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regulation of gene expression patterns in mosquito reproduction.in multicellular organisms, development, growth and reproduction require coordinated expression of numerous functional and regulatory genes. insects, in addition to being the most speciose animal group with enormous biological and economical significance, represent outstanding model organisms for studying regulation of synchronized gene expression due to their rapid development and reproduction. disease-transmitting female mosquitoes have adapted uniquely for ingestion and utilization of the hug ...201526274815
meteorologically driven simulations of dengue epidemics in san juan, pr.meteorological factors influence dengue virus ecology by modulating vector mosquito population dynamics, viral replication, and transmission. dynamic modeling techniques can be used to examine how interactions among meteorological variables, vectors and the dengue virus influence transmission. we developed a dengue fever simulation model by coupling a dynamic simulation model for aedes aegypti, the primary mosquito vector for dengue, with a basic epidemiological susceptible-exposed-infectious-re ...201526275146
heme signaling impacts global gene expression, immunity and dengue virus infectivity in aedes aegypti.blood-feeding mosquitoes are exposed to high levels of heme, the product of hemoglobin degradation. heme is a pro-oxidant that influences a variety of cellular processes. we performed a global analysis of heme-regulated aedes aegypti (yellow fever mosquito) transcriptional changes to better understand influence on mosquito physiology at the molecular level. we observed an iron- and reactive oxygen species (ros)-independent signaling induced by heme that comprised genes related to redox metabolis ...201526275150
development of resistance to pyrethroid in culex pipiens pallens population under different insecticide selection pressures.current vector control programs are largely dependent on pyrethroids, which are the most commonly used and only insecticides recommended by the world health organization for insecticide-treated nets (itns). however, the rapid spread of pyrethroid resistance worldwide compromises the effectiveness of control programs and threatens public health. since few new insecticide classes for vector control are anticipated, limiting the development of resistance is crucial for prolonging efficacy of pyreth ...201526275298
energy homeostasis control in drosophila adipokinetic hormone mutants.maintenance of biological functions under negative energy balance depends on mobilization of storage lipids and carbohydrates in animals. in mammals, glucagon and glucocorticoid signaling mobilizes energy reserves, whereas adipokinetic hormones (akhs) play a homologous role in insects. numerous studies based on akh injections and correlative studies in a broad range of insect species established the view that akh acts as master regulator of energy mobilization during development, reproduction, a ...201526275422
role of aedes aegypti and aedes albopictus during the 2011 dengue fever epidemics in hanoi, vietnam.to record the human cases of dengue fever (df) and investigate the aedes mosquito species circulating during the hanoi 2011 df epidemics.201526276285
green synthesis of silver nanoparticles from cassia roxburghii-a most potent power for mosquito control.mosquitoes transmit serious human diseases, causing millions of deaths every year. the use of synthetic insecticides to control vector mosquitoes has caused physiological resistance and adverse environmental effects in addition to high operational cost. insecticides of synthesized natural products for vector control have been a priority in this area. in the present study, silver nanoparticles (agnps) synthesized using cassia roxburghii plant leaf extract against anopheles stephensi, aedes aegypt ...201526276645
physiological and biochemical effects of botanical extract from piper nigrum linn (piperaceae) against the dengue vector aedes aegypti liston (diptera: culicidae).the leaves of piper nigrum l. (piperaceae) were evaluated for chemical constituents and mosquito larvicidal activity against the larvae of aedes aegypti. gc and gc-ms analyses revealed that the crude extracts contain 16 compounds. thymol (20.77%) and ç-elemene (10.42%) were identified as the major constituents followed by cyclohexene, 4-ethenyl-4-methyl-3-(1-methylethenyl)-1-(1 methylethyl)-, (3r-trans) (7.58%), 4,6-octadienoic acid, 2-acetyl-2-methyl-, ethyl ester (6.98), 2(3h)-furanone, 3,4-bi ...201526277727
next-generation sequencing in clinical virology: discovery of new viruses.viruses are a cause of significant health problem worldwide, especially in the developing nations. due to different anthropological activities, human populations are exposed to different viral pathogens, many of which emerge as outbreaks. in such situations, discovery of novel viruses is utmost important for deciding prevention and treatment strategies. since last century, a number of different virus discovery methods, based on cell culture inoculation, sequence-independent pcr have been used fo ...026279987
s argassum muticum-synthesized silver nanoparticles: an effective control tool against mosquito vectors and bacterial pathogens.mosquito-borne diseases represent a deadly threat for millions of people worldwide. furthermore, pathogens and parasites polluting water also constitute a severe plague for populations of developing countries. in this research, silver nanoparticles (agnp) were synthesized using the aqueous extract of the seaweed sargassum muticum. the production of agnp was confirmed by surface plasmon resonance band illustrated in uv-vis spectrophotometry. agnp were characterized by ftir, sem, edx, and xrd anal ...201526281786
anopheles stephensi p38 mapk signaling regulates innate immunity and bioenergetics during plasmodium falciparum infection.fruit flies and mammals protect themselves against infection by mounting immune and metabolic responses that must be balanced against the metabolic needs of the pathogens. in this context, p38 mitogen-activated protein kinase (mapk)-dependent signaling is critical to regulating both innate immunity and metabolism during infection. accordingly, we asked to what extent the asian malaria mosquito anopheles stephensi utilizes p38 mapk signaling during infection with the human malaria parasite plasmo ...201526283222
pirnas derived from ancient viral processed pseudogenes as transgenerational sequence-specific immune memory in mammals.endogenous bornavirus-like nucleoprotein elements (eblns) are sequences within vertebrate genomes derived from reverse transcription and integration of ancient bornaviral nucleoprotein mrna via the host retrotransposon machinery. while species with eblns appear relatively resistant to bornaviral disease, the nature of this association is unclear. we hypothesized that eblns could give rise to antiviral interfering rna in the form of piwi-interacting rnas (pirnas), a class of small rna known to si ...201526283688
venus kinase receptors at the crossroads of insulin signaling: their role in reproduction for helminths and insects.venus kinase receptors (vkrs) are invertebrate receptor tyrosine kinases (tks) first discovered in the human parasite schistosoma. they contain an extracellular venus flytrap module similar to the ligand-binding domain of g protein-coupled receptors of class c and an intracellular tk domain similar to that of insulin receptors. vkrs are present from cnidarians to echinoderms. they were shown to be activated by amino-acids, to induce insulin-like intracellular pathways, and to be highly expressed ...201526284029
