Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| surveillance of pathogens causing gastroenteritis and characterization of norovirus and sapovirus strains in shenzhen, china, during 2011. | viral gastroenteritis is one of the most common diseases in humans, and it is primarily caused by rotaviruses (rvs), astroviruses (astvs), adenoviruses (advs), noroviruses (novs), and sapoviruses (savs). in this study, we determined the distribution of viral gastroenteritis and human calicivirus (hucvs) in acute gastroenteritis patients in shenzhen, china, during 2011. real-time rt-pcr was used to detect norovirus (nov), group a rotavirus (rv), adenovirus (adv), and astrovirus (astv). from a tot ... | 2014 | 24610551 |
| diarrhoea-related hospitalizations in children before and after implementation of monovalent rotavirus vaccination in mexico. | to assess, by socioeconomic setting, the effect of nationwide vaccination against species a rotavirus (rva) on childhood diarrhoea-related hospitalizations in mexico. | 2014 | 24623905 |
| fluorescence in situ hybridization investigation of potentially pathogenic bacteria involved in neonatal porcine diarrhea. | neonatal diarrhea is a multifactorial condition commonly present on pig farms and leads to economic losses due to increased morbidity and mortality of piglets. immature immune system and lack of fully established microbiota at birth predispose neonatal piglets to infection with enteric pathogens. the microorganisms that for decades have been associated with enteritis and diarrhea in suckling piglets are: rotavirus a, coronavirus, enterotoxigenic escherichia coli (etec), clostridium perfringens t ... | 2014 | 24628856 |
| evaluation of a multiplex pcr assay for simultaneous detection of bacterial and viral enteropathogens in stool samples of paediatric patients. | we evaluated a multiplex pcr assay, the seeplex diarrhoea ace detection, that simultaneously detects 15 enteric pathogens, including salmonella spp., shigella spp., vibrio spp., toxin b producer clostridium difficile, campylobacter spp., clostridium perfringens, yersinia enterocolitica, aeromonas spp., escherichia coli o157:h7, verocytotoxin-producing escherichia coli, adenovirus, group a rotavirus, norovirus gi and gii, and astrovirus. we compared this assay with clinical methods routinely used ... | 2014 | 24656922 |
| predominance of rotavirus g8 genotype in hospitalized children with acute gastroenteritis in yasuj, iran. | rotavirus infection is the most common cause of gastroenteritis among children worldwide. in this study, we were evaluated the occurrence of rotavirus infection and genotypes causing gastroenteritis among children under 5 years in yasuj, iran. | 2014 | 24668711 |
| full-genome sequence of a rare human g3p[9] rotavirus strain. | this is a report of the complete genomic sequence of a rare rotavirus group a g3-p[9]-i2-r2-c2-m2-a3-n2-t1-e2-h3 strain designated rva/human-wt/usa/12us1134/2012/g3p[9]. | 2014 | 24675848 |
| sequence and structural analyses of nsp4 proteins from human group a rotavirus strains detected in tunisia. | the nsp4 protein of group a rotavirus (rva) has been recognized as a viral enterotoxin and plays important roles in viral pathogenesis and morphogenesis. domains involved in structural and functional interactions have been proposed mainly based on the simian sa11 strain. | 2014 | 24679587 |
| virucidal effect of commercially available disinfectants on equine group a rotavirus. | although many disinfectants are commercially available in the veterinary field, information on the virucidal effects of disinfectants against equine group a rotavirus (rva) is limited. we evaluated the performance of commercially available disinfectants against equine rva. chlorine- and iodine-based disinfectants showed virucidal effects, but these were reduced by the presence of organic matter. glutaraldehyde had a virucidal effect regardless of the presence of organic matter, but the effect wa ... | 2014 | 24681569 |
| evaluation of the rapid test for human rotavirus a in thai children with acute gastroenteritis. | rapid tests are widely used to detect rotavirus a; however, pediatricians are concerned whether the rapid test can still accurately detect the virus. therefore, this study evaluated the performance of the rotavirus rapid test by comparing it to the one-step rt-pcr method. | 2014 | 24697131 |
| full-length genomic analysis of porcine rotavirus strains isolated from pigs with diarrhea in northern italy. | group a rotaviruses (rva) cause acute dehydrating diarrhea in young of man and many animal species, including pigs. swine rva has an important economic impact on the farming industry, and pigs represent a potential reservoir for zoonotic transmission of rva to humans. to investigate the genetic diversity of porcine rva strains in italy and identify their possible zoonotic characteristics, 25 rva-positive feces were collected from diarrheic pigs in northern italy, in 2009-2010; all viral strains ... | 2014 | 24704759 |
| rotavirus infection among sudanese children younger than 5 years of age: a cross sectional hospital-based study. | in sudan, rotavirus has been one of the important causative agents of diarrhea among children. rotavirus a is well known as the leading cause of diarrhea in young children worldwide. it was estimated to account for 41% of hospitalized cases of acute gastroenteritis among children in sub-saharan africa. this study aimed to determine the prevalence and the common clinical presentations of rotavirus a infection among sudanese children with gastroenteritis seeking management in hospitals. | 2013 | 24711878 |
| a two-tube multiplex reverse transcription pcr assay for simultaneous detection of viral and bacterial pathogens of infectious diarrhea. | diarrhea caused by viral and bacterial infections is a major health problem in developing countries. the purpose of this study is to develop a two-tube multiplex pcr assay using automatic electrophoresis for simultaneous detection of 13 diarrhea-causative viruses or bacteria, with an intended application in provincial centers for diseases control and prevention, china. the assay was designed to detect rotavirus a, norovirus genogroups gi and gii, human astrovirus, enteric adenoviruses, and human ... | 2014 | 24711998 |
| [serological survey of antibodies against viral diseases of public health interest in llamas (lama glama) from jujuy province, argentina]. | llama population from argentina is mainly concentrated in the andean puna, jujuy. llamas represent an important economic resource for the andean communities. the aim of this study was to investigate the prevalence of antibodies against viral antigens associated to viral diseases of economic impact (neonatal diarrhea, reproductive and respiratory syndromes). a total of 349 serum samples from adult llamas were analyzed. the obtained antibody prevalence was 100 % for rotavirus a and 70 % for bovine ... | 2014 | 24721276 |
| identification of novel bovine group a rotavirus g15p[14] strain from epizootic diarrhea of adult cows by de novo sequencing using a next-generation sequencer. | there are few reports describing diarrhea of adult cattle caused by group a rotaviruses. here, we report the identification of a novel bovine group a rotavirus from diarrhea of adult cows. a group a rotavirus was detected from an epizootic outbreak of diarrhea in adult cows with a decrease in milk production in japan in 2013. the comprehensive genomic analyses from fecal samples by viral metagenomics using a next-generation sequencer revealed that it had an unreported genotype combination g15p[1 ... | 2014 | 24725447 |
