Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| attribution of human listeria monocytogenes infections in england and wales to ready-to-eat food sources placed on the market: adaptation of the hald salmonella source attribution model. | human listeriosis is a rare but serious foodborne disease, with high morbidity and mortality in vulnerable populations (e.g., pregnant women, the elderly, and the immunocompromised). the disease is predominantly caused by the consumption of contaminated ready-to-eat foods. since 2001, an increase in the number of listeriosis cases has been observed in several european union countries, including england and wales, predominantly in the over-60s population. the cause of this selective increased inc ... | 2010 | 20156087 |
| listeria monocytogenes as a vector for anti-cancer therapies. | the intracellular pathogen listeria monocytogenes represents a promising therapeutic vector for the delivery of dna, rna or protein to cancer cells or to prime immune responses against tumour-specific antigens. a number of biological properties make l. monocytogenes a promising platform for development as a vector for either gene therapy or as an anti-cancer vaccine vector. l. monocytogenes is particularly efficient in mediating internalization into host cells. once inside cells, the bacterium p ... | 2010 | 20158470 |
| pregnancy-associated listeriosis. | infection by listeria monocytogenes in pregnant women may result in fetal loss or invasive disease in the newborn. we examined listeriosis cases reported through the u.s. listeria initiative during 2004-2007. cases were classified as pregnancy-associated if illness occurred in a pregnant woman or an infant aged <28 days. of 758 reported listeria cases, 128 (16.9%) were pregnancy-associated. maternal infection resulted in four neonatal deaths and 26 (20.3%) fetal losses. invasive illnesses in new ... | 2010 | 20158931 |
| microbiological and molecular investigation of an increase of human listeriosis in belgium, 2006-2007. | in belgium, the majority of cases of listeriosis are sporadic cases. in this study we present evidence for an episode of listeriosis: a time-linked cluster of cases that occurred in 2006 and 2007, and the identification of identical strains. the episode involved 11 patients, infected with listeria monocytogenes of serovar 4b. the source of infection was not detected. | 2010 | 20158978 |
| influenza virus-induced glucocorticoids compromise innate host defense against a secondary bacterial infection. | multicellular organisms are continuously exposed to many different pathogens. because different classes of pathogens require different types of immune responses, understanding how an ongoing immune response to one type of infection affects the host's ability to respond to another pathogen is essential for a complete understanding of host-pathogen interactions. here, we used a mouse model of coinfection to gain insight into the effect of respiratory influenza virus infection on a subsequent syste ... | 2010 | 20159617 |
| community-acquired bacterial meningitis in alcoholic patients. | alcoholism is associated with susceptibility to infectious disease, particularly bacterial pneumonia. in the present study we described characteristics in alcoholic patients with bacterial meningitis and delineate the differences with findings in non-alcoholic adults with bacterial meningitis. | 2010 | 20161709 |
| antimicrobial activity of various extracts of ocimum basilicum l. and observation of the inhibition effect on bacterial cells by use of scanning electron microscopy. | the antimicrobial activities of chloroform, acetone and two different concentrations of methanol extracts of ocimum basilicum l. were studied. these extracts were tested in vitro against 10 bacteria and 4 yeasts strains by the disc diffusion method. the results indicated that the methanol extracts of o. basilucum exhibited the antimicrobial activity against tested microorganisms. while the chloroform and acetone extracts had no effect, the methanol extracts showed inhibition zones against strain ... | 2008 | 20161958 |
| alpha-galcer ameliorates listeriosis by accelerating infiltration of gr-1+ cells into the liver. | alpha-galactosylceramide (alpha-galcer) activates invariant (i)nkt cells, which in turn stimulate immunocompetent cells. although activation of inkt cells appears critical for regulation of immune responses, it remains elusive whether protection against intracellular bacteria can be induced by alpha-galcer. here, we show that alpha-galcer treatment ameliorates murine listeriosis, and inhibits inflammation following listeria monocytogenes infection. liver infiltration of gr-1+ cells and gamma/del ... | 2010 | 20162550 |
| a regulatory role for macrophage class a scavenger receptors in tlr4-mediated lps responses. | recognition of microbial components by tlr, key sensors of infection, leads to induction of inflammatory responses. we found that, in vivo, tlr4 engagement by lps induces up-regulation of the class a scavenger receptors (sr) macrophage receptor with a collagenous structure (marco) and sr-a, which occurs, at least in the case of marco, via both myd88-dependent and -independent pathways. when challenging mice with a low dose of lps followed by a high dose, class a sr-deficient mice showed a higher ... | 2010 | 20162551 |
| h2-m3-restricted cd8+ t cells augment cd4+ t-cell responses by promoting dc maturation. | infection with listeria monocytogenes triggers the activation and expansion of nonconventional cd8+ t cells restricted by the mhc class ib molecule, h2-m3. h2-m3-restricted cd8+ t cells exhibit a memory phenotype, rapidly produce cytokines, and reach peak frequencies sooner than conventional mhc class ia-restricted cd8+ t cells. in this study, we found that simultaneous in vivo priming of h2-m3-restricted t cells and adoptively transferred ot-ii cd4+ t cells on the same dc enhances the survival ... | 2010 | 20162552 |
| turning riboswitches loose. | 2010 | 20162658 | |
| characterization of bacteriocin produced by streptococcus bovis j2 40-2 isolated from traditional fermented milk 'dahi'. | a bacteriocin-producing strain streptococcus bovis j2 40-2 was isolated from traditional fermented milk 'dahi' in bangladesh. despite its narrow antimicrobial spectrum, it showed strong antimicrobial activity against extremely challenging and problematic organisms in foods, such as listeria monocytogenes. bacteriocin was sensitive to several proteolytic enzymes and showed antimicrobial activity over a wide ph range of 2.0-10.0. it was stable when heated to 110 degrees c for 20 min, but lost 25% ... | 2009 | 20163471 |
| diversity of the cd8+ t cell repertoire elicited against an immunodominant epitope does not depend on the context of infection. | the diversity of the pathogen-specific t cell repertoire is believed to be important in allowing recognition of different pathogen epitopes and their variants and thereby reducing the opportunities for mutation-driven pathogen escape. however, the extent to which the tcr repertoire can be manipulated by different vaccine strategies so as to obtain broad diversity and optimal protection is incompletely understood. we have investigated the influence of the infectious/inflammatory context on the tc ... | 2010 | 20164421 |
