Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| purified trypanosoma cruzi specific glycoprotein for discriminative serological diagnosis of south american trypanosomiasis (chagas' disease). | 1990 | 1726799 | |
| functional and antigenic properties of the major cysteine proteinase (gp57/51) of trypanosoma cruzi. | 1990 | 1726800 | |
| autoantibodies in chagas' heart disease: possible markers of severe chagas' heart complaint. | 1990 | 1726801 | |
| [experimental antitriatomic program in santiago]. | the rural migration to urban centers occurred since the 40s in many latin american countries, including santiago the capital city of chile, originated a growing belt of premises built with light poor material (the rests of previous rural habitations, mud, pieces of timber, plastic and cardboard for walls, and cane stalks and artificial clinkstones for roofs) giving raise to many types of slums. this situation facilitated the passive transport of the different instars, including eggs, of triatomi ... | 1991 | 1726919 |
| lethality of triatomines (hemiptera: reduviidae), vectors of chagas' disease, feeding on blood baits containing synthetic insecticides, under laboratory conditions. | a laboratory study was conducted to test the toxicity of synthetic insecticides added to defibrinated sheep blood kept at room temperature and offered as food to the following triatomine species: triatoma infestans, panstrongylus megistus, triatoma vitticeps, triatoma pseudomaculata, triatoma brasiliensis and rhodnius prolixus. the insecticides used, at a concentration of 1 g/l, were: hch, ddt, malathion and trichlorfon, and the lethalithy observed at the end of a 7-day period varied according t ... | 1991 | 1726952 |
| coculture of human peripheral blood mononuclear cells with trypanosoma cruzi leads to proliferation of lymphocytes and cytokine production. | trypanosoma cruzi, which causes chagas' disease, has been shown to cause polyclonal proliferation of lymphocytes after infection in vivo. this paper demonstrates that coculture of human pbmc with t. cruzi cl strain leads to proliferation of lymphocytes, which peaks on days 5 to 7 after infection. approximately 15% of lymphocytes in culture undergo blast transformation. the proliferation of lymphoblasts can be measured by [3h]tdr incorporation, because the parasites incorporate little tdr. parasi ... | 1992 | 1727869 |
| trypanosoma cruzi: predominance of igg2a in nonspecific humoral response during experimental chagas' disease. | the kinetic and isotypic pattern of hypergammaglobulinemia has been investigated in c3h/hej infected with trypanosoma cruzi. hypergammaglobulinemia appeared 14 days postinfection, increased until day 28 postinfection, and persisted throughout the chronic phase (greater than 60 days of infection). the main isotype secreted was igg2a, reaching 10-fold the control level. high titers of autoantibodies were found of igm and igg subclasses. isotypic characterization of antibodies against myosin, myeli ... | 1992 | 1730271 |
| polyclonal cell activity of a repeat peptide derived from the sequence of an 85-kilodalton surface protein of trypanosoma cruzi trypomastigotes. | some in vitro and in vivo biological activities of an octadecapeptide derived from an 85-kda surface protein of trypanosoma cruzi trypomastigote were studied. the peptide coupled to a carrier protein induced the proliferative response of lymph node cells from mice immunized with various antigens. moreover, sera from mice immunized with the coupled peptide were found to contain antibodies against a number of self and nonself antigens: fibronectin, bovine serum albumin, myosin, tetanus toxoid, ova ... | 1992 | 1730508 |
| interleukin 10 and interferon gamma regulation of experimental trypanosoma cruzi infection. | studies were undertaken to determine whether interleukin 10, (il-10) a cytokine shown to inhibit interferon gamma (ifn-gamma) production, was involved in trypanosoma cruzi infections in mice. exogenous ifn-gamma protects mice from fatal infection with t. cruzi. furthermore, resistant b6d2 mice developed fatal t. cruzi infections when treated with neutralizing anti-ifn-gamma monoclonal antibody (mab). thus, endogenous as well as exogenous ifn-gamma is important in mediating resistance to this par ... | 1992 | 1730915 |
| anti-cd4 abrogates rejection and reestablishes long-term tolerance to syngeneic newborn hearts grafted in mice chronically infected with trypanosoma cruzi. | the contribution of autoimmunity in the genesis of chronic chagas' heart pathology is not clear. in the present study, we show that: (a) balb/c mice chronically infected with trypanosoma cruzi reject syngeneic newborn hearts; (b) in vivo treatment with anti-cd4 but not anti-cd8 monoclonal antibodies (mabs) abrogates rejection; (c) cd4+ t cells from chronically infected mice proliferate in vitro to syngeneic myocardium antigens and induce heart graft destruction when injected in situ; (d) anti-cd ... | 1992 | 1730921 |
| trypanosoma cruzi trans-sialidase and neuraminidase activities can be mediated by the same enzymes. | trans-sialidase and neuraminidase activities have been detected on the surface membrane of trypomastigotes of trypanosoma cruzi, and both have been implicated in the parasite's invasion of host cells. we show here that these enzymes are structurally related. they are recognized by two independently derived monoclonal antibodies, are anchored to the membrane by glycosylphosphatidylinositol, copurify by ion exchange, molecular sieving, and hydrophobic chromatography, have maximal activities betwee ... | 1992 | 1732417 |
| trypanosoma cruzi: stage expression of calmodulin-binding proteins. | the subcellular distribution of calmodulin-binding proteins in three life stages of trypanosoma cruzi was analyzed by a [125i]calmodulin gel overlay procedure under conditions where proteolysis was kept to a minimum. it was found that t. cruzi contains a complex profile of calcium-dependent calmodulin-binding proteins and that several of these polypeptides were differentially expressed at specific stages of development. the majority of these stage-specific polypeptides was found in the particula ... | 1992 | 1740174 |
| experimental american leishmaniasis and chagas' disease in the brazilian squirrel monkey: effect of dual infection on antibodies to parasite antigens. | adult, laboratory-bred squirrel monkeys (saimiri sciureus) were infected with either leishmania braziliensis braziliensis or l. b. panamensis and, 42 weeks later, they were challenge-infected with trypanosoma cruzi. another group of monkeys was infected with t. cruzi and challenged with l. b. braziliensis after 42 weeks. immunoblotting was used to examine parasite antigens bound by antibodies in plasma obtained from the monkeys during the course of primary and challenge infections. during primar ... | 1991 | 1743848 |
