Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| astaxanthin dirhamnoside, a new astaxanthin derivative produced by a radio-tolerant bacterium, sphingomonas astaxanthinifaciens. | 2009 | 19557030 | |
| a new concept for reduction of diffuse contamination by simultaneous application of pesticide and pesticide-degrading microorganisms. | pesticide residues and their transformation products are frequently found in groundwater and surface waters. this study examined whether adding pesticide-degrading microorganisms simultaneously with the pesticide at application could significantly reduce diffuse contamination from pesticide use. degradation of the phenoxyacetic acid herbicides mcpa (4-chloro-2-methylphenoxyacetic acid) and 2,4-d (2,4-dichlorophenoxyacetic acid) was studied in soil microcosm experiments after simultaneous sprayin ... | 2010 | 19557524 |
| analysis of genes encoding the 2,4-dichlorophenoxyacetic acid-degrading enzyme from sphingomonas agrestis 58-1. | a 2,4-dichlorophenoxy acetic acid (2,4-d)-degrading bacterium, strain 58-1, was newly isolated from soil samples collected in the fukuoka prefecture, japan, and grown on an enrichment culture medium containing 2,4-d as the sole carbon source. phylogenic analysis identified strain 58-1 as sphingomonas agrestis. in 2,4-d degraders, classes i, ii, and iii inherit the tfda, cada, and tfdaalpha genes, respectively, and the results from degenerate-pcr indicated that this strain belongs to the class ii ... | 2009 | 19577193 |
| association between contaminated faucets and colonization or infection by nonfermenting gram-negative bacteria in intensive care units in taiwan. | this study was designed to determine the strength of the association between the isolation of nonfermentative gram-negative bacilli (nfgnb) from tap water faucet aerators and the prevalence of colonization or infection of patients in intensive care units (icus). surveillance cultures were obtained during a 4-month period from 162 faucet aerators located in seven different icus. the prevalence of colonization or infection of icu patients with nfgnb was determined by prospective surveillance durin ... | 2009 | 19587299 |
| study of the degradation activity and the strategies to promote the bioavailability of phenanthrene by sphingomonas paucimobilis strain 20006fa. | the present study describes the phenanthrene-degrading activity of sphingomonas paucimobilis 20006fa and its ability to promote the bioavailability of phenanthrene. s. paucimobilis 20006fa was isolated from a phenanthrene-contaminated soil microcosm. the strain was able to grow in liquid mineral medium saturated with phenanthrene as the sole carbon source, showing high phenanthrene elimination (52.9% of the supplied phenanthrene within 20 days). the accumulation of 1-hydroxy-2-naphthoic acid and ... | 2010 | 19609598 |
| [effect of the inoculant strain sphingomonas paucimobilis 20006fa on the bacterial composition of a phenanthrene-degrading consortium]. | the effect of the inoculant strain sphingomonas paucimobilis 20006fa on the bacterial composition of a phenanthrene-degrading consortium obtained from a pristine soil in sequencing batch cultures was studied. inoculated (f200+1) and non-inoculated (f200) phenanthrene-degrading consortia, were obtained. bacterial diversity of consortia was studied at cultivable (phenotype and genotype characterization) and non-cultivable (pcr-dgge) levels. during the successive cultures, a loss in the phenanthren ... | 2009 | 19623894 |
| sphingomonas hankookensis sp. nov., isolated from wastewater. | a gram-negative, non-motile, rod-shaped bacterial strain, odn7(t), was isolated from a wastewater treatment plant in korea, and its taxonomic position was investigated by use of a polyphasic taxonomic approach. strain odn7(t) grew optimally at around ph 7.5 and 30 degrees c and in the presence of 0-0.5 % (w/v) nacl. phylogenetic analysis based on 16s rrna gene sequences showed that strain odn7(t) fell within the cluster comprising species of the genus sphingomonas, clustering with sphingomonas p ... | 2009 | 19625422 |
| quinone-mediated decolorization of sulfonated azo dyes by cells and cell extracts from sphingomonas xenophaga. | the effects of various quinone compounds on the decolorization rates of sulfonated azo dyes by sphingomonas xenophaga qyy were investigated. the results showed that anthraquinone-2-sulfonate (aqs) was the most effective redox mediator and aqs reduction was the rate-limited step of aqs-mediated decolorization of sulfonated azo dyes. based on aqs biological toxicity tests, it was assumed that aqs might enter the cells and kill them. in the cytoplasmic extracts from strain qyy, aqs more effectively ... | 2009 | 19634426 |
| [sphingomonas sp.: an important microbial resource for biopolymer synthesis]. | the genus sphingomonas was established in 1990. sphingomonas spp. synthesize sphingans, structurally related biopolymers such as gellan, welan and diutan. at present, only gellan is applied widely in foods and pharmaceuticals. the economic value of other sphingans has not been well explored, and related research of sphingans still remains limited. in the present review, we address the latest taxonomy developments of sphingomonas, details about structure, characteristics and biosynthetic pathway ... | 2009 | 19637560 |
| sphingomonas changbaiensis sp. nov., isolated from forest soil. | bacterial strain v2m44(t) was isolated from forest soil from the changbai mountains, heilongjiang province, china. cells of strain v2m44( t) were gram-negative, rod-shaped and 0.3-0.4 mum in diameter and 1.5-2.5 mum long. the cells were strictly aerobic and were motile by means of peritrichous flagella. growth occurred at 20-33 degrees c (optimum, 29-31 degrees c), at ph 5-8 (optimum, ph 6.0-7.0) and in the presence of 0-0.1 % (w/v) nacl (optimum, 0.05 %). strain v2m44(t) contained c(17 : 1 )ome ... | 2010 | 19656932 |
| gellan gum: a new biomaterial for cartilage tissue engineering applications. | gellan gum is a polysaccharide manufactured by microbial fermentation of the sphingomonas paucimobilis microorganism, being commonly used in the food and pharmaceutical industry. it can be dissolved in water, and when heated and mixed with mono or divalent cations, forms a gel upon lowering the temperature under mild conditions. in this work, gellan gum hydrogels were analyzed as cells supports in the context of cartilage regeneration. gellan gum hydrogel discs were characterized in terms of mec ... | 2010 | 19658177 |
| biodegradation of polycyclic aromatic hydrocarbons by sphingomonas sp. enhanced by water-extractable organic matter from manure compost. | the effectiveness of in-situ bioremediation of polycyclic aromatic hydrocarbons (pahs) may be inhibited by their low aqueous solubility and strong absorption to soil constituents. the aim of this research was to evaluate the effect of water-extractable organic matter (weom) from manure compost on the biodegradation of various pahs. the aqueous solubilities of pahs including phenanthrene, pyrene and benzo[a]pyrene under different concentrations of weom from cow manure compost were initially evalu ... | 2009 | 19660784 |
| sphingomonas histidinilytica sp. nov., isolated from a hexachlorocyclohexane dump site. | a gram-negative, non-spore-forming, cream-coloured bacterial strain, um2(t), was isolated from an open hexachlorocyclohexane (hch) dump site at ummari village in lucknow, india. data generated from a polyphasic approach including phenotypic, genotypic and chemotaxonomic analyses confirmed that strain um2(t) belonged to the genus sphingomonas. the highest similarity found to the 16s rrna gene sequence of strain um2(t) was 99.4 %, with sphingomonas wittichii dsm 6014(t), whereas the dna-dna relate ... | 2010 | 19666786 |
