Publications

panstrongylus geniculatus and four other species of triatomine bug involved in the trypanosoma cruzi enzootic cycle: high risk factors for chagas' disease transmission in the metropolitan district of caracas, venezuela.	chagas' disease is caused by the protozoan trypanosoma cruzi and is autochthonous to the americas. its distribution depends on triatomine bugs that are responsible for the transmission of the disease. in 2005, we reported the presence of panstrongylus geniculatus as a risk for chagas' disease transmission in caracas and neighboring areas. three massive oral outbreaks occurred in the following years. here we report the results of a 7-year study on triatomine species found in the metropolitan dist ...	2014	25532708
adipokinetic hormone signalling system in the chagas disease vector, rhodnius prolixus.	neuropeptides and their g protein-coupled receptors are widespread throughout metazoa and in several cases, clear orthologues can be identified in both protostomes and deuterostomes. one such neuropeptide is the insect adipokinetic hormone (akh), which is related to the mammalian gonadotropin-releasing hormone. akh has been studied extensively and is known to mobilize lipid, carbohydrates and proline for energy-consuming activities such as flight. in order to determine the possible roles for thi ...	2015	25545120
the effect of convection on infrared detection by antennal warm cells in the bloodsucking bug rhodnius prolixus.	previous work revealed that bloodsucking bugs can discriminate between oscillating changes in infrared (ir) radiation and air temperature (t) using two types of warm cells located in peg-in-pit sensilla and tapered hairs (zopf lm, lazzari cr, tichy h. j neurophysiol 111: 1341-1349, 2014). these two stimuli are encoded and discriminated by the response quotient of the two warm cell types. ir radiation stimulates the warm cell in the peg-in-pit sensillum more strongly than that in the tapered hair ...	2015	25609113
genetically modifying the insect gut microbiota to control chagas disease vectors through systemic rnai.	technologies based on rna interference may be used for insect control. sustainable strategies are needed to control vectors of chagas disease such as rhodnius prolixus. the insect microbiota can be modified to deliver molecules to the gut. here, escherichia coli ht115(de3) expressing dsrna for the rhodnius heme-binding protein (rhbp) and for catalase (cat) were fed to nymphs and adult triatomine stages. rhbp is an egg protein and cat is an antioxidant enzyme expressed in all tissues by all devel ...	2015	25675102
the impact of climate change on the geographical distribution of two vectors of chagas disease: implications for the force of infection.	chagas disease, caused by the parasite trypanosoma cruzi, is the most important vector-borne disease in latin america. the vectors are insects belonging to the triatominae (hemiptera, reduviidae), and are widely distributed in the americas. here, we assess the implications of climatic projections for 2050 on the geographical footprint of two of the main chagas disease vectors: rhodnius prolixus (tropical species) and triatoma infestans (temperate species). we estimated the epidemiological implic ...	2015	25688019
a kazal-type inhibitor is modulated by trypanosoma cruzi to control microbiota inside the anterior midgut of rhodnius prolixus.	the triatomine insect, rhodnius prolixus, is a vector of trypanosoma cruzi, a protozoan parasite that causes chagas disease. the parasite must overcome immune response and microbiota to develop inside the midgut of triatomines. in this study, we expressed, purified and characterized a kazal-type inhibitor from the midgut of r. prolixus, named rpti, which may be involved in microbiota - t. cruzi interactions. the qpcr showed that the rpti transcript was primarily expressed in tissues from the int ...	2015	25731714
silencing the odorant receptor co-receptor rproorco affects the physiology and behavior of the chagas disease vector rhodnius prolixus.	olfaction is one of the main sensory modalities that allow insects to interpret their environment. several proteins, including odorant-binding proteins (obps) and odorant receptors (ors), are involved in this process. odorant receptors are ion channels formed by a binding unit or and an odorant receptor co-receptor (orco). the main goal of this study was to characterize the orco gene of rhodnius prolixus (rproorco) and to infer its biological functions using gene silencing. the full-length rproo ...	2016	25747010
trypanosoma cruzi, etiological agent of chagas disease, is virulent to its triatomine vector rhodnius prolixus in a temperature-dependent manner.	it is often assumed that parasites are not virulent to their vectors. nevertheless, parasites commonly exploit their vectors (nutritionally for example) so these can be considered a form of host. trypanosoma cruzi, a protozoan found in mammals and triatomine bugs in the americas, is the etiological agent of chagas disease that affects man and domestic animals. while it has long been considered avirulent to its vectors, a few reports have indicated that it can affect triatomine fecundity. we test ...	2015	25793495
cardiac contractility structure-activity relationship and ligand-receptor interactions; the discovery of unique and novel molecular switches in myosuppressin signaling.	peptidergic signaling regulates cardiac contractility; thus, identifying molecular switches, ligand-receptor contacts, and antagonists aids in exploring the underlying mechanisms to influence health. myosuppressin (ms), a decapeptide, diminishes cardiac contractility and gut motility. myosuppressin binds to g protein-coupled receptor (gpcr) proteins. two drosophila melanogaster myosuppressin receptors (drmms-rs) exist; however, no mechanism underlying ms-r activation is reported. we predicted dr ...	2015	25793503
new scenarios of trypanosoma cruzi transmission in the orinoco region of colombia.	rhodnius prolixus, a blood-sucking triatomine with domiciliary anthropophilic habits, is the main vector of chagas disease. the current paradigm of trypanosoma cruzi transmission in columbia includes a sylvatic and domiciliary cycle co-existing with domestic and sylvatic populations of reservoirs. the aim of this study is to evaluate the population densities and relative abundance of triatomines and mammals that may be involved in the sylvatic cycle of chagas disease to clarify the epidemiologic ...	2015	25830543
female odours promote the activation of sheltered kissing bug rhodnius prolixus males and modulate their orientation.	the existence of a pheromone emitted during copulation has been reported for rhodnius prolixus (hemiptera: reduviidae) stål. adults possess one pair of metasternal glands (mgs) from which female r. prolixus release volatiles mainly at night. we investigated whether these volatiles emitted by adult r. prolixus can modulate sexual-related behaviours of opposite and/or same sex individuals. we first used a shelter bioassay to test if adult activity patterns can be affected by chemical signals emitt ...	2014	25855829
rhodnius prolixus interaction with trypanosoma rangeli: modulation of the immune system and microbiota population.	trypanosoma rangeli is a protozoan that infects a variety of mammalian hosts, including humans. its main insect vector is rhodnius prolixus and is found in several latin american countries. the r. prolixus vector competence depends on the t. rangeli strain and the molecular interactions, as well as the insect's immune responses in the gut and haemocoel. this work focuses on the modulation of the humoral immune responses of the midgut of r. prolixus infected with t. rangeli macias strain, conside ...	2015	25888720
