model of experimental ie. rats were infected with a strain of enterococcus faecalis of particularly low virulence and weak protease expression. we tested the presence of proteases released by infiltrated leukocytes (matrix metalloproteinases and elast ...201323478315
substituted 1,6-diphenylnaphthalenes as ftsz-targeting antibacterial agents.bacterial cell division occurs in conjunction with the formation of a cytokinetic z-ring structure comprised of ftsz subunits. agents that disrupt z-ring formation have the potential, through this unique mechanism, to be effective against several of the newly emerging multidrug-resistant strains of infectious bacteria. several 1-phenylbenzo[c]phenanthridines exhibit notable antibacterial activity. based upon their structural similarity to these compounds, a distinct series of substituted 1,6-dip ...201323481648
isolated pulmonary valve endocarditis in a patient with aplastic anaemia.a 42-year-old female patient of aplastic anaemia on maintenance blood transfusion presented with a 3-week history of fever, cough, dyspnoea and pedal oedema. upon examination she  was found to have severe pallor, temperature of 101°f, tachycardia, bilateral pitting pedal oedema, raised jugular venous pressure, ejection systolic murmur (grade 2/6) in pulmonary area and petechiae over extensor aspect of both lower limbs. blood investigations revealed low haemoglobin, thrombocytopaenia and mild inc ...201323483061
antibacterial effects of two different types of laser and aqueous ozone against enterococcus faecalis in root canals.the purpose of this study was to investigate the antibacterial effects of two different types of laser and aqueous ozone in human root canals infected by enterococcus faecalis.201323484588
antibiotics and heavy metals resistance and other biological characters in enterococci isolated from surface water of monte cotugno lake (italy).considering the limited knowledge about the biological characters in enterococci isolated from surface waters, we investigated antibiotic and heavy-metal resistance, bacteriocin production, and some important virulence traits of 165 enterococci collected in water samples from monte cotugno lake, the largest artificial basin built with earth in europe. the species distribution of isolates was as follows: enterococcus faecium (80%), enterococcus faecalis (12.7%), enterococcus casseliflavus (3%), e ...201323485245
ex vivo antimicrobial efficacy of strong acid electrolytic water against enterococcus faecalis biofilm.to observe the antimicrobial effect of strong acid electrolytic water (saew) against an enterococcus faecalis (e. faecalis) biofilm when used as a root canal irrigant.201323488685
antimicrobial activity and hydrophobicity of edible whey protein isolate films formulated with nisin and/or glucose oxidase.the use of edible antimicrobial films has been reported as a means to improve food shelf life through gradual releasing of antimicrobial compounds on the food surface. this work reports the study on the incorporation of 2 antimicrobial agents, nisin (n), and/or glucose oxidase (go), into the matrix of whey protein isolate (wpi) films at ph 5.5 and 8.5. the antimicrobial activity of the edible films was evaluated against listeria innocua (atcc 33090), brochothrix thermosphacta (ncib10018), escher ...201323488765
enterococcus faecalis utilizes maltose by connecting two incompatible metabolic routes via a novel maltose 6'-phosphate phosphatase (mapp).similar to bacillus subtilis, enterococcus faecalis transports and phosphorylates maltose via a phosphoenolpyruvate (pep):maltose phosphotransferase system (pts). the maltose-specific pts permease is encoded by the malt gene. however, e. faecalis lacks a mala gene encoding a 6-phospho-α-glucosidase, which in b. subtilis hydrolyses maltose 6'-p into glucose and glucose 6-p. instead, an operon encoding a maltose phosphorylase (malp), a phosphoglucomutase and a mutarotase starts upstream from malt. ...201323490043
antimicrobial effect of alexidine and chlorhexidine against enterococcus faecalis infection.a previous study demonstrated that alexidine has greater affinity for the major virulence factors of bacteria than chlorhexidine. the aim of this study was to compare the antimicrobial activity of 1% alexidine with that of 2% chlorhexidine using enterococcus faecalis-infected dentin blocks. sixty bovine dentin blocks were prepared and randomly divided into six groups of 10 each. e. faecalis was inoculated on 60 dentin blocks using the luppens apparatus for 24 h and then the dentin blocks were so ...201323492900
evaluation of the antibacterial activity of three gutta-percha solvents against enterococcus faecalis.enterococcus faecalis (e. faecalis) is implicated as the common etiological agent of root canal treatment failure. the study was aimed to compare the antibacterial efficacy and to assess the minimum inhibitory concentration (mic) of 3 gutta-percha solvents-rc solve, endosolv-e, and xylene against e. faecalis.201323504811
