Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| human herpesvirus 6 in non-aids related hodgkin's and non-hodgkin's lymphomas. | 1992 | 1318476 | |
| [3-day fever: agreement between clinical diagnosis and seroconversion of type 6 human herpesvirus (hhv-6)]. | the aim of this study was to investigate the correlation between clinical diagnosis of exanthema subitum and seroconversion of herpes virus type 6 (hhv-6). 90 children with fever of unknown origin were investigated by 11 pediatricians in the canton of zürich, switzerland, between october 1989 and june 1990. antibodies against hhv-6 were determined at the first consultation and 2-3 weeks later. history and clinical findings were investigated and the clinical probability of the diagnosis exanthema ... | 1992 | 1318578 |
| human herpesvirus-6 infection and renal transplantation. | 1992 | 1318588 | |
| effect of repeated intradermal injections of bovine herpesvirus type 1 antigen on seronegative cattle. | forty-three cattle seronegative to bovine herpesvirus-1 (bhv-1) were given from one to five intradermal injections of bhv-1 inactivated antigen at four-week intervals. this delayed hypersensitivity test was assessed by the increase in skin thickness. the activity of the antigen was assessed in five animals which had a previous natural bhv-1 infection with clinical signs and seroconversion. anti-bhv-1 antibodies were detected by seroneutralisation and an enzyme-linked immunoassay. only one animal ... | 1992 | 1318596 |
| the dna sequence of equine herpesvirus-1. | the complete dna sequence was determined of a pathogenic british isolate of equine herpesvirus-1, a respiratory virus which can cause abortion and neurological disease. the genome is 150,223 bp in size, has a base composition of 56.7% g + c, and contains 80 open reading frames likely to encode protein. since four open reading frames are duplicated in the major inverted repeat, two are probably expressed as a spliced mrna, and one may contain an internal transcriptional promoter, the genome is co ... | 1992 | 1318606 |
| the pathogenicity of ab4p, the sequenced strain of equine herpesvirus-1, in specific pathogen-free foals. | the sequencing of the genome of equine herpesvirus-1 (ehv-1) is reported in elizabeth a. r. telford, moira s. watson, kathryn mcbride, and andrew j. davison, 1992, virology, 189, 304-316. the sequence was derived using a plaque-purified clone of ehv-1 strain ab4 (designated ab4p). to ensure that ab4p shares the pathogenic characteristics of parental ab4 (hereafter ab4), both were inoculated intranasally into foals, specifically free from ehv-1 and ehv-4. clinical signs, including rectal temperat ... | 1992 | 1318607 |
| cloning and restriction endonuclease mapping of the genome of an equine herpesvirus 4 (equine rhinopneumonitis virus), strain 405/76. | purified virion dna of an australian isolate of equine herpesvirus 4(ehv 4.405/76) was digested with restriction enzymes and the dna fragments were cloned into puc19. the resulting recombinant plasmid library, representing 92% of the virus genome, was used in hybridization analyses to construct restriction maps for bamhi, ecori, and sali for the ehv4 genome. the results show that the genome of ehv 4.405/76 was approximately 145 kb and comprised a unique long (ul) region of 112 kb and a unique sh ... | 1992 | 1318713 |
| foscarnet therapy for aids-related opportunistic herpesvirus infections. | 1992 | 1318736 | |
| epizootiological aspects of type 1 and type 4 equine herpesvirus infections among horse populations. | the dissemination of equine herpesvirus types 1 (ehv-1) and 4 (ehv-4) among various horse populations in japan was investigated through the isolation and typing of virus strains from horses with respiratory diseases. type specific monoclonal antibody pools were used for the typing of isolates. the 42 strains of ehv-1 and 64 strains of ehv-4 were isolated from 4593 nasal swabs and/or blood plasma samples collected from 3326 horses during a period from 1979 to 1990. all the strains of ehv-1 were i ... | 1992 | 1318750 |
| replication of human herpesvirus 6 in epithelial cells in vitro. | mink lung epithelial (nbl-7) cells were shown to be permissive for human herpesvirus 6 (hhv-6) by four independent methods of analysis: detection of infectious virus, viral antigens, viral dna sequences, and herpesvirus particles. infection was serially passaged, with minimal cytopathology, for several months demonstrating for the first time that a cell of epithelial origin can support hhv-6 replication. | 1992 | 1318910 |
| evaluation of variables in immunofluorescence procedures for the detection of antibodies against human herpesvirus 6 (hhv-6). | ten human sera were used to study different parameters, namely, methods of smear preparation and fixation, and age of infected hsb-2 cells in order to optimize indirect immunofluorescence assay (ifa) and anticomplement immunofluorescence (acif) procedures to measure antibody levels against hhv-6. results showed a greater sensitivity of rapid smear drying and methanol fixation over conventional acetone smear preparation. cells harvested 6 days after infection and fixed with methanol exhibited a s ... | 1992 | 1319300 |
| a monkey model for epstein barr virus-associated lymphomagenesis in human acquired immunodeficiency syndrome. | high-grade malignant nonhodgkin's lymphomas--five lymphoblastic, three pleomorphic, and two immunoblastic--developed in 10/25 cynomolgus monkeys (macaca fascicularis) followed for up to 746 d after infection with simian immunodeficiency virus, strain sivsm. these lymphomas were shown to be associated with an epstein-barr (eb)-like cynomolgus b-lymphotropic herpesvirus (cblv) by electron microscopy, by southern blot hybridization with probes against human ebv, and by the expression of antigens co ... | 1992 | 1319458 |
| [what is the relation of human herpesvirus 6 to chronic fatigue syndrome?]. | 1992 | 1319541 | |
| human cytomegalovirus ul97 open reading frame encodes a protein that phosphorylates the antiviral nucleoside analogue ganciclovir. | human cytomegalovirus (hcmv, a betaherpes virus) is the cause of serious disease in immunologically compromised individuals, including those with acquired immunodeficiency syndrome. one of the compounds used in the chemotherapy of hcmv infections is the nucleoside analogue 9-(1,3-dihydroxy-2-propoxymethyl)-guanine (ganciclovir). the mechanism of action of this drug is dependent on the formation of the nucleoside triphosphate, which is a strong inhibitor of the viral dna polymerase. thymidine kin ... | 1992 | 1319559 |