rapid biosynthesis of silver nanoparticles using crotalaria verrucosa leaves against the dengue vector aedes aegypti: what happens around? an analysis of dragonfly predatory behaviour after exposure at ultra-low doses.aedes aegypti is a primary vector of dengue, a mosquito-borne viral disease infecting 50-100 million people every year. here, we biosynthesised mosquitocidal silver nanoparticles (agnp) using the aqueous leaf extract of crotalaria verrucosa. the green synthesis of agnp was studied by uv-vis spectroscopy, sem, edx and ftir. c. verrucosa-synthesised agnps were toxic against a. aegypti larvae and pupae. lc50 of agnp ranged from 3.496 ppm (i instar larvae) to 17.700 ppm (pupae). furthermore, we eval ...201626284510
chitosan: an update on potential biomedical and pharmaceutical applications.chitosan is a natural polycationic linear polysaccharide derived from chitin. the low solubility of chitosan in neutral and alkaline solution limits its application. nevertheless, chemical modification into composites or hydrogels brings to it new functional properties for different applications. chitosans are recognized as versatile biomaterials because of their non-toxicity, low allergenicity, biocompatibility and biodegradability. this review presents the recent research, trends and prospects ...201526287217
wolbachia do not induce reactive oxygen species-dependent immune pathway activation in aedes albopictus.aedes albopictus is a major vector of dengue (denv) and chikungunya (chikv) viruses, causing millions of infections annually. it naturally carries, at high frequency, the intracellular inherited bacterial endosymbiont wolbachia strains walba and walbb; transinfection with the higher-density wolbachia strain wmel from drosophila melanogaster led to transmission blocking of both arboviruses. the hypothesis that reactive oxygen species (ros)-induced immune activation plays a role in arbovirus inhib ...201526287231
oxidative stress and maxi calcium-activated potassium (bk) channels.all cells contain ion channels in their outer (plasma) and inner (organelle) membranes. ion channels, similar to other proteins, are targets of oxidative impact, which modulates ion fluxes across membranes. subsequently, these ion currents affect electrical excitability, such as action potential discharge (in neurons, muscle, and receptor cells), alteration of the membrane resting potential, synaptic transmission, hormone secretion, muscle contraction or coordination of the cell cycle. in this c ...201526287261
clinical and laboratory profile of dengue in the elderly.dengue is the most rapidly spreading mosquito-borne viral disease in the world with a 30-fold increase in incidence in the last 50 years. approximately, 50 million dengue infections occur annually.026288776
nanoparticles in the fight against mosquito-borne diseases: bioactivity of bruguiera cylindrica-synthesized nanoparticles against dengue virus den-2 (in vitro) and its mosquito vector aedes aegypti (diptera: culicidae).mosquitoes are blood-feeding insects serving as the most important vectors for spreading human pathogens and parasites. dengue is a viral disease mainly vectored through the bite of aedes mosquitoes. its transmission has recently increased in urban and semi-urban areas of tropical and subtropical regions worldwide, becoming a major international public health concern. there is no specific treatment for dengue. its prevention and control solely depend on effective vector control measures. mangrov ...201526290219
chitosan, carbon quantum dot, and silica nanoparticle mediated dsrna delivery for gene silencing in aedes aegypti: a comparative analysis.in spite of devastating impact of mosquito borne pathogens on humans, widespread resistance to chemical insecticides and environmental concerns from residual toxicity limit mosquito control strategies. we tested three nanoparticles, chitosan, carbon quantum dot (cqd), and silica complexed with dsrna, to target two mosquito genes (snf7 and src) for controlling aedes aegypti larvae. relative mrna levels were quantified using qrt-pcr to evaluate knockdown efficiency in nanoparticle-dsrna treated la ...201526291176
reemergence and autochthonous transmission of dengue virus, eastern china, 2014.in 2014, 20 dengue cases were reported in the cities of wenzhou (5 cases) and wuhan (15 cases), china, where dengue has rarely been reported. dengue virus 1 was detected in 4 patients. although most of these cases were likely imported, epidemiologic analysis provided evidence for autochthonous transmission.026292098
modelling, simulation and social network data: what's new for public health and epidemiology informatics? 026293870
traditional uses, phytochemistry, and bioactivities of cananga odorata (ylang-ylang).ylang-ylang (cananga odorata hook. f. & thomson) is one of the plants that are exploited at a large scale for its essential oil which is an important raw material for the fragrance industry. the essential oils extracted via steam distillation from the plant have been used mainly in cosmetic industry but also in food industry. traditionally, c. odorata is used to treat malaria, stomach ailments, asthma, gout, and rheumatism. the essential oils or ylang-ylang oil is used in aromatherapy and is bel ...201526294929
utilization of biodiesel by-products for mosquito control.the current paper has elaborated the efficient utilization of non-edible oil seed cakes (neoc), by-products of the bio-diesel extraction process to develop a herbal and novel mosquitocidal composition against the aedes aegypti larvae. the composition consisted of botanical active ingredients, inerts, burning agents and preservatives; where the botanical active ingredients were karanja (pongamia glabra) cake powder and jatropha (jatropha curcas) cake powder, products left after the extraction of ...201626296531
widespread evidence for interspecific mating between aedes aegypti and aedes albopictus (diptera: culicidae) in nature.aedes aegypti and aedes albopictus, two important vectors of the dengue and chikungunya viruses to humans, often come in contact in their invasive ranges. in these circumstances, a number of factors are thought to influence their population dynamics, including resource competition among the larval stages, prevailing environmental conditions and reproductive interference in the form of satyrization. as the distribution and abundance of ae. aegypti and ae. albopictus have profound epidemiological ...201526296606
identification and evaluation of vaccine candidate antigens from the poultry red mite (dermanyssus gallinae).an aqueous extract of the haematophagous poultry ectoparasite, dermanyssus gallinae, was subfractionated using anion exchange chromatography. six of these subfractions were used to immunise hens and the blood from these hens was fed, in vitro, to poultry red mites. mite mortality following these feeds was indicative of protective antigens in two of the subfractions, with the risks of mites dying being 3.1 and 3.7 times higher than in the control group (p<0.001). a combination of two-dimensional ...201526296690