effect of the physiognomy of attalea butyracea (arecoideae) on population density and age distribution of rhodnius prolixus (triatominae).	rhodnius prolixus stål, 1859 is one of the main vectors of trypanosoma (schyzotrypanum) cruzi chagas, 1909. in its natural forest environment, this triatomine is mainly found in palm tree crowns, where it easily establishes and develops dense populations. the aim of this study was to evaluate the effect of the physiognomy and reproductive status of attalea butyracea on the population relative density and age structure of r. prolixus and to determine the vector's population stratification accordi ...	2015	25889617
evaluation of reference genes for insect olfaction studies.	quantitative reverse transcription pcr (qrt-pcr) is a robust and accessible method to assay gene expression and to infer gene regulation. being a chain of procedures, this technique is subject to systematic error due to biological and technical limitations mainly set by the starting material and downstream procedures. thus, rigorous data normalization is critical to grant reliability and repeatability of gene expression quantification by qrt-pcr. a number of 'housekeeping genes', involved in bas ...	2015	25896676
allatotropin modulates myostimulatory and cardioacceleratory activities in rhodnius prolixus (stal).	haematophagous insects can ingest large quantities of blood in a single meal and eliminate high volumes of urine in the next few hours. this rise in diuresis is possible because the excretory activity of the malpighian tubules is facilitated by an increase in haemolymph circulation as a result of intensification of aorta contractions combined with an increase of the anterior midgut peristaltic waves. it has been previously described that haemolymph circulation during post-prandial diuresis is st ...	2015	25897783
toll signals regulate dorsal-ventral patterning and anterior-posterior placement of the embryo in the hemipteran rhodnius prolixus.	insect embryonic dorso-ventral patterning depends greatly on two pathways: the toll pathway and the bone morphogenetic protein pathway. while the relative contribution of each pathway has been investigated in holometabolous insects, their role has not been explored in insects with a hemimetabolous type of development. the hemimetabolous insect rhodnius prolixus, an important vector of chagas disease in the americas, develops from an intermediate germ band and displays complex movements during ka ...	2014	25908955
chitin is a component of the rhodnius prolixus midgut.	chitin is an essential component of the peritrophic matrix (pm), which is a structure that lines the insect's gut and protects against mechanical damage and pathogens. rhodnius prolixus (hemiptera: reduviidae) does not have a pm, but it has an analogous structure, the perimicrovillar membrane (pmm); chitin has not been described in this structure. here, we show that chitin is present in the r. prolixus midgut using several techniques. the ftir spectrum of the koh-resistant putative chitin-materi ...	2016	25910679
oviposition in the blood-sucking insect rhodnius prolixus is modulated by host odors.	triatomine bugs are blood-sucking insects, vectors of chagas disease. despite their importance, their oviposition behavior has received relatively little attention. some triatomines including rhodnius prolixus stick their eggs to a substrate. it is known that mechanical cues stimulate oviposition in this species. however, it is not clear if chemical signals play a role in this behavior. we studied the role of host cues, including host odor, in the oviposition behavior of the triatomine r. prolix ...	2015	25956818
genomic and functional characterization of a methoprene-tolerant gene in the kissing-bug rhodnius prolixus.	metamorphosis, which depends upon a fine balance between two groups of lipid-soluble hormones such as juvenile hormones (jhs) and ecdysteroids, is an important feature in insect evolution. while it is clear that the onset of metamorphosis depends on the decrease of jh levels, the way in which these hormones exert their activities is not fully understood in triatominae species. the discovery of a drosophila melanogaster mutant resistant to the treatment with the jh analog methoprene, led finally ...	2015	25963043
identification of g protein coupled receptors for opsines and neurohormones in rhodnius prolixus. genomic and transcriptomic analysis.	the importance of chagas disease motivated the scientific effort to obtain the complete genomic sequence of the vector species rhodnius prolixus, this information is also relevant to the understanding of triatomine biology in general. the central nervous system is the key regulator of insect physiology and behavior. neurohormones (neuropeptides and biogenic amines) are the chemical messengers involved in the regulation and integration of neuroendocrine signals. in insects, this signaling is main ...	2016	25976540
patterns of expression of odorant receptor genes in a chagas disease vector.	rhodnius prolixus is a triatomine bug acting as a relevant vector of chagas disease for which the genome sequence has been recently made available. based on this information, a set of olfactory (ors) and ionotropic receptor (irs) genes potentially related to olfactory processes was characterized, and the expression patterns along bug development and in different structures potentially involved in promoting chemosensory-mediated behaviors were studied. for this, diverse bioinformatic procedures w ...	2016	26003917
insecticide resistance in vector chagas disease: evolution, mechanisms and management.	chagas disease is a chronic parasitic infection restricted to america. the disease is caused by the protozoa trypanosoma cruzi, which is transmitted to human through the feces of infected triatomine insects. because no treatment is available for the chronic forms of the disease, vector chemical control represents the best way to reduce the incidence of the disease. chemical control has been based principally on spraying dwellings with insecticide formulations and led to the reduction of triatomi ...	2015	26003952
comparing de novo and reference-based transcriptome assembly strategies by applying them to the blood-sucking bug rhodnius prolixus.	high throughput sequencing capabilities have made the process of assembling a transcriptome easier, whether or not there is a reference genome. but the quality of a transcriptome assembly must be good enough to capture the most comprehensive catalog of transcripts and their variations, and to carry out further experiments on transcriptomics. there is currently no consensus on which of the many sequencing technologies and assembly tools are the most effective. many non-model organisms lack a refe ...	2016	26005117
identification, functional characterization, and pharmacological profile of a serotonin type-2b receptor in the medically important insect, rhodnius prolixus.	in the chagas disease vector, rhodnius prolixus, two diuretic hormones act synergistically to dramatically increase fluid secretion by the malpighian tubules (mts) during the rapid diuresis that is initiated upon engorgement of vertebrate blood. one of these diuretic hormones is the biogenic amine, serotonin (5-hydroxytryptamine, 5-ht), which controls a variety of additional activities including cuticle plasticization, salivary gland secretion, anterior midgut absorption, cardioacceleratory acti ...	2015	26041983