| control of chagas disease. report of a who expert committee. | 1991 | 1746164 | |
| trypanosoma cruzi induces suppression of dna synthesis and inhibits expression of interleukin-2 receptors by stimulated human b lymphocytes. | trypanosoma cruzi, the causative agent of chagas' disease, suppresses immune responses during the acute phase and has been shown to induce multiple cellular alterations in activated human t lymphocytes. however, no information is available regarding the effects of this parasite on human b cells. using an in vitro culture system, in which purified t. cruzi are co-cultured with either peripheral blood mononuclear cells (pbmc) or b-cell-enriched preparations (bce), we studied whether the organism c ... | 1991 | 1748480 |
| complete structure of the glycan of lipopeptidophosphoglycan from trypanosoma cruzi epimastigotes. | the lipopeptidophosphoglycan is the major cell surface glycoconjugate of the epimastigote forms of the parasitic protozoan trypanosoma cruzi. a detailed partial structure for this molecule has been reported (previato, j. o., gorin, p. a. j., mazurek, m., xavier, m. t., fournet, b., wieruszesk, j. m., and mendonca-previato, l. (1990) j. biol. chem. 265, 2518-2526). in this study, we complete the primary structure assignments and describe the microheterogeneity found in the lipopeptidophosphoglyca ... | 1991 | 1748644 |
| reactivation of chagas' myocarditis during therapy of hodgkin's disease. | a 46-year-old female patient with chronic chagas' myocarditis without heart failure was submitted to chemotherapy because of hodgkin's disease. during treatment the frequency of polymorphic ventricular extrasystoles raised and the patient died by a sudden cardiac arrest. autopsy revealed chronic chagas myocarditis with an acute exacerbation. nests of amastygotes with a density of up to 14 pseudocysts/cm2 were found in the myocardium, although restricted to the septum and left ventricular postero ... | 2015 | 1750119 |
| characterization of host cell-derived membrane proteins of the vacuole surrounding different intracellular forms of trypanosoma cruzi in j774 cells. evidence for phagocyte receptor sorting during the early stages of parasite entry. | trypanosoma cruzi, an obligate intracellular protozoan parasite, exhibits developmental regulation of virulence. although both noninfective epimastigote and infective trypomastigote stages of t. cruzi enter phagocytic cells via the formation of a parasitophorous vacuole (pv), only the latter developmental stages survive ingestion and perpetuate the infection. to determine whether the membrane composition of pv surrounding these different stages might contribute to differences in the outcome of i ... | 1991 | 1753102 |
| prevalence of antibody to trypanosoma cruzi among blood donors in los angeles, california. | transfusion-associated chagas' disease is a serious public health problem in central and south america. with the recent influx of immigrants from chagas' disease-endemic areas, concern about the risk of disease from blood transfusion has increased in the united states. to assess the prevalence of trypanosoma cruzi infection in one area, 1024 consecutive blood donations from 988 voluntary blood donors at a medical center in los angeles county were screened serologically. the median age of donors ... | 1991 | 1755086 |
| mimicry in trypanosoma cruzi: fantasy and reality. | chronic infection of mammals by trypanosoma cruzi often results in severe autoimmune and inflammatory pathology. extensive antigen cross-reactivity between the parasite and its mammalian hosts has also been reported. these findings have stimulated speculation that trypanosoma cruzi uses antigenic mimicry as a mechanism for escaping the host immune system. this may not be the case and the observed antigen cross-reactivity may be a result of perturbations of the immune system such that common, nor ... | 1991 | 1755976 |
| an 85-kilodalton surface antigen gene family of trypanosoma cruzi encodes polypeptides homologous to bacterial neuraminidases. | we have determined the sequence of a cdna (tt34c1) encoding a trypanosoma cruzi trypomastigote stage-specific 85-kda surface glycoprotein (gp85). within the peptide sequence of tt34c1 are two 8-amino acid motifs, ser-x-asp-x-gly-x-thr-trp, that are characteristic of bacterial neuraminidases. analysis of the tt34c1 sequence predicts the presence of an amino-terminal signal sequence and a hydrophobic carboxy-terminus that is probably replaced by a glycosyl phosphatidylinositol membrane anchor. gp8 ... | 1991 | 1762630 |
| [axonal polyneuropathy in chagas disease]. | we report the case of a 44-year-old woman presenting with chronic symmetrical, sensitive polyneuropathy on the lower limbs in the course of chagas' disease. the electrophysiological findings were in keeping with axonal degeneration. the histological data displayed axonal degeneration with perivascular inflammatory mononuclear cells in the epineurium, with some neutrophils and eosinophils. mononuclear cells surrounding endoneurial vessels were observed. laboratory data did not suggest neither a m ... | 1991 | 1763261 |
| acute fatal trypanosoma cruzi meningoencephalitis in a human immunodeficiency virus-positive hemophiliac patient. | a 37-year-old hemophiliac patient with known, asymptomatic human immunodeficiency virus infection and chronic chagas' disease was admitted to the hospital complaining of fever and headache. a computed tomographic scan revealed multiple ring-enhancing lesions in both cerebral hemispheres. no antibodies to trypanosoma cruzi were found in the cerebrospinal fluid. treatment for toxoplasmosis of the central nervous system, which was considered the most likely diagnosis, was instituted, but the patien ... | 1991 | 1763799 |
| [evaluation of interleukin-2 soluble receptor in patients with trypanosomiasis americana in panama]. | the quantitative determination of interleukin-2 receptor (sil-2r) in the serum of patients with acute or chronic chagas disease is compared with values found in normal individuals. the mean value of soluble il-2r in patients with acute chagas' disease was found to be 3,282 +/- 171 u/ml. the mean value of sil-2r in serum samples from chronic chagasic patients was 511 +/- 207 u/ml, while in the control or "normal" group of persons the mean value for sil-2r was 366 +/- 108 u/ml. in patients with ea ... | 1991 | 1767036 |
