Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| molecular cloning of snyder-theilen feline leukemia and sarcoma viruses: comparative studies of feline sarcoma virus with its natural helper virus and with moloney murine sarcoma virus. | extrachromosomal dna obtained from mink cells acutely infected with the snyder-theilen (st) strain of feline sarcoma virus (feline leukemia virus) [fesv(felv)] was fractionated electrophoretically, and samples enriched for felv and fesv linear intermediates were digested with ecori and cloned in lambda phage. hybrid phages were isolated containing either fesv or felv dna "inserts" and were characterized by restriction enzyme analysis, r-looping with purified 26 to 32s viral rna, and heteroduplex ... | 1980 | 6246254 |
| in vitro production of immunosuppressive factors by murine sarcoma virus-transformed mouse fibroblasts. | murine sarcoma virus-transformed mouse fibroblasts produce potent immunosuppressive factors (isf) in vitro. the partially purified isf inhibited thymocyte proliferation induced by concanavalin a or phytohemagglutinin plus lymphocyte activating factor (interleukin 1), lipopolysaccharide-induced spleen cell proliferation, the in vitro splenic anti-sheep erythrocyte plaque-forming cell response, and the generation of alloantigen-specific cytotoxic t cells. the effect of isf on thymocyte proliferati ... | 1980 | 6246526 |
| localization of the src gene product of the harvey strain of msv to plasma membrane of transformed cells by electron microscopic immunocytochemistry. | using electron microscopic immunocytochemistry, we have investigated the intracellular location of the src protein (p21) in cells transformed by the harvey strain of murine sarcoma virus (ha-msv). antibodies to p21 were derived from tumor-bearing rats inoculated with ha-nrk cells. the distribution of p21 in intracellular sites in mdck dog cells transformed by ha-msv was examined and quantified using a recently developed immunocytochemical technique. more than 95% of p21 was localized to the inne ... | 1980 | 6247068 |
| double transformation of indian muntjac cells by avian and murine sarcoma viruses. | avian sarcoma virus-transformed indian muntjac cells, sr-mm-1, formed foci by murine sarcoma-xenotropic murine leukemia virus complex [msv(x-mulv)] superinfection. the response of sr-mm-1 and parental normal indian muntjac mm-2k cells to msv(x-mulv) infection was compared. focus formation by msv(x-mulv) followed two-hit kinetics in mm-2k, but one-hit kinetics in sr-mm-1 cells. msv(x-mulv)-infected sr-mm-1 cells formed larger colonies than uninfected sr-mm-1 cells in soft agar, while no colony wa ... | 1980 | 6247523 |
| factors affecting responses to murine oncogenic viral infections. | silica specifically kills macrophages in vitro, and in vivo has been used as a method of determining the possible immunological or other roles of macrophages in a number of viral infections. in experiments reported here, injection of 30 or 50 mg silica i.p. increased the severity of the oncogenic effects of the murine sarcoma virus (msv) and friend virus (fv) in balb/c mice. unlike herpes simplex and coxsackie b-3 infections, however, passive transfer of adult macrophages to suckling mice did no ... | 1980 | 6248095 |
| prostaglandin e1 binding and adenylate cyclase activation in normal and transformed fibroblasts. | the binding of [3h]prostaglandin e1 to membranes of clones of normal rat kidney fibroblasts (nrk cells) has been measured. cell lines that responded to prostaglandin e1, such as nrk and nrk transformed with schmitt-ruppin strain of rous sarcoma virus (sr-nrk cells), have a high affinity prostaglandin e1 binding site. murine-sarcoma-virus-transformed lines of nrk cells are unresponsive to prostaglandin e1 and have reduced prostaglandin e1 binding exposure of cells to prostaglandin e1 results both ... | 1980 | 6248120 |
| non-t-cell-mediated cytotoxicity in mice with tumors induced by moloney murine sarcoma virus (m-musv). ii. granulocyte-mediatd cytotoxicity against autochthonous target cells isolated from m-musv-induced tumors. | experiments were performed in a/sn and a/wysn mice to determine the specificity, organ distribution, and further characteristics of the small non-t-cells that infiltrate moloney murine sarcoma virus (m-musv)-induced tumors and are capable of killing the autochthonous m-musv-infected tumor target cells. continuous bovine serum albumin density gradient separation proved to be the most effective method of enriching the cytotoxic cells. increase in cytotoxic activity, measured by both the 51cr relea ... | 1980 | 6249952 |
| myeloproliferative virus, a cloned murine sarcoma virus with spleen focus-forming properties in adult mice. | myeloproliferative virus, derived from moloney sarcoma virus, causes erythroleukemia and myeloid leukemia in adult mice. this virus is also capable of fibroblast transformation in vitro. the virus consists of two separable biological entities which have been cloned. the helper virus component caused no visible changes in adult mice, whereas the defective virus induced both spleen focus formation and a large increase in erythroid precursor cells but retained the sarcoma virus property of transfor ... | 1980 | 6251235 |
| functional organization of the harvey murine sarcoma virus genome. | the comparative infectivity of harvey murine sarcoma virus (ha-musv) dna for nih 3t3 cells was determined for supercoiled ha-musv dna molecularly cloned in lambda phage and pbr322 at its unique ecori site (which is located near the middle of the 6-kilobase pair [kbp] unintegrated linear viral dna) and for two cloned subgenomic fragments: one was 3.8 kbp and lacked about 1 kbp from each side of the ecori site, and the second did not contain the 3 kbp of the unintegrated linear viral dna located o ... | 1980 | 6251279 |
| transforming growth factors: isolation of polypeptides from virally and chemically transformed cells by acid/ethanol extraction. | polypeptides characterized by their ability to confer a transformed phenotype on an untransformed indicator cell have been isolated directly from tumor cells growing both in culture and in the animal, by using an acid/ethanol extraction procedure. assay of these polypeptides is based on their ability to induce normal rat kidney fibroblasts to form colonies in soft agar. peptides from murine sarcoma virus-transformed mouse 3t3 cells grown in culture had the highest specific activity in this assay ... | 1980 | 6251462 |
| biological activity of cloned moloney sarcoma virus dna: terminally redundant sequences may enhance transformation efficiency. | we have measured the ability of cloned restriction fragments containing the whole and partial genomes of two strains of moloney murine sarcoma virus to induce cell transformation in dna transfection assays. the cloned intact ml and htl murine sarcoma virus proviruses transform with an efficiency of approximately 40,000-50,000 focus-forming units/pmol of proviral dna, and the majority of these transformed cells contain a rescuable viral genome. a cloned 2.1-kilobase-pair internal fragment of the ... | 1980 | 6251463 |