bacteria isolated from dugongs (dugong dugon) submitted for postmortem examination in queensland, australia, 2000-2011.microbial infection may contribute to disease in a significant proportion of marine mammal mortalities, but little is known about infectious bacterial species and their prevalence in dugongs (dugong dugon). this study represents a survey of the species of bacteria and fungi isolated from dugongs submitted to the university of queensland's school of veterinary science for postmortem examination. thirty-six dugongs were included in the survey, with 23 species of bacteria and four species of fungus ...201323505701
comparative evaluation of antimicrobial efficacy of syzygium aromaticum, ocimum sanctum and cinnamomum zeylanicum plant extracts against enterococcus faecalis: a preliminary study.to evaluate the antimicrobial efficacy of ocimum sanctum, cinnamomum zeylanicum, syzygium aromaticum and 3% sodium hypochlorite (naocl) against enterococcus faecalis in planktonic suspension and biofilm phenotypes.201323506110
a liaf codon deletion abolishes daptomycin bactericidal activity against vancomycin-resistant enterococcus faecalis.the genetic bases for antibiotic tolerance are obscure. daptomycin (dap) is a lipopeptide antibiotic with bactericidal activity against enterococci. using time-kill assays, we provide evidence for the first time that a deletion of isoleucine in position 177 of liaf, a member of the three-component regulatory system liafsr involved in the cell envelope response to antimicrobials, is directly responsible for a dap-tolerant phenotype and is likely to negatively affect response to dap therapy.201323507277
effect of initial irrigation with sodium hypochlorite on residual antibacterial activity of tetraclean.the purpose of this study was to assess the effect of dentin pretreatment with sodium hypochlorite (naocl) on the antibacterial substantivity of tetraclean in bovine root dentin in vitro. one hundred and forty dentin tubes prepared from bovine incisor teeth were infected with enterococcus faecalis for 14 days. the specimens were divided into five groups as follows: tetraclean; 5.25% naocl/tetraclean; 2% chlorhexidine (chx); infected dentin tubes (positive control); and sterile dentin tubes (nega ...201323513547
antimicrobial activity of daptomycin tested against gram-positive pathogens collected in europe, latin america, and selected countries in the asia-pacific region (2011).we report the results of the international daptomycin surveillance programs for europe, latin america, and selected asia-pacific nations. a total of 7948 consecutive gram-positive organisms of clinical significance were collected in 2011 and susceptibility tested against daptomycin and various comparator agents by clinical and laboratory standards institute (clinical and laboratory standards institute. m07-a9. methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobic ...201323514757
antibacterial effect of different root canal sealers on three bacterial species.bacteria persisting in the root canal system may thwart endodontic therapy. it is therefore interesting to know whether clinically available root canal sealers have an antimicrobial effect. the objective of the present in vitro study was to investigate the antibacterial effect of various sealers on the endodontologically detectable species enterococcus faecalis (e. faecalis), fusobacterium nucleatum (f. nucleatum) and porphyromonas gingivalis (p. gingivalis).201323523285
effectiveness of a high purity chlorine dioxide solution in eliminating intracanal enterococcus faecalis biofilm.we investigated the effectiveness of chlorine dioxide (clo2) solution in comparison to sodium hypochlorite (naocl) and chlorhexidine gluconate (chx) in the elimination of intracanal enterococcus faecalis biofilm. extracted human teeth were inoculated with e. faecalis. after preparation the canals were irrigated with clo2, naocl, chx or physiologic saline for control. two and five days later bacterial samples were collected and streaked onto columbia agar. cfu/ml were counted. the canal walls wer ...201323529300
crisprs of enterococcus faecalis and e. hirae isolates from pig feces have species-specific repeats but share some common spacer sequences.clustered regularly interspaced short palindromic repeats (crispr) are currently a topic of interest in microbiology due to their role as a prokaryotic immune system. investigations of crispr distribution and characterization to date have focused on pathogenic bacteria, while less is known about crispr in commensal bacteria, where they may have a significant role in the ecology of the microbiota of humans and other animals, and act as a recorder of interactions between bacteria and viruses. a co ...201323535981
nasal and perirectal colonization of vancomycin sensitive and resistant enterococci in patients of paediatrics icu (picu) of tertiary health care facilities.enterococci normally inhabit the intestinal tract of humans and are also a potential pathogen in causing nosocomial infections. the increase in antibiotic resistance and transfer of antibiotic resistance gene to staphylococcus aureus (s. aureus) due to co-colonization has increased its importance in research. the aim of the study was to evaluate local epidemiology of nasal and rectal colonization with enterococcus faecalis (e. faecalis) and enterococcus faecium (e. faecium) in patients of paedia ...201323536967
