Publications

[infectious pneumopathies in immunodepressed patients. value of the study of bronchoalveolar lavage fluid].	during a period of 16 months 26 fluid specimens obtained by broncho-alveolar lavage (bal) in 24 immunocompromised patients were examined. this material included 13 hiv positive patients and 11 patients presenting malignant hemopathies (mh), of whom 7 had had a blood marrow transplantation. the bal fluid was divided into two equal parts, one of which was sent to the institute of pathology and the other to the laboratory of bacteriology of geneva university hospital. in some cases a transbronchial ...	1988	3175570
unusual clustering of allergic bronchopulmonary aspergillosis in children with cystic fibrosis.	allergic bronchopulmonary aspergillosis has been recognized in association with cystic fibrosis in children since 1965. since then, however, there have been a paucity of reports of pediatric cystic fibrosis complicated by allergic bronchopulmonary aspergillosis, and, in most cases, these have been diagnosed retrospectively. a cluster of five acute cases seen during a 4-month period in a single cystic fibrosis center with a systemic illness and deterioration in respiratory status are described. i ...	1988	3186372
5-( [aryl or aryloxy (or thio)]methyl)-3-(1h-imidazol-1-ylmethyl)-3- (2-thienyl)-2-methylisoxazolidine derivatives as novel antifungal agents.	the in vitro antifungal activity of a novel series of cis- and trans-5-([aryl or aryloxy (or thio)]methyl)-3-(1h-imidazol-1-ylmethyl)-3- (2-thienyl)-2-methylisoxazolidines (13-24) was evaluated and compared with ketoconazole. the title series of compounds was prepared via a 1,3-dipolar cycloaddition reaction of 1-(2-thienyl)-2-(1h-imidazol-1-yl)-n-methylethanimine n-oxides with appropriate styrenes, allyl phenyl ethers, or allyl phenyl thioether precursors. the resulting products were mixtures o ...	1988	3244110
immunoconjugate generation between the ribosome inactivating protein restrictocin and an anti-human breast carcinoma mab.	in the perspective of therapeutic approaches the monoclonal antibody, mbrl, with a quite restricted spectrum of reactivity for human breast carcinoma, was coupled to restrictocin (res), a ribosome inactivating protein produced by aspergillus restrictus. in a cell-free system this toxin was found to have an activity comparable to that of other plant toxins, but its in vitro toxicity was shown to be low on different cell lines. three batches of mbr1-res conjugate were prepared and their specificit ...	1988	3258791
allergic bronchopulmonary aspergillosis.	allergic bronchopulmonary aspergillosis is primarily an immunologic disorder manifested by episodic bronchial obstruction (asthma or chronic obstructive pulmonary disease). the host response to local aspergillus antigens gradually results in irreversible pulmonary injury. a high index of suspicion is required to make the diagnosis. early and aggressive treatment is important in preventing the serious consequences of central bronchiectasis, pulmonary fibrosis and cor pulmonale.	1988	3276099
aspergillus fumigatus infection of the maxillary sinus. a case report.	the diagnosis and radiological findings in a 48-year-old woman with paranasal sinus aspergillus fumigatus infection is described. the patient had had chronic post-nasal discharge and sinusitis for 7 years. she was treated by total removal of all infected material.	1988	3277293
partial characterization of a rapidly released antigenic/allergenic component (ag 5) of aspergillus fumigatus.	an antigenic component of aspergillus fumigatus, which was responsible for inducing an initial early antibody response in rabbits immunized with a. fumigatus germlings, has been identified and partially characterized. by immunofluorescent antibody and elisa techniques, this antigen was demonstrated to be present on the germling surface, although it was also detected in supernatants of conidia/germlings within 1 hour and was present in all shake and stationary culture-filtrate extracts. by incorp ...	1988	3279096
[primary empyema caused by aspergillus].		1988	3279277
necrotizing otitis externa due to aspergillus in an immunocompetent patient.	an 85-year-old, nondiabetic man is described with necrotizing (malignant) otitis externa. the patient developed facial nerve paralysis in the presence of a progressive cellulitis and chondritis of the external auditory meatus. destruction of the bony meatus and mastoid and obliteration of normal soft-tissue planes at the skull base were documented by computed tomographic scans. aspergillus fumigatus was isolated from the external meatus and deep tissue specimens. fungal invasion of bone and soft ...	1988	3281697
clinical and serological survey of pulmonary aspergillosis in patients with cystic fibrosis.	a 5.5-year survey revealed 9 patients with allergic bronchopulmonary aspergillosis (abpa) and 1 patient with a highly probable diagnosis among 200 danish patients with cystic fibrosis (cf). the incidence of abpa was 0.9 (0.4-1.7, 95% confidence limits) per 100 patients per year. all the patients with definite or probable abpa had serum antibodies to the aspergillus fumigatus catalase antigen, and in 5 patients the appearance of catalase antibodies during the incipient phase of abpa was documente ...	1988	3281913
aspergillosis of the nose and paranasal sinuses in immunocompromised children.		1988	3284628
assessment of immunoblotting in the investigation of specific immune responses during infection.		1988	3286316
