Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| effector t-cell differentiation during viral and bacterial infections: role of direct il-12 signals for cell fate decision of cd8(+) t cells. | to study the role of il-12 as a third signal for t-cell activation and differentiation in vivo, direct il-12 signaling to cd8(+) t cells was analyzed in bacterial and viral infections using the p14 t-cell adoptive transfer model with cd8(+) t cells that lack the il-12 receptor. results indicate that cd8(+) t cells deficient in il-12 signaling were impaired in clonal expansion after listeria monocytogenes infection but not after infection with lymphocytic choriomeningitis virus, vaccinia virus or ... | 2009 | 19548244 |
| infections in patients with aplastic anemia. | infection is a major cause of death in patients with aplastic anemia (aa). there are differences between the immunocompromised state of a patient with aa and the patient who is neutropenic due to chemotherapy and this leads to a difference in the infections that they incur. prolonged neutropenia is one of the largest risk factors for the development of infections with the invasive mycoses and bacteria. recovery from neutropenia is directly related to survival, and supportive care plays a large r ... | 2009 | 19549579 |
| particles from wood smoke and road traffic differently affect the innate immune system of the lung. | the effect of particles from road traffic and wood smoke on the innate immune response in the lung was studied in a lung challenge model with the intracellular bacterium listeria monocytogenes. female balb/ca mice were instilled intratracheally with wood smoke particles, particles from road traffic collected during winter (studded tires used; st+), and during autumn (no studded tires; st-), or diesel exhaust particles (dep). simultaneously with, and 1 or 7 days after particle instillation, 10(5) ... | 2009 | 19552530 |
| characterization of probiotic carnobacteria isolated from rainbow trout (oncorhynchus mykiss) intestine. | to characterize two probiotic carnobacterial isolates, carnobacterium maltaromaticum (b26) and c. divergens (b33), derived from rainbow trout (oncorhynchus mykiss) intestine. | 2008 | 19552776 |
| myd88 and type i interferon receptor-mediated chemokine induction and monocyte recruitment during listeria monocytogenes infection. | monocytes play a central role in defense against infection, but the mechanisms promoting monocyte recruitment and activation remain incompletely defined. defense against listeria monocytogenes, an intracellular bacterial pathogen, requires in vivo mcp-1 induction and ccr2-dependent recruitment of ly6c(high) monocytes from bone marrow to sites of infection. herein, we demonstrate that infection of bone marrow-derived macrophages with virulent l. monocytogenes induces mcp-1 expression in two phase ... | 2009 | 19553532 |
| listeria monocytogenes l-forms respond to cell wall deficiency by modifying gene expression and the mode of division. | cell wall-deficient bacteria referred to as l-forms have lost the ability to maintain or build a rigid peptidoglycan envelope. we have generated stable, non-reverting l-form variants of the gram-positive pathogen listeria monocytogenes, and studied the cellular and molecular changes associated with this transition. stable l-form cells can occur as small protoplast-like vesicles and as multinucleated, large bodies. they have lost the thick, multilayered murein sacculus and are surrounded by a cyt ... | 2009 | 19555455 |
| decoupling internalization, acidification and phagosomal-endosomal/lysosomal fusion during phagocytosis of inla coated beads in epithelial cells. | phagocytosis has been extensively examined in 'professional' phagocytic cells using ph sensitive dyes. however, in many of the previous studies, a separation between the end of internalization, beginning of acidification and completion of phagosomal-endosomal/lysosomal fusion was not clearly established. in addition, very little work has been done to systematically examine phagosomal maturation in 'non-professional' phagocytic cells. therefore, in this study, we developed a simple method to meas ... | 2009 | 19557127 |
| inos-producing inflammatory dendritic cells constitute the major infected cell type during the chronic leishmania major infection phase of c57bl/6 resistant mice. | leishmania major parasites reside and multiply in late endosomal compartments of host phagocytic cells. immune control of leishmania growth absolutely requires expression of inducible nitric oxide synthase (inos/nos2) and subsequent production of no. here, we show that cd11b+ cd11c+ ly-6c+ mhc-ii+ cells are the main inos-producing cells in the footpad lesion and in the draining lymph node of leishmania major-infected c57bl/6 mice. these cells are phenotypically similar to inos-producing inflamma ... | 2009 | 19557162 |
| identification of a novel two-peptide lantibiotic, lichenicidin, following rational genome mining for lanm proteins. | lantibiotics are ribosomally synthesized peptide antimicrobials which contain considerable posttranslational modifications. given their usually broad host range and their highly stable structures, there have been renewed attempts to identify and characterize novel members of the lantibiotic family in recent years. the increasing availability of bacterial genome sequences means that in addition to traditional microbiological approaches, in silico screening strategies may now be employed to the sa ... | 2009 | 19561184 |
| antioxidant and antilisterial effect of seed essential oil and organic extracts from zizyphus jujuba. | hydrodistilled volatile oil from the seeds of zizyphus jujuba was analyzed by gc-ms. twenty three compounds representing 91.59% of the total oil was identified. the oil and organic extracts revealed a great potential of antilisterial effect against all five strains of listeria monocytogenes atcc 19111, 19116, 19118, 19166 and 15313. also the oil had strong detrimental effect on the viable count of the tested bacteria. the samples were also subjected to screening for the antioxidant activity by u ... | 2009 | 19563858 |
| cellular accumulation and pharmacodynamic evaluation of the intracellular activity of cem-101, a novel fluoroketolide, against staphylococcus aureus, listeria monocytogenes, and legionella pneumophila in human thp-1 macrophages. | cem-101 is a novel fluoroketolide with lower mics than those of telithromycin and macrolides. our aim was to assess the cellular accumulation and intracellular activity of cem-101 using models developed for analyzing the pharmacokinetics and pharmacological properties of antibiotics against phagocytized bacteria. we used thp-1 macrophages and staphylococcus aureus (atcc 25923 [methicillin (meticillin) sensitive]), listeria monocytogenes (strain egd), and legionella pneumophila (atcc 33153). cem- ... | 2009 | 19564365 |
| blockade of cd137 signaling counteracts polymicrobial sepsis induced by cecal ligation and puncture. | sepsis, a leading cause of death worldwide, involves proinflammatory responses and inefficient bacterial clearance. previously, we have shown that cd137 (4-1bb), a member of the tumor necrosis factor receptor superfamily, plays critical roles in eradicating infective listeria monocytogenes, a gram-positive bacterium, and that stimulation of cd137 protects mice from sepsis-induced death. in this study, we unexpectedly found that cd137 activation aggravated polymicrobial sepsis due to mixed gram-p ... | 2009 | 19564374 |