rhodnius prolixus life history outcomes differ when infected with different trypanosoma cruzi i strains.	the effect of a parasite on the life history of its vector is important for understanding and predicting disease transmission. chagas disease agent trypanosoma cruzi is a generalist parasite that is diverse across scales from its genetic diversity to the 100s of mammal and vector species it infects. its vertebrate hosts show quite variable responses to infection, however, to date there are no studies looking at how t. cruzi variability might result in variable outcomes in its invertebrate host. ...	2015	26078316
rhodnius prolixus supergene families of enzymes potentially associated with insecticide resistance.	chagas disease or american trypanosomiasis, is a potentially life-threatening illness caused by the protozoan parasite, trypanosoma cruzi. once known as an endemic health problem of poor rural populations in latin american countries, it has now spread worldwide. the parasite is transmitted by triatomine bugs, of which rhodnius prolixus (hemiptera, reduviidae, triatominae) is one of the vectors and a model organism. this species occurs mainly in central and south american countries where the dise ...	2016	26079630
calcium-induced lipid peroxidation is mediated by rhodnius heme-binding protein (rhbp) and prevented by vitellin.	lipid peroxidation is promoted by the quasi-lipoxygenase (ql) activity of heme proteins and enhanced by the presence of free calcium. unlike mammalian plasma, the hemolymph of rhodnius prolixus, a vector of chagas disease, contains both a free heme-binding protein (rhbp) and circulating lipoproteins. rhbp binds and prevents the heme groups of the proteins from participating in lipid peroxidation reactions. herein, we show that despite being bound to rhbp, heme groups promote lipid peroxidation t ...	2015	26111116
identification and characterization of the adipokinetic hormone/corazonin-related peptide signaling system in rhodnius prolixus.	the mammalian gonadotropin-releasing hormone is evolutionarily related to the arthropod adipokinetic hormone and the recently discovered adipokinetic hormone/corazonin-related peptide (acp). the function of the acp signaling system in arthropods is currently unknown. in the present study, we identify and characterize the acp signaling system in the kissing bug rhodnius prolixus. we isolated the complete cdna sequence encoding r. prolixus acp (rhopr-acp) and examined its expression pattern. rhopr ...	2015	26138617
adipokinetic hormone receptor gene identification and its role in triacylglycerol metabolism in the blood-sucking insect rhodnius prolixus.	adipokinetic hormone (akh) has been associated with the control of energy metabolism in a large number of arthropod species due to its role on the stimulation of lipid, carbohydrate and amino acid mobilization/release. in the insect rhodnius prolixus, a vector of chagas' disease, triacylglycerol (tag) stores must be mobilized to sustain the metabolic requirements during moments of exercise or starvation. besides the recent identification of the r. prolixus akh peptide, other components required ...	2016	26163435
comparative analysis of zygotic developmental genes in rhodnius prolixus genome shows conserved features on the tracheal developmental pathway.	most of the in-depth studies on insect developmental genetic have been carried out in the fruit fly drosophila melanogaster, an holometabolous insect, so much more still remains to be studied in hemimetabolous insects. having rhodnius prolixus sequenced genome available, we search for orthologue genes of zygotic signaling pathways, segmentation, and tracheogenesis in the r. prolixus genome and in three species of triatoma genus transcriptomes, concluding that there is a high level of gene conser ...	2015	26187251
detection of high percentage of trypanosoma cruzi infection, the etiologic agent of chagas disease, in wild populations of colombian caribbean triatomines.	in colombia it is estimated that about 900,000 persons are infected with t. cruzi. there are 25 triatomine species and 5 of them have been reported infected with t. cruzi in the colombian caribbean region. in order to obtain more information about the triatomine populations in this region, 89 wild triatomines were collected from four colombian departments. the most frequent specie collected was rhodnius pallescens (65%), followed by rhodnius prolixus (20%), panstrongylus geniculatus (10.1%) and ...	2015	26204001
rhodnius prolixus smells repellents: behavioural evidence and test of present and potential compounds inducing repellency in chagas disease vectors.	insect repellents are known since many decades ago and constitute a major tool for personal protection against the biting of mosquitoes. despite their wide use, the understanding of why and how repellents repel is relatively recent. in particular, the question about to what extent insects other than mosquitoes are repulsed by repellents remains open. we developed a series of bioassays aimed to test the performance of well established as well as potential repellent molecules on the chagas disease ...	2015	26210345
conserved molecular switch interactions in modeled cardioactive rf-nh2 peptide receptors: ligand binding and activation.	peptides may act through g protein-coupled receptors to influence cardiovascular performance; thus, delineating mechanisms involved in signaling is a molecular-based strategy to influence health. molecular switches, often represented by conserved motifs, maintain a receptor in an inactive state. however, once the switch is broken, the transmembrane regions move and activation occurs. the molecular switches of drosophila melanogaster myosuppressin (ms) receptors were previously identified to incl ...	2015	26211890
thermosensation and the trpv channel in rhodnius prolixus.	the thermal sense of triatomine bugs, vectors of chagas disease, is unique among insects. not only do these bugs exhibit the highest sensitivity to heat known in any animal up to date, but they can also perceive the infrared radiation emitted by the body of their warm-blooded hosts. the sensory basis of this capacity has just started to be unravelled. to shed additional light on our understanding of thermosensation, we initiated an analysis of the genetic basis of the thermal sense in rhodnius p ...	2015	26225467
transcription factors, chromatin proteins and the diversification of hemiptera.	availability of complete genomes provides a means to explore the evolution of enormous developmental, morphological, and behavioral diversity among insects. hemipterans in particular show great diversity of both morphology and life history within a single order. to better understand the role of transcription regulators in the diversification of hemipterans, using sequence profile searches and hidden markov models we computationally analyzed transcription factors (tfs) and chromatin proteins (cps ...	2016	26226651
the impact of landscape transformation on the reinfestation rates of rhodnius prolixus in the orinoco region, colombia.	trypanosoma cruzi, the agent responsible for chagas disease, is widely distributed in the americas in association to insect vectors and animal reservoirs. triatomines are common vectors of chagas disease and they vary in their distribution and affinity to human or sylvatic environments. thus, control programs that include house spraying have been successful in regions were insects have a tendency to domiciliation. in the orinoco region of colombia, rhodnius prolixus is the main vector presenting ...	2015	26254003