[allergic bronchopulmonary aspergillosis with hay dust-induced alveolitis and bronchial asthma].	a 55-year-old female farmer had all the clinical, biochemical and radiological signs diagnostic of allergic bronchopulmonary aspergillosis. remarkably she also had symptoms of farmer's lung and exogenous allergic bronchial asthma with specific types i and iii sensitisation due to aspergillus antigens inhaled with straw-dust. in addition to the usual asthma treatment she also received antimycotic agents and corticosteroids. antigen exposure was strictly avoided. these measures brought about lasti ...	1988	3292187
isolation and characterization of a relevant aspergillus fumigatus antigen with igg- and ige-binding activity.	an immunologically relevant antigen fraction was isolated from a cytoplasmic extract of aspergillus fumigatus mycelium. the fraction was obtained by concanavalin-a affinity chromatography and hydrophobic interaction chromatography. two protein bands were discernible in polyacrylamide gel electrophoresis while two precipitin peaks were detected in crossed immunoelectrophoresis. when tested against sera from patients with aspergillus-induced diseases, the fraction showed both igg and ige antibody- ...	1988	3292438
factors affecting protoplast release from some mesophilic, thermophilic and thermotolerant species of filamentous fungi using novozym 234.	factors affecting the release of protoplasts from mycelia of aspergillus fumigatus, aspergillus niger, penicillium ochrochloron and geosmithia emersonii were investigated. protoplast yields were found to be greatly dependent on the growth temperature, incubation conditions, lytic enzyme system, mycelial age and mycelial concentration. high yields of protoplasts were obtained from all four organisms after a 3 h incubation with 1 mg/ml novozym 234 in 0.1 m 2-n-[morpholino] ethane sulphonic acid (m ...	1988	3292876
in vitro activity of enilconazole against aspergillus spp. and its fungicidal efficacy in a smoke generator against aspergillus fumigatus.		1988	3292911
[pulmonary and cerebral aspergillosis in burkitt's lymphoma].	multiple intracerebral aspergillus abscesses in a 5 year old boy with a burkitt's lymphoma are described. the disease was fatal despite antifungal treatment. the diagnostic and therapeutic problems, the risk factors and preventive care are discussed.	1988	3293010
[invasive pulmonary aspergillosis].	invasive pulmonary aspergillosis is an opportunistic pneumonia which is particularly common in immuno-depressed subjects. the most frequent risk factors are the intensity and duration of leucopaenia, cytotoxic drugs and steroid therapy. in the respiratory tract, the proliferation of aspergillus fumigatus becomes possible when there is a failure of the reticulo-endothelial system and of the polymorphonuclear leucocytes. there is no typical clinical picture: one sees an acute febrile pneumonia res ...	1988	3293130
[histological study of a case of chondrocostal aspergillosis].	histological study of biopsy specimens successively taken from the 7th, 5th and 6th right chondrocostal regions in a 33 year-old man; the patient was under chemotherapy for a diffuse lymphoma and has been operated for an aspergilloma of the right lung superior lobe. non-specific chronic osteomyelitis was observed as well as aspergillus fumigatus perichondritis (acute and chronic) which had lead to cartilage fragmentation. cartilage fragmentation could be attributed to an enzymatic chondrolysis i ...	1988	3293580
aspergillus endocarditis in association with a false aortic aneurysm.	a 32-year-old man was admitted with an intracerebral hematoma and subsequently with bilateral femoral emboli five months after aortic valve replacement. blood cultures for bacteria and fungi were negative but microscopy of the embolus revealed aspergillus fumigatus. at operation a large false aneurysm of the ascending aorta and vegetations on the prosthetic aortic valve were found. aspergillus endocarditis is diagnosed antemortem in only 23% of fatal aortic aneurysms: blood cultures are positive ...	1988	3293861
fatal aspergillosis associated with smoking contaminated marijuana, in a marrow transplant recipient.	a 34-year-old man presented with pulmonary aspergillosis on the 75th day after marrow transplant for chronic myelogenous leukemia. the patient had smoked marijuana heavily for several weeks prior to admission. cultures of the marijuana revealed aspergillus fumigatus with morphology and growth characteristics identical to the organism grown from open lung biopsy specimen. despite aggressive antifungal therapy, the patient died with disseminated disease. physicians should be aware of this potentia ...	1988	3293934
detection of galactomannan antigenemia by enzyme immunoassay in experimental invasive aspergillosis.	a sensitive enzyme immunoassay (eia) for galactomannan antigenemia that avoids the use of radioisotopes was devised. three carbohydrate-rich antigenic fractions were purified from aspergillus fumigatus 2085: a cold alkali extract (ca) from mycelium, an acetone-precipitated pyridine extract (apsk-66) from mycelium, and a methanol precipitate from culture filtrate. ca and apsk-66 were further purified by gel filtration and ion-exchange chromatography, respectively. an acid hydrolysate of ca contai ...	1987	3294887
fungal infections in birds in captivity--six case reports.		1987	3295539
fatal hemoptysis in a patient with aids-related complex and pulmonary aspergilloma.		1987	3295611
the synchronous occurrence of aspergillosis and myospherulosis of the maxillary sinus. report of a case with review of the literature.		1987	3295652