| isolation and partial characterization of a 55,000-dalton protein induced in cells transformed by the ml strain of moloney murine sarcoma virus. | 1980 | 6251602 | |
| the translation products of moloney murine sarcoma virus-124 rna. | 1980 | 6251612 | |
| organization of murine sarcoma virus genomes. | 1980 | 6253206 | |
| cellular origin of the transforming gene of moloney murine sarcoma virus. | 1980 | 6253207 | |
| characterization of virus-specified proteins present in nrk cells infected with a temperature-sensitive transformation mutant of moloney murine sarcoma virus. | 1980 | 6253209 | |
| kirsten murine sarcoma virus transformed cell lines and a spontaneously transformed rat cell-line produce transforming factors. | we have examined culture fluids from a variety of kirsten murine sarcoma virus (kimsv) transformed rat and mouse cells for the presence of factors which induce normal rat-1 cells to assume the transformed phenotype. all kimsv transformants produced transforming factor (tf). revertants of kimsv transformed rat or mouse failed to release tf as did normal rat or mouse cells. cells transformed by a temperature sensitive mutant of kimsv produced tf at the permissive temperature but not at the nonperm ... | 1980 | 6253509 |
| dual evolutionary origin for the rat genetic sequences of harvey murine sarcoma virus. | detailed restriction endonuclease maps were developed for harvey murine sarcoma virus (ha-musv) dna (clone h-1), molecularly closed at its unique ecori site in pbr322, for three nonoverlapping subgenomic hindiii clones which together span the entire h-1 clone and for a molecularly cloned dna copy of a portion of rat 30s rna (which represents the majority of the rat genetic sequences in ha-musv). molecular hybridization of the 30s clone to small restriction fragments of clone h-1 revealed a 0.9-t ... | 1980 | 6253666 |
| systemic and in situ natural killer activity in c57bl/6n and cba/n mice bearing murine sarcoma virus-induced regressor tumors. | both systemic and in situ natural killer (nk) activity was assessed in young and old c57bl/6n and cba/n mice bearing primary murine sarcoma virus-induced tumors. splenic nk activity was depressed in tumor-bearing mice relative to normal age-matched controls. in situ nk activity was detected in young mice, and the level paralleled that found in the spleens of the same mice. in older mice, in situ nk activity was not detected. furthermore, the depressed levels of activity in the spleens of tumor-b ... | 1980 | 6253708 |
| guanine nucleotide-binding and autophosphorylating activities associated with the p21src protein of harvey murine sarcoma virus. | the purified p21src protein of harvey sarcoma virus shows a guanine nucleotide-binding activity and, in addition, at elevated temperature an autophosphorylating activity at a threonine residue using as phosphoryl donor gtp or dgtp but not atp or datp. these biochemical activities are unique among those associated with transforming proteins of rna-containing or dna-containing tumour viruses. | 1980 | 6253810 |
| suppression of the leukocyte adherence inhibition response to murine-sarcoma-virus-induced tumors by tumor-associated suppressor cells. | the tumor-specific leukocyte adherence inhibition (lai) response of lymphoid cells from c57b1/6 mice sensitized to a murine-sarcoma-virus (musv)-induced tumor was inhibited by adherent phagocytic spleen cells from mice with progressively growing rbl-5 tumors. both the direct lai response and the formation of the mediator of lai were sensitive to the suppressive activity of the progressor spleen macrophages. suppression of lai was not restricted by h-2 differences between the sensitized lymphocyt ... | 1980 | 6253859 |
| mapping of transforming region of the harvey murine sarcoma virus genome by using insertion-deletion mutants constructed in vitro. | circular dna intermediates of harvey murine sarcoma virus (ha-musv) have been cloned in lambda gtwes . lambda b and shown to be capable of transforming mouse nih 3t3 cells [hager, g. l., chang, e. h., chan, h. w., garon, c. f., israel, m. a., martin, m. a., scolnick, e. m. & lowy, d. r. (1979) j. virol. 31, 795-809]. by using the cloned ha-musv dna insert as a parental genome, we have constructed a series of insertion-deletion mutants by inserting an octomer containing the sal i linker sequence ... | 1980 | 6254037 |
| nucleotide sequence analysis of the transforming region and large terminal redundancies of moloney murine sarcoma virus. | the sequence of the transforming region of the moloney murine sarcoma virus genome has been determined by using molecularly cloned viral dna. this region, 3.6 to 5.8 kilobase pairs from the left end of the molecule, contains the entire cellular insertion (src) sequence as well as helper viral sequences including the large terminal repeat (ltr). on the viral rna strand, a long (1224 bases) open reading frame commenced to the left of the src-helper virus junction and terminated at a point 58 nucle ... | 1980 | 6254069 |
| transforming growth factors produced by certain human tumor cells: polypeptides that interact with epidermal growth factor receptors. | three different human tumor lines in culture, a rhabdomyosarcoma, a bronchogenic carcinoma and a metastatic melanoma, release proteins (transforming growth factors, tgfs) into the medium that confer the transformed phenotype on untransformed fibroblasts. these proteins are acid and heat-stable; produce profound morphologic changes in rat and human fibroblasts; and enable normal anchorage-dependent cells to grow in agar. removal of the transforming protein results in a reversion of cell phenotype ... | 1980 | 6254071 |
| transformation by cloned harvey murine sarcoma virus dna: efficiency increased by long terminal repeat dna. | the coding sequences for the transforming (src) protein (p21) of harvey murine sarcoma virus have been localized to a 1.3 kilobase pair segment near the 5' end of the viral genome. ligation of the viral terminal repeat dna to the left end of the src region dna markedly enhanced the low transforming efficiency of the src region dna. | 1980 | 6254153 |
| identification of a normal vertebrate cell protein related to the p21 src of harvey murine sarcoma virus. | 1980 | 6254252 | |
| multinucleation in the presence of cytochalasin b by rna tumor virus-transformed cells. | rna tumor virus-transformed cell cultures derived from rat, mouse, hamster, and mink were examined for their response to cytochalasin b (cb), and the expression of this marker was correlated with growth in soft agar and tumorigenicity in vivo. continuous cell lines transformed and chronically infected with moloney murine sarcoma-leukemia virus (m-msv-mulv) or kirsten murine sarcoma-leukemia virus were extensively multinucleated when treated with cb. similarly, nonproducer moloney murine sarcoma ... | 1980 | 6254645 |