| psychosocial modulation of antibody to epstein-barr viral capsid antigen and human herpesvirus type-6 in hiv-1-infected and at-risk gay men. | we investigated the effects of two behavioral interventions--aerobic exercise and cognitive behavioral stress management (cbsm)--on epstein-barr virus viral capsid antigen (ebv-vca) and human herpesvirus type-6 (hhv-6) antibody modulation in 65 asymptomatic gay men measured at several time points in the 5 weeks preceding and following notification of their human immunodeficiency virus-type 1 (hiv-1) serostatus. after accounting for potential immunomodulatory confounds, we found that hiv-1 seropo ... | 1992 | 1320279 |
| newest human herpesvirus (hhv-6) in the guillain-barré syndrome and other neurological diseases. | to investigate the presence of human herpesvirus-6 (hhv-6) in patients affected by guillain-barré syndrome (gbs) and by other neurological diseases (ond), we examined by indirect immunofluorescence analysis (ifa) the sera and cerebrospinal fluid (csf) from 28 gbs and 63 ond. moreover, we tested 150 blood donors (bd) to appreciate the diffusion of hhv-6 infection in the italian adult healthy population. we found a significantly higher titre of antibody to hhv-6 in the gbs patients compared with o ... | 1992 | 1320318 |
| intracellular synthesis of varicella-zoster virus. | varicella-zoster virus, a reclusive alpha-herpesvirus, can be isolated and propagated in a limited number of human cell lines. under in vitro conditions, the virus is strongly cell-associated and, therefore, difficult to recover in a truly cell-free state. the general replication cycle includes the three phases called immediate early, early, and late. an example of an early viral protein is a putative protein kinase called open reading frame (orf) 47. an example of a late protein is the preponde ... | 1992 | 1320653 |
| anti-hhv-6 antibodies in normal population and in cancer patients in india. | the prevalence and titre of igg antibodies to human herpesvirus type 6 (hhv-6) were assayed in the serum samples from normal subjects and patients with hodgkin's lymphoma (hl), non-hodgkin's lymphoma (nhl), acute lymphoblastic leukaemia (all) and oral cancer (oc) using immunofluorescence and immunoperoxidase techniques. this forms the first study on the sero-prevalence and titre of antibodies to hhv-6 in india. there was no considerable difference in the prevalence (76%) and titre (10-160) of th ... | 1992 | 1320669 |
| the molecular epidemiology of equine herpesvirus 1 (equine abortion virus) in australasia 1975 to 1989. | the restriction endonuclease dna fingerprints of 57 isolates of equine herpesvirus 1 (ehv1; equine abortion virus) from abortion, perinatal foal mortalities and encephalitis from 15 epidemics that occurred in australasia between 1975 and 1989 were examined using the enzymes bam hi, ecori and bgl ii. there was a remarkable degree of uniformity in the restriction patterns; mobility differences were observed in only 14 of 52 (27%) of the fragments. twelve of these 14 fragments were located within t ... | 1992 | 1320856 |
| influence of turkey herpesvirus vaccination on the b-haplotype effect on marek's disease resistance in 15.b-congenic chickens. | eight recently developed 15.b congenic lines of chickens were tested for marek's disease (md) resistance by intra-abdominal injection of cell-associated preparations of md virus of a virulent strain (jm), a very virulent strain (md5), or md5 after vaccination with turkey herpesvirus (hvt) strain fc126. chickens of the 15.n congenic line (b15b21 or b21b21) were very resistant to jm-induced md, in contrast to chickens homozygous for the b-haplotypes 2, 5, 12, 13, 15, or 19. after md5 infection, mo ... | 1992 | 1320867 |
| the right end of the unique region of the genome of human herpesvirus 6 u1102 contains a candidate immediate early gene enhancer and a homologue of the human cytomegalovirus us22 gene family. | the nucleotide sequence of a 12 kbp hindiii fragment (hindiii c) from the right end of the unique component of the genome of human herpesvirus 6 (hhv-6) (strain u1102) was determined. the sequence has a mean g + c content of 42% and contains approximately 28 copies of a tandemly repeated 104 to 107 bp element, which, with a single exception, contain a cleavage site for kpni (the kpni repeats). each of these elements contains potential binding sites for transcription factors nf-kappa b and ap2. t ... | 1992 | 1321205 |
| identification of homologues to the human cytomegalovirus us22 gene family in human herpesvirus 6. | the sequence of 10079 bp corresponding to the overlapping sali h and smai g restriction fragments of the genome of human herpesvirus 6 (hhv-6) strain u1102 was determined. the sequence contains six complete open reading frames (orfs) and two incomplete orfs located at the 5' and 3' ends of the sali h and smai g fragments respectively. seven of these orfs have recognizable homologues only in the beta-herpesvirus human cytomegalovirus (hcmv), no obvious counterparts being detectable in the genomes ... | 1992 | 1321206 |
| experimental infection of cynomolgus and african green monkeys with human herpesvirus 6. | cynomolgus and african green monkeys were inoculated with human herpesvirus 6 (hhv-6). an antibody response was first observed 10 days and 5 days after inoculation of cynomolgus monkeys and african green monkeys, respectively, and was detectable for the duration of the experiment (33 days). hhv-6 dna was first detected by the polymerase chain reaction in mononuclear cells of one cynomolgus monkey and one african green monkey 10 days after virus inoculation, and in a total of three of four cynomo ... | 1992 | 1321207 |
| the use of feline herpesvirus and baculovirus as vaccine vectors for the gag and env genes of feline leukaemia virus. | the env and gag genes from feline leukaemia virus were expressed in a thymidine kinase-negative feline herpes-virus and a baculovirus. cats were vaccinated with various combinations of these recombinant viruses and 100% protection against feline leukaemia virus challenge was achieved using an immunization schedule which utilized both env and gag products delivered at both a mucosal and systemic site. | 1992 | 1321214 |
| identification of the glycoprotein h gene of murine cytomegalovirus. | partial sequencing of the hindiii c fragment of murine cytomegalovirus (mcmv) revealed an open reading frame of 2172 nucleotides in length encoding a 724 amino acid protein with a predicted mr of 80.4k. analysis of the predicted amino acid sequence revealed homology with glycoprotein h (gh) from a number of other herpesviruses. mcmv gh showed strongest amino acid identity with human (h) cmv and human herpesvirus 6 gh, and less identity with the gh protein sequences of epstein-barr virus, varicel ... | 1992 | 1321219 |
