Publications

analysis of indole compounds in edible basidiomycota species after thermal processing.	methanolic extracts of processed fruiting bodies of six edible mushroom species (basidiomycota) -armillaria mellea, boletus badius, boletus edulis, cantharellus cibarius, lactarius deliciosus collected from natural habitats and pleurotus ostreatus of commercial origin - were analysed for the presence of non-hallucinogenic indole compounds. thermal processing was designed in such a way that it mimicked conditions used for cooking of mushroom dishes, since only a narrow group of mushrooms can be e ...	2012	26434315
assessment of arsenic bioaccessibility in raw and cooked edible mushrooms by a pbet method.	the present study reports arsenic analysis in lentinula edodes, agaricus bisporus and pleurotus ostreatus before and after being cooked. furthermore, arsenic in raw and cooked mushroom was determined in the gastric and gastrointestinal bioaccessible fractions obtained after simulating human digestion by means of an in vitro physiologically based extraction test (pbet). several certified reference materials (srm 1568a, srm 1570a, crm 7503-a, bc211 and ipe-120) were analysed to evaluate the propos ...	2016	26471627
improving the ph-stability of versatile peroxidase by comparative structural analysis with a naturally-stable manganese peroxidase.	versatile peroxidase (vp) from the white-rot fungus pleurotus eryngii is a high redox potential peroxidase of biotechnological interest able to oxidize a wide range of recalcitrant substrates including lignin, phenolic and non-phenolic aromatic compounds and dyes. however, the relatively low stability towards ph of this and other fungal peroxidases is a drawback for their industrial application. a strategy based on the comparative analysis of the crystal structures of vp and the highly ph-stable ...	2015	26496708
can consumption of antioxidant rich mushrooms extend longevity?: antioxidant activity of pleurotus spp. and its effects on mexican fruit flies' (anastrepha ludens) longevity.	the variability of antioxidant capacity of 14 strains of the edible oyster mushroom pleurotus spp. was determined, and the effect of selected mushroom supplements on the longevity of the mexican fruit fly, anastrepha ludens, was evaluated. the antioxidant capacity of the fruiting bodies was determined by three different methods, measuring the free radical scavenging activity of methanolic extracts, the oh radical scavenging capacity, and the total phenol content. the inhibition percentage of the ...	2015	26499817
bioactivity of volatile organic compounds produced by pseudomonas tolaasii.	pseudomonas tolaasii is the main bacterial pathogen of several mushroom species. in this paper we report that strains of p. tolaasii produce volatile substances inducing in vitro mycelia growth inhibition of pleurotus ostreatus and p. eryngii, and agaricus bisporus and p. ostreatus basidiome tissue blocks brown discoloration. p. tolaasii strains produced the volatile ammonia but not hydrogen cyanide. among the volatiles detected by gc-ms, methanethiol, dimethyl disulfide (dmds), and 1-undecene w ...	2015	26500627
levels of platinum group elements and rare-earth elements in wild mushroom species growing in poland.	due to limited data-describing abilities of mushrooms to accumulate platinum group elements (pges) and rare-earth elements (rees), the aim of this study was to determine, by inductively coupled plasma optical emission spectrometry followed by microwave-assisted sample digestion by nitric acid, the content of these elements in 20 mushroom species (10 above ground and 10 growing on wood), mostly edible, collected near a busy trunk road. the highest content of pges in above-ground mushroom species ...	2016	26515437
assessment of bacterial and fungal (hemi)cellulose-degrading enzymes in saccharification of ammonia fibre expansion-pretreated arundo donax.	this study reports enzymatic hydrolysis of the biomass of the giant reed (arundo donax l.) after ammonia fibre expansion (afex) pretreatment. in particular, the capacity of the arabinofuranosidase from the fungus pleurotus ostreatus recombinantly expressed in pichia pastoris rpoabf, its evolved mutant rpoabf f435y/y446f and the endo-cellulase from streptomyces sp. g12 celstrep recombinantly expressed in escherichia coli to enhance the hydrolysis of afex-treated a. donax was investigated, using t ...	2016	26521250