| simian virus 40 and moloney-murine sarcoma virus infection of bona fide mouse epithelium. | moloney-murine sarcoma virus (moloney-msv) and simian virus 40 (sv40) were found to infect successfully pure cultures of epithelial cells established from mouse liver and mammary tissue. msv infection resulted in transient morphological foci with persistent production of infectious virus. sv40 infection produced detectable levels of virus-specific t antigen in the cells but morphological transformants were not observed. | 1980 | 6255079 |
| frequent site-specific deletion of coliphage lambda murine sarcoma virus recombinants and its use in the identification of a retrovirus integration site. | stocks of hybrid lambda phages carrying the complete integrated provirus of either m1 or ht1 moloney murine sarcoma virus, as well as flanking host sequences, frequently contain significant numbers of phages carrying a specific deletion. this deletion arises from a recombination event between the terminally repeated sequences in the provirus that deletes the unique moloney murine sarcoma virus sequences bracketed by the terminally repeated sequences. physical mapping has shown that the deletion ... | 1980 | 6255187 |
| lack of focus formation of s+l- mink cells by the ecotropic murine leukemia viral genome. | infection of mink s+l- cells with ecotropic murine leukemia virus, achieved by phenotypic mixing with xenotropic virus, did not result in the induction of transformed foci. also, clonal line of s+l- mink cells, chronically infected with ecotropic murine leukemia virus, which produce both the ecotropic virus and its murine sarcoma virus pseudotype are morphologically indistinguishable from normal s+l- mink cells. neutralizing antiserum added to s+l- mink cells inoculated with xenotropic virus 24 ... | 1980 | 6255204 |
| heteroduplex analysis of cloned rat endogenous replication-defective (30 s) retrovirus and harvey murine sarcoma virus. | 1980 | 6255681 | |
| moloney murine sarcoma proviral dna is a transcriptional unit. | a portion of moloney murine sarcoma virus dna which is repeated at both ends of the provirus has been sequences. the nucleotide sequence, together with hybridization data obtained with in vitro pulse-labelled nascent viral rna, indicate that initiation and termination of rna synthesis occur within that region of the proviral dna. a model for transcriptional readthrough of termination signals during rna synthesis in this system is suggested. | 1980 | 6256637 |
| biological, immunological, and molecular properties of revertants of cat cells transformed by murine sarcoma virus. | 1981 | 6257388 | |
| virus rna species in kirsten murine sarcoma virus-transformed mink cells. | the nuclear and cytoplasmic rnas from kirsten murine sarcoma virus (kimusv)-transformed non-producer mink cells were studied for the species of virus-specific rna by fractionation in agarose gels, transfer to diazotized aminophenylthioether paper and hybridization to complementary dna probe. in both nuclei and cytoplasm, only genome-length kimusv-specific rna was detected. no subgenomic virus rna species was detected in poly(a+) or poly(a-) rna fractions. the same observations were made in kimus ... | 1980 | 6257834 |
| structure of and alterations to defective murine sarcoma virus particles lacking envelope proteins and core polyprotein cleavage. | htg2 hamster cells produce a defective murine sarcoma virus lacking gp70 and, consequently, viral surface projections (knobs), but the lack of knobs appears to have no effect on intramembrane particle distribution. in addition, it has been noted that the core of the virus remains in the "immature" form as a result of the failure of the polyprotein precursor (p65) to undergo cleavage. however, incubation of htg2 virus with avian myoblastosis virus was found to yield specific cleavage products of ... | 1980 | 6257930 |
| onc sequences (v-fes) of snyder-theilen feline sarcoma virus are derived from noncontiguous regions of a cat cellular gene (c-fes). | type c sarcoma viruses are genetic recombinants containing portions of replication-competent helper viruses linked to sarcoma virus-specific sequences (generically designated onc genes) which are thought to be required for acute fibroblast transformation. the onc elements of different avian and mammalian sarcoma viral isolates are each homologous to subsets of cellular dna sequences which have no well-defined role in normal cells. because of the lack of significant homology between helper viral ... | 1981 | 6259536 |
| morphological and functional differentiation of kirsten murine sarcoma virus-transformed rat adrenocortical cell lines. | this study was undertaken to examine relationships of the phenotype of malignant cells to target cell properties and to events early in the transformation process. eighteen transformed lines were obtained by kirsten murine sarcoma virus infection of cells from adrenal glands of rats ages 4 to 30 weeks, at first or second passages in culture. they were grown either as fibroblastic adrenocortical stem cells or as more differentiated epithelial cells, depending on culture conditions. of 14 lines ex ... | 1981 | 6260350 |
| guanine nucleotide binding activity of the src gene product of rat-derived murine sarcoma viruses. | 1980 | 6261655 | |
| avian reticuloendotheliosis virus: characterization of genome structure by heteroduplex mapping. | the genome structure of defective, oncogenic avian reticuloendotheliosis virus (rev) was studied by heteroduplex mapping between the full-length complementary dna of the helper virus rev-t1 and the 30s rev rna. the rev genome (5.5 kilobases) had a deletion of 3.69 kilobases in the gag-pol region, confirming the genetic defectiveness of rev. in addition, rev lacked the sequences corresponding to the env gene but contained, instead, a contiguous stretch (1.6 to 1.9 kilobases) of the specific seque ... | 1981 | 6262534 |
| transformation of cultured rat ovarian surface epithelial cells by kirsten murine sarcoma virus. | a method is described for the culture of rat ovarian surface epithelial cells, i.e., the cellular component thought to be the source of most ovarian cancers. these cells in culture have a characteristic epithelial morphology which distinguishes them from other ovarian cell types. cultured surface epithelial cells are histochemically positive for 17 beta-hydroxysteroid dehydrogenase and negative for delta 5-3 beta-hydroxysteroid dehydrogenase, the same as in cryostat sections of whole rat ovary. ... | 1981 | 6263459 |
| translational products of moloney murine sarcoma virus rna: identification of proteins encoded by the murine sarcoma virus src gene. | in vitro translation of virion rna of moloney murine sarcoma virus (msv) strain 124 yielded major products having molecular weights of 63,000 (63k), 43k, 40k, 31k, and 24k daltons. a molecularly cloned subgenomic fragment of moloney msv comprised of the cellular insertion (src) region was utilized in hybridization arrest translation as a means of identifying products of the msv src gene. msv src dna specifically inhibited synthesis of the 43k, 40k, 31k, and 24k proteins, implying that each of th ... | 1981 | 6264137 |