circadian modulation of learning ability in a disease vector insect, rhodnius prolixus.	despite the drastic consequences it may have on the transmission of parasites, the ability of disease vectors to learn and retain information has just begun to be characterised. the kissing bug rhodnius prolixus, a vector of chagas disease, is an excellent model, particularly because conditioning the proboscis extension response (per) constitutes a valuable paradigm to study their cognitive abilities under carefully controlled conditions. another characteristic of these bugs is the temporal orga ...	2015	26276862
trypanosomes modify the behavior of their insect hosts: effects on locomotion and on the expression of a related gene.	as a result of evolution, the biology of triatomines must have been significantly adapted to accommodate trypanosome infection in a complex network of vector-vertebrate-parasite interactions. arthropod-borne parasites have probably developed mechanisms, largely still unknown, to exploit the vector-vertebrate host interactions to ensure their transmission to suitable hosts. triatomines exhibit a strong negative phototaxis and nocturnal activity, believed to be important for insect survival agains ...	2015	26291723
protein 2de reference map of the anterior midgut of the blood-sucking bug rhodnius prolixus.	rhodnius prolixus is an important vector of trypanosoma cruzi, the causative agent of chagas' disease, an illness that affects 20% of latin america population. the obligatory course of the parasite in the vector digestive tract has made it an important target for investigation in order to control the parasite transmission and thus interrupt its biological cycle in the insect vector. therefore, an insight into the vector midgut physiology is valuable for insect control as well as to provide poten ...	2015	26314381
vital statistics of triatominae (hemiptera: reduviidae) under laboratory conditions: iv. panstrongylus geniculatus.	a cohort of 100 eggs of panstrongylus geniculatus (latreille) was reared in the laboratory under constant conditions (temperature 26 ± 1°c, 60 ± 10% rh), with mortality and fecundity data recorded weekly. we calculated stage-specific development times, age-specific mortality and fecundity (18.4 eggs/♀/wk), and stage-specific and total preadult mortality (31.6%), and the weekly intrinsic rate of natural increase (r(o) = 0.096), the finite population growth rate (λ = 1.109), the net reproductive r ...	2015	26336251
global climate change effects on venezuela's vulnerability to chagas disease is linked to the geographic distribution of five triatomine species.	we analyzed the possible effects of global climate change on the potential geographic distribution in venezuela of five species of triatomines (eratyrus mucronatus (stal, 1859), panstrongylus geniculatus (latreille, 1811), rhodnius prolixus (stål, 1859), rhodnius robustus (larrousse, 1927), and triatoma maculata (erichson, 1848)), vectors of trypanosoma cruzi, the etiological agent of chagas disease. to obtain the future potential geographic distributions, expressed as climatic niche suitability ...	2015	26336258
transposition burst of mariner-like elements in the sequenced genome of rhodnius prolixus.	transposable elements (tes) are widespread in insect's genomes. however, there are wide differences in the proportion of the total dna content occupied by these repetitive sequences in different species. we have analyzed the tes present in r. prolixus (vector of the chagas disease) and showed that 3.0% of this genome is occupied by class ii tes, belonging mainly to the tc1-mariner superfamily (1.65%) and mites (1.84%). interestingly, most of this genomic content is due to the expansion of two su ...	2016	26363296
identification of a selenium-dependent glutathione peroxidase in the blood-sucking insect rhodnius prolixus.	the selenium-dependent glutathione peroxidase (segpx) is a well-studied enzyme that detoxifies organic and hydrogen peroxides and provides cells or extracellular fluids with a key antioxidant function. the presence of a segpx has not been unequivocally demonstrated in insects. in the present work, we identified the gene and studied the function of a rhodnius prolixus segpx (rpsegpx). the rpsegpx mrna presents the uga codon that encodes the active site selenocysteine (sec) and a corresponding sec ...	2016	26392061
eco-epidemiological study of an endemic chagas disease region in northern colombia reveals the importance of triatoma maculata (hemiptera: reduviidae), dogs and didelphis marsupialis in trypanosoma cruzi maintenance.	in colombia, rhodnius prolixus and triatoma dimidiata are the main domestic triatomine species known to transmit t. cruzi. however, there are multiple reports of t. cruzi transmission involving secondary vectors. in this work, we carried out an eco-epidemiological study on margarita island, located in the caribbean region of colombia, where chagas disease is associated with non-domiciliated vectors.	2015	26394766
exploring the role of rhodtestolin, a cardio-inhibitor from the testes of rhodnius prolixus, in relation to the structure and function of reproductive organs in insect vectors of chagas disease.	rhodtestolin is a cardio-inhibitor that was first discovered in testes extracts of the blood-feeding insect, rhodnius prolixus. its role in reproduction remains unconfirmed, but if delivered to the female during spermatophore formation, it may serve to calm the female and/or relax the vaginal muscles to facilitate delivery and storage of the spermatophore. we describe here the anatomy of reproductive organs in r. prolixus and show that rhodtestolin is present in a low-molecular weight fraction o ...	2013	26462525
monitoring of the parasite load in the digestive tract of rhodnius prolixus by combined qpcr analysis and imaging techniques provides new insights into the trypanosome life cycle.	here we report the monitoring of the digestive tract colonization of rhodnius prolixus by trypanosoma cruzi using an accurate determination of the parasite load by qpcr coupled with fluorescence and bioluminescence imaging (bli). these complementary methods revealed critical steps necessary for the parasite population to colonize the insect gut and establish vector infection.	2015	26496442
[entomological study of trypanosoma cruzi vectors in the rural communities of sucre state, venezuela].	the ecological niche of reduvidae vectors has been modified due to environmental changes and human encroachment into the rural areas.	2015	26535547
risks associated with dispersive nocturnal flights of sylvatic triatominae to artificial lights in a model house in the northeastern plains of colombia.	control initiatives and continuous surveillance of vector-borne transmission have proved to be effective measures for diminishing the incidence of chagas disease in endemic countries. however, the active dispersal of infected sylvatic adult triatomines by flight represents one of the main obstacles to eliminating domestic transmission.	2015	26582012
genome of rhodnius prolixus, an insect vector of chagas disease, reveals unique adaptations to hematophagy and parasite infection.	rhodnius prolixus not only has served as a model organism for the study of insect physiology, but also is a major vector of chagas disease, an illness that affects approximately seven million people worldwide. we sequenced the genome of r. prolixus, generated assembled sequences covering 95% of the genome (∼ 702 mb), including 15,456 putative protein-coding genes, and completed comprehensive genomic analyses of this obligate blood-feeding insect. although immune-deficiency (imd)-mediated immune ...	2015	26627243