| estimation of the pod function and the lod of a qualitative microbiological measurement method. | qualitative microbiological measurement methods in which the measurement results are either 0 (microorganism not detected) or 1 (microorganism detected) are discussed. the performance of such a measurement method is described by its probability of detection as a function of the contamination (cfu/g or cfu/ml) of the test material, or by the lod(p), i.e., the contamination that is detected (measurement result 1) with a specified probability p. a complementary log-log model was used to statistical ... | 2009 | 20166595 |
| high-throughput genome sequencing of two listeria monocytogenes clinical isolates during a large foodborne outbreak. | a large, multi-province outbreak of listeriosis associated with ready-to-eat meat products contaminated with listeria monocytogenes serotype 1/2a occurred in canada in 2008. subtyping of outbreak-associated isolates using pulsed-field gel electrophoresis (pfge) revealed two similar but distinct asci pfge patterns. high-throughput pyrosequencing of two l. monocytogenes isolates was used to rapidly provide the genome sequence of the primary outbreak strain and to investigate the extent of genetic ... | 2010 | 20167121 |
| the impact of environmental stress on listeria monocytogenes virulence. | listeria monocytogenes, a significant food-borne pathogen, must defy a variety of conditions encountered in the food environment and during the infection process. in reaction to adverse conditions, the bacteria significantly change their metabolism, inducing a stress response which is mediated by a range of alternative sigma factors. the extent of the response to stress was shown to vary in the l. monocytogenes population. according to recent evidence a major l. monocytogenes alternative sigma f ... | 2009 | 20169937 |
| synthetic human chorionic gonadotropin-related oligopeptides impair early innate immune responses to listeria monocytogenes in mice. | background. synthetic human chorionic gonadotropin (hcg)-related oligopeptides are potent inhibitors of pathogenic inflammatory responses induced by in vivo lipopolysaccharide exposure or hemorrhagic shock-induced injury. in this study, we tested whether hcg-related oligopeptide treatment similarly altered inflammatory responses and innate host defenses in mice during experimental listeria monocytogenes infection. methods. mice were infected with l. monocytogenes and treated with hcg-related oli ... | 2010 | 20170375 |
| evaluation of diffusion and dilution methods to determine the antibacterial activity of plant extracts. | the aim of this study was to evaluate diffusion and dilution methods for determining the antibacterial activity of plant extracts and their mixtures. several methods for measurement of the minimal inhibitory concentration (mic) of a plant extract are available, but there is no standard procedure as there is for antibiotics. we tested different plant extracts, their mixtures and phenolic acids on selected gram-positive (staphylococcus aureus, bacillus cereus, and listeria monocytogenes) and gram- ... | 2010 | 20171250 |
| autonomous growth of isolated single listeria monocytogenes and salmonella enterica serovar typhimurium cells in the absence of growth factors and intercellular contact. | the aim of this study was to observe growth of isolated single bacterial cells in the absence of growth factors and intercellular contact. in order to exclude stochastic uncertainties induced by dilution series, a new micromanipulation method was developed to ensure explicit results under visual control. this was performed with particular care for production of single prokaryotic cells and subsequent investigation of their autonomous growth. over 450 single isolated listeria monocytogenes and sa ... | 2010 | 20173058 |
| phenolics and anthocyanins in berries of european cranberry and their antimicrobial activity. | european cranberry is a berry plant rich in biologically active substances, making it valued by both the phyto-pharmaceutical and food industries. the aim of this study was to examine the accumulation of phenolic compounds and anthocyanins in berries of european cranberry and to assess their antibacterial activity. material and methods: different wild clones of european cranberry were investigated according to berry weight and the amounts of total phenolics and anthocyanins. anthocyanin profiles ... | 2009 | 20173403 |
| listeria endocarditis in a patient with psoriatic arthritis on infliximab: are biologic agents as treatment for inflammatory arthritis increasing the incidence of listeria infections? | the use of anti-tumor necrosis factor agents such as infliximab as treatment modalities of inflammatory joint diseases has widely spread over the past few years. however, increasing numbers of reports of infectious complications during tnf-a blockade have also highlighted the fact that an increased rate of sometimes life-threatening complications may be the price paid for superior therapeutic efficacy. we report the first case report of listeria endocarditis associated with infliximab use and th ... | 2010 | 20176052 |
| type i ifn signaling constrains il-17a/f secretion by gammadelta t cells during bacterial infections. | recognition of intracellular bacteria by macrophages leads to secretion of type i ifns. however, the role of type i ifn during bacterial infection is still poorly understood. francisella tularensis, the causative agent of tularemia, is a pathogenic bacterium that replicates in the cytosol of macrophages leading to secretion of type i ifn. in this study, we investigated the role of type i ifns in a mouse model of tularemia. mice deficient for type i ifn receptor (ifnar1(-/-)) are more resistant t ... | 2010 | 20176744 |
| the stress-induced virulence protein inlh controls interleukin-6 production during murine listeriosis. | the genome of the pathogenic bacterium listeria monocytogenes contains a family of genes encoding proteins with a leucine-rich repeat domain. one of these genes, inlh, is a sigma(b)-dependent virulence gene of unknown function. previously, inlh was proposed to be coexpressed with two adjacent internalin genes, inlg and inle. using tiling arrays, we showed that inlh expression is monocistronic and specifically induced in stationary phase as well as in the intestinal lumen of mice, independent of ... | 2010 | 20176794 |
| survey of listeria monocytogenes and other listeria sp. contamination in different common ready-to-eat food products in jordan. | incidence and contamination levels of different listeria monocytogenes serovars and different listeria sp. in 360 samples of common ready-to-eat food products in jordan were investigated. the presence of l. monocytogenes was determined using en iso protocol and confirmed using pcr technique. five listeria sp.: l. monocytogenes, l. innocua, l. ivanovvi, l. welshimeri and l. seeligeri were isolated. l. monocytogenes was isolated from 19 samples (5.3%), from 6 rte dairy samples; 6 rte vegetables sa ... | 2009 | 20180325 |
| reduced host cell invasiveness and oxidative stress tolerance in double and triple csp gene family deletion mutants of listeria monocytogenes. | the cold shock protein (csp) family comprises small, highly conserved proteins that bind nucleic acids to modulate various bacterial gene expressions. in addition to cold adaptation functions, this group of proteins is thought to facilitate various cellular processes to promote normal growth and stress adaptation responses. three proteins making up the listeria monocytogenes csp family (cspa, cspb, and cspd) promote both cold and osmotic stress adaptation functions in this bacterium. the contrib ... | 2010 | 20184451 |
