Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| serine proteases sp1 and sp13 mediate the melanization response of asian corn borer, ostrinia furnacalis, against entomopathogenic fungus beauveria bassiana. | exposure to entomopathogenic fungi is one approach for insect pest control. little is known about the immune interactions between fungus and its insect host. melanization is a prominent immune response in insects in defending against pathogens such as bacteria and fungi. clip domain serine proteases in insect plasma have been implicated in the activation of prophenoloxidase, a key enzyme in the melanization. the relationship between host melanization and the infection by a fungus needs to be est ... | 2015 | 25900291 |
| assessing gene expression during pathogenesis: use of qrt-pcr to follow toxin production in the entomopathogenic fungus beauveria bassiana during infection and immune response of the insect host triatoma infestans. | entomopathogenic fungi secrete toxic secondary metabolites during the invasion of the insect hemocoel as part of the infection process. although these compounds have been frequently mentioned as virulence factors, the roles of many of them remain poorly understood, including the question of whether they are expressed during the infection process. a major hurdle to this issue remains the low sensitivity of biochemical detection techniques (e.g., hplc) within the complex samples that may contain t ... | 2015 | 25912088 |
| habitat selection of a parasitoid mediated by volatiles informing on host and intraguild predator densities. | to locate and evaluate host patches before oviposition, parasitoids of herbivorous insects utilize plant volatiles and host-derived cues, but also evaluate predator-derived infochemicals to reduce predation risks. when foraging in host habitats infested with entomopathogenic fungi that can infect both a parasitoid and its host, parasitoids may reduce the risk of intraguild predation (igp) by avoiding such patches. in this study, we examined whether the presence of the entomopathogenic fungi meta ... | 2015 | 25943193 |
| effect of biochars and microorganisms on cadmium accumulation in rice grains grown in cd-contaminated soil. | cadmium (cd) contaminated in rice grains is a serious problem because most asians consume rice on a daily basis. rice grown in cd-contaminated soil normally did not have high concentration of cd. however, soil samples used in this study had high concentrations of cd. the purpose of this study was to clearly see the effects of biochar and microorganism addition in rice growing in cd-contaminated soil. the initial cd concentration in cd-contaminated soil used in this study was about 650 mg kg(-1). ... | 2016 | 25943511 |
| glucose concentration alters dissolved oxygen levels in liquid cultures of beauveria bassiana and affects formation and bioefficacy of blastospores. | the filamentous fungus beauveria bassiana is an economically important pathogen of numerous arthropod pests and is able to grow in submerged culture as filaments (mycelia) or as budding yeast-like blastospores. in this study, we evaluated the effect of dissolved oxygen and high glucose concentrations on blastospore production by submerged cultures of two isolates of b. bassiana, esalq1432 and gha. results showed that maintaining adequate dissolved oxygen levels coupled with high glucose concentr ... | 2015 | 25947245 |
| transcriptional control of fungal cell cycle and cellular events by fkh2, a forkhead transcription factor in an insect pathogen. | transcriptional control of the cell cycle by forkhead (fkh) transcription factors is likely associated with fungal adaptation to host and environment. here we show that fkh2, an ortholog of yeast fkh1/2, orchestrates cell cycle and many cellular events of beauveria bassiana, a filamentous fungal insect pathogen. deletion of fkh2 in b. bassiana resulted in dramatic down-regulation of the cyclin-b gene cluster and hence altered cell cycle (longer g2/m and s, but shorter g0/g1, phases) in unicellul ... | 2015 | 25955538 |
| soil application of beauveria bassiana gha against apple sawfly, hoplocampa testudinea (hymenoptera: tenthredinidae): field mortality and fungal persistence. | low impact alternatives to synthetic insecticides for the control of apple sawfly (hoplocampa testudinea klug) are scarce encumbering pest management in organic apple orchards. we investigated the soil persistence and field efficacy of the entomopathogenic fungus beauveria bassiana (balsamo) vuillemin (botanigard) against apple sawfly under common organic orchard practices. we also assessed the efficacy of b. bassiana gha and metarhizium brunneum petch (indigenous strain) against sawfly in the l ... | 2016 | 25959579 |
| biological control of the chagas disease vector triatoma infestans with the entomopathogenic fungus beauveria bassiana combined with an aggregation cue: field, laboratory and mathematical modeling assessment. | current chagas disease vector control strategies, based on chemical insecticide spraying, are growingly threatened by the emergence of pyrethroid-resistant triatoma infestans populations in the gran chaco region of south america. | 2015 | 25969989 |
| regulation of a phenylalanine ammonia lyase (bbpal) by calmodulin in response to environmental changes in the entomopathogenic fungus beauveria bassiana. | phenylalanine ammonia lyase (pal, e.c. 4.3.1.5) catalyses the deamination of l -phenylalanine to trans-cinnamic acid and ammonia, facilitating a critical step in the phenylpropanoid pathway that produces a variety of secondary metabolites. in this study, we isolated bbpal gene in the entomopathogenic fungus beauveria bassiana. according to multiple sequence alignment, homology modelling and in vitro pal activity, we demonstrated that bbpal acts as a typical pal enzyme in b. bassiana. bbpal inter ... | 2015 | 25970691 |
| phylogeny of type i polyketide synthases (pkss) in fungal entomopathogens and expression analysis of pks genes in beauveria bassiana bcc 2660. | entomopathogenic fungi are able to invade and kill insects. various secondary metabolites can mediate the interaction of a fungal pathogen with an insect host and also help the fungus compete with other microbes. here we screened 23 isolates of entomopathogenic fungi for polyketide synthase (pks) genes and amplified 72 pks gene fragments using degenerate pcr. we performed a phylogenetic analysis of conserved ketosynthase and acyltransferase regions in these 72 sequences and 72 pkss identified fr ... | 2015 | 25986551 |
| high throughput profiling of the cotton bollworm helicoverpa armigera immunotranscriptome during the fungal and bacterial infections. | innate immunity is essential in defending against invading pathogens in invertebrates. the cotton bollworm, helicoverpa armigera (hübner) is one of the most destructive lepidopteran pests, which causes enormous economic losses in agricultural production worldwide. the components of the immune system are largely unknown in this insect. the application of entomopathogens is considered as an alternative to the chemical insecticides for its control. however, few studies have focused on the molecular ... | 2015 | 26001831 |
| expression profiles of astakine-like transcripts in the tarnished plant bug, lygus lineolaris, exposed to fungal spores of beauveria bassiana. | astakines are hematopoietic cytokines originally isolated from crustaceans. we identified three astakine-like transcripts in the tarnished plant bug (lygus lineolaris), llast-1, llast-2 and llast-3, containing prokineticin domains. quantitative real-time pcr showed variation in expression patterns of astakines in different tissues and between sexes. relative expression levels of llast-1 were highest in the fat bodies of females, while llast-2 expression was highest in the fat bodies of both male ... | 2015 | 26018035 |