| genetic diversity of gamma-hexachlorocyclohexane-degrading sphingomonads isolated from a single experimental field. | to isolate gamma-hexachlorocyclohexane (gamma-hch)-degrading bacteria from a single field and to examine their genetic diversity. | 2009 | 19674290 |
| co-ordinated regulation of two divergent promoters through higher-order complex formation by the lysr-type regulator thnr. | the genes required for tetralin biodegradation by sphingomonas macrogolitabida strain tfa are clustered in two divergent and closely linked operons. thnr, a lysr-type regulator, activates transcription from each operon in response to tetralin. the regulatory thnr gene is co-transcribed with the catabolic genes thnc, thna3 and thna4, resulting in positive autoregulation. thnr binds with different affinity to two primary binding sites, designated b and c, in the intervening region between the two ... | 2009 | 19682246 |
| mutational study of the role of n-terminal amino acid residues in tetrachlorohydroquinone reductive dehalogenase from sphingomonas sp. ug30. | this research presents the first extensive mutational study of n-terminal amino acids necessary for activity of a bacterial zeta class glutathione transferase (gst). our studies on ug30 tetrachlorohydroquinone reductive dehalogenase (pcpc) revealed that, similar to other zeta class gsts, n-terminal ser and cys residues play critical roles in glutathione binding and their mutation results in functional and structural changes to pcpc. mutation of the n-terminal ser and cys residues decreased the a ... | 2009 | 19682569 |
| vertical distribution and anaerobic biodegradation of polycyclic aromatic hydrocarbons in mangrove sediments in hong kong, south china. | the vertical distribution of polycyclic aromatic hydrocarbons (pahs) at different sediment depths, namely 0-2 cm, 2-4 cm, 4-6 cm, 6-10 cm, 10-15 cm and 15-20 cm, in one of the most contaminated mangrove swamps, ma wan, hong kong was investigated. it was the first time to study the intrinsic potential of deep sediment to biodegrade pahs under anaerobic conditions and the abundance of electron acceptors in sediment for anaerobic degradation. results showed that the total pahs concentrations (summa ... | 2009 | 19683792 |
| diversity, abundance, and consistency of microbial oxygenase expression and biodegradation in a shallow contaminated aquifer. | the diversity of rieske dioxygenase genes and short-term temporal variability in the abundance of two selected dioxygenase gene sequences were examined in a naphthalene-rich, coal tar waste-contaminated subsurface study site. using a previously published pcr-based approach (s. m. ní chadhain, r. s. norman, k. v. pesce, j. j. kukor, and g. j. zylstra, appl. environ. microbiol. 72:4078-4087, 2006) a broad suite of genes was detected, ranging from dioxygenase sequences associated with rhodococcus a ... | 2009 | 19700556 |
| bacterial growth and biofilm production on pyrene. | enrichment cultures inoculated with arctic soil yielded a biofilm that grew on pyrene and phenanthrene. in a 60-day period, the biofilm degraded 20 microg ml(-1) pyrene or 39 microg ml(-1) phenanthrene. single colonized pyrene crystals (approximately 1.5x0.75x0.35 mm) yielded 10(11) culturable heterotrophs and 10(5) biofilm propagules. analysis of ribosomal intergenic spacers identified six phylotypes in a clone library from the pyrene biofilm. analysis of 16s rrna gene sequences indicated that ... | 2002 | 19709207 |
| cloning and characterization of phosphomannose isomerase from sphingomonas chungbukensis dj77. | phosphomannose isomerase (pmi) catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate in the extracellular polysaccharide (eps) synthesis pathway. the gene encoding pmi in sphingomonas chungbukensis dj77 was cloned and expressed in e. coli. the pmi gene is 1,410 nucleotides long and the deduced amino acid sequence shares high homology with other bifunctional proteins that possess both pmi and gdp-mannose pyrophosphorylase (gmp) activities. the sequence analysis of pmi reve ... | 2009 | 19712590 |
| influence of phenanthrene and fluoranthene on the degradation of fluorene and glucose by sphingomonas sp. strain lb126 in chemostat cultures. | abstract since bacteria degrading polycyclic aromatic hydrocarbon compounds (pahs) in polluted soils are generally exposed to mixtures of pahs, we examined the influence of simple pah mixtures on the degradation activity of sphingomonas sp. strain lb126. fluorene serves as sole carbon and energy source for the strain lb126 and phenanthrene and fluoranthene are cometabolically degraded by this species. chemostat cultures of the strain lb126 were used to study a potential inhibiting effect of phen ... | 2003 | 19719587 |
| crystallization and preliminary x-ray crystallographic analysis of phok, an extracellular alkaline phosphatase from sphingomonas sp. bsar-1. | alkaline phosphatases (aps) are widely distributed from microbes to humans and are involved in several important biological processes such as phosphate nutrition, signal transduction and pathogenesis. alkaline phosphatases are also useful in various industrial applications and in recombinant dna technology. a new ap enzyme from sphingomonas sp. strain bsar-1, termed phok, has been shown to be useful in uranium bioprecipitation. phok was expressed, purified and crystallized. the crystals belonged ... | 2009 | 19724132 |
| circular dichroism spectra, optical rotations and absolute configurations of cis-dihydrodiol metabolites of quinoline and derivatives: the role of the nitrogen atom. | the absolute configurations of cis-dihydrodiol bacterial metabolites derived from quinoline substrates and their quinolinium salts have been determined by comparison of experimental and calculated chiroptical data (circular dichroism spectra and optical rotations). the role of intramolecular h-bonding and other interactions in the preferred conformations has been investigated, including the effect of protonation of several cis-dihydrodiols. the presence of a strong intramolecular oh...n bond in ... | 2009 | 19750495 |
| random transposon vectors puttns for the markerless integration of exogenous genes into gram-negative eubacteria chromosomes. | a set of random transposon vectors puttns that facilitates the markerless integration of new functions into the chromosome of gram-negative bacteria has been developed. the vectors, which are derived from mini-tn5 transposons, are located on a r6k-based suicide delivery plasmid that provides the is50(r) transposase tnp gene in cis, but they are external to the mobile element. the vectors' conjugal transfer to recipients is mediated by rp4 mobilization functions in the donor. internal to the mini ... | 2009 | 19778558 |
| previously uncultured beta-proteobacteria dominate in biologically active granular activated carbon (bac) filters. | bacteria colonizing bac filters used in drinking water purification from lake water were characterized by morphology, physiological tests, whole cell protein profiles and plfa (phospholipid fatty acid) composition, and identified by partial 16s rrna gene sequencing. epifluorescence revealed prothecate bacteria to dominate in bac. the majority of the isolates belonged to order burkholderiales of beta-proteobacteria, a few to comamonadaceae but the majority to an undescribed family and the related ... | 2009 | 19783028 |