| lipoamide dehydrogenase from trypanosoma cruzi: some properties and cellular localization. | lipoamide dehydrogenase (e.c. 1.6.4.3) was found in trypanosoma cruzi, tulahuen strain, stocks tul-2 and q501, and ca-1 strain. after differential centrifugation of epimastigote homogenates, ammonium sulfate fractionation of the 105,000 g supernatant yielded a partially purified preparation which precipitated between 0.40 and 0.80 ammonium sulfate saturation. the enzyme (a) catalyzed the oxidation of dihydrolipoamide by nad+ and the reduction of lipoamide by nadh, the forward reaction being 2.5- ... | 1991 | 1768255 |
| toxicity of sera from individuals with chagas' disease to cultured rat embryos: role of antibodies to laminin. | in previous studies antilaminin antibodies in the sera of immunized monkeys and rats were found to be toxic to cultured rat embryos. in order to extend these studies to humans, head-fold stage rat embryos were cultured for 48 hours on ten different serum samples from individuals with chagas' disease. all embryos (n = 20) cultured on these sera were found to be abnormal. using elisa, western immunoblot, and indirect immunofluorescence it could be shown that antibodies in these sera reacted with l ... | 1991 | 1771597 |
| [left ventricular mechanics in chagas' disease and primary dilated myocardiopathies. a hemodynamic study]. | the evolution of several mechanical parameters of left ventricular function was studied in 32 control subjects, 171 chronic chagasic and 60 primary dilated cardiomyopathy patients, which were submitted to an extensive invasive and non-invasive protocol. preload and afterload (end-diastolic and end-systolic stress), contractile status (ejection fraction), the magnitude (mass/index) and adequacy of hypertrophy (mass/volume ratio) and afterload (systolic stress/volume ratio) were derived from the i ... | 2007 | 1772315 |
| changes in polypeptide expression following trypanosoma cruzi differentiation from trypomastigotes to amastigotes. | changes in the dynamic of expression of polypeptides following the differentiation from infective trypomastigotes to multiplicative amastigote forms of trypanosoma cruzi were mapped by two-dimensional gel electrophoresis and quantitatively analyzed by laser densitometry. following the differentiation from trypomastigotes to amastigotes the expression of the polypeptides 212, 183, 176, 149, 50-55, 43, 39, 34 and 28 kda is turned off in multiplicative amastigotes, whereas the expression of the pol ... | 1991 | 1772436 |
| assay for detection of trypanosoma cruzi antibodies in human sera based on reaction with synthetic peptides. | synthetic peptides modelled according to the amino acid sequences derived from the repeated domains of five trypanosoma cruzi antigens were used in an immunoradiometric assay to detect antibodies appearing after natural human infections. an enzyme-linked immunosorbent assay and an indirect immunofluorescence assay performed with a complex antigenic mixture from parasites were used as controls. the results indicate that the synthetic peptides were recognized by a large proportion of serum samples ... | 1991 | 1774331 |
| new enzyme immunoassays for specific assay and general detection of trypanosoma cruzi, epimastigotes. | a highly specific competitive enzyme-linked immunosorbent assay for the epimastigote of tulahuen strain was developed by using the usual 3 immunological reagents, a rabbit antiserum specific for t. cruzi, epimastigote of tulahuen strain, beta-d-galactosidase-labeled goat anti-rabbit immunoglobulin g and the solid-phase cell fragments of the epimastigote of tulahuen strain. a new method, the selected antibody enzyme immunoassay (saeia) which generally detected all strains of the epimastigote test ... | 1991 | 1775098 |
| degradation of oxidised insulin a and b chains by the major cysteine proteinase (cruzipain) from trypanosoma cruzi epimastigotes. | 1991 | 1775177 | |
| subcellular localization of glutamate dehydrogenases and alanine aminotransferase in epimastigotes of trypanosoma cruzi. | the subcellular localization of nad- and nadp-linked glutamate dehydrogenases (gdh-nad and gdh-nadp), alanine aminotransferase (alat) and aspartate aminotransferase (asat) in epimastigotes of trypanosoma cruzi was studied by digitonin extraction from whole cells, subcellular fractionation by differential centrifugation and isopycnic ultracentrifugation. all enzymes presented both a cytosolic and a mitochondrial form; in addition, gdh-nadp seems to have a third, still undefined, localization. the ... | 1991 | 1778428 |
| lactation reduces mortality but not parasitaemia during the acute phase of trypanosoma cruzi infection in mice. | 1991 | 1780986 | |
| accumulation of brown adipose tissue in patients with chagas heart disease. | brown adipose tissue (bat) is an important source of non-shivering thermogenesis. increased bat amounts have been reported to occur in association with several diseases, including congestive heart failure. the objective of the present study was to determine whether bat accumulation occurs in patients with chagas disease. histological sections of peri-adrenal tissue obtained at autopsy from 259 patients were examined. of these patients, 58 had the digestive form of chagas disease, 50 had the card ... | 2007 | 1780987 |
| trypanosoma cruzi: incorporation of [3h]-palmitic acid and [3h]-galactose into components shed by trypomastigotes. | trypomastigotes were metabolically labeled with [3h]-palmitic acid or [3h]-galactose and labeled components were detected in the culture medium. thin layer chromatography of the shed material showed several lipids in the [3h]-palmitic acid labeled sample while the sugar was mainly incorporated into macromolecules. the material incorporated with the lipidic precursor was fractionated by deae-sephadex (acetate form) and the amount of radioactivity was ten times higher in the acidic lipids than in ... | 1991 | 1781795 |
| [entomological surveillance of chagas' disease in the state of sao paulo]. | drastic modifications in agrarian space, with the reductions of primary forests, along with changes in rural production systems have led to the growth of salaried employment and also caused rural workers to move to the peripheral areas of cities. there has also been an intense and continuous intervention undertaken by the superintendência de controle de endemias (sucen) through the use of pesticides in the fight against triatomine bugs in houses and peridomestic areas of the state of s. paulo. a ... | 1991 | 1784957 |