further evidences for the mode of action of the larvicidal m-pentadecadienyl-phenol isolated from myracrodruon urundeuva seeds against aedes aegypti.nowadays, dengue fever is considered the most important arbovirosis worldwide and its control is still based upon combating the vector aedes aegypti. besides monitoring of mosquito populations resistant to conventional insecticides, the search for new environmentally safe insecticides and conduction of molecular studies focusing on the elucidation of mode of action and possible resistance mechanisms are considered the key for a sustainable management of the mosquito vector. thus, the present wor ...201526299195
genome analysis and polar tube firing dynamics of mosquito-infecting microsporidia.microsporidia are highly divergent fungi that are obligate intracellular pathogens of a wide range of host organisms. here we review recent findings from the genome sequences of mosquito-infecting microsporidian species edhazardia aedis and vavraia culicis, which show large differences in genome size, although similar numbers of predicted genes. we also show a video of e. aedis polar tube firing, which is the dramatic mechanism used by microsporidia to deliver the germ cell (sporoplasm) into the ...201526300319
eco-friendly control of malaria and arbovirus vectors using the mosquitofish gambusia affinis and ultra-low dosages of mimusops elengi-synthesized silver nanoparticles: towards an integrative approach?mosquito-borne diseases represent a deadly threat for millions of people worldwide. however, the use of synthetic insecticides to control culicidae may lead to high operational costs and adverse non-target effects. plant-borne compounds have been proposed for rapid extracellular synthesis of mosquitocidal nanoparticles. their impact against biological control agents of mosquito larval populations has been poorly studied. we synthesized silver nanoparticles (agnp) using the aqueous leaf extract o ...201526300364
β carbonic anhydrase is required for female fertility in drosophila melanogaster.carbonic anhydrases (cas, ec 4.2.1.1) are ubiquitous enzymes that catalyze the reversible hydration reaction of carbon dioxide. cas are present as six structurally divergent enzyme families: α, β, γ, δ, ζ and η. β-cas have a wide distribution across different species including invertebrates. previously, we showed that drosophila melanogaster β-ca is a highly active mitochondrial enzyme. in this study, we investigated the function of drosophila β-ca by silencing the expression of the β-ca gene us ...201526300950
building better environmental risk assessments.risk assessment is a reasoned, structured approach to address uncertainty based on scientific and technical evidence. it forms the foundation for regulatory decision-making, which is bound by legislative and policy requirements, as well as the need for making timely decisions using available resources. in order to be most useful, environmental risk assessments (eras) for genetically modified (gm) crops should provide consistent, reliable, and transparent results across all types of gm crops, tra ...201526301217
source and purity of dengue-viral preparations impact requirement for enhancing antibody to induce elevated il-1β secretion: a primary human monocyte model.dengue virus is a major global health threat and can lead to life-threatening hemorrhagic complications due to immune activation and cytokine production. cross-reactive antibodies to an earlier dengue virus infection are a recognized risk factor for severe disease. these antibodies bind heterologous dengue serotypes and enhance infection into fc-receptor-bearing cells, a process known as antibody-dependent enhancement of infection. one crucial cytokine seen elevated in severe dengue patients is ...201526301593
[control of aedes aegypti breeding sites with the program recicla por tu bienestar in merida, mexico].to determine the importance of ae. aegypti breeding-sites in merida;to evaluate the impact of recicla por tu bienestar (rxb, a recycling program) on the reduction of breeding sites and the perception of participants.201726302122
mercadeo virus: a novel mosquito-specific flavivirus from panama.viruses in the genus flavivirus (family flaviviridae) include many arthropod-borne viruses of public health and veterinary importance. however, during the past two decades an explosion of novel insect-specific flaviviruses (isfs), some closely related to vertebrate pathogens, have been discovered. although many flavivirus pathogens of vertebrates have been isolated from naturally infected mosquitoes in panama, isfs have not previously been reported from the country. this report describes the iso ...201526304915
surveillance potential of non-native hawaiian birds for detection of west nile virus.west nile virus (wnv) was first detected in north america in 1999. alaska and hawaii (hi) remain the only u.s. states in which transmission of wnv has not been detected. dead bird surveillance has played an important role in the detection of the virus geographically, as well as temporally. in north america, corvids have played a major role in wnv surveillance; however, the only corvid in hi is the endangered hawaiian crow that exists only in captivity, thus precluding the use of this species for ...201526304918
patterns of flavivirus seroprevalence in the human population of northern laos.a total of 1,136 samples from 289 households in four provinces in northern laos were subjected to japanese encephalitis virus (jev) and dengue virus hemagglutination inhibition (denv hi). overall, antibodies to jev were detected by hi in 620 (54.6%) of 1,136 people; of which 217 (19.1%) had hi activity against jev only. antibodies to denv4 were detected by hi in 526 (46.3%) of 1,136 people; of which 124 (10.9%) had hi activity against denv4 only. antibodies to denv1-3 were detected by hi in 296 ...201526304925
a catalog of proteins expressed in the ag secreted fluid during the mature phase of the chinese mitten crabs (eriocheir sinensis).the accessory gland (ag) is an important component of the male reproductive system of arthropods, its secretions enhance fertility, some ag proteins bind to the spermatozoa and affect its function and properties. here we report the first comprehensive catalog of the ag secreted fluid during the mature phase of the chinese mitten crab (eriocheir sinensis). ag proteins were separated by one-dimensional gel electrophoresis and analyzed by reverse phase high-performance liquid chromatography coupled ...201526305468
a potent neutralizing igm mab targeting the n218 epitope on e2 protein protects against chikungunya virus pathogenesis.chikungunya virus (chikv) is a medically important human viral pathogen that causes chikungunya fever accompanied with debilitating and persistent joint pain. host-elicited or passively-transferred monoclonal antibodies (mab) are essential mediators of chikv clearance. therefore, this study aimed to generate and characterize a panel of mabs for their neutralization efficacy against chikv infection in a cell-based and murine model. to evaluate their antigenicity and neutralization profile, indire ...201526305993
risk factors associated with asthma, atopic dermatitis and rhinoconjunctivitis in a rural senegalese cohort.the world allergy organization estimates that 40 % of the world's population is affected by allergic diseases. the international study of asthma and allergies in childhood has completed phase iii and it has now become clear that these diseases have increased in developing countries, especially africa, where prevalence rates were formerly low. despite an increase in studies in africa, few sub-saharan west african countries are represented; the focus has remained on urban populations and little at ...201526306096
