Publications

TitleAbstractYear
Filter
PMID(sorted ascending)
Filter
spermidine levels are implicated in heavy metal tolerance in a spermidine synthase overexpressing transgenic european pear by exerting antioxidant activities.to verify whether spermidine synthase (spds) can confer long-term multi-heavy metal tolerance, in vitro shoots of a transgenic european pear (pyrus communis l. 'ballad') line #32 overexpressing apple spds (mdspds1), as well as a wild type (wt) line, were subjected to stress using either cdcl(2), pbcl(2), zncl(2), or a combination thereof. based on either shoot height increment or fresh weight and morphological changes upon heavy metal stress, the performance of the transgenic line #32 was better ...201019544002
mycobased synthesis of silver nanoparticles and their incorporation into sodium alginate films for vegetable and fruit preservation.biosynthesis of silver nanoparticles using trichoderma viride and their incorporation into sodium alginate for vegetable and fruit preservation has been demonstrated in this study. aqueous silver (ag(+)) ions when exposed to the filtrate of t. viride are reduced in solution. these extremely stable silver nanoparticles were characterized by means of uv-vis spectrophotometer, ftir, tem, and eds. the nanoparticles exhibit maximum absorbance at 421 nm in the uv spectrum. the presence of proteins was ...200919552418
effect of aqueous extracts of alligator pear seed (persea americana mill) on blood glucose and histopathology of pancreas in alloxan-induced diabetic rats.effects of aqueous extract of alligator pear seed on normal and alloxan-induced diabetic rats were investigated in 6 groups of rats (5 rats per group). test groups were made diabetic with intra-peritoneal injection of alloxan and treated with 300 mg and 600 mg/kg body weight of alligator pear seed extract. two non-diabetic groups were also administered with 300 mg and 600 mg/kg body weight extract. the levels of blood glucose were examined in all 6 experimental groups. in diabetic rats, blood gl ...200919553173
fatty acid composition in lipid fractions lengthwise the mycelium of mortierella isabellina and lipid production by solid state fermentation.this paper investigates the correlation between mycelial age and fatty acid biosynthesis. the correlation was investigated by analyzing the lipid composition lengthwise the mycelium of the oleaginous fungus mortierella isabellina, a potential producer of gamma-linolenic acid (gla). young mycelia were rich in polar lipids (glycolipids plus sphingolipids and phospholipids), while neutral lipid content increased in aged mycelia. in young mycelia, each polar lipid fraction contained almost 40% (w/w) ...200919574039
development of an improved isolation approach and simple sequence repeat markers to characterize phytophthora capsici populations in irrigation ponds in southern georgia.phytophthora capsici, the causal agent of phytophthora blight, is a major concern in vegetable production in georgia and many other states in the united states. contamination of irrigation water sources by p. capsici may be an important source of inoculum for the pathogen. a simple method was developed in this study to improve the efficiency of recovering p. capsici from fruits used as baits in irrigation ponds. in contrast to direct isolation on agar plates, infected fruit tissues were used to ...200919581483
seasonal occurrence and impact of halyomorpha halys (hemiptera: pentatomidae) in tree fruit.halyomorpha halys is an introduced stink bug species from asia that is spreading throughout the mid-atlantic united states. it is native to south korea, japan, and eastern china, where it is an occasional pest of tree fruit, including apple and pear. cage experiments with adults placed on apple and peach during critical plant growth stages demonstrate that it can cause damage to developing fruit during mid- and late season growth periods and that feeding occurs on all regions of the fruit. feedi ...200919610429
light, electron microscopy and histopathology of myxobolus salminus n. sp., a parasite of salminus brasiliensis from the brazilian pantanal.in this report, we describe the morphology and histopathology of myxobolus salminus n. sp., a parasite of the gill filaments of wild salminus brasiliensis (dourado) from the brazilian pantanal. the small polysporic plasmodia were approximately 100 microm in diameter and the development was asynchronous. the mature spores were oval to pear shaped and had a smooth wall. the spore measurements were (mean+/-s.d., with range in parentheses): length 10.1+/-0.4 microm (9.6-10.5), width 6.1+/-0.4 microm ...200919640650
influence of post-harvest treatments with fludioxonil and soy lecithin co-application in controlling blue and grey mould and fludioxonil residues in coscia pears.the residue levels of fludioxonil (flu) were determined in coscia pear following a 1-, 2- or 4-min dip in an aqueous mixture of flu containing 300 or 100 mg l(-1) (active ingredient, a.i.) at 20 and 50 degrees c, respectively, with or without 2% soy lecithin. the efficacy of heat treatment with water and flu mixtures was investigated on artificially inoculated pears for the control of post-harvest decay caused by blue (penicillium expansum link) and grey (botrytis cinerea pers. ex fr.) mould. tr ...200919680873
complex combined vascular malformations and vascular malformation syndromes affecting the extremities in children.complex combined vascular malformations affecting extremities are an interesting group of vascular malformations because, in addition to the vascular channel anomalies present, they can be associated with other tissue changes and sometimes altered limb growth. at present, magnetic resonance imaging is the gold standard imaging tool to evaluate such complex conditions in children because of its inherent tissue specificity and vascular capabilities that enables characterization of tissues and the ...200919724993
description of cryptosporiopsis kienholzii and species profiles of neofabraea in major pome fruit growing districts in the pacific northwest usa.the objectives of this study were i) to give a taxonomic description of a fungus phylogenetically related to neofabraea and assign the name cryptosporiopsis kienholzii to this fungus, ii) to expand previous neofabraea species profiles from infected apple and pear fruit collected from major pome fruit production districts in oregon and washington, and iii) to determine the sensitivity of neofabraea alba, neofabraea malicorticis, neofabraea perennans, and c. kienholzii to a range of fungicides. a ...200919733662
endodontic management of horizontally placed molars after gunshot injury to mandible: a case report.a 37-year-old man reported to our department with the history of gunshot injury to the mandible 15 years before. his anterior mandible had been resected earlier and bone graft was seen. intraoral examination of lower jaw revealed 4 remaining mandibular molars. these teeth were severely rotated such that they lay horizontal with respect to the mandibular base. preoperative dentascan spiral computerized tomography (ct) of the patient revealed obliteration of the mesial canals of the mandibular rig ...200919734072