| the first caprine rotavirus detected in argentina displays genomic features resembling virus strains infecting members of the bovidae and camelidae. | rotavirus group a (rva) is a major cause of diarrhea in humans and young animals including small ruminants. the purpose of this study was to identify rva in dairy goat kids, and to characterize the complete genomic constellation and genetic relatedness with other rva strains. four out of twenty fecal samples from diarrheic and non-diarrheic goat kids were positive for rva by elisa. a representative sample was selected for further genome analyses. the rva strain rva/goat-wt/arg/0040/2011/g8p[1] d ... | 2014 | 24742949 |
| [prognosis of rotavirus infection in children]. | the aim of the research was to develop the method for predicting the course of rotavirus gastroenteritis in children.under the supervision were 3607 children aged from 9 days to 5 years with the diagnosis of "acute gastroenteritis" and "acute gastroenterocolitis". the diagnosis of rotavirus infection was on the basis of a set of clinical, epidemiological data and the results of para-clinical and bacteriological studies and data of detection of rotavirus antigen strain. genotyping of rotavirus gr ... | 2014 | 24743127 |
| molecular analysis of non structural rotavirus group a enterotoxin gene of bovine origin from india. | the rotavirus enterotoxin nsp4 (nonstructural protein 4), plays a pivotal role in viral morphogenesis as well as pathogenesis. in this study, the nsp4 gene of rotavirus group a (rva) isolates of bovine origin isolated in several states of india from 2008 to 2011 were characterized. the complete open reading frame of 23 rva strains were sequenced and analyzed phylogenetically. genotype e1 was detected for the first time in bovines from india, in addition to the more common bovine genotype e2. seq ... | 2014 | 24747605 |
| distinct evolutionary origins of g12p[8] and g12p[9] group a rotavirus strains circulating in brazil. | g12 group a rotavirus (rva) are currently recognized as a globally emerging genotype and have been described in combination with several p-types. in brazil, g12 rva strains have been described in the southern (2003) and northern (2008-2010) regions, in combination with the p[9] and p[6] genotype, respectively. to date, few complete genomes of g12 rva strains have been described (none from brazilian strains), considering g12p[9] genotype just one strain, rva/human-tc/tha/t152/1998/g12p[9], has th ... | 2014 | 24747965 |
| the effect of new neonatal porcine diarrhoea syndrome (nnpds) on average daily gain and mortality in 4 danish pig herds. | the study evaluated the effect of new neonatal porcine diarrhoea syndrome (nnpds) on average daily gain (adg) and mortality and described the clinical manifestations in four herds suffering from the syndrome. nnpds is a diarrhoeic syndrome affecting piglets within the first week of life, which is not caused by enterotoxigenic escherichia coli (etec), clostridium perfringens (c. perfringens) type a/c, clostridium difficile (c. difficile), rotavirus a, coronavirus, cystoisospora suis, strongyloide ... | 2014 | 24755093 |
| occurrence and characterization of rotavirus a in broilers, layers, and broiler breeders from brazilian poultry farms. | rotaviruses are a major cause of diarrhea in humans and animals, including several mammalian and avian species. using different pcr protocols, we report the occurrence of rotavirus a in 21 (53.84%; 21/39) from 39 fecal pool samples of broilers, layers, and broiler breeders from brazilian avian farms. we typed the g5, g8, g11, g19, and p[31] genotypes. | 2014 | 24758129 |
| assessment of gastroenteric viruses from wastewater directly discharged into uruguay river, uruguay. | the aim of this study was to assess the viral contamination of group a rotavirus (rva), norovirus (nov), and human astrovirus (hastv) in sewage directly discharged into uruguay river and to characterize rva genotypes circulating in uruguay. for this purpose, sewage samples (n = 96) were collected biweekly from march 2011 to february 2012 in four uruguayan cities: bella unión, salto, paysandú, and fray bentos. each sample was concentrated by ultracentrifugation method. qualitative and quantitativ ... | 2014 | 24777819 |
| screening of viral pathogens from pediatric ileal tissue samples after vaccination. | in 2010, researchers reported that the two us-licensed rotavirus vaccines contained dna or dna fragments from porcine circovirus (pcv). although pcv, a common virus among pigs, is not thought to cause illness in humans, these findings raised several safety concerns. in this study, we sought to determine whether viruses, including pcv, could be detected in ileal tissue samples of children vaccinated with one of the two rotavirus vaccines. a broad spectrum, novel dna detection technology, the lawr ... | 2014 | 24778651 |
| rotavirus inhibits ifn-induced stat nuclear translocation by a mechanism that acts after stat binding to importin-α. | the importance of innate immunity to rotaviruses is exemplified by the range of strategies evolved by rotaviruses to interfere with the ifn response. we showed previously that rotaviruses block gene expression induced by type i and ii ifns, through a mechanism allowing activation of signal transducer and activator of transcription (stat) 1 and stat2 but preventing their nuclear accumulation. this normally occurs through activated stat1/2 dimerization, enabling an interaction with importin α5 tha ... | 2014 | 24814927 |
| assessment of water quality in a border region between the atlantic forest and an urbanised area in rio de janeiro, brazil. | the preservation of water resources is one of the goals of the designation of parks that act as natural reservoirs. in order to assess the impact of the presence of humans in an environmental preservation area bordering urban areas, the presence of four pathogenic enteric viruses [group a rotavirus (rv-a), norovirus (nov), human adenoviruses (hadv), and hepatitis a virus (hav)], as well as the physico-chemical parameters, and escherichia coli levels were assessed in riverine water samples. from ... | 2014 | 24818567 |
| molecular detection of human noroviruses in influent and effluent samples from two biological sewage treatment plants in the region of monastir, tunisia. | noroviruses (novs) are responsible for numerous cases of waterborne and foodborne gastroenteritis every year. they are released in the sewage and their detection in this environment can reflect the epidemiology of the viral strains circulating in the community. a three-year (2007-2010) survey was conducted in order to evaluate the presence of human novs using rt-pcr in 518 sewage samples collected at the entrance and exit of two biological sewage treatment plants located in monastir region, tuni ... | 2014 | 24818568 |