de novo transcriptome assembly and analysis to identify potential gene targets for rnai-mediated control of the tomato leafminer (tuta absoluta).providing double-stranded rna (dsrna) to insects has been proven to silence target genes, and this approach has emerged as a potential method to control agricultural pests by engineering plants to express insect dsrnas. a critical step of this technology is the screening of effective target genes essential for insect development and/or survival. the tomato leafminer (tuta absoluta meyrick) is a major solanum lycopersicum (tomato) pest that causes significant yield losses and has recently invaded ...201526306628
a novel olfactory pathway is essential for fast and efficient blood-feeding in mosquitoes.in mosquitoes, precise and efficient finding of a host animal is crucial for survival. one of the poorly understood aspects of mosquito blood-feeding behavior is how these insects target an optimal site in order to penetrate the skin and blood vessels without alerting the host animal. here we provide new findings that a piercing structure of the mouthpart of the mosquitoes, the stylet, is an essential apparatus for the stage in blood feeding. indeed, the stylet possesses a number of sensory hair ...201526306800
the waal gene mutation compromised the inhabitation of enterobacter sp. ag1 in the mosquito gut environment.the mosquito gut harbors a variety of bacteria that are dynamically associated with mosquitoes in various contexts. however, little is known about bacterial factors that affect bacterial inhabitation in the gut microbial community. enterobacter sp. ag1 is a predominant gram negative bacterium in the mosquito midgut.201526306887
susceptibility profile of aedes aegypti from santiago island, cabo verde, to insecticides.in 2009, cabo verde diagnosed the first dengue cases, with 21,137 cases reported and aedes aegypti was identified as the vector. since the outbreak, chemical insecticides and source reduction were used to control the mosquito population. this study aimed to assess the susceptibility of a. aegypti populations from santiago, cabo verde to insecticides and identify the mechanisms of resistance. samples of a. aegypti eggs were obtained at two different time periods (2012 and 2014), using ovitraps in ...201526307496
inhibitory effects of amorphigenin on the mitochondrial complex i of culex pipiens pallens coquillett (diptera: culicidae).previous studies in our laboratory found that the extract from seeds of amorpha fruticosa in the leguminosae family had lethal effects against mosquito larvae, and an insecticidal compound amorphigenin was isolated. in this study, the inhibitory effects of amorphigenin against the mitochondrial complex i of culex pipiens pallens (diptera: culicidae) were investigated and compared with that of rotenone. the results showed that amorphigenin and rotenone can decrease the mitochondrial complex i act ...201526307964
antimicrobial and antileishmanial activities of diterpenoids isolated from the roots of salvia deserta.four diterpenes with biological activity were isolated from salvia deserta roots. taxodione was considered leishmanicidal with an ic50 value of 1.46 µm (0.46 mg/l) against leishmania donovani and also exhibited antifungal and antimicrobial activities. ferruginol displayed the greatest activity [24-h ic50 of 4.5 µm (1.29 mg/l)] against the fish pathogenic bacteria streptococcus iniae. the crude extract fraction that contained the isolated compounds 7-o-acetylhorminone and horminone showed stronge ...201626308356
comparative behavioral responses of pyrethroid-susceptible and -resistant aedes aegypti (diptera: culicidae) populations to citronella and eucalyptus oils.the objective of this study was to compare the behavioral responses (contact irritancy and noncontact spatial repellency) between susceptible and resistant populations of aedes aegypti (l.) (=stegomyia aegypti) to essential oils, citronella, and eucalyptus, eucalyptus globulus, extracts, using an excito-repellency test system. n, n-diethyl-meta-toluamide (deet) was used as the standard reference repellent. mosquitoes included two long-standing insecticide susceptible colonies (u.s. department of ...201426309305
inheritance of resistance to deltamethrin in aedes aegypti (diptera: culicidae) from cuba.the development of pyrethroid resistance in aedes aegypti (l) (diptera: culicidae) is a serious concern because major a. aegypti control programs are predominantly based on pyrethroid use during epidemic disease outbreaks. research about the genetic basis for pyrethroid resistance and how it is transmitted among mosquito populations is needed. the objective of this study was to determine how deltamethrin resistance is inherited in the cuban a. aegypti-resistant reference strain. here, a field po ...201426309309
protection from mosquito biting provided by permethrin-treated military fabrics.a study to evaluate the protection provided by permethrin-treated fabric following cold-water washing against biting by mosquitoes is reported. australian defense force (adf) disruptive pattern combat uniform (dpcu) shirt fabric and entire shirts were treated by dipping in a 0.6% emulsion (perigen defense, containing 500 g/liter permethrin), and commercial factory treatment in the united states (factory a) and europe (factory b). protection was recorded after 1, 3, 5, 10, 30, and 50 washes. the ...201426309310
influence of time of assay on behavioral responses of laboratory and field populations aedes aegypti and culex quinquefasciatus (diptera: culicidae) to deet.knowledge on test conditions that may influence behavioral responses of mosquitoes is critical when excito-repellency tests are conducted. the objective of this study was to investigate the effect of test time differences on normal circadian activity and behavioral responses of field and colonized aedes aegypti (l.) (=stegomyia aegypti) and culex quinquefasciatus say to deet, one of the most common synthetic repellent active ingredients available. two field populations of ae. aegypti and cx. qui ...201426309311
vector competence of selected mosquito species in kenya for ngari and bunyamwera viruses.bunyamwera and ngari viruses have been isolated from a range of mosquito species in kenya but their actual role in the maintenance and transmission of these viruses in nature remains unclear. identification of the mosquito species efficient in transmitting these viruses is critical for estimating the risk of human exposure and understanding the transmission and maintenance mechanism. we determined the vector competence of, aedes aegypti (l.), culex quinquefasciatus say, and anopheles gambiae gil ...201426309314
effective disposal of nitrogen waste in blood-fed aedes aegypti mosquitoes requires alanine aminotransferase.to better understand the mechanisms responsible for the success of female mosquitoes in their disposal of excess nitrogen, we investigated the role of alanine aminotransferase (alat) in blood-fed aedes aegypti. transcript and protein levels from the 2 alat genes were analyzed in sucrose- and blood-fed a. aegypti tissues. alat1 and alat2 exhibit distinct expression patterns in tissues during the first gonotrophic cycle. injection of female mosquitoes with either double-stranded rna (dsrna)-alat1 ...201526310269