| structure and functions of the kirsten murine sarcoma virus genome: molecular cloning of biologically active kirsten murine sarcoma virus dna. | the unintegrated closed circular form of viral dna prepared from nih3t3 cells infected with kirsten murine sarcoma virus was cloned into bacterial plasmid pbr322. the closed circular dna, which consisted of two different-sized populations, was enriched from the virus-infected cells, linearized with bamhi, and inserted into pbr322 dna. four different recombinant dnas (clones 2, 4, 6, and 7) were obtained, and a physical map of each was constructed by using various restriction enzymes. clone 4 dna ... | 1981 | 6264139 |
| identification of unintegrated forms of kirsten murine sarcoma viral dna and restriction endonuclease cleavage map of linear dna. | we detected unintegrated linear 7.0-kilobase pair dna and covalently closed circular dna species in nih3t3 cells recently infected with kirsten murine sarcoma virus. using the linear dna, we constructed a restriction endonuclease cleavage map and compared it with the map of harvey murine sarcoma virus. the restriction endonuclease maps of two segments, one 1.2 kilobase pairs (smai site) to 3.7 kilobase pairs (hindiii site) from the right end (corresponding to the viral 3' side) and the other 0.5 ... | 1981 | 6264147 |
| regulation of cell-mediated immunologic reactivity to moloney murine sarcoma virus-induced tumors. i. cell and serum activity detected by leukocyte adherence inhibition. | cell-mediated immunity and serum regulatory factors were studied in an in vitro system involving a spontaneously regressing, virus-induced tumor. inbred balb/c and cba mice were inoculated with moloney murine sarcoma virus and their peritoneal cells were tested for reactivity in leukocyte-adherence inhibition tests with extracts of syngeneic tumors. sera from inoculated mice were tested for their effect on this reactivity. at the optimal dilution, tumor extracts induced significant reactions wit ... | 1981 | 6264193 |
| three distinct genes in human dna related to the transforming genes of mammalian sarcoma retroviruses. | southern blot hybridization was used to identify human and other vertebrate dna sequences that were homologous to cloned dna fragments containing the oncogenic nucleic acid sequences of three different type c mammalian retroviruses (simian sarcoma virus, the snyder-theilen strain of feline sarcoma virus, and the harvey strain of murine sarcoma virus). each onc gene counterpart has a single genetic locus, which probably contains non-onc intervening sequences. the human dna sequences may represent ... | 1981 | 6264598 |
| enhancement of antibody activity to bovine leukemia virus by complement. | cocultivation of fetal lamb kidney cells infected with bovine leukemia virus (blv) and the cat cells containing murine sarcoma virus genome resulted in the rapid production of syncytia. this syncytia formation was inhibited by serum containing antibodies to glycoprotein antigen of blv. when rabbit complement was added to the antiserum for early syncytia inhibition (esi) test, a significant enhancement of esi activity of the antiserum was observed. this enhancement was associated with igg fractio ... | 1981 | 6264895 |
| transforming growth factor production by chemically transformed cells. | evidence is presented indicating that the chemically transformed akr-mca and c3h/mca-58 murine cell lines produce "transforming growth factor(s)" capable of inducing a transformed morphology and the ability to grow in soft agar in nontransformed, anchorage-dependent indicator cells. serum-free medium conditioned by exposure to the chemically transformed cells was chromatographed on a bio-gel p-60 column after dialysis and lyophilization. using the nontransformed mouse akr-2b cells as the indicat ... | 1981 | 6265069 |
| in vivo antigenic modification of tumor cells. iii. metastatic thymic lymphoma specifically infected by thymotropic retrovirus. | tissue distribution of radiation leukemia virus (radlv) was examined after its inoculation into normal c57bl/6j (b6) mice, b6 mice bearing a transplantable, non-virus-producing thymic lymphoma (rl12-np), and b6 mice bearing a transplanted non-virus producing, harvey murine sarcoma virus-transformed fibrosarcoma. virus expression was determined by competition radioimmunoassay for murine leukemia virus (mulv) p30 (predominant group-reactive antigen of mulv) and for radlv p12 (a highly type-specif ... | 1981 | 6265678 |
| the p21 src genes of harvey and kirsten sarcoma viruses originate from divergent members of a family of normal vertebrate genes. | the harvey and kirsten strains of murine sarcoma virus encode enzymatically and serologically related p21 src proteins which are required for virally mediated cellular transformation. the genes in each virus encoding p21 show such extensive divergence from each other that cloned probes from these genes detect distinct sets of cellular genes in the dna from several vertebrate species. these data suggest that cellular p21 sarc genes constitute a divergent family of vertebrate genes that can regula ... | 1981 | 6265801 |
| host range of murine sarcoma virus, its host modification and phenotypic mixing with endogenous virus. | the m(msv)ag-50 cells produce competent ecotropic sarcoma virus which is able to transform several rodent embryo cells and replicate in them. the expanded host range has shown that the virus acquired some host information which facilitate the transformation of heterologous cells. the transformed cells were xc positive and with transformed phenotype in vitro. the genome of mouse sarcoma virus from nonproducer rat liver cells could be rescued by xenotropic endogenous virus. the obtained virus has ... | 1981 | 6265817 |
| migration of null lymphocytes to murine sarcoma virus-induced tumors. | 1981 | 6266725 | |
| transforming growth factors (tgfs): properties and possible mechanisms of action. | transforming growth factors (tgfs) are growth-promoting polypeptides that cause phenotypic transformation and anchorage-independent growth of normal cells. they have been isolated from several human and animal carcinoma and sarcoma cells. one tgf is sarcoma growth factor (sgf) which is released by murine sarcoma virus-transformed cells. the tgfs interact with epidermal growth factor (egf) cell membrane receptors. tgfs are not detectable in culture fluids from cells which contain high numbers of ... | 1981 | 6267317 |
| analysis of two divergent rat genomic clones homologous to the transforming gene of harvey murine sarcoma virus. | harvey murine sarcoma virus (ha-musv) is a mouse-rat recombinant retrovirus that encodes a protein designated p21, required for virally induced transformation. using a radiolabeled dna fragment from the p21 coding region, we have detected homologous dna sequences in the normal dna of rats and of several other vertebrate species. moreover, many tested cells from these species contain low levels of a p21 protein that is highly related to viral 21. now we report two independent fragments from norma ... | 1981 | 6267583 |