| [investigation of seven different rna viruses associated with gastroenteritis in children under five years old]. | viruses are the most frequently detected etiologic agents of gastroenteritis seen in small children. in addition to classical gastroenteritis viruses namely rotavirus, norovirus, adenovirus type 40/41, astrovirus and sapovirus, some novel picornaviruses (aichi virus, parechovirus, enterovirus) that have been identified in parallel to the developments in molecular diagnostic methods, thought to be associated with diarrhea in humans. however, the data are not enough to prove their actual roles in ... | 2014 | 24819261 |
| relative abundance and treatment reduction of viruses during wastewater treatment processes--identification of potential viral indicators. | waterborne pathogenic viruses discharged from wastewater treatment plants (wwtps) pose potential public health risks. in the present study, we investigated the relative abundance, occurrence, and reduction of eleven different viruses at two wwtps in southern arizona over a 12-month period, from august 2011 to july 2012. influent and effluent samples from the two wwtps were collected monthly. viruses were concentrated using an electronegative filter method and quantified using taqman-based quanti ... | 2014 | 24836386 |
| alterations in oxidant/antioxidant balance, high-mobility group box 1 protein and acute phase response in cross-bred suckling piglets suffering from rotaviral enteritis. | rotaviral enteritis has emerged as a major cause of morbidity and mortality in piglets during their post-natal life. the present study was carried out to examine high-mobility group box 1 (hmgb1) protein, acute phase response and oxidative stress indices in the serum of suckling piglets suffering from enteritis with or without association of porcine group a rotavirus infection. the present investigation utilized 23 clinical cases with signs of acute enteritis and 12 more healthy piglets of a sim ... | 2014 | 24848720 |
| gastroenteritis outbreaks caused by a ds-1-like g1p[8] rotavirus strain, japan, 2012-2013. | rotavirus a (rva) genotype g1p[8], a hallmark of the wa-like strain, typically contains only genotype 1 genes. however, an unusual rva g1p[8] with genotype 2 genes was recently detected in japan. we determined the complete genomic constellation of this rva. our findings suggest that mixed rvas may be more competitive than once thought. | 2014 | 24856174 |
| comparison of pathogenicities and nucleotide changes between porcine and bovine reassortant rotavirus strains possessing the same genotype constellation in piglets and calves. | although reassortment is one of the most important characteristics of group a rotavirus (rva) evolution, the host range restriction and/or virulence of reassortant rvas remain largely unknown. the porcine 174-1 strain isolated from a diarrheic piglet was identified as a reassortant strain, harboring the same genotype constellation as the previously characterized bovine strain kj56-1. owing to its same genotype constellation, the pathogenicity of the porcine strain 174-1 in piglets and calves was ... | 2014 | 24861840 |
| surveillance of group a rotavirus in buenos aires 2008-2011, long lasting circulation of g2p[4] strains possibly linked to massive monovalent vaccination in the region. | group a rotaviruses (rva) are the most frequent single etiological agents of severe diarrhea in infants. since 2006 rva vaccines have been introduced in national schedules of middle and high income countries with substantial declines in rotavirus associated disease burden. however, surveillance must be maintained to, eventually, detect emerging types or variants selected by the new pressure imposed by vaccination. | 2014 | 24875137 |
| predicted structure and domain organization of rotavirus capping enzyme and innate immune antagonist vp3. | rotaviruses and orbiviruses are nonturreted reoviridae members. the rotavirus vp3 protein is a multifunctional capping enzyme and antagonist of the interferon-induced cellular oligoadenylate synthetase-rnase l pathway. despite mediating important processes, vp3 is the sole protein component of the rotavirus virion whose structure remains unknown. in the current study, we used sequence alignment and homology modeling to identify features common to nonturreted reoviridae capping enzymes and to pre ... | 2014 | 24899176 |
| [whole genome analysis of human group a rotavirus g9p[8] strains in hebei lulong region, 2009-2011]. | abstract:this study aims to investigate the genetic characteristics of group a rotavirus (garv) g9p[8] strains from infantile diarrhea samples in hebei lulong region from 2009 to 2011. we randomly selected five garv g9p[8] strains in hebei lulong region from 2009 to 2011, amplified the 11 gene fragments of garvs by rt-pcr, and analyz their full-genome sequences by homology and phylogenetic analysis with dnastar and mega. the nucleotide homology between strains ll11131077 and ll11131083 in 2011 w ... | 2014 | 24923163 |
| complete genome analysis of a rare group a rotavirus, g11p[25], isolated from a child in mumbai, india, reveals interspecies transmission and reassortment with human rotavirus strains. | hospital-based rotavirus surveillance was carried out in mumbai during 2005-2009. an isolate (b08299) with a rare genotype combination (g11p[25]) was detected. the present study was undertaken to characterize the complete genome of the isolate. b08299 exhibited a g11-p[25]-i12-r1-c1-m1-a1-n1-t1-e1-h1 genotype constellation. phylogenetic analysis of the 11 gene segments of b08299 revealed that the vp2 and nsp5 genes of b08299 had a human origin, while the vp6 gene represented an i12 genotype of o ... | 2014 | 24951672 |
| rotavirus surveillance in kisangani, the democratic republic of the congo, reveals a high number of unusual genotypes and gene segments of animal origin in non-vaccinated symptomatic children. | group a rotavirus (rva) infections form a major public health problem, especially in low-income countries like the democratic republic of the congo (cod). however, limited data on rva diversity is available from sub-saharan africa in general and the cod in particular. therefore, the first aim of this study was to determine the genetic diversity of 99 rvas detected during 2007-2010 in kisangani, cod. the predominant g-type was g1 (39%) and the most predominant p-type was p[6] (53%). a total of ei ... | 2014 | 24968018 |
| gastroenteric virus dissemination and influence of rainfall events in urban beaches in brazil. | this study was conducted to assess rotavirus a (rv-a), genogroup ii (gii) norovirus (nov), and human adenovirus (hadv) dissemination in recreational water in an urban beach located in the city of rio de janeiro and their persistence during rainfall events. | 2014 | 24980661 |
| host-pathogen co-evolution and glycan interactions. | noroviruses and rotavirus a bind to polymorphic glycans of the histo-blood group type (hbgas). norovirus strains that bind to hbgas can collectively infect all humans but each strain only infects a subgroup of the population, suggesting a past co-evolution that led to a trade-off where the human population is partly protected whilst the virus circulation is maintained. we termed 'herd innate protection' the host species partial protection provided by the hbgas polymorphism. given its recent emer ... | 2014 | 25000207 |