| staphylococcal alpha-toxin is not sufficient to mediate escape from phagolysosomes in upper-airway epithelial cells. | intracellular staphylococcus aureus has been implicated in the establishment of chronic infections. it is therefore imperative to understand by what means s. aureus is able to survive within cells. here we use two expression systems with a fluorescent readout to assay alpha-toxin expression and function within phagolysosomes of infected upper-airway epithelial cells: avirulent staphylococcus carnosus tm300 and phenotypically alpha-toxin-negative s. aureus laboratory strains. data from cfu recove ... | 2009 | 19564384 |
| first results on the detection of listeria monocytogenes in raw milk: comparison of two different analytical methods. | 2009 | 19568945 | |
| listeria monocytogenes membrane trafficking and lifestyle: the exception or the rule? | listeria monocytogenes is an intracellular bacterial pathogen that promotes its internalization within nonprofessional phagocytes by interacting with specific host cell receptors. l. monocytogenes resides transiently in a membrane-bound compartment before escaping into the host cell cytosol where bacterial proliferation takes place. actin-based motility then promotes cell-to-cell pathogen spread. extensive studies on cytoskeleton rearrangements, membrane trafficking, and other events have establ ... | 2009 | 19575658 |
| a novel fas ligand in mollusk abalone: molecular characterization, immune responses and biological activity of the recombinant protein. | fas ligand is a member of the tnf superfamily that plays an important role by inducing apoptosis and homeostasis of immune responses. the gene encoding fas ligand was isolated from a disk abalone (haliotis discus discus) cdna library, denoted as the abfas ligand. it contains an 1832bp transcript with a 945bp open reading frame, encoding 315 amino acids. the abfas ligand showed characteristic transmembrane and tnf family signature domains. the deduced amino acid comparison showed that the abfas l ... | 2009 | 19576285 |
| a correlation between the virulence and the adhesion of listeria monocytogenes to silicon nitride: an atomic force microscopy study. | listeria monocytogenes is a facultative intracellular gram-positive bacterium that is widely distributed in the environment. despite being pathogenic at the species level, l. monocytogenes in fact comprises a diversity of strains from pathogenic ones that can result in disease and/or mortality to others that are relatively avirulent. the main goal of the current study was to answer the question on whether enhanced binding or attachment of l. monocytogenes to inert surfaces bears any relationship ... | 2009 | 19576735 |
| manipulations of metallothionein gene dose accelerate the response to listeria monocytogenes. | metallothioneins (mts) are cysteine-rich proteins that assist in cellular homeostasis and protect against oxidant injury. mts can be induced by heavy metals and inflammatory mediators and function as free radical scavengers, reservoirs for essential heavy metals, and immunomodulators. in light of mts' roles in responses to stress, we evaluated the in vivo effects of mt gene dose on the course of listeria monocytogenes (lm) infection. lm burden was measured in livers and spleens, and flow cytomet ... | 2009 | 19576872 |
| study on the application of an interspecific competition model for the prediction of microflora behaviour during the fermentation process of s. angelo pgi salami. | 2009 | 19578949 | |
| essential oils against foodborne pathogens and spoilage bacteria in minced meat. | the antimicrobial activity of essential oils of oregano, thyme, basil, marjoram, lemongrass, ginger, and clove was investigated in vitro by agar dilution method and minimal inhibitory concentration (mic) determination against gram-positive (staphylococcus aureus and listeria monocytogenes) and gram-negative strains (escherichia coli and salmonella enteritidis). mic(90%) values were tested against bacterial strains inoculated experimentally in irradiated minced meat and against natural microbiota ... | 2009 | 19580445 |
| gene transcription and virulence potential of listeria monocytogenes strains after exposure to acidic and nacl stress. | gene transcription and virulence potential of two strains of listeria monocytogenes, egd-e and 4140, were compared by quantitative real-time polymerase chain reaction and in a caco-2 in vitro model after exposure to acidic (ph 5.5) and nacl (4.5% w/v) stress. strain-dependent differences in gene transcription were observed both after exposure to shock (six genes) and after long-term adaptation to stress (18 genes). in the shock experiments, a transient induction of clpc and clpe was seen for bot ... | 2009 | 19580450 |
| human alpha-defensins inhibit hemolysis mediated by cholesterol-dependent cytolysins. | many pathogenic gram-positive bacteria release exotoxins that belong to the family of cholesterol-dependent cytolysins. here, we report that human alpha-defensins hnp-1 to hnp-3 acted in a concentration-dependent manner to protect human red blood cells from the lytic effects of three of these exotoxins: anthrolysin o (alo), listeriolysin o, and pneumolysin. hd-5 was very effective against listeriolysin o but less effective against the other toxins. human alpha-defensins hnp-4 and hd-6 and human ... | 2009 | 19581399 |
| some listeria monocytogenes outbreak strains demonstrate significantly reduced invasion, inla transcript levels, and swarming motility in vitro. | listeria monocytogenes can cause a severe invasive food-borne disease known as listeriosis, and large outbreaks of this disease occur occasionally. based on molecular-subtype data, epidemic clone (ec) strains have been defined, including eci and ecia, which have caused listeriosis outbreaks on different continents. while a number of molecular-subtyping studies of outbreak strains have been reported, few comprehensive data sets of virulence-associated characteristics of these strains are availabl ... | 2009 | 19581477 |
| cloning and comparison of phylogenetically related chitinases from listeria monocytogenes egd and enterococcus faecalis v583. | to compare enzymatic activities of two related chitinases, chia and ef0361, encoded by listeria monocytogenes and enterococcus faecalis, respectively. | 2009 | 19583793 |
| antimicrobial mortar surfaces for the improvement of hygienic conditions. | to evaluate the effectiveness of various antimicrobial mortar formulations in inhibiting the growth of a selection of pathogens of environmental and hygienic concern. | 2010 | 19583799 |
| high efficacy of a listeria-based vaccine against metastatic breast cancer reveals a dual mode of action. | most cancer vaccines induce ctl responses to tumor-associated antigens (taa). killing of tumor cells occurs through taa-specific ctl-mediated cytolysis. here, we show that one preventive followed by two therapeutic immunizations with an attenuated listeria monocytogenes (lm)-based vaccine eradicates all metastases and almost the entire primary tumor in the syngeneic, aggressive mouse breast tumor model 4t1. we provide strong evidence that this is due to the combined result of direct kill by list ... | 2009 | 19584282 |