behavioral and toxicological responses of rhodnius prolixus (hemiptera: reduviidae) to the insect repellents deet and ir3535.	,n-diethyl-3-methylbenzamide (deet) is a broad-spectrum insect repellent used by millions of people since the 1950s. ethyl 3-[acetyl(butyl)amino]propanoate (ir3535) is a repellent developed more recently that is still not used as extensively. this study compares the behavioral and toxicological effects of both substances in fifth-instar nymphs of the blood-sucking bug rhodnius prolixus stål (hemiptera: reduviidae), one of the main vectors of american trypanosomiasis (chagas disease). repellency ...	2016	26637386
mariner transposons are sailing in the genome of the blood-sucking bug rhodnius prolixus.	the triatomine bug rhodnius prolixus is a vector of trypanosoma cruzi, which causes the chagas disease in latin america. r. prolixus can also transfer transposable elements horizontally across a wide range of species. we have taken advantage of the availability of the 700 mbp complete genome sequence of r. prolixus to study the dynamics of invasion and persistence of transposable elements in this species.	2015	26666222
human trypanosomiasis in the eastern plains of colombia: new transmission scenario.	characteristics of trypanosoma cruzi infection were studied in a rural area of the eastern plains of colombia. using enzyme-linked immunosorbent assay and indirect fluorescent-antibody tests, the infection was determined in 11.6% of the inhabitants of 142 dwellings. during 6 months of community surveillance, in 42.3% dwellings, 609 triatomines were collected (597 rhodnius prolixus and seven, three, one, and one of panstrongylus geniculatus, psammolestes arturi, eratyrus mucronatus, and triatoma ...	2016	26728765
the role of light in chagas disease infection risk in colombia.	chagas disease is the most important vector-borne disease in latin america and rhodnius prolixus is the main vector in colombia. control strategies in this region have shown poor outcomes due to the insect's ability to disperse between the sylvatic and the domestic habitat. because insect migration to houses is responsible to sustain contact rates between vectors and humans, understanding the risk factors that promote migration could be important in designing control strategies. in this respect, ...	2016	26732186
identification of the first insulin-like peptide in the disease vector rhodnius prolixus: involvement in metabolic homeostasis of lipids and carbohydrates.	insulin-like peptides (ilps) are functional analogs of insulin and have been identified in many insect species. the insulin cell signaling pathway is a conserved regulator of metabolism, and in insects, as well as in other animals, can modulate physiological functions associated with the metabolism of lipids and carbohydrates. in the present study, we have identified the first ilp from the rhodnius prolixus genome (termed rhopr-ilp) and investigated its involvement in energy metabolism of unfed ...	2016	26742603
genotyping of trypanosoma cruzi dtus and trypanosoma rangeli genetic groups in experimentally infected rhodnius prolixus by pcr-rflp.	the specific detection and genetic typing of trypanosomes that infect humans, mammalian reservoirs, and vectors is crucial for diagnosis and epidemiology. we utilized a pcr-rflp assay that targeted subunit ii of cytochrome oxidase and 24sα-rdna to simultaneously detect and discriminate six trypanosoma cruzi discrete typing units (dtus) and two genetic groups of trypanosoma rangeli (kp1+/kp1-) in intestinal contents of experimentally infected rhodnius prolixus. the pcr assays showed that in 23 of ...	2016	26792202
biostable insect kinin analogs reduce blood meal and disrupt ecdysis in the blood-gorging chagas' disease vector, rhodnius prolixus.	rhodnius prolixus is a blood-gorging hemipteran that takes blood meals that are approximately 10 times its body weight. this blood meal is crucial for growth and development and is needed to ensure a successful molt into the next instar. kinins are a multifunctional family of neuropeptides which have been shown to play a role in the control of feeding in a variety of insects. in this study, two biostable aib-containing kinin analogs were tested to see if they interfere with blood-feeding and sub ...	2016	26807486
colonization of rhodnius prolixus gut by trypanosoma cruzi involves an extensive parasite killing.	trypanosoma cruzi, the etiological agent of chagas disease, is ingested by triatomines during their bloodmeal on an infected mammal. aiming to investigate the development and differentiation of t. cruzi inside the intestinal tract of rhodnius prolixus at the beginning of infection we fed insects with cultured epimastigotes and blood trypomastigotes from infected mice to determine the amount of recovered parasites after ingestion. approximately 20% of the ingested parasites was found in the insec ...	2016	26818093
first report of y-linked genes in the kissing bug rhodnius prolixus.	due to an abundance of repetitive dna, the annotation of heterochromatic regions of the genome such as the y chromosome is problematic. the y chromosome is involved in key biological functions such as male-fertility and sex-determination and hence, accurate identification of its sequences is vital. the hemipteran insect rhodnius prolixus is an important vector of chagas disease, a trypanosomiasis affecting 6-7 million people worldwide. here we report the identification of the first y-linked gene ...	2016	26861771
an insight into the triabin protein family of american hematophagous reduviids: functional, structural and phylogenetic analysis.	a transcriptomic analysis of the saliva of t. pallidipennis together with a short proteomic analysis were carried out to reveal novel primary structures of the lipocalin/triabin protein families in this reduviid. although triabins share some structural characteristics to lipocalins and they are classified as in the calcyn/lipocalin superfamily, triabins differ from lipocalins in the direction of β-strands in the general conformation of the β-barrel. the triabin protein family encompasses a wide ...	2016	26891325
symbiont-mediated rna interference in insects.	rna interference (rnai) methods for insects are often limited by problems with double-stranded (ds) rna delivery, which restricts reverse genetics studies and the development of rnai-based biocides. we therefore delegated to insect symbiotic bacteria the task of: (i) constitutive dsrna synthesis and (ii) trauma-free delivery. rnaseiii-deficient, dsrna-expressing bacterial strains were created from the symbionts of two very diverse pest species: a long-lived blood-sucking bug, rhodnius prolixus, ...	2016	26911963
here, there, and everywhere: the ubiquitous distribution of the immunosignaling molecule lysophosphatidylcholine and its role on chagas disease.	chagas disease is a severe illness, which can lead to death if the patients are not promptly treated. the disease is caused by the protozoan parasite trypanosoma cruzi, which is mostly transmitted by a triatomine insect vector. there are 8-10 million people infected with t. cruzi in the world, but the transmission of such disease by bugs occurs only in the americas, especially latin america. chronically infected patients will develop cardiac diseases (30%) and up digestive, neurological, or mixe ...	2016	26925065
everybody loves sugar: first report of plant feeding in triatomines.	triatomines, which are the vectors of trypanosoma cruzi, have been considered to be exclusive blood feeders for more than 100 years, since the discovery of chagas disease.	2016	26928036
correction for mesquita et al., genome of rhodnius prolixus, an insect vector of chagas disease, reveals unique adaptations to hematophagy and parasite infection.		2016	26929356