| community proteogenomics reveals insights into the physiology of phyllosphere bacteria. | aerial plant surfaces represent the largest biological interface on earth and provide essential services as sites of carbon dioxide fixation, molecular oxygen release, and primary biomass production. rather than existing as axenic organisms, plants are colonized by microorganisms that affect both their health and growth. to gain insight into the physiology of phyllosphere bacteria under in situ conditions, we performed a culture-independent analysis of the microbiota associated with leaves of so ... | 2009 | 19805315 |
| bromhidrosis induced by sphingomonas paucimobilis: a case report. | bromhidrosis is a clinical disorder characterized by excessive or abnormal foul axillary odour due to the interaction of apocrine glands with micro-organisms which causes a serious personal and social handicap for affected people. we present the case of a 50-year-old caucasian female with bromhidrosis. the patient referred that this symptom had begun two months previously. her past treatments included antibacterial soap, topical antibacterial agents and perfumes, but none of these relieved the p ... | 2009 | 19822101 |
| rapid degradation of phenanthrene by using sphingomonas sp. gy2b immobilized in calcium alginate gel beads. | the strain sphingomonas sp. gy2b is a high efficient phenanthrene-degrading strain isolated from crude oil contaminated soils that displays a broad-spectrum degradation ability towards pahs and related aromatic compounds. this paper reports embedding immobilization of strain gy2b in calcium alginate gel beads and the rapid degradation of phenanthrene by the embedded strains. results showed that embedded immobilized strains had high degradation percentages both in mineral salts medium (msm) and 8 ... | 2009 | 19826557 |
| characterization of an isoproturon mineralizing bacterial culture enriched from a french agricultural soil. | the phenylurea herbicide isoproturon, 3-(4-isopropylphenyl)-1,1-dimethylurea (ipu), was found to be rapidly mineralized by a bacterial culture isolated from an agricultural soil regularly exposed to ipu. molecular analysis of the bacterial culture by dna fingerprinting, cloning and sequencing of the 16s rrna genes revealed that it consisted of six different members among whom the dominant was related to sphingomonas sp. six bacterial strains belonging to genera ancylobacter, pseudomonas, stenotr ... | 2009 | 19836052 |
| characterisation of hexane-degrading microorganisms in a biofilter by stable isotope-based fatty acid analysis, fish and cultivation. | the hexane-degrading bacterial community of a biofilter was characterised by a combination of stable isotope-based phospholipid fatty acid analyses, fluorescence in situ hybridisation and cultivation. about 70 bacterial strains were isolated from a full-scale biofilter used for treatment of hexane containing waste gas of an oil mill. the isolation approach led to 16 bacterial groups, which were identified as members of the alpha-, beta- and gammaproteobacteria, actinobacteria and firmicutes. thr ... | 2010 | 19847422 |
| sorption to humic acids enhances polycyclic aromatic hydrocarbon biodegradation. | the hypothesis that humic acids (ha) can act as carriers of polycyclic aromatic hydrocarbon (pah) compounds and provide bacteria with pah fluxes beyond those obtained by diffusion of the water-dissolved fraction of these poorly soluble chemicals was tested. controlled degradation experiments using microcrystalline phenanthrene and sphingomonas sp. lh162 were performed without ha and in the presence of two ha concentrations. in the absence of ha, phenanthrene was steadily present at the maximum d ... | 2009 | 19848123 |
| contribution of microbial activity to carbon chemistry in clouds. | the biodegradation of the most abundant atmospheric organic c1 to c4 compounds (formate, acetate, lactate, succinate) by five selected representative microbial strains (three pseudomonas strains, one sphingomonas strain, and one yeast strain) isolated from cloud water at the puy de dôme has been studied. experiments were first conducted under model conditions and consisted of a pure strain incubated in the presence of a single organic compound. kinetics showed the ability of the isolates to degr ... | 2010 | 19854931 |
| [genetic diversity of cultivable bacteria of dominant plants in a potassium mine tailing of nanjing]. | plant rhizosphere bacteria play an important role in biogeochemical cycles. microbial diversity of cultivable bacteria from soil-plant-mineral system in a mine tailing was assessed. | 2009 | 19873749 |
| abundance of novel and diverse tfda-like genes, encoding putative phenoxyalkanoic acid herbicide-degrading dioxygenases, in soil. | phenoxyalkanoic acid (paa) herbicides are widely used in agriculture. biotic degradation of such herbicides occurs in soils and is initiated by alpha-ketoglutarate- and fe2+-dependent dioxygenases encoded by tfda-like genes (i.e., tfda and tfdaalpha). novel primers and quantitative kinetic pcr (qpcr) assays were developed to analyze the diversity and abundance of tfda-like genes in soil. five primer sets targeting tfda-like genes were designed and evaluated. primer sets 3 to 5 specifically ampli ... | 2010 | 19880651 |
| tetralin-induced and thnr-regulated aldehyde dehydrogenase and beta-oxidation genes in sphingomonas macrogolitabida strain tfa. | a new cluster of genes has been found downstream of the previously identified thna2 gene. the gene products are similar to nonacylating aldehyde dehydrogenases (thng) and to proteins representing a complete beta-oxidation pathway (thnh to thnp). thng has a nonacylating nad-dependent pimelic semialdehyde dehydrogenase activity that renders pimelic acid a seven-carbon dicarboxylic acid. for further metabolism via beta-oxidation, pimelic acid could be acylated by a constitutive acyl coenzyme a (acy ... | 2010 | 19897762 |
| crystallization and preliminary crystallographic analysis of gallate dioxygenase desb from sphingobium sp. syk-6. | gallate dioxygenase (desb) from sphingobium sp. syk-6, which belongs to the type ii extradiol dioxygenase family, was purified and crystallized using the hanging-drop vapour-diffusion method. two crystal forms were obtained. the form i crystal belonged to space group c2, with unit-cell parameters a = 136.2, b = 53.6, c = 55.1 angstrom, beta = 112.8 degrees, and diffracted to 1.6 angstrom resolution. the form ii crystal belonged to space group p2(1), with unit-cell parameters a = 56.2, b = 64.7, ... | 2009 | 19923743 |
| stable high level expression of the violacein indolocarbazole anti-tumour gene cluster and the streptomyces lividans amya gene in e. coli k12. | previous studies showed that when ppsx-vioabcde was used to transform e. coli k12 dh5alpha the strain retained the plasmid even after 100 generations of unselected growth but produced a low level of the anti-tumour antibiotic violacein. markedly higher levels of violacein synthesis were obtained from e. coli k12 dh5alpha puc18-vioabcde and sphingomonas sp. jmp4092 ppsx-vioabcde. unfortunately, both strains were extremely unstable regardless of presence or absence of antibiotic selection to retai ... | 2010 | 19962399 |
| using orthogonal design to determine optimal conditions for biodegradation of phenanthrene in mangrove sediment slurry. | in the present paper, the effects of four factors, each at three levels, on biodegradation of phenanthrene, a 3-ring pah, in contaminated mangrove sediment slurry were investigated using the orthogonal experimental design. the factors and levels were (i) sediment types (clay loam, clayey and sandy); (ii) different inoculums (sphingomonas sp., a mixture of sphingomonas sp. and mycobacterium sp., and without inoculum); (iii) presence of other pahs (fluorene, pyrene, and none); and (iv) different s ... | 2010 | 20022168 |