| [headaches and aphasia in an immunocompromised patient]. | we report the case of a 81-year-old, immunocompromised patient, admitted to our hospital with new-onset headaches and word-finding difficulties. the mri of the brain revealed a temporal mass on the left with marginal contrast-enhancement. during the next days listeria monocytogenes grew in the bloodcultures so that the diagnosis of a brain-abscess caused by listeria was established. due to the localisation, surgical drainage of the abscess was not possible, so that a prolonged antibiotic therapy ... | 2009 | 19585445 |
| anti-listerial activity and structure-activity relationships of the six major tyrocidines, cyclic decapeptides from bacillus aneurinolyticus. | six major tyrocidines, purified from the antibiotic tyrothricin complex produced by bacillus aneurinolyticus, showed significant lytic and growth inhibitory activity towards the gram+ bacteria, micrococcus luteus and listeria monocytogenes, but not against the gram- bacterium, escherichia coli. the isolated natural tyrocidines were in particular more active against the leucocin a (antimicrobial peptide) resistant strain, l. monocytogenes b73-mr1, than the sensitive l. monocytogenes b73 strain. r ... | 2009 | 19586775 |
| nisin, rosemary, and ethylenediaminetetraacetic acid affect the growth of listeria monocytogenes on ready-to-eat turkey ham stored at four degrees celsius for sixty-three days. | the objectives of this study were to determine the anti-listeria and general antimicrobial properties of nisin, rosemary, and edta alone and in combination on listeria monocytogenes inoculated on ready-to-eat vacuum-packaged diced turkey ham and to ascertain the effects of the treatments on ph and objective color. the turkey hams were cut into 0.5-cm pieces, inoculated with a l. monocytogenes cocktail containing 5 strains of the bacterium, and treated with either no treatment and no inoculum (ne ... | 2009 | 19590093 |
| seroprevalence of neospora caninum in dairy cattle ranches with high abortion rate: special emphasis to serologic co-existence with toxoplasma gondii, brucella abortus and listeria monocytogenes. | the aim of this study was to determine seroprevalence of neospora caninum in dairy cattle ranches with higher abortion rates and co-existence of neospora caninum, toxoplasma gondii, brucella abortus, and listeria monocytogenes antibodies. the blood samples were collected from dairy cows with history of abortion (n=234) as well as from pregnant cows that were (n=323) kept in the same ranches. n. caninum seroprevalence was 10.77% (60/557). the co-existence rate of n. caninum seropositivity with t. ... | 2009 | 19592171 |
| itch k63-ubiquitinates the nod2 binding protein, rip2, to influence inflammatory signaling pathways. | the inability to coordinate the signaling pathways that lead to proper cytokine responses characterizes the pathogenesis of inflammatory diseases such as crohn's disease. the crohn's disease susceptibility protein, nod2, helps coordinate cytokine responses upon intracellular exposure to bacteria, and this signal coordination by nod2 is accomplished, in part, through k63-linked polyubiquitin chains that create binding surfaces for the scaffolding of signaling complexes. | 2009 | 19592251 |
| an experimental and computational study of the effect of acta polarity on the speed of listeria monocytogenes actin-based motility. | listeria monocytogenes is a pathogenic bacterium that moves within infected cells and spreads directly between cells by harnessing the cell's dendritic actin machinery. this motility is dependent on expression of a single bacterial surface protein, acta, a constitutively active arp2,3 activator, and has been widely studied as a biochemical and biophysical model system for actin-based motility. dendritic actin network dynamics are important for cell processes including eukaryotic cell motility, c ... | 2009 | 19593363 |
| the origin of listeria monocytogenes 4b isolates is signified by subproteomic profiling. | among the 13 known serovars of listeria monocytogenes, strains exhibiting the serovar 4b are the most prevalently involved in epidemics of human listeriosis. the molecular reasons for the major involvement of serovar 4b strains in all major foodborne outbreaks in contrast to the lower prevalence of this serovar among food isolates remain indefinite. in order to provide further insight in the protein expression of l. monocytogenes 4b strains, the cytoplasmic and extracellular proteomes of l. mono ... | 2009 | 19596086 |
| selective inhibition of nicotinamide adenine dinucleotide kinases by dinucleoside disulfide mimics of nicotinamide adenine dinucleotide analogues. | diadenosine disulfide (5) was reported to inhibit nad kinase from listeria monocytogenes and the crystal structure of the enzyme-inhibitor complex has been solved. we have synthesized tiazofurin adenosine disulfide (4) and the disulfide 5, and found that these compounds were moderate inhibitors of human nad kinase (ic(50)=110 microm and ic(50)=87 microm, respectively) and mycobacterium tuberculosis nad kinase (ic(50)=80 microm and ic(50)=45 microm, respectively). we also found that nad mimics wi ... | 2009 | 19596199 |
| selective expansion of the monocytic lineage directed by bacterial infection. | ccr2-mediated recruitment of ly6c(high) monocytes is essential for defense against a range of microbial pathogens. although our understanding of monocyte trafficking to inflammatory sites is increasing, how innate immune inflammation influences monocyte development and maturation during microbial infection remains undefined. herein, we demonstrate that infection with the intracellular bacterial pathogen listeria monocytogenes specifically and selectively promotes monopoiesis. systemic infection ... | 2009 | 19596996 |
| the role of anorexia in resistance and tolerance to infections in drosophila. | most infections induce anorexia but its function, if any, remains unclear. because this response is common among animals, we hypothesized that infection-induced diet restriction might be an adaptive trait that modulates the host's ability to fight infection. two defense strategies protect hosts against infections: resistance, which is the ability to control pathogen levels, and tolerance, which helps the host endure infection-induced pathology. here we show that infected fruit flies become anore ... | 2009 | 19597539 |
| characterization of listeria monocytogenes isolates from human listeriosis cases in italy. | the objective of this study was to characterize by serotyping, pulsed-field gel electrophoresis (pfge), and pcr amplification of virulence genes and markers of epidemic clones i, ii, and iii (eci, ecii, and eciii) 54 human isolates from apparently sporadic cases of infection occurring in the lombardy region and in the province of florence, tuscany, italy, in the years 1996 to 2007. listeria monocytogenes isolates were provided by the clinical microbiology laboratories of the lombardy region and ... | 2009 | 19605584 |
| the magnitude of cd4+ t cell recall responses is controlled by the duration of the secondary stimulus. | the parameters controlling the generation of robust cd4(+) t cell recall responses remain poorly defined. in this study, we compare recall responses by cd4(+) and cd8(+) memory t cells following rechallenge. homologous rechallenge of mice immune to either lymphocytic choriomeningitis virus or listeria monocytogenes results in robust cd8(+) t cell recall responses but poor boosting of cd4(+) t cell recall responses in the same host. in contrast, heterologous rechallenge with a pathogen sharing on ... | 2009 | 19605694 |