functional analysis of the n terminus of the erwinia amylovora secreted effector dspa/e reveals features required for secretion, translocation, and binding to the chaperone dspb/f.dspa/e is a type iii secreted effector protein required for pathogenicity in the apple and pear pathogen erwinia amylovora, and dspb/f is a small chaperone protein involved in dspa/e secretion. while the secretion and translocation signals of many type iii secretion effector proteins in human enteric pathogens have been characterized extensively, relatively little is known about the translocation requirements of many effectors in plant pathogens, including large dspe-like proteins. in this study ...200919737101
update on comparative genome mapping between malus and pyrus.comparative genome mapping determines the linkage between homologous genes of related taxa. it has already been used in plants to characterize agronomically important genes in lesser studied species, using information from better studied species. in the maloideae sub-family, which includes fruit species such as apple, pear, loquat and quince, genome co-linearity has been suggested between the genera malus and pyrus; however map comparisons are incomplete to date.200919747407
genotypic comparison of pantoea agglomerans plant and clinical strains.pantoea agglomerans strains are among the most promising biocontrol agents for a variety of bacterial and fungal plant diseases, particularly fire blight of apple and pear. however, commercial registration of p. agglomerans biocontrol products is hampered because this species is currently listed as a biosafety level 2 (bl2) organism due to clinical reports as an opportunistic human pathogen. this study compares plant-origin and clinical strains in a search for discriminating genotypic/phenotypic ...200919772624
diuretic and antioxidant effects of cacti-nea, a dehydrated water extract from prickly pear fruit, in rats.dehydrated extract of the prickly pear fruit opuntia ficus indica, cacti-nea, was evaluated for its chronic diuretic and antioxidant effects in wistar rats. cacti-nea was orally administered daily for seven days at the dose of 240 mg/kg/day. a positive group was orally treated with hydrochlorothiazide at the dose of 10 mg/kg/day and a control group with vehicle. daily measurements of body weight, urine volume, and concentration of sodium, potassium and uric acid in urine were performed for each ...201019777503
radiological and ct findings in a extensive upper-limb involvement of gorham's disease: a case report.the gorham-stout syndrome (gorham's massive osteolysis) is a rare condition in which spontaneous, progressive resorption of bone occurs. the etiology is poorly understood.200919829979
determination and confirmation of nicotinic acid and its analogues and derivates in pear and apple blossoms using high-performance liquid chromatography-diode array-electrospray ionization mass spectrometry.erwinia amylovora causes fire blight, a serious disease of apple and pear. the bacterial pathogen colonizes the flower stigma and hypanthium, where it multiplies and then invades through natural openings (nectarthodes). e. amylovora requires nicotinic acid as growth factor, and competition for nicotinic acid is being explored as a novel biocontrol strategy. the ability of e. amylovora to substitute nicotinic acid with analogues or derivates as growth factors has not been investigated yet. furthe ...200919835358
the nature of the growth rate.1. the growth rate of organisms may be considered as a chemical reaction which gives the mature organism as its end-product. the organism grows at a definite rate which is, at any moment, proportional to the amount of growth yet to be made. 2. shoots of young pear trees measured at weekly intervals during the growing season showed a rate similar to that of an autocatalytic reaction. 3. young walnut trees showed distinct cycles of growth in a single season, but the growth in each cycle proceeded ...192019871830
[cystoscopy in a heifer with rupture of a patent urachus].this case report describes the clinical, ultrasonographic and cystoscopic findings and treatment in a two-year-old swiss braunvieh heifer with rupture of a patent urachus. the lead signs in the seven-month-pregnant heifer were markedly abnormal general condition and demeanour and a pear-shaped abdomen. the heifer had severe azotaemia, and abdominal ultrasonography revealed ascites, which was diagnosed as uroperitoneum based on an elevated creatinine level in the fluid. a patent urachus was ident ...200919885799
quantitative detection of pear-pathogenic stemphylium vesicarium in orchards.abstract isolates of stemphylium vesicarium causing brown spot of pear can be distinguished from nonpathogenic isolates of s. vesicarium from pear or from other hosts on the basis of distinctive amplified fragment length polymorphism fingerprinting profiles. dna fragments specific for isolates pathogenic to pear were identified and a quantitative polymerase chain reaction (pcr) was developed on the sequence from one of these specific dna loci. this taqman pcr has a high sensitivity with a dynami ...200919900004
clostridium arbusti sp. nov., an anaerobic bacterium isolated from pear orchard soil.an obligately anaerobic, gram-positive, spore-forming bacterial strain, designated sl206(t), was isolated from pear orchard soils. strain sl206(t) cells were straight or slightly curved rods, with motility by peritrichate flagella. cell walls contained meso-diaminopimelic acid; wall sugars were glucose, rhamnose and mannose. the major fatty acids were c(16 : 0), c(18 : 1)omega9c and summed feature 10 (containing c(18 : 1)omega11c/9t/6t). api 20a reactions were negative for oxidase, catalase and ...201019915114
determination of the insecticide imidacloprid in fruit juices using micellar high-performance liquid chromatography.a simple and reliable hplc method was developed to determine imidacloprid, a chloronicotinyl insecticide that has a highly specific affinity to the nicotinic acetylcholine receptor of insects, above its permissible limit of consumption in fruit juices (orange, apple, and a mixture of pineapple and pear). samples were injected directly into a kromasil c18 column, without any pretreatment step, using the micellar mobile phase 0.10 m sodium dodecyl sulfate and 2.5% (v/v) propanol buffered at ph 7 a ...201219916392
plasma vegf determination in disseminated lymphangiomatosis-gorham-stout syndrome: a marker of activity? a case report with a 5-year follow-up.disseminated lymphangiomatosis and gorham-stout disease are being considered as two forms of a single rare disease, characterized by a proliferation of lymphatic vessels, triggered by lymphangiogenic factors. there is no biological marker of the disease. plasma vegf might be a useful tool since the recent demonstration of its pivotal role in the mechanism of this disease. a 45-year-old woman with a history of disseminated lymphangiomatosis involving mediastinum, retroperitoneum, spleen and syste ...201019931435