impact of trypanosoma cruzi on antimicrobial peptide gene expression and activity in the fat body and midgut of rhodnius prolixus.	rhodnius prolixus is a major vector of trypanosoma cruzi, the causative agent of chagas disease in latin america. in natural habitats, these insects are in contact with a variety of bacteria, fungi, virus and parasites that they acquire from both their environments and the blood of their hosts. microorganism ingestion may trigger the synthesis of humoral immune factors, including antimicrobial peptides (amps). the objective of this study was to compare the expression levels of amps (defensins an ...	2016	26931761
comparative proteomic analysis of the saliva of the rhodnius prolixus, triatoma lecticularia and panstrongylus herreri triatomines reveals a high interespecific functional biodiversity.	triatomines are hematophagous arthropods that transmit trypanosoma cruzi and trypanosoma rangeli. feeding behavior and pathogen transmission is known to vary between the different species, and this characteristic is directly or indirectly dependent on the bioactive molecules of the saliva that facilitate the vector-host-parasite interaction. here, we identify, characterize and compare the sialoproteomic (from the greek sialo: saliva) repertoire of important species of the main triatomine genera ...	2016	26940473
amino acids trigger down-regulation of superoxide via torc pathway in the midgut of rhodnius prolixus.	sensing incoming nutrients is an important and critical event for intestinal cells to sustain life of the whole organism. the torc is a major protein complex involved in monitoring the nutritional status and is activated by elevated amino acid concentrations. an important feature of haematophagy is that huge amounts of blood are ingested in a single meal, which results in the release of large quantities of amino acids, together with the haemoglobin prosthetic group, haem, which decomposes hydrop ...	2016	26945025
the acbp gene family in rhodnius prolixus: expression, characterization and function of rpacbp-1.	the acyl-coa-binding proteins (acbp) constitute a family of conserved proteins that bind acyl-coa with high affinity and protect it from hydrolysis. thus, acbps may have essential roles in basal cellular lipid metabolism. the genome of the insect rhodnius prolixus encodes five acbp genes similar to those described for other insect species. the qpcr analysis revealed that these genes have characteristic expression profiles in insect organs, suggesting that they have specific roles in insect physi ...	2016	27001070
haematophagy is costly: respiratory patterns and metabolism during feeding in rhodnius prolixus.	feeding on the blood of vertebrates is a risky task for haematophagous insects and it can be reasonably assumed that it should also be costly in terms of energetic expenditure. blood circulates inside vessels and it must be pumped through narrow tubular stylets to be ingested. we analysed the respiratory pattern and the energetic cost of taking a blood meal in rhodnius prolixus using flow-through and stop-flow respirometry to measure carbon dioxide emission, oxygen consumption and water loss bef ...	2016	27045096
development of data for the identification and characterization of proteins found in rhodnius prolixus, triatoma lecticularia and panstrongylus herreri.	the data presented here were obtained from the saliva of three triatominae, rhodnius prolixus, triatoma lecticularia and panstrongylus herreri from montandon et al. study, doi:10.1016/j.ibmb.2016.02.009 [3]. these data were obtained from spectra generated by the mass spectrometry of proteins observed through the analysis of 2-d electrophoretic profiles. the data were analyzed according to the uniprot code, protein name, protein group, isoelectric point and molecular weight, electrophoretic profi ...	2016	27077085
nitrobindin: an ubiquitous family of all β-barrel heme-proteins.	rhodnius prolixus nitrophorins (rp-nps), arabidopsis thaliana nitrobindin (at-nb), and homo sapiens thap4 (hs-thap4) are the unique known proteins that use a β-barrel fold to bind ferric heme, which is devoted to no transport and/or catalysis. the eight-stranded antiparallel β-barrel rp-nps, which represent the only heme-binding lipocalins, are devoted to deliver no into the blood vessel of the host and to scavenge histamine during blood sucking. regarding nbs, crystallographic data suggest the ...	2016	27080126
long-chain acyl-coa synthetase 2 knockdown leads to decreased fatty acid oxidation in fat body and reduced reproductive capacity in the insect rhodnius prolixus.	long-chain acyl-coa esters are important intermediates in lipid metabolism and are synthesized from fatty acids by long-chain acyl-coa synthetases (acsl). the hematophagous insect rhodnius prolixus, a vector of chagas' disease, produces glycerolipids in the midgut after a blood meal, which are stored as triacylglycerol in the fat body and eggs. we identified twenty acyl-coa synthetase genes in r. prolixus, two encoding acsl isoforms (rhopracsl1 and rhopracsl2). rhopracsl1 transcripts increased i ...	2016	27091636
rhodnius prolixus intoxicated.	rhodnius prolixus (hemiptera: reduviidae) is a hematophagous insect native from south america. by the end of the 20th century, it was one of the main vectors of chagas disease in venezuela, colombia, several central american countries and southern mexico. the aim of the present article is to review the literature regarding r. prolixus toxicology. british entomologist vincent b. wigglesworth carried out the first studies on this subject over seventy years ago. a wide bibliographical search allowe ...	2017	27113321
rhodnius prolixus and r. robustus (hemiptera: reduviidae) nymphs show different locomotor patterns on an automated recording system.	circadian rhythms of triatomines, vectors of the etiological agent trypanosoma cruzi responsible for chagas disease, have been extensively studied in adults of the two most epidemiologically relevant vector species, rhodnius prolixus and triatoma infestans. however, little attention has been dedicated to the activity patterns in earlier developmental stages, even though triatomine nymphs are equally capable of transmitting t. cruzi to humans. because circadian rhythms may differ even between clo ...	2016	27121502
structure and ligand-binding mechanism of a cysteinyl leukotriene-binding protein from a blood-feeding disease vector.	blood-feeding disease vectors mitigate the negative effects of hemostasis and inflammation through the binding of small-molecule agonists of these processes by salivary proteins. in this study, a lipocalin protein family member (ltbp1) from the saliva of rhodnius prolixus, a vector of the pathogen trypanosoma cruzi, is shown to sequester cysteinyl leukotrienes during feeding to inhibit immediate inflammatory responses. calorimetric binding experiments showed that ltbp1 binds leukotrienes c4 (ltc ...	2016	27124118
trypanosoma cruzi-trypanosoma rangeli co-infection ameliorates negative effects of single trypanosome infections in experimentally infected rhodnius prolixus.	trypanosoma cruzi, causative agent of chagas disease, co-infects its triatomine vector with its sister species trypanosoma rangeli, which shares 60% of its antigens with t. cruzi. additionally, t. rangeli has been observed to be pathogenic in some of its vector species. although t. cruzi-t. rangeli co-infections are common, their effect on the vector has rarely been investigated. therefore, we measured the fitness (survival and reproduction) of triatomine species rhodnius prolixus infected with ...	2016	27174360