| relationship between the physicochemical properties of nonchitinolytic listeria and salmonella and their attachment to shrimp carapace. | listeria and salmonella are important foodborne pathogens normally associated with the shrimp production chain. this study investigated the potential of salmonella typhimurium, salmonella senftenberg, and listeria monocytogenes (scott a and v7) to attach to and colonize shrimp carapace. attachment and colonization of listeria and salmonella were demonstrated. shrimp abdominal carapaces showed higher levels of bacterial attachment (p < 0.05) than did head carapaces. listeria consistently exhibite ... | 2009 | 19610328 |
| inactivation of listeria monocytogenes, escherichia coli o157:h7, and salmonella typhimurium with compounds available in households. | solutions of selected household products were tested for their effectiveness against listeria monocytogenes, escherichia coli o157:h7, and salmonella typhimurium. hydrogen peroxide (1.5 and 3%), vinegar (2.5 and 5% acetic acid), baking soda (11, 33, and 50% sodium bicarbonate), household bleach (0.0314, 0.0933, and 0.670% sodium hypochlorite), 5% acetic acid (prepared from glacial acetic acid), and 5% citric acid solutions were tested against the three pathogens individually (five-strain composi ... | 2009 | 19610330 |
| effects of essential oil treatment, gas atmosphere, and storage temperature on listeria monocytogenes in a model vegetable system. | natural antimicrobials such as plant essential oils (eos) may be useful for controlling pathogenic bacteria on fresh-cut vegetables. the antilisterial properties of eos (thyme, oregano, and rosemary), in combination with different storage atmospheres (air, 5% co2-2% o2-93% n2, and 20% co2-1% o2-79% n2) and temperatures (4 and 80c), were examined using a gas flow-through system combined with a vegetable agar model. the antimicrobial effects of the eos varied depending on the oil, the listeria str ... | 2009 | 19610331 |
| short-term bactericidal efficacy of lauric arginate against listeria monocytogenes present on the surface of frankfurters. | the antimicrobial lauric arginate (lae) alone or in combination with an antimicrobial liquid smoke extract was studied as a postlethality treatment against listeria monocytogenes on vacuum-packaged frankfurters. treatment with 2 ml of 5,000 ppm of lae reduced l. monocytogenes from 7.13 to 5.82 log cfu per package on day 0, and treatment with 3 ml reduced populations from 7.22 to 5.79 log cfu per package on day 0. treatment with lae and smoke flavor did not result in significantly different popul ... | 2009 | 19610332 |
| broad range evaluation of the matrix solubilization (matrix lysis) strategy for direct enumeration of foodborne pathogens by nucleic acids technologies. | a previously published rapid (<5 h) proof-of-concept protocol for the concentration of the foodborne pathogen listeria monocytogenes from milk, based on the solubilization of the food matrix, was further evaluated. the original protocol was modified to detect gram-negative and other gram-positive bacteria and to broaden the range of food matrices by using lutensol instead of sodium dodecyl sulfate as the main detergent in the buffer. a new protocol using a protease and sucrose buffer was establi ... | 2009 | 19610333 |
| simultaneous identification of seven foodborne pathogens and escherichia coli (pathogenic and nonpathogenic) using capillary electrophoresis-based single-strand conformation polymorphism coupled with multiplex pcr. | the objective of this study was to develop a novel technique for parallel analysis of eight important foodborne microbes using capillary electrophoresis-based single-strand conformation polymorphism (ce-sscp) coupled with multiplex pcr. specific primers for multiplex pcr amplification of the 16s rrna gene were designed, corresponding to eight species of bacteria, including escherichia coli, clostridium perfringens, campylobacter jejuni, salmonella enterica, listeria monocytogenes, vibrio parahae ... | 2009 | 19610337 |
| prevalence and genetic diversity of listeria monocytogenes in vacuum-packaged ready-to-eat meat products at retail markets in latvia. | nine groups of different retail ready-to-eat vacuum-packaged meat products from 10 baltic meat processing plants were analyzed for presence and numbers of listeria monocytogenes at the end of shelf life. a total of 38 (18%) of 211 samples tested positive for l. monocytogenes serotype 1/2a (88%) or 1/2c (12%). the prevalence of l. monocytogenes in cold-smoked, sliced, vacuum-packaged beef and pork products (42%) was significantly higher than in cooked, sliced, vacuum-packaged meat products (0.8%) ... | 2009 | 19610341 |
| carnitine enhances the growth of listeria monocytogenes in infant formula at 7 degrees c. | carnitine has beneficial vitamin-like qualities and functions as a compatible solute. the presence of l-carnitine in infant formula significantly promoted the growth of listeria monocytogenes at 7 degrees c, whereas disruption of the genes for carnitine and glycine betaine uptake compromised growth of the l. monocytogenes mutant in formula containing carnitine that was stored at 7 degrees c. thus, addition of carnitine to baby formula may pose a potential food safety risk. | 2009 | 19610343 |
| removal of listeria monocytogenes biofilms from stainless steel by use of ultrasound and ozone. | the objective of this study was to determine the efficacy of power ultrasound and ozonation used individually, and in tandem, for the removal of listeria monocytogenes biofilms from stainless steel chips. stainless steel chips were inoculated with l. monocytogenes. power ultrasound (20 khz, 100% amplitude, 120 w) was applied for 30 or 60 s at a distance of 2.54 cm from a biofilm chip while it was submerged in 250 ml of sterile potassium phosphate buffer (ph 7.0). ozone was cycled through the 250 ... | 2009 | 19610346 |
| real-time pcr as a tool for detection of pathogenic bacteria on contaminated food contact surfaces by using a single enrichment medium. | foodborne pathogens such as escherichia coli o157:h7, listeria monocytogenes, and salmonella can easily transfer from food to food contact surfaces. methods for rapid detection of these pathogenic bacteria are important in order to avoid contamination of food. here, we describe the detection and quantification of bacterial pathogens on contaminated surfaces by real-time pcr (rt-pcr) and culture methods using a single enrichment medium. surfaces of wood, polypropylene, and stainless steel were in ... | 2009 | 19610347 |
| nonculturable response of animal enteropathogens in the agricultural environment and implications for food safety. | concerns about animal enteropathogen contamination of fresh horticultural products have,increased worldwide and are mainly due to the ability of bacteria to survive under stress conditions in the agricultural environment and during raw-vegetable processing. this review challenges the idea that the viable but nonculturable phenomenon that has been proven to occur in plant-associated environments contributes to human pathogen survival and might be correlated with foodborne infection. factors assoc ... | 2009 | 19610353 |