purification and biochemical characterization of polygalacturonase produced by penicillium expansum during postharvest decay of 'anjou' pear.a polygalacturonase (pg) was extracted and purified from decayed tissue of 'anjou' pear fruit inoculated with penicillium expansum. ammonium sulfate precipitation, gel filtration, and cation exchange chromatography were used to purify the enzyme. both chromatographic methods revealed a single peak corresponding to pg activity. pg enzyme activity from healthy and wounded pear tissue was undetectable, which supports the claim that the purified pg is of fungal origin. the purified enzyme had a mole ...201019968548
gorham-stout syndrome: a rare clinical entity and review of literature.gorham-stout syndrome is a very rare bone condition of unknown etiology. it is characterized by the spontaneous onset of bone resorption. bones that previously appeared normal begin to resorb, partially or completely. sometimes only a thin shell of cortical bone remains, and there is usually a little replacement by fibrous tissue. this process can continue for years but may stop spontaneously. bone loss can occur in one bone or spread to soft tissue and adjacent bones. although the disease may s ...201019969488
the histology and histopathology of the dental innervation: (section of odontology).the presence of a perivascular neural plexus in the periodontal membrane suggests that the dental structures have both sensory and autonomic nerve supply. the fibres described by mummery are unaffected by section of the inferior dental nerve although there is marked degeneration in all demonstrable nerves. nerve-fibres have been observed describing simple and complex looping in the odontogenetic zone and others which, running tangentially between the odontoblast layer and the dentine, form a ver ...193919992023
compatible and self-incompatible pollination in pyrus communis displays different polyamine levels and transglutaminase activity.the polyamine (pa) content and the transglutaminase (tgase) activity have been investigated in pyrus communis pollination with compatible and self-incompatible (si) pollen in order to deepen their possible involvement in the progamic phase of plant reproduction. the pa distribution as free, perchloric acid (pca)-soluble and pca-insoluble fractions in ungerminated (ugp), germinating pollen (gp), styles and pollinated styles with compatible and si pollens is discussed in the light of a possible ro ...201020020163
conservative treatment of a pathologic fracture in a patient with gorham-stout disease.gorham-stout disease, or massive osteolysis, is an extremely rare idiopathic condition characterized by spontaneous, localized relentless resorption of one or several contiguous bones. there is no known successful treatment. autologous bone graft also resorbs. it is a condition with difficult diagnosis, treatment, and prognosis. we report an extreme case of massive osteolysis of the maxillofacial complex. unique to this case is that the patient presented with a mandibular fracture, and was takin ...201020034818
proteomic analysis of alternaria alternata (fr.) keissler responds to cos fumigation.carbonyl sulfide (cos) is a new fumigant which has been a potential alternative to methyl bromide and phosphine in many applications. in this study, we investigated the fungitoxicity of cos towards the pathogen of pear black spot disease alternaria alternata (fr.) keissler (a. alternata). moreover, proteomic analysis and rt-pcr was performed and our results showed that during the fumigation, the regulation of 21 proteins in protein expression and mrna accumulation levels is involved, which respo ...201020036892
paramphistomum cervi: surface topography of the tegument of adult fluke.adult paramphistomum cervi or rumen fluke are pear-shaped, slightly concave ventrally and convex dorsally. the worm measures about 5-13 mm in length and 2-5 mm in width across the mid-section. as observed by scanning electron microscopy (sem), the tegumental surface in all part of the body, appears highly corrugated with transverse folds alternating with grooves and is spineless. at high magnification, the surface of the fold is composed of microfolds or ridges separated by microgrooves or pits. ...201020045698
identification of genes differentially expressed during interaction of resistant and susceptible apple cultivars (malus x domestica) with erwinia amylovora.the necrogenic enterobacterium, erwinia amylovora is the causal agent of the fire blight (fb) disease in many rosaceae species, including apple and pear. during the infection process, the bacteria induce an oxidative stress response with kinetics similar to those induced in an incompatible bacteria-plant interaction. no resistance mechanism to e. amylovora in host plants has yet been characterized, recent work has identified some molecular events which occur in resistant and/or susceptible host ...201020047654
complete genome sequence of the fire blight pathogen erwinia pyrifoliae dsm 12163t and comparative genomic insights into plant pathogenicity.erwinia pyrifoliae is a newly described necrotrophic pathogen, which causes fire blight on asian (nashi) pear and is geographically restricted to eastern asia. relatively little is known about its genetics compared to the closely related main fire blight pathogen e. amylovora.201020047678
temperature-dependent emergence of osmia cornifrons (hymenoptera: megachilidae) adults.japanese hornfaced bees osmia cornifrons (radoszkowski) (hymenoptera: megachilidae) are used for pollination of spring blooming fruit crops such as apple, pear, and blueberry. because o. cornifrons has a short adult life span, synchronization of bee emergence with bloom is critical to maximize crop pollination. this study was conducted to determine lower temperature thresholds (ltds), optimum temperatures, and required degree-day accumulation for emergence of o. cornifrons adults. patterns of te ...200920069827
use of zoledronic acid in the treatment of gorham's disease.gorham's disease (gorham-stout syndrome) is a rare condition of unknown etiology involving a localized endothelial proliferation of lymph vessels resulting in destruction with bone resorption. the syndrome is rarely seen in the facial skeleton and has a large variety of prognoses and treatments. a case of this syndrome in a 9-year-old boy is presented. the clinical aspects are described, together with the treatment involving zoledronic acid. other treatments described in the literature are revie ...201020074817
[gorham syndrome, a rare disease...]. 201020087270
[sensitization to pollens of oleaceae family in a group of patients from mexico city].in mexico city, where the olive trees are scarce, the oleaceae family has been recognized as one of the most important causes of pollen allergy, possibly related to cross-reactivity with other pollens of the oleaceae family, as fraxinus excelsior, ligustrum vulgaris and osmanthus americanus.201520088452
complete genome sequence of the plant pathogen erwinia amylovora strain atcc 49946.erwinia amylovora causes the economically important disease fire blight that affects rosaceous plants, especially pear and apple. here we report the complete genome sequence and annotation of strain atcc 49946. the analysis of the sequence and its comparison with sequenced genomes of closely related enterobacteria revealed signs of pathoadaptation to rosaceous hosts.201020118253
commentary: massive osteolysis or gorham-stout disease. 201020123408