| synthesis of n-benzyloxycarbonyl-l-cysteinylglycine 3-dimethylaminopropylamide disulfide: a cheap and convenient new assay for trypanothione reductase. | trypanothione disulfide [n1,n8-bis(glutathionyl)-spermidine], the physiological substrate for the chemotherapeutic target enzyme trypanothione reductase, is difficult to isolate, expensive to buy, and awkward to synthesize. here we describe the straightforward synthesis of n,n'-bis(benzyloxycarbonyl)-l-cysteinylglycyl-3-dimethylaminopropylam ide disulfide, which is shown to be a good alternative substrate for the trypanothione reductases from crithidia fasciculata and trypanosoma cruzi. | 1991 | 1789428 |
| pathologic features of dogs inoculated with north american trypanosoma cruzi isolates. | twenty-three clinically normal beagles were inoculated with north american trypanosoma cruzi isolates from an opossum (tc-o), an armadillo (tc-a), or a dog (tc-d). the dogs were grouped according to the clinical outcome of inoculation. group 1 consisted of 7 dogs inoculated with tc-o or tc-a that died or were euthanatized during acute stages of disease. group 2 consisted of 5 dogs inoculated with tc-o or tc-a, that also developed acute disease, but survived to develop chronic disease. group 3 co ... | 1991 | 1789518 |
| dna fluorescence induced by polymethine cation pyrvinium binding. | pyrvinium is a polymethine cation which shows interesting fluorescence emission and dna binding properties. in diluted aqueous solution, pyrvinium pamoate induced a bright yellow fluorescence in kinetoplast dna from trypanosoma cruzi epimastigotes as well as in chicken erythrocyte nuclei under a wide range of excitations. no fading was observed after mounting in suitable media. spectroscopic studies on pyrvinium solutions revealed bathochromic and hypochromic shifts in the absorption spectrum of ... | 1991 | 1791161 |
| changes in cell-surface carbohydrates of trypanosoma cruzi during metacyclogenesis under chemically defined conditions. | highly purified lectins with specificities for receptor molecules containing sialic acid, n-acetylglucosamine (d-glcnac), n-acetylgalactosamine (d-galnac), galactose (d-gal), mannose-like residues (d-man) or l-fucose (l-fuc), were used to determine changes in cell-surface carbohydrates of the protozoal parasite trypanosoma cruzi during metacyclogenesis under chemically defined conditions. of the d-galnac-binding lectins, bs-i selectively agglutinated metacyclic trypomastigotes, mpl was selective ... | 1991 | 1791438 |
| further characterization of trypanosoma cruzi gp57/51 as the major antigen expressed by differentiating epimastigotes. | study of trypanosoma cruzi metacyclogenesis under chemically defined conditions showed that the expression of a group of acidic polypeptides with a molecular weight of 45-50 kda is markedly increased on adhesion of epimastigotes to the culture vessels. immunochemical analysis revealed that these developmentally regulated polypeptides are structurally related to, and possibly homologous with, a major t. cruzi antigen, namely, gp57/51, a glycoprotein that has recently been characterized as a cyste ... | 1991 | 1792225 |
| vector population responses to control interventions. | in 1981, kenyan authorities attempted eradication of tsetse (glossina pallidipes) from the lambwe valley, by sequential aerial spraying of endosulfan. fly populations were reduced by over 99.9% in the main habitats, but recovered to their original levels within one year. in 1986, an argentine research team attempted local elimination of domestic triatominae (triatoma infestans) in an area of santiago del estero. house infestation rates were reduced to an apparent zero, but recovered to pre-contr ... | 1991 | 1793271 |
| chagas disease control in brazil: which strategy after the attack phase? | the main target of brazilian chagas disease programme (cdp) has been the control of domestic triatomine bugs, since more than 80% of human disease is attributable to transmission by these vectors. the global cdp attack phase began in 1983, after a political and technical decision to cover all the 2,400 municipalities of the endemic area with regular insecticide spraying. at present, this vertical and almost military approach is ending, with good entomological results in nearly 70% of the whole a ... | 1991 | 1793283 |
| trypanosoma cruzi exoantigens: can those recognized by sera from chagasic patients trigger a protective immune response in mice? | exoantigens (ea) of trypanosoma cruzi released in blood during the acute phase of experimental murine infection and recognized as antigens by sera from chagasic patients were grouped into two zones: one zone of pi 4-5 (ea4-5), which had components of 35 kda, 50 kda and slightly higher than 100 kda, mw, and another zone, of pi 6-7 (ea 6-7), with ea of 50 kda, 66-80 kda and higher than 100 kda. immunization of mice with ea4-5 or ea6-7 prior to infection induced a protective immune response, as jud ... | 1991 | 1796212 |
| partial purification and some properties of a hemolymph lectin from panstrongylus megistus (hemiptera, reduviidae). | hemagglutinating activity was studied in homogenates of three embryonic stages, and in the hemolymph of most instar larvae and in adult insects of panstrongylus megistus, an important chagas' disease vector in brazil. a hemolymph lectin from the 5th instar larvae of p. megistus was purified through a biospecific adsorption by using formaldehyde-treated erythrocytes. the lectin fraction was desorbed with 0.2m d-galactose in 0.15m nacl. the lectin fraction activity was inhibited by l-rhamnose, d-l ... | 1991 | 1796814 |
| the validity of serodiagnosis using a monoclonal antibody against trypanosoma cruzi-specific mr 25,000 antigen for chagasic patients without cardiomyopathy. | 1991 | 1796873 | |
| the possible mechanism of action of igg antibodies and platelets protecting against trypanosoma cruzi infection. | 1. the role of igg antibody and platelets in the mechanism of defense against trypanosoma cruzi infection is reviewed. 2. experimental data showing the participation of the different igg subclasses in the immune lysis and immune clearance of the parasites are discussed. 3. the involvement of the platelets in the removal of the parasites from the circulation is considered. 4. it is suggested that igg anti-t. cruzi antibodies interact with circulating parasites leading to formation of microaggrega ... | 1991 | 1797263 |
| research for new antichagasic drugs. | a series of ten 1-[(5-nitrothenylidene)amino]azoles has been synthesized by the reaction of 5-nitrothiophene-2-carbaldehyde with 1-aminopyrazole, 1-aminoimidazole, 1- and 4- amino-1,2,4-triazoles, 1-aminoindole, 1- and 2-aminoindazoles, 1-aminobenzimidazole and 1- and 2-aminobenzotriazoles. physical data, spectroscopic characteristics and biological properties of all the derivatives have been examined. the antiprotozoal activity has been tested against trypanosoma cruzi, comparative to nifurtimo ... | 1991 | 1797419 |
