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the effect of two types of diet on populations of ascaris suum and oesophagostomum dentatum in experimentally infected pigs.this investigation compared the effect of two common types of diet on worm burdens and faecal egg counts of pigs inoculated with ascaris suum and oesophagostomum dentatum. the diets were both considered nutritionally sufficient for pigs of this age, and gave high and comparable growth rates. pigs were inoculated with both worm species at 14 weeks of age and slaughtered 3 or 12 weeks later. at 3 weeks, a. suum larvae were numerous in the small intestine of pigs fed ground barley plus protein supp ...19957567107
actions of neurotoxins (bungarotoxins, neosurugatoxin and lophotoxins) on insect and nematode nicotinic acetylcholine receptors.neurotoxins of natural origin have proved to be of considerable value in the isolation and characterization of vertebrate muscle and neuronal nicotinic acetylcholine receptors (nachrs). to date, they have been used less extensively in studies of invertebrate nachrs. here we examine how a variety of neurotoxins (the snake toxins alpha-bungarotoxin, alpha-bgt, and kappa-bungarotoxin, kappa-bgt, the molluscan toxin, neosurugatoxin, and the soft coral toxins, lophotoxin and bipinnatin-b) can be used ...19957570627
survival of salmonellas and ascaris suum eggs in a thermophilic biogas plant.in a continuous biogas plant, receiving manure from 200 dairy cows and 400 calves and young stock, survival of salmonellas and ascaris suum eggs was studied. the bacteria and parasite eggs were kept in filter sacs in the manure that had a temperature of 55 degrees c. no viable salmonellas or ascaris suum eggs could be found after 24h in the digester. survival of salmonellas and ascaris suum eggs was also studied in the manure pit where the manure was stored after digestion. the temperature in th ...19957572460
effect of allergen provocation on inflammatory cell profile and endothelin-like immunoreactivity in guinea-pig airways.the effect of allergen challenge on the number of leucocytes and the concentration of endothelin 1-like immunoreactivity (et-li) in bronchoalveolar lavage fluid (balf) was investigated in guinea-pigs sensitized to ascaris suum. the animals were twice exposed to allergen aerosol. all animals responded to the second challenge with bronchoconstriction. twelve hours later, a significant increase in the number of eosinophilic granulocytes in balf, compared to unsensitized and unprovoked control anima ...19957573819
a ph-dependent allosteric transition in ascaris suum phosphofructokinase distinct from that observed with fructose 2,6-bisphosphate.ascaris suum phosphofructokinase exhibits dramatic shifts in its circular dichroic spectra in the ph range 6 to 8. these shifts are quite distinct from those induced by the activators amp and fructose 2,6-bisphosphate. concomitant with these ph-induced spectral shifts, the enzyme also displays changes in its allosteric behavior. inorganic ions such as k+, nh+4, so4(2-), and po4(3-) also cause cd-spectral shifts similar to those produced by a change in ph. based on the evidence derived from gel f ...19957574715
humoral defense of the nematode ascaris suum: antibacterial, bacteriolytic and agglutinating activities in the body fluid.three humoral defense activities (antibacterial, bacteriolytic and agglutinating) were detected in the body fluid of the nematode ascaris suum. gram-positive bacteria (staphylococcus aureus and bacillus subtilis) were more sensitive to the antibacterial activity than the gram-negative bacteria (escherichia coli). the antibacterial activity was heat stable and was lost by trypsin digestion. the molecular mass of the factor responsible for antibacterial activity was estimated as 6 kda. the bacteri ...19957580807
role of airway eosinophils in the development of allergen-induced airway hyperresponsiveness in dogs.the role of the eosinophil in the development of allergen-induced airway hyperresponsiveness is uncertain. we examined whether the development of airway hyperresponsiveness in 17 dogs after inhalation of ascaris suum allergen (10(-6) to 10(-2) weight/volume [w/v]) was associated with increases in the number and level of activation of eosinophils before and after allergen inhalation. airway responsiveness to inhaled acetylcholine was measured before and 24 h after ascaris inhalation. eosinophil n ...19957582285
in ascaris sperm pseudopods, msp fibers move proximally at a constant rate regardless of the forward rate of cellular translocation.computer-assisted methods have been employed to obtain a high resolution description of pseudopod expansion, cellular translocation, and the subcellular dynamics of msp fiber complexes in the motile sperm of the nematode ascaris suum. although ascaris sperm translocating in a straight line or along a curved path do not retract their pseudopod or significantly alter pseudopod shape, they move in a cyclic fashion, with an average period between velocity peaks of 0.35 +/- 0.05 min, which is indepen ...19957585993
allergen-induced late-phase airways obstruction in the pig: the role of endogenous cortisol.in developing a novel model for studies of the allergen-induced late-phase airways obstruction, by using the pig, the importance of endogenous cortisol levels was examined by the use of metyrapone, a cortisol-synthesis inhibitor. specific-pathogen free pigs were actively sensitized with ascaris suum allergen. one group of pigs was treated with a constant infusion of metyrapone in order to maintain low levels of plasma cortisol. ascaris suum allergen was nebulized into the lower airways and plasm ...19957589379
allergen-induced late-phase airways obstruction in the pig: mediator release and eosinophil recruitment.the aim of this study was to develop a novel model for studies of mediator mechanisms involved in the late asthmatic reaction in the lower airways, by using the sensitized pig. the release of histamine and cysteinyl-containing leukotrienes (cys-lts), as well as the levels of inflammatory cells in blood and bronchoalveolar lavage fluid, were determined and their relationship to plasma cortisol levels and pulmonary airways obstruction was noted. specific-pathogen free pigs were actively sensitized ...19957589393
initiation of chemical studies on the immunoreactive glycolipids of adult ascaris suum.there is a general lack of basic information concerning one class of glycoconjugate, the glycolipids, from parasitic nematodes. as the prototype, the neutral glycolipid fraction derived from adult males of ascaris suum was investigated as to its chromatographic, differential chemical staining, antigenic and chemical properties. the thin-layer chromatography-resolved neutral fraction glycolipids could be classified into components of fast and slow migrating band groups. immunoreactivity was restr ...19957596644
histochemical mapping of nadph diaphorase in the nervous system of the parasitic nematode ascaris suum.nadph diaphorase has recently been discovered to be responsible for neuronal nitric oxide (no) synthase activity in mammals. it thus serves as a histochemical marker for the localization of no synthase in the nervous system. the histochemical technique was used to map out potential no-producing neurones in the nervous system of the parasitic nematode, ascaris suum. positive staining for nadph diaphorase was present in various parts of the central nervous system, in particular within selective ce ...19957596645