food allergy due to olive.we report the case of a 28-year-old man who presented palatal itching and genaralized urticaria following ingestion of olive 3 years after being diagnosed with olive pollinosis. the patient did not have a history of food allergy or urticaria. the results of skin prick tests with aeroallergens including latex were positive for house dust mite and olive pollen. the results of prick tests and prick-to-prick tests for olive fruit were positive, as were those of specific immunoglobulin e tests to oli ...200920128426
gorham-stout disease of the temporal bone.gorham-stout (gs) disease is a rare disease of the bone and is also known as massive osteolysis. less than 200 cases have been reported in the world literature. a 29-year-old female with a diagnosis of gs disease was identified. she complained of aural fullness and tinnitus bilaterally. demineralization and moth-eaten changes of the osseous structures of the skull base and posterior fossa were prominent. the left mastoid air cells were opacified and erosion extended to the left jugular foramen, ...201020131367
transcriptional studies of the hrpm/opgh gene in pseudomonas syringae during biofilm formation and in response to different environmental challenges.pseudomonas syringae pv. syringae strain ff5 is a phytopathogen that causes a rapid dieback on ornamental pear trees. in the present study, the transcriptional expression of hrpm/opgh, algd, hrpr and rpod was evaluated in p. syringae ff5 and ff5.m2 (hrpm/opgh mutant). the temporal expression of these genes was evaluated during biofilm formation, the hypersensitive reaction (hr) on tobacco plants, and when the bacteria were subjected to different environmental stresses. the results indicate that ...201020132277
structure of sterol aliphatic chains affects yeast cell shape and cell fusion during mating.under mating conditions, yeast cells adopt a characteristic pear-shaped morphology, called a "shmoo," as they project a cell extension toward their mating partners. mating partners make contact at their shmoo tips, dissolve the intervening cell wall, and fuse their plasma membranes. we identified mutations in erg4, encoding the enzyme that catalyzes the last step of ergosterol biosynthesis, that impair both shmoo formation and cell fusion. upon pheromone treatment, erg4delta mutants polarized gr ...201020150508
massive cranial osteolysis, skin changes, growth retardation and developmental delay: gorham syndrome with systemic manifestations?we report on a 16-month-old girl with multiple swellings on her skull due to massive osteolysis, growth retardation, facial anomalies, and wrinkly skin with mosaic hypopigmentation. she also had severe hypercalcemia, which gradually returned to normal levels. the condition likely represents gorham syndrome with systemic manifestations.201020186788
prebiotic effect of fruit and vegetable shots containing jerusalem artichoke inulin: a human intervention study.the present study aimed to determine the prebiotic effect of fruit and vegetable shots containing inulin derived from jerusalem artichoke (ja). a three-arm parallel, placebo-controlled, double-blind study was carried out with sixty-six healthy human volunteers (thirty-three men and thirty-three women, age range: 18-50 years). subjects were randomised into three groups (n 22) assigned to consume either the test shots, pear-carrot-sea buckthorn (pcs) or plum-pear-beetroot (ppb), containing ja inul ...201020187995
application of a potentiometric sensor array as a technique in sensory analysis.this paper reports on the application of a potentiometric sensor array used for monitoring changes in probiotic fermented milk during storage, classification of probiotic fermented milk according to flavor and to accurately predict the results from a human sensory panel. for that purpose the potentiometric electronic tongue consisting of seven sensors and an ag/agcl reference electrode was used. the samples of plain, strawberry, apple-pear and forest-fruit probiotic fermented milk were stored du ...201020188937
oxidative stress and senescence-like status of pear calli co-cultured on suspensions of incompatible quince microcalli.this work presents a simple in vitro system to study physiological, biochemical and molecular changes occurring in a pear callus (pyrus communis l., cv. beurré bosc) grown in close proximity to spatially separated undifferentiated homologous (pear) or heterologous (quince; cydonia oblonga mill., east malling clone c) cells in its neighboring environment. after a 7-day co-culture period, the presence of heterologous cells produced negative effects on the pear callus, whose relative weight increas ...201020190345
complete genome sequence of the fire blight pathogen erwinia amylovora cfbp 1430 and comparison to other erwinia spp.fire blight, caused by the enterobacterium erwinia amylovora, is a devastating disease of rosaceous plants that has global economic importance for apple and pear production and trade. the complete genome of e. amylovora cfbp 1430 was sequenced, annotated, and compared with the genomes of other erwinia spp. several singleton and shared features of the e. amylovora cfbp 1430 genome were identified that offer a first view into evolutionary aspects within the genus erwinia. comparative genomics iden ...201020192826
the multiple target use of spirodiclofen (envidor 240 sc) in ipm pomefruit in belgium.envidor 240 sc, active ingredient spirodiclofen is a broad spectrum acaricide acting via lipid biosynthesis inhibition (lbi) with no cross resistance to currently available acaricides and with additional insecticidal properties. envidor is positive ipm listed but in frame of resistance management limited to one application per season. in pear growing envidor constitutes an important tool in pear sucker control in a tandem strategy with abamectine. being totally selective on predatory bugs anthoc ...200920218531
the selectivity in the time of thiacloprid (calypso 480 sc) applied at preblossom on the further anthocoris nemoralis population build up in pears.predatory bugs (anthocoris sp.) are key predators of the pear sucker (psyllo pyri) in pear orchards. in order to enhance biological control it is important to verify if crop protection products applied against psylla pyri display potential short- or long-term detrimental effects on anthocoris populations. this study focuses on the impact of thiacloprid (calypso 480 sc), applied at preblossom for the control of the first generation pear sucker psylla pyri, on subsequent population build up of ant ...200920218534
baseline sensitivity of stemphylium vesicarium, the causal agent of pear brown spot, to boscalid. 200920222565
monitoring of the entomological diversity in a pesticide free orchard: investigation of the gembloux agricultural university conservatory.in orchards, the fruit production is currently oriented to integrated pest management. in order to develop efficient pest control strategy by using entomophagous beneficial and lowering the application of pesticides, sufficient knowledge of entomological populations is needed. here, a systematic monitoring of insect diversity and abundance in a conservatory orchard of gembloux was performed. combining yellow traps and visual observations, 19 pest and 34 beneficial families were identified among ...200920222593