immunogenicity and serological cross-reactivity of saliva proteins among different tsetse species.tsetse are vectors of pathogenic trypanosomes, agents of human and animal trypanosomiasis in africa. components of tsetse saliva (sialome) are introduced into the mammalian host bite site during the blood feeding process and are important for tsetse's ability to feed efficiently, but can also influence disease transmission and serve as biomarkers for host exposure. we compared the sialome components from four tsetse species in two subgenera: subgenus morsitans: glossina morsitans morsitans (gmm) ...201526313460
molecular identification of saint louis encephalitis virus genotype iv in colombia.saint louis encephalitis virus (slev) is a member of the japanese-encephalitis virus serocomplex of the genus flavivirus. slev is broadly distributed in the americas and the caribbean islands, where it is usually transmitted by mosquitoes of the genus culex and primarily to birds and mammalian-hosts. humans are occasionally infected by the virus and are dead-end hosts. slev causes encephalitis in temperate regions, while in tropical regions of the americas, several human cases and a wide biologi ...201526313538
involvement of antizyme characterized from the small abalone haliotis diversicolor in gonadal development.the small abalone haliotis diversicolor is an economically important mollusk that is widely cultivated in southern china. gonad precocity may affect the aquaculture of small abalone. polyamines, which are small cationic molecules essential for cellular proliferation, may affect gonadal development. ornithine decarboxylase (odc) and antizyme (az) are essential elements of a feedback circuit that regulates cellular polyamines. this paper presents the molecular cloning and characterization of az fr ...201526313647
role of marine natural products in the genesis of antiviral agents. 201526317854
increasing dengue incidence in singapore over the past 40 years: population growth, climate and mobility.in singapore, the frequency and magnitude of dengue epidemics have increased significantly over the past 40 years. it is important to understand the main drivers for the rapid increase in dengue incidence. we studied the relative contributions of putative drivers for the rise of dengue in singapore: population growth, climate parameters and international air passenger arrivals from dengue endemic countries, for the time period of 1974 until 2011. we used multivariable poisson regression models w ...201526322517
electrostatic coating enhances bioavailability of insecticides and breaks pyrethroid resistance in mosquitoes.insecticide resistance poses a significant and increasing threat to the control of malaria and other mosquito-borne diseases. we present a novel method of insecticide application based on netting treated with an electrostatic coating that binds insecticidal particles through polarity. electrostatic netting can hold small amounts of insecticides effectively and results in enhanced bioavailability upon contact by the insect. six pyrethroid-resistant anopheles mosquito strains from across africa we ...201526324912
tracking dengue virus intra-host genetic diversity during human-to-mosquito transmission.dengue virus (denv) infection of an individual human or mosquito host produces a dynamic population of closely-related sequences. this intra-host genetic diversity is thought to offer an advantage for arboviruses to adapt as they cycle between two very different host species, but it remains poorly characterized. to track changes in viral intra-host genetic diversity during horizontal transmission, we infected aedes aegypti mosquitoes by allowing them to feed on denv2-infected patients. we then p ...201526325059
pharmacological and genetic evidence for gap junctions as potential new insecticide targets in the yellow fever mosquito, aedes aegypti.the yellow fever mosquito aedes aegypti is an important vector of viral diseases that impact global health. insecticides are typically used to manage mosquito populations, but the evolution of insecticide resistance is limiting their effectiveness. thus, identifying new molecular and physiological targets in mosquitoes is needed to facilitate insecticide discovery and development. here we test the hypothesis that gap junctions are valid molecular and physiological targets for new insecticides. g ...201526325403
detection of dengue virus in mosquito extracts and human clinical samples using a field expedient molecular platform.dengue fever occurs in localized outbreaks and can significantly erode troop strength and mission readiness. timely identification of dengue virus (denv) provides for rapid and appropriate patient management decisions, such as medical evacuation and supportive therapies, as well as help to promote force health protection through vector control and personal protective measures. the "ruggedized" advanced pathogen identification device is a field-friendly pcr (polymerase chain reaction) platform th ...201526327544
analysis of dengue virus genetic diversity during human and mosquito infection reveals genetic constraints.dengue viruses (denv) cause debilitating and potentially life-threatening acute disease throughout the tropical world. while drug development efforts are underway, there are concerns that resistant strains will emerge rapidly. indeed, antiviral drugs that target even conserved regions in other rna viruses lose efficacy over time as the virus mutates. here, we sought to determine if there are regions in the denv genome that are not only evolutionarily conserved but genetically constrained in thei ...201526327586
nanoparticles as potential new generation broad spectrum antimicrobial agents.the rapid emergence of antimicrobial resistant strains to conventional antimicrobial agents has complicated and prolonged infection treatment and increased mortality risk globally. furthermore, some of the conventional antimicrobial agents are unable to cross certain cell membranes thus, restricting treatment of intracellular pathogens. therefore, the disease-causing-organisms tend to persist in these cells. however, the emergence of nanoparticle (np) technology has come with the promising broad ...201526329777
evidence for the expression of abundant micrornas in the locust genome.substantial accumulation of neutral sequences accounts for genome size expansion in animal genomes. numerous novel micrornas (mirnas), which evolve in a birth and death manner, are considered evolutionary neutral sequences. the migratory locust is an ideal model to determine whether large genomes contain abundant neutral mirnas because of its large genome size. a total of 833 mirnas were discovered, and several mirnas were randomly chosen for validation by northern blot and rip-qpcr. three addit ...201526329925
efficacy of tolypocladium cylindrosporum against aedes aegypti eggs, larvae and adults.the aim of this study was to test the activity of seven tolypocladium cylindrosporum isolates against different developmental stages of aedes aegypti.201526332164