| primary structure of the herpesvirus saimiri genome. | this report describes the complete nucleotide sequence of the genome of herpesvirus saimiri, the prototype of gammaherpesvirus subgroup 2 (rhadinoviruses). the unique low-g + c-content dna region has 112,930 bp with an average base composition of 34.5% g + c and is flanked by about 35 noncoding high-g + c-content dna repeats of 1,444 bp (70.8% g + c) in tandem orientation. we identified 76 major open reading frames and a set of seven u-rna genes for a total of 83 potential genes. the genes are c ... | 1992 | 1321287 |
| natural killer cells in normal horses and specific-pathogen-free foals infected with equine herpesvirus. | peripheral blood mononuclear cells (pbmc) from an adult horse and from foals demonstrated natural killer (nk)-type cytotoxicity against a range of xenogeneic and allogeneic cell targets. the human tumour cell line, chang liver was consistently the most susceptible. chang liver, rabbit kidney (rk-13), equine sarcoid (es) and embryonic equine kidney (eek) cells were more susceptible when presented to horse pbmc than monolayer cultures. embryonic equine lung (eel) and murine yac-1 cells conversely, ... | 1992 | 1321530 |
| detection of human herpesvirus 6 in tissues involved by sinus histiocytosis with massive lymphadenopathy (rosai-dorfman disease). | after preliminary serologic data demonstrated elevated antibody titers to human herpesvirus (hhv) 6 in patients with sinus histiocytosis with massive lymphadenopathy (shml) or rosai-dorfman disease, tissues were examined from 9 patients with classical shml to search for evidence of hhv-6 infection. involved tissues from 7 of the 9 patients had detectable hhv-6 by in situ hybridization: tissue from 1 had detectable epstein-barr virus genome but no hhv-6 and tissue from another had no detectable h ... | 1992 | 1321861 |
| glycoprotein 60 of equine herpesvirus type 1 is a homologue of herpes simplex virus glycoprotein d and plays a major role in penetration of cells. | monoclonal antibodies (mabs) specific for equine herpesvirus type 1 (ehv-1) glycoprotein 60 (gp60) and gp 17/18 (f3132 and 5h6 respectively) were found to react with the same protein, which was identified as a homologue of herpes simplex virus type 1 gd. mab f3132 strongly neutralized virus infectivity and inhibited the penetration of the virus into the cell. the effects on penetration were shared with three other mabs against this protein (p68, f3116 and f3129), but no effect on virus penetrati ... | 1992 | 1321875 |
| human herpesvirus-6: clinical implications of a recently discovered, ubiquitous agent. | 1992 | 1322455 | |
| sequence and transcript analysis of the bovine herpesvirus 1 thymidine kinase locus. | a detailed sequence and transcript analysis of the thymidine kinase (tk) gene of bovine herpesvirus 1 (bhv1, cooper strain) was carried out. a tk open reading frame (orf) of 1077 bp was identified and compared with tk orfs previously published for other strains of bhv1. the cooper sequence was in good agreement with that of strain q3932 but differed significantly from strains 6660 and la. reanalysis of the la tk sequence, however, failed to confirm this difference. except for five single base su ... | 1992 | 1322582 |
| an in vivo study of a glycoprotein giii-negative bovine herpesvirus 1 (bhv-1) mutant expressing beta-galactosidase: evaluation of the role of giii in virus infectivity and its use as a vector for mucosal immunization. | we constructed a recombinant bhv-1 in which the glycoprotein giii gene was replaced by the escherichia coli lacz gene. the resultant virus mimics the simple giii deletion mutant in its growth characteristics in cell culture; however, it expresses beta-galactosidase in virus-infected cells. further characterization of its virulence and the immune responses elicited by it was conducted in cattle. the mutant virus retained the ability to establish an infection when administered intranasally. infect ... | 1992 | 1322592 |
| absence of antibodies to human herpesvirus-6 in patients with slowly-progressive human immunodeficiency virus type 1 infection. | to evaluate a possible role for human herpesvirus-type 6 (hhv-6) coinfection as a co-factor in the progression of hiv-1 disease, we investigated the prevalence of seropositivity for hhv-6 in a cohort of hiv-1 infected patients. these patients were retrospectively divided into two groups according to the decline of cd4+ t cells during the follow up: 11 were classified as rapid decliners (less than 400 cd4+/cmm within 1 year), and 38 as slow decliners (greater than 400 cd4+/cmm after at least 4 ye ... | 1992 | 1322823 |
| the detection of alcelaphine herpesvirus-1 dna by in situ hybridization of tissues from rabbits affected with malignant catarrhal fever. | tissue sections and cultured lymphocytes from rabbits clinically affected following experimental infection with alcelaphine herpesvirus-1 (ahv-1) were assessed for the presence of viral dna by in situ hybridization with the cloned major hindii repeat sequence of this virus. small numbers of virus-infected cells were consistently detected only in submandibular lymph nodes, while other tissues showed no evidence of viral dna. virus titration in culture suggested that there were higher titres of vi ... | 1992 | 1322946 |
| mechanisms of bovine herpesvirus type 1 neutralization by monoclonal antibodies to glycoproteins gi, giii and giv. | we examined a panel of monoclonal antibodies (mabs) against bovine herpesvirus type 1 (bhv-1) glycoproteins gi, giii and giv for inhibition of virus attachment and interference with subsequent steps of infection. attachment of radiolabelled virions was partially prevented by 600 to 700 micrograms/ml of igm antibodies against gi and giii and one igg2a antibody against giv, but not by the majority of mabs against any of the three viral glycoproteins. productive infection following attachment was p ... | 1992 | 1322960 |
| evidence for putative immediate early antigens in human herpesvirus 6-infected cells. | human herpesvirus 6 (hhv-6) induced nuclear antigens in cells as early as 3 h after infection. these nuclear antigens were induced by all three strains of hhv-6 tested, and their de novo synthesis required the function(s) of the intact viral genome. their appearance was not affected by 2,2'-anhydro(1-beta-d-arabinofuranosyl)cytosine, but was completely inhibited by cycloheximide. however, the nuclear antigens did appear if cycloheximide was replaced with actinomycin d. thus, the nuclear antigens ... | 1992 | 1322964 |