| increased sensitivity of fibroblasts of skin from patients with adenomatosis coli and peutz-jegher's syndrome to transformation by murine sarcoma virus. | skin fibroblasts from family members of cases of adenomatosis coli (ac) and peutz-jegher's syndrome (pj) were examined for susceptibility to morphological transformation by moloney murine sarcoma virus (msv) of rate 78ai cells, which was found in the present study to contain both dualtropic and ecotropic murine leukemia virus (mlv). the fibroblasts from individuals with manifest ac (22 subjects) and manifest pj (5 subjects) were up to 5 times more susceptible to transformation by rat 78ai-msv(ml ... | 1980 | 6268477 |
| neoplastic transformation of mouse fibroblasts by murine sarcoma virus: a multi-step process. | infection of cultures of three different normal rodent cell lines with murine sarcoma virus (msv) resulted in very rapid loss of contact inhibition of growth and morphological transformation. in the case of two of these lines, anchorage dependence of growth was also rapidly lost but with the 3rd, c3h10t1/2 c1.8, an established line of mouse embryo fibroblasts, there was a delay of many cell generations before the cells became anchorage independent. this was despite 100% successful infection, as ... | 1981 | 6268740 |
| ldhk, a uniquely regulated cryptic lactate dehydrogenase associated with transformation by the kirsten sarcoma virus. | a novel isozyme of lactate dehydrogenase is detected in various cells transformed by the kirsten murine sarcoma virus (kimsv). this isozyme, designated ldhk, is strongly inhibited by physiological concentrations of oxygen, in an apparently cooperative fashion. ldhk is inhibited by guanosine triphosphate and related compounds, in a noncompetitive fashion. ldhk is found with both 35,000- and 22,000-dalton subunits, although these probably cleave from a 57,000-dalton precursor. in studies utilizing ... | 1981 | 6270118 |
| construction and isolation of a transmissible retrovirus containing the src gene of harvey murine sarcoma virus and the thymidine kinase gene of herpes simplex virus type 1. | we constructed lambda recombinants containing the harvey murine sarcoma virus genome and the thymidine kinase (tk) gene of herpes simplex virus type 1 linked to each other. the tk gene was located in a position downstream from both the long terminal repeat and the src gene of harvey murine sarcoma virus. the dnas of the lambda recombinants were used to transfect nih3t3 mouse fibroblasts in order to obtain harvey murine sarcoma virus dna-induced foci of transformed cells. the transformed foci wer ... | 1981 | 6270359 |
| specific transcriptional initiation in vitro on murine type c retrovirus promoters. | we have investigated the ability of molecularly cloned murine type c retroviral dna to direct accurate initiation of rna synthesis when added to cell-free extracts. two different cloned proviruses were used. the first was derived from an integrated molecule of akr murine leukemia virus and contains adjacent host information. the origin of the second was an unintegrated permuted copy of harvey murine sarcoma virus. we found that the leukemia virus cloned provirus, as predicted by structural consi ... | 1981 | 6270679 |
| transformation of rat thyroid epithelial cells by kirsten murine sarcoma virus. | fischer rat thyroid epithelial cella (frt) growing continuously in culture were infected with the kirsten murine sarcoma virus kimsv(kimulv) and found to produce this virus constitutively. although the morphology of the frt cells did not change appreciably, the cells became malignant after infection with kimsv(kimulv) as shown by the growth of infected cels in semi-solid media (uninfected frt cells did not grow) and by the tumorigenicity of infected when injected into syngeneic animals (uninfect ... | 1981 | 6273340 |
| sv40 recombinant molecules express the gene encoding p21 transforming protein of harvey murine sarcoma virus. | 1981 | 6273733 | |
| mechanisms of kirsten murine sarcoma virus transformation-induced changes in the collagen phenotype and synthetic rate of balb 3t3 cells. | specific viral transformation rather than cell selection can explain the previously observed increase in the proportion of type iii procollagen compared to type i procollagen in balb 3t3 cells transformed by kirsten murine sarcoma virus (ki-msv). two subclones of balb 3t3 a31 were productively infected with with a temperature-sensitive ki-msv in the presence of helper murine leukemia virus (mlv), resulting in virtually complete transformation of cultures and eliminating selection of transformed ... | 1981 | 6273840 |
| mouse embryonic transforming growth factors related to those isolated from tumor cells. | growth factors with the two characteristic properties of sarcoma growth factor, the ability to stimulate anchorage-independent growth of normal mouse or rat fibroblasts and the ability to compete with 125i-labeled epidermal growth factor for receptor binding, can be isolated from 12- to 13-day-old normal mouse embryos of various strains. two size classes of the transforming factor from embryo cells can be isolated with apparent molecular weights of 20,000 and 10,000. the lower-molecular-weight f ... | 1982 | 6275982 |
| block in the expression of differentiation markers of rat thyroid epithelial cells by transformation with kirsten murine sarcoma virus. | well-differentiated epithelial cells, derived from primary cultures of normal rat thyroid glands (t-79 cells), as well as a cloned cell line also derived from normal rat thyroid glands (frt-l cells) were infected with kirsten murine sarcoma virus carrying outer coat of the helper kirsten murine leukemia virus. infected t-79 and frt-l cells changed morphologically and began to proliferate rapidly, suggesting malignant transformation by the virus. both cell lines can support the replication of bot ... | 1982 | 6275984 |
| generation of balb-musv and ha-musc by type c virus transduction of homologous transforming genes from different species. | the nature of the cell-derived (bas) sequences of balb-musv, a spontaneous mouse sarcoma virus isolate, was determined. molecularly cloned bas sequences demonstrated no detectable homology with the onc genes of other mouse transforming viruses, but exhibited a high degree of sequence homology with the ras gene of the rat-derived harvey murine sarcoma virus (ha-musv) genome. the ha-musv cell-derived sequence (ras) shared a colinear 750 bp region of homology with bas. moreover, balb-musv transform ... | 1981 | 6276008 |
| harvey and kirsten sarcoma viruses promote the growth and differentiation of erythroid precursor cells in vitro. | harvey and kirsten murine sarcoma viruses have previously been shown to transform fibroblastic cells in culture, and type c virus pseudotypes of these viruses cause erythroleukemia in susceptible mice. we report a cell culture assay for quantitating the growth-promoting effect of harvey and kirsten viruses on erythroid cells. murine hemopoietic cells were infected in vitro with harvey or kirsten sarcoma virus, and then cultured in methylcellulose in the presence of relatively low concentrations ... | 1981 | 6276011 |