the novel abc transporter abch1 is a potential target for rnai-based insect pest control and resistance management.insect pests cause serious crop damage and develop high-level resistance to chemical insecticides and bacillus thuringiensis (bt) insecticidal cry toxins. a new promising approach for controlling them and overcoming this resistance is rna interference (rnai). the rnai-based insect control strategy depends on the selection of suitable target genes. in this study, we cloned and characterized a novel abc transporter gene pxabch1 in diamondback moth, plutella xylostella (l.). phylogenetic analysis s ...201526333918
crepuscular behavioral variation and profiling of opsin genes in anopheles gambiae and anopheles stephensi (diptera: culicidae).we understand little about photo-preference and the molecular mechanisms governing vision-dependent behavior in vector mosquitoes. investigations of the influence of photo-preference on adult mosquito behaviors such as endophagy and exophagy and endophily and exophily will enhance our ability to develop and deploy vector-targeted interventions and monitoring techniques. our laboratory-based analyses have revealed that crepuscular period photo-preference differs between an. gambiae and an. stephe ...201526334802
real-time pcr tests in dutch exotic mosquito surveys; implementation of aedes aegypti and aedes albopictus identification tests, and the development of tests for the identification of aedes atropalpus and aedes japonicus japonicus (diptera: culicidae).since 2009, the netherlands food and consumer product safety authority carries out surveys focusing on, amongst others, the presence of invasive mosquito species (ims). special attention is given to exotic container-breeding aedes species aedes aegypti (l.), aedes albopictus (skuse), aedes atropalpus (coquillett), and aedes japonicus japonicus (theobald). this study describes the implementation of real-time pcr tests described by hill et al. (2008) for the identification of ae. aegypti and ae. a ...201526334807
evaluation of simultaneous transmission of chikungunya virus and dengue virus type 2 in infected aedes aegypti and aedes albopictus (diptera: culicidae).the simultaneous transmission of chikungunya virus (chikv) and dengue viruses (denv) has been a major public health concern because of their sympatric distribution and shared mosquito vectors. groups of aedes aegypti (l.) and aedes albopictus (skuse) were orally infected with 1.5 × 10(5) pfu/ml of chikv and 3.2 × 10(6) ffu/ml of denv-2 simultaneously or separately in inverse orders and evaluated for dissemination and transmission by qrt-pcr. simultaneous dissemination of both viruses was detecte ...201526334820
large scale rnai screen in tribolium reveals novel target genes for pest control and the proteasome as prime target.insect pest control is challenged by insecticide resistance and negative impact on ecology and health. one promising pest specific alternative is the generation of transgenic plants, which express double stranded rnas targeting essential genes of a pest species. upon feeding, the dsrna induces gene silencing in the pest resulting in its death. however, the identification of efficient rnai target genes remains a major challenge as genomic tools and breeding capacity is limited in most pest insect ...201526334912
seasonal genetic changes of aedes aegypti (diptera: culicidae) populations in selected sites of cebu city, philippines.aedes aegypti (l.) is the primary vector of dengue virus in the philippines, where dengue is endemic. we examined the genetic changes of ae. aegypti collected from three selected sites in cebu city, philippines, during the relatively wet (2011-2012) and dry seasons (2012 and 2013). a total of 493 ae. aegypti adults, reared in the laboratory from field-collected larvae, were analyzed using 11 microsatellite loci. seasonal variation was observed in allele frequencies and allelic richness. average ...201526335470
evaluating liquid and granular bacillus thuringiensis var. israelensis broadcast applications for controlling vectors of dengue and chikungunya viruses in artificial containers and tree holes.the principal vectors of chikungunya and dengue viruses typically oviposit in water-filled artificial and natural containers, including tree holes. despite the risk these and similar tree hole-inhabiting mosquitoes present to global public health, surprisingly few studies have been conducted to determine an efficient method of applying larvicides specifically to tree holes. the stihl sr 450, a backpack sprayer commonly utilized during military and civilian vector control operations, may be suita ...201526335473
venom allergen 5 is associated with deltamethrin resistance in culex pipiens pallens (diptera: culicidae).the mosquito, culex pipiens pallens (l.), is an important vector of encephalitis and filariasis in northern china. the control of these mosquitoes occurs primarily via the use of pyrethroid insecticides, such as deltamethrin. the widespread and improper application of pyrethroid has resulted in the evolution of pyrethroid resistance amongst many mosquito populations, including cx. pipiens pallens. previous studies using high-throughput transcriptome sequencing have identified that the venom alle ...201526335474
introduction and establishment of aedes albopictus (diptera: culicidae) in managua, nicaragua.aedes aegypti (l.) is the main vector of dengue virus and more recently chikungunya virus in latin america. however, the asian tiger mosquito aedes albopictus (skuse, 1894) is expanding its global range and increasing its role in transmission of these diseases. in this report, we suggest that ae. albopictus was introduced to the department of managua, nicaragua, in 2010 via two independent routes and demonstrate its dissemination and establishment in urban neighborhoods by 2012. the coexistence ...201526335479
evidence for aedes aegypti (diptera: culicidae) oviposition on boats in the peruvian amazon.dengue vector aedes aegypti l. is invading peri-urban and rural areas throughout latin america. our previous research in the peruvian amazon has shown that river boats are heavily infested with immature and adult ae. aegypti mosquitoes, likely playing a major role in their long-distance dispersal and successful invasion. however, the presence of immature mosquitoes provides no information about the timing of oviposition, and whether it took place in the boats. here, we used baited ovitraps deplo ...201526335482
aedes aegypti (diptera: culicidae) in mauritania: first report on the presence of the arbovirus mosquito vector in nouakchott.aedes aegypti l. (diptera: culicidae) is a major vector of yellow fever, dengue, and chikungunya viruses throughout tropical and subtropical areas of the world. although the southernmost part of mauritania along the senegal river has long been recognized at risk of yellow fever transmission, aedes spp. mosquitoes had never been reported northwards in mauritania. here, we report the first observation of aedes aegypti aegypti (l.) and aedes (ochlerotatus) caspius (pallas, 1771) in the capital city ...201526335483
ross river virus: many vectors and unusual hosts make for an unpredictable pathogen. 201526335937
potential effects of climate change on ecological interaction outcomes between two disease-vector mosquitoes: a mesocosm experimental study.the objective of this study was to experimentally assess the effects of different climate change scenarios on the outcomes of interactions between aedes aegypti (l.) and culex quinquefasciatus (say) (diptera: culicidae) larvae. the experimental design maintained a constant density of specimens while the proportion of the species in different experimental climate change scenarios varied. our results indicate that survival of the two species was not affected, but larval development and pupation ti ...201526336208