mast cell tryptase: a new target for therapeutic intervention in asthma. 19957613193
comparative study and cdna cloning of the flavoprotein subunit of mitochondrial complex ii (succinate-ubiquinone oxidoreductase: fumarate reductase) from the dog heartworm, dirofilaria immitis.mitochondrial complex ii functions as a fumarate reductase (frd), the reverse reaction of succinate dehydrogenase (sdh), and plays an important role in the anaerobic respiratory chain of parasitic helminths. in this study, complex ii from the dog heartworm, dirofilaria immitis adult, which is thought to act as a homolactatic fermenter, was examined in terms of its enzymatic features and primary structure in order to investigate the possible role of mitochondria in this filaria. mitochondria from ...19957613771
effects of an antibody to interleukin-5 in a monkey model of asthma.to investigate the role of interleukin-5 (il-5) on airway hyperreactivity and pulmonary inflammation in nonhuman primate airways, the effect of a neutralizing monoclonal antibody to murine il-5 (trfk-5) was investigated in a cynomolgus monkey model of allergic asthma. anesthetized ascaris-sensitive monkeys underwent bronchoalveolar lavage (bal) to assess the granulocyte content of this fluid before and 24 h after aerosolized ascaris suum extract inhalation. airway reactivity was assessed by the ...19957633694
isolation and partial characterization of eosinophil granule proteins in rats--eosinophil cationic protein and major basic protein.peritoneal eosinophilia was induced in rats using ascaris suum extract as an antigen, and characteristics of granule proteins of eosinophils collected from the peritoneal cavity were investigated. peritoneal eosinophilia was induced by injection of the antigen solution into the peritoneal cavity of the immunized rats that had been orally administered with cyclophosphamide. peritoneal cells were collected 48 h after injection of the antigen solution, incubated in plastic dishes, and nonadherent c ...19957647580
eight novel fmrfamide-like neuropeptides isolated from the nematode ascaris suum.eight fmrfamide-like neuropeptides were isolated from an extract of heads and tails from the nematode ascaris suum using seven steps of hplc. the peptides ranged in size from 8 to 14 amino acid residues: af3 (ala-val-pro-gly-val-leu-arg-phe-amide), af4 (gly-asp-val-pro-gly-val-leu-arg-phe-amide), af5 (ser-gly-lys-pro-thr-phe-ile-arg-phe-amide), af7 (ala-gly-pro-arg-phe-ile-arg-phe-amide), af9 (gly-leu-gly-pro-arg-pro-leu-arg-phe-amide), af10 (gly-phe-gly-asp-glu-met-ser-met-pro-gly-val-leu-arg-p ...19957651904
cross-reactions of sera from toxascaris leonina and ascaris suum infected mice with toxocara canis, toxascaris leonina and ascaris suum antigens.the elisa method using larval es products and homogenized toxocara canis, toxascaris leonina and ascaris suum adult worms extract, was used to determine the possible cross-reactions in balb/c and c57bl/10 mice inoculated with embryonated eggs or adult worms extract of t. leonina of t. leonina or a. suum in single and multiple doses. when we used sera of mice infected with embryonated eggs of t. leonina against different heterologous ag, no cross-reactions against t. canis es and a. suum es ag we ...19957657459
alteration in ca2+ availability involved in antigen-induced airway hyperresponsiveness in rats.the origin of ca2+ contributing to the enhancement of acetylcholine-induced bronchial smooth muscle constriction in airway hyperresponsiveness induced by antigen challenge was investigated. under ca(2+)-free (concomitant with 10(-6) m nicardipine) conditions, the contractile responses of bronchial rings to 1 mm acetylcholine were significantly greater in rings from rats with hyperresponsive airways (0.15 +/- 0.04 g) than those of rings from normal rats (0.02 +/- 0.004 g; p < 0.05). the cumulativ ...19957664816
levamisole-activated single-channel currents from muscle of the nematode parasite ascaris suum.1. the patch-clamp technique was used to examine levamisole-activated channels in muscle vesicles from ascaris suum. cell-attached and isolated inside-out patches were used. 2. levamisole (1-90 microm), applied to the extracellular surface, activated channels which had apparent mean open-times in the range 0.80-2.85 ms and linear i/v relationships with conductances in the range 19-46 ps. ion-replacement experiments showed the channels to be cation selective. 3. the kinetics of the channels were ...19937679027
a patch-clamp study of the ionic selectivity of the large conductance, ca-activated chloride channel in muscle vesicles prepared from ascaris suum.plasma membrane vesicles prepared from the bag region of the somatic muscle cell of the parasite ascaris suum contain a large conductance, voltage-sensitive, calcium-activated chloride channel. the ability of this channel to conduct a variety of anions has been investigated using the patch-clamp technique on isolated inside-out patches of muscle membrane. symmetrical cl solutions (140 mm) produced single-channel i/v plots with reversal potentials of 0 mv, substitution of bath cl by 140 mm no3, b ...19937680073
the aba-1 allergen of the nematode ascaris suum: epitope stability, mass spectrometry, and n-terminal sequence comparison with its homologue in toxocara canis.aba-1 is a major allergen of nematode parasites of the genus ascaris which includes the large roundworms of humans and pigs, a. lumbricoides and a. suum, respectively. the allergen was purified from a. suum by immunoaffinity chromatography for immunochemical examination. the ige antibody repertoire is under mhc control in infected rodents and the ige-binding epitopes were robust to treatment with heat or periodate, and electroblotting on nitrocellulose. this implies that the ige epitopes compris ...19937682160
immunocytochemical demonstration of neuropeptides in the central nervous system of the roundworm, ascaris suum (nematoda: ascaroidea).the localization and distribution of neuropeptides in the central nervous system of the pig roundworm, ascaris suum, have been determined by an indirect immunofluorescence technique in conjunction with confocal microscopy. antisera to 25 vertebrate peptides and two invertebrate peptides were used to screen the worm for immunoreactivity (ir). immunostaining was obtained with antisera to pancreatic polypeptide (pp), peptide yy (pyy), neuropeptide y (npy), gastrin, cholecystokinin (cck), substance ...19937683785
inhibition of antigen-induced airway and cutaneous responses by heparin: a pharmacodynamic study.we have previously shown that heparin attenuates the acute bronchoconstrictor response and immediate cutaneous reaction (icr) to antigen in allergic sheep. in the present investigation, we studied the pharmacodynamics of the antiallergic action of heparin. specific lung resistance (srl) was measured in eight sheep, allergic to ascaris suum antigen, before and 5 min after inhalation challenge with the antigen. on different experiment days, antigen challenge was repeated after pretreatment with 1) ...19937685753