| a novel herpes simplex virus gene (ul49a) encodes a putative membrane protein with counterparts in other herpesviruses. | comparative analysis of dna sequences located between the coding regions of genes ul49 and ul50 of herpes simplex virus types 1 and 2 (hsv-1 and -2) has revealed a small open reading frame (orf) of 91 and 87 codons respectively with the characteristics of a genuine protein-coding region. the predicted protein products are clearly related and exhibit features of membrane-inserted proteins, with potential n-proximal signal peptides and c-proximal membrane anchor regions. counterparts are present i ... | 1992 | 1322965 |
| clinical, epidemiologic, and virologic studies in four clusters of the chronic fatigue syndrome. | the purpose of this study is to provide a case definition of chronic fatigue syndrome in an outbreak occurring in the nevada-california region to evaluate candidate etiologic agents and observe the natural history of the illness. | 1992 | 1323246 |
| equid herpesvirus abortion--another piece in the pathogenesis puzzle. | 1992 | 1323456 | |
| abortion of virologically negative foetuses following experimental challenge of pregnant pony mares with equid herpesvirus 1. | from 1988 to 1991, 51 pregnant pony mares were challenged intranasally or by aerosol with an isolate of ehv-1 (ab4) originally recovered from a quadriplegic mare. this resulted in 32 abortions, occurring from 9 to 29 days after infection. in 14 of the early abortions (days 9-14), ehv-1 was not demonstrated in the foetal tissues by virus isolation or immunostaining despite no other non-viral cause for the abortion being evident. application of the polymerase chain reaction to foetal tissues from ... | 1992 | 1323457 |
| characterization of human herpesvirus 6 strains isolated from patients with exanthem subitum with or without cutaneous rash. | 1992 | 1323627 | |
| human herpesvirus 7 (hhv-7) strain ji: independent confirmation of hhv-7. | 1992 | 1323628 | |
| isolation and identification of psittacid herpesvirus 1 from imported psittacines in south africa. | acute deaths occurred in 47 out of a total of 131 imported psittacine birds whilst in quarantine. few and non-specific clinical signs were seen during the course of the disease outbreak, but gross pathology revealed severe hepatomegaly and splenomegaly. a herpes virus was isolated from liver and spleen material taken from 2 birds, an amazon (amazona aestiva aestiva) and a yellow-collared macaw (ara auricollis). identification procedures included virus neutralisation tests carried out in chicken ... | 1992 | 1323676 |
| identification and transcriptional analyses of the ul3 and ul4 genes of equine herpesvirus 1, homologs of the icp27 and glycoprotein k genes of herpes simplex virus. | the dna sequence of 3,240 nucleotides of the xbai g fragment located in the unique long (ul) region of the equine herpesvirus 1 genome revealed two major open reading frames (orfs) designated ul3 and ul4. the ul3 orf of 470 amino acids (aa) maps at nucleotides (nt) 4450 to 3038 from the long terminus, and its predicted 51.4-kda protein product exhibits significant homology to the icp27 alpha regulatory protein of herpes simplex virus type 1 (hsv-1; 32% identity) and to the orf4 protein of varice ... | 1992 | 1323700 |
| the epstein-barr virus r transactivator (rta) contains a complex, potent activation domain with properties different from those of vp16. | rta, encoded by epstein-barr virus (ebv), is a potent activator of transcription via enhancer sequences located upstream of several viral genes. to identify the domains of rta that facilitate transcription by interacting with cellular transcription factors, different segments of rta were linked to the dna binding domain of yeast transactivator gal4 (residues 1 to 147). these gal4-rta fusion proteins were tested in transfected cells for their ability to activate the adeno e1b promoter with an ups ... | 1992 | 1323708 |
| the cdna of ul15, a highly conserved herpes simplex virus 1 gene, effectively replaces the two exons of the wild-type virus. | the ul15 gene of herpes simplex virus 1 (hsv-1) is encoded by two or more exons in all herpesvirus genomes sequenced to date. the ul15 coding region is highly conserved, and the intron invariably encodes other genes transcribed antisense to the ul15 coding region. previously we reported that we deleted the intron domain encoding ul16 but were unable to delete ul15 (j. d. baines and b. roizman, j. virol. 65:938-944, 1991). here we report that we replaced exon i of ul15 with an unspliced cdna copy ... | 1992 | 1323715 |
| recombinant pseudorabies virus carrying a plasmodium gene: herpesvirus as a new live viral vector for inducing t- and b-cell immunity. | in balb/c mice, the sterile protective immunity induced by immunization with radiation-attenuated plasmodium yoelii sporozoites is eliminated by in vivo depletion of cd8+ t lymphocytes, suggesting that cytotoxic t lymphocytes (ctl) against malaria antigens expressed on infected hepatocytes are required for mediating this protective immunity. to produce a vaccine that would induce ctl against the p. yoelii circumsporozoite protein (cs), we constructed an attenuated pseudorabies virus (prv) contai ... | 1992 | 1323900 |
| treatment of herpesvirus infections in hiv-infected individuals. | to discuss strategies available for the treatment of herpesvirus infections in individuals infected with hiv. | 1992 | 1324033 |
| [seroepidemiologic study of human herpesvirus-6 in intravenous drug addicts, with and without infection caused by human immunodeficiency virus type 1]. | the human herpesvirus-6 (hhv-6) may be a cofactor of infection by the human immunodeficiency virus type 1 (hiv-1). however, there are discrepancies with respect to the possible epidemiological relation between both viruses. the aim of the present study was to study the prevalence of infection by the hhv-6 in intravenous drug addicts (ivda) with and without hiv-1 infection. | 1992 | 1324385 |
| vaccination of mares against equine herpesvirus-1. | 1992 | 1324543 | |
| human herpesvirus 6 (hhv-6) infection in the central nervous system. | human herpesvirus 6 (hhv 6) was isolated from patients with exanthema subitum (es) with a high frequency, and it is now believed that this virus causes es as a primary infection in childhood. hhv 6 infection is highly prevalent in early childhood and this virus may infect infants through the saliva mainly from mother to child. hhv 6 has a tropism to cd4+ cells and destroys cells in vitro. although children recover from es without any sequelae, neurological symptoms associated with exanthema subi ... | 1992 | 1324560 |