| human group a rotavirus infections in children in denmark: detection of reassortant g9 strains and zoonotic p[14] strains. | one of the leading causes of severe childhood gastroenteritis are group a rotaviruses, and they have been found to be associated with ∼40% of the annual gastroenteritis-associated hospitalizations in young danish children <5years of age (fischer et al., 2011). in this study, we investigated the diversity of rotavirus strains circulating among young children <5years of age, presenting with gastroenteritis disease either at the general practitioner or in the hospital, during the period 2009-2013. ... | 2014 | 25038295 |
| simultaneous concentration of bovine viruses and agricultural zoonotic bacteria from water using sodocalcic glass wool filters. | infiltration and runoff from manured agricultural fields can result in livestock pathogens reaching groundwater and surface waters. here, we measured the effectiveness of glass wool filters to simultaneously concentrate enteric viruses and bacteria of bovine origin from water. the recovery efficiencies were determined for bovine viral diarrhea virus types 1 and 2, bovine rotavirus group a, bovine coronavirus, poliovirus sabin iii, toxigenic escherichia coli ,and campylobacter jejuni seeded into ... | 2014 | 25059211 |
| acute gastroenteritis and enteric viruses in hospitalised children in southern brazil: aetiology, seasonality and clinical outcomes. | viral acute gastroenteritis (ag) is a significant cause of hospitalisation in children younger than five years. group a rotavirus (rva) is responsible for 30% of these cases. following the introduction of rva immunisation in brazil in 2006, a decreased circulation of this virus has been observed. however, ag remains an important cause of hospitalisation of paediatric patients and only limited data are available regarding the role of other enteric viruses in these cases. we conducted a prospectiv ... | 2014 | 25075782 |
| group a rotavirus gastroenteritis in older children and adults at a hospital in southern india. | there is limited data on the spectrum and prevalence of rotavirus genotypes in older children and adults in asia. this pilot study conducted between november 2012 and april 2013 tested for rotavirus in older children (>12 years of age), and adults with gastroenteritis from southern india. stool samples from patients who were hospitalized or attended out-patient units with diarrhea were screened for rotavirus using premier™ rotaclone(®). confirmatory testing was by another antigen detection sandw ... | 2014 | 25091677 |
| characterisation of rotavirus strains identified in adolescents and adults with acute gastroenteritis highlights circulation of non-typeable strains: 2008-2012. | group-a rotavirus (rv) is the main causative agent of acute gastroenteritis in children < 5 years of age. its role as a pathogen in adults needs to be monitored. the aim of this study was to characterise the group-a rv strains that cause infections of acute gastroenteritis in adolescents and adults and determine the temporal variations in the circulating strains during 2008-2012 in continuation of an earlier study conducted in 2004-2007, in pune, india. | 2014 | 25091684 |
| molecular epidemiology of rotavirus in children and animals and characterization of an unusual g10p[15] strain associated with bovine diarrhea in south india. | rotaviruses are enteric pathogens causing acute, watery, dehydrating diarrhea in various host species, including birds and mammals. this study collected data on the disease burden and strain prevalence of group a rotavirus in animals and humans in vellore and investigated interspecies transmission by comparison of circulating genotypes. stool samples from children aged less than 5 years, admitted to the hospital between january 2003 and may 2006 for diarrhea and diarrheal samples from animals th ... | 2014 | 25091687 |
| whole genomic analysis of human g12p[6] and g12p[8] rotavirus strains that have emerged in kenya: identification of porcine-like nsp4 genes. | g12 rotaviruses are globally emerging rotavirus strains causing severe childhood diarrhea. however, the whole genomes of only a few g12 strains have been fully sequenced and analyzed, of which only one g12p[4] and one g12p[6] are from africa. in this study, we sequenced and characterized the complete genomes of three g12 strains (rva/human-tc/ken/kdh633/2010/g12p[6], rva/human-tc/ken/kdh651/2010/g12p[8], and rva/human-tc/ken/kdh684/2010/g12p[6]) identified in three stool specimens from children ... | 2014 | 25111611 |
| metagenomic identification of novel enteric viruses in urban wild rats and genome characterization of a group a rotavirus. | rats are known as reservoirs and vectors for several zoonotic pathogens. however, information on the viruses shed by urban wild rats that could pose a zoonotic risk to human health is scare. here, intestinal contents from 20 wild norway rats (rattus norvegicus) collected in the city of berlin, germany, were subjected to metagenomic analysis of viral nucleic acids. the determined faecal viromes of rats consisted of a variety of known and unknown viruses, and were highly variable among the individ ... | 2014 | 25121550 |
| genetic diversity of porcine group a rotavirus strains in the uk. | rotavirus is endemic in pig farms where it causes a loss in production. this study is the first to characterise porcine rotavirus circulating in uk pigs. samples from diarrheic pigs with rotavirus enteritis obtained between 2010 and 2012 were genotyped in order to determine the diversity of group a rotavirus (garv) in uk pigs. a wide range of rotavirus genotypes were identified in uk pigs: six g types (vp7); g2, g3, g4, g5, g9 and g11 and six p types (vp4); p[6], p[7], p[8], p[13], p[23], and p[ ... | 2014 | 25123085 |
| genomic characterization of a rotavirus g8p[1] detected in a child with diarrhea reveal direct animal-to-human transmission. | group a rotavirus is a major cause of severe gastroenteritis in children and young animals. during a retrospective analysis of samples collected from paraguayan children under 5 years old with diarrhea, and previously negative for rotavirus and norovirus, we detected the presence of bovine rotavirus sequences by viral metagenomics. nucleic acid was extracted direct from stool sample and determined to be g8p[1]. the genomic analyzes revealed that the strain presents an artiodactyl-like genome (g8 ... | 2014 | 25169054 |
| prevalence of rotavirus (garv) and coronavirus (bcov) associated with neonatal diarrhea in calves in western algeria. | to study the prevalence of bovine group a rotavirus (garv) and bovine coronavirus (bcov) in diarrheic feces from calves and the sensitive's parameters such as age group and sex. | 2014 | 25183104 |
| molecular surveillance of rotavirus infection in bangui, central african republic, october 2011-september 2013. | the world health organization (who) recommends the introduction of rotavirus vaccine in the immunization program of all countries. in the central african republic (car), sentinel surveillance for rotavirus gastroenteritis was established in 2011 by the ministry of health, with the support of the surveillance en afrique centrale project (survac). the purpose of this study was to assess the burden of rotavirus gastroenteritis and to identify rotavirus strains circulating in car before the introduc ... | 2014 | 25193563 |