characterization of cdna clones for the 2-methyl branched-chain enoyl-coa reductase. an enzyme involved in branched-chain fatty acid synthesis in anaerobic mitochondria of the parasitic nematode ascaris suum.the 2-methyl branched-chain enoyl-coa reductase plays a pivotal role in the reversal of beta-oxidation operating in anaerobic mitochondria of the parasitic nematode ascaris suum. an affinity-purified polyclonal anti-serum against the reductase was used to screen a cdna library constructed in lambda gt11 with poly(a)+ rna from adult a. suum muscle. a 1.2-kilobase partial cdna clone was isolated, subcloned, and sequenced in both directions. additional sequence at the 5' end of the mrna was determi ...19937693666
cyproheptadine-induced attenuation of type-i immediate-hypersensitivity reactions of airway smooth muscle from immune-sensitized cats.we assessed the effect of serotonergic inhibition by cyproheptadine on the responsiveness of tracheal smooth muscle (tsm) strips and epithelium-intact third-generation bronchial rings from immune-sensitized (ascaris suum) cats after exposure to antigen. cats were sensitized by im administration of antigen and adjuvant twice over a 4-week period. sensitization was confirmed in vivo by skin testing with antigen and by physiologic airway responses after exposure to nebulized antigen. tissues were t ...19957695137
isolation and characterization of 30-nm protein particles present in pseudopods of the amoeboid sperm of ascaris suum.particles, 30 nm in diameter, were found in triton x-100-extracted pseudopodial remnants of spermatozoa of ascaris suum. sds-page analysis of particles isolated by differential centrifugation and detergent extraction indicated that they are composed of proteins with molecular weights of 33,000 d, 42,000 d and a group between 52,000 and 56,000 d. antibodies against particle-associated proteins, used in immunoblots, showed that they are sperm-specific in ascaris, and that all of the particle-assoc ...19957702606
effect of papaya latex against ascaris suum in naturally infected pigs.an experiment was carried out to investigate the anthelmintic activity of papaya latex (carica papaya) against natural infection of ascaris suum in pigs. sixteen naturally infected pigs were, on the basis of faecal egg counts and body weight, allocated into four groups, each of four pigs. three groups (groups b, c, and d) were given papaya latex per os at dose levels of 2, 4, and 8 g of papaya latex per kg body weight, respectively. the fourth group (group a) served as a non-treated control. res ...19947706684
studies on the isolation and characterisation of a reaginic antibody in a cat.a reaginic antibody has been demonstrated, by passive cutaneous anaphylaxis (pca), in the serum of a cat infected with the microfilariae of brugia pahangi. recipient cats and pigs were challenged with an extract of ascaris suum after either a four-hour or a 72-hour period of sensitisation. when the serum was heat treated at 56 degrees c it lost its pca activity. gel filtration of the serum revealed a pattern of positive pca fractions similar to that observed in other species. attempts to purify ...19957709065
cardiovascular and hormonal responses to anaphylactic shock in the pig.cardiovascular and hormonal responses to anaphylactic shock were evaluated in anaesthetized pigs sensitized by natural exposure to ascaris suum as verified by antibodies. in six animals with such antibodies, ascaris antigen injection produced a plasma histamine increase of 52 (42-196) fold (median and range; p < 0.05), while four pigs without such antibodies served as controls with only insignificant increases in histamine. in the anaphylactic group, two of the animals died during the investigat ...19957712695
isolation and preliminary biological characterization of kpnfirfamide, a novel fmrfamide-related peptide from the free-living nematode, panagrellus redivivus.a novel fmrfamide-related heptapeptide, lys-pro-asn-phe-ile-arg-phe-nh2 (kpnfirfamide), was isolated and characterized from acid ethanol extracts of the free-living nematode, panagrellus redivivus. whole-worm extracts contained > or = 9 pmol kpnfirfamide/g wet weight. a synthetic replicate of this peptide induced a rapid relaxation of tone and inhibited spontaneous contractility in isolated innervated and denervated body-wall muscle strips of the parasitic nematode, ascaris suum. kpnfirfamide (0 ...19957716079
[survival of ascaris suum eggs in sewage treatment plant sludge].in the period between june 1992 and may 1993 the survival of nonembryonated eggs of a. suum was studied in two sludge drying beds of sewage treatment plants (stp) under different climatic-geografical conditions: stp michalovce in the east slovak lowland (elevation 111 m above sea-level) and stp poprad in the submontane area of the poprad valley (elevation 695 m). sludge drying beds of both sewage treatment plants (stp) showed different survival of eggs (fig. 1). in stp michalovce we detected a r ...19957716865
the effects of the nematode peptide, kheylrfamide (af2), on the somatic musculature of the parasitic nematode ascaris suum.af2 is an endogenous rfamide-like peptide from the parasitic nematode ascaris suum. the potent stimulatory effects of this peptide on the somatic musculature of ascaris strongly suggest that it may have an important role in the motornervous system. here we have investigated the possibility that af2 may elicit a stimulatory action on ascaris muscle by potentiating the actions of the excitatory cholinergic motonervous system either pre-synaptically, post-synaptically or both. in in vitro pharmacol ...19957724243
allergen-induced oxygen radical release from bronchoalveolar lavage cells and airway hyperresponsiveness in dogs.allergen inhalation causes airway hyperresponsiveness and airway inflammation in dogs. the purpose of this study was to determine whether allergen-induced airway hyperresponsiveness is associated with increases in oxygen radical production from bronchoalveolar lavage (bal) cells. a group of 10 random-source dogs were studied twice, 4 wk apart. on each occasion, acetylcholine (ach) airway responsiveness was measured before and 24 h after inhalation of ascaris suum or its diluent, followed by bal. ...19957735610
sequence comparison between the flavoprotein subunit of the fumarate reductase (complex ii) of the anaerobic parasitic nematode, ascaris suum and the succinate dehydrogenase of the aerobic, free-living nematode, caenorhabditis elegans.complex ii in adult mitochondria of the parasitic nematode, ascaris suum, exhibits high fumarate reductase activity and plays a key role in the anaerobic electron-transport observed in these organelles. in the present study, cdnas for the flavoprotein (fp) subunits of complex ii have been isolated, cloned and sequenced from both a. suum and the aerobic, free-living nematode, caenorhabditis elegans. additional sequence at the 3' end of the mrnas was determined by the rapid amplification of cdna e ...19947739664
the prevalence of isospora suis and strongyloides ransomi in suckling piglets in the netherlands.faecal samples from suckling piglets from 113 litters on 25 farms in the netherlands were examined to study the prevalence of gastrointestinal parasites, in particular strongyloides ransomi and isospora suis. s. ransomi was not found but i. suis was demonstrated in 17 of the 25 farms and in 41 out of 77 litters of these farms (53%). no other parasites were found. on the basis of these results, a longitudinal study on the incidence and significance of i. suis was performed on 10 farms (5 litters/ ...19947740744
