Publications

investigation into the antimicrobial action and mechanism of a novel endogenous peptide β-casein 197 from human milk.	a novel endogenous peptide cleaved from 197-213 aa of β-casein, named β-casein 197, was identified by tandem mass spectrometry. β-casein 197 constituted a significant proportion of the peptide content in preterm milk. this study investigated the antibacterial effects and mechanisms against common pathogenic bacteria. six bacterial strains were selected for this study: escherichia coli, staphylococcus aureus, yersinia enterocolitica, listeria monocytogenes, klebsiella pneumonia and bacillus subti ...	2017	28591979
application of long amplicon propidium monoazide-pcr to assess the effects of temperature and background microbiota on pathogens in river water.	the decay rates of enteric waterborne pathogens were evaluated following the introduction of yersinia enterocolitica, salmonella enterica, campylobacter jejuni and arcobacter butzleri into river water at different temperatures (5, 15 and 25°c) for a period of 28 days. to improve the accuracy of the results a molecular viability assay, long amplicon propidium monoazide-polymerase chain reaction (pma-pcr), was used to quantify the viable cell concentration and results from pcr with and without pma ...	2017	28598346
lactoferrin protects against intestinal inflammation and bacteria-induced barrier dysfunction in vitro.	the iron-binding glycoprotein lactoferrin (lf) is naturally present in human breast milk. several studies suggest that lf contributes to infant health and development owing to a variety of protective effects, including antimicrobial and anti-inflammatory features. therefore, we aimed to elucidate its protective properties on intestinal epithelial barrier dysfunction induced by infection or inflammation using the human epithelial cell culture models ht-29/b6 and t84. during barrier perturbation i ...	2017	28614589
x-ray crystal structures of the pheromone-binding domains of two quorum-hindered transcription factors, yenr of yersinia enterocolitica and cepr2 of burkholderia cenocepacia.	the ability of luxr-type proteins to regulate transcription is controlled by bacterial pheromones, n-acylhomoserine lactones (ahls). most luxr-family proteins require their cognate ahls for activity, and some of them require ahls for folding and stability, and for protease-resistance. however, a few members of this family are able to fold, dimerize, bind dna, and regulate transcription in the absence of ahls; moreover, these proteins are antagonized by their cognate ahls. one such protein is yen ...	2017	28614901
characterization of high exopolysaccharide-producing lactobacillus strains isolated from mustard pickles for potential probiotic applications.	the aim of this study was to characterize high exopolysaccharide (eps)-producing lactic acid bacteria (lab) isolated from mustard pickles in taiwan for potential probiotic applications. among 39 collected lab strains, four most productive eps-producing strains were selected for further analysis. comparative analyses of 16s rdna genes rpoa and phes sequences demonstrated that these strains were members of lactobacillus plantarum-group (lpg). ncd 2, nld 4, slc 13, and nld 16 showed survival rates ...	2017	28617525
green catanionic gemini surfactant-lichenysin mixture: improved surface, antimicrobial, and physiological properties.	catanionic surfactant mixtures form a wide variety of organized assemblies and aggregates with improved physicochemical and biological properties. the green catanionic mixture n(α)n(ω)-bis(n(α)caproylarginine) α,ω-propyldiamide (c3(ca)2):lichenysin (molar ratio 8:2) showed antimicrobial synergies against yersinia enterocolitica, bacillus subtilis, escherichia coli o157:h7, and candida albicans. flow cytometry and viability studies indicated that this catanionic mixture increases the probability ...	2017	28636319
emerging microbiota during cold storage and temperature abuse of ready-to-eat salad.	introduction: ready-to-eat (rte) leafy vegetables have a natural leaf microbiota that changes during different processing and handling steps from farm to fork. the objectives of this study were (i) to compare the microbiota of rte baby spinach and mixed-ingredient salad before and after seven days of storage at 8°c or 15°c; (ii) to explore associations between bacterial communities and the foodborne pathogens listeria monocytogenes, pathogenic yersinia enterocolitica, and pathogen model organism ...	2017	28649305
stand-alone eal domain proteins form a distinct subclass of eal proteins involved in regulation of cell motility and biofilm formation in enterobacteria.	the second messenger cyclic dimeric gmp (c-di-gmp) is almost ubiquitous among bacteria as are the c-di-gmp turnover proteins, which mediate the transition between motility and sessility. eal domain proteins have been characterized as c-di-gmp-specific phosphodiesterases. while most eal domain proteins contain additional, usually n-terminal, domains, there is a distinct family of proteins with stand-alone eal domains, exemplified by salmonella enterica serovar typhimurium proteins stm3611 (yhjh/p ...	2017	28652301
a dynamic and adaptive network of cytosolic interactions governs protein export by the t3ss injectisome.	many bacteria use a type iii secretion system (t3ss) to inject effector proteins into host cells. selection and export of the effectors is controlled by a set of soluble proteins at the cytosolic interface of the membrane spanning type iii secretion 'injectisome'. combining fluorescence microscopy, biochemical interaction studies and fluorescence correlation spectroscopy, we show that in live yersinia enterocolitica bacteria these soluble proteins form complexes both at the injectisome and in th ...	2017	28653671