cardiovascular images. chylotamponade: an unusual manifestation of gorham-stout syndrome. 201020233864
contribution of peroxisomes to secondary metabolism and pathogenicity in the fungal plant pathogen alternaria alternata.the filamentous fungus alternaria alternata includes seven pathogenic variants (pathotypes) which produce different host-selective toxins and cause diseases on different plants. the japanese pear pathotype produces the host-selective ak-toxin, an epoxy-decatrienoic acid ester, and causes black spot of japanese pear. previously, we identified four genes, akt1, akt2, akt3, and aktr, involved in ak toxin biosynthesis. akt1, akt2, and akt3 encode enzyme proteins with peroxisomal targeting signal typ ...201020348386
mice drinking goji berry juice (lycium barbarum) are protected from uv radiation-induced skin damage via antioxidant pathways.the goji berry, lycium barbarum, has long been recognised in traditional chinese medicine for various therapeutic properties based on its antioxidant and immune-modulating effects. this study describes the potential for orally consumed goji berry juice to alter the photodamage induced in the skin of mice by acute solar simulated uv (ssuv) irradiation. in skh:hr-1 hairless mice, 5% goji berry juice significantly reduced the inflammatory oedema of the sunburn reaction. dilutions of goji berry juic ...201020354657
erwinia piriflorinigrans sp. nov., a novel pathogen that causes necrosis of pear blossoms.eight erwinia strains, isolated from necrotic pear blossoms in valència, spain, were compared with reference strains of erwinia amylovora and erwinia pyrifoliae, both of which are pathogenic to species of pear tree, and to other species of the family enterobacteriaceae using a polyphasic approach. phenotypic analyses clustered the novel isolates into one phenon, distinct from other species of the genus erwinia, showing that the novel isolates constituted a homogeneous phenotypic group. rep-pcr p ...201120382791
increased catch of female codling moth (lepidoptera: tortricidae) in kairomone-baited clear delta traps.the relative performance of a clear delta trap baited with individual or combination sex pheromone and kairomone lures for codling moth, cydia pomonella l., was evaluated against three vertical clear pane and colored delta traps in field trials within apple, malus domestica (borkhausen). the clear caught significantly more moths of each sex than an orange delta trap when baited with ethyl (e,z)-2,4-decadienoate (pear ester) and acetic acid (pe+aa) and performed similarly to oil-coated pane traps ...201020388291
detection of european mountain ash ringspot-associated virus-specific rna and protein p3 in the pear leaf blister mite phytoptus pyri (eriophyidae).the means by which european mountain ash ringspot-associated virus (emarav), a minus-strand ssrna virus and the type member of the genus emaravirus, is naturally spread, is unknown. in attempts to identify an emarav vector, galls induced by the eriophyid mite phytoptus pyri were frequently found on infected leaves. by immunofluorescence microscopy, the presence of emarav nucleocapsid protein p3 was demonstrated in p. pyri individuals collected from diseased plants. furthermore, rt-pcr analysis o ...201020401694
determination of the folate content in cladodes of nopal (opuntia ficus indica) by microbiological assay utilizing lactobacillus casei (atcc 7469) and enzyme-linked immunosorbent assay.prickly pear cactus has been an important food source in mexico since ancient times due to its economical and ecological benefits and potential nutraceutical value. nevertheless, studies on the nutritional aspects and health benefits have been scarce. the purpose of this study was to assess, apparently for the first time, the folate contents of cladodes of nopal by a microbiological assay, using lactobacillus casei (atcc 7469) in extracts that were enzymatically treated to release the bound vita ...201020441169
evaluation of several rt-pcr primer pairs for the detection of apple stem pitting virus.detection of apple stem pitting virus (aspv) using rt-pcr based methods was studied in infected apple and pear trees. three virus-specific primers (aspf1cp, aspf2cp, aspr3cp) were designed to target the most conservative regions of the coat protein gene of 10 virus isolates in poland and 7 other aspv sequences available in genbank. the suitability of the primer pairs aspf1cp-aspr3cp and aspf2cp-aspr3cp for detection of 19 virus isolates was checked. both new primer pairs initiated amplification ...201020447421
blood parasites of mound-building mouse, mus spicilegus petényi, 1882 (mammalia, rodentia).mound-building mice, mus spicilegus, were studied for the blood parasites in eastern slovakia, vicinity kechnec village near kosice town (kosická kotlina basin, 21 degrees 14' e, 48 degrees 33' n) during years 2002-2005. overall, 251 specimens were examined. the parasites were detected using microhematokrit centrifugation technique and on the giemsa's method stained blood smears and light microscopy. the parasites were found in 3.57% of specimens; 1.20% of mice were infected with bartonella sp., ...201020450010
virulence characteristics accounting for fire blight disease severity in apple trees and seedlings.the gram-negative bacterium erwinia amylovora is the causal agent of fire blight, the most destructive bacterial disease of rosaceous plants, including apple and pear. here, we compared the virulence levels of six e. amylovora strains (ea273, cfbp1367, ea581a, e2002a, e4001a, and hkn06p1) on apple trees and seedlings. the strains produced a range of disease severity, with hkn06p1 producing the greatest disease severity in every assay. we then compared virulence characteristic expression among th ...201020465409
gorham-stout syndrome of the petrous apex causing chronic cerebrospinal fluid leak.to describe the clinical course, diagnostic features, and treatment of a case of gorham-stout syndrome involving the petrous apex and causing chronic cerebrospinal fluid (csf) leak.201020502378
analyses of the secretomes of erwinia amylovora and selected hrp mutants reveal novel type iii secreted proteins and an effect of hrpj on extracellular harpin levels.summary erwinia amylovora is a plant pathogenic enterobacterium that causes fire blight disease of apple, pear and other rosaceous plants. a type iii (t3) secretion system, encoded by clustered, chromosomal hrp genes (hypersensitive response and pathogenicity), is essential for infection, but only a few proteins are known that are secreted through this pathway (the t3 'secretome'). we developed an efficient protocol for purification and concentration of extracellular proteins and used it to char ...200720507478
inhibition of the mechanical activity of mouse ileum by cactus pear (opuntia ficus indica, l, mill.) fruit extract and its pigment indicaxanthin.we investigated, using an organ bath technique, the effects of a hydrophilic extract from opuntia ficus indica fruit pulp (cactus fruit extract, cfe) on the motility of mouse ileum, and researched the extract component(s) responsible for the observed responses. cfe (10-320 mg of fresh fruit pulp equivalents/ml of organ bath) reduced dose-dependently the spontaneous contractions. this effect was unaffected by tetrodotoxin, a neuronal blocker, n(omega)-nitro-l-arginine methyl ester, a nitric oxide ...201020518499