immunization with ascaris suum extract impairs t cell functions in mice.the effect of an ascaris suum extract (asc) on several t cell functions was studied in mice immunized with asc and ovalbumin (oa) in complete freund's adjuvant. delayed-type hypersensitivity reactions following challenge with aggregated oa were markedly diminished in mice injected with oa plus a high dose of asc compared to oa-immunized animals. proliferation and il-2, ifn-gamma, il-4, and il-10 production in oa-stimulated lymph node cells from the oa + asc-immunized group were also inhibited. t ...19957743547
alterations in polyamine levels of nematode, earthworm, leech and planarian during regeneration, temperature and osmotic stresses.free-living nematodes, caenorhabditis elegans and dorylaimus fodori, contain putrescine and spermidine. putrescine, spermidine and spermine occur in the parasitic nematoda, ascaris suum, anisakis simplex and dirofilaria immitis. earthworms, eisenia foetida, tubifex hattai and pheretima communissima and the leech, hirudo nipponia (belonging to annelida) and the planarian, dugesia japonica (belonging to platyhelminthes) contain homospermidine and spermine in addition to putrescine and spermidine. ...19957749639
the effect of the nematode peptides sdpnflrfamide (pf1) and sadpnflrfamide (pf2) on synaptic transmission in the parasitic nematode ascaris suum.the action of two peptides isolated from the nematode panagrellus redivivus, pf1 (sdpnflrfamide) and pf2 (sadpnflrfamide) have been studied on synaptic transmission in the motornervous system of the parasitic nematode ascaris suum. intracellular recordings were made from ascaris somatic muscle cells and excitatory junction potentials (ejps) elicited by stimulation of the ventral nerve cord. the ejps were cholinergic as they were blocked by the ascaris nicotinic receptor antagonist, benzoquinoniu ...19957753584
the aba-1 allergen of the parasitic nematode ascaris suum: fatty acid and retinoid binding function and structural characterization.we report here on the structure and function of the aba-1 allergen protein of the parasitic nematode ascaris, the first nematode allergen to be characterized in detail. using the fluorescent fatty acid analog 11-(((5-(dimethylamino)-1-naphthalenyl)sulfonyl)amino)undecanoic acid (dauda), it was demonstrated that aba-1 is a fatty acid binding protein (fabp) with a high affinity for the fluorescent analog (8.8 x 10(-8) m) and for oleic acid in competition experiments (1.3 x 10(-8) m), with a single ...19957756301
the interaction among granulocyte lipid mediators and the generation of oxygen radicals in antigen-induced airway hyperresponsiveness. 19947771329
glycerophosphorylcholine, a component of both ascaris suum muscle and caenorhabditis elegans.studies of the muscle phospholipid metabolism of ascaris suum suggest an effect of cholinergic drugs on the turnover of phosphatidylcholine and the generation of glycerophosphorylcholine (gpc). 31p-nuclear magnetic resonance (nmr) studies of helminths revealed the presence of a major peak that was assigned to gpc. the primary effect of the cholinergic drugs on the parasites' phosphate profile appeared to be on the level of gpc. in in vivo studies, decreases in internal gpc concentrations occurre ...19957776117
genetic structure of midwestern ascaris suum populations: a comparison of isoenzyme and rapd markers.isoenzyme and random amplified polymorphic dna (rapd) markers were used to characterize the genetics of geographic variation among population samples of ascaris suum from midwestern localities. independent estimates of fixation indices (fst) based on isoenzyme and rapd markers showed the same general patterns of differentiation and substantial statistical correlation (r = 0.70). of the total estimated gene diversity, 9.4% (isoenzyme) and 9.2% (rapd) was distributed among infrapopulations. geogra ...19957776123
novel arylaminopyridazine-gaba receptor antagonists examined electrophysiologically in ascaris suum.the structure-activity relationships of 35 novel derivatives of 2-(carboxypropyl)-3-amino-4-methyl-6-phenyl pyridazine (sr 95103) were examined as gamma-aminobutyric acid (gaba) antagonists in the flap preparation of the parasitic nematode, ascaris suum, using a two-microelectrode current-clamp technique. all but one of the potent antagonists displaced gaba dose-response curves to the right without reduction in the maximum response. the dissociation constants of the more potent competitive antag ...19957781700
devitalization of bacterial and parasitic germs in sewage sludge during aerobic digestion under laboratory conditions.the survival of 8 bacterial species (pseudomonas sp., salmonella sp., enterobacteriae, streptococcus sp., escherichia coli) was detected in municipal sewage sludge up to 37 hours of mesophilic aerobic digestion under laboratory conditions. the model strain enterococcus faecium ccm 4231 survived almost twice as long as the above-mentioned isolates. similar findings, regarding the viability of the microorganisms studied, were also determined during thermophilic aerobic digestion of municipal sewag ...19957793018
acid-base catalytic mechanism and ph dependence of fructose 2,6-bisphosphate activation of the ascaris suum phosphofructokinase.a form of phosphofructokinase (pfk) from ascaris suum desensitized to hysteresis in the reaction time course and atp allosteric inhibition has been used to study the activation by fructose 2,6-bisphosphate (f26p2) at varied ph in both reaction directions. in the direction of phosphorylation of f6p, v and v/kmgatp are constant over the ph range 6-9, while v/kf6p decreases at low ph, giving a pk value of 7.0, and at high ph, giving a pk of 8.9. v and v/kmgatp are insensitive to the presence of f26 ...19957794888
dictyocaulus viviparus: nucleotide sequence of dv3-14. 19957797366
ige regulation by nematodes: the body fluid of ascaris contains a b-cell mitogen.nematode infection of human beings or laboratory animals leads to markedly increased levels of circulating ige, most of which is not specific to worm antigens. this phenomenon is known to be interleukin-4-dependent, but little is known about the mechanism of activation of the response. in an attempt to elucidate this mechanism, we have used a reductive approach with worm products rather than infections. in a previous article we showed that injection of the body fluid of the nematode ascaris yiel ...19957797793
immunorestorative effect of glucan immunomodulator on guinea pigs with experimental ascariosis.the immunorestorative effect of glucan immunomodulator, combined with porcine immunoglobulin and zinc (gi) on t- and b-lymphocytes and peritoneal macrophage phagocytic ability was studied in guinea pigs with experimental ascariosis (ascaris suum) after a cyclophosphamide (cy)-evoked immunosuppression. during the migration phase of a. suum infection gi exerted a significant restorative effect on the cy-reduced percentage occurrence of t- and b-cell populations in the mesenteric, mediastinal and h ...19947810068
persistent airway hyperresponsiveness and histologic alterations after chronic antigen challenge in cats.we studied the effect of chronic immune sensitization on the airway reactivity and associated cytologic and histologic alterations in initially nonatopic cats, a species that spontaneously develops idiopathic asthma. seven cats were sensitized by intramuscular injection of ascaris suum antigen (aa) for 4 wk, and four other cats served as sham controls. airway sensitization was demonstrated by an increased response to nebulized aa in sensitized animals (rl = 45.9 +/- 6.1 cm h2o/l/s, versus a base ...19957812551