rapid diagnosis of candidiasis and aspergillosis.	published studies support the hypothesis that at least two antigens of candida albicans and candida tropicalis circulate in the bloodstream of patients with severe candidiasis. one antigen (probably mannan) is stable and the other (probably protein) is labile. the stabile antigen can rarely be detected without prior dissociation from antibody. dissociation treatment destroys the labile antigen, leaving in doubt whether any is antibody bound. dissociation steps have also been necessary for detect ...	1987	3296104
[aspergillosis in wild geese (chloëphaga poliocephala scl., 1857) in chile].		1987	3296570
[allergic bronchopulmonary aspergillosis].	the article concerns one of the present-day problems in the teaching of the allergic diseases of the lungs, namely allergic bronchopulmonary aspergillosis. discusses the disease diagnosis and clinical course, diagnostic approaches, efficient drug therapy, and prognostic aspects. based on an analysis of the authors' own data, the problem of the possibility of the systemic pathoimmune manifestations associated with skin, eye and kidney injuries that occur during allergic bronchopulmonary aspergill ...	1987	3299854
quantitative neutralization assay of fungicidal activity of disinfectants.	a quantitative assay using a neutralization medium was developed to evaluate fungicidal activity of disinfectants. concentrated dey-engley neutralizing broth was used in this study and was demonstrated to inactivate various chemical agents within 5 min when disinfectant concentrations were reduced to specific levels. addition of this dey-engley broth to test tubes containing fungal cells and disinfectants permitted control of the various interactions times. subsequent concentration of the disinf ...	1987	3300541
inhibition of germ tube development of aspergillus fumigatus in cell-free transudate produced in subcutaneous chambers in rabbits.	the interaction of aspergillus fumigatus conidia with host factors produced in rabbits was studied by means of subcutaneous, perforated plastic chambers. transudate fluid from spore-free chambers, sampled 30 days after implantation, supported germ tube development and rapid hyphal growth of a. fumigatus in an assay in vitro. inoculation of spores into chambers implanted 30 days previously produced a rapid infiltration of leukocytes, predominately neutrophils, into the chamber fluid. cell-free su ...	1987	3302197
synthesis and antifungal activity of a series of novel 1,2-disubstituted propenones.	to find an antifungal agent other than those of the imidazole and triazole series, a new class of 1,2-disubstituted propenones i and ii was prepared and tested for antifungal activity. comparison of the structure-activity relationships showed that the conjugated structure of carbonyl and exomethylene groups in i and ii plays an important role in potent antifungal activity. however, it is noteworthy that compounds 53, 54, and 56, which have a hydroxymethyl or methoxymethyl group instead of an exo ...	1987	3302258
invasive bronchopulmonary aspergillosis leading to rapidly progressive respiratory failure in a patient with severe aplastic anemia.	a 22-year-old man with severe aplastic anemia was treated with antilymphocyte globulin, prednisone, and oxymetholone. fourteen days after initiation of treatment he developed a fulminant mediastinal and subcutaneous emphysema leading to respiratory failure refractory to mechanical ventilation. fiberoptic bronchoscopy showed nodular lesions typical of aspergillus. cultures of bronchial mucus revealed aspergillus fumigatus as the responsible pathogen.	1987	3302517
an outbreak of invasive aspergillosis in a haematologic unit.	within a period of 15 months 8 cases of invasive pulmonary aspergillosis were seen in a haematologic unit; 7 of them between january and october 1984. no previous cases of invasive aspergillosis had been encountered during the existence of the unit since 1979. environmental studies did not prove a single likely source for the fungal spores. previous window renovation with concomitant fiber deposits on ventilation grids, poor sealing of air filter fittings in patient rooms, occasional ventilation ...	1987	3303306
trial of ketoconazole in non-invasive pulmonary aspergillosis.	a one year study of the efficacy of the antifungal agent ketoconazole in non-invasive pulmonary aspergillosis was carried out. ten patients, seven with allergic bronchopulmonary aspergillosis and three with mycetoma, were studied. they were randomly allocated to receive 400 mg daily or placebo orally in a double blind fashion. in the treated group (n = 6), concentrations of serum igg specific for aspergillus fumigatus fell significantly during treatment (mean reduction 42% (sem 2.2%) compared wi ...	1987	3303424
fumifungin, a new antifungal antibiotic from aspergillus fumigatus fresenius 1863.		1987	3305454
right occipital cerebral abscess caused by aspergillus fumigatus.	a rare case of intracerebral abscess caused by aspergillus fumigatus located in the right occipital region is described; pathogenesis, prognosis and treatment of this particular infection are discussed.	1987	3305803
[diagnosis of invasive pulmonary aspergillosis].		1987	3306195
pleural aspergillosis diagnosed by computerized tomography.	as shown by the case we have reported, patients with a chronic pneumothorax and nodular, thickened pleura may have pleural aspergillosis. we recommend that when such patients have ct of the chest, the examiner use the alternate dependent position to look for mobile pleural debris.	1987	3306949