| ecotoxicity by the biodegradation of alkylphenol polyethoxylates depends on the effect of trace elements. | the bacteria sphingomonas sp. strain bsn22, isolated from bean fields, degraded octylphenol polyethoxylates (opeo(n)) to octylphenol (op) under aerobic conditions. this biodegradation mechanism proceeded by the following two-step degradation process: (1) degradation of opeo(n) to octylphenol triethoxylate (opeo(3)), (2) degradation from opeo(3) to op via octylphenoxy acetic acid (opec(1)). the chemical structure of opec(1) was confirmed by analysis using (18)o-labeled water. quantitative studies ... | 2010 | 20025273 |
| isolation and characterization of bacteria resistant to metallic copper surfaces. | metallic copper alloys have recently attracted attention as a new antimicrobial weapon for areas where surface hygiene is paramount. currently it is not understood on a molecular level how metallic copper kills microbes, but previous studies have demonstrated that a wide variety of bacteria, including escherichia coli, staphylococcus aureus, and clostridium difficile, are inactivated within minutes or a few hours of exposure. in this study, we show that bacteria isolated from copper alloy coins ... | 2010 | 20048058 |
| commensal microbiota and cd8+ t cells shape the formation of invariant nkt cells. | commensal bacteria play an important role in formation of the immune system, but the mechanisms involved are incompletely understood. in this study, we analyze cd1d-restricted invariant nkt (inkt) cells in germfree mice and in two colonies of c57bl/6 mice termed conventional flora and restricted flora (rf), stably bearing commensal microbial communities of diverse but distinct composition. in germfree mice, inkt cells were moderately reduced, suggesting that commensal microbiota were partially r ... | 2010 | 20048124 |
| isolation and characterization of novel bisphenol-a--degrading bacteria from soils. | one hundred and seven soil samples were collected from various places in japan, and their bisphenol-a (bpa, 2,2-bis(4-hydroxyphenyl) propane) degradative capacities were evaluated. eighty-five soil samples possessed bpa degradative capacities, and 26 bacterial strains could be isolated as bpa-degrading bacterium. sequence analysis of their 16s rrna genes indicated that 22 isolates belonged to proteobacteria groups, and three of four gram-positive bacterial strains, ya27, no13, and no15, were cla ... | 2009 | 20055221 |
| cloning, expression, purification, crystallization and preliminary crystallographic studies of ugdg, an udp-glucose dehydrogenase from sphingomonas elodea atcc 31461. | gellan gum, a commercial gelling agent produced by sphingomonas elodea atcc 31461, is a high-value microbial exopolysaccharide. udp-glucose dehydrogenase (ugd; ec 1.1.1.22) is responsible for the nad-dependent twofold oxidation of udp-glucose to udp-glucuronic acid, one of the key components for gellan biosynthesis. s. elodea atcc 31461 ugd, termed ugdg, was cloned, expressed, purified and crystallized in native and semet-derivatized forms in hexagonal and tetragonal space groups, respectively; ... | 2010 | 20057075 |
| selective enumeration strategies for brevundimonas diminuta from drinking water. | brevundimonas diminuta is used as a control organism for validating the efficiency of water filtration systems. since these protocols use nonselective growth media, heterotrophic plate count bacteria (hpcs) indigenous to the water distribution system may interfere with b. diminuta enumeration, thus leading to inaccurate assessment of the filter's microbial reduction capability. this could negatively impact public health as unsafe drinking water may be produced. this study was conducted to evalua ... | 2010 | 20087629 |
| polyphasic bacterial community analysis of an aerobic activated sludge removing phenols and thiocyanate from coke plant effluent. | biological purification processes are effective tools in the treatment of hazardous wastes such as toxic compounds produced in coal coking. in this study, the microbial community of a lab-scale activated sludge system treating coking effluent was assessed by cultivation-based (strain isolation and identification, biodegradation tests) and culture-independent techniques (sequence-aided t-rflp, taxon-specific pcr). the results of the applied polyphasic approach showed a simple microbial community ... | 2010 | 20093025 |
| phylogenetic diversity and antimicrobial activities of bryozoan-associated bacteria isolated from mediterranean and baltic sea habitats. | to date, only a small number of investigations covering microbe-bryozoa associations have been carried out. most of them have focused on a few bryozoan species and none have covered the antibacterial activities of associated bacteria. in the current study, the proportion and phylogenetic classification of bryozoan-associated bacteria with antimicrobial properties were investigated. twenty-one specimens of 14 different bryozoan species were collected from several sites in the baltic and the medit ... | 2010 | 20153592 |
| chemoenzymatic synthesis of chiral 2,2'-bipyridine ligands and their n-oxide derivatives: applications in the asymmetric aminolysis of epoxides and asymmetric allylation of aldehydes. | a series of enantiopure 2,2'-bipyridines have been synthesised from the corresponding cis-dihydrodiol metabolites of 2-chloroquinolines. several of the resulting hydroxylated 2,2'-bipyridines were found to be useful chiral ligands for the asymmetric aminolysis of meso-epoxides leading to the formation of enantioenriched amino alcohols (-->84% ee). n-oxide and n,n'-dioxide derivatives of these 2,2'-bipyridines, including separable atropisomers, have been synthesised and used as enantioselective o ... | 2010 | 20165798 |
| c, n, and h isotope fractionation of the herbicide isoproturon reflects different microbial transformation pathways. | the fate of pesticides in the subsurface is of great interest to the public, industry, and regulatory authorities. compound-specific isotope analysis (csia) is a promising tool complementary to existing methods for elucidating pesticide degradation reactions. here, we address three different initial biotransformation reactions of the phenylurea herbicide isoproturon (3-(4-isopropylphenyl)-1,1-dimethylurea) in pure culture experiments with bacterial and fungal strains. when analyzing isotopic cha ... | 2010 | 20184312 |
| biofilm formation on reverse osmosis membranes is initiated and dominated by sphingomonas spp. | the initial formation and spatiotemporal development of microbial biofilm layers on surfaces of new and clean reverse osmosis (ro) membranes and feed-side spacers were monitored in situ using flow cells placed in parallel with the ro system of a full-scale water treatment plant. the feed water of the ro system had been treated by the sequential application of coagulation, flocculation, sand filtration, ultrafiltration, and cartridge filtration processes. the design of the flow cells permitted th ... | 2010 | 20190090 |
| anticoagulating activities of low-molecular weight fuco-oligosaccharides prepared by enzymatic digestion of fucoidan from the sporophyll of korean undaria pinnatifida. | in spite of their potential as biologically active compounds, the high molecular mass and viscous natures of fucoidans have hampered their applications especially as a therapeutic agent. herein the fucoidan-degrading enzyme activities were partially purified from the cultured cells of sphingomonas paucimobilis pf-1 mainly by ammonium sulfate precipitation. this enzyme preparation degraded fucoidans from the korean undaria pinnatifida sporophyll into several low-molecular weight fuco-oligosacchar ... | 2010 | 20191353 |