| listeriosis in pregnancy complicated by postpartum heart block. | listeria monocytogenes is a food-borne pathogen that primarily affects pregnant women. cardiac involvement is an uncommon complication of infection. we present a case of a gravida with listeria bacteremia at 36 weeks of gestation. | 2009 | 19622946 |
| efficacy of amoxicillin bearing microsphere formulation in treatment of listeria monocytogenes infection in swiss albino mice. | the present study deals with the evaluation of the efficacy of amoxicillin bearing poly-lactic-glycolic acid (plga) microsphere formulation in treatment of experimental listeriosis in swiss albino mice. amoxicillin bearing plga microspheres were prepared by water-in-oil-in-water emulsion technique. plga microwspheres significantly regulated sustained release of encapsulated drug over extended time period. the rate of release increased in temperature dependent manner. amoxicillin bearing plga mic ... | 2010 | 19624287 |
| efficacy of sodium hypochlorite and peracetic acid in sanitizing green coconuts. | to evaluate the efficacy of sanitizing green coconuts (cocos nucifera l.) through the treatment applied by juice industries using sodium hypochlorite and peracetic acid. | 2009 | 19627481 |
| antimicrobial activity of pomegranate (punica granatum l.) fruit peels. | food-borne diseases such as listeriosis and diseases caused by the emergence of multi-drug resistant pathogens (e.g. staphylococcus aureus) are globally recognized as environmental hazards to the food supply and human health. natural inhibitors for pathogenic microorganisms have been explored in many plants. the antimicrobial activity against some food-borne pathogens by various extracts from pomegranate fruit peels was evaluated using both in vitro (agar diffusion) and in situ (food) methods. t ... | 2009 | 19632734 |
| optimization of medium composition for the production of antimicrobial activity by bacillus subtilis b38. | an antimicrobial activity produced by bacillus subtilis b38 was found to be effective against several bacteria, including pathogenic and spoilage microorganisms such as, listeria monocytogenes, salmonella enteridis, and clinical isolates of methicillin-resistant staphylococcus species. nutrients such as carbon, nitrogen sources, and inorganic salts enhanced the production level of the antibacterial activity by b. subtilis b38. a first screening step showed that lactose, ammonium succinate, and m ... | 2009 | 19634172 |
| bacteriocin production by staphylococcus aureus involved in bovine mastitis in brazil. | in the present study, 257 staphylococcus spp. strains were isolated from bovine mastitis cases in 56 different brazilian dairy herds located in the southeast region of the country and tested for antimicrobial substance (ams) production. forty-six strains (17.9%) exhibited ams production and their identification as staphylococcus aureus was based on the presence of gram-positive cocci and on positive results in tests for the ability to coagulate rabbit plasma, to ferment mannitol, and to produce ... | 2009 | 19635553 |
| [development of single base extension-tags microarray for the detection of food-borne pathogens]. | we developed single base extension-tags (sbe-tags) microarray to detect eight common food-borne pathogens, including staphylococcus aureus, vibrio parahaemolyticus, listeria monocytogenes, salmonella, enterobacter sakazaki, shigella, escherichia coli o157:h7 and campylobacter jejuni. with specific pcr primers identified and integrated for eight food-borne pathogens, target sequences were amplified and purified as template dna of single base extension-tags reaction. the products were hybridized t ... | 2009 | 19637631 |
| occurrence of salmonella, campylobacter, yersinia enterocolitica, escherichia coli o157 and listeria monocytogenes in swine. | the objective of this study was to investigate the occurrence of major bacterial foodborne pathogens in swine. in total, 359 samples from manure storage tanks (91) and fresh pooled faeces (268) obtained from finisher (110), sows (78) and weanlings (80) were collected and tested. campylobacter, salmonella, yersinia enterocolitica, escherichia coli o157 and listeria monocytogenes were isolated from 36.5%, 31.5%, 5.8%, 3.3% and 3.3% of samples respectively. all e. coli o157 isolates found on 10 far ... | 2010 | 19638163 |
| characterisation of a small erythromycin resistance plasmid plfe1 from the food-isolate lactobacillus plantarum m345. | this paper reports the complete 4031 bp nucleotide sequence of the small erythromycin resistance plasmid plfe1 isolated from the raw-milk cheese isolate lactobacillus plantarum m345. analysis of the sequence revealed the coding regions for the erythromycin resistance determinant erm(b). a replication initiation protein repb was identified belonging to the repb proteins of the pmv158 family of rolling-circle replicating plasmids. the transcriptional repressor protein copg and a small counter tran ... | 2009 | 19640422 |
| quantitative phosphokinome analysis of the met pathway activated by the invasin internalin b from listeria monocytogenes. | stimulated by its physiological ligand, hepatocyte growth factor, the transmembrane receptor tyrosine kinase met activates a signaling machinery that leads to mitogenic, motogenic, and morphogenic responses. remarkably, the food-borne human pathogen listeria monocytogenes also promotes autophosphorylation of met through its virulence factor internalin b (inlb) and subsequently exploits met signaling to induce phagocytosis into a broad range of host cells. although the interaction between inlb an ... | 2009 | 19640851 |
| detection of enterobacter sakazakii and other pathogens associated with infant formula powder by use of a dna microarray. | pathogen detection is critical to the process of generating and testing powdered infant formula (pif). an obstacle associated with pif microbial surveillance is that most current procedures are time-consuming and labor-intensive. we have developed a rapid, dna microarray-based detection technique to identify 10 different pathogenic bacteria associated with pif contamination based on the 16s-23s rrna gene internal transcribed spacer (its) sequences and wzy (o antigen polymerase) gene. using this ... | 2009 | 19641057 |
| neutrophils sequestered in the liver suppress the proinflammatory response of kupffer cells to systemic bacterial infection. | the liver plays a major role in clearing bacteria from the bloodstream. rapid clearance is primarily the function of fixed tissue macrophages (kupffer cells) that line the hepatic sinusoids. although kupffer cells play a critical role in blood clearance, the actual elimination of the bulk of bacteria taken up by the liver depends upon the accumulation of bactericidal neutrophils. subsequent experiments demonstrating neutrophils inside kupffer cells derived from infected animals prompted our spec ... | 2009 | 19641138 |
| hypoxia-inducible factor-1alpha suppresses the expression of macrophage scavenger receptor 1. | macrophages are distributed in all peripheral tissues and play a critical role in the first line of the innate immune defenses against bacterial infection by phagocytosis of bacterial pathogens through the macrophage scavenger receptor 1 (msr1). within tissues, the partial pressure of oxygen (po2) decreases depending on the distance of cells from the closest o2-supplying blood vessel. however, it is not clear how the expression of msr1 in macrophages is regulated by low po2. on the other hand, h ... | 2009 | 19641936 |