release of antigens and allergens during shake-culture of aspergillus fumigatus.	the appearance of igg and ige binding components in the medium of shake cultures of aspergillus fumigatus has been studied. cultures were grown in synthetic asparagine medium at 35 degrees c and flasks harvested in duplicate 1, 2, 3, 4, 7 and 14 days after inoculation. the ph of the medium dropped from its initial value of 5.5 to ph 3, and then after 4 days gradually increased up to ph 7.5 in the 14-day medium. the weight of mycelium, after an initial peak followed by a slight decline, increased ...	1987	3307516
disseminated aspergillosis in a patient with ocular reticulum cell sarcoma.	a 75-year-old healthy woman developed uveitis and chorioretinal infiltrates od compatible with reticulum cell sarcoma. she was admitted to hospital 18 months later with somnolence and confusion. examination revealed diffuse white matter hypodensity on computed tomography and a persistent cerebrospinal fluid pleocytosis. at necropsy reticulum cell sarcoma was found in the right globe and aspergillus fumigatus was present in almost every other organ, including the brain.	1987	3307899
resistance of spores of aspergillus fumigatus to ingestion by phagocytic cells.	phagocytic cells are believed to have an important role in the eradication of fungal spores from the lung. the ability of human and mouse cells to phagocytose the opportunistic fungus aspergillus fumigatus has been examined, spores of the non-pathogenic fungus penicillium ochrochloron being used for comparison. most spores became associated with cells. those of a fumigatus appeared to remain bound to the surface of the phagocyte rather than being ingested; in contrast, p ochrochloron spores appe ...	1987	3310309
invasive pulmonary aspergillosis diagnosed by blood culture and successfully treated.	we present a case of invasive pulmonary aspergillosis when a blood culture isolate of aspergillus fumigatus in an unusual medium was considered to be a genuine isolation. early institution of appropriate therapy was successful.	1987	3311121
immune responses in experimental keratitis by aspergillus fumigatus fresenius.		1987	3311979
serologic response to candida albicans and aspergillus fumigatus in cystic fibrosis.	in order to evaluate the role of fungi in patients with cystic fibrosis, we determined serum igg antibodies against candida albicans and aspergillus fumigatus using an indirect elisa in patients with or without fungi in the sputum and in sera of healthy individuals. for both c. albicans and a. fumigatus the antibody levels were significantly higher in the cf groups than in the control group, regardless of whether these fungi could be isolated during the observation time or not. in contrast to a. ...	1987	3312024
electrophoretic and serological analyses of cytoplasmic antigens from aspergillus fumigatus during growth of conidia to mature mycelia.	the changes of cytoplasmic components concomitant with conidium to mature mycelium growth of aspergillus fumigatus strain ag 507 were analysed by one- and two-dimensional polyacrylamide gel electrophoresis (sds-page; 2-de). sds-page monitored molecular weight differences between components of cytosol preparations obtained from conidia and those through 96 h of mycelial growth. 2-de analyses indicated that some components characteristic of mature cytosol begin to appear by 7 h. cytoplasmic prepar ...	1987	3312558
[aspergillus pneumonia in patients with chronic bronchopathy. presentation of 3 cases].		1987	3312858
isolated cardiac aspergillosis after bone marrow transplantation.	a 15-year-old girl developed isolated cardiac aspergillosis after allogeneic bone marrow transplantation. predisposing factors included severe graft-vs-host disease with gastrointestinal tract involvement and immunosuppressive therapy including cyclosporine. the patient died of cardiopulmonary failure 11 weeks after transplantation.	1987	3314764
prolonged evaluation of patients with corticosteroid-dependent asthma stage of allergic bronchopulmonary aspergillosis.	eight cases with stage iv allergic bronchopulmonary aspergillosis (abpa) (corticosteroid-dependent asthma stage) were observed for a total of 82 patient years with individual patients observed for 7 to 19 years (mean 10.2) years. one case is the first case of abpa diagnosed in the united states in 1967. a second case has been observed through four stages of abpa. none of these eight cases has demonstrated pulmonary deterioration by clinical, chest roentgenogram, or pulmonary function analysis. a ...	1987	3316345
[endocarditis caused by aspergillus fumigatus].		1987	3316873
invasive pulmonary aspergillosis: radiologic evidence of tracheal involvement.	chest radiographs of invasive pulmonary aspergillosis in immunocompromised patients typically show ill-defined pulmonary nodules. direct tracheal involvement has not been previously documented radiologically. radiographs and ct scans of a 16-year-old boy with aplastic anemia and invasive pulmonary aspergillosis showed increased paratracheal opacity, tracheal narrowing, and pneumomediastinum in combination with pulmonary disease. bronchial lavage cultures grew aspergillus fumigatus, and the boy d ...	1987	3317503
chip pile workers and mould exposure. a preliminary clinical and hygienic survey.	chip pile workers are exposed to high concentrations of airborne mould spores. few sporadic cases of allergic alveolitis have been reported in finland. eight non-smoking workers, six on chip piles and two on bark piles, were examined to study the immunological and clinical response to continuous mould spore exposure. the spore exposure ranged between 10(4) and 10(5) spores/m3. three of the six chip pile workers had precipitating serum antibodies against aspergillus fumigatus. the three antibody- ...	1987	3318540