| inactivators of herpes simplex virus ribonucleotide reductase: hematological profiles and in vivo potentiation of the antiviral activity of acyclovir. | a1110u (bw 1110u81) is an inactivator of herpesvirus ribonucleotide reductases and a potentiator of the antiviral activity of acyclovir (acv) (t. spector, j. a. harrington, r. w. morrison, jr., c. u. lambe, d. j. nelson, d. r. averett, k. biron, and p. a. furman, proc. natl. acad. sci. usa 86:1051-1055, 1989) that was subsequently found to cause hematological toxicity at high oral doses in rats. eleven structurally related inactivators of herpes simplex virus (hsv) ribonucleotide reductase were ... | 1992 | 1324641 |
| igm neutralizing antibody responses to human herpesvirus-6 in patients with exanthem subitum or organ transplantation. | the assay for detecting igm neutralizing (nt) antibody activity to human herpesvirus-6 (hhv-6) was developed by using pretreatment of blood sample with staphylococcal protein a. the activity was mostly present in igm fractions of serum but not in iga fractions separated by ultracentrifugation. the assay was used for seroepidemiological studies for hhv-6 infection. in primary hhv-6 infection, igm nt antibodies appeared 5 to 7 days after onset of exanthem subitum, reached maximum titers at 2 to 3 ... | 1992 | 1325026 |
| isolation of bovine herpesvirus 1 from preputial swabs and semen of bulls with balanoposthitis. | 1992 | 1325195 | |
| genetic relationships between bovine herpesvirus 4 and the gammaherpesviruses epstein-barr virus and herpesvirus saimiri. | the overall arrangement of genes in the unique central part of the bovine herpesvirus type 4 (bhv-4) genome has been deduced by analysis of short dna sequences. twenty-three genes conserved in at least one of the completely sequenced herpesviruses have been identified and localized. all of these genes encoded amino acid sequences with higher similarity to proteins of the gammaherpesviruses epstein-barr virus (ebv) and herpesvirus saimiri (hvs) than to the homologous products of the alphaherpesvi ... | 1992 | 1325698 |
| bovine herpesvirus 1 as a live virus vector for expression of foreign genes. | dna sequence analysis and 5'-end mapping of mrna were used to identify and clone dna fragments which contain the presumptive promoter (pgl) and poly(a) site (an) of the bovine herpesvirus 1 (bhv1) gl glycoprotein. to confirm the presence of these regulatory regions in the above fragments, they were cloned together with a chloramphenicol acetyltransferase (cat) reporter gene into puc19. the recombinant plasmid formed, pec3, was capable of inducing cat activity when transfected into bovine cells d ... | 1992 | 1325699 |
| sequencing and 5'- and 3'-end transcript mapping of the gene encoding the small subunit of ribonucleotide reductase from bovine herpesvirus type-1. | the complete nucleotide sequence of the gene encoding the small subunit of ribonucleotide reductase (rnr) from bovine herpesvirus type-1 (bhv-1) was determined. the genomic dna fragment sequenced also represented regions corresponding to the carboxy termini of rnr large subunit and of a virion protein causing host shut-off. the small subunit polypeptide was constituted of 314 amino acid residues totalling 35.25 kda. the major transcription initiation and termination sites of the small subunit mr ... | 1992 | 1325701 |
| carbohydrates in herpesvirus infections. | carbohydrate structures constitute important factors in the pathogenesis of herpesvirus infections for three reasons: (i) human herpesviruses specify several biologically active glycoproteins, where co-translational addition of carbohydrate units is essential for generation of functioning viral glycoproteins. (ii) cellular carbohydrate structures are parts of the cellular receptor complex at least for some herpes-viruses. (iii) peripheral carbohydrate structures are engaged in modulation of the ... | 1992 | 1325817 |
| human herpesvirus 6. | 1992 | 1326076 | |
| herpesvirus 6 infection in young children. | 1992 | 1326080 | |
| in situ detection of human herpesvirus 6 in retinitis associated with acquired immunodeficiency syndrome. | 1992 | 1326231 | |
| evidence of human herpesvirus 6 in sjögren syndrome and sarcoidosis. | 1992 | 1326434 | |
| localization of cis-acting sequences in the latency-related promoter of bovine herpesvirus 1 which are regulated by neuronal cell type factors and immediate-early genes. | bovine herpesvirus 1 (bhv-1) establishes a latent infection in sensory ganglionic neurons of cattle. during a latent infection, a single latency-related (lr) transcript is expressed. this observation suggested that dna sequences in the lr promoter are positively regulated by neural cell type factors. the regulation of the lr gene was examined in neural cells as well as nonneural cells in transient assays. a 258-bp xbai-sphi fragment from the lr promoter cis activated the herpes simplex virus typ ... | 1992 | 1326660 |
| the equine herpesvirus type 1 (ehv-1) homolog of herpes simplex virus type 1 us9 and the nature of a major deletion within the unique short segment of the ehv-1 kya strain genome. | the dna sequence of the short (s) genomic component of the equine herpesvirus type 1 (ehv-1)kya strain has been determined recently in our laboratory. analysis of a 1353-bp bamhi/pvuii clone mapping at the unique short/terminal inverted repeat (us/tr) junction revealed 507 bp of us and 846 bp of tr sequences as well as an open reading frame (orf) that is contained entirely within the us. this orf encodes a potential polypeptide of 219 amino acids that shows significant homology to the us9 protei ... | 1992 | 1326805 |
| structural, functional, and immunological characterization of bovine herpesvirus-1 glycoprotein gl expressed by recombinant baculovirus. | the major glycoprotein complex gl of bovine herpesvirus-1 was expressed at high levels (36 micrograms per 1 x 10(6) cells) in insect cells using a recombinant baculovirus. the recombinant gl had an apparent molecular weight of 116 kda and was partially cleaved to yield 63-kda (glb) and 52-kda (glc) subunits. this processing step was significantly less efficient in insect cells than the analogous step in mammalian cells, even though the cleavage sites of authentic and recombinant gl were shown to ... | 1992 | 1326809 |
| sequences within the feline immunodeficiency virus long terminal repeat that regulate gene expression and respond to activation by feline herpesvirus type 1. | we constructed a series of deletion mutants of feline immunodeficiency virus (fiv) long terminal repeat (ltr) to identify the regions that regulate gene expression and are responsive to trans-activation of fiv ltr by feline herpes-virus type 1 (fhv-1). we demonstrated that sequences between -124 and -79, and between -21 and -32 (relative to the cap site) are essential for gene expression of fiv in felis catus whole fetus 4 (fcwf-4) cells. further, we demonstrated that the sequence between -63 an ... | 1992 | 1326814 |