| [natural ocurrence of entomopathogenic fungi in soils cultivated with paraguay tea (ilex paraguariensis st. hil.) in misiones, argentina]. | this study aimed to morphologically isolate, identify and characterize entomopathogenic fungi present in soils cultivated with paraguay tea (ilex paraguariensis). a survey of native entomopathogenic fungi was conducted from 40 soil samples grown with paraguay tea in the province of misiones, argentina, from may 2008 to june 2010. the soil dilution plate methodology on selective culture media was used to isolate microorganisms. taxonomic identification was performed using macroscopic and microsco ... | 2015 | 26028585 |
| intra- and trans-generational effects of larval diet on susceptibility to an entomopathogenic fungus, beauveria bassiana, in the greater wax moth, galleria mellonella. | in addition to nutritional conditions experienced by individuals themselves, those experienced by their parents can affect their immune function. here, we studied the intra- and trans-generational effects of larval diet on susceptibility to an entomopathogenic fungus, beauveria bassiana, in the greater wax moth, galleria mellonella. in the first part of the study, a split-brood design was used to compare the susceptibility of full sibs raised either on low- or on high-nutrition larval diet. in t ... | 2015 | 26052853 |
| tenebrionid secretions and a fungal benzoquinone oxidoreductase form competing components of an arms race between a host and pathogen. | entomopathogenic fungi and their insect hosts represent a model system for examining invertebrate-pathogen coevolutionary selection processes. here we report the characterization of competing components of an arms race consisting of insect protective antimicrobial compounds and evolving fungal mechanisms of detoxification. the insect pathogenic fungus beauveria bassiana has a remarkably wide host range; however, some insects are resistant to fungal infection. among resistant insects is the teneb ... | 2015 | 26056261 |
| a critical role for clsp2 in the modulation of antifungal immune response in mosquitoes. | entomopathogenic fungi represent a promising class of bio-insecticides for mosquito control. thus, detailed knowledge of the molecular mechanisms governing anti-fungal immune response in mosquitoes is essential. in this study, we show that clsp2 is a modulator of immune responses during anti-fungal infection in the mosquito aedes aegypti. with a fungal infection, the expression of the clsp2 gene is elevated. clsp2 is cleaved upon challenge with beauveria bassiana conidia, and the liberated clsp2 ... | 2015 | 26057557 |
| addendum to: molecular characterization of a novel amalgavirus from the entomopathogenic fungus beauveria bassiana. | 2015 | 26071246 | |
| diversity of entomopathogenic hypocreales in soil and phylloplanes of five mediterranean cropping systems. | the diversity of entomopathogenic hypocreales from the soil and phylloplanes in five mediterranean cropping systems with different degrees of management [organic olive orchard conventional olive orchard, holm oak reforestation, holm oak dehesa (a multifunctional agro-sylvo-pastoral system), and sunflower plantation] was studied during four seasons. a total of 697 entomopathogenic fungal isolates were obtained from 272 soil samples, 1608 crop phylloplane samples and 1368 weed phylloplane samples. ... | 2015 | 26146223 |
| short-term heat shock affects the course of immune response in galleria mellonella naturally infected with the entomopathogenic fungus beauveria bassiana. | we aimed to investigate how exposition of infected insects to short-term heat shock affects the biochemical and molecular aspects of their immune response. galleria mellonella larvae were exposed to 43°c for 15min, at the seventy second hour after natural infection with entomopathogenic fungus beauveria bassiana. as a result, both qualitative and quantitative changes in hemolymph protein profiles, and among them infection-induced changes in the amount of apolipophorin iii (apolp-iii), were obser ... | 2015 | 26149823 |
| feeding on beauveria bassiana-treated frankliniella occidentalis causes negative effects on the predatory mite neoseiulus barkeri. | the entomopathogenic fungus beauveria bassiana and the predatory mite neoseiulus barkeri are both potential biocontrol agents for their shared host/prey frankliniella occidentalis. the combination of the two agents may enhance biological control of f. occidentalis if the fungus does not negatively affect n. barkeri. this study evaluated the indirect effects of b. bassiana strain sz-26 on n. barkeri mediated by f. occidentalis using the age-stage, two-sex life table. when fed on the first instar ... | 2015 | 26153532 |
| survival rate and transcriptional response upon infection with the generalist parasite beauveria bassiana in a world-wide sample of drosophila melanogaster. | the ability to cope with infection by a parasite is one of the major challenges for any host species and is a major driver of evolution. parasite pressure differs between habitats. it is thought to be higher in tropical regions compared to temporal ones. we infected drosophila melanogaster from two tropical (malaysia and zimbabwe) and two temperate populations (the netherlands and north carolina) with the generalist entomopathogenic fungus beauveria bassiana to examine if adaptation to local par ... | 2015 | 26154519 |
| a novel ras gtpase (ras3) regulates conidiation, multi-stress tolerance and virulence by acting upstream of hog1 signaling pathway in beauveria bassiana. | two ras atpases (ras1 and ras2) are well known to regulate antagonistically or cooperatively various cellular events in many fungi. here we show the significance of a novel ras homolog (ras3) for beauveria bassiana. ras3 possesses five domains and two gtp/gdp switches typical for ras family and was proven to localize to plasma membrane despite the position change of a membrane-targeting cysteine in c-terminal caax motif. deletion of ras3 altered temporal transcription pattern of ras1 instead of ... | 2015 | 26162967 |
| modulation of conidia production and expression of the gene bbrgs1 from beauveria bassiana by oxygen pulses and light. | light and oxidant states affect the conidiation in diverse fungi, although the response has not been described when both stimuli are applied simultaneously. conidial production and quality in beauveria bassiana were analysed under four conditions for a wild-type (wt) strain and a previously isolated mutant (mt): normal atmosphere (21% o2; na) or oxygen-enriched pulses (26% o2; oep), with either light (l) or darkness (d). the response was complemented by following the expression of the bbrgs1 gen ... | 2015 | 26166809 |
| dopamine modulates hemocyte phagocytosis via a d1-like receptor in the rice stem borer, chilo suppressalis. | dopamine (da) is a signal moiety bridging the nervous and immune systems. da dysregulation is linked to serious human diseases, including addiction, schizophrenia, and parkinson's disease. however, da actions in the immune system remain incompletely understood. in this study, we found that da modulates insect hemocyte phagocytosis using hemocytes prepared from the rice stem borer (rsb), chilo suppressalis. we investigated whether insect hemocytes are capable of de novo da production. here we sho ... | 2015 | 26179416 |
| laboratory evaluation of a native strain of beauveria bassiana for controlling dermanyssus gallinae (de geer, 1778) (acari: dermanyssidae). | the poultry red mite, dermanyssus gallinae (de geer, 1778) (acari: dermanyssidae) is one of the most economically important ectoparasites of laying hens worldwide. chemical control of this mite may result in environmental and food contamination, as well as the development of drug resistance. high virulence of beauveria bassiana sensu lato strains isolated from naturally infected hosts or from their environment has been demonstrated toward many arthropod species, including ticks. however, a limit ... | 2015 | 26206607 |