characterization of a second major antigen ag 13 (antigen c) of aspergillus fumigatus and investigation of its immunological reactivity.	a second major antigen of aspergillus fumigatus (ag 13) has been identified and characterized as a 70 kd, heat-labile component which is able to bind to the lectin, concanavalin a. ag 13 proved to be identical to the previously recognized c antigen, known to possess chymotryptic activity. a monospecific antiserum to ag 13 has been affinity purified and used to develop a sandwich elisa for measuring levels of ag 13 specific igg antibodies in patients' sera. although all abpa sera (25/25) had sign ...	1987	3319301
invasive pulmonary aspergillosis: a diagnostic and therapeutic problem. clinical experience with eight haematologic patients.	eight patients with haematologic malignancies contracted fatal invasive aspergillosis during an outbreak. five patients were neutropenic. bronchofiberoscopic examination with microbiology specimen brush and bronchoalveolar lavage yielded aspergillus fumigatus in only 2/5 patients examined. the specific diagnosis reached during lifetime in 5 patients was based on a combination of invasive procedures (lung biopsy in 2, percutaneous lung puncture in 1), the presence of a lung abscess (3 patients), ...	1987	3321414
pathogenetic and serological aspects of pulmonary aspergillosis.	af is an important pathogen of the bronchopulmonary system, and the clinical spectrum encompasses aspergilloma, cnpa, ipa, abpa, bronchial asthma, and allergic alveolitis. bronchial carriage may, however, not always be associated with pathological effects. the polymorphism of the aspergillus-related disorders seems mostly to depend upon the different responses of the hosts. this review considers the antigenic composition of af and specific antibody responses in man in relation to the pathogenesi ...	1987	3321416
hematogenously acquired aspergillus vertebral osteomyelitis in seemingly immunocompetent drug addicts.		1987	3321709
evaluation of a test to detect circulating aspergillus fumigatus antigen in a survey of immunocompromised patients with proven or suspected invasive disease.	an elisa for the detection and measurement of aspergillus antigenaemia has been developed and evaluated by examining sera submitted over a 12-month period from immunocompromised patients with a likelihood of invasive aspergillosis. results from proven cases of invasive aspergillosis confirmed at post-mortem and specimens from individuals with suspected disease showed that tests on single serum samples were often negative. multiple specimens from the same patient greatly increased the frequency o ...	1987	3325629
inhibition of phagocyte migration and spreading by spore diffusates of aspergillus fumigatus.	previous studies have shown that spores of aspergillus fumigatus inhibit phagocytosis and killing by macrophages and polymorphonuclear leucocytes. in order to identify the mechanisms of this interference with host defences, we have examined the effects of a. fumigatus spore diffusates on phagocytic cell function. for comparison, we have used spore diffusates of the non-pathogenic fungus penicillium ochrochloron. the diffusates of a. fumigatus reduced the number of human polymorphonuclear leukocy ...	1987	3325630
aspergillosis of the brain: report of an autopsy proven case.		1987	3327911
pulmonary berylliosis on corticosteroid therapy, with cavitating lung lesions and aspergillomata--report on a fatal case.	a fatal case of pulmonary berylliosis in a 42 year old male is described. the patient was exposed to beryllium while working in a chemical plant over a 9 year period, and presented two years after ceasing such employment. the berylliosis was diagnosed on open lung biopsy in 1971. the patient was commenced on steriod therapy at that time. he suffered progressive dyspnoea from severe restrictive lung disease over the next 14 years. a chest x-ray of june 1985 revealed a lesion in the left upper lob ...	1987	3328191
biochemical and immunochemical analyses of detergent solubilized antigens from membrane vesicles of aspergillus fumigatus.	a membrane vesicle fraction isolated from exponentially growing aspergillus fumigatus strain ag 507 cultures was obtained by mechanical disruption of intact aspergillus cells under specific osmotic conditions followed by a ph fractionation technique. electron micrographs of the membrane vesicles indicated unit membrane structures free from cell wall material. high glucose-6-phosphatase and low lactate dehydrogenase activities verified the relative purity of the membrane vesicle fraction. allergi ...	1987	3329002
a case of aspergillosis of the maxillary sinus.		1987	3329218
[allergic bronchopulmonary aspergillosis].		1987	3330331
[studies on the mechanism of developing bronchial asthma due to candida albicans. 5. a comparative study of the mechanisms producing bronchial asthma among candida, other molds and house dust-induced asthma].		1987	3330418
[methods of inducing experimental corneal mycosis].		1987	3330743
[pathogenic properties of aspergillus fumigatus fres].		1987	3334261
[experimental fumigatotoxicosis in mink].		1987	3334269