| analysis of transforming gene products from moloney murine sarcoma virus. | we previously showed that in vitro translation of m-musv virion rna yielded a 62 kd gag gene product and an overlapping set of four proteins with approximate molecular weights of 37,000, 33,000, 24,000 and 18,000. in this paper we show, by use of hybrid arrest translation with cloned recombinant dnas containing m-musv v-mosmo sequences, that the 37, 33, 24 and 18 kd proteins are synthesized in their entirety from the v-mosmo gene. analysis of the primary sequence of these proteins shows that eac ... | 1981 | 6276018 |
| activation of sv40 genome by 72-base pair tandem repeats of moloney sarcoma virus. | the simian virus 40 (sv40) 72-base pair (bp) tandem-repeated sequences have a crucial role as an activator element in viral gene expression. we replaced the sv40 72-bp repeat with a 72-bp repeat derived from the long terminal repeat (ltr) of cloned moloney murine sarcoma virus (msv) dna. although there is no detectable sequence homology to sv40, the msv repeats can substitute functionally for the sv40 repeats and generate a viable virus in monkey kidney cells. | 1982 | 6276775 |
| pericellular matrix and cell surface glycoproteins of virus-transformed mouse epithelial cells. | mouse embryo mus musculus castaneous epithelial cells, transformed with moloney murine sarcoma virus (msv) or with ecotropic murine leukemia virus (mulv), were analyzed for production of pericellular matrix glycoproteins. the nontransformed, msv-transformed, and mulv-transformed cells produced fibronectin, laminin, type i collagen, and small amounts of type iii collagen when studied by immunofluorescence using specific antibodies. the virus-transformed epithelial cells produced enhanced amounts ... | 1982 | 6277476 |
| glucocorticoid regulation of the ha-musv p21 gene conferred by sequences from mouse mammary tumor virus. | molecular chimeras with the p21 transforming gene of harvey murine sarcoma virus linked to dna containing the long terminal repeat (ltr) or mouse mammary tumor virus (mmtv) have been constructed. transformants of nih 3t3 cells induced by transfection with mmtv ltr-p21 hybrid dna have been identified that express the normal p21 gene product. the levels of p21 rna and protein in these transformants are regulated by physiological concentrations of dexamethasone, a synthetic glucocorticoid hormone. ... | 1981 | 6277498 |
| expression of transforming region of moloney murine sarcoma virus in escherichia coli as a fusion protein with small tumor antigen of polyoma virus. | bacterial expression of the transforming region of moloney murine sarcoma virus, designated mos, was obtained as a fusion protein with a portion of the small tumor antigen of polyoma virus. this was accomplished by fusing the entire mos open reading frame, encoding a 41,000-dalton protein, with a plasmid that expresses a beta-galactosidase-polyoma fusion protein under lac operon control. the resulting plasmid directed synthesis of the predicted polyoma antigen-sarcoma virus fusion protein of 59, ... | 1982 | 6278495 |
| resistance to moloney murine sarcoma virus (m-musv) tumor induction is associated with natural antibody production to "endogenous" moloney leukemia virus (m-mulv) in balb/mo mice. | balb/mo mice are characterized by early expression of "endogenized" m-mulv and are resistant to m-musv tumor induction. furthermore, compared to normal balb/c mice, sera from balb/mo mice exhibit a significant reactivity which is specific for m-mulv when tested in a 125i-labelled staphylococcus protein a binding assay. the possible significance of this reactivity in conferring tumor resistance is explored. | 1981 | 6278685 |
| moloney murine sarcoma virus tumors in cba/j mice: chemopreventive and chemotherapeutic actions of supplemental beta-carotene. | decreased tumor frequency, increased latent period, and increased rate of tumor regression were observed in male inbred cba/j mice fed supplemental beta-carotene before and/or after they were inoculated with the moloney sarcoma virus. when beta-carotene feeding was begun after tumors were already present, it markedly increased the rate of tumor regression. beta-carotene minimized the virus-induced thymus gland involution that accompanies tumor growth, and this action on the thymus gland was beli ... | 1982 | 6279952 |
| human nucleotide sequences related to the transforming gene of a murine sarcoma virus: studies with cloned viral and cellular dnas. | a recombinant plasmid, pi26, has been constructed by cloning into pbr322 a transforming gene of murine sarcoma virus (a moloney strain, clone 124, msv) synthesized by detergent-treated virions. from this plasmid a xbai-hindiii fragment has been isolated which contains only mos-specific sequences. this mos-specific probe has been used for screening a human gene library cloned in bacteriophage lambda charon 4a. of these, 19 clones have been isolated containing mos-related sequences. by physical ma ... | 1982 | 6281133 |
| long terminal repeat of murine retroviral dnas: sequence analysis, host-proviral junctions, and preintegration site. | the nucleotide sequence of the long terminal repeat (ltr) of three murine retroviral dnas has been determined. the data indicate that the u5 region (sequences originating from the 5' end of the genome) of various ltrs is more conserved than the u3 region (sequences from the 3' end of the genome). the location and sequence of the control elements such as the 5' cap, "tata-like" sequences, "ccaat-box," and presumptive polyadenylic acid addition signal aataaa in the various ltrs are nearly identica ... | 1982 | 6281466 |
| a generalized method of subcloning dna fragments by restriction site reconstruction: application to sequencing the amino-terminal coding region of the transforming gene of gazdar murine sarcoma virus. | the technique of restriction site reconstruction was generalized so as to allow the subcloning of any dna fragment and its subsequent reexcision with ecori, xbai, xhoi or hindiii. after excision, the 3' terminus of each strand will be derived from the starting nucleic acid, permitting the use of such fragments as primers for nucleotide sequencing by primer extension methods. the technique was used to subclone a 56 base pair bstni-ddei fragment of moloney murine sarcoma virus (mo-msv) as a unique ... | 1982 | 6281735 |
| candidate product of the fbj murine osteosarcoma virus oncogene: characterization of a 55,000-dalton phosphoprotein. | sera from rat bearing tumors induced by inoculation of fbj murine osteogenic sarcoma virus (fbj-msv) nonproducer rat cells precipitate two proteins with molecular weights of 55,000 (p55) and 39,000 (p39) from fbj-msv-transformed cells. these proteins cannot be precipitated from uninfected cells or cells transformed by other strains of murine sarcoma virus, nor can they be precipitated by sera specific for the viral structural proteins. a methionine tryptic peptide mapping analysis showed that p5 ... | 1982 | 6283132 |
| identification of a precursor in the biosynthesis of the p21 transforming protein of harvey murine sarcoma virus. | the p21 transforming protein coded for by the v-ras gene of harvey murine sarcoma virus (ha-musv) migrates as a doublet band between 21,000 and 23,000 daltons during sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the lower band of the doublet is designated p21, and the upper band is designated pp21 since it comigrates with the phosphorylated form of p21. by pulse-labeling with [35s] methionine, we detected a p21 precursor, pro-p21, which migrated as if it was approximately 1,000 dalt ... | 1982 | 6283143 |