allatostatin-c antagonizes the synergistic myostimulatory effect of allatotropin and serotonin in rhodnius prolixus (stal).	haematophagous insects can ingest large quantities of blood in a single meal producing a large quantity of urine in the following hours to eliminate the excess of water and mineral ions incorporated. the excretory activity of the malpighian tubules is facilitated by an increase in haemolymph circulation as a result of the intensification of aorta contractions, combined with an increase of anterior midgut peristaltic waves. we have recently shown that haemolymph circulation during post-prandial d ...	2016	27174746
ecdysis period of rhodnius prolixus head investigated using phase contrast synchrotron microtomography.	microtomography using synchrotron sources is a useful tool in biological imaging research since the phase coherence of synchrotron beams can be exploited to obtain images with high contrast resolution. this work is part of a series of works using phase contrast synchrotron microtomography in the study of rhodnius prolixus head, the insect vector of chagas' disease, responsible for about 12,000 deaths per year. the control of insect vector is the most efficient method to prevent this disease and ...	2016	27184550
cloning and expression of long neuropeptide f and the role of fmrfamide-like peptides in regulating egg production in the chagas vector, rhodnius prolixus.	long neuropeptide f (npf) is a neuropeptide implicated in the control of feeding, digestion and reproduction in various insect species. here we have isolated the cdna sequence encoding npf in rhodnius prolixus (rhoprnpf). the rhoprnpf gene is composed of 3 exons and 2 introns, one of which is present in the peptide coding region. rhoprnpf is 42 amino acids long and has the characteristic rfamide c-terminus, which is common of fmrfamide-like peptides (flps). quantitative pcr (qpcr) shows that rho ...	2016	27189503
experimental infection of rhodnius prolixus (hemiptera, triatominae) with mycobacterium leprae indicates potential for leprosy transmission.	leprosy is a chronic dermato-neurological disease caused by infection with mycobacterium leprae. in 2013 almost 200,000 new cases of leprosy were detected around the world. since the first symptoms take from years to decades to appear, the total number of asymptomatic patients is impossible to predict. although leprosy is one of the oldest records of human disease, the mechanisms involved with its transmission and epidemiology are still not completely understood. in the present work, we experime ...	2016	27203082
neuropeptides in the regulation of rhodnius prolixus physiology.	in the kissing bug rhodnius prolixus, events such as diuresis, antidiuresis, development and reproduction are triggered by blood feeding. hence, these events can be accurately timed, facilitating physiological experiments. this, combined with its relatively big size, makes r. prolixus an excellent model in insect neuroendocrinological studies. the importance of r. prolixus as a chagas' disease vector as much as an insect model has motivated the sequencing of its genome in recent years, facilitat ...	2017	27210592
what is the 'true' effect of trypanosoma rangeli on its triatomine bug vector?	the phrase, "t. rangeli is pathogenic to its insect vector," is commonly found in peer-reviewed publications on the matter, such that it has become the orthodox view of this interaction. in a literature survey, we identified over 20 papers with almost the exact phrase and several others alluding to it. the idea is of particular importance in triatomine population dynamics and the study of vector-borne t. cruzi transmission, as it could mean that triatomines infected with t. rangeli have lower fi ...	2016	27232121
isolation and characterization of the corticotropin-releasing factor-related diuretic hormone receptor in rhodnius prolixus.	rhodnius prolixus, the vector of human chagas disease, is a hemipteran insect that undergoes rapid post-feeding diuresis following ingestion of a blood meal that can be up to 10 times its initial body weight. corticotropin-releasing factor-related diuretic hormone (rhopr-crf/dh) and serotonin are neurohormones that are synergistic in increasing rates of fluid secretion by malpighian tubules during this rapid post-feeding diuresis. a rhopr-crf/dh receptor transcript has now been isolated and char ...	2016	27237375
reproductive physiology in the blood feeding insect, rhodnius prolixus, from copulation to the control of egg production.	the study of copulation and the control of egg production in the blood-feeding insect, rhodnius prolixus, continues to offer a fertile ground for the cultivation of new insights into the physiological processes associated with reproduction. we begin this paper by describing the male organs of copulation showing that the aedeagus contains a structure which may serve as part of a pumping mechanism or valve to enable the male to fully pack the vagina with his secretions. we then summarize previousl ...	2017	27286950
circadian control of prothoracicotropic hormone release in an adult insect and the induction of its rhythmicity by light cues.	the insect neuropeptide prothoracicotropic hormone (ptth) is a critical regulator of larval development. we recently demonstrated that ptth is also present in adult rhodnius prolixus and is released by adult brains in vitro with a clear daily rhythm during egg development. here, we employ a well-established in vitro bioassay, to show that the daily rhythm of ptth release by brains in vitro is under circadian control since it persists in aperiodic conditions with a free running period of around 2 ...	2016	27371111
triatomine physiology in the context of trypanosome infection.	triatomines are hematophagous insects that feed on the blood of vertebrates from different taxa, but can occasionally also take fluids from invertebrate hosts, including other insects. during the blood ingestion process, these insects can acquire diverse parasites that can later be transmitted to susceptible vertebrates if they complete their development inside bugs. trypanosoma cruzi, the etiological agent of chagas disease, and trypanosoma rangeli are protozoan parasites transmitted by triatom ...	2017	27401496
humoral and cellular immune responses induced by the urease-derived peptide jaburetox in the model organism rhodnius prolixus.	although the entomotoxicity of plant ureases has been reported almost 20 years ago, their insecticidal mechanism of action is still not well understood. jaburetox is a recombinant peptide derived from one of the isoforms of canavalia ensiformis (jack bean) urease that presents biotechnological interest since it is toxic to insects of different orders. previous studies of our group using the chagas disease vector and model insect rhodnius prolixus showed that the treatment with jack bean urease ( ...	2016	27455853
temperature and parasite life-history are important modulators of the outcome of trypanosoma rangeli-rhodnius prolixus interactions.	trypanosoma rangeli is a protozoan parasite, which does not cause disease in humans, although it can produce different levels of pathogenicity to triatomines, their invertebrate hosts. we tested whether infection imposed a temperature-dependent cost on triatomine fitness using t. rangeli with different life histories. parasites cultured only in liver infusion tryptose medium (cultured) and parasites exposed to cyclical passages through mice and triatomines (passaged) were used. we held infected ...	2016	27460893
tyrosine detoxification is an essential trait in the life history of blood-feeding arthropods.	blood-feeding arthropods are vectors of infectious diseases such as dengue, zika, chagas disease, and malaria [1], and vector control is essential to limiting disease spread. because these arthropods ingest very large amounts of blood, a protein-rich meal, huge amounts of amino acids are produced during digestion. previous work on rhodnius prolixus, a vector of chagas disease, showed that, among all amino acids, only tyrosine degradation enzymes were overexpressed in the midgut compared to other ...	2016	27476595