fungal mastoiditis in the immunocompromised host.	an immunocompromised patient is subject to unusual, severe opportunistic infections. we report a case of aspergillus fumigatus mastoiditis in a patient with leukemia who also had cryptococcal meningitis. a fatal outcome ensued, despite extensive surgical and antimicrobial intervention.	1988	3337779
allergic asthma in different population groups in the western cape. causative and complicating factors.	allergic asthma is a disease with a well-defined aetiology, the recognition and elimination of which could be achieved with relatively simple and inexpensive prophylactic treatment. some of the well-known factors--respiratory tract infections, exposure to cigarette smoke, specific antigens and regular application of prophylactic treatment--which could cause or complicate asthma were studied in groups of white and coloured patients. more respiratory tract infections occurred in coloured patients ...	1988	3340935
skin testing with extracts of fungal species derived from the homes of allergy clinic patients in toronto, canada.	airborne fungi in the homes of patients with allergic rhinitis or asthma, from a toronto allergy clinic population, were isolated, quantified and identified to species. allergen extracts were prepared from sixteen of these isolated species and used for skin-prick testing of twenty-six patients. fourteen of the total patients reacted to one or more of these extracts at 1:10 (w/v) concentrations. the most common positive skin responses (8/14 to 6/14) were found for cladosporium cladosporioides, al ...	1988	3349592
microbiology of indwelling central intravascular catheters.	indwelling central-line catheters (n = 502) from 362 patients in intensive care units were analyzed prospectively. the skin site, 6-cm-distal and -proximal subcutaneous segments of the catheter, exudates, and blood were cultured. semiqualitative roll plate cultures of the catheter segments were followed by broth cultures and examined for 72 h. all isolates were identified, and susceptibilities were determined. line infections, defined clinically, yielded 22 different microbial species; 10 differ ...	1988	3366864
aspergillus rhinitis in wistar (crl:(wi)br) rats.	in two separate 24 month studies on the carcinogenic effect of single cadmium chloride injections in male wistar (crl:(wi)br) rats, a total of 22% (129/597) of animals studied histologically were found to have chronic suppurative rhinitis caused by aspergillus fumigatus. the diagnosis was based on characteristic conidial heads present in the sections, and positive methenamine-grocott (gms) staining of septate hyphae with dichotomous branching at angles of 45 degrees. fungal hyphae balls, surroun ...	1988	3374092
immune responses to aspergillus in cystic fibrosis.	aspergillus fumigatus (af) is well recognized in its ability to colonize the respiratory tract in cystic fibrosis (cf). furthermore, a number of the immune responses of the patient with cf to this organism have been characterized, and the immune inflammatory response to af may result in allergic bronchopulmonary aspergillosis (abpa). this study evaluated a series of immunologic parameters in 75 patients with cf in order to characterize more fully the spectrum of immune responses of those patient ...	1988	3392373
disseminated strongyloides stercoralis and aspergillus fumigatus presenting as diffuse interstitial pneumonitis in a steroid-dependent chronic obstructive pulmonary disease patient.		1988	3397296
aflatoxins and fungal flora in lentil (lens esculenta l.).	fungal flora of 20 samples of lentil seeds collected from assiut governorate, egypt, were studied. seventeen genera and 13 species were isolated on glucose- (15 genera and 27 species), cellulose- (15 genera and 25 species)- czapek's agar media at 28 degrees c. the most common species were as follows: on glucose-czapek's agar, aspergillus fumigatus, a. niger, a. flavus, a. terreus, penicillium notatum and rhizopus oryzae and on cellulose agar, a. fumigatus, a. niger, a. flavus and p. notatum. thi ...	1988	3419482
cystic fibrosis in adolescents and adults.	three hundred and sixteen patients with cystic fibrosis were seen at the brompton hospital during 1965-83; 178 (56.3%) of them were male and 136 female, and their ages ranged from 12 to 51 years. most patients presented in infancy with respiratory symptoms and malabsorption, but 19 (6%) were diagnosed in adult life, three in their 30s. pulmonary disease was almost universal (99.7%), being responsible for 97% of all deaths and three quarters of hospital admissions. all patients had developed a pr ...	1987	3438896
precipitins against microbes in mouldy hay in the sera of farmers with farmer's lung or chronic bronchitis and of healthy farmers.	the aim of this study was to determine which precipitins against four antigens in 2,440 farmers are associated with the occurrence of farmer's lung (fl). the antigen panel consisted of mycelial antigens of micropolyspora faeni, thermoactinomyces vulgaris, aspergillus fumigatus and aspergillus umbrosus. as reference groups we used healthy farmers and those with chronic bronchitis. for the occurrence of precipitins against the four antigens there was a statistically significant difference between ...	1987	3478211
rast-specific ige in nigerian asthmatic patients.	this first study of the pattern of allergen-specific ige in nigerian asthmatic patients in zaria shows a significant percentage with specific ige to dermatophagoides pteronnyssinus, aspergillus fumigatus, and cynodon dactylon (bermuda grass). total serum ige varied in both urban and rural asthmatics with a pattern of low and high levels in all groups.	1986	3484920