| rapid detection and high occurrence of porcine rotavirus a, b, and c by rt-qpcr in diagnostic samples. | rotaviruses are important cause of diarrhea in animals, including humans. currently, rotavirus species a, b, c, e, and h (rva-rvc, rve, and rvh) have been identified in pigs. traditionally, rva has been considered the primary cause of diarrhea in pigs, and rvb and rvc had been described sporadically in pigs until recently. qualitative porcine rva, rvb, and rvc rt-pcr (rt-qpcr) assays were designed and 7508 porcine diarrheic samples, submitted to university of minnesota, were tested to estimate t ... | 2014 | 25194889 |
| rotavirus genotypes in belarus, 2008-2012. | this study describes group a rotavirus (rva) genotype prevalence in belarus from 2008 to 2012. in 2008, data from 3 sites in belarus (brest, mogilev, minsk) indicated that g4p[8] was the predominant genotype. data from minsk (2008-2012) showed that g4p[8] was the predominant rva genotype in all years except in 2011 when g3p[8] was most frequently detected. other rva genotypes common in europe (g1p[8], g2p[4]) were detected each year of the study. this study reveals the dominance of genotype g4p[ ... | 2014 | 25218086 |
| noroviruses associated with outbreaks of acute gastroenteritis in the state of rio grande do sul, brazil, 2004-2011. | acute gastroenteritis norovirus (nov) in a country of continental dimensions like brazil has resulted in under-reporting of the number of outbreaks, as well as the genotypes associated. | 2014 | 25223919 |
| a possible packaging signal in the rotavirus genome. | group a rotavirus (rva), an etiological agent of gastroenteritis in young mammals and birds, possesses a genome of 11 double-stranded rna segments. although it is believed that the rva virion contains one copy of each genomic segment and that the positive-strand rna (+rna) is incorporated into the core shell, the packaging mechanisms of rva are not well understood. here, packaging signals of rva were searched for by analyzing genomic sequences of mammalian and avian rva, which are considered to ... | 2014 | 25224974 |
| prevalence of endemic enteropathogens of calves in new zealand dairy farms. | to conduct a country-wide prevalence study of bovine group a rotavirus, coronavirus, cryptosporidium parvum, salmonella spp. and enterotoxigenic k99(+) escherichia coli (k99) in calves on new zealand dairy farms. | 2015 | 25237728 |
| prevalence and genomic characterization of g2p[4] group a rotavirus strains during monovalent vaccine introduction in brazil. | this study aims to: estimate the prevalence of g2p[4] rotaviruses in brazil between 2001-2011 from patients with acute gastroenteritis; perform phylogenetic analyses of g2p[4] brazilian strains (from vaccinated and non-vaccinated children) based on vp7 and vp8(∗) encoding genes and analyze the antigenic regions of these proteins comparing with rv1; and assess the full genetic background of eleven selected brazilian strains. the g2p[4] detection rate among rva positive samples was 0/157 in 2001, ... | 2014 | 25239525 |
| [evolutionary relationships of g3 garv isolated from pigs and humans in lulong county, hebei province, china]. | this study aimed to amplify major genome segments (vp7, vp4, vp6, vp2 and nsp2-5) of porcine g3 group a rotavirus (garv) llz212 isolated in our laboratory, determine their genotypes, and explore the evolutionary relationships between g3 garv strains isolated from humans and pigs in lulong county, hebei province, china. major genome segments of seven garv strains were amplified by reverse transcription-polymerase chain reaction (rt-pcr) and the segments were sequenced. the genome segments of seve ... | 2014 | 25272597 |
| classification and characterization of a laboratory chicken rotavirus strain carrying g7p[35] neutralization antigens on the genotype 4 backbone gene configuration. | the laboratory rotavirus strain, brs/115, has been used for more than two decades to monitor rotaviruses in specific pathogen free flocks of laying hens. however, the virus strain has not been characterized in detail. therefore we aimed at the description of molecular features of brs115 by using random primed reverse transcription-pcr of the genomic rna followed by massive parallel sequencing using the semiconductor sequencing technology. over 64,000 trimmed reads mapped to reference sequences o ... | 2014 | 25284347 |
| [evaluation of real-time multiplex pcr effectiveness for group a rotavirus genotyping]. | evaluate resolution and diagnostic significance of real-time multiplex pcr (mp rt-pcr) as a platform for group a rotavirus g/p genotyping test-systems. | 2014 | 25286527 |
| a bovine g8p[1] group a rotavirus isolated from an asymptomatically infected dog. | group a rotaviruses (rvas) are enteric pathogens with well-documented zoonotic transmissions to humans. the segmented genome of the virus enables reassortment events which might alter host susceptibility and/or disease course. genetic analysis of rotavirus in dogs has so far only revealed rvas with the vp7 and vp4 genome constellation g3p[3]. rva g3p[3] have also been found in cats, humans, monkeys and bats. in the present study, we described an unusual rva of genotype g8p[1] which was isolated ... | 2015 | 25304653 |
| detection of avian-like rotavirus a vp4 from a calf in japan. | a total of 568 normal feces from calves on a beef farm in fukui prefecture, japan, in 2011-2012 were examined by rt-semi-nested pcr for rotavirus a (rva) vp4 genes. through partial sequencing and blast analyses of 84 vp4-positive specimens, we identified an avian-like rva strain, n2342, which shares highest nucleotide identity (80.0%) with known avian-like bovine strain 993/83, in one specimen. phylogenetic analysis also revealed a close genetic relationship between n2342 and avian rvas, suggest ... | 2015 | 25311984 |
| analysis of rotavirus genotypes in korea during 2013: an increase in the g2p[4] genotype after the introduction of rotavirus vaccines. | group a rotavirus is the leading cause of acute gastroenteritis in children worldwide. we investigated g and p genotypes of group a rotavirus strains isolated from patients during 2013 and investigated which genotypes were identified from vaccinated patients. | 2014 | 25312273 |
| detection of rare g3p[19] group a rotavirus in human patient, italy. | infection with a rare g3p[19] rotavirus a strain was identified in an immunosuppressed patient in italy. the strain showed a p[19] viral protein 4 gene and a complete au-1-like genomic constellation. phylogenetic analyses showed high nucleotide identity between this strain and g3p[19] rotavirus a strains from asia, indicating possible reassortment events. | 2014 | 25340712 |
| isothermal cross-priming amplification implementation study. | cross-priming amplification (cpa) was evaluated for the early detection of norovirus (nv), rotavirus a (rv-a), enteric adenovirus (eadv) and astrovirus (astv). the analytical sensitivity of the cpa assay was 10(3) copies ml(-1) for nv, rv-a and astv detection and 10(4) copies ml(-1) for eadv detection. for each of the four pathogens, the positive detection rate by cpa was similar to real-time pcr methods and higher than the rate observed in an elisa. the detection coincidence rates of cpa and rt ... | 2015 | 25346035 |