| tumorigenic transformation of mammalian cells induced by a normal human gene homologous to the oncogene of harvey murine sarcoma virus. | a normal human gene homologous to the p21 ras oncogene of harvey murine sarcoma virus induced oncogenic transformation and high p21 ras levels in murine fibroblasts when this gene was ligated to a control element (the long terminal repeat) from a murine or feline retrovirus. these results indicate that high levels of a gene product encoded by a normal human oncogene can induce tumorigenic transformation. | 1982 | 6283358 |
| t24 human bladder carcinoma oncogene is an activated form of the normal human homologue of balb- and harvey-msv transforming genes. | a transforming gene isolated from t24 human bladder carcinoma cells is closely related to the balb murine sarcoma virus (msv) onc gene (v-bas). this transforming gene is localized to a 4.6 kilobase pair (kbp) region and is expressed as a 1.2-kbp polyadenylated transcript, which contains v-bas related sequences. moreover, antisera known to detect the immunologically related onc gene products of balb- and harvey-msvs recognized elevated levels of a related protein in t24 cells. the normal human ho ... | 1982 | 6283384 |
| expression of cellular homologues of retroviral onc genes in human hematopoietic cells. | total cellular poly(a)-enriched rna from a variety of fresh human leukemic blood cells and hematopoietic cell lines was analyzed for homology with molecularly cloned dna probes containing the onc sequence of abelson murine leukemia virus (ab-mulv), harvey murine sarcoma virus (ha-musv), simian sarcoma virus (ssv), and avian myelocytomatosis virus strain mc29. results with the fresh blood cells paralleled those obtained with the cell lines. with ab-mulv and ha-musv, multiple rna bands were visual ... | 1982 | 6283530 |
| viral src gene products are related to the catalytic chain of mammalian camp-dependent protein kinase. | the transforming protein sequences translated from the rous avian and moloney murine sarcoma virus src genes are shown to be related to the catalytic chain of bovine camp-dependent protein kinase (atp:protein phosphotransferase, ec 2.7.1.37). the avian transforming protein, also a protein kinase, shows greatest homology with the bovine protein kinase in the carboxyl-terminal half, where the protein kinase activity is localized. moreover, lysine occurs in the inferred transforming protein sequenc ... | 1982 | 6283546 |
| induction of sarcomas in athymic mice. | during the course of serial passage of 50 human xenografts in the athymic mouse over a period of 5 years we have observed two cases of induction of sarcomas in the murine stromal tissue associated with the human xenografts. both times the growth of the murine sarcomas overtook that of the human xenograft. this change was monitored by analysis of the lactate dehydrogenase isozyme profile and histology of each passage of the human xenografts in the athymic mice. the two murine sarcomas were subseq ... | 1982 | 6283553 |
| nucleotide sequence analysis of the long terminal repeat of integrated simian sarcoma virus: evolutionary relationship with other mammalian retroviral long terminal repeats. | nucleotide sequence analysis of the long terminal repeat (ltr) of the integrated simian sarcoma virus showed that the simian sarcoma virus ltr comprised 504 nucleotides with an inverted repeat of seven bases at its 5' and 3' termini. at the site of simian sarcoma virus integration, cellular flanking sequences adjacent to the proviral ltr contained a direct repeat of four bases. a 13-base sequence after the 5' ltr was found to be complementary to prolyl trna, suggesting that trnapro may serve as ... | 1982 | 6284990 |
| balb- and harvey-murine sarcoma virus transformation of a novel lymphoid progenitor cell. | balb- and harvey-murine sarcoma viruses (msv) comprise a family of retroviruses whose mouse- and rat-derived onc genes are closely related. these viruses induce sarcomas and erythroleukemias in susceptible animals. an in vitro colony assay that detects transformation of lymphoid cells by abelson-murine leukemia virus was used to demonstrate that balb- and harvey-msv transform a novel hematopoietic cell both in culture and in vivo. bone marrow colony formation was sarcoma virus dependent, followe ... | 1982 | 6286838 |
| monoclonal antibodies to the p21 products of the transforming gene of harvey murine sarcoma virus and of the cellular ras gene family. | we have isolated eight rat lymphocyte-myeloma hybrid cell lines producing monoclonal antibodies that react with the 21,000-dalton transforming protein (p21) encoded by the v-ras gene of harvey murine sarcoma virus (ha-musv). these antibodies specifically immunoprecipitate both phosphorylated and non-phosphorylated forms of p21 from lysates of cells transformed by ha-musv. all eight react with the products of closely related ras genes expressed in cells transformed by two additional sarcoma virus ... | 1982 | 6287003 |
| cellular moloney murine sarcoma (c-mos) sequences are hypermethylated and transcriptionally silent in normal and transformed rodent cells. | moloney murine sarcoma virus carries an oncogenic sequence (v-mos) which is homologous to a single copy gene (c-mos) present in the normal cells of several vertebrate species. because of the possible significance of c-mos sequences in normal development and malignant transformation induced by physical or chemical agents, we have examined the state of integration, methylation, and transcriptional activity of c-mos sequences in a variety of normal rodent tissues, normal cell lines, or cell lines t ... | 1982 | 6287220 |
| human dna sequence homologous to the transforming gene (mos) of moloney murine sarcoma virus. | we describe the molecular cloning of a 9-kilo-base-pair bamhi fragment from human placental dna containing a sequence homologous to the transforming gene (v-mos) of moloney murine sarcoma virus. the dna sequence of the homologous region of human dna (termed humos) was resolved and compared to that of the mouse cellular homolog of v-mos (termed mumos) [van beveren, c., van straaten, f., galleshaw, j.a. & verma, i.m. (1981) cell 27, 97-108]. the humos gene contained an open reading frame of 346 co ... | 1982 | 6287464 |
| nucleotide sequence of the p21 transforming protein of harvey murine sarcoma virus. | harvey murine sarcoma virus is a retrovirus which transforms cells by means of a single virally encoded protein called p21 has. we have determined the nucleotide sequence of 1.0 kilobase in the 5' half of the viral genome which encompasses the has coding sequences and its associated regulatory signals. the nucleotide sequence has identified the amino acid sequence of two additional overlapping polypeptides which share their reading frames and the carboxyl termini with p21 but which contain addit ... | 1982 | 6287572 |