| phenotypic and genetic polymorphisms among human herpesvirus-6 isolates from north american infants. | fifteen human herpesvirus-6 (hhv-6) isolates from north american infants with primary infection manifest as febrile or roseola (exanthem subitum) like illnesses were characterized phenotypically on the basis of their in vitro growth in continuous t-cell lines and primary human mononuclear cells and by their reactivity with monoclonal antibodies. all isolates replicated efficiently in primary human cord blood mononuclear cells, but five distinct patterns of viral replication in human cells lines ... | 1992 | 1326815 |
| herpes simplex virus infections. | this review discusses current reports on herpes simplex virus infections as they relate to the use of laboratory testing, infections in the neonate, herpes simplex virus association with human immunodeficiency virus infection, and updating the current therapy and management of genital herpes. findings over the past year are important in the clinical management of patients with genital herpes. all health care workers who manage patients with genital herpes need to know the limitations of serologi ... | 1992 | 1327252 |
| epidemiological and biological study of acquired immunodeficiency syndrome-related lymphoma in the county of los angeles: preliminary results. | a population-based case control study of intermediate- and high-grade lymphoma in the county of los angeles, ca, was initiated in 1989. human immunodeficiency virus (hiv)-positive lymphoma patients are compared to hiv-negative lymphoma patients, to hiv-positive controls with acquired immunodeficiency syndrome but without lymphoma, and to hiv-positive asymptomatic individuals. the hiv-negative lymphoma cases are compared to neighborhood controls, who are matched in terms of age, sex, race/ethnici ... | 1992 | 1327509 |
| sequences of the bovine herpesvirus 1 homologue of herpes simplex virus type-1 alpha-trans-inducing factor (ul48). | a virion protein of herpes simplex virus type-1, called vmw65, alpha tif or vp16, interacts with cellular transcription factors to transactivate immediate early viral genes. we have cloned and determined the nucleotide sequence of the gene encoding the homologous protein in bovine herpesvirus 1 (bhv-1). the amino acid sequence of the bhv-1 protein is similar to that of alpha tif, except in the c-terminal one-third of the protein. since the ability of alpha tif to activate transcription is depend ... | 1992 | 1327963 |
| immunoelectron microscopy for rapid diagnosis of varicella-zoster virus in a complicated case of human t-cell lymphotropic virus type 1 infection. | rapid techniques for the detection of herpes simplex virus (hsv) and varicella-zoster virus (vzv) are needed for optimal therapeutic management. vzv infection poses a serious threat, especially to seriously ill patients, for instance, immunocompromised patients. we report a case of human t-cell lymphotropic virus type 1-positive leukemia complicated by atypical multidermatomal herpes zoster. viral culture and standard serological tests failed to prove vzv infection. herpesvirus infection was con ... | 1992 | 1328289 |
| several groups among human herpesvirus 6 strains can be distinguished by southern blotting and polymerase chain reaction. | 1992 | 1328291 | |
| acute encephalopathy following exanthem subitum caused by human herpesvirus-6. | 1992 | 1328421 | |
| virological and pathological features of mice infected with murine gamma-herpesvirus 68. | the primary infection of balb/c mice with murine herpesvirus 68 (mhv-68) was investigated. when the virus was introduced intranasally, the lung was the main tissue infected, the virus being associated with alveolar epithelium and mononuclear cells. a productive infection lasted for 10 days, after which viral dna could be detected by in situ hybridization up to 30 days after infection. at that time lymphoproliferative accumulations were also observed in the lung, with formation of germinal centre ... | 1992 | 1328491 |
| antigenic relationships among human herpesvirus-6 isolates. | human herpesvirus 6 (hhv-6) prototype isolate gs is a newly identified lymphotropic herpesvirus and several subsequent herpes isolates were recognized as hhv-6 by their hybridization to a hhv-6(gs) dna probe pzvh14. dna restriction analysis and in vitro tropism studies show that hhv-6 isolates can be divided into two groups, designated group a and group b. antigenic relationships among 15 hhv-6 isolates belonging to these two groups were examined using rabbit antibodies against hhv-6(gs) infecte ... | 1992 | 1328500 |
| detection of human herpesvirus-6 dna by polymerase chain reaction in serum or plasma. | human herpesvirus-6 (hhv-6) is a newly identified human pathogen. currently clinicians rely mainly on blood lymphocyte culture and serological tests to diagnose hhv-6 infection. the polymerase chain reaction (pcr) was carried out on the plasma or sera of patients to determine the value of pcr in the diagnosis of hhv-6 infection. a total of 30 patients entered the study; 10 were experiencing acute hhv-6 infections and 20 were healthy and served as controls. hhv-6 dna was detected by pcr in the se ... | 1992 | 1328512 |
| evidence for an alpha-herpesvirus indigenous to mountain gorillas. | sera from wild mountain gorillas were screened for antibodies reactive with primate alpha-herpesviruses. four of seven individuals tested (58%) were positive. in all four sera the highest titers were to hsv-2 followed by hsv-1 and sa8. immunoblot analysis confirmed a preferential reactivity with hsv-2 antigen. further analysis by competition elisa indicated that these gorillas had experienced infection with a virus antigenically similar but not identical to hsv-2. these results represent the fir ... | 1992 | 1328641 |
| herpes simplex virus type 1 dutpase mutants are attenuated for neurovirulence, neuroinvasiveness, and reactivation from latency. | herpes simplex virus type 1 (hsv-1) encodes a dutpase which has been shown to be dispensable for normal viral replication in cultured cells (s. j. caradonna and y. cheng, j. biol. chem. 256:9834-9837, 1981; f. b. fisher and v. g. preston, virology 148:190-197, 1986). however, the importance of this enzyme in vivo has not been determined. in this report, hsv-1 strain 17 syn+ and two isogenic engineered dutpase-negative mutants were characterized in the mouse model. both mutants replicated with wi ... | 1992 | 1328686 |