| chagas disease in north-west argentina: infected dogs as a risk factor for the domestic transmission of trypanosoma cruzi. | the association between household seroreactivity to trypanosoma cruzi in dogs and children and t. cruzi infection rates in domestic triatoma infestans was investigated in 1988-1989 in the rural community of amamá, north-west argentina, where house spraying with residual pyrethroids was carried out in 1985. three years after spraying, a greater reduction of the average t. cruzi prevalence rate in dogs (from 83% to 40%) than in children (from 48% to 30%) was accompanied by a substantial decrease i ... | 1991 | 1801342 |
| density-dependent timing of defaecation by triatoma infestans. | defaecation timing of the triatominae vectors of trypanosoma cruzi directly affects the transmission probability of chagas disease to mammal hosts. experimental studies with fifth instar nymphs of triatoma infestans showed that defaecation time was negatively affected by blood meal size and positively affected by starvation period and bug initial weight. since blood meal size and starvation period are both density-dependent, low density domestic populations of t. infestans would represent a high ... | 1991 | 1801360 |
| a comparative pathological study of three strains of trypanosoma cruzi in an experimental model. | trypanosoma cruzi, the etiological agent of chagas' disease, shows a wide variation in its biological behaviour depending on the geographical distribution of different strains. moreover, some strains can show variations with the course of time. we have studied the tissular tropism of three strains of t. cruzi, cali, bolivia and y, from different geographical origins (colombia, bolivia and brasil respectively) on swiss mice in order to detect any possible modification in their behaviour attributa ... | 1991 | 1802119 |
| laboratory diagnosis of trypanosomiasis. | although african trypanosomiasis, or sleeping sickness, in humans was largely under control approximately 30 years ago, today most of the historical foci show an alarming increase in the number of cases, particularly those in east and central africa. this article describes the epidemiology, clinical features, and diagnostic techniques for both african and american trypanosomiasis. | 1991 | 1802527 |
| the status of transmission of trypanosoma cruzi in an endemic area of argentina prior to control attempts, 1985. | a field survey of transmission of trypanosoma cruzi was carried out in an area which had never been sprayed with insecticide. a population census, mapping and house classification, examination of insects in houses, and a human serological survey were performed. the entomological examination showed that 97% of the houses (433/445) were infested by triatoma infestans and that 31% of the bugs examined were infected with t. cruzi. the level of infection, as well as the number of insects captured, ha ... | 1991 | 1809241 |
| [esophageal achalasia: 20 years' experience with non surgical treatment]. | the purpose of this paper is to report the experience acquired in pneumatic dilatation in achalasia of the esophagus up to 1990. two hundred and six patients were studied in that period (x 50, 7 years, m/f 1:1). according to x rays the distribution was: grade i 17.4%, grade ii 54.8%, grade iii 14% and grade iv 13.5%. the associated esophageal pathology was: hiatus hernia 9.7%, esophagitis 5.8%, benign stenosis 2.4%, cancer 1.4%, ulcer and diverticula 0.9% and schatzki's ring and leiomyoma 0.4% r ... | 1991 | 1811399 |
| [immune response in trypanosoma cruzi infection]. | 1991 | 1815267 | |
| [canine visceral leishmaniasis: evaluation of the serologic method used in epidemiologic studies]. | a comparative study was made of eluates of the blood of dogs experimentally infected with different trypanosomatids. using antigens prepared from promastigotes of leishmania mexicana, l. braziliensis and l. chagasi, assessments were made by the indirect immunofluorescence test. the results showed a sensitivity of 87.5% in the diagnosis of canine visceral leishmaniasis, independent of antigen used. cross-reactions occurred in 75% of cases of cutaneous leishmaniasis and 83.3% of dogs with chagas' ... | 1991 | 1815283 |
| [xenodiagnosis and criteria to assess the level of parasitemia in chronic chagasic patients]. | xenodiagnosis sensibility and its relation to chagas' disease chronic phase parasitemia was verified in 104 patients. the nymphs of the triatomine species, simultaneously utilized in the xenodiagnosis of these patients, divided into two groups, were examined in different ways. the positivity rates achieved in the two groups (40.0% and 42.4%) and the individual positivity rates suggest a reavaliation in what is called "quantification of the chronic chagasic patient parasitemia", considering the m ... | 2006 | 1815286 |
| human infection with trypanosoma cruzi and leishmania viannia braziliensis (lvb). a clinical perspective. | 1991 | 1815292 | |
| recombinant trypanosoma cruzi antigens and chagas' disease diagnosis: analysis of a workshop. | a workshop organized by the ibero-american project of biotechnology evaluated the diagnostic potential of several cloned trypanosoma cruzi recombinant antigens for chagas' disease serodiagnosis. a set of recombinants, antigen 2, antigen 13, sapa, h49, a13, jl5, jl7, jl8, jl9, and ra1 provided by three different south american laboratories were probed with a panel of 236 south american serum samples. antigens jl7, h49, antigen 2, and a13 scored as the best diagnostic recombinant reagents. the res ... | 1991 | 1815706 |
| cytochemical localization of adenylate cyclase in the three developmental forms of trypanosoma cruzi. | we used ultrastructural cytochemistry to localize adenylate cyclase activity in the three evolutive forms of trypanosoma cruzi. we detected the enzyme by using adenylimidodiphosphate as substrate, prostaglandin e1 as enzyme stimulator, theophylline as inhibitor of adenosine 3'-5'-monophosphate phosphodiesterase, and either cerium chloride or lead nitrate as capture agent. deposition of imidodiphosphate was stronger in trypomastigotes than in amastigotes and epimastigotes. differences in the reac ... | 1991 | 1818203 |