larvicidal and biting deterrent activity of essential oils of curcuma longa, ar-turmerone, and curcuminoids against aedes aegypti and anopheles quadrimaculatus (culicidae: diptera).essential oils and extract of curcuma longa, ar-turmerone, and curcuminoids were evaluated for their larvicidal and deterrent activity against mosquitoes. ar-turmerone and curcuminoids constituted 36.9, 24.9 and 50.6% of rhizome oil, leaf oil, and rhizome extract, respectively. ar-turmerone was the major compound of the rhizome oil (36.9%) and leaf oil (24.9%). the ethanolic extract had 15.4% ar-turmerone with 6.6% bisdesmethoxycurcumin, 6.1% desmethoxycurcumin, and 22.6% curcumin. in in vitro s ...201526336212
late-instar behavior of aedes aegypti (diptera: culicidae) larvae in different thermal and nutritive environments.the effects of temperature on ectotherm growth have been well documented. how temperature affects foraging behavior is less well explored, and has not been studied in larval mosquitoes. we hypothesized that temperature changes foraging behavior in the aquatic larval phase of the mosquito, aedes aegypti l. based on empirical results in other systems, we predicted that foraging effort would increase at higher temperatures in these insects. we tested this prediction over three temperature condition ...201526336228
comparison of the insecticidal characteristics of commercially available plant essential oils against aedes aegypti and anopheles gambiae (diptera: culicidae).aedes aegypti and anopheles gambiae are two mosquito species that represent significant threats to global public health as vectors of dengue virus and malaria parasites, respectively. although mosquito populations have been effectively controlled through the use of synthetic insecticides, the emergence of widespread insecticide-resistance in wild mosquito populations is a strong motivation to explore new insecticidal chemistries. for these studies, ae. aegypti and an. gambiae were treated with c ...201526336230
sterol carrier protein 2, a critical host factor for dengue virus infection, alters the cholesterol distribution in mosquito aag2 cells.host factors that enable dengue virus (denv) to propagate in the mosquito host cells are unclear. it is known that cellular cholesterol plays an important role in the life cycle of denv in human host cells but unknown if the lipid requirements differ for mosquito versus mammalian. in mosquito aedes aegypti, sterol carrier protein 2 (scp-2) is critical for cellular cholesterol homeostasis. in this study, we identified scp-2 as a critical host factor for denv production in mosquito aag2 cells. tre ...201526336241
cold season mortality under natural conditions and subsequent hatching response of aedes (stegomyia) aegypti (diptera: culicidae) eggs in a subtropical city of argentina.in temperate and subtropical regions, populations of aedes (stegomyia) aegypti (l.) survive unfavorable winter conditions in the egg stage. knowing their survival rates can be of great interest for the health authorities in charge of control activities. in this study, we analyzed the mortality of ae. aegypti eggs exposed to the cold season as well as their hatching patterns under laboratory conditions in the city of resistencia, chaco, argentina. the mortality rate was 48.6%. no statistically si ...201526336247
simulated seasonal photoperiods and fluctuating temperatures have limited effects on blood feeding and life history in aedes triseriatus (diptera: culicidae).biotic and abiotic factors change seasonally and impact life history in temperate-zone ectotherms. temperature and photoperiod are factors that change in predictable ways. most studies testing for effects of temperature on vectors use constant temperatures and ignore potential correlated effects of photoperiod. in two experiments, we tested for effects of larval rearing environments creating ecologically relevant temperatures and photoperiods simulating early and late season conditions (june and ...201526336255
essential oils of echinophora lamondiana (apiales: umbelliferae): a relationship between chemical profile and biting deterrence and larvicidal activity against mosquitoes (diptera: culicidae).the essential oils from the flower, leaf, and stem of echinophora lamondiana b.yildiz et z.bahcecioglu were analyzed by gas chromatography-flame ionization detection and gas chromatography-mass spectrometry. in total, 41, 37, and 44 compounds were identified, which accounted for 98.0, 99.1, and 97.0% of flower, leaf, and stem essential oils, respectively. the monoterpenic hydrocarbons were found to be high in all samples of the essential oils. the major components of essential oils from flower, ...201526336285
interspecific larval competition differentially impacts adult survival in dengue vectors.mosquitoes often experience intraspecific and interspecific competition among larvae attributable to high densities and nutrient limitation, especially container mosquitoes including aedes aegypti (l.) and aedes albopictus (skuse). density-dependent effects on larvae impact adult production and adult traits that influence transmission of arboviruses. to improve our understanding of the mechanisms by which density-dependence influences transmission and identify species-specific traits, we tested ...201526336301
evaluation of insect growth regulators against field-collected aedes aegypti and aedes albopictus (diptera: culicidae) from malaysia.susceptibility status of aedes aegypti (l.) and aedes albopictus skuse larvae obtained from 12 states in malaysia were evaluated against five insect growth regulators (igrs), namely, pyriproxyfen, methoprene, diflubenzuron, cyromazine, and novaluron under laboratory conditions. field populations of ae. aegypti exhibited moderate resistance toward methoprene and low resistance toward pyriproxyfen, with resistance ratios of 12.7 and 1.4, respectively, but susceptibility to diflubenzuron, cyromazin ...201526336304
efficacy of essential oil from cananga odorata (lamk.) hook.f. & thomson (annonaceae) against three mosquito species aedes aegypti (l.), anopheles dirus (peyton and harrison), and culex quinquefasciatus (say).the essential oil of cananga odorata flowers was evaluated for oviposition-deterrent, ovicidal, insecticidal, and repellent activities toward three mosquito species: aedes aegypti, anopheles dirus, and culex quinquefasciatus. oviposition deterrence of the oil was evaluated on gravid females using oviposition deterrence bioassay. the results showed that 10 % ca. odorata exhibited high percent effective repellency against oviposition at 99.4 % to ae. aegypti, 97.1 % to an. dirus, and 100 % to cx. ...201526337270
impact of insect salivary proteins in blood feeding, host immunity, disease, and in the development of biomarkers for vector exposure.functional genomic approaches based on expression of recombinant proteins linked to biochemical and disease model approaches resulted in the discovery of novel biological activities and the role some of these proteins play in disease transmission. importantly, the expression of salivary proteins was recently shown to be affected by environmental factors and by the presence of the pathogen in the salivary gland. a practical application resulting from insect saliva research is the use of insect an ...026339571