| the bacteriological quality of retail-level peanut, almond, cashew, hazelnut, brazil, and mixed nut kernels produced in two australian nut-processing facilities over a period of 3 years. | an investigation of the bacteriological quality of ready-to-eat peanuts, almonds, cashews, hazelnuts, and brazil nuts processed in two australian nut-processing facilities was performed over a period of 3 years. a total of 564 ready-to-eat retail packs were analyzed for aerobic plate count (apc), salmonella, coagulase-positive staphylococci, listeria monocytogenes, and escherichia coli. of these samples, 343 were peanuts, 45 cashews, 42 almonds, 40 brazil nuts, 51 hazelnuts, and 43 were mixed pa ... | 2010 | 20184453 |
| outbreak of listeriosis caused by infected beef meat from a meals-on-wheels delivery in denmark 2009. | an outbreak of listeriosis in denmark occurred in may 2009. multilocus variable number of tandem repeats analysis typing, later confirmed by pulsed-field gel electrophoresis typing, showed that isolates from eight patients had identical patterns and were distinguishable from listeria monocytogenes isolates from other danish patients. seven out of eight patients had received a meal with beef from the same meals-on-wheels delivery catering company 3 weeks prior to onset of disease. two patients di ... | 2011 | 20184622 |
| light contributes to early but not late control of mycobacterium tuberculosis infection. | the tnf superfamily member, light, contributes to optimal t-cell activation in vitro through co-stimulation of dendritic cell cytokine production; however, its role in t-cell-mediated control of intracellular bacterial infections is unknown. protective immunity against listeria monocytogenes and mycobacterium tuberculosis infection requires both antigen-specific cd4(+) and cd8(+) t cells. using light-deficient mice we determined that light was necessary for optimal re-stimulation of anti-listeri ... | 2010 | 20185431 |
| [acute encephalopathy, hydrocephalus and central diabetes insipidus as the first signs of listeria monocytogenes meningitis]. | 2010 | 20189898 | |
| molecular methods to assess listeria monocytogenes route of contamination in a dairy processing plant. | in this study we investigated the occurrence of listeria monocytogenes in a dairy processing plant during two sampling campaigns in 2007 and 2008. samples represented by semifinished and finished cheeses, swabs from the equipment and brines from the salting step, were subjected to analysis by using traditional and molecular methods, represented mainly by quantitative pcr. comparing the results obtained by the application of the two approaches used, it became evident how traditional microbiologic ... | 2010 | 20193970 |
| listeriolysin o-dependent bacterial entry into the cytoplasm is required for calpain activation and interleukin-1 alpha secretion in macrophages infected with listeria monocytogenes. | listeriolysin o (llo), an hly-encoded cytolysin of listeria monocytogenes, plays an essential role in the entry of l. monocytogenes into the host cell cytoplasm. l. monocytogenes-infected macrophages produce various proinflammatory cytokines, including interleukin-1 alpha (il-1 alpha), that contribute to the host immune response. in this study, we have examined il-1 alpha production in macrophages infected with wild-type l. monocytogenes or a nonescaping mutant strain deficient for llo (delta hl ... | 2010 | 20194588 |
| [neonatal sepsis caused by listeria monocytogenes: case report of 7 cases]. | 2010 | 20199739 | |
| listeriolysin o enhances cytoplasmic delivery by her-2 targeting liposomes. | to enhance cytoplasmic delivery of liposomal contents to breast cancer cells, the authors have attached the pore-forming protein, listeriolysin o (llo), to thermosensitive liposomes. the antibody trastuzumab (herceptin) was also conjugated with the outer surface of the liposomes, resulting in highly specific binding and internalization into mammary epithelial cells that overexpress the human epidermal growth factor receptor 2 (her-2). the liposomes were preloaded with a marker fluorescent dye, a ... | 2010 | 20201742 |
| incidence and contamination level of listeria monocytogenes and other listeria spp. in ready-to-eat meat products in jordan. | the objective of the present study was to investigate the incidence and contamination levels of different listeria monocytogenes serovars in ready-to-eat meat products (rte-mp) collected from different outlets and processing plants in jordan in order (i) to provide information to jordanian health authorities on the incidence of l. monocytogenes in rte-mp sold and consumed in jordan and (ii) to ascertain the risks of these products for consumers. two hundred forty rte-mp samples, 120 beef and 120 ... | 2010 | 20202341 |
| control of listeria monocytogenes on cooked cured ham by formulation with a lactate-diacetate blend and surface treatment with lauric arginate. | ready-to-eat (rte) meat products have been identified as a significant source of listeriosis in humans in the united states. meat processors in the united states are required to use one of three alternatives to control l. monocytogenes in rte meats: (i) a postlethality inactivation treatment along with a l. monocytogenes growth inhibitor; (ii) a postlethality inactivation treatment or a growth inhibitor; or (iii) sanitation measures and intensive testing. lauric arginate (lae) has been proposed ... | 2010 | 20202344 |
| estimating distributions out of qualitative and (semi)quantitative microbiological contamination data for use in risk assessment. | a framework using maximum likelihood estimation (mle) is used to fit a probability distribution to a set of qualitative (e.g., absence in 25 g), semi-quantitative (e.g., presence in 25 g and absence in 1g) and/or quantitative test results (e.g., 10 cfu/g). uncertainty about the parameters of the variability distribution is characterized through a non-parametric bootstrapping method. the resulting distribution function can be used as an input for a second order monte carlo simulation in quantitat ... | 2010 | 20202713 |
| rhombencephalitis caused by listeria monocytogenes in humans and ruminants: a zoonosis on the rise? | listeriosis is an emerging zoonotic infection of humans and ruminants worldwide caused by listeria monocytogenes (lm). in both host species, cns disease accounts for the high mortality associated with listeriosis and includes rhombencephalitis, whose neuropathology is strikingly similar in humans and ruminants. this review discusses the current knowledge about listeric encephalitis, and involved host and bacterial factors. there is an urgent need to study the molecular mechanisms of neuropathoge ... | 2010 | 20204066 |
| relative transcription of listeria monocytogenes virulence genes in liver pâtés with varying nacl content. | quantitative real time polymerase chain reaction (qrt pcr) was used to compare the relative transcription of prfa, inla, sigb and clpc for three listeria monocytogenes strains after incubation in i) a standard liver pâté versus brain heart infusion (bhi) broth and ii) the standard liver pâté versus three liver pâtés with reduced nacl content of which one also has been supplied with organic acids (ca-acetate and ca-lactate). the three strains (egd-e: reference strain; o57: more nacl sensitive; 68 ... | 2010 | 20206397 |