| nucleotide sequence of the oncogene encoding the p21 transforming protein of kirsten murine sarcoma virus. | the transforming protein ofkirsten murine sarcoma virus (ki-musv) is a virally encoded 21-kilodalton protein called p21 kis. the sequences encoding p21 kis were genetically localized to a 1.3-kilobase segment near the 5' end of the viral genome by assaying the capacity of a series of defined deletion mutants of molecularly cloned ki-musv dna to induce focal transformation of mouse cells. nucleotide sequencing of a portion of this region has led to the identification of an open reading frame of 5 ... | 1982 | 6287573 |
| detection of a transforming gene product in cells transformed by moloney murine sarcoma virus. | we identified, in cells transformed by moloney murine sarcoma virus (m-musv clone 124), a protein encoded by the m-musv transforming gene, v-mos. an antiserum against a synthetic peptide corresponding to the c terminus of a protein predicted from the v-mos nucleotide sequence specifically recognizes a protein doublet of approximately 37,000 daltons from 35s-methionine-labeled m-musv 124-transformed producer cells. by peptide mapping, this protein is almost identical to the 37 kd in vitro transla ... | 1982 | 6288258 |
| characterization of the phosphorylation sites and the surrounding amino acid sequences of the p21 transforming proteins coded for by the harvey and kirsten strains of murine sarcoma viruses. | the transforming protein coded for by the onc gene (v-rasha) of harvey murine sarcoma virus (ha-musv) is the 21,000-dalton protein (p21) which is the immediate agent responsible for the virus-induced malignant transformation of normal cells. the p21 proteins of ha-musv and the closely related kirsten murine sarcoma virus are heavily phosphorylated in vivo. in the partially purified ha-musv p21, the protein shows a guanine nucleotide-binding activity and, in addition, a very unique autophosphoryl ... | 1982 | 6288698 |
| murine xenotropic type c viruses. iv. replication and pathogenesis of ducks. | the xenotropic (x-tropic) mouse type c virus (mulv) and its pseudotype of murine sarcoma virus (msv) were inoculated into several fertilized developing pekin duck eggs. the development of the duck embryos was substantially reduced in those receiving the x-tropic viruses compared to eggs inoculated only with tissue culture medium. infections virus was isolated from some of the adult animals; in others, evidence for integrated virus sequences in the tissues was noted. no specific pathology was fou ... | 1982 | 6288852 |
| autocrine secretion of peptide growth factors by tumor cells. | transforming growth factors (tgf) are growth-promoting polypeptides that cause phenotypic transformation and anchorage-independent growth of normal cells. they have been isolated from several human and animal carcinoma and sarcoma cells. one tgf is sarcoma growth factor (sgf) which is released by murine sarcoma virus-transformed cells. whereas the tgf interacts with epidermal growth factor (egf) cell membrane receptors, it is not detectable in culture fluids from cells which contain high numbers ... | 1982 | 6289103 |
| human genome contains four genes homologous to transforming genes of harvey and kirsten murine sarcoma viruses. | harvey and kirsten murine sarcoma viruses each encode a structurally and functionally related 21-kilodalton protein (p21), which is the transforming protein of each virus. using probes from the 0.9-kilobase (kb) p21-coding region of each virus (called v-ha-ras and v-ki-ras, respectively), we have molecularly cloned from normal human genomic dna the sequences that hybridize to these probes. four evolutionarily divergent restriction endonuclease fragments were isolated. two hybridized preferential ... | 1982 | 6289320 |
| kirsten murine sarcoma virus-coded p21ras may act on multiple targets to effect pleiotropic changes in transformed cells. | 1982 | 6289520 | |
| comparison of complexes containing lysyl-trna synthetase from normal and virus-transformed cells. | we compared the lysyl-trna synthetases from normal (balb/3t3) and murine sarcoma virus-transformed (ka31) mouse fibroblasts. in agreement with several other reports of mammalian systems, the lysyl-trna synthetases from these cells occurred in very large postmicrosomal complexes as determined by gel filtration on agarose columns. arginyl-, isoleucyl-, methionyl-, phenylalanyl-, and tyrosyl-trna synthetases also occurred as part of a large complex or complexes. activity of glycyl- or leucyl-trna s ... | 1982 | 6290008 |
| synergistic interaction of two classes of transforming growth factors from murine sarcoma cells. | transforming growth factors (tgfs) isolated from murine sarcoma virus-transformed 3t3 cells have been separated by high-pressure liquid chromatography into two subsets. one subset, called tgf alpha, competes with epidermal growth factor (egf) for receptor sites, whereas the other, called tgf beta, does not. tgb beta, purified by high-pressure liquid chromatography, will not induce formation of large colonies of cells in soft agar in the absence of tgf alpha or egf. however, the combined action o ... | 1982 | 6290046 |
| molecular cloning and chromosomal mapping of a human locus related to the transforming gene of moloney murine sarcoma virus. | human dna was analyzed for the presence of sequences homologous to the transforming gene (v-mos) of moloney murine sarcoma virus. a single 2.5-kilobase pair (kbp) ecori-generated fragment of human dna was identified by using cloned v-mos as probe. this dna was molecularly cloned in a bacteriophage vector. by heteroduplex and restriction enzyme analyses, this human dna fragment, designated c-mos (human), contained a 0.65-kbp region of continuous homology with v-mos and was present as a single cop ... | 1982 | 6291031 |
| loss and restoration of glucagon receptors and responsiveness in a transformed kidney cell line. | a kidney cell line (mdck) retains an adenylate cyclase system sensitive to glucagon, vasopressin, isoproterenol and prostaglandin e1. the stimulatory effect of glucagon on camp production was selectively lost in a cloned line derived from mdck cells transformed by harvey murine sarcoma virus. sensitivity to glucagon was largely restored by treatment of the transformed cells with prostaglandin e1 or butyrate. loss and reappearance of glucagon receptors seemed to be responsible for the observation ... | 1982 | 6291963 |
| sarcoma growth factor and other transforming peptides produced by human cells: interactions with membrane receptors. | transforming growth factors (tgfs) stimulate cells to divide in monolayer cultures and to form colonies that grow progressively in soft agar. tgfs are a family of polypeptide hormones that, in vitro, confer on fibroblasts and epithelial cells properties associated with the transformed phenotype. they have been isolated from the supernatant fluids of several human and animal carcinoma and sarcoma cells. tgfs interact with epidermal growth factor (egf) membrane receptors. they are not detectable i ... | 1982 | 6292003 |