| whole genotype constellation of prototype feline rotavirus strains frv-1 and frv64 and their phylogenetic relationships with feline-like human rotavirus strains. | feline rotaviruses, members of the species rotavirus a, are an infrequent source of zoonotic infections, and were previously shown by rna-rna hybridization assays to possess two distinct genomic rna constellations, represented by strains frv-1 and frv64. due to the lack of whole genome sequence information for frv-1, human rotavirus strain au-1 has been used as a surrogate for the genotype constellation of feline rotaviruses. the aim of this study was to determine the whole genome sequence of fr ... | 2015 | 25351516 |
| dna microarray for detection of gastrointestinal viruses. | gastroenteritis is a clinical illness of humans and other animals that is characterized by vomiting and diarrhea and caused by a variety of pathogens, including viruses. an increasing number of viral species have been associated with gastroenteritis or have been found in stool samples as new molecular tools have been developed. in this work, a dna microarray capable in theory of parallel detection of more than 100 viral species was developed and tested. initial validation was done with 10 differ ... | 2014 | 25355758 |
| acute diarrhea in adults consulting a general practitioner in france during winter: incidence, clinical characteristics, management and risk factors. | data describing the epidemiology and management of viral acute diarrhea (ad) in adults are scant. the objective of this study was to identify the incidence, clinical characteristics, management and risk factors of winter viral ad in adults. | 2014 | 25358721 |
| phylogenetic analysis of a g6p[5] bovine rotavirus strain isolated in a neonatal diarrhea outbreak in a beef cattle herd vaccinated with g6p[1] and g10p[11] genotypes. | the aim of this study was to perform the molecular characterization of the eleven genes of a g6p[5] bovine group a rotavirus (rva) strain detected in a diarrhea outbreak from a vaccinated beef cattle herd. the outbreak affected 80 % of calves between 15-30 days old. rva was identified by rt-pcr in 12 (70.6 %) out of 17 diarrheic fecal samples evaluated. the rotavirus wild-type strain had the genotype constellation g6(iv)-p[5](ix)-i2c-r2-c2-m2-a3-n2-t6-e2e-h3a. this study confirms the importance ... | 2015 | 25377636 |
| norovirus and rotavirus survival in urine collected from a public ecological sanitation system in ouagadougou, burkina faso. | urine from urine-diversion toilets (udts) is routinely used as fertilizer for urban agriculture in ouagadougou, burkina faso. because urine from udts can be accidentally spoiled by feces, we determined whether virulent enteric viruses could persist in urine that is used for agricultural purposes and pose a threat to human health. urine samples (n = 60) were first collected from 42 udts during the months of january and february 2012 in ouagadougou and screened negative for the presence of norovir ... | 2015 | 25408534 |
| molecular epidemiology of rotavirus in cats in the united kingdom. | rotaviruses are leading causes of gastroenteritis in the young of many species. molecular epidemiological studies in children suggest that interspecies transmission contributes to rotavirus strain diversity in people. however, population-based studies of rotaviruses in animals are few. we investigated the prevalence, risk factors for infection, and genetic diversity of rotavirus a in a cross-sectional survey of cats housed within 25 rescue catteries across the united kingdom. morning litter tray ... | 2015 | 25411173 |
| rotavirus infection in children and adults with acute gastroenteritis in thailand. | and young children, but rotavirus gastroenteritis in adults is uncommon. in this study, 260 stool samples collected in thailand from january 2006 to february 2007 from patients, of all ages with acute gastroenteritis, were tested for group a rotavirus and compared with rotavirus infections in children and adults. rota- virus was detected in 42% of the patients' samples, but children (< 18 years old) have a significantly higher prevalence (57%) of rotavirus infection than adults (≥ 18 years old) ... | 2014 | 25427349 |
| an outbreak of acute gastroenteritis associated with group a rotavirus in long-term care facility in slovenia. | in april 2013, an outbreak of acute gastroenteritis was reported in a residential long-term care facility (ltcf) in ljubljana. we carried out an outbreak investigation to describe the outbreak, identify factors associated with contracting infection and to recommend control measures. | 2015 | 25447968 |
| whole genomic analysis of porcine g10p[5] rotavirus strain p343 provides evidence for bovine-to-porcine interspecies transmission. | porcine group a rotavirus (rva) strain p343 (rva/pig-tc/tha/p343/1991/g10p[5]) was suggested to have vp7 and vp4 genes of bovine origin. in order to obtain precise information on the exact origin and evolution of this unusual porcine strain, the remaining nine genes (vp6, vp1-3, and nsp1-5) of strain p343 were sequenced and analyzed in the present study. on whole genomic analysis, strain p343 was found to have a bovine rva-like genotype constellation (g10-p[5]-i2-r2-c2-m2-a3-n2-t6-e2-h3) differe ... | 2014 | 25457370 |
| full genome characterization of human rotavirus a strains isolated in cameroon, 2010-2011: diverse combinations of the g and p genes and lack of reassortment of the backbone genes. | over the past few years whole genome sequencing of rotaviruses has become a routine laboratory method in many strain surveillance studies. to study the molecular evolutionary pattern of representative cameroonian rotavirus a (rva) strains, the semiconductor sequencing approach was used following random amplification of genomic rna. in total, 31 rva strains collected during 2010-2011 in three cameroonian study sites located 120 to 1240 km from each other were sequenced and analyzed. sequence anal ... | 2014 | 25460824 |
| relationship between viral detection and turbidity in a watershed contaminated with group a rotavirus. | enteric viruses are present in aquatic environments due to contamination by raw sewage, even in the absence of fecal coliforms, which are considered to be significant indicators when it comes to microbial water quality assessment. this study investigated the presence of group a rotavirus (rva) in surface water from a river basin in minas gerais, brazil, assessing the influence of the urbanization, the rainfall, and the microbiological and physico-chemical parameters regarding water quality. fort ... | 2015 | 25471713 |
| full genome sequence of a reassortant human g9p[4] rotavirus strain. | this is a report of the complete genomic sequence of a reassortant rotavirus group a g9-p[4]-i2-r2-c2-m2-a2-n2-t2-e6-h2 strain designated rva/human-wt/usa/ lb1562/2010/g9p[4]. | 2014 | 25502675 |
| rotavirus infection in children and adults with acute gastroenteritis in thailand. | and young children, but rotavirus gastroenteritis in adults is uncommon. in this study, 260 stool samples collected in thailand from january 2006 to february 2007 from patients, of all ages with acute gastroenteritis, were tested for group a rotavirus and compared with rotavirus infections in children and adults. rota- virus was detected in 42% of the patients' samples, but children (< 18 years old) have a significantly higher prevalence (57%) of rotavirus infection than adults (≥ 18 years old) ... | 2014 | 25507599 |