functional evaluation of heat shock proteins 70 (hsp70/hsc70) on rhodnius prolixus (hemiptera, reduviidae) physiological responses associated with feeding and starvation.	blood-sucking vectors must overcome thermal stress caused by intake of proportionally large amounts of warm blood from their hosts. in response to this, heat shock proteins (hsps) such as the widely studied hsp70 family (the inducible hsp70 and the cognate form hsc70, known for their role in preserving essential cellular functions) are rapidly up-regulated in their tissues. the triatomine rhodnius prolixus is an important vector of trypanosoma cruzi, the causative pathogen of chagas' disease, an ...	2016	27491440
mitochondria and the insect steroid hormone receptor (ecr): a complex relationship.	the actions of the insect steroid molting hormones, ecdysteroids, on the genome of target cells has been well studied, but little is known of their extranuclear actions. we previously showed in rhodnius prolixus that much of the ecdysteroid receptor (ecr) resides in the cytoplasm of various cell types and undergoes shuttling between nucleus and cytoplasm with circadian periodicity, possibly using microtubules as tracks for translocation to the nucleus. here we report that cytoplasmic ecr appears ...	2016	27497706
metabolism and gas exchange patterns in rhodnius prolixus.	insect's metabolic rate and patterns of gas-exchange varies according to different factors such as: species, activity, mass, and temperature among others. one particular striking pattern of gas-exchange in insects is discontinuous gas-exchange cycles, for which many different hypotheses regarding their evolution have been stated. this article does not pretend to be an extensive review on the subject, rather to focus on the work performed on the haematophagous bug rhodnius prolixus, a model organ ...	2017	27498143
identification and characterization of the corazonin receptor and possible physiological roles of the corazonin-signaling pathway in rhodnius prolixus.	neuropeptides control many physiological and endocrinological processes in animals, acting as neuroactive chemicals within the central and peripheral nervous systems. corazonin (crz) is one such neuropeptide that has a variety of physiological roles associated with control of heartbeat, ecdysis behavior initiation, and cuticle coloration. these physiological effects are mediated by the crz receptor (crzr). in order to understand the role of the crz-signaling pathway in rhodnius prolixus, the cdn ...	2016	27536213
glycogen synthase kinase-3 is involved in glycogen metabolism control and embryogenesis of rhodnius prolixus.	rhodnius prolixus is a blood-feeding insect that transmits trypanosoma cruzi and trypanosoma rangeli to vertebrate hosts. rhodnius prolixus is also a classical model in insect physiology, and the recent availability of r. prolixus genome has opened new avenues on triatomine research. glycogen synthase kinase 3 (gsk-3) is classically described as a key enzyme involved in glycogen metabolism, also acting as a downstream component of the wnt pathway during embryogenesis. gsk-3 has been shown to be ...	2016	27574112
behavioral fever response in rhodnius prolixus (reduviidae: triatominae) to intracoelomic inoculation of trypanosoma cruzi.	behavioral fever is a response to infections with microorganisms observed in some poikilothermic animals. rhodnius prolixus is involved in the transmission of two parasites: trypanosoma cruzi (pathogenic for humans and transmitted in feces) and trypanosoma rangeli (non-pathogenic for humans, pathogenic for rhodnius and transmitted by the bite of an infected individual). only t. rangeli is found in the hemolymph of rhodnius as it travels to the salivary glands.	2017	27598628
lipid metabolism in rhodnius prolixus: lessons from the genome.	the kissing bug rhodnius prolixus is both an important vector of chagas' disease and an interesting model for investigation into the field of physiology, including lipid metabolism. the publication of this insect genome will bring a huge amount of new molecular biology data to be used in future experiments. although this work represents a promising scenario, a preliminary analysis of the sequence data is necessary to identify and annotate the genes involved in lipid metabolism. here, we used bio ...	2017	27697616
[strategy for determining a baseline in areas of vector interruption for chagas disease].	objectives present a strategy to determine the baseline in endemic areas in the process of vector interruption for chagas disease (chd). methods a social and environmental questionnaire and an entomological survey evaluated the physical conditions of dwellings, the inhabitants' knowledge of chd, the entomological triatomine indicators and the statistical relationship among these variables. results colonization and natural infection with trypanosoma cruzi exist in rhodnius prolixus, the principal ...	2016	27706434
under-expression of chemosensory genes in domiciliary bugs of the chagas disease vector triatoma brasiliensis.	in latin america, the bloodsucking bugs triatominae are vectors of trypanosoma cruzi, the parasite that causes chagas disease. chemical elimination programs have been launched to control chagas disease vectors. however, the disease persists because native vectors from sylvatic habitats are able to (re)colonize houses-a process called domiciliation. triatoma brasiliensis is one example. because the chemosensory system allows insects to interact with their environment and plays a key role in insec ...	2016	27792774
triflumuron effects on the physiology and reproduction of rhodnius prolixus adult females.	we evaluated the efficacy of the growth regulator triflumuron (tfm) in inducing mortality and disrupting both oviposition and egg hatching in rhodnius prolixus adult females. tfm was administered via feeding, topically or by continuous contact with impregnated surfaces. feeding resulted in mild biological effects compared with topical and impregnated surfaces. one day after treatment, the highest mortality levels were observed with topical surface and 30 days later both topical and impregnated s ...	2016	27822479
the presence of the ancestral insect telomeric motif in kissing bugs (triatominae) rules out the hypothesis of its loss in evolutionarily advanced heteroptera (cimicomorpha).	next-generation sequencing data analysis on triatoma infestans klug, 1834 (heteroptera, cimicomorpha, reduviidae) revealed the presence of the ancestral insect (ttagg)n telomeric motif in its genome. fluorescence in situ hybridization confirms that chromosomes bear this telomeric sequence in their chromosomal ends. furthermore, motif amount estimation was about 0.03% of the total genome, so that the average telomere length in each chromosomal end is almost 18 kb long. we also detected the presen ...	2016	27830050
description of rhodnius marabaensis sp. n. (hemiptera, reduviidae, triatominae) from pará state, brazil.	rhodnius marabaensissp. n. was collected on 12 may 2014 in the murumurú environmental reserve in the city of marabá, pará state, brazil. this study was based on previous consultation of morphological descriptions of 19 rhodnius species and compared to the identification key for the genus rhodnius. the examination included specimens from 18 rhodnius species held in the brazilian national and international triatomine taxonomy reference laboratory in the oswaldo cruz institute in rio de janeiro, br ...	2016	27833419