meta-analysis of studies on chemical, physical and biological agents in the control of aedes aegypti.aedes aegypti is a vector of international concern because it can transmit to humans three important arboviral diseases: yellow fever, dengue and chikungunya. epidemics that are repeated year after year in a variety of urban centers indicate that there are control failures, allowing the vector to continue expanding.201526341708
identification and characterization of novel membrane-bound prl protein tyrosine phosphatases from setaria cervi, a bovine filarial parasite.a significant amount of protein tyrosine phosphatase (ptp) activity was detected in the detergent-soluble membrane-bound fraction of setaria cervi, a bovine filarial parasite. the membrane-bound ptp activity was significantly inhibited when the adult parasites were exposed to compounds having antifilarial activity like aspirin and sk7 as well as phenylarsine oxide, a specific ptp inhibitor suggesting that this activity is stress regulated. further, this enzyme was purified as a single protein of ...201526341797
development and validation of an elisa kit (yf mac-hd) to detect igm to yellow fever virus.yellow fever virus (yfv) is endemic in tropical and sub-tropical regions of the world, with around 180,000 human infections a year occurring in africa. serologic testing is the chief laboratory diagnostic means of identifying an outbreak and to inform the decision to commence a vaccination campaign. the world health organization disseminates the reagents for yfv testing to african reference laboratories, and the us centers for disease control and prevention (cdc) is charged with producing and pr ...201526342907
studies on insecticide susceptibility of aedes aegypti (linn) and aedes albopictus (skuse) vectors of dengue and chikungunya in andaman and nicobar islands, india.dengue and chikungunya are important arboviral infections in the andaman islands. competent vectors viz. aedes aegypti and aedes albopictus are widely prevalent. the most effective proven method for interrupting the transmission of these arboviruses is vector control, mediated through insecticides. currently, ddt and temephos are the insecticides used for vector control in these islands. lack of information on susceptibility necessitated assessing the susceptibility profile of a. aegypti and a. ...201526344869
mosquitocidal properties of bacillus species isolated from mangroves of vellar estuary, southeast coast of india.samples collected from the mangroves of vellar estuary yielded a mosquitocidal bacterium, whose secondary metabolites exhibited mosquito larvicidal and pupicidal activity. the bacterium was isolated using standard microbiological methods and identified using classical biochemical tests. the mosquitocidal bacterium was identified as bacillus subtilis, bacillus thuringiensis, bacillus sphaericus and bacillus cereus. mosquitocidal metabolite(s) was separated from the culture supernatant of the bact ...201326345039
comparison of methods for xenomonitoring in vectors of lymphatic filariasis in northeastern tanzania.monitoring wuchereria bancrofti infection in mosquitoes (xenomonitoring) can play an important role in determining when lymphatic filariasis has been eliminated, or in focusing control efforts. as mosquito infection rates can be low, a method for collecting large numbers of mosquitoes is necessary. gravid traps collected large numbers of culex quinquefasciatus in tanzania, and a collection method that targets mosquitoes that have already fed could result in increased sensitivity in detecting w. ...201526350454
impact of human mobility on the emergence of dengue epidemics in pakistan.the recent emergence of dengue viruses into new susceptible human populations throughout asia and the middle east, driven in part by human travel on both local and global scales, represents a significant global health risk, particularly in areas with changing climatic suitability for the mosquito vector. in pakistan, dengue has been endemic for decades in the southern port city of karachi, but large epidemics in the northeast have emerged only since 2011. pakistan is therefore representative of ...201526351662
evolutionary history of the photolyase/cryptochrome superfamily in eukaryotes.photolyases and cryptochromes are evolutionarily related flavoproteins, which however perform distinct physiological functions. photolyases (phr) are evolutionarily ancient enzymes. they are activated by light and repair dna damage caused by uv radiation. although cryptochromes share structural similarity with dna photolyases, they lack dna repair activity. cryptochrome (cry) is one of the key elements of the circadian system in animals. in plants, cry acts as a blue light receptor to entrain ci ...201526352435
behavioral response of aedes aegypti (diptera: culicidae) larvae to synthetic and natural attractants and repellents.aedes aegypti (l.) (diptera: culicidae) is the key vector of three important arboviral diseases: dengue, yellow fever, and chikungunya. immature stages of this species inhabit human-made containers placed in residential landscapes. in this study, we evaluated a few compounds in a sensitive behavioral assay with ae. aegypti larvae. the orientation of larvae to different compounds was surveyed using a performance index (pi). the pi represents the response to each odorant, where a value of +1 is in ...201526352935
detection of wolbachia from field collected aedes albopictus skuse in malaysia.wolbachia-based vector control strategies have been proposed as a mean to augment the existing measures for controlling dengue vector. prior to utilizing wolbachia in novel vector control strategies, it is crucial to understand the wolbachia-mosquito interactions. many studies have only focused on the prevalence of wolbachia in female aedes albopictus with lack of attention on wolbachia infection on the male ae. albopictus which also affects the effective expression of wolbachia induced- cytopla ...026354218
ecuador paraiso escondido virus, a new flavivirus isolated from new world sand flies in ecuador, is the first representative of a novel clade in the genus flavivirus.a new flavivirus, ecuador paraiso escondido virus (epev), named after the village where it was discovered, was isolated from sand flies (psathyromyia abonnenci, formerly lutzomyia abonnenci) that are unique to the new world. this represents the first sand fly-borne flavivirus identified in the new world. epev exhibited a typical flavivirus genome organization. nevertheless, the maximum pairwise amino acid sequence identity with currently recognized flaviviruses was 52.8%. phylogenetic analysis o ...201526355096
improving the effectiveness of three essential oils against aedes aegypti (linn.) and anopheles dirus (peyton and harrison).repellency of essential oil extracted from curcuma longa, eucalyptus globulus, and citrus aurantium at various concentrations (5, 10, 15, 20, and 25 %) with and without 5 % vanillin was evaluated against female mosquitoes: aedes aegypti and anopheles dirus. the comparisons were made with a commercial chemical repellent (n,n-diethyl-3-methylbenzamide (deet) 25 % w/w; kor yor 15) by arm in cage method. it was found that the essential oils with 5 % vanillin gave the longest lasting period against t ...201626358103
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