| action of exogenous potassium and calcium ions on in vitro metacyclogenesis in trypanosoma cruzi. | the cations ca2+ and k+ and the anions cl-, hco3-, and po4- were studied for their contribution to metacyclic trypomastigote formation of trypanosoma cruzi in starvation media consisting of phosphate-buffered saline (pbs) + 10 mm proline + 10 mm sodium acetate as well as one of the following salts: 0.035% nahco3 (pbsnpa), 0.035% k2co3 (pbskpa) or 0.035% k2hpo4 (pbsppa). isolates cl and dm28c were activated to transform with 5% co2 and the percent metacyclogenesis determined after incubation for ... | 1991 | 1818206 |
| presence of amastigotes in the weber's lingual salivary gland of trypanosoma cruzi-infected mice. | the authors studied histopathologically the presence of amastigotes in the weber's lingual salivary gland of mice infected with rc strain of trypanosoma cruzi. amastigotes were found in weber's gland acini cells, excretory duct cells, intralobular connective tissue, muscle fibers and inside the acini lumen. | 1991 | 1819359 |
| use of isosorbide dinitrate for the symptomatic treatment of patients with chagas' disease achalasia. a double-blind, crossover trial. | 1. a randomized, double-blind, placebo-controlled trial was carried out to determine the efficacy of isosorbide dinitrate (isd) on dysphagia in patients with chagasic achalasia. 2. twenty-three patients with chagas' disease and dysphagia entered the study and 20 (87%) completed the two 7-day treatment periods. subjects were given either 5 mg isd (12 patients) or placebo (11 patients) by the sublingual route for the first 7 days. on the 8th day, patients crossed over and began another 7-day perio ... | 1991 | 1822997 |
| comparative study of different methods used to isolate trypanosoma cruzi from defibrinated blood of irradiated mice. | different methods are being used for the isolation and purification of trypanosoma cruzi blood forms from infected vertebrate hosts. in this study we compare four of these methods (differential centrifugation, ficoll-hypaque, histopaque 1077 and metrizamide) in terms of parasite recovery rates, contamination with cells, duration of the process and role of host irradiation. male albino swiss mice irradiated in a gamma cell 220 (500 rads) were inoculated with cl and vl-10 t. cruzi strains and bled ... | 1991 | 1823252 |
| diffusion-in-gel enzyme-linked immunosorbent assay (dig-elisa) for chagas' disease serodiagnosis. | 1. diffusion-in-gel enzyme-linked immunosorbent assay (dig-elisa) was standardized and evaluated for the diagnosis of chagas' disease, in comparison with the conventional serological tests indirect immunofluorescence (ifi), passive hemagglutination (pha) and complement fixation (cf). 2. a total of 236 serum samples positive and negative for the serodiagnosis of chagas' disease were studied. the group included 50 serum samples serologically positive for leishmaniasis and 36 positive for malaria. ... | 1991 | 1823262 |
| [possible role of autonomic heart dysfunction in sudden death associated with chagas disease]. | 1991 | 1823743 | |
| [ventricular mechanics and cardiac arrhythmias in patients with chagasic and primary dilated cardiomyopathy. echo-electrocardiographic follow-up]. | to determine the prognostic significance of several parameters of left ventricular mechanics and of complex ventricular arrhythmias. | 1991 | 1823747 |
| [why is there predominance of right heart failure in chagas' disease?]. | 1991 | 1824192 | |
| [ergospirometry as a method for predicting the clinical course of ischemic, chagas and idiopathic myocardiopathy]. | to compare and evaluate the modifications variables obtained through spyroergometry in groups of patients with ischemic cardiomyopathy (is), chagas' disease (ch), and idiopathic cardiomyopathy (id). to study tolerance to anaerobic conditions of patients from the above groups who died. | 1991 | 1824216 |
| [evaluation of syncope in patients with chronic chagas heart disease]. | to evaluate causes of syncope in patients with chagas' disease and intraventricular conduction disturbances. | 1991 | 1824525 |
| immune complex-mediated glomerulopathy in experimental chagas' disease. | to investigate the development of glomerulopathy during the chronic phase of experimental chagas' disease, c3h-hej mice were infected with trypanosoma cruzi trypomastigotes. deposits of igg, igm, and c3 in renal mesangium were observed by immunofluorescence (if) to increase in size as a function of time after infection (4-6 months). t. cruzi antigens were codeposited in glomeruli with ig and c3. electron-dense deposits were visualized in mesangial and paramesangial areas by electron microscopy. ... | 1991 | 1824567 |
| characterization of a membrane antigen of leishmania amazonensis that stimulates human immune responses. | to investigate human immune responses to defined leishmania ag we have begun to characterize biochemically and immunologically, an abundant 42-kda surface ag of leishmania amazonensis, a causative agent of human leishmaniasis. we have shown that this ag, la gp42, is expressed on the surface of l. amazonensis promastigotes, being anchored to the membrane by a glycosyl-phosphatidylinositol moiety. as demonstrated by lectin blotting studies, la gp42 is glycosylated, binding both con a and wheat ger ... | 1991 | 1824776 |
| characterization of a glycosyl-phosphatidylinositol-anchored membrane protein from trypanosoma cruzi. | four monoclonal antibodies (mabs) specific for trypanosoma cruzi were obtained. flow cytometry analysis showed that these four mabs stained the membranes of the three main morphological forms of t. cruzi: amastigotes, trypomastigotes, and epimastigotes. the four mabs seemed to recognize the same 50- to 55-kda antigen that was revealed by immunoblotting. competition experiments revealed that they defined at least two different epitopes on the molecule. the antigen was detected on the external sur ... | 1991 | 1825989 |
| a major proportion of n-glycoproteins are transiently glucosylated in the endoplasmic reticulum. | n-linked, high-mannose-type oligosaccharides lacking glucose residues may be transiently glucosylated directly from udp-glc in the endoplasmic reticulum of mammalian, plant, fungal, and protozoan cells. the products formed have been identified as n-linked glc1man5-9glcnac2 and glucosidase ii is apparently the enzyme responsible for the in vivo deglucosylation of the compounds. as newly glucosylated glycoproteins are immediately deglucosylated, it is unknown whether transient glucosylation involv ... | 1991 | 1826090 |
| immune response and trypanosoma cruzi infection in trypanosoma rangeli-immunized mice. | the capacity of trypanosoma rangeli antigens to induce immune response in mice was analyzed and the course of the infection was studied in immunized animals challenged with virulent forms of t. cruzi. balb/c mice were immunized with supernatant of disrupted epimastigotes of t. rangeli and with epimastigotes (epi) of t. rangeli fixed with glutaraldehyde. both of the antigens were emulsified with incomplete freund's adjuvant (ifradj). all of the animals received t. cruzi tulahuen antigens in the f ... | 1991 | 1828328 |