| detection and genetic characterization of porcine group a rotaviruses in asymptomatic pigs in smallholder farms in east africa: predominance of p[8] genotype resembling human strains. | viral enteritis is a serious problem accounting for deaths in neonatal animals and humans worldwide. the absence of surveillance programs and diagnostic laboratory facilities have resulted in a lack of data on rotavirus associated diarrheas in pigs in east africa. here we describe the incidence of group a rotavirus (rva) infections in asymptomatic young pigs in east africa. of the 446 samples examined, 26.2% (117/446) were positive for rva. more nursing piglets (78.7%) shed rva than weaned (32.9 ... | 2015 | 25541378 |
| rapid and simultaneous detection of three major diarrhea-causing viruses by multiplex real-time nucleic acid sequence-based amplification. | rotaviruses, noroviruses and astroviruses are the major viral pathogens leading to diarrhea worldwide. epidemiological investigations of outbreaks associated with these viruses have been impeded by the lack of methods for quick diagnosis and detection. in the current study, a multiplex real-time nucleic acid sequence-based amplification (rt-nasba) system was developed for the simultaneous detection of rotavirus a/norovirus genogroup ii/astrovirus. the specificity and sensitivity of the assay wer ... | 2015 | 25559674 |
| diversity of rotavirus strain circulated in gwangju, republic of korea. | the introduction of new rotavirus vaccines into the public sphere makes it necessary to maintain constant surveillance and to heighten public awareness of the appearance of new rotavirus strains. we describe the molecular epidemiology of circulating rotavirus strains after vaccine introduction. | 2014 | 25562046 |
| multicenter evaluation of the biofire filmarray gastrointestinal panel for etiologic diagnosis of infectious gastroenteritis. | the appropriate treatment and control of infectious gastroenteritis depend on the ability to rapidly detect the wide range of etiologic agents associated with the disease. clinical laboratories currently utilize an array of different methodologies to test for bacterial, parasitic, and viral causes of gastroenteritis, a strategy that suffers from poor sensitivity, potentially long turnaround times, and complicated ordering practices and workflows. additionally, there are limited or no testing met ... | 2015 | 25588652 |
| aetiology of acute paediatric gastroenteritis in bulgaria during summer months: prevalence of viral infections. | paediatric acute gastroenteritis is a global public health problem. comprehensive laboratory investigation for viral, bacterial and parasitic agents is helpful for improving management of acute gastroenteritis in health care settings and for monitoring and controlling the spread of these infections. our study aimed to investigate the role of various pathogens in infantile diarrhoea in bulgaria outside the classical winter epidemics of rotavirus and norovirus. stool samples from 115 hospitalized ... | 2015 | 25596126 |
| genetic diversity in three bovine-like human g8p[14] and g10p[14] rotaviruses suggests independent interspecies transmission events. | the group a rotavirus (rva) p[14] genotype has been detected sporadically in humans and is thought to be acquired through zoonotic transmission. the present study describes the full-length genome analysis of two g8p[14] and one g10p[14] human rvas detected in italy. the strains possessed the typical bovine-like i2-r2-c2-m2-a3/a11-n2-t6-e2-h3 genotype constellation. all the segments of the two g8p[14] rvas were most closely related to bovine(-like) strains but were relatively distant to each othe ... | 2015 | 25614586 |
| rotavirus and adenovirus gastroenteritis: time series analysis. | this study investigated the effects of changes in weather conditions (monthly average temperature, monthly minimum temperature, monthly average humidity) on rotavirus and adenovirus gastroenteritis frequency and whether there was a seasonal correlation. | 2015 | 25625610 |
| full-genome sequencing of a hungarian canine g3p[3] rotavirus a strain reveals high genetic relatedness with a historic italian human strain. | a canine rotavirus a strain was identified in the fecal specimen of a young dog during 2012 in hungary. the strain rva/dog-wt/hun/135/2012/g3p[3] shared complete genotype constellation (g3-p[3]-i3-r3-c3-m3-a15-n2-t3-e3-h6) and high genome sequence similarity (nt, 98.8 %) with a historic human strain, rva/human-tc/ita/pa260-97/1997/g3p[3]. this study provides evidence for the canine origin of the unusual nsp1 genotype, a15, and reinforces the hypothesis of direct interspecies transmission of cani ... | 2015 | 25634124 |
| molecular characterization of equine rotaviruses isolated in europe in 2013: implications for vaccination. | equine group a rotavirus (rvas) mainly cause disease in foals under the age of 3 months. only sporadic data are available on the circulation of rvas in equine populations in europe. in this study, 65 diarrheic samples from foals under 4 months of age were collected in belgium (n=32), germany (n=17), slovenia (n=5), sweden (n=4), hungary (n=3), italy (n=2), france (n=1) and the netherlands (n=1). forty percent of these samples (n=26) were found to be rva positive by a quantitative rt-pcr assay. t ... | 2015 | 25637313 |
| molecular epidemiology of group a rotavirus among children admitted to hospital in salto, uruguay, 2011-2012: first detection of the emerging genotype g12. | group a rotavirus (rva) is the most important etiologic agent of infant acute gastroenteritis (age) worldwide. detection and molecular characterization of rva in salto department, northwestern region of uruguay, was conducted on 175 clinical samples, being 153 stool and 22 vomit samples, collected from hospitalized children with age, between 0-15 years old, from two hospitals of salto city during 2011 and 2012. rva was detected and genotyped by seminested multiplex rt-pcr in order to determine g ... | 2015 | 25650154 |
| prevalence of rotaviruses groups a and c in egyptian children and aquatic environment. | the objective of this study is to compare the prevalence of rotaviruses groups a and c in egyptian children and aquatic environment. from 110 stool specimens of children with acute diarrhea and using rt-pcr, 35 samples (31.8 %) were positive for human rotavirus group a and 15 samples (13.6 %) were positive for human rotavirus group c. from 96 samples collected from zenin wastewater treatment plant over a 2-year period (november 2009-october 2011) and using rt-pcr, rotavirus group a was detected ... | 2015 | 25665825 |
| epidemiological and clinical studies of rotavirus-induced diarrhea in china from 1994-2013. | group a rotavirus infections cause diarrhea in infants and primarily occur in winter. these viruses are characterized by the viral structural proteins vp7 (g subtypes) and vp4 (p subtypes). current treatments employ vaccines combined with symptomatic treatments. through a review of published papers from 2003-2013, which included articles, theses, dissertations and academic conference proceedings in pubmed and cnki (china national knowledge infrastructure), we examined the epidemiological and cli ... | 2014 | 25668671 |