| sigma(b) and sigma(l) contribute to listeria monocytogenes 10403s response to the antimicrobial peptides sdpc and nisin. | the ability of the foodborne pathogen listeria monocytogenes to survive antimicrobial treatments is a public health concern; therefore, this study was designed to investigate genetic mechanisms contributing to antimicrobial response in l. monocytogenes. in previous studies, the putative bacteriocin immunity gene lmo2570 was predicted to be regulated by the stress responsive alternative sigma factor, sigma(b). as the alternative sigma factor sigma(l) controls expression of genes important for res ... | 2009 | 19642919 |
| fulminant prosthetic valve endocarditis caused by listeria monocytogenes. | 2009 | 19643625 | |
| mechanism involved in phagocytosis and killing of listeria monocytogenes by acanthamoeba polyphaga. | intra-cellular pathogen, listeria monocytogenes, is capable of invasion and survival within mammalian cells. however, acanthamoeba polyphaga trophozoites phagocytose and rapidly degrade listeria cells. in order to provide more information on amoeba phagocytosis and killing mechanisms, this study used several inhibitor agents known to affect the phagocytosis and killing of bacteria by eukaryotes. amoebae were pre-treated with mannose, cytochalasin d, wortmannin, suramin, ammonium chloride, bafilo ... | 2009 | 19644706 |
| growth and reducing capacity of listeria monocytogenes under different initial redox potential. | to determine the reducing capacity of listeria monocytogenes and to highlight the effect of redox potential on its growth parameters. | 2010 | 19645768 |
| viability of listeria monocytogenes in co-culture with acanthamoeba spp. | listeria monocytogenes is a human pathogen, ubiquitous in the environment, and can grow and survive under a wide range of environmental conditions. it contaminates foods via raw materials or food-processing environments. however, the current knowledge of its ecology and, in particular, the mode of environmental survival and transmission of this intracellular pathogen remains limited. research has shown that several intracellular pathogens are able to survive or replicate within free-living amoeb ... | 2009 | 19645820 |
| survival of listeria monocytogenes, listeria innocua, and lactic acid bacteria in chill brines. | listeria monocytogenes is the pathogen of concern in ready-to-eat (rte) meat products. salt brines are used to chill processed meats. l. monocytogenes and lactic acid bacteria (lab) can grow under saline conditions, and may compete with each other for nutrients. the objective of this study was to determine the effect of lactic acid bacteria (enterococcus faecalis, carnobacterium gallinarum, and lactobacillus plantarum) on the survival of l. monocytogenes and listeria innocua in brines stored und ... | 2009 | 19646051 |
| c3 promotes expansion of cd8+ and cd4+ t cells in a listeria monocytogenes infection. | it is known that c3 is required for optimal expansion of t cells during acute viral infections. however, it is not yet determined whether t cell responses to intracellular bacterial infections require c3. therefore, we have investigated the requirement for c3 to elicit potent t cell responses to listeria monocytogenes (lm). we show that expansion of ag-specific cd8 and cd4 t cells during a primary response to lm was markedly reduced in the absence of c3 activity. further studies indicated that, ... | 2009 | 19648268 |
| estimating risk from small inocula by using population growth parameters. | risk from an uncertain small inoculum depends on variability of single-cell lag times. however, quantifying single-cell variability is technically challenging. it is possible to estimate this variability using population growth parameters. we demonstrate this possibility using data from literature and show a bayesian scheme for performing this task. | 2009 | 19648368 |
| competition of listeria monocytogenes serotype 1/2a and 4b strains in mixed-culture biofilms. | the majority of listeria monocytogenes isolates recovered from foods and the environment are strains of serogroup 1/2, especially serotypes 1/2a and 1/2b. however, serotype 4b strains cause the majority of human listeriosis outbreaks. our investigation of l. monocytogenes biofilms used a simulated food-processing system that consisted of repeated cycles of growth, sanitation treatment, and starvation to determine the competitive fitness of strains of serotypes 1/2a and 4b in pure and mixed-cultu ... | 2009 | 19648379 |
| listeria monocytogenes - from saprophyte to intracellular pathogen. | listeria monocytogenes is a bacterium that lives in the soil as a saprophyte but is capable of making the transition into a pathogen following its ingestion by susceptible humans or animals. recent studies suggest that l. monocytogenes mediates its saprophyte-to-cytosolic-parasite transition through the careful modulation of the activity of a virulence regulatory protein known as prfa, using a range of environmental cues that include available carbon sources. in this progress article we describe ... | 2009 | 19648949 |
| development and validation of a predictive model for listeria monocytogenes scott a as a function of temperature, ph, and commercial mixture of potassium lactate and sodium diacetate. | the objective of this study was to develop and validate secondary models that can predict growth parameters of l. monocytogenes scott a as a function of concentrations (0-3%) of a commercial potassium lactate (pl) and sodium diacetate (sda) mixture, ph (5.5-7.0), and temperature (4-37oc). a total of 120 growth curves were fitted to the baranyi primary model that directly estimates lag time (lt) and specific growth rate (sgr). the effects of the variables on l. monocytogenes scott a growth kineti ... | 2009 | 19652521 |
| effect of batch and fed-batch growth modes on biofilm formation by listeria monocytogenes at different temperatures. | the influence of listeria monocytogenes (l. monocytogenes) biofilm formation feeding conditions (batch and fed-batch) at different temperatures on biofilm biomass and activity was determined. biofilm biomass and cellular metabolic activity were assessed by crystal violet (cv) staining and 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2h-tetrazolium-5-carboxanilide inner salt (xtt) colorimetric method, respectively. live/dead staining was also performed in order to get microscopic visualization of the ... | 2009 | 19653035 |
| differential fluorescent staining of listeria monocytogenes and a whey food soil for quantitative analysis of surface hygiene. | the accurate monitoring of surface cleanliness in terms of bacterial contamination is usually carried out using methods such as plate counts or replica plating. however these methods take at least eighteen hours to obtain results and do not determine the presence or amount of residual organic material on a surface, which may interfere with cleaning and disinfection. this work describes the application of fluorescent stains to cells (listeria monocytogenes) and food soil (solubilized whey) to opt ... | 2009 | 19654071 |