characterization of microencapsulated pear ester, (2e,4z)-ethyl-2,4-decadienoate, a kairomonal spray adjuvant against neonate codling moth larvae.codling moth (cm), cydia pomonella (lepidoptera: tortricidae), is the key pest of apples, pears, and walnuts worldwide. the pear-derived kairomone, ethyl (2e,4z)-2,4-decadienoate, the pear ester (pe), evokes attraction and arrestment of cm larvae. microencapsulated pe formulation (pe-mec) enhances the control efficacy of insecticides when used as a spray adjuvant. characterization of the microencapsulated kairomone, including microcapsule size, concentrations, emission rates, and larval response ...201020527813
evaluation of tolerance to pierce's disease and botrytis in transgenic plants of vitis vinifera l. expressing the pear pgip gene.summary polygalacturonase-inhibiting proteins (pgips) are plant cell-wall proteins that specifically inhibit fungal endo-polygalacturonases (pgs) that contribute to the aggressive decomposition of susceptible plant tissues. the inhibition of fungal pgs by pgips suggests that pgips have a role in plant tolerance to fungal infections and this has been observed in transgenic plants expressing pgips. xylella fastidiosa, the causal agent of pierce's disease (pd) in grapevines, has genes that encode c ...200520565637
cytological and molecular analyses of non-host resistance of arabidopsis thaliana to alternaria alternata.summary when challenged with the necrotrophic fungal pathogen alternaria alternata japanese pear pathotype, all tested ecotypes of arabidopsis plants failed to show hypersensitive cell death, accumulation of detectable levels of reactive oxygen species or accumulation of phytoalexin. we operationally define a. alternata as a non-host pathogen for arabidopsis plants and show that the protection against a. alternata demonstrated in this study is a non-host penetration resistance. to characterize n ...200520565684
genome comparison of the epiphytic bacteria erwinia billingiae and e. tasmaniensis with the pear pathogen e. pyrifoliae.the genus erwinia includes plant-associated pathogenic and non-pathogenic enterobacteria. important pathogens such as erwinia amylovora, the causative agent of fire blight and e. pyrifoliae causing bacterial shoot blight of pear in asia belong to this genus. the species e. tasmaniensis and e. billingiae are epiphytic bacteria and may represent antagonists for biocontrol of fire blight. the presence of genes that are putatively involved in virulence in e. amylovora and e. pyrifoliae is of special ...201020565991
survey of susceptibility to abamectin of pear psylla (hemiptera: psyllidae) in northern italy.the pear psylla, cacopsylla pyri l. (hemiptera: psyllidae), is a relevant pest of pear, pyrus communis l., trees in emilia-romagna region (northern italy). the susceptibility to the insecticide abamectin was evaluated at different times of the year on c. pyri populations undergoing different control strategies within conventional, integrated, and organic farms. the tests performed were the egg spray and the topic and dip bioassay on adults. the larval mortality was evaluated by dip bioassay on t ...201020568628
the dspb/f protein of erwinia amylovora is a type iii secretion chaperone ensuring efficient intrabacterial production of the hrp-secreted dspa/e pathogenicity factor.summary erwinia amylovora is a gram-negative bacterium responsible for fire blight, a necrotic disease affecting plants of the rosaceae family. e. amylovora virulence is dependent on a functional type iii secretion system. to date, four proteins have been shown to travel through this secretion system: hrpn, hrpw, hrpa, and dspa/e. next to dspa/e, dspb/f encodes a small acidic protein sharing features similar to those of type iii secretion chaperones described in animal systems. here, we show tha ...200220569339
characterization of homologues of the apple proliferation immunodominant membrane protein gene from three related phytoplasmas.summary homologues of the immunodominant membrane protein gene from apple proliferation (ap) phytoplasma have been cloned and sequenced for three further members of the ap subclade, namely european stone fruit yellows, peach yellow leaf roll and a european isolate of pear decline (pd). the putative translation products of all three were similar in size to that of ap and all had a transmembrane region towards the n-terminus and a large c-terminal hydrophilic domain, probably held on the outside o ...200320569369
from host recognition to t-dna integration: the function of bacterial and plant genes in the agrobacterium-plant cell interaction.abstract agrobacterium tumefaciens and its related species, a. rhizogenes and a. vitis, are the only known bacterial pathogens which 'genetically invade' host plants and stably integrate part of their genetic material into the host cell genome. thus, a. tumefaciens has evolved as a major tool for plant genetic engineering. furthermore, this unique process of interkingdom dna transfer has been utilized as a model system for studies of its underlying biological events, such as intercellular signal ...200020572967
erwinia amylovora: the molecular basis of fireblight disease.summary taxonomy: bacteria; proteobacteria; gamma subdivision; order enterobacteriales; family enterobacteriaceae; genus erwinia. microbiological properties: gram-negative, motile rods. related species:e. carotovora (soft-rot diseases), e. chrysanthemi (soft-rot diseases), e. (pantoea) stewartii (stewart's wilt of corn), e. (pantoea) herbicola (epiphyte). host range: affects rosaceous plants, primarily members of the pomoideae. economically important hosts are apple and pear. the commercial impl ...200020572979
phytophthora infestans enters the genomics era.summary phytophthora infestans, cause of late-blight, is the most devastating disease of potato world-wide. recent years have seen a dramatic intensification in molecular biological studies of p. infestans, including the development of novel tools for transformation and gene silencing and the resources for genetical, transcriptional and physical mapping of the genome. this review will focus on the increasing efforts to use these resources to discover the genetic bases of pathogenicity, avirulenc ...200120573013
[hypoglycemic effect of extracts of cactus pear fruit polysaccharide in rats].to study the hypoglycemic effect and mechanism of the extracts of cactus pear fruit polysaccharide (cpfp) in diabetic rats induced by streptozotocin (stz).201020575420
thirty-year follow-up of patient with gorham disease (massive osteolysis) treated with hip arthroplasty.gorham disease (also known as massive osteolysis or disappearing bone disease) is an exceedingly rare, peculiar entity of uncertain cause and unpredictable prognosis. there is no proven mode of therapy or consensus on treatment. we present 30-year follow-up on a patient successfully treated with resection, total hip arthroplasty, and radiation with no recurrence of disease. our observations in this case suggest that surgical treatment and reconstruction can lead to good long-term function and di ...201120580190