| effect of surfactants and temperature on germination and vegetative growth of beauveria bassiana. | three non-ionic surfactants: tween20, tween80 and breakthru (®) were screened for their effects on spore germination and mycelial growth rates and for their influence on three isolates of beauveria bassiana spore germination at various temperatures. tween20 and tween80 were compatible with all the b. bassiana isolates in the germination studies, but inhibited germination at higher surfactant concentrations, irrespective of the conidial concentrations . breakthru (®) had an inhibitory effect on g ... | 2015 | 26221090 |
| the effect of beauveria bassiana infection on cell mediated and humoral immune response in house fly, musca domestica l. | entomopathogenic fungi that manifest infections by overcoming insect's immune response could be a successful control agent for the house fly, musca domestica l. which is a major domestic, medical, and veterinary pest. in this study, the immune response of house fly to beauveria bassiana infection was investigated to reveal fundamental aspects of house fly hemocyte biology, such as hemocyte numbers and size, which is poorly understood. the total hemocyte counts (thcs) in b. bassiana-infected hous ... | 2015 | 26233748 |
| eleven microbial metabolites of 6-hydroxyflavanone. | 6-hydroxyflavanone (1) when fermented with fungal culture cunninghamella blakesleeana (atcc 8688a) yielded flavanone 6-o-β-d-glucopyranoside (2), flavanone 6-sulfate (3), and 6-hydroxyflavanone 7-sulfate (4). aspergillus alliaceus (atcc 10060) also transformed 1 to metabolite 3 as well as 4'-hydroxyflavanone 6-sulfate (5) and 6,4'-dihydroxyflavanone (6). beauveria bassiana (atcc 7159) metabolized 1 to 6 and flavanone 6-o-β-d-4-o-methyglucopyranoside (7). mucor ramannianus (atcc 9628) transformed ... | 2015 | 26235165 |
| involvement of a caleosin in lipid storage, spore dispersal, and virulence in the entomopathogenic filamentous fungus, beauveria bassiana. | eukaryotic cells store lipids in membrane-encased droplets. the entomopathogenic fungus, beauveria bassiana, initiates infection via attachment of its spores to the epicuticle or waxy layer of target insects, degrading and assimilating host surface hydrocarbons, carbohydrates and proteins. caleosins are components of the proteinaceous coat of lipid droplets and a single b. bassiana caleosin homologue, bbcal1, was identified and characterized. the bbcal1 sequence contained an ef-hand ca(2+) bindi ... | 2015 | 26235819 |
| weta and vosa are distinct regulators of conidiation capacity, conidial quality, and biological control potential of a fungal insect pathogen. | many filamentous fungi produce only conidia for dispersal and survival in vitro or in vivo. here, we show that the developmental regulator weta and the velvet protein vosa are not only required for conidial maturation but indispensable for conidiation in beauveria bassiana, a filamentous entomopathogen. deletion of weta or vosa resulted in more than 90 % transcriptional depression of brla and abaa, two activator genes in the central developmental pathway, during the critical period of conidiopho ... | 2015 | 26243054 |
| selectivity of organic products to trichogramma pretiosum riley (hymenoptera: trichogrammatidae). | the selectivity of various entomopathogens and one insecticide (chlorpyrifos = positive control) to trichogramma pretiosum riley (hymenoptera: trichogrammatidae) was evaluated in the laboratory, using the protocol established by the working group on "pesticides and beneficial organisms" of the iobc. the evaluated parameters were parasitism (%), adult emergence (%), and product repellency to the parasitoid when sprayed on host eggs prior to parasitism (free-choice and no-choice tests). most of th ... | 2015 | 26267248 |
| fungal biosynthesis of the bibenzoquinone oosporein to evade insect immunity. | quinones are widely distributed in nature and exhibit diverse biological or pharmacological activities; however, their biosynthetic machineries are largely unknown. the bibenzoquinone oosporein was first identified from the ascomycete insect pathogen beauveria bassiana>50 y ago. the toxin can also be produced by different plant pathogenic and endophytic fungi with an array of biological activities. here, we report the oosporein biosynthetic machinery in fungi, a polyketide synthase (pks) pathway ... | 2015 | 26305932 |
| interplay between calcineurin and the slt2 map-kinase in mediating cell wall integrity, conidiation and virulence in the insect fungal pathogen beauveria bassiana. | the entomopathogenic fungus, beauveria bassiana, is of environmental and economic importance as an insect pathogen, currently used for the biological control of a number of pests. cell wall integrity and conidiation are critical parameters for the ability of the fungus to infect insects and for production of the infectious propagules. the contribution of calcineurin and the slt2 map kinase to cell wall integrity and development in b. bassiana was investigated. gene knockouts of either the calcin ... | 2015 | 26319315 |
| the fungistatic and fungicidal effects of volatiles from metathoracic glands of soybean-attacking stink bugs (heteroptera: pentatomidae) on the entomopathogen beauveria bassiana. | this study was initially designed to evaluate the differential susceptibility of three soybean-attacking pentatomids to the entomopathogenic fungus beauveria bassiana in standardized bioassays. euschistus heros (eh) was shown to be significantly less susceptible than chinavia ubica (cu), whereas dichelops melacanthus (dm) adults were highly susceptible to fungal infections. a deeper look at the mechanisms involved in the possible role of volatiles from metathoracic glands on fungal infections wa ... | 2015 | 26321153 |
| a protocol for determination of conidial viability of the fungal entomopathogens beauveria bassiana and metarhizium anisopliae from commercial products. | techniques for directly determining conidial viability have widespread use but also have limitations for quality control assessments of formulated mycoinsecticides, especially in emulsifiable oil. this study proposes a new method based on adaptations of already established protocols that use the direct viability method to make it more economical and accurate, thus enabling its use in the evaluation of formulated products. appropriate parameters and conditions were defined using products based on ... | 2015 | 26432104 |
| antimicrobial activity of stomoxys calcitrans against beauveria bassiana sensu lato isolates. | this study had the aims of evaluating the antimicrobial characteristics of stomoxys calcitrans (diptera: muscidae) larvae against the fungal isolates cg138, cg228 and esalq986 of beauveria bassiana sensu lato (balsamo-crivelli) vuillemin, 1912 (hypocreales: cordycipitaceae). s. calcitrans eggs, larvae and pupae were exposed to these same isolates. statistical analysis showed that the immature stages of s. calcitrans were not susceptible to the fungal isolates used, regardless of the exposure met ... | 2015 | 26444064 |
| occurrence and prevalence of insect pathogens in populations of the codling moth, cydia pomonella l.: a long-term diagnostic survey. | about 20,550 larvae, pupae and adults of the codling moth, cydia pomonella l., were diagnosed for pathogens during long-term investigations (1955-2012) at the institute for biological control in darmstadt, germany. the prevailing entomopathogens diagnosed in these studies were insect pathogenic fungi, especially beauveria bassiana and isaria farinosa, the microsporidium, nosema carpocapsae, the cydia pomonella granulovirus (cpgv), as well as mostly undetermined bacteria. while the cpgv was obser ... | 2013 | 26462428 |