| seasonal and regional diversity of maple sap microbiota revealed using community pcr fingerprinting and 16s rrna gene clone libraries. | an arbitrary primed community pcr fingerprinting technique based on capillary electrophoresis was developed to study maple sap microbial community characteristics among 19 production sites in québec over the tapping season. presumptive fragment identification was made with corresponding fingerprint profiles of bacterial isolate cultures. maple sap microbial communities were subsequently compared using a representative subset of 13 16s rrna gene clone libraries followed by gene sequence analysis. ... | 2010 | 20202776 |
| biodegradation studies and sequencing of microcystin-lr degrading bacteria isolated from a drinking water biofilter and a fresh water lake. | the presence of microcystin-lr -degrading bacteria in an active anthracite biofilter and in lake mead, nevada was investigated. four bacterial isolates from enrichment culture were identified using 16s rrna analysis. microcystin biodegradation tests were performed with both, the enrichment cultures and the respective isolates, using microcystin alone and acetate as carbon sources. a newly recognized microcystin-degrading bacterium, morganella morganii, was isolated from the biofilter and from la ... | 2010 | 20219514 |
| annual variations in the diversity, viability, and origin of airborne bacteria. | the presence of bacteria in aerosols has been known for centuries, but information on their identity and role in dispersing microbial traits is still limited. this study monitored the airborne bacterial community during an annual survey using samples collected from a 25-m tower near the baltic sea coast. the number of cfu was estimated using agar plates while the most probable number (mpn) of viable bacteria was estimated using dilution-to-extinction culturing assays (dcas). the mpn and cfu valu ... | 2010 | 20228096 |
| a novel selective growth medium-pcr assay to isolate and detect sphingomonas in environmental samples. | sphingomonas species can be found ubiquitously in the environment and can be frequently found in surface biofilms. some sphingomonas strains are well known for metabolizing complex organic pollutants but some are opportunistic human pathogens. despite the importance of the sphingomonas species, a reliable system to isolate this group of bacteria from the environment has not been developed. in this study, a combined streptomycin-piperacillin selective growth medium/polymerase chain reaction (pcr) ... | 2010 | 20359508 |
| culture-independent techniques applied to food industry water surveillance--a case study. | culture-independent techniques were used for the detection of pathogenic bacteria in drinking water at potentially critical control points along the production lines at a german dairy company and a spanish dry cured ham company. denaturing gradient gel electrophoresis (dgge) was used to describe bacterial population shifts indicating biological instability in the drinking water samples. autochthonous bacteria were identified by sequencing the excised dgge dna bands. more specifically, real-time ... | 2010 | 20363042 |
| the genes coding for the conversion of carbazole to catechol are flanked by is6100 elements in sphingomonas sp. strain xldn2-5. | carbazole is a recalcitrant compound with a dioxin-like structure and possesses mutagenic and toxic activities. bacteria respond to a xenobiotic by recruiting exogenous genes to establish a pathway to degrade the xenobiotic, which is necessary for their adaptation and survival. usually, this process is mediated by mobile genetic elements such as plasmids, transposons, and insertion sequences. | 2010 | 20368802 |
| sphingomonas glacialis sp. nov., a psychrophilic bacterium isolated from alpine glacier cryoconite. | a non-motile, rod-shaped, yellow bacterium, designated c16y(t), was isolated from alpine glacier cryoconite. cells behaved gram-positively, were aerobic and psychrophilic (good growth at 1-25 °c). phylogenetic analysis based on 16s rrna gene sequences showed that strain c16y(t) was related to the genus sphingomonas and had highest 16s rrna gene sequence similarity with sphingomonas oligophenolica jcm 12082(t) (97.6 %) and sphingomonas echinoides dsm 1805(t) (97.2 %). dna-dna hybridization demo ... | 2011 | 20382784 |
| the effect of structure and a secondary carbon source on the microbial degradation of chlorophenoxy acids. | pseudomonas putida, aspergillus niger, bacillus subtilis, pseudomonas fluorescens, sphingomonas herbicidovorans and rhodococcus rhodochrous growing on glucose in a medium containing one of three chlorophenoxy acids at a concentration of 0.1 g l(-1) (clofibric acid, (r)-2-(4-chloro-2-methylphenoxy)propionic acid (mecoprop or mcpp) and 4-chloro-2-methylphenoxyacetic acid (mcpa)) degraded these compounds to varying degrees; from nonmeasurable to almost complete removal. these results with the addit ... | 2010 | 20392474 |
| adhesion and biofilm formation on polystyrene by drinking water-isolated bacteria. | this study was performed in order to characterize the relationship between adhesion and biofilm formation abilities of drinking water-isolated bacteria (acinetobacter calcoaceticus, burkholderia cepacia, methylobacterium sp., mycobacterium mucogenicum, sphingomonas capsulata and staphylococcus sp.). adhesion was assessed by two distinct methods: thermodynamic prediction of adhesion potential by quantifying hydrophobicity and the free energy of adhesion; and by microtiter plate assays. biofilms w ... | 2010 | 20405208 |
| biofilm formation in environmental bacteria is influenced by different macromolecules depending on genus and species. | the formation of biofilms by diverse bacteria isolated from contaminated soil and groundwater on model substrata with different surface properties was assessed in a multifactorial screen. diverse attachment phenotypes were observed as measured by crystal violet dye retention and confocal laser scanning microscopy (clsm). bulk measurements of cell hydrophobicity had little predictive ability in determining whether biofilms would develop on hydrophobic or hydrophilic substrata. therefore selected ... | 2010 | 20406292 |
| [strategy of proportioning of bacteria capable for conversion of polyaromatic compounds]. | 2010 | 20411664 | |
| biofilms formed on humic substances: response to flow conditions and carbon concentrations. | stream biofilms are exposed to dynamic conditions of flow velocity and organic carbon availability. thus, the aim of this study was to investigate the response of biofilms formed with and without humic substances (hss) to an increase in flow velocity (0.04-0.10 ms(-1)) and hss concentration (9.7+/-1.0 to 19.8+/-0.4 mgl(-1) c). the highest amount of biofilm, measured as volatile suspended solids and total countable cells, was observed at 0.10 m s(-1) without hss. the bacterial community compositi ... | 2010 | 20413305 |
| sphingomonas paucimobilis bacteremia in humans: 16 case reports and a literature review. | sphingomonas paucimobilis is a glucose-nonfermenting gram-negative bacillus that is widely distributed in both natural environment and hospitals. various infections in humans have been reported, but most have been limited to sporadic case reports. the aim of this study was to describe the clinical characteristics and manifestations of s. paucimobilis bacteremia. we also reviewed the literature on s. paucimobilis bacteremia. | 2010 | 20434121 |