a comprehensive review of tropical milky white mushroom (calocybe indica p&c).	a compressive description of tropical milky white mushroom (calocybe indica p&c var. apk2) is provided in this review. this mushroom variety was first identified in the eastern indian state of west bengal and can be cultivated on a wide variety of substrates, at a high temperature range (30~38℃). however, no commercial cultivation was made until 1998. krishnamoorthy 1997 rediscovered the fungus from tamil nadu, india and standardized the commercial production techniques for the first time in the ...	2015	26539033
differential expression of laccase genes in pleurotus ostreatus and biochemical characterization of laccase isozymes produced in pichia pastoris.	in this study, transcriptome analysis of twelve laccase genes in pleurotus ostreatus revealed that their expression was differentially regulated at different developmental stages. lacc5 and lacc12 were specifically expressed in fruiting bodies and primordia, respectively, whereas lacc6 was expressed at all developmental stages. lacc1 and lacc3 were specific to the mycelial stage in solid medium. in order to investigate their biochemical characteristics, these laccases were heterologously express ...	2015	26539044
fungal biodegradation of anthracene-polluted cork: a comparative study.	the efficiency of cork waste in adsorbing aqueous polycyclic aromatic hydrocarbons (pahs) has been previously reported. biodegradation of contaminated cork using filamentous fungi could be a good alternative for detoxifying cork to facilitate its final processing. for this purpose, the degradation efficiency of anthracene by three ligninolytic white-rot fungi (phanerochaete chrysosporium, irpex lacteus and pleurotus ostreatus) and three non-ligninolytic fungi which are found in the cork itself ( ...	2016	26540209
hydrophilic compounds in liquids of enzymatic hydrolyzed spruce and pine biomass.	organic acids are used for starting compounds in material sciences and in biorefinery, food, fuel, pharmaceutical, and medical industry. here, we provide the data from a biochemical approach made to investigate production of organic acids and isolation of metals from wood, which is the most abundant biomass. spruce and bark, phloem, and heartwood from pine were fermented with either microbes of oyster mushroom (pleurotus ostreatus), baker's yeast, or lactic acid bacteria to improve selective fer ...	2015	26543881
submerged cultivation of mycelium with high ergothioneine content from the culinary-medicinal golden oyster mushroom, pleurotus citrinopileatus (higher basidiomycetes).	the optimization of submerged culture of the culinary-medicinal golden oyster mushroom, pleurotus citrinopileatus, was studied using a one-factor-at-a-time, two-stage stimulation and central composite rotatable design to produce mycelia with high ergothioneine content. the optimal culture conditions for mycelia harvested at day 22 were a temperature of 25°c, an inoculation ratio of 5%, 2% glucose, 0.5% yeast extract, and adjustment of the initial ph value to 10. the biomass and ergothioneine con ...	2015	26559861
chemical composition and character impact odorants in volatile oils from edible mushrooms.	the aim of this study was to investigate the chemical composition and the odor-active components of volatile oils from three edible mushrooms, pleurotus ostreatus, pleurotus eryngii, and pleurotus abalonus, which are well-known edible mushrooms. the volatile components in these oils were extracted by hydrodistillation and identified by gc/ms, gc-olfactometry (gc-o), and aroma extract dilution analysis (aeda). the oils contained 40, 20, and 53 components, representing 83.4, 86.0, and 90.8% of the ...	0	26567951
novel polyol-responsive monoclonal antibodies against extracellular β-d-glucans from pleurotus ostreatus.	β-d-glucans from mushroom strains play a major role as biological response modifiers in several clinical disorders. therefore, a specific assay method is of critical importance to find useful and novel sources of β-d-glucans with anti-tumor activity. hybridoma technology was used to raise monoclonal antibodies (mabs) against extracellular β-d-glucans (ebg) from pleurotus ostreatus. two of these hybridoma clones (3f8_3h7 and 1e6_1e8_b3) secreting mabs against ebg from p. ostreatus were selected a ...	2016	26580487