| entomopathogenic fungi associated with exotic invasive insect pests in northeastern forests of the usa. | mycopathogens of economically important exotic invasive insects in forests of northeastern usa have been the subject of research at the entomology research laboratory, university of vermont, for the last 20 years. elongate hemlock scale, european fruit lecanium, hemlock woolly adelgid and pear thrips were analyzed for the presence of mycopathogens, in order to consider the potential for managing these pests with biological control. fungal cultures isolated from insects with signs of fungal infec ... | 2013 | 26462527 |
| tri-tek (petroleum horticultural oil) and beauveria bassiana: use in eradication strategies for bemisia tabaci mediterranean species in uk glasshouses. | the sweetpotato whitefly bemisia tabaci (gennadius) (hemiptera: aleyrodidae) is a pest of global importance on both outdoor and glasshouse crops. to date, b. tabaci has not become established in the uk. the uk holds protected zone status against this pest and, as a result, b. tabaci entering on plant material is subjected to a policy of eradication. mediterranean species is now the most prevalent bemisia species entering the uk. increasing neonicotinoid resistance is becoming increasingly widesp ... | 2015 | 26463071 |
| virulence of botanigard(®) to second instar brown marmorated stink bug, halyomorpha halys (stål) (heteroptera: pentatomidae). | the brown marmorated stink bug, halyomorpha halys (stål) (bmsb) is an exotic invasive insect originating in east asia, currently causing significant damage to fruits, vegetables and other crops throughout most of the mid-atlantic states of the u.s. it also is a nuisance pest, entering homes in the fall in search of suitable overwintering sites. two formulations of botanigard(®) with a strain of beauveria bassiana (gha) as the active ingredient were tested against second instar bmsb. both the wet ... | 2015 | 26463187 |
| update on the status of bemisia tabaci in the uk and the use of entomopathogenic fungi within eradication programmes. | the sweetpotato whitefly bemisia tabaci (gennadius) (hemiptera: aleyrodidae) continues to be a serious threat to crops worldwide. the uk holds protected zone status against this pest and, as a result, b. tabaci entering on plant material is subjected to a policy of eradication. both b and q bemisia biotypes are now regularly intercepted entering the uk. with increasing reports of neonicotinoid resistance in both these biotypes, it is becoming more problematic to control/eradicate. therefore, alt ... | 2013 | 26464385 |
| interaction between tata-binding protein (tbp) and multiprotein bridging factor-1 (mbf1) from the filamentous insect pathogenic fungus beauveria bassiana. | tata-binding protein (tbp) is a ubiquitous component of eukaryotic transcription factors that acts to nucleate assembly and position pre-initiation complexes. multiprotein bridging factor 1 (mbf1) is thought to interconnect tbp with gene specific transcriptional activators, modulating transcriptional networks in response to specific signal and developmental programs. the insect pathogen, beauveria bassiana, is a cosmopolitan fungus found in most ecosystems where it acts as an important regulator ... | 2015 | 26466369 |
| integrated management of european cherry fruit fly rhagoletis cerasi (l.): situation in switzerland and europe. | the european cherry fruit fly, rhagoletis cerasi (l.) (diptera: tephritidae), is a highly destructive pest. the low tolerance for damaged fruit requires preventive insecticide treatments for a marketable crop. the phase-out of old insecticides threatens cherry production throughout the european union (eu). consequently, new management techniques and tools are needed. with the increasing number of dwarf tree orchards covered against rain to avoid fruit splitting, crop netting has become a viable, ... | 2012 | 26466721 |
| antimicrobial properties of nest volatiles in red imported fire ants, solenopsis invicta (hymenoptera: formicidae). | the antimicrobial property of volatiles produced by red imported fire ants, solenopsis invicta, against beauveria bassiana, a common entomopathogenic fungus, was demonstrated. the germination rate of b. bassiana spores was significantly reduced after they were exposed to volatiles within an artificial ant nest. since the air that contained the same level of o2 and co2 as that in artificial fire ant nests did not suppress the germination rate of b. bassiana, the observed reduction of germination ... | 2015 | 26467352 |
| susceptibility of cabbage maggot larvae (diptera: anthomyiidae) to hypocreales entomopathogenic fungi. | the pathogenicity of six metarhizium spp., four beauveria bassiana (balsamo) vuillemin, and four tolypocladium cylindrosporum gams (ascomycota: hypocreales) fungal pathogens exposed to third-instar delia radicum l. was evaluated in laboratory bioassays. the presence of intra- and intergeneric variations concerning the pathogenicity of the isolates was investigated. results show that all metarhizium spp. and t. cylindrosporum isolates caused a noteworthy mortality to the third instar and conseque ... | 2015 | 26470101 |
| new approach of beauveria bassiana to control the red palm weevil (coleoptera: curculionidae) by trapping technique. | this work is the first study to investigate the efficacy of the commercial formulation of beauveria bassiana (broadband) to control adults of red palm weevil (rhynchophorus ferrugineus (olivier)). this fungus could be applied as one of the biological tactics in controlling red palm weevil. bioassay experiments for medium lethal concentrate and medium time to cause death of 50% of red palm weevil adults were carried out. the result showed that the lc50 of b. bassiana (broadband) was 2.19×10(7) an ... | 2015 | 26470153 |
| acoustic assessment of beauveria bassiana (hypocreales: clavicipitaceae) effects on rhynchophorus ferrugineus (coleoptera: dryophthoridae) larval activity and mortality. | rhynchophorus ferrugineus (olivier) (coleoptera: dryophthoridae) is an economically important pest of palm trees in the subtropics. beauveria bassiana (balsamo-crivelli) vuillemin (hypocreales: clavicipitaceae), has been shown to be pathogenic against r. ferrugineus in laboratory and field studies. however, because they remain inside the trunks until adulthood, the slowing of feeding and increases in mortality of internally feeding r. ferrugineus larvae over time after b. bassiana treatment has ... | 2015 | 26470155 |
| sublethal effects of beauveria bassiana (ascomycota: hypocreales) on life table parameters of frankliniella occidentalis (thysanoptera: thripidae). | we assessed effects of parental exposure to beauveria bassiana on life history traits of subsequent generations of western flower thrips, frankliniella occidentalis (pergande) (thysanoptera: thripidae). progeny from individuals that survived fungal exposure as second instars had significantly shorter egg stages, but longer prepupal development times than corresponding untreated controls. however, survivorship to adulthood of these progeny groups did not differ. although fecundities of the parent ... | 2015 | 26470219 |
| horizontal transmission of beauveria bassiana (hypocreales: cordycipitaceae) and metarhizium anisopliae (hypocreales: clavicipitaceae) in musca domestica (diptera: muscidae). | beauveria bassiana vuillemin and metarhizium anisopliae (metschnikoff) sorokin are fungi with potential for controlling musca domestica l. however, the impact on this dipteral may vary depending on the fungal isolates and the methodology used. this study evaluated the pathogenicity of direct application and horizontal transmission of b. bassiana (cg240) and m. anisopliae (cg34) on adult m. domestica individuals. the impact of b. bassiana and m. anisopliae on m. domestica was evaluated at the con ... | 2015 | 26470298 |
| insecticide rotation programs with entomopathogenic organisms for suppression of western flower thrips (thysanoptera: thripidae) adult populations under greenhouse conditions. | western flower thrips, frankliniella occidentalis (pergande), is one of the most destructive insect pests of greenhouse production systems with the ability to develop resistance to a wide variety of insecticides. a common resistance management strategy is rotating insecticides with different modes of action. by incorporating entomopathogenic organisms (fungi and bacteria), which have discrete modes of action compared to standard insecticides, greenhouse producers may preserve the effectiveness o ... | 2015 | 26470338 |