the microbiota and autoimmunity: their role in thyroid autoimmune diseases.	since the 1970s, the role of infectious diseases in the pathogenesis of graves' disease (gd) has been an object of intensive research. the last decade has witnessed many studies on yersinia enterocolitica, helicobacter pylori and other bacterial organisms and their potential impact on gd. retrospective, prospective and molecular binding studies have been performed with contrary outcomes. until now it is not clear whether bacterial infections can trigger autoimmune thyroid disease. common risk fa ...	2017	28689782
development of a novel multiplexed qpcr and pyrosequencing method for the detection of human pathogenic yersiniae.	the purpose of this study was to develop a novel and robust molecular assay for the detection of human pathogenic yersiniae (i.e. yersinia enterocolitica, y. pseudotuberculosis and y. pestis) in complex food samples. the assay combines multiplexed real-time pcr (qpcr) and pyrosequencing for detecting and differentiating human pathogenic yersiniae with high confidence through sequence based confirmation. the assay demonstrated 100% specificity and inclusivity when tested against a panel of 14 y. ...	2017	28704728
secondary focal form of yersinia enterocolitica infection with prolonged polyarthritis in young caucasian male: a case report.	current issue deals with an interesting clinical case of a rare infectious disease in a caucasian young male patient, caused by yersinia enterocоlitica. infection proceeded in the development of secondary focal form, which was accompanied by prolonged polyarthritis. we described a clinical case of secondary focal form with prolonged polyarthritis caused by y. enterocolitica o:3 serogroup in young patient with the purpose of focusing on the early clinical and laboratory diagnosistics of yersinios ...	2017	28711901
galectin-1-driven tolerogenic programs aggravate yersinia enterocolitica infection by repressing antibacterial immunity.	yersinia enterocolitica is an enteropathogenic bacterium that causes gastrointestinal disorders, as well as extraintestinal manifestations. to subvert the host's immune response, y. enterocolitica uses a type iii secretion system consisting of an injectisome and effector proteins, called yersinia outer proteins (yops), that modulate activation, signaling, and survival of immune cells. in this article, we show that galectin-1 (gal-1), an immunoregulatory lectin widely expressed in mucosal tissues ...	2017	28716827
anti-adhesion and anti-biofilm potential of organosilane nanoparticles against foodborne pathogens.	nowadays, modification of surfaces by nanoparticulate coatings is a simple process that may have applications in reducing the prevalence of bacterial cells both on medical devices and food processing surfaces. to this direction, biofilm biological cycle of salmonella typhimurium, listeria monocytogenes, escherichia coli o157:h7, staphylococcus aureus, and yersinia enterocolitica on stainless steel and glass surfaces, with or without nanocoating was monitored. to achieve this, four different comm ...	2017	28744277
engineering of yersinia phytases to improve pepsin and trypsin resistance and thermostability and application potential in the food and feed industry.	susceptibility to proteases usually limits the application of phytase. we sought to improve the pepsin and trypsin resistance of yeappa from yersinia enterocolitica and ykappa from y. kristensenii by optimizing amino acid polarity and charge. the predicted pepsin/trypsin cleavage sites f89/k226 in pepsin/trypsin-sensitive yeappa and the corresponding sites (f89/e226) in pepsin-sensitive but trypsin-resistant ykappa were substituted with s and h, respectively. six variants were produced in pichia ...	2017	28752758
role of the transcriptional activator, virf, and temperature in the expression of the pyv plasmid genes of yersinia enterocolitica.	upon incubation at 37°c in the absence of ca(2+) ions, pathogenic yersiniae release high amounts of pyv plasmid-encoded proteins called yops, involved in pathogenesis. yersinia enterocolitica also express two outer membrane proteins, an adhesin called yada and a lipoprotein called yipa. the production of the yops is co-ordinately regulated by a 20kb region of the plasmid referred to as the 'ca(2+) dependence region' and containing at least four loci called vira, virb, virc, and virf. the 8.5kb v ...	1992	28776801
draft genome sequences of yersinia frederiksenii, yersinia intermedia, and yersinia kristensenii strains from brazil.	yersinia enterocolitica-like strains are usually understudied. in this work, we reported the draft genome sequences of two yersinia frederiksenii, two yersinia intermedia, and two yersinia kristensenii strains isolated from humans, animals, food, and the environment in brazil. these draft genomes will provide better molecular characterizations of these species.	2017	28798182
the antibacterial and antifungal activity of six essential oils and their cyto/genotoxicity to human hel 12469 cells.	six essential oils (from oregano, thyme, clove, lavender, clary sage, and arborvitae) exhibited different antibacterial and antifungal properties. antimicrobial activity was shown against pathogenic (escherichia coli, salmonella typhimurium, yersinia enterocolitica, staphylococcus aureus, listeria monocytogenes, and enterococcus faecalis) and environmental bacteria (bacillus cereus, arthrobacter protophormiae, pseudomonas fragi) and fungi (chaetomium globosum, penicillium chrysogenum, cladospori ...	2017	28811611
prevalence of yersinia enterocolitica bioserotype 3/o:3 among children with diarrhea, china, 2010-2015.	yersinia enterocolitica is thought to not significantly contribute to diarrheal disease in china, but evidence substantiating this claim is limited. we determined the prevalence of y. enterocolitica infection and strain types present among children <5 years of age with diarrhea in china. the overall prevalence of pathogenic isolates was 0.59%. prevalence of pathogenic bioserotype 3/o:3 varied geographically. in this population, the presence of fecal leukocytes was a characteristic of y. enteroco ...	2017	28820132