toughening and its association with the postharvest quality of king oyster mushroom (pleurotus eryngii) stored at low temperature.	toughening-induced textural decay easily occurs in stored mushrooms. the objective of this study was to investigate the textural alteration caused by toughening in relation to other quality attributes of king oyster mushroom (pleurotus eryngii). fresh king oyster mushrooms, packed in low-density polyethylene bags, were stored at different low temperatures for 18 days to measure textural and other quality attributes. it was found that toughening occurred twice during the entire storage time. high ...	2016	26593593
efficient immobilization of a fungal laccase and its exploitation in fruit juice clarification.	the clarification step represents, in fruit juices industries, a bottleneck process because residual phenols cause severe haze formation affecting juice quality and impairing customers acceptance. an enzymatic step can be efficiently integrated in the process, and use of immobilized enzymes entails an economical advantage. in this work, covalent immobilization of recombinant poxa1b laccase from pleurotus ostreatus on epoxy activated poly(methacrylate) beads was optimized thanks to a response sur ...	2016	26593616
long term storage of pleurotus ostreatus and trametes versicolor isolates using different cryopreservation techniques and its impact on laccase activity.	the strain pleurotus ostreatus florida f6, its 45 basidiospore-derived isolates (both monokaryons and dikaryons prepared in our laboratory), trametes versicolor strain ccbas 614 and 22 other t. versicolor isolates obtained from the sporocarps collected in distant localities were successfully preserved for 12 y using perlite and straw cryopreservation protocols. all tested isolates survived a 12-year storage in liquid nitrogen (ln) and their laccase production and poly b411 decolorization capacit ...	2015	26615755
characterisation of the large-scale production process of oyster mushroom (pleurotus ostreatus) with the analysis of succession and spatial heterogeneity of lignocellulolytic enzyme activities.	oyster mushroom (pleurotus ostreatus) lignocellulolytic enzyme activity pattern and variation was investigated in a large-scale facility from spawning until the end of the second flush. in the first cultivation cycle laccase production reached its peak during vegetative growth stage, while manganese-peroxidase showed the highest activity during fruiting body induction. cellulose and hemicellulose degrading enzymes had maximal activity at the beginning of flush and harvest stage. the enzyme activ ...	2015	26615756
the potential of organic substrates based on mushroom substrate and straw to dissipate fungicides contained in effluents from the fruit-packaging industry - is there a role for pleurotus ostreatus?	citrus fruit-packaging plants (fpp) produce large wastewater volumes with high loads of fungicides like ortho-phenylphenol (opp) and imazalil (imz). no methods are in place for the treatment of those effluents and biobeds appear as a viable alternative. we employed a column study to investigate the potential of spent mushroom substrate (sms) of pleurotus ostreatus, either alone or in mixture with straw and soil plus a mixture of straw /soil to retain and dissipate imz and opp. the role of p. ost ...	2016	26624931
antidiabetic effect of polysaccharides from pleurotus ostreatus in streptozotocin-induced diabetic rats.	this study was performed to evaluate the effects of total polysaccharides extracted from pleurotus ostreatus on type 2 diabetes. rats were administered with high-fat diet and streptozotocin (stz) to induce diabetes. the rats were then treated with 100, 200, and 400 mg/kg/d pop or vehicle for 4 weeks. our experiments indicated that pop reduces hyperglycemia and hyperlipidemia levels, improves insulin resistance, and increases glycogen storage by activating gsk3 phosphorylation and glut4 transloca ...	2016	26627601