| antagonism of entomopathogenic fungi by bacillus spp. associated with the integument of cicadellids and delphacids. | entomopathogenic fungi are potential tools to biocontrol cicadellids and delphacids, two groups of insects that cause extensive damage to agricultural crops. however, bacteria living on the host cuticle may inhibit fungal growth. in the present work, following the molecular characterization of 10 strains of bacillus isolated from the integument of cicadellids and delphacids, we selected isolates of the fungi beauveria bassiana and metarhizium anisopliae that are resistant to the antimicrobials s ... | 2015 | 26496616 |
| cloning and expression analysis of the chitinase gene ifu-chit2 from isaria fumosorosea. | entomopathogenic fungi can produce a series of chitinases, some of which function synergistically with proteases and other hydrolytic enzymes to degrade the insect cuticle. in the present study, the chitinase gene ifu-chit2 from isaria fumosorosea was investigated. the ifu-chit2 gene is 1,435-bp long, interrupted by three short introns, and encodes a predicted protein of 423 amino acids with a 22 residue signal peptide. the predicted ifu-chit2 protein is highly homologous to beauveria bassiana c ... | 2017 | 26500443 |
| beauveria bassiana lipase a expressed in komagataella (pichia) pastoris with potential for biodiesel catalysis. | lipases (ec 3.1.1.3) comprise a biotechnologically important group of enzymes because they are able to catalyze both hydrolysis and synthesis reactions, depending on the amount of water in the system. one of the most interesting applications of lipase is in the biofuel industry for biodiesel production by oil and ethanol (or methanol) transesterification. entomopathogenic fungi, which are potential source of lipases, are still poorly explored in biotechnological processes. the present work repor ... | 2015 | 26500628 |
| insights from the genome of ophiocordyceps polyrhachis-furcata to pathogenicity and host specificity in insect fungi. | ophiocordyceps unilateralis is an outstanding insect fungus for its biology to manipulate host ants' behavior and for its extreme host-specificity. through the sequencing and annotation of ophiocordyceps polyrhachis-furcata, a species in the o. unilateralis species complex specific to the ant polyrhachis furcata, comparative analyses on genes involved in pathogenicity and virulence between this fungus and other fungi were undertaken in order to gain insights into its biology and the emergence of ... | 2015 | 26511477 |
| prophenoloxidase genes and antimicrobial host defense of the model beetle, tribolium castaneum. | in this study, we characterized prophenoloxidase (propo, (ppo)) genes of tribolium castaneum and examined their involvement in antimicrobial host defense. amino acid sequence comparison with well-characterized ppo proteins from other insect species suggested that t. castaneum ppo genes encoded functional proenzymes, with crucial sequence motifs being conserved. developmental kinetics of the mrna of two ppo genes, ppo1 and ppo2 in the pupal stage were different to each other. the ppo1 mrna levels ... | 2015 | 26519623 |
| efficacy of sub lethal concentration of entomopathogenic fungi on the feeding and reproduction of spodoptera litura. | in the present investigation, impact of sub lethal concentrations of entomopathogenic fungi, namely isaria fumosorosea, beauveria bassiana and paecilomyces variotii, secondary metabolite on feeding, growth, fecundity and hatchability of spodoptera litura was performed. the s. litura treated with i. fumosorosea and b. bassiana metabolites exhibited renounced food consumption. the growth rate of treated s. litura with metabolite of i. fumosorosea had drastic reduction. in the case of approximate d ... | 2015 | 26558183 |
| growth and metabolism of beauveria bassiana spores and mycelia. | fungi are ubiquitous in nature and have evolved over time to colonize a wide range of ecosystems including pest control. to date, most research has focused on the hypocrealean genera beauveria bassiana, which is a typical filamentous fungus with a high potential for insect control. the morphology and components of fungi are important during the spores germination and outgrow to mycelia. however, to the best of our knowledge, there is no report on the morphology and components of b. bassiana spor ... | 2015 | 26581712 |
| identification and characterization of an insect toxin protein, bb70p, from the entomopathogenic fungus, beauveria bassiana, using galleria mellonella as a model system. | an insect-toxic protein, bb70p, was purified from beauveria bassiana 70 using ammonium sulfate precipitation, ion exchange chromatography, and gel filtration. bb70p has a high affinity for anion exchangers and 2d electrophoresis results revealed a single spot with a molecular weight of 35.5 kda and an iso-electric point of ∼4.5. bb70p remains active from 4 to 60°c, within a ph range of 4-10, but is more active in slightly acidic ph. a pure protein, bb70p does not have any carbohydrate side chain ... | 2016 | 26592942 |
| successful treatment of beauveria bassiana fungal keratitis with topical voriconazole. | we describe a 66-year-old woman who suffered from fungal keratitis after corneal transplantation. the causative organism was identified as beauveria bassiana on the basis of morphological characteristics and the sequence of the internal transcribed spacer region of the ribosomal rna gene. the patient was successfully treated with topical voriconazole (vrcz) use only. we, hereby, present the first report of a case with b. bassiana fungal keratitis that responded to topical antifungal vrcz treatme ... | 2016 | 26603426 |
| a distinctive pattern of beauveria bassiana-biotransformed ginsenoside products triggers mitochondria/fasl-mediated apoptosis in colon cancer cells. | ginseng is one of the most commonly used adaptogens. transformation into the minor ginsenosides produces compounds with more effective action. beauveria bassiana, a teleomorph of cordyceps bassiana, is a highly efficient producer of mammalian steroids and produces large amounts of sugar-utilizing enzymes. however, the fermentation of steroid glycosides in ginseng with b. bassiana has never been studied. thus, we evaluated the bioconversion of the major ginsenosides in white ginseng by b. bassian ... | 2016 | 26609787 |
| immune and metabolic responses of chilo suppressalis walker (lepidoptera: crambidae) larvae to an insect growth regulator, hexaflumuron. | efficient control of chilo suppressalis walker is always controversial due to highly economic damage, resistance to insecticides and environmental pollutions. so, combination of safe pest controls e.g. biocontrol agents and insect growth regulators seems to be promising via integrated pest management program. bioassay of hexaflumuron on 4th larval instars revealed concentrations of 44.34, 179.74 and 474.94µg/ml as lc10-50 values. numbers of total hemocytes, plasmatocytes and granulocytes as well ... | 2015 | 26615153 |
| genes involved in virulence of the entomopathogenic fungus beauveria bassiana. | pest insects cause severe damage to global crop production and pose a threat to human health by transmitting diseases. traditionally, chemical pesticides (insecticides) have been used to control such pests and have proven to be effective only for a limited amount of time because of the rapid spread of genetic insecticide resistance. the basis of this resistance is mostly caused by (co)dominant mutations in single genes, which explains why insecticide use alone is an unsustainable solution. there ... | 2016 | 26628209 |