| the risk of listeria monocytogenes infection in beef cattle operations. | to determine the prevalence of listeria monocytogenes and associated risk factors among beef operations (cow-calf and feedlot) in central and southern california. | 2010 | 19664067 |
| characterization of lactobacillus spp. isolated from the feces of breast-feeding piglets. | lactobacillus spp., referred to as ij-1 and ij-2, were isolated from the feces of breast-feeding piglets and analyzed for probiotic properties. according to the analyses of 16s rdna sequence, lactobacillus sp. ij-1 showed greater than 99% homology with lactobacillus reuteri dsm 20016(t), and lactobacillus sp. ij-2 had greater than 99% homology with the l. gasseri atcc 33323(t) and l. johnsonii atcc 33200(t). the ph changes in the culture media of lactobacillus sp. ij-1 and lactobacillus sp. ij-2 ... | 2009 | 19664551 |
| diagnosis of placental pathogens in small ruminants by immunohistochemistry and pcr on paraffin-embedded samples. | a histological study was carried out on 58 formalin-fixed and paraffin-embedded samples of placenta from sheep and goats that had aborted, and the placental lesions were graded. sequential histological sections of each cotyledon were then immunostained with specific antibodies and used for pcr detection of chlamydophila abortus, coxiella burnetii, salmonella abortusovis, brucella melitensis, listeria monocytogenes and toxoplasma gondii. most of the cotyledons showed different degrees of placenti ... | 2009 | 19666916 |
| recombinant listeria monocytogenes expressing a cell wall-associated listeriolysin o is weakly virulent but immunogenic. | listeriolysin o (llo) is an essential virulence factor for the gram-positive bacterium listeria monocytogenes. our goal was to determine if altering the topology of llo would alter the virulence and toxicity of l. monocytogenes in vivo. a recombinant strain was generated that expressed a surface-associated llo (sllo) variant secreted at 40-fold-lower levels than the wild type. in culture, the sllo strain grew in macrophages, translocated to the cytosol, and induced cell death. however, the sllo ... | 2009 | 19667043 |
| listeria marthii sp. nov., isolated from the natural environment, finger lakes national forest. | four isolates (fsl s4-120(t), fsl s4-696, fsl s4-710, and fsl s4-965) of gram-positive, motile, facultatively anaerobic, non-spore-forming bacilli that were phenotypically similar to species of the genus listeria were isolated from soil, standing water and flowing water samples obtained from the natural environment in the finger lakes national forest, new york, usa. the four isolates were closely related to one another and were determined to be the same species by whole genome dna-dna hybridizat ... | 2010 | 19667380 |
| obstetrician/gynecologists' knowledge, attitudes, and practices regarding prevention of infections in pregnancy. | maternal infection during pregnancy is a well-recognized cause of birth defects and developmental disabilities, as well as an important contributor to other adverse pregnancy outcomes. the objective of the present survey was to gain information about the knowledge, attitudes, and practices of obstetrician/gynecologists regarding prevention of infections during pregnancy. | 2009 | 19670963 |
| a multifactorial risk prioritization framework for foodborne pathogens. | we develop a prioritization framework for foodborne risks that considers public health impact as well as three other factors (market impact, consumer risk acceptance and perception, and social sensitivity). canadian case studies are presented for six pathogen-food combinations: campylobacter spp. in chicken; salmonella spp. in chicken and spinach; escherichia coli o157 in spinach and beef; and listeria monocytogenes in ready-to-eat meats. public health impact is measured by disability-adjusted l ... | 2010 | 19671103 |
| [molecular epidemiology of listeria monocytogenes isolates from imported fishery products to china]. | we examined the prevalence, molecular characteristics and virulence potential of listeria monocytogenes isolates from imported fishery products to gain further insights on the public health risk caused by this important food borne pathogen. | 2009 | 19673412 |
| disease presentation in relation to infection foci for non-pregnancy-associated human listeriosis in england and wales, 2001 to 2007. | listeriosis is a rare but severe food-borne disease, affecting unborn or newly delivered infants, the elderly, and the immunocompromised. the epidemiology of listeriosis in england and wales changed between 2001 and 2007, with more patients > or = 60 years old presenting with bacteremia (but without central nervous system [cns] involvement). in order to explain this increase and understand the altered disease presentation, clinical, microbiological, and seasonal data on bacteremic cases of liste ... | 2009 | 19675217 |
| heat and acid tolerance responses of listeria monocytogenes as affected by sequential exposure to hurdles during growth. | this study aimed to evaluate the effects of the level and sequence of hurdles, applied during growth, on the subsequent heat and acid tolerances of a 10-strain composite of listeria monocytogenes. individual strains were grown in glucose-free tryptic soy broth with 0.6% yeast extract (tsbye-g). then cultures were mixed and inoculated in fresh tsbye-g (0.5% nacl, ph 7.42; control), tsbye-g that was supplemented with 3% nacl (3.5% nacl in total), or tsbye-g with ph adjusted to 6.01 or 5.04 with la ... | 2009 | 19681263 |
| listeria monocytogenes in the irish dairy farm environment. | listeria monocytogenes is a potentially lethal foodborne pathogen commonly found in the environment. european union hygiene legislation places responsibility for safety on primary production facilities, including farms, as part of a policy to introduce traceability throughout the food chain. this study aimed to determine the occurrence of l. monocytogenes in the irish dairy farm environment and in particular the milking facility. two hundred ninety-eight environmental samples were collected from ... | 2009 | 19681268 |
| biofilm formation ability of listeria monocytogenes isolates from raw ready-to-eat seafood. | listeria monocytogenes is of great concern as a foodborne pathogen. many ready-to-eat foods are widely contaminated with this organism and have caused listeriosis outbreaks and sporadic cases in many countries. in japan, there is a high incidence of l. monocytogenes contamination, specifically in raw ready-to-eat seafood. identical l. monocytogenes subtypes have been isolated repeatedly from samples of food manufactured at a given store or processing plant, and researchers suspected that certain ... | 2009 | 19681273 |
| two-enzyme systems for glycolipid and polyglycerolphosphate lipoteichoic acid synthesis in listeria monocytogenes. | lipoteichoic acid (lta) is an important cell wall polymer in gram-positive bacteria and often consists a polyglycerolphosphate backbone chain that is linked to the membrane by a glycolipid. in listeria monocytogenes this glycolipid is gal-glc-dag or gal-ptd-6glc-dag. using a bioinformatics approach, we have identified l. monocytogenes genes predicted to be involved in glycolipid (lmo2555 and lmo2554) and lta backbone (lmo0644 and lmo0927) synthesis. lta and glycolipid analysis of wild-type and m ... | 2009 | 19682249 |