the ca-activated chloride channel of ascaris suum conducts volatile fatty acids produced by anaerobic respiration: a patch-clamp study.plasma membrane vesicles prepared from the bag region of the somatic muscle cell of the parasite ascaris suum contain a large conductance, voltage-sensitive, calcium-activated chloride channel. the ability of this channel to conduct a variety of carboxylic acids, a number of which are products of anaerobic respiration, was investigated using the patch-clamp technique and isolated inside-out patches of muscle membrane. the channel has a conductance of 140 ps in symmetrical 140 mm chloride. replac ...19947815451
[study of the efficacy of sewage treatment systems for fluid waste on pig farms in slovakia].bacteriological, helminthological and physico-chemical examinations were carried out to investigate the technological procedure in three water treatment plants treating slurry from large-capacity pig farms (kosická polianka, spisské vlachy, vel'ký dur). our investigations were focused on the testing of effectiveness of mechanical, chemical and biological treatment system most frequently used in slovakia. our investigations revealed that water-treatment plants, operating on pig farms, fulfil only ...19947817500
ascaris suum: characterization of transmural and hypodermal potentials.electrophysiological measurements demonstrate that an electrochemical potential exists across the body wall of parasitic nematodes. the ionic dependence of this transmural electrical potential (etm) in the gastrointestinal nematode ascaris suum was investigated using conventional electrophysiological techniques. etm recorded from intact a. suum maintained in artificial pseudocoelomic fluid (apf) was -40 +/- 12 mv. this potential was more sensitive to external acetate than to na+, k+, or cl-, alt ...19957821415
metabolism of af1 (knefirf-nh2) in the nematode ascaris suum. 19947821552
stage-specific isoforms of complex ii (succinate-ubiquinone oxidoreductase) in mitochondria from the parasitic nematode, ascaris suum.complex ii from mitochondria of the adult parasitic nematode, ascaris suum, exhibits high fumarate reductase activity and plays a key role in the anaerobic electron transport observed in these organelles. in contrast, mitochondria isolated from free living second stage larvae (l2) of a. suum show much lower fumarate reductase activity than those from adults, whereas succinate dehydrogenase activities of mitochondria in both stages are comparable. in the present study, biochemical and antigenic p ...19957822332
peptides related to the diploptera punctata allatostatins in nonarthropod invertebrates: an immunocytochemical survey.the allatostatins are a family of peptides isolated originally from the cockroach, diploptera punctata. related peptides have been identified in periplaneta americana and the blowfly, calliphora vomitoria. these peptides have been shown to be potent inhibitors of juvenile hormone synthesis in these species. a peptide inhibitor of juvenile hormone biosynthesis has also been isolated from the moth, manduca sexta; however, this peptide has no structural homology with the d. punctata-type allatostat ...19947822491
identification and analysis of a cuticular collagen-encoding gene from the plant-parasitic nematode meloidogyne incognita.the vast majority of proteins in the nematode cuticle are collagens. cuticular collagen-encoding genes (col) have been described for the animal parasites ascaris suum and haemonchus contortus and for the free-living caenorhabditis elegans. the proteins encoded by all these genes seem to have the same basic structure, indicating that there is a conserved subfamily of cuticular col in these nematodes. in this paper, we describe the identification and characterization of a cdna (lemmi 5) which corr ...19947828882
effect of an inhaled corticosteroid on airway eosinophils and allergen-induced airway hyperresponsiveness in dogs.the presence of airway eosinophils before allergen inhalation may contribute to the development of allergen-induced airway responses. we examined whether a reduction in airway eosinophil numbers before allergen inhalation as a result of inhalation of the corticosteroid budesonide would prevent allergen-induced airway hyperresponsiveness in seven dogs. acetylcholine airway responsiveness was measured before and 24 h after inhalation of ascaris suum allergen (10(-6)-10(-2) wt/vol) or its diluent o ...19947836134
localization of diploptera punctata allatostatin-like immunoreactivity in helminths: an immunocytochemical study.the nervous systems of helminths are predominantly peptidergic in nature, although it is likely that the full range of regulatory peptides used by these organisms has yet to be elucidated. attempts to identify novel helminth neuropeptides are being made using immunocytochemistry with antisera raised against peptides isolated originally from insects. one of these antisera was raised against allatostatin iii, a peptide isolated originally from the cockroach, diploptera punctata, and a member of a ...19957845717
pyruvate dehydrogenase complexes from the equine nematode, parascaris equorum, and the canine cestode, dipylidium caninum, helminths exhibiting anaerobic mitochondrial metabolism.the pyruvate dehydrogenase complex (pdc) has been purified to apparent homogeneity from 2 parasitic helminths exhibiting anaerobic mitochondrial metabolism, the equine nematode, parascaris equorum, and the canine cestode, dipylidium caninum. the p. equorum pdc yielded 7 major bands when separated by sds-page. the bands of 72, 55-53.5, 41 and 36 kda corresponded to e2, e3, e1 alpha and e1 beta, respectively. the complex also contained additional unidentified proteins of 43 and 45 kda. incubation ...19947870133
supramolecular assemblies of the ascaris suum major sperm protein (msp) associated with amoeboid cell motility.sperm of the nematode, ascaris suum, are amoeboid cells that do not require actin or myosin to crawl over solid substrata. in these cells, the role usually played by actin has been taken over by major sperm protein (msp), which assembles into filaments that pack the sperm pseudopod. these msp filaments are organized into multi-filament arrays called fiber complexes that flow centripetally from the leading edge of the pseudopod to the cell body in a pattern that is intimately associated with moti ...19947876359
metal ion activator effects on intrinsic isotope effects for hydride transfer from decarboxylation in the reaction catalyzed by the nad-malic enzyme from ascaris suum.the mechanism of the oxidative decarboxylation reaction catalyzed by the nad-malic enzyme from ascaris suum has been examined with several different divalent metal ion activators and dinucleotide substrates. primary deuterium and tritium isotope effects have been obtained and, in combination with the partitioning ratios of the oxalacetate intermediate to malate and pyruvate, have been used to calculate commitment factors, intrinsic deuterium isotope effects on the hydride transfer step, and intr ...19957880820
obtention of ovine ige from heterohybridoma.mesenteric and bronchial lymph node cells from sheep immunized with ascaris suum antigens in combination with bordetella pertussis vaccine were fused with mouse myeloma cell lines, p3-x63-ag8.653, nso.u and ns1.1.ag1.1. one heterohybridoma cell line (ns1.1.ag1.1 x sheep) producing ovine immunoglobulin e was detected by the passive cutaneous anaphylaxis test.19947889039