| effect of temperature and humidity on pathogenicity of native beauveria bassiana isolate against musca domestica l. | beauveria bassiana hq917687 virulence to housefly larvae and adult was assessed at different relative humidity, rh (50, 75, 90, and 100 %) and temperature (15, 20, 25, 30, 35, 40, 45 °c) conditions at the fungal dose of 10(8) conidia/ml. depending on the temperature and rh regime tested, difference in mortality rates of housefly adult and larvae were detected. during assay on adult housefly, 100 % mortality was achieved at rh, 90 and 100 % while the temperature of 30 °c showed maximum mortality ... | 2013 | 26688637 |
| microbial control of the invasive spiraling whitefly on cassava with entomopathogenic fungi. | the entomopathogenic fungi beauveria bassiana, metarhizium anisopliae, lecanicillium lecanii and isaria fumosorosea were tested for their efficacy in managing the exotic spiraling whitefly aleurodicus dispersus (hemiptera, aleyrodidae) on cassava (manihot esculenta) during 2 seasons (2011-2012 and 2012-2013). the fungi i. fumosorosea and l. lecanii exhibited promising levels of control (> 70% mortality of the a. dispersus population). the percent mortality increased over time in both seasons. ap ... | 2017 | 26691465 |
| toxicity of entomopathogenic fungi, beauveria bassiana and lecanicillium muscarium against a field-collected strain of the german cockroach blattella germanica (l.) (dictyoptera: blattellidae). | the german cockroach, blattella germanica (l.) has been recognized as a serious health problem throughout the world. control failures due to insecticide resistance and chemical contamination of environment have led some researchers focus on the other alternative strategy controls. microbial insecticides such as those containing entomo pathogenic fungi could be of high significance. lecanicillium muscarium and beauveria bassiana grow naturally in soils throughout the world and act as a parasite o ... | 2015 | 26695206 |
| mas5, a homologue of bacterial dnaj, is indispensable for the host infection and environmental adaptation of a filamentous fungal insect pathogen. | mas5, a yeast heat shock protein classified to the hsp40 group, is homologous to bacterial archetype dnaj but functionally unexplored in filamentous fungi. here we identify a mas5 homologue (46.86 kda) in beauveria bassiana and show its indispensability for host infection and environmental adaptation of the fungal insect pathogen. the deletion of mas5 caused severe defects in aerial conidiation, conidial germination and submerged blastospore production (mimic to host haemocoel). the deletion mut ... | 2016 | 26714790 |
| the pal pathway required for ambient ph adaptation regulates growth, conidiation, and osmotolerance of beauveria bassiana in a ph-dependent manner. | the pal/rim pathway essential for fungal adaptation to ambient ph has been unexplored in beauveria bassiana, a classic fungal entomopathogen. here, we show the characterized pal pathway comprising transcription factor pacc and upstream six pal partners (pala/b/c/f/h/i) in b. bassiana. their coding genes were all transcribed most abundantly in standard wild-type culture under the alkaline condition of ph 9. deletion of pacc or each pal gene resulted in a significant delay of culture acidification ... | 2016 | 26754817 |
| molecular tracing of white muscardine in asian corn borer using inter-simple sequence repeat (issr) analysis. | beauveria bassiana is a soil fungus that parasitizes arthropod species, and is used to control the asian corn borer in northeast china. in this study, b. bassiana was investigated in xiaoxian county and baicheng city, and the results were compared with those of gongzhuling city, where the fungus was not applied. using the inter-simple sequence repeat (issr) molecular marker technique, 198 isolates were extracted from asian corn borer and other insect cadavers, and soil and air, and two released ... | 2015 | 26782522 |
| the potential for fungal biopesticides to reduce malaria transmission under diverse environmental conditions. | the effectiveness of conventional malaria vector control is being threatened by the spread of insecticide resistance. one promising alternative to chemicals is the use of naturally-occurring insect-killing fungi. numerous laboratory studies have shown that isolates of fungal pathogens such as beauveria bassiana can infect and kill adult mosquitoes, including those resistant to chemical insecticides.unlike chemical insecticides, fungi may take up to a week or more to kill mosquitoes following exp ... | 2015 | 26792946 |
| establishment of polyethylene-glycol-mediated protoplast transformation for lecanicillium lecanii and development of virulence-enhanced strains against aphis gossypii. | lecanicillium lecanii has been developed as a biopesticide and used in biological control of several agricultural insects. to improve fungal virulence, an optimised polyethylene glycol (peg)-mediated protoplast transformation system was established for l. lecanii. pr1a-like cuticle-degrading protease gene (cdep1) from beauveria bassiana was transferred into l. lecanii, and its resulting activity against aphis gossypii was assessed. | 2016 | 26800336 |
| effects of selected herbicides and fungicides on growth, sporulation and conidial germination of entomopathogenic fungus beauveria bassiana. | the in vitro fungicidal effects of six commonly used fungicides, namely fluazinam, propineb, copper(ii) hydroxide, metiram, chlorothalonil and mancozeb, and herbicides, namely isoxaflutole, fluazifop-p-butyl, flurochloridone, foramsulfuron, pendimethalin and prosulfocarb, on mycelial growth, sporulation and conidial germination of entomopathogenic fungus beauveria bassiana (atcc 74040) were investigated. mycelial growth rates and sporulation at 15 and 25 °c were evaluated on pda plates containin ... | 2016 | 26817532 |
| development of a co2 -releasing coformulation based on starch, saccharomyces cerevisiae and beauveria bassiana attractive towards western corn rootworm larvae. | co2 is known as an attractant for many soil-dwelling pests. to implement an attract-and-kill strategy for soil pest control, co2 -emitting formulations need to be developed. the aim of the present work was to develop a slow-release bead system in order to bridge the gap between application and hatching of western corn rootworm larvae. | 2016 | 26834068 |
| three duf1996 proteins localize in vacuoles and function in fungal responses to multiple stresses and metal ions. | many annotated fungal genomes harbour high proportions of hypothetical proteins with or without domains of unknown function (duf). here, three novel proteins (342-497 amino acids), each containing only a single large duf1996 (231-250 residues) region with highly conserved head (dpixxp) and tail (hxdxxxgw) signatures, were expressed as egfp-tagged fusion proteins and shown to specifically localize in the vacuoles of beauveria bassiana, a filamentous fungal entomopathogen; therefore, these protein ... | 2016 | 26839279 |
| immunity of the greater wax moth galleria mellonella. | investigation of insect immune mechanisms provides important information concerning innate immunity, which in many aspects is conserved in animals. this is one of the reasons why insects serve as model organisms to study virulence mechanisms of human pathogens. from the evolutionary point of view, we also learn a lot about host-pathogen interaction and adaptation of organisms to conditions of life. additionally, insect-derived antibacterial and antifungal peptides and proteins are considered for ... | 2016 | 26847724 |
| integrated pest management of coffee berry borer: strategies from latin america that could be useful for coffee farmers in hawaii. | the coffee berry borer (cbb), hypothenemus hampei ferrari (coleoptera: curculionidae: scolytinae) is the primary arthropod pest of coffee plantations worldwide. since its detection in hawaii (september 2010), coffee growers are facing financial losses due to reduced quality of coffee yields. several control strategies that include cultural practices, biological control agents (parasitoids), chemical and microbial insecticides (entomopathogenic fungi), and a range of post-harvest sanitation pract ... | 2016 | 26848690 |