| sphingomonas paucimobilis: a persistent gram-negative nosocomial infectious organism. | non-fermenting gram-negative bacilli create a significant problem in clinical settings, being the most widespread cause of nosocomial infections. they are opportunistic pathogens that take advantage of underlying conditions and diseases. sphingomonas paucimobilis, a non-fermenting gram-negative bacillus, is regarded as of minor clinical significance; however, many instances of infections with this organism can be found in the literature. infections include bacteraemia/septicaemia caused by conta ... | 2010 | 20434794 |
| sphingomonas humi sp. nov., isolated from soil. | a gram-negative, non-motile, non-spore-forming, small, orange, rod-shaped bacterium was isolated from soil in south korea and characterized to determine its taxonomic position. phylogenetic analysis based on 16s rrna gene sequence examination revealed that strain pb323(t) belongs to the family sphingomonadaceae. the highest degree of sequence similarity was found with sphingomonas kaistensis pb56(t) (98.9%), followed by sphingomonas astaxanthinifaciens tdma-17(t) (98.3%). chemotaxonomic characte ... | 2010 | 20437147 |
| inhibition of the plp-dependent enzyme serine palmitoyltransferase by cycloserine: evidence for a novel decarboxylative mechanism of inactivation. | cycloserine (cs, 4-amino-3-isoxazolidone) is a cyclic amino acid mimic that is known to inhibit many essential pyridoxal 5'-phosphate (plp)-dependent enzymes. two cs enantiomers are known; d-cycloserine (dcs, also known as seromycin) is a natural product that is used to treat resistant mycobacterium tuberculosis infections as well as neurological disorders since it is a potent nmda receptor agonist, and l-cycloserine (lcs) is a synthetic enantiomer whose usefulness as a drug has been hampered by ... | 2010 | 20445930 |
| isolation of phylogenetically diverse nonylphenol ethoxylate-degrading bacteria and characterization of their corresponding biotransformation pathways. | most nonylphenol ethoxylate (npeo)-degrading isolates have been assigned to gamma-proteobacteria, which is different from the results acquired by using molecular ecological techniques. to better understand the environmental fate of npeos, bacterial isolation strategy characterized by the use of gellan gum as a gelling reagent and a low concentration of target carbon source were used to isolate phylogenetically diverse npeo-degrading bacteria from activated sludge, and the biotransformation pathw ... | 2010 | 20457466 |
| degradation of natural estrogen and identification of the metabolites produced by soil isolates of rhodococcus sp. and sphingomonas sp. | five bacterial strains capable of utilizing 17beta-estradiol (e2) and estrone (e1) were isolated from soil samples. using their morphological and physiological features and 16s rdna sequences, we classified these isolates into two groups: group a (rhodococcus sp. strains ed6, ed7, and ed10) and group b (sphingomonas sp. strains ed8 and ed9). all isolates used e2 and e1 as the sole carbon sources and showed high e1 and e2 degradation activities. in all strains, more than 50% of 0.8 mg of e1 or e2 ... | 2010 | 20471597 |
| multisubstrate kinetics of pah mixture biodegradation: analysis in the double-logarithmic plot. | the proposed method of kinetic analysis of aqueous-phase biodegradation of polycyclic aromatic hydrocarbons (pah) mixture presupposes representation of kinetic curves for each pair of mixture components, s(x) and s(y), in double-logarithmic coordinates (ln s(x); ln s(y)). if pah mixture conversion corresponds to the multisubstrate model with a common active site, then the graphs in double-logarithmic coordinates are straight lines with the angular coefficients equal to the ratio of respective fi ... | 2011 | 20490624 |
| sphingomonas rubra sp. nov., isolated from bioreactor wastewater. | a gram-reaction-negative, rod-shaped, motile, neutrophilic bacterium, designated strain bh3(t), was isolated from wastewater of a sequential batch reactor treating wastewater taken from a leather plant. the isolate grew in 0-8 % (w/v) nacl, at ph 6-9 and at 4-45 °c. chemotaxonomic analysis showed that strain bh3(t) had characteristics typical of members of the genus sphingomonas, such as the presence of sphingolipids, q-10 and 2-hydroxymyristic acid and the absence of 3-hydroxy fatty acids. the ... | 2011 | 20511462 |
| atp modulates the growth of specific microbial strains. | the regulatory function of extracellular atp (exatp) in bacteria is unknown, but recent studies have demonstrated exatp induced enhanced secondary metabolite production and morphological differentiation in streptomyces coelicolor. the growth of streptomyces coelicolor, however, was unaffected by exatp, although changes in growth are common phenotypes. to identify bacteria whose growth is altered by exatp, we measured exatp-induced population changes in fast-growing microbes and actinomycetes in ... | 2011 | 20512647 |
| inverting the enantioselectivity of p450pyr monooxygenase by directed evolution. | we report the first example of directed evolution of a p450 monooxygenase with inverted enantioselectivity for asymmetric biohydroxylation. the biohydroxylation product of the best mutant 1af4a has an ee of 83% (r) compared to the wild type's ee of 43% (s). | 2010 | 20520890 |
| isolation and identification of persistent chlorinated organophosphorus flame retardant-degrading bacteria. | tris(2-chloroethyl) and tris(1,3-dichloro-2-propyl) phosphates are chlorinated persistent flame retardants that have recently emerged as environmental pollutants. two bacterial strains that can degrade the compounds when they are the sole phosphorus sources have been isolated and identified as members of the sphingomonads. the strains can be useful for the bioremediation of environments contaminated with these compounds. | 2010 | 20525857 |
| a 17beta-estradiol-utilizing bacterium, sphingomonas strain kc8: part i - characterization and abundance in wastewater treatment plants. | a 17beta-estradiol-utilizing bacterium, sphingomonas strain kc8, was characterized in terms of its utilization kinetics toward 17beta-estradiol, estrone, and testosterone. the maximum specific substrate utilization rates (q(m)) are 0.37, 0.50, and 0.17 mg-substrate/mg-protein/day for 17beta-estradiol, estrone, and testosterone, respectively. the half-velocity constants (k(s)) are 1.9 mg/l for 17beta-estradiol, 2.7 mg/l for estrone, and 2.4 mg/l for testosterone. strain kc8 can grow on testostero ... | 2010 | 20527759 |
| functional assembly of bacterial communities with activity for the biodegradation of an organophosphorus pesticide in the rape phyllosphere. | although most pesticides sprayed on terrestrial plants remain on their leaf surfaces, the relationship between leaf-associated microbial populations and pesticide degradation remains unclear. here we examined changes in the bacterial community composition in the rape phyllosphere after treatment with dichlorvos, an organophosphorus pesticide. results indicate that the bacterial community showed marked changes after treatment. we evaluated the rate of dichlorvos degradation by a natural microbial ... | 2010 | 20529133 |
| a new extreme environment for aerobic anoxygenic phototrophs: biological soil crusts. | biological soil crusts improve the health of arid or semiarid soils by enhancing water content, nutrient relations and mechanical stability, facilitated largely by phototrophic microorganisms. until recently, only oxygenic phototrophs were known from soil crusts. a recent study has demonstrated the presence of aerobic representatives of earth's second major photosynthetic clade, the evolutionarily basal anoxygenic phototrophs. three canadian soil crust communities yielded pink and orange aerobic ... | 2010 | 20532732 |