in vitro susceptibility of erwinia amylovora (burrill) winslow et. al. to citrus maxima essential oil.regulatory constraints and environmental and human health concerns have promoted the search for alternative bio-control strategies of fire blight, a destructive disease of rosaceous plants which produces serious losses in apple and pear orchards all over the world. the aim of this study was to establish the antimicrobial activity of citrus maxima essential oil against erwinia amylovora. an agar diffusion method was used for the screening of the inhibitory effect of citrus maxima essential oil on ...200920583476
a panel of real-time pcr assays for specific detection of three phytoplasmas from the apple proliferation group.we report here on the development of combination of assays for fast, reliable, specific and sensitive detection and discrimination of 'candidatus phytoplasma mali', 'ca. p. prunorum' and 'ca. p. pyri' from the 16sr-x (apple proliferation - ap) group. these phytoplasmas are causal agents of diseases of fruit trees within the family rosaceae, namely apple proliferation (ap), european stone fruit yellows (esfy) and pear decline (pd). the designed panel of assays uses taqman minor groove binder prob ...201020600822
morphological, pathological and molecular variability in botryodiplodia theobromae (botryosphaeriaceae) isolates associated with die-back and bark canker of pear trees in punjab, india.thirteen isolates of botryodiplodia theobromae collected from pear varieties grown in various regions of punjab were studied for morphological, pathological and molecular characterization. the mycelial growth of b. theobromae isolates was classified as fluffy or depressed, uniform to irregular and cottony white turning to black. colony growth rate varied from 19.1 to 24.9 mm per day. pycnidia were produced either on the edge, centered or scattered on petri dishes after 20 to 34 days of incubatio ...201020603807
first report of effective and feasible treatment of multifocal lymphangiomatosis (gorham-stout) with bevacizumab in a child. 201020605931
some factors influencing patulin production by penicillium expansum in pome fruits.the objective of our study was to examine the effects of penicillium expansum on patulin production in relation to isolates, species and cultivar type, incidence and severity of decay. in addition, patulin production at different incubation times and its diffusion were also investigated. these factors were evaluated in pome fruits inoculated with p. expansum and kept at 20 °c for short periods of time.201020623709
detection of babesia vogeli in stray cats of metropolitan bangkok, thailand.the combination of a rapidly growing stray animal population and the lack of animal control in bangkok has resulted in a unique opportunity to evaluate the potential role of companion animals as sentinels and reservoirs of infectious diseases, including several of those caused by vector-borne parasites. the purpose of this study was to determine the prevalence and factors associated with the distribution of babesia species infections among stray cats in bangkok. blood samples were collected from ...201020638794
ultrastructural analysis of chlamydia pneumoniae in the alzheimer's brain.we recently reported identification of the bacterium chlamydia pneumoniae in affected brain regions of patients with alzheimer's disease (ad) (balin et al., 1998). in this report, we extend those initial observations to demonstrate that, in addition to the frequently described, standard morphological forms of the organism, pleiomorphic forms are also present in the ad brain. all ad and control brain tissues examined were verified to be pcr-positive and negative, respectively, for the organism. d ...199920671799
inhibitory effects of antioxidant reagent in reactive oxygen species generation and penetration of appressoria of alternaria alternata japanese pear pathotype.in the japanese pear pathotype of alternaria alternata, h2o2 is generated solely from penetration pegs and not from other portions of subcuticular hyphae within the pectin layers of host leaves. a close association between h2o2 generation and fungal aggressiveness is expected because the pegs are important for fungal penetration into the host epidermis. to determine the potential role of reactive oxygen species in microbial pathogenicity, we studied the inhibitory effects of the antioxidant reag ...201020701480
two neutral variants segregating at the gametophytic self-incompatibility locus of european pear (pyrus communis l.) (rosaceae, pyrinae).extensive survey of the s-locus diversity of plant species with rnase-based gametophytic self-incompatibility has failed to identify neutral variation segregating within s-allele specificities. although this is the expected result according to population genetics theory, it conflicts with recent models of s-allele evolution, which suggest that new specificities might arise by a continuous process of subtle changes that individually do not alter the specificity of the s-genes, but whose cumulativ ...201020701704
cornelian cherry (cornus mas l.) juices as a source of minerals in human diet.the aim of this study was to determine the mineral content of cornelian cherry (cornus mas l.), as this fruit and its preservatives may be considered as important nutritional supplements. potassium (k), calcium (ca), sodium (na), iron (fe), zinc (zn), manganese (mn), and copper (cu) were present in cornelian cherry juice as measured by atomic absorption. compared to other juices obtained from plum, pear, and apple, cornelian cherry juice contained high levels of ca, reaching 10-fold higher (323 ...201020706938
[gorham-stout syndrome (gss) with fulminant aseptic osteonecrosis of the shoulder].we report here a case of the rare gorham-stout syndrome (gss) of the humerus. gss is a disease in the course of which spontaneous idiopathic osteolysis occurs. we describe a case of a 46-year-old business economist who suffered an inadequate trauma and within 3 weeks developed self-limited idiopathic osteolysis of the left humerus head, also affecting the rotator cuff. this could be diagnosed especially on the basis of histopathological findings. hereupon we indicated operative repair by means o ...201020714707
a rare cause of dyspnea due to chylothorax.we report the case of an 8-year-old boy who presented to the emergency department of another hospital and was referred to our pediatric intensive care unit with dyspnea and tachypnea of recent onset. the diagnosis of massive chylothorax with mediastinal shift was made on chest radiographs and ct scan. initial investigations revealed no definite cause for the chylothorax. on later radiography and ct imaging with 3d surface rendering, a marked bone loss of the left ribs, clavicle, and shoulder joi ...201020721678
sensory profile of a model energy drink with varying levels of functional ingredients-caffeine, ginseng, and taurine.energy drinks have increased in popularity in recent years due to the claimed energy boost provided by functional ingredients. a multitude of functional ingredients have been utilized; however, there is limited research on their sensory effects in energy drink formulations. a 13-member descriptive analysis panel was conducted to investigate the effects on the sensory and rheological properties of 3 common functional ingredients-caffeine, ginseng, and taurine-in a noncarbonated model energy drink ...201020722948