| conservation of cation-transporting atpase genes in leishmania. | dna fragments isolated from leishmania donovani atpase genes were used to analyze the organization and expression of cation transporting atpase genes in l. donovani, leishmania tropica, leishmania mexicana, leishmania braziliensis, trypanosoma brucei and trypanosoma cruzi. the atpase loci in all leishmania species contained a tandem pair of atpase genes arranged in head-to-tail orientation and separated by approximately 2 kb. no restriction site polymorphisms were detected in the internal portio ... | 1991 | 1828863 |
| rna-protein complexes mediate in vitro capping of the spliced-leader primary transcript and u-rnas in trypanosoma cruzi. | a 39-nucleotide spliced leader (sl) is joined to the 5' ends of trypanosome mrnas in a bimolecular or trans-splicing process. the sl in trypanosoma cruzi is transcribed as an approximately 110-nucleotide rna (sl-rna or sl primary transcript) bearing the 39-nucleotide sl at the 5' end. the sl-rna is 5' capped by a guanylyltransferase activity prior to trans-splicing and trypanosome mrnas thus obtain their mature caps from the sl by trans-splicing. we have previously characterized a guanylyltransf ... | 1991 | 1829524 |
| disruption of mitochondrial function as the basis of the trypanocidal effect of trifluoperazine on trypanosoma cruzi. | the tricyclic anti-calmodulin drug trifluoperazine (tfp) inhibited growth and motility of epimastigotes of trypanosoma cruzi, at concentrations lower than 100 microm, and motility and infectivity of the bloodstream trypomastigote form at 200 microm. electron microscopy of tfp-treated epimastigotes showed that the major effect was at the mitochondrial level, with gross swelling and disorganization. the oligomycin-sensitive, mitochondrial atpase was completely inhibited by 20 microm tfp, and the s ... | 1991 | 1829686 |
| cardiac levels of norepinephrine, dopamine, serotonin and histamine in chagas' disease. | extraction and measurement of biogenic amines (norepinephrine, dopamine, serotonin and histamine) were carried out on human ventricular myocardium obtained from autopsies of individuals divided in the three following groups: chronic chagas' heart disease (with congestive heart failure: 16 cases, and with sudden and unexpected death: 13 cases); hypertensive heart disease (12 cases); and control patients (with no heart disease: 12 cases). the myocardial samples were obtained from the free walls of ... | 1991 | 1831806 |
| stage-specific phospholipase c-mediated release of trypanosoma cruzi neuraminidase. | 1991 | 1833644 | |
| macrophage regulation of immune responses of spleen cells from mice infected with trypanosoma cruzi. | mice infected with the protozoan parasite, trypanosoma cruzi, are known to be immunosuppressed in responsiveness to heterologous antigens and parasite-specific antigens. this suppression is mediated by suppressor macrophages and is exemplified by deficient t cell activity and abnormal cytokine production. neither the mechanism by which suppressor macrophages effect suppression nor the characteristics of these suppressor macrophages is known. in the present study, we analyzed the regulatory cell ... | 1991 | 1834349 |
| histopathology of specialized and ordinary myocardium and nerves in chronic chagas disease, with a morphometric study of inflammation and fibrosis. | chagas disease, in the chronic phase, is known to be characterized by cardiac failure, and/or arrhythmias. to assess the involvement of the conduction system and of the working myocardium, morphometric and immunohistochemistry studies have been carried out on 4 autoptic hearts of chronic chagasic myocardiopathy. the characterization of interstitial infiltrates was performed by lymphocyte immunophenotyping with immunocytochemical techniques. these infiltrates were more prominent in the working my ... | 1991 | 1836420 |
| a calmodulin-activated (ca(2+)-mg2+)-atpase is involved in ca2+ transport by plasma membrane vesicles from trypanosoma cruzi. | high-affinity ca(2+)-activated atpases that do not show any demonstrable dependence on mg2+ have been reported in the plasma membranes of different trypanosomatids, and it has been suggested [mclaughlin (1985) mol. biochem. parasitol. 15, 189-201; ghosh, ray, sarkar & bhaduri (1990) j. biol. chem. 265, 11345-11351] that these enzymes may have a role in ca2+ transport by the plasma membrane and in the regulation of intracellular ca2+ in these parasites. in this report we investigated ca2+ transpo ... | 1991 | 1837215 |
| the complete sequence of a shed acute-phase antigen of trypanosoma cruzi. | 1991 | 1840626 | |
| characterization of a candidate gene for gp72, an insect stage-specific antigen of trypanosoma cruzi. | the trypanosoma cruzi insect stage-specific antigen gp72 was purified from epimastigotes and the amino acid sequences of peptide fragments determined. oligonucleotides derived from these data were used to amplify and clone a cdna sequence, which was used to isolate a full-length gene. all the sequenced peptides were encoded within the gene. the characteristics of the encoded 62,600-da protein, including a potential amino-terminal signal sequence, a hydrophobic carboxy-terminus, and a large numbe ... | 1991 | 1840630 |
| neurovegetative regulation and cardiovascular diseases. | heart period and arterial pressure short term variabilities contain rhythmic oscillations which might provide information on neural mechanisms regulating cardiovascular function. continuous electrocardiographic and/or arterial pressure signals, after appropriate analogue to digital conversion, furnish the time series which constitute the basis for spectral analysis of their variabilities. under stationary conditions, this methodology can be utilized to assess both total variability and the power ... | 1991 | 1840813 |
| xviii annual meeting on basic research in chagas' disease. caxambu, november 5-7, 1991. abstracts. | 1991 | 1841178 | |
| [association of encephalic vascular accidents and chagas disease]. | the frequency of strokes was studied in chronic chagasic and years of age, non-chagasic patients, older than 15 coming to necropsy in uberaba, from 1979 than 1988. the study consisted of paired sex and age matched controls. two hundred and eight pairs were analysed. either ischemic or hemorrhagic strokes were found in 41 (19.7%) of the chagasics and in 55 (26.4%) of the non-chagasic, a difference not significant at the level of 5%. twelve (75%) of the former had infarcts and 4 (25%) had brain he ... | 1991 | 1841424 |