| photosensitization-based inactivation of food pathogen listeria monocytogenes in vitro and on the surface of packaging material. | the study was focused on the susceptibility of listeria monocytogenes atcl3c 7644 cells and biofilms to non-thermal antimicrobial treatment - photosensitization in vitro and after adhesion to the surface of packaging material. l. monocytogenes was incubated with 5-aminolevulinic acid (ala) (7.5 mm) for 0-2h and illuminated with visible light. the led-based light source used for the illumination emitted light lambda=400 nm with energy density 20 mw/cm(2). the illumination time varied 0-20 min, an ... | 2010 | 20207156 |
| evaluation of fermentation, drying, and/or high pressure processing on viability of listeria monocytogenes, escherichia coli o157:h7, salmonella spp., and trichinella spiralis in raw pork and genoa salami. | we evaluated the effectiveness of fermentation, drying, and high pressure processing (hpp) to inactivate listeria monocytogenes, escherichia coli o157:h7, salmonella spp., and trichinella spiralis in genoa salami produced with trichinae-infected pork. in addition, we evaluated the effectiveness of using hpp to inactivate t. spiralis larvae in pig masseter tissue. in part a, genoa salami batter (about 2.3 log larvae/g) prepared with trichinae-infected pork was separately spiked with a five-strain ... | 2010 | 20207436 |
| the growth and survival of food-borne pathogens in sweet and fermenting brewers' wort. | the aim of this study was to investigate the factors affecting the survival and growth of four food-borne pathogens (escherichia coli o157:h7, salmonella typhimurium, listeria monocytogenes, and staphylococcus aureus) in sweet and fermenting brewery wort. the gram-negative pathogens (e. coli and salm. typhimurium) were capable of growth during the initial stages of fermentation in hopped wort, although they were quickly inactivated when added during the later stages of fermentation. when the wor ... | 2010 | 20207437 |
| revelation by single-nucleotide polymorphism genotyping that mutations leading to a premature stop codon in inla are common among listeria monocytogenes isolates from ready-to-eat foods but not human listeriosis cases. | listeria monocytogenes utilizes internalin a (inla; encoded by inla) to cross the intestinal barrier to establish a systemic infection. multiple naturally occurring mutations leading to a premature stop codon (pmsc) in inla have been reported worldwide, and these mutations are causally associated with attenuated virulence. five inla pmsc mutations recently discovered among isolates from france and the united states were included as additional markers in our previously described inla single-nucle ... | 2010 | 20208021 |
| modeling the lag period and exponential growth of listeria monocytogenes under conditions of fluctuating temperature and water activity values. | the dynamic model for the growth of a bacterial population described by baranyi and roberts (j. baranyi and t. a. roberts, int. j. food microbiol. 23:277-294, 1994) was applied to model the lag period and exponential growth of listeria monocytogenes under conditions of fluctuating temperature and water activity (a(w)) values. to model the duration of the lag phase, the dependence of the parameter h(0), which quantifies the amount of work done during the lag period, on the previous and current en ... | 2010 | 20208022 |
| evaluation of immunomagnetic separation in combination with aloa listeria chromogenic agar for the isolation and identification of listeria monocytogenes in ready-to-eat foods. | a study was conducted to evaluate the performance of the aloa (chromogenic media) in combination with immunomagnetic separation (ims) for the detection of listeria monocytogenes in ready-to-eat food products. ims-aloa method was found to be equivalent to health canada's reference culture method as well as comparable to bax-pcr method in terms of the sensitivity of the methods for the detection of l. monocytogenes in ready-to-eat foods such as turkey roast, beef roast, mixed vegetable salads, pot ... | 2010 | 20211665 |
| ifn-gamma triggers ccr2-independent monocyte entry into the brain during systemic infection by virulent listeria monocytogenes. | listeria monocytogenes (lm) is a bacterial pathogen that infects the brain via parasitized monocytes. ccr2 is important for monocyte migration into the brain after it is infected, but the degree of ccr2 involvement in monocyte migration to the cns during systemic infection is less clear. our recent data demonstrate that systemic infection with non-neuroinvasive deltaacta lm mutants triggers ifn-gamma-dependent brain influxes of ly-6c(high) monocytes. studies presented here tested the extent to w ... | 2010 | 20211719 |
| listeria-derived acta is an effective adjuvant for primary and metastatic tumor immunotherapy. | tumor immunotherapy is currently at the cusp of becoming an important aspect of comprehensive cancer treatment in the clinic. however, the need for improved adjuvants to augment immune responses against tumor antigens is always present. in this paper, we characterize the listeria monocytogenes-derived actin-nucleating protein, acta, as a novel adjuvant for use in tumor immunotherapy. acta is a virulence factor that is expressed on the cell surface of l. monocytogenes and facilitates the producti ... | 2010 | 20213121 |
| listeria monocytogenes infection in patients with systemic lupus erythematosus. | listeria monocytogenes infection (lmi) is a rare complication in systemic lupus erythematosus (sle) patients, and it is associated with nonspecific clinical manifestations and is often mistaken with sle flares. several cases of lmi in sle patients have been reported, with high mortality rates. this article describes five new cases of lmi in patient with sle in a cohort of 174 patients (2.8%). all patients were women, with a mean age of 19.4 years (range, 5-29 years). mean duration of sle before ... | 2010 | 20213126 |
| enterococcus faecium isolated from honey synthesized bacteriocin-like substances active against different listeria monocytogenes strains. | four enterococcus faecium strains, isolated from honeycombs (c1 and m2d strains) and feral combs (mori1 and m1b strains) secreted antimicrobial substances active against fourteen different listeria spp. strains. the antimicrobial compound(s) present in the cell free supernatant were highly thermostable (121 degrees c for 15 min) and inactivated by proteolytic enzymes, but not by alpha-amylase and lipase, thus suggesting a peptidic nature. since the structural bacteriocin gene determinants of ent ... | 2010 | 20221729 |
| [prokaryotic expression of listeria monocytogene (lm) hly and development of monoclonal antibodies against listeriolysin o (llo)]. | in order to study the pathogenesis of listeria monocytogenes (lm), we cloned listeriolysin gene into prokaryotic expression vector pet21a. the expression vector was transformed into escherichia coli bl21 for expression of listeriolysin o (llo). llo-his tag fusion protein was purified with a ni-nta affinity column and was used as an immunogen to vaccinate balb/c mice. hybridomas were developed by fusing mouse myeloma cells sp2/0 and splenocytes from the immunized mice and screened with purified l ... | 2009 | 20222463 |
| effects of packaging type and storage temperature on the growth of foodborne pathogens on shredded 'romaine' lettuce. | fresh produce can be a vehicle for the transmission of pathogens capable of causing human illnesses and some of them can grow on fresh-cut vegetables. the survival and growth of escherichia coli o157:h7, salmonella spp. and listeria monocytogenes inoculated onto shredded lettuce was determined under modified atmosphere packaging conditions, at various storage temperatures. we also monitored changes in ph and gas atmospheres within the packages and the growth of psychrotrophic and mesophilic micr ... | 2010 | 20227602 |