| toll-like receptors in bacterial meningitis. | bacterial meningitis is still an important infectious disease with a high morbidity and mortality rate. bacterial infection of the cerebrospinal fluid (csf) space causes a powerful inflammatory reaction that is largely responsibly for meningitis-induced tissue damage and adverse outcome of the disease. in a landmark series of experiments in the mid-1980s, cell wall components including lipooligosaccharides and lipoteichoic acid were indicated to be the key bacterial elements that can trigger the ... | 2009 | 19688326 |
| listeria monocytogenes meningitis in two immunocompetent children. | listeria monocytogenes is an uncommon cause of bacterial meningitis beyond the neonatal period. patients with immunosuppression or neoplastic disease are at increased risk of developing serious invasive disease, particularly meningitis. l. monocytogenes meningitis in two previously healthy, immunocompetent children aged 7 years and 18 months is described. one of them was successfully treated with ampicillin and amikacin. in the other there was resistance to ampicillin, and meropenem, vancomycin ... | 2009 | 19689866 |
| the key events dose-response framework: its potential for application to foodborne pathogenic microorganisms. | the key events dose-response framework (kedrf) is an analytical approach that facilitates the use of currently available data to gain insight regarding dose-response relationships. the use of the kedrf also helps identify critical knowledge gaps that once filled, will reduce reliance on assumptions. the present study considers how the kedrf might be applied to pathogenic microorganisms, using fetal listeriosis resulting from maternal ingestion of food contaminated with l. monocytogenes as an ini ... | 2009 | 19690997 |
| selectively reduced intracellular proliferation of salmonella enterica serovar typhimurium within apcs limits antigen presentation and development of a rapid cd8 t cell response. | ag presentation to cd8(+) t cells commences immediately after infection, which facilitates their rapid expansion and control of pathogen. this paradigm is not followed during infection with virulent salmonella enterica serovar typhimurium (st), an intracellular bacterium that causes mortality in susceptible c57bl/6j mice within 7 days and a chronic infection in resistant mice (129 x 1svj). infection of mice with ova-expressing st results in the development of a cd8(+) t cell response that is det ... | 2009 | 19692639 |
| screening of antibacterial activity of lactic acid bacteria against different pathogens found in vacuum-packaged meat products. | the objective of this work was to screen the antibacterial activity of lactic acid bacteria (lab) isolated from different sources against different pathogens found in ready-to-eat vacuum-packaged meat products (rte-vpmp). lab were isolated from human, rte-vpmp, fermented vegetables, and dairy samples. these isolates were assessed for their antibacterial activity against escherichia coli o157:h7, salmonella spp., listeria monocytogenes, and staphylococcus aureus using spot on lawn technique. six ... | 2009 | 19694551 |
| differential expression of proteins in listeria monocytogenes under thermotolerance-inducing, heat shock, and prolonged heat shock conditions. | listeria monocytogenes is a foodborne pathogen capable of employing stress adaptive responses to evade a variety of stressors including temperature stress. we employed two-dimensional gel electrophoresis coupled with matrix-assisted laser desorption/ionization-time of flight analysis to study the differential expression of l. monocytogenes (atcc 43256) soluble proteins at heat shock (60 degrees c) conditions, prolonged heat shock (60 degrees c for 9 minutes) conditions, and thermotolerance-induc ... | 2009 | 19694553 |
| principles, applications, and limitations of automated ribotyping as a rapid method in food safety. | automated ribotyping (ar) is a genotyping method used for identification and characterization of foodborne pathogens to the strain level. the advantages of ar are full automation, rapidity, and high reproducibility and typeability. ar may be a suitable characterization method for some pathogens when the research purpose requires a genotyping method with a strong discriminatory power. ar is a sensitive subtyping method for pathogens such as listeria monocytogenes and salmonellae, while its discri ... | 2009 | 19694556 |
| the role of natural killer cells in resistance to the intracellular bacterium listeria monocytogenes in rats. | we have investigated the influence of early innate immune resistance mechanisms on infection with the intracellular bacterium listeria monocytogenes in rats. rats were injected i.v. with various amounts of listeria and the number of bacterial colonies in the spleen was determined at different time points after infection. a bacterial dose as low as 2 x 10(4) cells gave reproducible infection within the spleen. athymic nude rats lacking normal t cells but with a robust nk cell repertoire for mhc a ... | 2009 | 19703013 |
| [impact of infection with listeria monocytogenes on the pregnancy]. | during a ten years period (06.1998a.-06.2007) at the delivery ward of maychin dom university hospital were admitted 31 627 parturients. among all of them we indentified and evaluated only 6 pregnant patients with listeria monocytogenes infection. during the first 8 years of the investigated period there was no even single case of the infection, whilst during the last 2 years the frequency of the listeria infection among all birth deliveries was 0.10%. the first five of our cases were admitted wi ... | 2007 | 19705692 |
| abhisin: a potential antimicrobial peptide derived from histone h2a of disk abalone (haliotis discus discus). | antimicrobial peptides (amps) play an important role in the immune defense against pathogenic microorganisms. in this study, a histone h2a full-length cdna was cloned from disk abalone haliotis discus discus. we identified a 40-amino acid amp designated as abhisin from the n-terminus of the abalone histone h2a sequence. abhisin shows the characteristic features of amps including net positive charge (+13), higher hydrophobic residues (27%) and 2.82 kcal/mol protein binding potential. abhisin shar ... | 2009 | 19706329 |
| combined effect of organic acids and supercritical carbon dioxide treatments against nonpathogenic escherichia coli, listeria monocytogenes, salmonella typhimurium and e. coli o157:h7 in fresh pork. | to evaluate the effectiveness of organic acids and supercritical carbon dioxide (sc-co(2)) treatments as well as their combined effect for the reduction of nonpathogenic escherichia coli and three pathogenic bacteria in fresh pork. | 2009 | 19709368 |
| effect of mixed antimicrobial agents and flavors in active packaging films. | active packaging is an emerging food technology to improve the quality and safety of food products. many works have been developed to study the antimicrobial activity of essential oils. essential oils have been traditionally used as flavorings in food, so they have an important odor impact but they have as well antimicrobial properties that could be used to protect the food. recent developments in antimicrobial active packaging showed the efficiency of essential oils versus bread and bakery prod ... | 2009 | 19711918 |