| [prospective study of chagas disease in newborn children with placental infection caused by trypanosoma cruzi (santa cruz-bolivia)]. | in order to know the significance of placental infection by t. cruzi 820 newborn infants (nb) weighing less than or equal to 2500 grs were examined both clinically and by the strout method and histopathological sections of the placenta in order to detect congenital infection with chagas' disease. thirty five (4.26%) nb presented a placentary infections by t. cruzi, but having a negative direct parasitological examination in the cord blood, these nb were followed up parasitologically (microhemato ... | 1991 | 1841425 |
| [trypanosoma cruzi infection in hamsters]. | 1991 | 1841428 | |
| [comparison of parasitology of the central vein of the adrenal gland and the other tissues in chronic chagas disease]. | by morphological and morphometric analyses of serial sections the occurrence of t. cruzi nests in the central vein and in the parenchyma of adrenal glands, in the left ventricular wall and in the inferior vena cava wall in chronic chagasic patients was studied. of 36 cases 50% showed parasites in the adrenal central vein wall (total 29 nests), 3.1% showed parasites in the vena caval wall (only i nest) and 16.8% we found parasites in the myocardiocytes (total 23 nests). the density of parasites m ... | 2006 | 1841430 |
| an epidemiological model for the dynamics of chagas' disease. | a model for the transmission dynamics of chagas' disease is presented. the structure of the model is similar to that of the ross-macdonald model for malaria but includes an extra infectious compartment (chronically ill individuals) which is characteristic of chagas' disease. in chagas' disease there are two-main forms of transmission, by blood transfusion and by vector biting. the former is more common in urban environments and the latter is characteristic of rural settings. the characteristic l ... | 1991 | 1841638 |
| effects of azadirachtin in rhodnius prolixus: data and hypotheses. | the effects of azadirachtin a, a tetranortriterpenoid from the neem tree azadirachta indica j., on both development and interaction between trypanosoma cruzi, the causative agent of chagas' disease, and its vector rhodnius prolixus were studied. given through a blood meal, a dose-response relationship of azadirachtin was established using antifeedant effect and ecdysis inhibition as effective parameters. a single dose of azadirachtin a was able to block the onset of mitosis in the epidermis and ... | 1991 | 1841982 |
| interaction of trypanosoma cruzi with macrophages influence of temperature. | the interaction of macrophages and t. cruzi has been studied in vitro culture under three different temperatures. after 24 hours incubation at 29 degree c a large number of recognizable parasites inside macrophages is observed with evidences of cell divisions. at video microscopy they show a slow motion and a predominance of epimastigotes and some round shapes (amastigotes). this was corroborated at the light and electron microscopes. no evidence of lysis in the phagosome vacuoles was observed. ... | 1991 | 1842026 |
| trypanosoma cruzi: growth of clones on solid medium using culture and blood forms. | 1991 | 1842394 | |
| trypanosoma rangeli (tejera, 1920) isolated from a sylvatic rodent (echimys dasythrix) in santa catarina island, santa catarina state: first report of this trypanosome in southern brazil. | a trypanosome strain isolated from a sylvatic rodent (echimys dasythrix) from santa catarina island (santa catarina state, brazil) was characterized by the following methods: experimental transmission and development in invertebrate and vertebrate hosts, morphometry, cross protection, complement sensitivity, lectin agglutination and isoenzyme profiles. comparisons were made with standard trypanosoma cruzi and t. rangeli strains. all methods except isoenzyme analysis led to the identification of ... | 1991 | 1842404 |
| the importance of the opossum (didelphis albiventris) as a reservoir for trypanosoma cruzi in bambuí, minas gerais state. | in a survey realized on the sylvatic and peridomestic environments at bambuí county, minas gerais state, 44 (37.9%) out of 116 opossums (didelphis albiventris) captured were found to be naturally infected with trypanosoma cruzi. one hundred and forty three parasite samples were obtained from 43 infected opossums using simultaneously hemoculture, xenodiagnosis (triatoma infestans, panstrongylus megistus and rhodnius neglectus) and examination of anal glands contents. the parasite samples were cha ... | 1991 | 1842405 |
| central nervous system involvement in experimental trypanosomiasis cruzi. | a review of the available literature on central nervous system involvement in experimental trypanosomiasis cruzi is undertaken. from a critical analysis of 26 works on experimental infections with trypanosoma cruzi (23 on the acute phase, 2 on the chronic phase, and one describing sequentially both phases), all supported by neuropathologic studies, it can be concluded that: 1) central nervous system involvement during the acute phase, in the form of encephalitis in multiple foci, with variable i ... | 1991 | 1842407 |
| cell mediated immunity in chagas' disease. trypanosoma cruzi antigens induce suppression of the in vitro proliferative response of mononuclear cells. | the partial suppression of the cell-mediated immune response by trypanosoma cruzi antigens in patients with chagas' disease is demonstrated in a costimulation assay with t. cruzi antigens and mycobacterium tuberculosis purified protein derivative (ppd) or tetanus toxoid (tt). mononuclear cells from 13 patients with chagasic infection without evidence of heart disease, 10 patients with chagasic cardiomyopathy and 7 healthy blood bank donors were stimulated with antigen a (autoclaved epimastigotes ... | 1991 | 1842408 |
| an appraisal of the epidemiology of trypanosoma cruzi serology in brazil. | a large bibliographic survey provided data on trypanosoma cruzi serology covering the period 1948-1984. epidemiological-demographic methods provided an estimate of 11% for the prevalence of positive serology in brazil, by 1984. significant temporal trends were observed for most of the brazilian geographical regions as well as for brazil, as a whole. the parabolic curve that fit best for the entire country, indicates that by 1991, the incidence of new positive serology would be close to zero. thi ... | 1991 | 1842410 |