| induction of tgf-beta1 and tgf-beta1-dependent predominant th17 differentiation by group a streptococcal infection. | recurrent group a streptococcus (gas) tonsillitis and associated autoimmune diseases indicate that the immune response to this organism can be ineffective and pathological. tgf-beta1 is recognized as an essential signal for generation of regulatory t cells (tregs) and t helper (th) 17 cells. here, the impact of tgf-beta1 induction on the t-cell response in mouse nasal-associated lymphoid tissue (nalt) following intranasal (i.n.) infections is investigated. elisa and tgf-beta1-luciferase reporter ... | 2010 | 20231435 |
| plasmid-associated bacteriocin production by lactobacillus lmg21688 suppresses listeria monocytogenes growth rebound in a food system. | bacteriocin produced by lactobacillus curvatus cwbi-b28wt is not completely effective against listeria monocytogenes in food models. there is evidence suggesting that bacteriocin-degrading proteolytic enzymes produced by the cwbi-b28wt strain and/or present in the food matrix contribute to this rebound of listeria growth. to limit this problem, we have partially characterized an approximately 10-kb plasmid responsible for bacteriocin production in l. curvatus cwbi-b28wt. this plasmid was transfe ... | 2010 | 20236328 |
| listeria and salmonella bacterial vectors of tumor-associated antigens for cancer immunotherapy. | this review covers the use of the facultative intracellular bacteria, listeriamonocytogenes and salmonella enterica serovar typhimurium as delivery systems for tumor-associated antigens in tumor immunotherapy. because of their ability to infect and survive in antigen presenting cells, these bacteria have been harnessed to deliver tumor antigens to the immune system both as bacterially expressed proteins and encoded on eukaryotic plasmids. they do this in the context of strong innate immunity, wh ... | 2010 | 20299242 |
| orthotopic liver transplantation: is there a risk for listeria monocytogenes infection? | immunosuppression of any kind is a known risk factor for infection with listeria monocytogenes (l. monocytogenes). particularly, patients with impaired liver function are at increased risk of developing an aggravated course of infection with this bacterial pathogen (see nolla-salas et al.; 2002 and cabellos et al.; 2008). it is a well-known pathogen in immunocompromised patients, but has only seldom been reported following orthotopic liver transplantation. invasion of the central nervous system ... | 2010 | 20300589 |
| implications of salt and sodium reduction on microbial food safety. | excess sodium consumption has been cited as a primary cause of hypertension and cardiovascular diseases. salt (sodium chloride) is considered the main source of sodium in the human diet, and it is estimated that processed foods and restaurant foods contribute 80% of the daily intake of sodium in most of the western world. however, ample research demonstrates the efficacy of sodium chloride against pathogenic and spoilage microorganisms in a variety of food systems. notable examples of the utilit ... | 2010 | 20301012 |
| dna probes for unambiguous identification of listeria monocytogenes epidemic clone ii strains. | listeria monocytogenes epidemic clone ii (ecii) strains have been responsible for two major multistate outbreaks of food-borne listeriosis in the united states, but their prevalence and ecology remain poorly understood. in this study, we describe dna probes that unambiguously identify this clonal group. these probes were able to differentiate ecii strains of outbreak, sporadic, or environmental origin from other l. monocytogenes strains of the same serotype (4b). | 2010 | 20305032 |
| cure of listeria monocytogenes meningitis after early transition to oral therapy. | 2010 | 20308389 | |
| histone acetylation at the single-cell level: a marker of memory cd8+ t cell differentiation and functionality. | following stimulation, memory t (t(m)) cells rapidly express many effector functions, a hallmark feature that allows them to provide protective immunity. recent studies suggest that genes involved in this rapid recall response may maintain an open chromatin structure in resting t(m) cells via epigenetic modifications. however, these studies have mostly focused on a few loci, and the techniques used required a large number of cells. we have developed a flow cytometric assay measuring histone modi ... | 2010 | 20308634 |
| listeria monocytogenes is sensed by the nlrp3 and aim2 inflammasome. | the inflammasome pathway functions to regulate caspase-1 activation in response to a broad range of stimuli. caspase-1 activation is required for the maturation of the pivotal pro-inflammatory cytokines of the pro-il-1beta family. in addition, caspase-1 activation leads to a certain type of cell death known as pyroptosis. activation of the inflammasome has been shown to play a critical role in the recognition and containment of various microbial pathogens, including the intracellularly replicati ... | 2010 | 20333626 |
| [preparation of a 96-microwell plate dna diagnostic chip for detection of foodborne bacteria and its application in an incident of food poisoning]. | to develop a 96-microwell plate dna diagnostic chip for simultaneous detection of 9 major foodborne bacteria. | 2010 | 20335099 |
| [listeria monocytogenes infection in a total knee arthroplasty]. | acute blood borne prosthetic infections occur in patients with an asymptomatic prosthesis that, after an infection involving bacteremia, produces bacterial implantation in the prosthesis and causes an acute infection. this type of infection is usually caused by gram positive cocci, streptococci and staphylococci. we present the clinical case of a patient without a history of immunodeficiency, who had an acute blood borne knee prosthetic infection caused by listeria monocytogenes. | 2009 | 20336882 |
| virulence factor p60 of listeria monocytogenes modulates innate immunity by inducing tumor necrosis factor alpha. | we investigated the effect of p60, a virulence factor of listeria monocytogenes, on host immune response in vitro and in vivo. administration of p60 before a sublethal infection with l. monocytogenes enhanced innate host resistance in naïve mice. mouse macrophage raw264.7 cells produced tumor necrosis factor (tnf)-alpha in response to stimulation with recombinant p60. toll-like receptor 4 may be involved in tnf-alpha production from raw264.7 cells and enhanced host resistance induced by p60 admi ... | 2010 | 20337701 |
| smcl as a novel diagnostic marker for quantitative detection of listeria ivanovii in biological samples. | to develop a novel molecular tool for the quantitative detection of the ruminant pathogen listeria ivanovii in different biological matrices. | 2010 | 20337766 |
| antibody-aptamer functionalized fibre-optic biosensor for specific detection of listeria monocytogenes from food. | to develop antibody-aptamer functionalized fibre-optic biosensor for specific detection of listeria monocytogenes from food products. | 2010 | 20337767 |