| [complications of acute respiratory tract infection (pneumonia, sinusitis) in young children associated with acute hhv 6 infection]. | the human herpesvirus-6 (hhv-6) causes exanthema subitum ("e.s."). "e.s." is characterized by fever, exanthem (rash), in many cases gastroenteritis, occasionally cerebral convulsions (but more frequently general cerebral irritability) and enlargement of all lymph nodes; usually there are mild catarrhal respiratory symptoms of the upper airways (ard). so-called "complications" of an ard (pneumonia, acute purulent otitis media, acute sinusitis) due to bacterial infections are very unusual as seque ... | 1992 | 1328758 |
| antiviral activity of a bile pigment, biliverdin, against human herpesvirus 6 (hhv-6) in vitro. | biliverdin (bv), a bile pigment, was examined for its antiviral activity against human herpesvirus-6 (hhv-6) in vitro. bv (10 micrograms/ml) markedly inhibited hhv-6 replication in mt-4 cells when the cells were treated during a virus adsorption period. its antiviral effect was weakened when cells were treated after adsorption. treatment of cells with bv (40 micrograms/ml) 3 hr after virus infection had no inhibitory effect on virus replication. virus replication was also significantly inhibited ... | 1992 | 1328826 |
| cell-protecting effect against herpes simplex virus-1 and cellular metabolism of 9-(2-phosphonylmethoxyethyl)adenine in hela s3 cells. | 9-(2-phosphonylmethoxyethyl)adenine (pmea) is a selective and potent inhibitor of retrovirus and herpesvirus replication in vitro and in vivo. in cell culture studies, pretreatment of hela s3 cells with pmea before infection enhanced its antiviral potency by almost 10-fold, compared with treatment of the cells only after viral infection. to elucidate the basis for this observation, the uptake, metabolism, and retention of pmea metabolites were examined in uninfected and herpes simplex virus type ... | 1992 | 1328849 |
| comparison of immunocytology to tissue culture for diagnosis of presumed herpesvirus dendritic epithelial keratitis. | the objective of this study is to prospectively compare the sensitivity and specificity of immunodetection of herpes simplex virus (hsv) in impression cytology specimens obtained directly from presumed herpesvirus dendritic epithelial keratitis with virus isolation by tissue culture of cells scraped from the same lesion. | 1992 | 1328982 |
| primary human herpesvirus-6 infection (exanthem subitum) in the newborn. | 1992 | 1329004 | |
| [infection by human herpesvirus type 6 (hhv-6) and renal transplantation]. | human herpes virus type 6 (hhv-6) infection was serologically investigated in renal transplant recipients. before transplantation, 75.5% of patients was seropositive for hhv-6 and no correlation with age, sex and time on dialysis was found. during the first month after transplantation 66% of patients showed a variation in serological status against hhv-6 (seroconversion or fourfold increase of antibody titer). all patients who seroconverted had received the kidney from a hhv-6 seropositive donor ... | 1992 | 1329241 |
| equine herpesvirus 5: comparisons with ehv2 (equine cytomegalovirus), cloning, and mapping of a new equine herpesvirus with a novel genome structure. | a new equine herpesvirus, provisionally designated equine herpesvirus 5 (ehv5; browning and studdert (1987) j. gen. virol. 68, 1441-1447), was examined for the degree of genomic difference from equine herpesvirus 2 (ehv2) by southern hybridizations. ehv5 and ehv2 whole genomic dna probes were highly specific for homologous dna only, indicating that significant genomic difference exists between the two viruses. restriction endonuclease analysis of ehv5 strain 2-141 (ehv5.2-141) revealed that the ... | 1992 | 1329316 |
| adenovirus containing a deletion of the early region 2a gene allows growth of adeno-associated virus with decreased efficiency. | efficient growth of adeno-associated virus (aav) requires helper functions provided by a coinfecting adenovirus or herpesvirus. earlier studies using adenoviruses having temperature-sensitive lesions in the early region 2a gene (e2a) produced contradictory evidence regarding the role of the e2a 72-kda dna-binding protein (dbp) in allowing efficient aav growth. these disparate results may reflect varying levels of residual function in the temperature-sensitive dbp. we examined this issue using an ... | 1992 | 1329332 |
| characterization of human herpesvirus-6(u1102) and (gs) gp112 and identification of the z29-specified homolog. | monoclonal antibody 2d10 (mab 2d10) raised toward human herpesvirus-6(u1102) [hhv6(u1102)] immunoprecipitated three glycosylated peptides, m(r) 112,000, 64,000, and 58,000, designated as gp112 from u1102-infected lymphocytes. pulse-chase experiments suggest that the m(r) 64,000 and 58,000 polypeptides are very likely generated by post-translational cleavage of the m(r) 112,000 polypeptide. mab 2d10 neutralized virion infectivity in the presence of complement, suggesting that gp112 is located in ... | 1992 | 1329335 |
| homology of the envelope glycoprotein b of human herpesvirus-6 and cytomegalovirus. | the envelope glycoprotein b (gb) coding sequences of two strains of human herpesvirus-6 (hhv6 gs and z29) were determined by sequencing a 2.5-kb open reading frame adjacent to the dna polymerase sequence. the deduced primary translation product is 830 amino acids in length and is 96% conserved between the two divergent strains with no localized hypervariability noted. it contains the expected signal and transmembrane sequence motifs as well as a putative site of protease cleavage. there was 39% ... | 1992 | 1329336 |
| identification and expression of the ul1 gene product of equine herpesvirus 1. | sequences encoding the ul1 gene of equine herpesvirus type 1 (ehv-1) are conserved in the genome of ehv-1 defective interfering particles (dips) that mediate oncogenic transformation and persistent infection. the ul1 protein was identified by in vitro transcription/translation and hybrid-arrest translation analyses which employed a ul1/pgem-3z construct designated pgeml1. sds-page analyses of in vitro translation products synthesized from ul1-specific rna revealed that the ul1 orf encodes a 30 k ... | 1992 | 1329372 |
| the acyclic nucleoside analogue penciclovir is a potent inhibitor of equine herpesvirus type 1 (ehv-1) in tissue culture and in a murine model. | equine herpesvirus type 1 (ehv-1) was sensitive to the nucleoside analogue penciclovir (pcv) when tested in tissue culture; the ed50 was 1.6 micrograms/ml. drug-resistant mutants were selected which were found to be tk-defective and approx. 45-fold less sensitive to pcv compared with the parental strain. pcv was compared with the phosphonyl derivative, hpmpa in mice infected with ehv-1. both drugs were shown to be effective in vivo, limiting wild-type virus replication in respiratory tissues, an ... | 1992 | 1329646 |