in vitro assessment of functional properties of lactic acid bacteria isolated from faecal microbiota of healthy dogs for potential use as probiotics.lactic acid bacteria were isolated and identified in the faeces of chinese crested and yorkshire terrier pups and their probiotic features were investigated in vitro. thirty seven isolates were identified as lactobacillus or enterococcus. out of these isolates, 31 were lactic acid bacteria (lab) and belonged to the species lactobacillus reuteri (16/37; 43.3%), lactobacillus animalis (7/37; 18.9%), lactobacillus acidophilus (3/37; 8.1%), lactobacillus sanfranciscensis (2/37; 5.4%), lactobacillus ...201323538205
new trends in dentistry: plant extracts against enterococcus faecalis. the efficacy compared to chlorhexidine.enterococcus faecalis is an important pathogen associated with endodontic diseases, and its elimination and control are of paramount importance, as it represents one of the major causes of failure in the treatment of endodontic disease. twenty-five plant extracts obtained from brazilian forests were found to be effective against planktonic e. faecalis and were subjected to two traditional antibacterial assays, the microdilution broth assay (mdba) and the disk diffusion assay (dda), using chlorhe ...201323538423
enterococcus faecalis infective endocarditis: a pilot study of the relationship between duration of gentamicin treatment and outcome.because of the nephrotoxic effects of aminoglycosides, the danish guidelines on infective endocarditis were changed in january 2007, reducing gentamicin treatment in enterococcal infective endocarditis from 4 to 6 weeks to only 2 weeks. in this pilot study, we compare outcomes in patients with enterococcus faecalis infective endocarditis treated in the years before and after endorsement of these new recommendations.201323543002
a new era for treating enterococcus faecalis endocarditis: ampicillin plus short-course gentamicin or ampicillin plus ceftriaxone: that is the question! 201323543003
cd137 expressed on neutrophils plays dual roles in antibacterial responses against gram-positive and gram-negative bacterial infections.severe sepsis and septic shock caused mainly by bacterial infections are life-threatening conditions that urge the development of novel therapies. however, host responses to and pathophysiology of sepsis have not been clearly understood, which remains a major obstacle for the development of effective therapeutics. recently, we have shown that stimulation of a costimulatory molecule, cd137, enhanced survival of mice infected with the gram-positive (g(+)) intracellular bacterium listeria monocytog ...201323545301
expression of the collagen adhesin ace by enterococcus faecalis strain og1rf is not repressed by ers but requires the ers box.expression of adhesin to collagen of enterococcus faecalis (ace), a known virulence factor, is increased by environmental signals such as the presence of serum, high temperature, and bile salts. currently, the enterococcal regulator of survival (ers) of e. faecalis strain jh2-2 is the only reported repressor of ace. here, we show that for strain og1rf, ers is not involved in the regulation of ace. our data showed similar levels of ace expression by og1rf and its δers derivative in the presence o ...201323551253
evaluation of the antimicrobial activities of chlorhexidine gluconate, sodium hypochlorite and octenidine hydrochloride in vitro.the objective of this study was to compare the antimicrobial activity of sodium hypochlorite (naocl), chlorhexidine gluconate (chx) and octenidine hydrochloride (oct) in different concentrations against endodontic pathogens in vitro. agar diffusion procedure was used to determine the antimicrobial activity of the tested materials. enterococcus faecalis, candida albicans and the mixture of these were used for this study. in the agar diffusion test, 5.25% naocl exhibited better antimicrobial effec ...201323551508
inactivation of staphylococcus aureus and enterococcus faecalis by a direct-current, cold atmospheric-pressure air plasma microjet.a direct-current, cold atmospheric-pressure air plasma microjet (pmj) was performed to inactivate staphylococcus aureus (s. aureus) and enterococcus faecalis (e. faecalis) in air. the process of sterilization and morphology of bacteria was observed. we wish to know the possible inactivation mechanisms of pmj and explore a potential application in dental and other temperature sensitive treatment.201023554639
antibacterial activity of calcium hydroxide combined with chitosan solutions and the outcomes on the bond strength of realseal sealer to radicular dentin.the purpose of this study was to investigate the antibacterial activity of calcium hydroxide [ca(oh)2] combined with chitosan solutions against enterococcus faecalis-infected root canal dentin and the effect of this new intracanal medicament on the bond strength of realseal sealer to radicular dentin. an experimental intracanal medicament was prepared by mixing different concentrations of chitosan solution (25%, 50%, and 100%, w/v) to ca(oh)2 powder. antibacterial activity was evaluated and the ...201223554749