morphometric estimation of superoxide generation in allergen-induced airway hyperresponsiveness.we previously reported that the airway hyperresponsiveness (ahr) that follows antigen challenge is mediated, in part, by the generation of reactive oxygen species. the purpose of this study was to provide quantitative morphologic evidence of oxygen radical production in the airways during antigen-induced ahr.19957898054
use of specific antisera for the localisation and quantitation of leucokinin immunoreactivity in the nematode, ascaris suum.1. the leucokinins (lks) are a group of eight related peptides isolated from the cockroach, leucophaea maderae. 2. antisera raised against lk-v, which were specific for the conserved mid to c-terminal region of the lks, were used to immunostain the parasitic nematode ascaris suum. 3. lk-ir was observed in neurons in the anterior nerve ring, retrovesicular ganglion, and ventral and dorsal nerve cords of the parasite. immunostaining was specific in that it was abolished by preabsorption of the ant ...19937904924
immunocytochemical demonstration of peptidergic and serotoninergic components in the enteric nervous system of the roundworm, ascaris suum (nematoda, ascaroidea).the localization and distribution of neuropeptides and an indoleamine (serotonin or 5-hydroxytryptamine) in the enteric nervous system (ens) of the pig roundworm, ascaris suum, have been determined by the application of an indirect immunofluorescence technique in conjunction with confocal scanning laser microscopy. whole-mount preparations of pharyngeal, intestinal and rectal regions were screened with antisera to 23 vertebrate peptides, 2 invertebrate peptides and serotonin (= 5-ht). positive i ...19947908737
the molecular structure of the complex of ascaris chymotrypsin/elastase inhibitor with porcine elastase.the intestinal parasitic worm, ascaris suum, produces a variety of protein inhibitors that defend the organism against the host's proteinases. eight different proteins from ascaris suum have been identified as inhibitors of serine proteinases, targeting chymotrypsin, elastase and trypsin. these inhibitors share 30-40% sequence identity with one another, but have virtually no sequence identity with members of any of the other families of serine proteinase inhibitors.19947922044
engineering ascaris hemoglobin oxygen affinity in sperm whale myoglobin: role of tyrosine b10.the contribution to oxygen stabilization of a tyrosine residue in topological position (b10) has been studied in sperm whale myoglobin by simultaneous replacement of residues at positions (b10), (e7) and (e10) as suggested by analysis of the sequence of high oxygen affinity hemoglobins, such as that of the nematode ascaris suum. kinetic and equilibrium experiments with the gaseous ligands oxygen and carbon monoxide show that indeed the introduction of tyrosine (b10), together with replacement of ...19947925944
the motile major sperm protein (msp) of ascaris suum forms filaments constructed from two helical subfilaments.the amoeboid motility nematode sperm is mediated by cytoskeletal filaments composed of major sperm protein (msp). we have used electron microscopy and image processing to show that msp filaments are constructed from two subfilament strands which are themselves formed from a helical arrangement of subunits. the subfilaments are based on left-handed helices of pitch 9 nm that then coil along right-handed helical tracks of pitch 22.5 nm to form filaments. the subfilaments appear to be indistinguish ...19947932742
characterization of the n-acetyltransferases respectively responsible for arylalkylamine and diamine acetylation in ascaris suum. 19947935612
the role of dityrosine formation in the crosslinking of cut-2, the product of a second cuticlin gene of caenorhabditis elegans.a second cuticlin gene, cut-2, of the nematode caenorhabditis elegans, has been isolated and its genomic and cdna sequences determined. the gene codes for a component of cuticlin, the insoluble residue of nematode cuticles. conceptual translation of cut-2 reveals a 231-amino acid secreted protein which, like cut-1, begins with a putative signal peptide of 16 residues. the central part of the protein consists of 13 repetitions of a short hydrophobic motif, which is often degenerated with substitu ...19947935621
nucleotide correlations that suggest tertiary interactions in the tv-replacement loop-containing mitochondrial trnas of the nematodes, caenorhabditis elegans and ascaris suum.in the predicted secondary structures of 20 of the 22 trnas encoded in mitochondrial dna (mtdna) molecules of the nematodes, caenorhabditis elegans and ascaris suum, the t psi c arm and variable loop are replaced with a loop of 6 to 12 nucleotides: the tv-replacement loop. from considerations of patterns of nucleotide correlations in the central regions of these trnas, it seems highly likely that tertiary interactions occur within five sets of binary and ternary combinations of nucleotides that ...19947937159
allergen-induced changes in bone marrow progenitors and airway responsiveness in dogs and the effect of inhaled budesonide on these parameters.airway inflammation is implicated in the pathogenesis of the airway hyperresponsiveness in asthma. an increased production of inflammatory cell progenitors may contribute to asthmatic airway inflammation. although the number of circulating inflammatory cell progenitors in asthmatic subjects increases after allergen inhalation, no direct evidence exists for increased bone marrow progenitor production. we examined the effect of allergen inhalation on bone marrow progenitor production in seven dogs ...19947946389
copper-zinc superoxide dismutase from ascaris suum (nematoda): purification and characterization.copper-zinc superoxide dismutase from ascaris suum (nematoda) was purified in a new, more efficient, and faster manner. the process included differential centrifugation, fractionation with ammonium sulfate, and sodium dodecyl sulfate-polyacrylamide electrophoresis, yielding a 340-fold purification (specific activity of 47 units/mg). optimal storage conditions, optimal ph range, thermostability, molecular weight and ultraviolet-visible absorption spectrum of the enzyme are described, and a new en ...19947948773
2-methylbutyryl-coa: succinate acyl-coa transferase activity and function in ascaris suum muscle.a branched-chain acyl-coa transferase activity which transfers coenzyme a from either 2-methylbutyryl or 2-methylvaleryl-coa to succinate is present in the muscle mitochondria from the intestinal nematode, ascaris suum. its physiological function is discussed. this activity appears to differ from the previously described acetyl-coa: propionate and propionyl-coa:succinate acyl-coa transferases on the basis of heat stability, substrate specificity and the requirement of a "factor" from boiled asca ...19947953070
the fmrfamide-like neuropeptide af2 (ascaris suum) is present in the free-living nematode, panagrellus redivivus (nematoda, rhabditida).available primary structural information suggests that the fmrfamide-related peptides (farps) from parasitic and free-living nematodes are different, and that free-living forms may not represent appropriate models for the study of the neurochemistry of parasitic forms in the laboratory. however, here we report the isolation and unequivocal identification of af2 (originally isolated from the parasite, ascaris suum) from acidified alcoholic extracts of the free-living species, panagrellus redivivu ...19947970891