corneal biopsy in the diagnosis of keratomycosis.	in two patients, a 55-year-old man and a 49-year-old man, who had fungal keratitis initially undiagnosed by corneal scrapings the condition was successfully diagnosed by corneal biopsy. we compared corneal biopsy specimens and corneal scraping in the diagnosis of keratomycosis in rabbits with experimental bilateral fungal keratitis caused by fusarium solani, aspergillus fumigatus, and candida albicans. corneal scrapings disclosed three specimens (30%) positive for candida, five (50%) for fusariu ...	1986	3485380
activation of human serum complement with allergens. i. generation of c3a, c4a, and c5a and induction of human neutrophil aggregation.	to understand the relevance of allergens in generation of c3a, c4a, and c5a in normal human serum, we studied extracts of several allergens (house dust, house dust mite, aspergillus fumigatus, and perennial ryegrass). known complement activators zymosan and endotoxin were used as controls. generation of c3a, c4a, and c5a (determined by radioimmunoassays) occurred with extracts of house dust and aspergillus more than with house dust mite and ryegrass. anaphylatoxins were produced both in dose and ...	1987	3496373
serum precipitins against microbes in mouldy hay with respect to age, sex, atopy, and smoking of farmers.	this study was based on a sample of 3,065 farmers from a larger survey population of 12,056 finnish farmers. data were collected in a postal survey conducted by the social insurance institution of finland. serum samples for determination of precipitating antibodies were taken at local health centres. precipitins were determined by the method of microplate immune diffusion. the antigen panel consisted of mycelial antigens of micropolyspora faeni, thermoactinomyces vulgaris, aspergillus fumigatus, ...	1987	3499339
serum precipitins against microbes in mouldy hay with respect to the geographical location of the farm and to the work of farmers.	this study was based on a sample of 3,065 farmers from a larger survey population of 12,056 finnish farmers. data were gathered in a postal survey conducted by the social insurance institution of finland. serum precipitins were determined by a microplate method of immune diffusion. the antigen panel consisted of mycelial antigens of micropolyspora faeni, thermoactinomyces vulgaris, aspergillus fumigatus, and aspergillus umbrosus. geographical variation in the prevalence of precipitins was statis ...	1987	3499340
synthesis and structure-activity relationships of naftifine-related allylamine antimycotics.	naftifine (1) is the first representative of the new antifungal allylamine derivatives. its biological activity is strictly bound to specific structural requirements that are unrelated to those of known antifungals. a tertiary allylamine function seems to be a prerequisite for activity against fungi. by systematic variation of the individual structural elements in 1, detailed structure-activity relationships are defined in which the phenyl ring is the structural feature permitting the widest var ...	1986	3510297
head and neck aspergillosis in patients undergoing bone marrow transplantation. report of four cases and review of the literature.	aspergillus infection can be a major cause of morbidity in immunocompromised patients, especially when there is pulmonary involvement. diagnosis of aspergillosis is often complicated by the varied clinical presentation and compromised medical status of such patients. four cases of head and neck aspergillus infections in bone marrow transplant patients are presented. involvement predominantly was limited to the oral cavity and/or sinuses, and in one case, the ear. two cases were successfully mana ...	1986	3510710
[mycotic placentitis in cattle].	two cases of bovine mycotic placentitis are reported. the two cows did not show any clinical symptoms before or during premature delivery of two clinically normal calves. in both placentas a severe placentitis accompanied by extensive necrosis and a large number of hyphae was present in two placentas. aspergillus fumigatus was isolated in one case. the literature on bovine mycotic placentitis is reviewed in the discussion.	1986	3511563
studies on the association of aspergillus fumigatus with ocular infections in animals.	the occurrence and aetiological significance of aspergillus fumigatus, an opportunistic pathogen, have been studied in 93 animals with various ophthalmological problems. a total of 93 eye swabs collected from 35 mules, 26 dogs, 13 fowl, 11 cattle, five buffaloes and three camels were investigated mycologically for the presence of a fumigatus. the pathogen was isolated in pure and heavy growth from the swabs from two dogs, one bull, one mule and one fowl. the fungus was also demonstrated directly ...	1986	3511605
spinal arachnoiditis due to aspergillus meningitis in a previously healthy patient.	a 30-year-old, previously healthy, non-addicted man presented with a chronic spinal meningitis complicated by arachnoiditis and spinal cord compression. biopsy showed a chronic granulomatous leptomeningitis, in which some cells contained branching septate organisms that were immunostained with an antiserum to aspergillus fumigatus. precipitins to a. fumigatus were detected in cerebrospinal fluid (csf), but not in blood, and aspergillus infection was apparently restricted to the leptomeninges. cl ...	1986	3512781