| potential of entomopathogenic fungi as biological control agents of diamondback moth (lepidoptera: plutellidae) and compatibility with chemical insecticides. | the objectives were to evaluate the efficiency of entomopathogenic fungi against plutella xylostella (l.) and the compatibility of the most virulent isolates with some of the insecticides registered for use on cabbage crops. pathogenicity tests used isolates of beauveria bassiana, metarhizium rileyi, isaria fumosorosea, isaria sinclairii, and lecanicillium muscarium standardized at a concentration of 10(7) conidia/ml. cabbage leaf discs were immersed in these suspensions, and after evaporation o ... | 2016 | 26850733 |
| toxicity of natural insecticides on the larvae of wheat head armyworm, dargida diffusa (lepidoptera: noctuidae). | the wheat head armyworm, dargida (previously faronta) diffusa (walker) (lepidoptera: noctuidae), is widely distributed in north american grasslands and is most common on the great plains, where it is often a serious pest of corn and cereal crops. six commercially available botanical or microbial insecticides used against d. diffusa were tested in the laboratory: entrust(®) wp (spinosad 80%), mycotrol(®) eso (beauveria bassiana gha), aza-direct(®) (azadirachtin), met52(®) ec (metarhizium brunneum ... | 2016 | 26855414 |
| survey of indigenous entomopathogenic fungi and evaluation of their pathogenicity against the carmine spider mite, tetranychus cinnabarinus (boisd.), and the whitefly, bemisia tabaci (genn.) biotype b. | the carmine spider mite, tetranychus cinnabarinus, and the silverleaf whitefly, bemisia tabaci, are serious pests of both field- and greenhouse-grown crops in south-western turkey. control of these pests has been heavily dependent upon chemical pesticides. the objectives of this study were to investigate the occurrence of indigenous entomopathogenic fungi (epf) in field populations of t. cinnabarinus and b. tabaci, and to evaluate their pathogenicity against these pests. for this purpose, a surv ... | 2016 | 26929052 |
| antifungal, antiradical and cytotoxic activities of extractives obtained from tagetes patula l. (asteraceae), a potential acaricide plant species. | tagetes patula l. shows a complex chemical composition, ranging from glycosylated flavonoids and thiophenes in extracts until terpenoids in the essential oil. in the present study, due to this rich flavonoidic constitution, its antioxidant potential was determined, having shown values of antiradical percentage superior to reference compounds, mainly the extracts prepared with flowers. previous studies performed emphasized the acaricide potential of t. patula and thus, the present study aimed to ... | 2016 | 26945559 |
| bombyx mori cecropin a has a high antifungal activity to entomopathogenic fungus beauveria bassiana. | a cdna encoding cecropin a (ceca) was cloned from the larvae of silkworm, bombyx mori, using rt-pcr. it encodes a protein of 63 amino acids, containing a 22 amino acid signal peptide and a 37 amino acid mat peptide of functional domain. the ceca secondary structure contains two typical amphiphilic α-helices. real-time qpcr analysis revealed that ceca was expressed in all the tissues tested, including cuticle, fat body, hemocytes, malpighian tubule, midgut and silk gland in the silkworm larvae wi ... | 2016 | 26945628 |
| transient endophytic colonizations of plants improve the outcome of foliar applications of mycoinsecticides against chewing insects. | the current work reports how spray application of entomopathogenic fungi on alfalfa, tomato and melon plants may cause an additional spodoptera littoralis larvae mortality due to a temporal colonization of the leaves and subsequent ingestion of those leaves by the larvae. most entomopathogenic fungi (ef) (ascomycota: hypocreales) endophytes seem to colonize their host plants in a non-systemic pattern, in which case at least a transient endophytic establishment of the fungus should be expected in ... | 2016 | 26945771 |
| in vivo expression of genes in the entomopathogenic fungus beauveria bassiana during infection of lepidopteran larvae. | the entomopathogenic fungus beauveria bassiana (bals.) vuillemin is commercially available as a bio insecticide. the expression of three genes previously identified to have a role in pathogenicity in in vitro studies was validated in vivo in three lepidopteran insects infected with b. bassiana. expression of all three genes was observed in all the tested insects starting from 48 or 72h to 10d post infection corroborating their role in pathogenicity. we suggest that it is essential to test the ex ... | 2016 | 26945772 |
| functional interaction between apolipophorins and complement regulate the mosquito immune response to systemic infections. | the complement-like protein thioester-containing protein 1 (tep1) is the hallmark effector molecule against plasmodium ookinetes in the malaria vector anopheles gambiae. we have previously shown that the knockdown of the noncatalytic clip domain serine protease clipa2 increased tep1-mediated killing rendering mosquitoes more resistant to plasmodium, bacterial and fungal infections. here, clipa2 coimmunoprecipitation from the hemolymph of beauveria bassiana-infected mosquitoes followed by mass sp ... | 2016 | 26950600 |
| regulative roles of glutathione reductase and four glutaredoxins in glutathione redox, antioxidant activity, and iron homeostasis of beauveria bassiana. | multiple glutaredoxins (grx) and glutathione reductase (glr) are vital for the thiol-disulfide redox system in budding yeast but generally unexplored in filamentous fungi. here we characterized the beauveria bassiana redox system comprising dithiol grx1, monothiol grx2-4, grx-like grx5, and glr orthologue. each grx or glr deletion was compensated by increased transcripts of some other grx genes in normal cultures. particularly, grx3 compensated the absence of grx1, grx2, grx5, or glr under oxida ... | 2016 | 26969041 |
| development of a method for detection and quantification of b. brongniartii and b. bassiana in soil. | a culture independent method based on qpcr was developed for the detection and quantification of two fungal inoculants in soil. the aim was to adapt a genotyping approach based on ssr (simple sequence repeat) marker to a discriminating tracing of two different species of bioinoculants in soil, after their in-field release. two entomopathogenic fungi, beauveria bassiana and b. brongniartii, were traced and quantified in soil samples obtained from field trials. these two fungal species were used a ... | 2016 | 26975931 |
| subcellular localization of five singular wsc domain-containing proteins and their roles in beauveria bassiana responses to stress cues and metal ions. | some model fungi have three or four proteins with each vectoring a single cell wall stress-responsive component (wsc) domain at n-terminus. in this study, five proteins, each vectoring only a single wsc domain in n-terminal, central or even c-terminal region, were found in beauveria bassiana, a filamentous fungal entomopathogen, and named wsc1a-1e due to the domain singularity. four of them lack either transmembrane domain or c-terminal conserved signature sequence (dxxd) compared with the homol ... | 2016 | 26994521 |
| proteins in the cocoon of silkworm inhibit the growth of beauveria bassiana. | silk cocoons are composed of fiber proteins (fibroins) and adhesive glue proteins (sericins), which provide a physical barrier to protect the inside pupa. moreover, other proteins were identified in the cocoon silk, many of which are immune related proteins. in this study, we extracted proteins from the silkworm cocoon by tris-hcl buffer (ph7.5), and found that they had a strong inhibitory activity against fungal proteases and they had higher abundance in the outer cocoon layers than in the inne ... | 2016 | 27032085 |