| the serine palmitoyltransferase from sphingomonas wittichii rw1: an interesting link to an unusual acyl carrier protein. | serine palmitoyltransferase (spt) catalyses the first step in the de novo biosynthesis of sphingolipids (sls). it uses a decarboxylative claisen-like condensation reaction to couple l-serine with palmitoyl-coa to generate a long-chain base product, 3-ketodihydrosphingosine. sls are produced by mammals, plants, yeast, and some bacteria, and we have exploited the complete genome sequence of sphingomonas wittichii to begin a complete analysis of bacterial sphingolipid biosynthesis. here, we describ ... | 2010 | 20578000 |
| mutational studies of the peptidoglycan hydrolase flgj of sphingomonas sp. strain a1. | the flagellar protein flgj, a member of glycoside hydrolase family 73, has n- and c-terminal domains that are responsible for flagellar rod assembly and peptidoglycan hydrolysis, respectively. the crystal structure of the c-terminal domain of sph1045 (sph1045-c), the flgj from sphingomonas sp. strain a1, showed a long cleft formed by two lobes, alpha and beta. in this study, seven site-specific mutants of residues in the cleft were prepared and analyzed. enzyme activity was reduced most signific ... | 2010 | 20586063 |
| identification of two flavin monooxygenases from an effluent treatment plant sludge metagenomic library. | oxygenases are useful for the production of many industrially important molecules. screening of an effluent treatment plant (etp) sludge metagenomic library identified two clones encoding proteins, b1 and b2, with similarity to putative flavin monooxygenases from mesorhizobium loti and sphingomonas wittichi, respectively. the deduced amino acid sequences show only 20% identity, but both have a paired rossman fold and a flavin monooxygenase (fmo) motif. b1 and b2 appear to be members of the flavi ... | 2010 | 20591656 |
| diversity and physiology of polyhydroxyalkanoate-producing and -degrading strains in microbial mats. | photosynthetic microbial mats are sources of microbial diversity and physiological strategies that reflect the physical and metabolic interactions between their resident species. this study focused on the diversity and activity of polyhydroxyalkanoate-producing and -degrading bacteria and their close partnership with cyanobacteria in an estuarine and a hypersaline microbial mat. the aerobic heterotrophic population was characterized on the basis of lipid biomarkers (respiratory quinones, sphingo ... | 2010 | 20618859 |
| metabolic profiles and phylogenetic diversity of microbial communities from chlorinated pesticides contaminated sites of different geographical habitats of india. | to study the microbial communities in three sites contaminated with chlorinated pesticides and evaluation of dehydrodechlorinase (lina) gene variants involved in gamma-hexachlorocyclohexane (γ-hch, lindane) degradation. | 2010 | 20629804 |
| construction of an artificial microalgal-bacterial consortium that efficiently degrades crude oil. | four oil component-degrading bacteria and one oil-tolerant microalgae, scenedesmus obliquus gh2, were used to construct an artificial microalgal-bacterial consortium for crude-oil degradation. the bacterial strains included sphingomonas gy2b and burkholderia cepacia gs3c, along with a mixed culture, named gp3, containing pseudomonas gp3a and pandoraea pnomenusa gp3b. gy2b could only degrade polycyclic aromatic hydrocarbons, gs3c was able to degrade aliphatic chain hydrocarbons, and gp3 could uti ... | 2010 | 20638971 |
| nature of bioavailability of dna-intercalated polycyclic aromatic hydrocarbons to sphingomonas sp. | the nature of bioavailability of dna-intercalated pahs in aqueous solution was investigated. the degradability of different pahs including anthracene, phenanthrene and pyrene by sphingomonas sp. was not inhibited even at a high dna concentration of 2%. the dna was stable against the pah-degrader as indicated by the unchanged electrophoresis gel chromatograms after treatment. this shows that a structural change in the polymer is not necessary for the release of pahs. partitioning experiments usin ... | 2010 | 20646737 |
| in vivo protection provided by a synthetic new alpha-galactosyl ceramide analog against bacterial and viral infections in murine models. | alpha-galactosyl ceramide (α-galcer) has been known to bind to the cd1d receptor on dendritic cells and activate invariant natural killer t (inkt) cells, which subsequently secrete t-helper-cell 1 (th1) and th2 cytokines, which correlate with anti-infection activity and the prevention of autoimmune diseases, respectively. α-galcer elicits the secretion of these two cytokines nonselectively, and thus, its effectiveness is limited by the opposing effects of the th1 and th2 cytokines. reported here ... | 2010 | 20660669 |
| an optical microbial biosensor for detection of methyl parathion using sphingomonas sp. immobilized on microplate as a reusable biocomponent. | organophosphorus pesticides such as methyl parathion have been widely used in the field of agriculture for insect pest control. these pesticides and their degradation products cause environmental pollution and ecological problem. with a view to monitor these pesticides biosensors are being developed. a bacterium sphingomonas sp. from field soil has been isolated and identified in our laboratory that hydrolyzes the methyl parathion upto a chromophoric product, p-nitrophenol (pnp). pnp can be dete ... | 2010 | 20685107 |
| molecular identification of unsaturated uronate reductase prerequisite for alginate metabolism in sphingomonas sp. a1. | in sphingomonas sp. a1, alginate is degraded by alginate lyases to its constituent monosaccharides, which are nonenzymatically converted to an alpha-keto acid, namely, 4-deoxy-l-erythro-5-hexoseulose uronic acid (deh). the properties of the deh-metabolizing enzyme and its gene in strain a1 were characterized. in the presence of alginate, strain a1 cells inducibly produced an nadph-dependent deh reductase (a1-r) in their cytoplasm. molecular cloning of the enzyme gene indicated that a1-r belonged ... | 2010 | 20685299 |
| ninety-day oral toxicity study of rhamsan gum, a natural food thickener produced from sphingomonas atcc 31961, in crl:cd(sd)igs rats. | this study was designed to evaluate and characterize any subchronic toxicity of rhamsan gum, a polysaccharide produced from sphingomonas strain atcc 31961, when administered to both sexes of crl:cd(sd)igs rats at dietary levels of 0 (control), 0.5, 1.5, and 5.0% (10 rats/sex/group). during the study, the treatment had no adverse effects on clinical signs, survival, body weights and food and water consumption, or on findings of urinalysis, ophthalmology, hematology, or blood biochemistry. examina ... | 2010 | 20686336 |
| effects of pyrene and fluoranthene on the degradation characteristics of phenanthrene in the cometabolism process by sphingomonas sp. strain pheb4 isolated from mangrove sediments. | cometabolism has been suggested as an attractive approach to enhance the degradation rates of high-molecular-weight polycyclic aromatic hydrocarbons (pahs). however, the effects of these recalcitrant pahs on the degradation characteristics of low-molecular-weight pahs are largely unknown. this study was conducted to investigate the effects of pyrene (pyr) and fluoranthene (fla) on the degradation characteristics of phenanthrene (phe) in the cometabolism process by sphingomonas sp. strain pheb4 i ... | 2010 | 20708757 |