binding of c-reactive protein to aspergillus fumigatus fractions.	calcium-dependent binding of c-reactive protein (crp) to aspergillus fumigatus was determined by enzyme-linked immunosorbent assay. a homogenate of young hyphae was fractionated by hydrophobic interaction chromatography followed by gel filtration. high crp-binding activity was found in a fraction of mol. wt c. 500,000 which was characterised by strong binding to the hydrophobic column. three fractions of less conspicuous crp-binding activity were identified (c. 500 000, 150 000 and 150 000-50 00 ...	1986	3512843
rapid visualization of three common fungi using fluorescein-conjugated lectins.	the feasibility of using fluorescein-conjugated lectins to visualize and differentiate three fungi commonly involved in ophthalmic mycoses was evaluated. using a panel of fluorescein-conjugated lectins, candida albicans, aspergillus fumigatus, and fusarium solani were rapidly and reproducibly visualized in in vitro culture isolates, as well as in tissue samples and fixed histopathologic specimens taken from experimental mycoses. additionally, aspergillus and fusarium were consistently differenti ...	1986	3514531
histopathologic and antibody responses of rabbits exposed to aerosols containing spores of aspergillus fumigatus: comparison of single and multiple exposures.	resistance to pulmonary aspergillosis was studied in groups of rabbits exposed to aerosolized spores of aspergillus fumigatus for 15 minutes on successive days for a total of 10, 7, or 4 exposures or a single exposure. the results of the study demonstrated that exposure of rabbits to spores for 15 minutes on 10 successive days did not result in an accumulation of viable spores in excess of those present in the lungs of rabbits exposed a single time. the tissue response in the lungs of the rabbit ...	1986	3516034
allergic bronchopulmonary aspergillosis. natural history and classification of early disease by serologic and roentgenographic studies.	eighty-four patients with allergic bronchopulmonary aspergillosis (abpa) were evaluated for a total of 294 patient-years with a mean observation period of 3.7 years and classified by the stage of abpa. the largest percentage of patients were in the stage iv (corticosteroid-dependent asthma stage) group. the next largest percentage were in the stage v (fibrotic, end-stage lung disease) group. of the latter 24 patients, eight had died. in addition, we describe 13 patients with all serologic charac ...	1986	3516103
osteomyelitis and arthritis associated with aspergillus fumigatus in a dog.		1986	3516132
[inactivation of the fungus aspergillus fumigatus].		1986	3516632
serum fungistatic and fungicidal activity in volunteers receiving antifungal agents.	the serum levels of two imidazoles, ketoconazole and bayer n7133, when administered alone or in combination with rifampin, were measured in volunteers and the serum antifungal activity was determined against various fungal strains. serum levels were measured by high pressure liquid chromatography and correlated with serum fungistatic activity as determined in microtiter plates. ketoconazole showed a wide range of serum levels, suggesting individual variations. the addition of two doses of rifamp ...	1986	3516681
inhibition of cellular immunity by products of aspergillus fumigatus.	a fraction, fb, from extracts of aspergillus fumigatus which previously was found to display a high degree of cellular hypersensitivity in skin tests in sensitized guinea-pigs but a low ability to affect transformation of lymphocytes in vitro, was studied. this fraction was found to be capable of inhibiting lymphocyte transformation induced in vitro by tuberculin and by t and b cell mitogens. the inhibitory properties of fb on lymphocyte activation were heat labile, destroyed by proteolysis, and ...	1986	3517279
pulmonary aspergilloma. case reports from malaysia.	pulmonary aspergilloma is by no means uncommon in malaysia. the lack of documentation of its occurrence in malaysia, is mainly due to the lack of clinical awareness, and the absence of facilities for the proper diagnosis of the infection.	1986	3520330
[a case of cerebral aspergilloma following radical operation of a cerebral aneurysm].	a case of aspergillotic abscess with granuloma is reported. a 45-year-old man was admitted to our hospital on apr. 10, 1984 due to the rupture of an aneurysm of the anterior communicating artery. neck clipping of the aneurysm was proposed on apr. 12, 1984, but was not performed because of cardiac arrest with unknown etiology during the operation. neck clipping was performed on apr. 23, 1984. antibiotic therapy was prolonged for about three weeks. about 6 months after surgery, he was readmitted o ...	1986	3520367
[pulmonary aspergillosis in a child with septic granulomatosis].		1986	3520537
[limited aspergillosis of the bronchi].		1986	3520544
[the dot immunobinding assay for specific ige and igg antibodies to dermatophagoides farinae].		1986	3521548
class-specific antibody determination against aspergillus fumigatus by means of the enzyme-linked immunosorbent assay. iii. comparative study: igg, iga, igm elisa titers, precipitating antibodies and ige binding after fractionation of the antigen.	igg, iga and igm elisa antibody titers against aspergillus fumigatus were elevated in sera of patients with aspergilloma and allergic bronchopulmonary aspergillosis (abpa), showing higher titers for the igg antibodies compared with the iga and igm antibodies. no differences were found between titers of identical antibody classes in the two groups of sera. igg and iga elisa titers were highly specific whereas igm elisa showed more unspecific binding of igm antibodies. antibodies, as measured by e ...	1986	3522437