effects of lysed enterococcus faecalis fk-23 on experimental allergic rhinitis in a murine model.in the current study, we sought to investigate whether lysed enterococcus faecalis fk-23 (lfk), a heat-killed probiotic preparation, attenuated eosinophil influx into the upper airway and had immunomodulatory activity in a murine allergic rhinitis model. eighteen balb/c mice were divided into three groups; the ovalbumin (ova)-sensitized/challenged group, which received saline orally for 6 weeks (ova group), the ova-sensitized/challenged group, which received lfk orally for 6 weeks (lfk-fed group ...201223554753
effect of addition of chitosan to self-etching primer: antibacterial activity and push-out bond strength to radicular dentin.the purpose of this study was to evaluate the antibacterial activity of a modified self-etching primer incorporating chitosan and whether this modification affected the bond strength to radicular dentin. a modified self-etching primer was prepared by adding chitosan solutions at 0.03%, 0.06%, 0.12% and 0.25% (w/w) to realseal selfe-tching primer. realseal primer without chitosan was used as the control. the antibacterial activity of the modified self-etching primer was evaluated using the direct ...201223554762
microevolutionary events involving narrow host plasmids influences local fixation of vancomycin-resistance in enterococcus populations.vancomycin-resistance in enterococci (vre) is associated with isolates within st18, st17, st78 enterococcus faecium (efm) and st6 enterococcus faecalis (efs) human adapted lineages. despite of its global spread, vancomycin resistance rates in enterococcal populations greatly vary temporally and geographically. portugal is one of the european countries where tn1546 (vana) is consistently found in a variety of environments. a comprehensive multi-hierarchical analysis of vre isolates (75 efm and 29 ...201323555993
identification of vancomycin-resistant enterococci clones and inter-hospital spread during an outbreak in taiwan.in 2003, nosocomial infections caused by vancomycin-resistant enterococci (vre) occurred rarely in taiwan. between 2003 and 2010, however, the average prevalence of vancomycin resistance among enterococci spp. increased from 2% to 16% in community hospitals and from 3% to 21% in medical centers of taiwan. we used molecular methods to investigate the epidemiology of vre in a tertiary teaching hospital in taiwan.201323556473
antimicrobial resistance of enterococcus species from meat and fermented meat products isolated by a pcr-based rapid screening method.enterococci are predominantly found in the gastrointestinal tract of humans and animals, but species commonly resident on vegetation are known. their presence in large numbers in foods may indicate a lapse in sanitation and their ability to serve as a genetic reservoir of transferable antibiotic resistance is of concern. conventional culture methods for identification of enterococci are slow and sometimes give false results because of the biochemical diversity of the organisms in this genus. thi ...201323558191
effect of zingiber officinale and propolis on microorganisms and endotoxins in root canals.the purpose of this study was to evaluate the effectiveness of glycolic propolis (pro) and ginger (gin) extracts, calcium hydroxide (ch), chlorhexidine (clx) gel and their combinations as icms (icms) against candida albicans, enterococcus faecalis, escherichia coli and endotoxins in root canals.201723559108
ex vivo microbial leakage after using different final irrigation regimens with chlorhexidine.to assess the influence of final irrigation protocols with chlorhexidine in the coronal leakage of enterococcus faecalis in filled root canals.201723559116
antimicrobial effects of calcium hydroxide, chlorhexidine, and propolis on enterococcus faecalis and candida albicans.to evaluate the efficacy of calcium hydroxide (ca[oh]2 ), 2% chlorhexidine (chx) gel, and propolis against both enterococcus faecalis (e. faecalis) and candida albicans (c. albicans) using infected dentine models at two different depths (100 and 200 μm) after 14 days of application.201423559565
comparison of the bactericidal efficacy of photodynamic therapy, 2.5% sodium hypochlorite, and 2% chlorhexidine against enterococcous faecalis in root canals; an in vitro study.enterococcus faecalis has been widely used as a valuable microbiological pathogen for in vitro studies due to its ability to successfully colonize the root canal in a biofilm-like style, invade dentinal tubules, and resist endodontic treatment procedures.the aim of this study was to compare the bactericidal efficacy of photodynamic therapy (pdt), 2% chlorhexidine, 2.5% naocl, and combination of pdt and 2.5% naocl against e. faecalis.201223559928
comparison of enterococcus faecium and enterococcus faecalis strains isolated from water and clinical samples: antimicrobial susceptibility and genetic relationships.enterococci are part of the normal intestinal flora in a large number of mammals, and these microbes are currently used as indicators of fecal contamination in water and food for human consumption. these organisms are considered one of the primary causes of nosocomial and environmental infections due to their ability to survive in the environment and to their intrinsic resistance to antimicrobials. the aims of this study were to determine the biochemical patterns and antimicrobial susceptibiliti ...201323560050
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