gorham-stout disease.gorham-stout disease is a rare disease of unknown etiology. it is characterized by spontaneous excessive replacement of bone by proliferative non-neoplastic thin-walled lymphatic and/or blood vessels. histology shows positive stain for the lymphatic endothelial marker lyve-1 (lymphatic vascular endothelial hyaluronan receptor-1) and many lymphatic growth factors (pdgf-bb, vegf-c, vegfr-3). patients may present with localized pain and/or weakness and radiographic evidence of massive osteolysis in ...201020727303
host range, biological variation, and phylogenetic diversity of eutypa lata in california.the objectives of this study were to investigate the host range of eutypa lata in the major grape-growing regions in california and to analyze the phenotypic variation and phylogenetic diversity of e. lata isolates. perithecia of e. lata were found on grapevines, in apricot, almond, cherry, apple, and pear tree orchards, and on ornamentals (oleander) and native plant species (california buckeye, big leaf maple, and willow). multigene phylogenies of ribosomal dna internal transcribed spacer, β-tu ...201020839940
mechanistically compatible mixtures of bacterial antagonists improve biological control of fire blight of pear.mixtures of biological control agents can be superior to individual agents in suppressing plant disease, providing enhanced efficacy and reliability from field to field relative to single biocontrol strains. nonetheless, the efficacy of combinations of pseudomonas fluorescens a506, a commercial biological control agent for fire blight of pear, and pantoea vagans strain c9-1 or pantoea agglomerans strain eh252 rarely exceeds that of individual strains. a506 suppresses growth of the pathogen on fl ...201120839962
control of fire blight by pseudomonas fluorescens a506 and pantoea vagans c9-1 applied as single strains and mixed inocula.the biological control agents pseudomonas fluorescens a506 and pantoea vagans c9-1 were evaluated individually and in combination for the suppression of fire blight of pear or apple in 10 field trials inoculated with the pathogen erwinia amylovora. the formulation of pathogen inoculum applied to blossoms influenced establishment of the pathogen and the efficacy of biological control. pantoea vagans c9-1 suppressed fire blight in all five trials in which the pathogen was applied as lyophilized ce ...201020839963
[relationship between ige antibodies to recombinant allergens rbet v 1 and rbet v 2 and food causing oral allergy syndrome in cases of birch-pollen allergy].persons allergic to birch pollen often report oral and pharyngeal hypersensitivity to fruit and vegetables, such as apples and peaches due to immunological cross-reactivity, or oral allergy syndrome (oas) sometimes accompanied by systemic reaction. such cross-reactive antigen reactions involve bet v 1, the main birch-pollen allergen, and bet v 2, birch-pollen profilin. we evaluated the food/antigen relationship.201020845708
human motion tracking by temporal-spatial local gaussian process experts.human pose estimation via motion tracking systems can be considered as a regression problem within a discriminative framework. it is always a challenging task to model the mapping from observation space to state space because of the high-dimensional characteristic in the multimodal conditional distribution. in order to build the mapping, existing techniques usually involve a large set of training samples in the learning process which are limited in their capability to deal with multimodality. we ...201120851794
comparison of lures loaded with codlemone and pear ester for capturing codling moths, cydia pomonella, in apple and pear orchards using mating disruption.abstract studies were conducted in apple, malus domestica borkhausen and pear, pyrus communis l. (rosales: rosaceae), orchards to evaluate the attractiveness of grey halobutyl septa loaded with 1 (l2) and 10 (mega) mg of codlemone, 8e, 10e-dodecadien-1-ol, 3 mg of pear ester, ethyl (e,z)-2,4-decadienoate (da2313), and 3 mg of pear ester plus 3 mg of codlemone (combo) to adult codling moth, cydia pomonella (l.) (lepidoptera: tortricidae). all studies were conducted in orchards treated with pherom ...201020883133
diversity of the bacterial community in the surface soil of a pear orchard based on 16s rrna gene analysis.a cultivation-independent approach based on polymerase chain reaction (pcr)-amplified partial small subunit rrna genes was used to characterize bacterial populations in the surface soil of a commercial pear orchard consisting of different pear cultivars during two consecutive growing seasons. pyrus communis l. cvs blanquilla, conference, and williams are among the most widely cultivated cultivars in europe and account for the majority of pear production in northeastern spain. to assess the heter ...201020890846
genetic analysis of streptomycin-resistant (sm(r)) strains of erwinia amylovora suggests that dissemination of two genotypes is responsible for the current distribution of sm(r) e. amylovora in michigan.streptomycin-resistant (sm(r)) strains of the fire blight pathogen erwinia amylovora were first isolated in southwest michigan in 1991. since that time, resistant strains have progressed northward to other apple-producing regions in the state. a total of 98.7% of sm(r) strains isolated between 2003 and 2009 in michigan harbored the stra-strb genes on transposon tn5393. stra and strb encode phosphotransferase enzymes that modify streptomycin to a nonbactericidal form. mutational resistance to str ...201120923367
evaluation of kasugamycin for fire blight management, effect on nontarget bacteria, and assessment of kasugamycin resistance potential in erwinia amylovora.the emergence and spread of streptomycin-resistant strains of erwinia amylovora in michigan has necessitated the evaluation of new compounds effective for fire blight control. the aminoglycoside antibiotic kasugamycin (ks) targets the bacterial ribosome and is particularly active against e. amylovora. the efficacy of ks formulated as kasumin 2l for control of fire blight was evaluated in six experiments conducted over four field seasons in our experimental orchards in east lansing, mi. blossom b ...201120923369
microfluidic immunosensor with micromagnetic beads coupled to carbon-based screen-printed electrodes (spces) for determination of botrytis cinerea in tissue of fruits.a wide range of plant species, including economically important crops such as vegetables, ornamentals, bulbs, and fundamentally fruits, can be affected by gray mold caused by the fungal pathogen botrytis cinerea . this paper describes the development of a microfluidic immunosensor with micromagnetic beads (mmbs) coupled to carbon-based screen-printed electrodes (spces) for the rapid and sensitive quantification of b. cinerea in apple (red delicious), table grape (pink moscatel), and pear (willia ...201020931959
Displaying items 1001 - 1100 of 1726