| sphingomonas xinjiangensis sp. nov., isolated from the desert of xinjiang, china. | a gram-negative, aerobic, motile and rod-shaped sphingomonas-like bacterium, strain 10-1-84t, was isolated from a sand sample collected from the desert of xinjiang, china, and was subjected to a polyphasic taxonomic approach. the strain contained q-10 as the major predominant ubiquinone and c18 : 1omega7c, c16 : 0, c14:0 2-oh and c 16:1omega7c as the major fatty acids. the polyamine pattern predominantly contains sym-homospermidine. the main polar lipids were sphingoglycolipid, phosphatidylcholi ... | 2010 | 20817834 |
| phaseolus vulgaris seed-borne endophytic community with novel bacterial species such as rhizobium endophyticum sp. nov. | the bacterial endophytic community present in different phaseolus vulgaris (bean) cultivars was analyzed by 16s ribosomal rna gene sequences of cultured isolates derived from surface disinfected roots and immature seeds. isolated endophytes from tissue-macerates belonged to over 50 species in 24 different genera and some isolates from acinetobacter, bacillus, enterococcus, nocardioides, paracoccus, phyllobacterium, and sphingomonas seem to correspond to new lineages. phytate solubilizing bacteri ... | 2010 | 20822874 |
| isolation of mutant alginate lyases with cleavage specificity for di-guluronic acid linkages. | alginates are commercially valuable and complex polysaccharides composed of varying amounts and distribution patterns of 1-4-linked β-d-mannuronic acid (m) and α-l-guluronic acid (g). this structural variability strongly affects polymer physicochemical properties and thereby both commercial applications and biological functions. one promising approach to alginate fine structure elucidation involves the use of alginate lyases, which degrade the polysaccharide by cleaving the glycosidic linkages t ... | 2010 | 20826807 |
| genome sequence of the dioxin-mineralizing bacterium sphingomonas wittichii rw1. | pollutants such as polychlorinated biphenyls and dioxins pose a serious threat to human and environmental health. natural attenuation of these compounds by microorganisms provides one promising avenue for their removal from contaminated areas. over the past 2 decades, studies of the bacterium sphingomonas wittichii rw1 have provided a wealth of knowledge about how bacteria metabolize chlorinated aromatic hydrocarbons. here we describe the finished genome sequence of s. wittichii rw1 and major fi ... | 2010 | 20833805 |
| electrochemical and microbiological characterization of paper mill biofilms. | biofilm samples collected from inside and outside the press and former sections of paper machines in a northwestern ontario paper mill for a period of 2 years were characterized microbiologically and electrochemically. bacterial community profiling was done using polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) and selected bacterial isolates were identified using 16s rdna analysis. the bacterial community showed the presence of proteobacteria, firmicutes, and actinob ... | 2010 | 20835930 |
| a non-invasive fluorescent staining procedure allows confocal laser scanning microscopy based imaging of mycobacterium in multispecies biofilms colonizing and degrading polycyclic aromatic hydrocarbons. | to study the micro scale interactions of mycobacterium with bacteria belonging to other genera by means of confocal laser scanning microscopy (clsm), a procedure was developed to non-invasively and fluorescently stain mycobacterium without compromising the signal produced by commonly used fluorescent reporter genes. the procedure makes use of the commercial non-specific nucleic acid stain syto62 and was optimized to efficiently stain mycobacterium cells in suspensions and biofilms. the staining ... | 2010 | 20854847 |
| coenzyme q(10) production directly from precursors by free and gel-entrapped sphingomonas sp. zute03 in a water-organic solvent, two-phase conversion system. | in a water-organic solvent, two-phase conversion system, coq(10) could be produced directly from solanesol and para-hydroxybenzoic acid (phb) by free cells of sphingomonas sp. zute03 and coq(10) concentration in the organic solvent phase was significantly higher than that in the cell. coq(10) yield reached a maximal value of 60.8 mg l(-1) in the organic phase and 40.6 mg g(-1)-dcw after 8 h. coq(10) also could be produced by gel-entrapped cells in the two-phase conversion system. soybean oil and ... | 2011 | 20857286 |
| detecting variants with metabolic design, a new software tool to design probes for explorative functional dna microarray development. | microorganisms display vast diversity, and each one has its own set of genes, cell components and metabolic reactions. to assess their huge unexploited metabolic potential in different ecosystems, we need high throughput tools, such as functional microarrays, that allow the simultaneous analysis of thousands of genes. however, most classical functional microarrays use specific probes that monitor only known sequences, and so fail to cover the full microbial gene diversity present in complex envi ... | 2010 | 20860850 |
| [diversity of bacterial community in rhizosphere soils under effects of continuously planting burley tobacco]. | by using t-rflp approach, this paper studied the dynamic changes of bacterial community in the rhizosphere soils under continuously planting burley tobacco for 1, 2 and 4 years. with the increasing of continuous planting years, the shannon index and margalef index of bacterial community in the rhizosphere soils decreased after an initial increase. after 4-year continuous planting, the diversity of the bacterial community decreased significantly, and the community structure became simple. the sim ... | 2010 | 20879533 |
| sphingolipid signaling in fungal pathogens. | sphingolipid involvement in infectious disease is a new and exciting branch of research. various microbial pathogens have been shown to synthesize their own sphingolipids and some have evolved methods to "hijack" host sphingolipids for their own use. for instance, sphingomonas species are bacterial pathogens that lack the lipopolysaccharide component typical but instead contain glycosphingolipids (kawahara 1991, 2006). in terms of sphingolipid signaling and function, perhaps the best-studied gro ... | 2010 | 20919658 |
| comparison of maldi tof with conventional identification of clinically relevant bacteria. | a completely new approach to diagnose microbial agents at least one day earlier based on mass spectrometric analysis becomes possible in the microbiology laboratory: maldi tof: matrix-assisted laser desorption/ionisation time-of-flight. comparison between results of the new procedure with those obtained by conventional testing is mandatory. | 2010 | 20924806 |
| temporal changes in sphingomonas and mycobacterium populations in mangrove sediments contaminated with different concentrations of polycyclic aromatic hydrocarbons (pahs). | the change in community diversity and structure of the indigenous, dominant, polycyclic aromatic hydrocarbon (pah)-degrading bacterial genera, sphingomonas and mycobacterium, due to contamination in the environment is not very well known. a combination of pcr-dgge with specific primers and a cultivation-dependent microbiological method was used to detect different populations of sphingomonas and mycobacterium in mangrove sediments. the structure of the entire bacterial community (including sphin ... | 2011 | 20926106 |