| global sensitivity analysis applied to a contamination assessment model of listeria monocytogenes in cold smoked salmon at consumption. | in this study, a variance-based global sensitivity analysis method was first applied to a contamination assessment model of listeria monocytogenes in cold smoked vacuum packed salmon at consumption. the impact of the choice of the modeling approach (populational or cellular) of the primary and secondary models as well as the effect of their associated input factors on the final contamination level was investigated. results provided a subset of important factors, including the food water activity ... | 2010 | 20345580 |
| experimental antibacterial therapy with puroindolines, lactoferrin and lysozyme in listeria monocytogenes-infected mice. | puroindoline a and puroindoline b from plant seeds, bovine lactoferrin and chicken eggs lysozyme are antimicrobial proteins of innate immune system that lyse invading organisms. we investigate their potential antibacterial activity against listeria monocytogenes in a mouse model. bacteria were isolated from various organs for 7 days after challenge. livers displayed consistently higher bacterial count (up to 10(7)cfu/g) than spleens, kidneys and brains. the efficacy of the amps was therefore est ... | 2010 | 20348006 |
| cooperative regulation of the induction of the novel antibacterial listericin by peptidoglycan recognition protein le and the jak-stat pathway. | intracellular bacteria cause serious infectious diseases such as tuberculosis, shigellosis, and listeriosis. the drosophila peptidoglycan recognition protein (pgrp)-le functions as an important host pattern recognition receptor against intracellular bacteria such as listeria monocytogenes. one pgrp-le-mediated intracellular response against l. monocytogenes infection is the induction of autophagy, a conserved intracellular degradation system. here, to further elucidate pgrp-le-mediated intracell ... | 2010 | 20348097 |
| risk of listeria monocytogenes contamination of raw ready-to-eat seafood products available at retail outlets in japan. | examination of listeria monocytogenes prevalence among ready-to-eat foods in japan revealed frequent (5.7 to 12.1%) contamination of minced tuna and fish roe products, and the isolates had the same virulence levels as clinical isolates in terms of invasion efficiency and infectivity in cell cultures and a murine infection model, respectively. premature stop codons in inla were infrequent (1 out of 39 isolates). cell numbers of l. monocytogenes in minced tuna and salmon roe increased rapidly unde ... | 2010 | 20348310 |
| poor invasion of trophoblastic cells but normal plaque formation in fibroblastic cells despite acta deletion in a group of listeria monocytogenes strains persisting in some food processing environments. | we determined mammalian cell invasion and virulence gene (inla, inlb, and acta) sequences of listeria monocytogenes strains belonging to a molecular subtype (rapd 9) that often persists in danish fish-processing plants. these strains invaded human placental trophoblasts less efficiently than other l. monocytogenes strains, including clinical strains, and they carry a premature stop codon in inla. eight of 15 strains, including the rapd 9 and maternofetal strains, had a 105-nucleotide deletion in ... | 2010 | 20348313 |
| alpha-galactosylceramide promotes killing of listeria monocytogenes within the macrophage phagosome through invariant nkt-cell activation. | alpha-galactosylceramide (alpha-galcer) has been exploited for the treatment of microbial infections. although amelioration of infection by alpha-galcer involves invariant natural killer t (inkt)-cell activation, it remains to be determined whether macrophages (mphi) participate in the control of microbial pathogens. in the present study, we examined the participation of mphi in immune intervention in infection by alpha-galcer using a murine model of listeriosis. phagocytic and bactericidal acti ... | 2010 | 20351146 |
| activation of naive nk cells in response to listeria monocytogenes requires il-18 and contact with infected dendritic cells. | the mechanisms for nk cell activation during infection by intracellular bacterial pathogens are not clearly defined. to dissect how listeria monocytogenes infection elicits nk cell activation, we evaluated the requirements for activation of naive splenic nk cells by infected bone marrow-derived dendritic cells (bmdcs). we found that nk cell activation in this setting required infection of bmdcs by live wild type bacteria. nk cells were not activated when bmdcs were infected with a live hemolysin ... | 2010 | 20351186 |
| the aim2 inflammasome is essential for host defense against cytosolic bacteria and dna viruses. | inflammasomes regulate the activity of caspase-1 and the maturation of interleukin 1beta (il-1beta) and il-18. aim2 has been shown to bind dna and engage the caspase-1-activating adaptor protein asc to form a caspase-1-activating inflammasome. using aim2-deficient mice, we identify a central role for aim2 in regulating caspase-1-dependent maturation of il-1beta and il-18, as well as pyroptosis, in response to synthetic double-stranded dna. aim2 was essential for inflammasome activation in respon ... | 2010 | 20351692 |
| inactivation of salmonella enterica and listeria monocytogenes inoculated on hydroponic tomatoes using chlorine dioxide gas. | the main objective of this study was to determine survivability of a cocktail of three strains of salmonella enterica (montevideo, javiana, and baildon) and two strains of listeria monocytogenes (lcdc 81-861 and f4244) on hydroponic tomatoes after treatment with chlorine dioxide (clo(2)) gas. an initial concentration of 8-9 log cfu/ml of salmonella and listeria cocktails was inoculated individually, in separate experiments, on tomato skin to obtain a population of 7-8 log cfu/cm(2) after drying ... | 2010 | 20353288 |
| microbial inhibitory and radical scavenging activities of cold-pressed terpeneless valencia orange (citrus sinensis) oil in different dispersing agents. | due to their low solubility in water, oil-based bioactive compounds require dispersion in a surface-active agent or appropriate solvents to ensure maximum contact with microorganisms. these combinations, however, may change their physical and/or chemical characteristics and consequently alter the desired functionality. the objective of this study was to determine the impact of selected dispersing agents, ethanol, dimethyl sulfoxide (dmso), and tween-80, on cold-pressed terpeneless (cpt) valencia ... | 2010 | 20355124 |
| internalin profiling and multilocus sequence typing suggest four listeria innocua subgroups with different evolutionary distances from listeria monocytogenes. | ecological, biochemical and genetic resemblance as well as clear differences of virulence between l. monocytogenes and l. innocua make this bacterial clade attractive as a model to examine evolution of pathogenicity. this study was attempted to examine the population structure of l. innocua and the microevolution in the l. innocua-l. monocytogenes clade via profiling of 37 internalin genes and multilocus sequence typing based on the sequences of 9 unlinked genes gyrb, sigb, dape, hisj, ribc, pur ... | 2010 | 20356375 |
| [listeria monocytogenes rhomboencephalitis. report of three cases]. | an unusual number of cases of rhomb encephalitis have occurred in chile because of the increased frequency of infections caused by listeria monocytogenes. we report three females aged 36, 40 and 55 years, with the disease. all presented with a prodrome characterized by headache, nausea, vomiting and fever, followed by ataxia and unilateral palsies of the third, seventh and twelfth cranial nerves. one patient presented also a hemi-hypoesthesia. magnetic resonance showed lesions in the posterior a ... | 2010 | 20361137 |