the effect of immunization of pigs with ascaris suum cuticle components on the development of resistance to parenteral migration during a challenge infection.the development of immunity to ascaris suum was studied in pigs immunized with isolated cuticle fragments from a. suum second and third stage larvae (l2/l3) and adult worms, and compared with other methods that stimulate a strong protective response in pigs. a significant protective response was seen in animals immunized with isolated cuticle fragments from a. suum l2/l3 and adults, but it was less than that seen in animals inoculated with uv-irradiated eggs or naturally exposed to eggs on a dir ...19947975188
sevoflurane and isoflurane protect against bronchospasm in dogs.halothane and isoflurane have been shown to be effective in reversing bronchoconstriction; however, the effects of sevoflurane have not been well defined. we studied whether sevoflurane, compared with isoflurane, attenuates bronchospasm in dogs.19947978482
structural characterization of an ascaris myoglobin.globin was purified from the body wall of adults of the parasitic nematode ascaris suum. internal peptide fragments were sequenced and cdnas encoding a polypeptide of 154 amino acids isolated by polymerase chain reaction. the polypeptide lacks a signal sequence, identifying it as a cytosolic myoglobin-like species. the native protein is a dimer. the predicted amino acid sequence shares several unusual substitutions with other nematode globins. like the abundant pseudocoelomic a. suum hemoglobin ...19947982924
novel regulation of pyruvate dehydrogenase phosphatase purified from anaerobic muscle mitochondria of the adult parasitic nematode, ascaris suum.pyruvate dehydrogenase (pdhb) phosphatase has been purified to apparent homogeneity from mitochondria of the adult parasitic nematode, ascaris suum. the enzyme is a heterodimer of 89 and 50 kda, as judged by sds-polyacrylamide gel electrophoresis. it appeared to copurify with its substrate, pyruvate dehydrogenase (e1) and could be separated by chromatography on superose 12 in the presence of 0.5 m nacl. phosphatase activity was absolutely dependent on mg2+, with an apparent km of about 4 mm. in ...19947989326
antigen-induced airway hyperresponsiveness is associated with airway tissue nep hypoactivity in rats.in the present study, the role of sensory neuropeptides in the airway hyperresponsiveness (ahr) was investigated. first, the effect of the depletion of sensory neuropeptides by systemic capsaicin treatment on the ahr to acetylcholine (ach) induced by repeated antigenic challenge to sensitized rats was studied. we secondly investigated whether the neutral endopeptidase (nep) activity was altered at the antigen-induced ahr. male wistar rats were sensitized and repeatedly challenged with dnp-ascari ...19947990652
leukotriene d4 receptor antagonism reduces airway hyperresponsiveness in monkeys.airway hyperresponsiveness (ahr) and pulmonary inflammation are observations that are consistently associated with asthma and also occur in a well-characterized monkey model of asthma. the following study was performed to determine whether treatment with an ltd4 receptor antagonist, ici 198,615, could attenuate antigen-induced pulmonary inflammation and ahr in monkeys using the following protocol. on day 0, the pc200 (the concentration of methacholine (mch) that doubled respiratory system resist ...19948003852
the development and evolution of actin-containing organelles during spermiogenesis of a primitive nematode.spermatogenesis in the primitive marine nematode sphaerolaimus hirsutus (chromadoria, sphaerolaimidae) was investigated by examining the ultrastructure and cytochemistry. spermatozoa are lenticular cells of about 15 microns in diameter and are devoid of flagellum and acrosome as in other nematodes. in spermatocytes, dictyosomes produced transient structures, the fibrous body-membranous organelle complexes (fb-mo). in spermatids, the fbs were arranged as cartwheel spokes, with the fbs in the cent ...19938004009
the detection of akh/hrth-like peptides in ascaridia galli and ascaris suum using an insect hyperglycaemic bioassay.evidence for the presence of adipokinetic hormone/hypertrehalosaemic hormone (akh/hrth)-like peptides in the parasitic nematodes ascaridia galli and ascaris suum has been obtained using insect bioassays which measure hyperglycaemic responses to peptides belonging to the akh/hrth family of insect hormones. a peptide fraction extracted from heads and tails of ascaridia galli evoked a dose-dependent hyperglycaemic response when injected into the cockroach, periplaneta americana. maximal bioactivity ...19948008462
regulation of the ascaris major sperm protein (msp) cytoskeleton by intracellular ph.the development and locomotion of the amoeboid sperm of the nematode, ascaris suum, depend on precise control of the assembly of their unique major sperm protein (msp) filament system. we used fluorescence ratio imaging of cells loaded with bcecf to show that intracellular ph (phi) is involved in controlling msp polymerization in vivo. spermatogenesis is marked by a cycle of msp assembly-disassembly-reassembly that coincides with changes in phi. in spermatocytes, which contain msp in paracrystal ...19948020106
the epidemiology of ascaris suum infections in pigs in northern ireland, 1969-1991.this paper presents the results of analysis of condemnations of pig livers due to cirrhosis which, in temperature climates, gives an accurate estimation of ascaris suum infections. over the 23 years analysed, a very highly significant upward trend in the prevalence of a. suum is demonstrated. the association between the prevalence and the mean air temperature over the early summer period is highlighted. thorough cleansing of pig units in early spring and at various times during the summer are su ...19948025848
respiratory chain of the lung fluke paragonimus westermani: facultative anaerobic mitochondria.the respiratory chain of adult paragonimus westermani, a lung fluke, was characterized in isolated mitochondria. the fluke mitochondria exhibited cyanide- and antimycin a-sensitive succinate oxidase activity at a rate of 16.8 nmol o2 min-1 mg-1 protein. the succinate oxidation was shown to be stimulated by adp and linked to the formation of membrane potential. the specific activities of oxidoreductases composing the succinate oxidase system, i.e., succinate-ubiquinone and succinate--cytochrome c ...19948031121
hepatic vascular response to anaphylaxis in isolated canine liver.we determined the vascular response to anaphylaxis in isolated canine livers perfused with autologous blood at a constant pressure via the portal vein, with hepatic artery ligation to simplify the vascular system. we also studied the validity of the double occlusion pressure (pdo) as a measure of the capillary pressure (pc) in the isolated canine liver. pdo was compared with pc measured using the traditional isogravimetric method (pc,i), and both parameters showed a strong correlation (pdo = 0.3 ...19948048632