| insight into the feeding behavior of predatory mites on beauveria bassiana, an arthropod pathogen. | interactions between fungal entomopathogens and pest predators are particularly relevant in control of agricultural insect pests. in a laboratory study, we confirmed that the predatory mite, neoseiulus barkeri, exhibited feeding behavior on the entomopathogenic fungus beauveria bassiana conidia through dna extracts. using transmission electron microscopy, we determined that the majority of conidia found in the mite gut tended to dissolve within 24 h post ingestion, suggesting that the conidia ha ... | 2016 | 27041703 |
| genome-wide host-pathogen interaction unveiled by transcriptomic response of diamondback moth to fungal infection. | genome-wide insight into insect pest response to the infection of beauveria bassiana (fungal insect pathogen) is critical for genetic improvement of fungal insecticides but has been poorly explored. we constructed three pairs of transcriptomes of plutella xylostella larvae at 24, 36 and 48 hours post treatment of infection (hpti) and of control (hptc) for insight into the host-pathogen interaction at genomic level. there were 2143, 3200 and 2967 host genes differentially expressed at 24, 36 and ... | 2016 | 27043942 |
| fungal biological control agents for integrated management of culicoides spp. (diptera: ceratopogonidae) of livestock. | entomopathogenic fungi metarhizium anisopliae and beauveria bassiana had wide host range against insects and hence these are being exploited as fungal bio-pesticide on a large scale. both fungi are proved pesticides against many crop pests and farmers are well acquainted with their use on the field. thus, research was aimed to explore the potency of these fungal spores against larval and adult culicoides midges, a pest of livestock. | 2015 | 27047065 |
| proteomic and phosphoproteomic insights into a signaling hub role for cdc14 in asexual development and multiple stress responses in beauveria bassiana. | cdc14 is a dual-specificity phosphatase that regulates nuclear behavior by dephosphorylating phosphotyrosine and phosphoserine/phosphothreonine in fungi. previously, cdc14 was shown to act as a positive regulator of cytokinesis, asexual development and multiple stress responses in beauveria bassiana, a fungal insect pathogen. this study seeks to gain deep insight into a pivotal role of cdc14 in the signaling network of b. bassiana by analyzing the cdc14-specific proteome and phosphoproteome gene ... | 2016 | 27055109 |
| laboratory evaluation of beauveria bassiana and metarhizium anisopliae in the control of haemaphysalis qinghaiensis in china. | haemaphysalis qinghaiensis, a prevalent tick species in china, is an ectoparasite that preferentially infests small ruminants and can transmit theileria sp. and babesia sp. in this study, we evaluated the pathogenicity of individual and mixed infections of the fungi beauveria bassiana and metarhizium anisopliae to h. qinghaiensis nymphs. the estimated lc50 for ticks immersed in solutions of b. bassiana, m. anisopliae and a mixture thereof were: 5.88056 × 10(4), 2.65 × 10(4), and 2.85 × 10(4) con ... | 2016 | 27071674 |
| the efficacy of beauveria bassiana, jasmonic acid and chlorantraniliprole on larval populations of helicoverpa armigera in chickpea crop ecosystems. | a robust integrated pest management (ipm) programme is needed to reduce the use of insecticides in controlling helicoverpa armigera. therefore, a 2 year field study was conducted to evaluate the use of alternative control measures (biochemical use) for h. armigera relative to exclusively using chemical insecticides. the entomopathogenic fungus beauveria bassiana, jasmonic acid and the insecticide chlorantraniliprole were each applied twice during the chickpea growing season. | 2017 | 27101405 |
| beauveria bassiana and metarhizium anisopliae endophytically colonize cassava roots following soil drench inoculation. | we investigated the fungal entomopathogens beauveria bassiana and metarhizium anisopliae to determine if endophytic colonization could be achieved in cassava. an inoculation method based on drenching the soil around cassava stem cuttings using conidial suspensions resulted in endophytic colonization of cassava roots by both entomopathogens, though neither was found in the leaves or stems of the treated cassava plants. both fungal entomopathogens were detected more often in the proximal end of th ... | 2016 | 27103778 |
| reactive oxygen species production, induced by atmospheric modification, alter conidial quality of beauveria bassiana. | the aim of this study was to determine the relationship between reactive oxygen species (ros) production and conidial infectivity in beauveria bassiana. | 2016 | 27107399 |
| the na(+) /h(+) antiporter nhx1 controls vacuolar fusion indispensible for life cycles in vitro and in vivo in a fungal insect pathogen. | the sole na(+) /h(+) antiporter nhx1 has been generally unexplored in filamentous fungi. we characterized nhx1 in the entomopathogenic fungus beauveria bassiana. an egfp-tagged nhx1 fusion accumulated in small punctuate structures, presumably endosomal and trans-golgi network compartments, between septum and tubular vacuole of each wild-type cell stained with a vacuole-specific dye. deletion of nhx1 resulted in significant acidification and severe fusion defect in vacuoles, which were fragmented ... | 2016 | 27112237 |
| metabolic responses of beauveria bassiana to hydrogen peroxide-induced oxidative stress using an lc-ms-based metabolomics approach. | the entomopathogenic fungus, beauveria bassiana, is commonly used as a biological agent for pest control. environmental and biological factors expose the fungus to oxidative stress; as a result, b. bassiana has adopted a number of anti-oxidant mechanisms. in this study, we investigated metabolites of b. bassiana that are formed in response to oxidative stress from hydrogen peroxide (h2o2) by using a liquid chromatography mass spectrometry (lc-ms) approach. partial least-squares discriminant anal ... | 2016 | 27116916 |
| dissection of the contributions of cyclophilin genes to development and virulence in a fungal insect pathogen. | cyclophilins are ubiquitous proteins found in all domains of life, catalyzing peptidyl-prolyl cis-trans isomerization (ppiase activity) and functioning in diverse cellular processes. the filamentous insect pathogenic fungus, beauveria bassiana, contains 11 cyclophilin genes whose roles were probed via individual gene knockouts, construction of over-expression strains, and a simultaneous gene knockdown strategy using tandem sirna. mutants were examined for effects on conidiation, hyphal growth, c ... | 2016 | 27130487 |
| advances in genomics of entomopathogenic fungi. | fungi are the commonest pathogens of insects and crucial regulators of insect populations. the rapid advance of genome technologies has revolutionized our understanding of entomopathogenic fungi with multiple metarhizium spp. sequenced, as well as beauveria bassiana, cordyceps militaris, and ophiocordyceps sinensis among others. phylogenomic analysis suggests that the ancestors of many of these fungi were plant endophytes or pathogens, with entomopathogenicity being an acquired characteristic. t ... | 2016 | 27131323 |
| insect pathogenic fungi as endophytes. | in this chapter, we explore some of the evolutionary, ecological, molecular genetics, and applied aspects of a subset of insect pathogenic fungi that also have a lifestyle as endophytes and we term endophytic insect pathogenic fungi (eipf). we focus particularly on metarhizium spp. and beauveria bassiana as eipf. the discussion of the evolution of eipf challenges a view that these fungi were first and foremost insect pathogens that eventually evolved to colonize plants. phylogenetic evidence sho ... | 2016 | 27131324 |