| culture-independent techniques applied to food industry water surveillance--a case study. | culture-independent techniques were used for the detection of pathogenic bacteria in drinking water at potentially critical control points along the production lines at a german dairy company and a spanish dry cured ham company. denaturing gradient gel electrophoresis (dgge) was used to describe bacterial population shifts indicating biological instability in the drinking water samples. autochthonous bacteria were identified by sequencing the excised dgge dna bands. more specifically, real-time ... | 2010 | 20363042 |
| prospects for new antimicrobials based on n,n-dimethylbiguanide complexes as effective agents on both planktonic and adhered microbial strains. | metal-free n,n-dimethylbiguanidium acetate and novel complexes m(dmbg)(2)(ch(3)coo)(2).nh(2)o (m: mn(ii), ni(ii), cu(ii) and zn(ii)) were screened for their antimicrobial properties against gram-positive (bacillus subtilis, listeria monocytogenes, staphylococcus aureus), gram-negative (klebsiella pneumoniae, escherichia coli, pseudomonas aeruginosa) bacteria and fungal (candida albicans) strains. the ability of compounds to inhibit the microbial adherence ability to the inert substratum as well ... | 2010 | 20363535 |
| single-cell techniques using chromosomally tagged fluorescent bacteria to study listeria monocytogenes infection processes. | listeria monocytogenes is a gram-positive facultative intracellular pathogen which invades different cell types, including nonphagocytic cells, where it is able to replicate and survive. the different steps of the cellular infectious process have been well described and consist of bacterial entry, lysis of the endocytic vacuole, intracellular replication, and spreading to neighboring cells. to study the listerial infectious process, gentamicin survival assays, plaque formation, and direct micros ... | 2010 | 20363781 |
| toll-like receptor 2- and myd88-dependent phosphatidylinositol 3-kinase and rac1 activation facilitates the phagocytosis of listeria monocytogenes by murine macrophages. | toll-like receptors (tlrs) play a key role in the innate immune response by sensing bacterial ligands. the mechanisms involved in the tlr-mediated cytokine response are well established; however, the possible contribution of tlr-dependent recognition of bacteria to macrophage phagocytosis remains unclear. listeria monocytogenes is an intracellular, parasitic, gram-positive bacterium recognized mainly by tlr2. in this study, we investigated whether tlr2-dependent signaling is involved in the phag ... | 2010 | 20368346 |
| antibacterial effect of the bioactive compound beauvericin produced by fusarium proliferatum on solid medium of wheat. | to obtain the bioactive compound beauvericin (bea), fusarium proliferatum cect 20569 was grown on a solid medium of wheat, utilizing the technique of the solid state fermentation (ssf), being this mycotoxin purified by high performance liquid chromatography (hplc) with a reverse phase semi-preparative column using as the mobile phase acetonitrile/water in gradient condition. the purity of the bea was verified by analytical hplc and liquid chromatography tandem mass spectrometry (lc/ms-ms). the p ... | 2010 | 20371252 |
| sumo, ubiquitin, ubl proteins: implications for human diseases - fifth international conference. | the fifth international conference on sumo, ubiquitin, ubl proteins: implications for human diseases, held in houston, included topics covering the latest advances and new targets in the field of protein modification. this conference report highlights selected presentations on the structural characterization of ubiquitination and sumoylation machinery; the regulation of ubiquitination enzymes, including e3 ligases; the functions and mechanism of action of sumo-targeted ubiquitin ligases (stubls) ... | 2010 | 20373248 |
| combined effect of enterocin as-48 and high hydrostatic pressure to control food-borne pathogens inoculated in low acid fermented sausages. | the single and combined effects of enterocin as-48 and high hydrostatic pressure (hhp) on listeria monocytogenes, salmonellaenterica, and staphylococcus aureus was investigated in fuet (a low acid fermented sausage) during ripening and storage at 7 degrees c or at room temperature. as-48 (148 au g(-1)) caused a drastic 5.5 log cfu g(-1) decrease in l. monocytogenes (p<0.001) and a significant (p<0.01) inhibition (1.79 logs) for salmonella at the end of ripening (10 d). after pressurization (400 ... | 2010 | 20374829 |
| control of listeria monocytogenes on commercially-produced frankfurters prepared with and without potassium lactate and sodium diacetate and surface treated with lauric arginate using the sprayed lethality in container (slic(r)) delivery method. | viability of listeriamonocytogenes was monitored on frankfurters formulated with or without potassium lactate and sodium diacetate at a ratio of ca. 7:1 and treated with lauric arginate (lae; 22 or 44ppm) using the sprayed lethality in container (slic(r)) delivery method. without antimicrobials, pathogen numbers remained relatively constant at ca. 3.3logcfu/package for ca. 30d, but then increased to ca. 8.4logcfu/package over 120d. regardless of whether or not lactate and diacetate were included ... | 2010 | 20374905 |
| dnase i inhibits a late phase of reactive oxygen species production in neutrophils. | neutrophils kill bacteria on extracellular complexes of dna fibers and bactericidal proteins known as neutrophil extracellular traps (nets). the net composition and the bactericidal mechanisms they use are not fully understood. here, we show that treatment with deoxyribonuclease (dnase i) impairs a late oxidative response elicited by gram-positive and gram-negative bacteria and also by phorbol ester. isoluminol-dependent chemiluminescence elicited by opsonized listeria monocytogenes-stimulated n ... | 2009 | 20375609 |
| food standards commission targets dangerous bacteria, chemicals. | 2009 | 20377055 | |
| protective immunity induced by a llo-deficient listeria monocytogenes. | listeria monocytogenes is a food-borne pathogen able to cause serious disease in human and animals. listeriolysin o (llo), a major virulence factor secreted by this bacterium, is a vacuole-specific lysin that facilitates bacterial entrance into the host cytosol. thus, llo plays a key role in the translocation and intracellular spread of l. monocytogenes. to study the effect of llo on virulence and immunopotency, a llo-deficient l. monocytogenes mutant was constructed using a shuttle vector follo ... | 2010 | 20377746 |
| a comparative risk assessment for listeria monocytogenes in prepackaged versus retail-sliced deli meat. | deli meat was ranked as the highest-risk ready-to-eat food vehicle of listeria monocytogenes within the 2003 u.s. food and drug administration and u.s. department of agriculture, food safety and inspection service risk assessment. the comparative risk of l. monocytogenes in retail-sliced versus prepackaged deli meats was evaluated with a modified version of this model. other research has found that retail-sliced deli meats have both higher prevalence and levels of l. monocytogenes than have prod ... | 2010 | 20377948 |