ultrastructural localization of pancreatic polypeptide- and fmrfamide immunoreactivities within the central nervous system of the nematode, ascaris suum (nematoda: ascaroidea).a post-embedding immunogold technique has been used to examine the subcellular distribution of immunoreactivities to vertebrate pancreatic polypeptide (pp) and to the invertebrate peptide, fmrfamide within the central nervous system (cns) of the nematode, ascaris suum. gold labelling of peptide was localized exclusively over dense-cored vesicles within nerve cell bodies, nerve axons and nerve terminals of the main ganglia and nerve cords in the cns. double-labelling of peptides demonstrated an a ...19948052514
biophysical model of the transcuticular excretion of organic acids, cuticle ph and buffer capacity in gastrointestinal nematodes.a biophysical model was developed, using ascaris suum as a model gastrointestinal nematode, to provide quantitative perspectives into the microenvironmental ph within the water-filled, porous, negatively charged cuticle matrix of gastrointestinal nematodes. the central features of the model include (a) the constant rate of excretion of organic acid metabolites across the cuticle, (b) the relationship between cuticle ph and pka of the organic acids that determines the fraction of unionized and io ...19948069580
molecular genealogy of some nematode taxa as based on cytochrome c and globin amino acid sequences.we have begun to reconstruct the ancient history of the nematode phylum based on cytochrome c and globin amino acid sequences. the data suggest that the nematode ancestor diverged from a line leading to mammals about 1 billion years ago and that the most recent common ancestor of the extant species caenorhabditis elegans, trichostrongylus colubriformis, nippostrongylus brasiliensis, ascaris suum, and pseudoterranova decipiens lived about 550 my ago. the rhabditids and strongylids emerged as one ...19948075836
[ascaridiasis zoonoses: visceral larva migrans syndromes].the syndrome of visceral larva migrans is a zoonotic disease due to the migration in human of nematodes larval, specially ascarid. since the larvae fail to complete their migrating cycle in humans, the diagnosis of toxocariasis infection remains only serologic. we have been able to demonstrate by the technique of agar diffusion and the western-blotting method that the etiology due to toxocara canis was twice as much frequent as the one due to toxocara cati in the syndrome of visceral and ocular ...19948076198
primary and higher order structures of nematode (ascaris suum) mitochondrial trnas lacking either the t or d stem.by fractionation using polyacrylamide gel electrophoresis and/or a preparative hybrid selection method employing solid-phase dna probes, we prepared and characterized mitochondrial trnas from the body wall muscle of ascaris suum, all of which are thought to lack either the t stem or the d stem from their gene sequences (okimoto, r., and wolstenholme, d. r. (1990) embo j. 10, 3405-3411). some of the partially purified trnas were appreciably aminoacylated with an extract of a. suum mitochondria. t ...19948077242
characterization and biological activities of anti-brugia pahangi tubulin monoclonal antibodies.three monoclonal antibodies (mab) specific to beta-tubulin were used to investigate the heterogeneity of tubulins from nematodes and mammals. western blot analysis of one-dimensional sds-page showed that anti-brugia pahangi tubulin mab 1b6 and p3d react with epitope(s) specific to nematode beta-tubulin and recognize tubulin from adults and microfilariae of b. pahangi, adult b. malayi and dirofilaria immitis, eggs of haemonchus contortus and adult ascaris suum. however, the same mab did not recog ...19938106183
[alpha-amylase release by pancreatic acini isolated from guinea pigs infected with invasive eggs of ascaris suum].the studies were carried out on dispersed acini prepared from pancreas of guinea pigs infected with 5000 invasive eggs of ascaris suum. the basal amylase secretion and that stimulated with acetylcholine and carbachol or blocked with atropine was determined. the basal secretion of amylase was significantly higher in pancreatic acini isolated from infected animals. the stimulation by acetylcholine and carbachol of these acini was weaker than in the control group. these results suggest that choline ...19938122421
[in vitro study of anthelmintic influence on activities of digestive enzymes in extracts from the gut of ascaris suum and pig's pancreas].the effect of eight anthelmintics (rintal, fenbesan, telmin, banminth, pyrantel, nilverm, levamisol and bioscardina) on the alpha- and gamma-amylases, trypsin, and lipase from pig's pancreas and gut of ascaris suum was determined. in extracts from a. suum gut also the maltase, trehalase and saccharose activities were examined. all drugs tested did not influence on the host's alpha-amylase. telmin and bioscardina were inhibitors of gamma-amylase, rintal and telmin--of trypsin, fenbesan and biosca ...19938128727
use of body fluid of adult female ascaris suum as an antigen in the enzyme-linked immunosorbent assay (elisa) for diagnosis of swine ascariosis.the enzyme-linked immunosorbent assay (elisa) was used to detect ascaris suum antibodies in swine sera with adult body fluid (abf) as an antigen. the assay was standardized with respect to the antigen concentration and serum and conjugate dilutions. cross reaction was found between the antigen and the sera from the swine infected with metastrongylus apri. the elisa was more sensitive than the complement fixation test. five protein peaks were obtained from abf by gel filtration on sephacryle s-30 ...19938132972
bactericidal activity in the pig roundworm ascaris suum.a potent, humoral, bactericidal activity against micrococcus luteus was discovered in pseudocoelomic fluid of the pig roundworm, ascaris suum. the activity, which was not bacteriolytic, was not due to lysozyme or to a dietary antibiotic. it was not inactivated by exposure to 100 degrees c, to low or high ph, or to ethanol. dialysis, electrophoresis and agar-diffusion experiments suggested that the main antibacterial activity in the fluid was associated with a basic substance of molecular weight ...19948144403
effect of antigen on the glycoconjugate profile of tracheal secretions and the epithelial glycocalyx in allergic sheep.to characterize the glycoconjugate composition of tracheal secretions and the apical glycocalyx of the tracheal epithelium under baseline conditions and after antigen challenge, sheep allergic to ascaris suum were intubated with a double-balloon nasotracheal tube to create a tracheal chamber. after an initial tracheal lavage, the animals were either exposed to intratracheally nebulized phosphate-buffered saline (pbs) (3 ml, n = 6) or a. suum antigen (251,000 protein nitrogen units in 3 ml of pbs ...19948151061
characterization of identifiable neurones in the head ganglia of the parasitic nematode ascaris suum: a comparison with central neurones of caenorhabditis elegans.intracellular recordings have been made from neurones in the head ganglia of ascaris. the neurones had low resting membrane potentials of -21 +/- 9 mv (n = 78) and a relatively high input resistance (e.g. 25 m omega for a 100 microns cell). in all cases the intracellular location of the recording electrode was verified by injection of the fluorescent marker, 5,6-carboxyfluorescein (cbxf). to ascertain whether or not the low membrane potential was due to impalement damage, the same neurone was re ...19948152859
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