| differentiation of oncogenic and nononcogenic strains of marek's disease virus type 1 by using polymerase chain reaction dna amplification. | differentiation of oncogenic and nononcogenic strains of marek's disease virus type 1 (mdv1) was attempted by polymerase chain reaction (pcr) using the primers chosen from the sequence within the long inverted repeats of mdv1 dna. pcr of the dnas extracted from oncogenic-strain-infected cells and marek's disease tumor cell lines produced a major product containing two or three copies of 132-base-pair (bp) repeat units, whereas pcrs of the dnas extracted from nononcogenic-strain-infected cells yi ... | 1992 | 1329711 |
| t cell recognition of epstein-barr virus associated lymphomas. | epstein-barr virus, a lymphotropic herpesvirus of humans, has potent b cell growth transforming activity yet persists in the lymphoid tissues of most individuals as a lifelong asymptomatic infection. virus induced b cell growth transformation in vitro is associated with the expression of a limited set of viral genes encoding six nuclear antigens (ebna 1, 2, 3a, 3b, 3c and lp) and two latent membrane proteins (lmp 1, 2). healthy virus carriers possess strong ebv specific ctl memory that can be re ... | 1992 | 1330300 |
| herpesvirus serology, aberrant specific immunoglobulin g2 and g3 subclass patterns and gm allotypes in individuals with low levels of igg3. | one objective of this study was to determine whether igg3-deficient individuals have an increased frequency of reactivated herpesvirus infections. serum titres to epstein-barr virus (ebv) and human herpesvirus-6 were examined in 10 healthy and in 10 symptomatic persons with serum igg3 < 0.1 g/l. atypical titres were found in 16% of the igg3-deficient individuals. reactivations of these viruses thus do not seem common in igg3 deficiency. antigen-specific igg responses were also determined. a lowe ... | 1992 | 1330389 |
| immunosuppression in goats by dexamethasone and cyclophosphamide. | the influence of dexamethasone and cyclophosphamide on the goat immune system was investigated. seven goats, with a previous contact with caprine herpesvirus type 1 (chv-1), were used. all had been vaccinated with live mycobacterium paratuberculosis vaccine. six goats were injected intravenously (i.v.) with dexamethasone daily for 5 days (2.5-4 mg/kg bw per day). three also received 25 mg/kg bw of cyclophosphamide on day 0. the seventh goat was not treated. dexamethasone alone caused depression, ... | 1992 | 1330422 |
| effects in calves of mixed infections with bovine viral diarrhea virus and several other bovine viruses. | the objective of this study was to verify whether a mixed infection in calves with bovine viral diarrhea virus (bvdv) and other bovine viruses, such as bovid herpesvirus-4 (bhv-4), parainfluenza-3 (pi-3) and infectious bovine rhinotracheitis (ibr) virus, would influence the pathogenesis of the bvdv infection sufficiently to result in the typical form of mucosal disease being produced. accordingly, two experiments were undertaken. in one experiment calves were first infected with bvdv and subsequ ... | 1992 | 1330424 |
| viral co-infections in human papillomavirus-associated anogenital lesions according to the serostatus for the human immunodeficiency virus. | in hiv-infected men, human papillomavirus (hpv) infection is strongly linked with the development of anogenital lesions but is not a sufficient factor to explain the neoplastic transformation of such lesions. we investigated the association between hpv and herpesvirus infections in penile and anal lesions from 54 hiv-seronegative and 54 hiv-seropositive men by means of colposcopy, histopathology and in situ hybridization. our patients showed condyloma acuminata (39%), papular warts (35%) and mac ... | 1992 | 1330931 |
| comparison of three immunoassays for the detection of anti-hhv6. | sera from 96 blood donors were tested for antibody to human herpesvirus 6 by indirect immunofluorescence (if), circle immunoassay (cia) and competitive radioimmunoassay (ria). the correlation between the three assays was good but the cia and competitive ria were more sensitive for the detection of hhv6 antibody than indirect if. the crossreaction of hhv6 antibody with that to the other human herpesviruses was also studied in this blood donor group. no correlation was found between antibody to hu ... | 1992 | 1331142 |
| generation of macaque b lymphoblastoid cell lines with simian epstein-barr-like viruses: transformation procedure, characterization of the cell lines and occurrence of simian foamy virus. | two simian epstein-barr-like viruses, a rhesus epstein-barr virus and herpesvirus papio, were used to transform b cells from rhesus or cynomolgus macaques. the resulting cell lines exhibited predominantly a b lymphocyte phenotype and expressed epstein-barr virus antigens. the majority of b lymphoblastoid cell lines from macaques, which were seropositive for simian foamy virus, developed giant cells in culture. the cytopathic agent was identified as a foamy virus and was transmissible to human em ... | 1992 | 1331148 |
| rapid detection of equine herpesvirus type-1 antigens in nasal swab specimens using an antigen capture enzyme-linked immunosorbent assay. | an antigen capture enzyme-linked immunosorbent assay (elisa) was developed for the detection of equine herpesvirus type-1 (ehv-1) antigens in nasal swab specimens. the test was designed as a solid phase, amplified sandwich assay in which an ehv-1 specific monoclonal antibody was used to capture virus antigen and polyclonal antisera used to detect antigen bound to the test plates. eight monoclonal antibodies were tested for their ability to capture virus antigen and one was selected for routine u ... | 1992 | 1331153 |
| blocking elisa for distinguishing infectious bovine rhinotracheitis virus (ibrv)-infected animals from those vaccinated with a gene-deleted marker vaccine. | a sensitive and specific blocking enzyme-linked immunosorbent assay (elisa) was developed to distinguish infectious bovine rhinotracheitis virus (ibrv)-infected animals from those immunized with a glycoprotein giii deletion mutant, ibrv(ng)dltkdlgiii. for this elisa, undiluted test sera are used to block the binding of an anti-ibrv giii monoclonal antibody (mabgiii)-horseradish peroxidase (hrpo) conjugate to giii antigen. tmb substrate is used for color development. negative s/n values (defined ... | 1992 | 1331160 |