| quantitative risk assessment of listeriosis-associated deaths due to listeria monocytogenes contamination of deli meats originating from manufacture and retail. | the objective of this study was to estimate the relative risk of listeriosis-associated deaths attributable to listeria monocytogenes contamination in ham and turkey formulated without and with growth inhibitors (gis). two contamination scenarios were investigated: (i) prepackaged deli meats with contamination originating solely from manufacture at a frequency of 0.4% (based on reported data) and (ii) retail-sliced deli meats with contamination originating solely from retail at a frequency of 2. ... | 2010 | 20377949 |
| the combination of lactate and diacetate synergistically reduces cold growth in brain heart infusion broth across listeria monocytogenes lineages. | combinations of organic acids are often used in ready-to-eat foods to control the growth of listeria monocytogenes during refrigerated storage. the purpose of this study was to quantitatively assess synergy between two organic acid growth inhibitors under conditions similar to those present in cold-smoked salmon, and to assess the effect of evolutionary lineage on response to those growth inhibitors. thirteen strains of l. monocytogenes, representing lineages i and ii, were grown at 7 degrees c ... | 2010 | 20377950 |
| listeria monocytogenes contamination in pork can originate from farms. | the presence of listeria monocytogenes in the pork production chain was followed from farm to slaughterhouse by examining the farm and slaughterhouse levels in the same 364 pigs, and finally by analyzing the cut meats from the same pig lots. both organic and conventional farms were included in the study. altogether, 1,962 samples were collected, and the 424 l. monocytogenes isolates were analyzed by pulsed-field gel electrophoresis. the results from microbial analyses were combined with data fro ... | 2010 | 20377951 |
| use of ionic liquid-based extraction for recovery of salmonella typhimurium and listeria monocytogenes from food matrices. | methods for rapid separation (<5 h) and concentration of bacteria based on solubilization of complex food matrices have been developed recently to facilitate rapid molecular detection methods. however, a major disadvantage of these protocols is the resulting lack of viability of the microorganisms under study due to extensive use of chemicals and enzymes, which can inhibit subsequent quantitative microbiological analyses. in this study, a new class of organic salts, ionic liquids, were used for ... | 2010 | 20377956 |
| predicting growth-no growth of listeria monocytogenes on vacuum-packaged ready-to-eat meats. | compliance with u.s. department of agriculture (usda) composition-based labeling standards often has been regarded as evidence of the shelf stability of ready-to-eat (rte) meats. however, the usda now requires further proof of shelf stability. our previous work included development of equations for predicting the probability of staphylococcus aureus growth based on the ph and a(w) of an rte product. in the present study, we evaluated the growth-no-growth during 21 degrees c storage of listeria m ... | 2010 | 20377960 |
| alteration of the phospho- or neutral lipid content and fatty acid composition in listeria monocytogenes due to acid adaptation mechanisms for hydrochloric, acetic and lactic acids at ph 5.5 or benzoic acid at neutral ph. | this study provides a first approach to observe the effects on listeria monocytogenes of cellular exposure to acid stress at low or neutral ph, notably how phospho- or neutral lipids are involved in this mechanism, besides the fatty acid profile alteration. a thorough investigation of the composition of polar and neutral lipids from l. monocytogenes grown at ph 5.5 in presence of hydrochloric, acetic and lactic acids, or at neutral ph 7.3 in presence of benzoic acid, is described relative to cel ... | 2010 | 20379849 |
| molecular basis for peptidoglycan recognition by a bactericidal lectin. | regiii proteins are secreted c-type lectins that kill gram-positive bacteria and play a vital role in antimicrobial protection of the mammalian gut. regiii proteins bind their bacterial targets via interactions with cell wall peptidoglycan but lack the canonical sequences that support calcium-dependent carbohydrate binding in other c-type lectins. here, we use nmr spectroscopy to determine the molecular basis for peptidoglycan recognition by hip/pap, a human regiii lectin. we show that hip/pap r ... | 2010 | 20382864 |
| effectiveness of low concentration electrolyzed water to inactivate foodborne pathogens under different environmental conditions. | strong acid electrolyzed water (saew) has a very limited application due to its low ph value (<2.7) and corrosive characteristics. thus, we developed new low concentration electrolyzed water (lcew). the efficacy of lcew under various treatment conditions for the inactivation of different foodborne pathogens in pure culture was evaluated and compared with saew. the efficiency of lcew and saew for the inactivation of predominant foodborne pathogens (escherichia coli o157:h7, listeria monocytogenes ... | 2010 | 20385418 |
| caspase recruitment domain-containing protein 8 (card8) negatively regulates nod2-mediated signaling. | caspase activating and recruitment domain 8 (card8) has been implicated as a co-regulator of several pro-inflammatory and apoptotic signaling pathways. in the present study, we demonstrate a specific modulation of nod2-induced signaling by card8 in intestinal epithelial cells. we show that card8 physically interacts with nod2 and inhibits nodosome assembly and subsequent signaling upon muramyl-dipeptide stimulation. furthermore, card8 inhibits the direct bactericidal effect of nod2 against intra ... | 2010 | 20385562 |
| cellular pharmacodynamics of the novel biaryloxazolidinone radezolid: studies with infected phagocytic and nonphagocytic cells, using staphylococcus aureus, staphylococcus epidermidis, listeria monocytogenes, and legionella pneumophila. | radezolid is a novel biaryloxazolidinone in clinical development which shows improved activity, including against linezolid-resistant strains. in a companion paper (29), we showed that radezolid accumulates about 11-fold in phagocytic cells, with approximately 60% of the drug localized in the cytosol and approximately 40% in the lysosomes of the cells. the present study examines its activity against (i) bacteria infecting human thp-1 macrophages and located in different subcellular compartments ... | 2010 | 20385852 |
| experimental conditions that enhance potency of an antibacterial oligo-acyl-lysyl. | oligo-acyl-lysyls (oaks) are synthetic mimics of host defense peptides known to exert antibacterial activity both in cultures and in animal models of disease. here, we investigated how environmental conditions (temperature, ph, and ionic strength) affect the antibacterial properties of an octamer derivative, c(12)k-7alpha(8). data obtained with representative bacteria, including the gram-negative bacterium escherichia coli and the gram-positive bacteria listeria monocytogenes and staphylococcus ... | 2010 | 20385856 |
| antimicrobial resistance of listeria monocytogenes strains isolated from humans in france. | susceptibility to antibiotics of 4,816 clinical l. monocytogenes strains isolated since 1926 was studied, and the temporal evolution of susceptibility to antibiotics was analyzed through several decades. the mechanisms of resistance in each resistant strain were studied. the prevalence of resistant strains was estimated at 1.27% among isolates from humans. resistance to tetracyclines+ and fluoroquinolones was more common and has recently emerged. although acquired resistance in clinical l. monoc ... | 2010 | 20385859 |