Publications

systemic inflammation, innate immunity and pathogenesis after zika virus infection in cynomolgus macaques are modulated by strain-specificity within the asian lineage.	zika virus (zikv) is an emerging arbovirus with recent global expansion. historically, zikv infections with asian lineages have been associated with mild disease such as rash and fever. however, recent asian sub-lineages have caused outbreaks in the south pacific and latin america with increased prevalence of neurological disorders in infants and adults. asian sub-lineage differences may partially explain the range of disease severity observed. however, the effect of asian sub-lineage difference ...	2021	34120576
the utilization of advance telemetry to investigate critical physiological parameters including electroencephalography in cynomolgus macaques following aerosol challenge with eastern equine encephalitis virus.	most alphaviruses are mosquito-borne and can cause severe disease in humans and domesticated animals. in north america, eastern equine encephalitis virus (eeev) is an important human pathogen with case fatality rates of 30-90%. currently, there are no therapeutics or vaccines to treat and/or prevent human infection. one critical impediment in countermeasure development is the lack of insight into clinically relevant parameters in a susceptible animal model. this study examined the disease course ...	2021	34138849
cell-mimicking nanodecoys neutralize sars-cov-2 and mitigate lung injury in a non-human primate model of covid-19.	coronavirus disease 2019 (covid-19), caused by severe acute respiratory syndrome coronavirus 2 (sars-cov-2), has grown into a global pandemic, and only a few antiviral treatments have been approved to date. angiotensin-converting enzyme 2 (ace2) plays a fundamental role in sars-cov-2 pathogenesis because it allows viral entry into host cells. here we show that ace2 nanodecoys derived from human lung spheroid cells (lscs) can bind and neutralize sars-cov-2 and protect the host lung cells from inf ...	2021	34140674
pre-existing simian immunodeficiency virus infection increases expression of t cell markers associated with activation during early mycobacterium tuberculosis coinfection and impairs tnf responses in granulomas.	tuberculosis (tb) is the leading infectious cause of death among people living with hiv. people living with hiv are more susceptible to contracting mycobacterium tuberculosis and often have worsened tb disease. understanding the immunologic defects caused by hiv and the consequences it has on m. tuberculosis coinfection is critical in combating this global health epidemic. we previously showed in a model of siv and m. tuberculosis coinfection in mauritian cynomolgus macaques (mcm) that siv/m. tu ...	2021	34145063
sars-cov-2 aerosol exhaled by experimentally infected cynomolgus monkeys.	we analyzed size of severe acute respiratory coronavirus 2 (sars-cov-2) aerosol particles shed by experimentally infected cynomolgus monkeys. most exhaled particles were small, and virus was mainly released early during infection. by postinfection day 6, no virus was detected in breath, but air in the isolator contained large quantities of aerosolized virus.	2021	34152969
natural history of disease in cynomolgus monkeys exposed to ebola virus kikwit strain demonstrates the reliability of this non-human primate model for ebola virus disease.	filoviruses (family filoviridae genera ebolavirus and marburgvirus) are negative-stranded rna viruses that cause severe health effects in humans and non-human primates, including death. except in outbreak settings, vaccines and other medical countermeasures against ebola virus (ebov) will require testing under the fda animal rule. multiple vaccine candidates have been evaluated using cynomolgus monkeys (cm) exposed to ebov kikwit strain. to the best of our knowledge, however, animal model develo ...	2021	34214118
pharmacokinetic profiles of a sars-cov-2 neutralizing antibody bd-604 in cynomolgus monkeys.	currently, severe acute respiratory syndrome coronavirus 2 (sars-cov-2) has spread worldwide as a severe pandemic and effective therapeutic medications are urgently needed. as reported previously, bd-604 is a fully human monoclonal antibody with strong in vitro and in vivo neutralizing activity to sars-cov-2.	2021	34215019
generation of a bactrian camel hepatitis e virus by a reverse genetics system.	bactrian camel hepatitis e virus (hev) is a novel hev belonging to genotype 8 (hev-8) in the orthohepevirus a species of the genus hepevirus in the family hepeviridae. hev-8 cross-transmits to cynomolgus monkeys and has a potential risk for zoonotic infection. until now, neither a cell-culture system to grow the virus nor a reverse genetics system to generate the virus has been developed. to generate replication-competent hev-8 and to establish a cell-culture system, we synthesized capped genomi ...	2021	34242156
in vitro characterization of fitness and convalescent antibody neutralization of sars-cov-2 cluster 5 variant emerging in mink at danish farms.	in addition to humans, severe acute respiratory syndrome coronavirus 2 (sars-cov-2) can transmit to animals that include hamsters, cats, dogs, mink, ferrets, tigers, lions, cynomolgus macaques, rhesus macaques, and treeshrew. among these, mink are particularly susceptible. indeed, 10 countries in europe and north america reported sars-cov-2 infection among mink on fur farms. in denmark, sars-cov-2 spread rapidly among mink farms and spilled-over back into humans, acquiring mutations/deletions wi ...	2021	34248922
markers of viral hepatitis e (hepeviridae, orthohepevirus, orthohepevirus a) in the imported old world monkeys.	viral hepatitis e is a zooanthroponotic disease that occurs in humans and various animals, including monkeys. it is caused by hepatitis e virus (hev) (hepeviridae, orthohepevirus: orthohepevirus a), for which 8 genotypes have been described to date. among them, strains of genotypes 1 and 2 have been isolated from humans, strains of genotypes 3 and 4 from humans and animals, and strains of genotypes 5-8 from animals only. the main threat of the disease is associated with the documented zoonotic t ...	2021	34251155
a modular protein subunit vaccine candidate produced in yeast confers protection against sars-cov-2 in non-human primates.	vaccines against sars-cov-2 have been distributed at massive scale in developed countries, and have been effective at preventing covid-19. access to vaccines is limited, however, in low- and middle-income countries (lmics) due to insufficient supply, high costs, and cold storage requirements. new vaccines that can be produced in existing manufacturing facilities in lmics, can be manufactured at low cost, and use widely available, proven, safe adjuvants like alum, would improve global immunity ag ...	2021	34282417
a recombinant spike protein subunit vaccine confers protective immunity against sars-cov-2 infection and transmission in hamsters.	multiple safe and effective vaccines that elicit immune responses against severe acute respiratory syndrome coronavirus 2 (sars-cov-2) are necessary to respond to the ongoing coronavirus disease 2019 (covid-19) pandemic. here, we developed a protein subunit vaccine composed of spike ectodomain protein (strifk) plus a nitrogen bisphosphonate-modified zinc-aluminum hybrid adjuvant (fh002c). strifk-fh002c generated substantially higher neutralizing antibody titers in mice, hamsters, and cynomolgus ...	2021	34285130
host- and species-dependent quasispecies divergence of severe acute respiratory syndrome coronavirus-2 in non-human primate models.	recently, newly emerging variants of severe acute respiratory syndrome coronavirus 2 (sars-cov-2) have been continuously reported worldwide. however, the precise evaluation of sars-cov-2 microevolution in host is very limited because the exact genetic information of infected virus could not be acquired in human researches. in this report, we performed deep sequencing for seed virus and sars-cov-2 isolated in eight cynomolgus and rhesus macaques at 3 days postinoculation and evaluated single-nucl ...	2021	34305860