| rotavirus associated gastroenteritis in thailand. | group a rotavirus is the leading cause of severe diarrhea in infants and young children, and in young animals of many species worldwide. rotavirus is also the major cause of deaths of children younger than 5 years of age, particularly, in developing countries in asia and africa. in thailand, the burden of rotavirus infection rate in children admitted to the hospitals with acute gastroenteritis ranged from 28.4 to 44.5 %. the seasonality of rotavirus gastroenteritis in thailand was detected all-y ... | 2014 | 25674586 |
| rotavirus strains in neglected animal species including lambs, goats and camelids. | surveillance of rotavirus infections and circulating strains in small ruminants (i.e. lambs, goats and camelids) has been a neglected research area in the past. however, recent years that have seen an intensification of surveillance in humans and livestock animals, where vaccines to reduce disease burden caused by rotavirus a (rva) are available, led to the efforts to better understand the epidemiology, ecology and evolution of rva strains in other hosts, including lambs, goats and camelids. the ... | 2014 | 25674588 |
| changing patterns of rotavirus strains circulating in ireland: re-emergence of g2p[4] and identification of novel genotypes in ireland. | worldwide, group a rotavirus (rva) is recognized as the most common aetiological agent of acute diarrheal disease in children. one hundred and ninety seven positive faecal samples were obtained from patients between 2006 and 2008. reverse transcriptase polymerase chain reaction (rt-pcr) was used to amplify the vp7 and vp4 gene segments of these samples, and g and p typing was carried out subsequently. the most common strain type was g1p[8], and the emergent global g9-type was identified in both ... | 2015 | 25704492 |
| whole-genome sequence analysis of g3 and g14 equine group a rotaviruses isolated in the late 1990s and 2009-2010. | equine group a rotavirus (rva) g3p[12] and g14p[12] strains cause gastroenteritis in foals worldwide. both of these strains have been co-circulating in japan since g14p[12] strains emerged in the late 1990s. although it is important to comprehensively understand the evolution of rva strains, whole-genome sequence data on recent equine rva strains in japan are lacking. therefore, in this study, whole-genome analysis of 23 equine rva isolates from the late 1990s and 2009-2010 and the vaccine strai ... | 2015 | 25708838 |
| microbial aetiology of acute diarrhoea in children under five years of age in khartoum, sudan. | diarrhoea is one of leading causes of morbidity and mortality worldwide. recent estimations suggested the number of deaths is close to 2.5 million. this study examined the causative agents of diarrhoea in children under 5 years of age in suburban areas of khartoum, sudan. a total of 437 stool samples obtained from children with diarrhoea were examined by culture and pcr for bacteria, by microscopy and pcr for parasites and by immunoassay for detection of rotavirus a. of the 437 samples analysed, ... | 2015 | 25713206 |
| h2 genotypes of g4p[6], g5p[7], and g9[23] porcine rotaviruses show super-short rna electropherotypes. | during group a rotavirus (rva) surveillance of pig farms in japan, we detected three rva strains (g4p[6], g5p[7], and g9p[23] genotypes), which showed super-short rna patterns by polyacrylamide gel electrophoresis, in samples from a healthy eight-day-old pig and two pigs of seven and eight days old with diarrhea from three farms. reverse transcription pcr and sequencing revealed that the full-length nsp5 gene of these strains contained 952 or 945 nucleotides, which is consistent with their super ... | 2015 | 25724331 |
| human rotavirus group a serotypes causing gastroenteritis in children less than 5 years and hiv-infected adults in viwandani slum, nairobi. | rotavirus remains a leading cause of acute gastroenteritis in children worldwide with an estimated 2000 deaths each day in developing countries. due to hiv/aids scourge in kenya, it is possible that rotavirus-related gastroenteritis has been aggravated in adults. the global alliance for immunizations has ranked rotavirus infection a priority for vaccine, and, to ensure its success, there is a need to document the local strain(s) circulating in different regions. | 2015 | 25733783 |
| unexpected spreading of g12p[8] rotavirus strains among young children in a small area of central italy. | rotavirus gastroenteritis is associated mainly with the five genotypes g1,3,4,9p[8] and g2p[4] that are common worldwide, but emerging strains including g6, g8, and g12 are also reported sporadically. g12p[8] rotavirus was observed unexpectedly to spread in a limited area of italy during the rotavirus surveillance season 2012-2013. all strains were genotyped for vp7 and vp4 and subjected to phylogenetic analysis. amino acid sequences of antigenic regions were compared with vaccine and field stra ... | 2015 | 25758365 |
| crystal structure of the c-terminal 2',5'-phosphodiesterase domain of group a rotavirus protein vp3. | in response to viral infections, the mammalian innate immune system induces the production of the second messenger 2'-5' oligoadenylate (2-5a) to activate latent ribonuclease l (rnase l) that restricts viral replication and promotes apoptosis. a subset of rotaviruses and coronaviruses encode 2',5'-phosphodiesterase enzymes that hydrolyze 2-5a, thereby inhibiting rnase l activation. we report the crystal structure of the 2',5'-phosphodiesterase domain of group a rotavirus protein vp3 at 1.39 å re ... | 2015 | 25758703 |
| complete genomic sequence for an avian group g rotavirus from south africa. | we report the first complete sequence for an avian group g rotavirus (rvg) genome from africa, which is the third publically available rvg genome. these rvg genomes are highly diverse, especially in their vp4, vp7, nsp4, and nsp3 segments, indicating that rvg diversity is comparable to that of rotavirus a. | 2015 | 25767240 |
| molecular characterization of group a rotavirus strains detected in children with diarrhea admitted to nigerian hospitals in 2013. | group a rotaviruses (rva) are the leading cause of acute gastroenteritis (age) in children worldwide and cause up to 455,000 deaths annually, mostly in developing countries. during 2013, 66 rvas from children with age admitted to four nigerian hospitals were investigated. the g3p[6], g1p[8] and g2p[4] genotypes predominated. the vp7 and/or vp4 genes of 18 g3p[6]/[8]/[4], six g2p[4], three g12p[8]/[4], and two g1p[8] rva strains were sequenced. the g3p[6] strains belonged to lineage g3-iii and we ... | 2015 | 25772575 |
| genomic characterization and molecular investigation of vp7 epitopes of uncommon g10p[8] group a rotavirus strains detected in italy in 2009. | rotavirus strains with the uncommon genotype g10 have been detected sporadically in cases of acute gastroenteritis in humans and are thought to be transmitted zoonotically. during 2009, 10 g10p[8] rotavirus strains were detected in the stools of children hospitalized with acute diarrhoea in several paediatric hospitals in italy. the phylogenetic analysis of the vp7 gene of the italian g10p[8] strains analysed revealed nucleotide identities ranging from 94 to 99 %. molecular characterization of t ... | 2015 | 25783473 |