optimization of arundo donax saccharification by (hemi)cellulolytic enzymes from pleurotus ostreatus.	an enzymatic mixture of cellulases and xylanases was produced by pleurotus ostreatus using microcrystalline cellulose as inducer, partially characterized and tested in the statistical analysis of arundo donax bioconversion. the plackett-burman screening design was applied to identify the most significant parameters for the enzymatic hydrolysis of pretreated a. donax. as the most significant influence during the enzymatic hydrolysis of a. donax was exercised by the temperature (°c), ph, and time, ...	2015	26634214
β-glucan-based cream (containing pleuran isolated from pleurotus ostreatus) in supportive treatment of mild-to-moderate atopic dermatitis.	atopic dermatitis (ad) is one of the most common chronic inflammatory skin diseases with serious impact on quality of life. β-glucans are natural substances with potent immunomodulatory and anti-inflammatory activity.	2016	26654776
downscaling the in vitro test of fungal bioremediation of polycyclic aromatic hydrocarbons: methodological approach.	the miniaturization and optimization of a white rot fungal bioremediation experiment is described in this paper. the optimized procedure allows determination of the degradation kinetics of anthracene. the miniaturized procedure requires only 2.5 ml of culture medium. the experiment is more precise, robust, and better controlled comparing it to classical tests in flasks. using this technique, different parts, i.e., the culture medium, the fungi, and the cotton seal, can be analyzed. a simple samp ...	2016	26660875
effect of solid state fermentation medium optimization on pleurotus ostreatus laccase production.	the objective of this work was to increase laccase production by pleurotus ostreatus plab through culture medium optimization using solid state culture conditions. increased laccase activity was obtained through design of experiments (doe) using the taguchi orthogonal array (oa). seven factors, viz. lignocellulose, glucose, yeast extract, peptone, kh(2)po(4), mgso(4) 7h(2)o and mnso(4) h(2)o at three levels and ph at two levels. oa layout of l18 (2(1) x 3(7)) was selected for the proposed experi ...	2015	26680722
hypoglycemic and antidiabetic effect of pleurotus sajor-caju aqueous extract in normal and streptozotocin-induced diabetic rats.	pleurotus sajor-caju (psc) is an edible oyster mushroom featuring high nutritional values and pharmacological properties. objective. to investigate the hypoglycemic and antidiabetic effects of single and repeated oral administration of psc aqueous extract in normal and diabetic rats.	2015	26682215
identification of the nucleophile catalytic residue of gh51 α-l-arabinofuranosidase from pleurotus ostreatus.	in this study, the recombinant α-l-arabinofuranosidase from the fungus pleurotus ostreatus (rpoabf) was subjected to site-directed mutagenesis in order to identify the catalytic nucleophile residue. based on bioinformatics and homology modelling analyses, e449 was revealed to be the potential nucleophilic residue. thus, the mutant e449g of poabf was recombinantly expressed in pichia pastoris and its recombinant expression level and reactivity were investigated in comparison to the wild-type. the ...	2015	26690659
effect of supplementing crop substrate with defatted pistachio meal on agaricus bisporus and pleurotus ostreatus production.	this work assesses the agronomic performance of defatted pistachio meal, after oil extraction, as a nutritional substrate supplement when growing the mushroom species agaricus bisporus (lange) imbach and pleurotus ostreatus (jacq.) p. kumm. materials were applied at different doses at spawning. along with non-supplemented substrates, commercial nutritional supplements were used as controls. proximate analysis of mushrooms is also considered.	2016	26692380
morphological and molecular characterization of yellow oyster mushroom, pleurotus citrinopileatus, hybrids obtained by interspecies mating.	pleurotus citrinopileatus (yellow oyster mushroom) has an attractive shape and yellow colour but the fragile texture complicates packaging, and its strong aroma is unappealing to consumers. this study aimed to improve the characteristics and yield of p. citrinopileatus by interspecies mating between monokaryotic cultures of p. citrinopileatus and p. pulmonarius. ten monokaryon cultures of the parental lines were crossed in all combinations to obtain hybrids. eleven compatible mating pairs were o ...	2016	26745978
antioxidant capacity and total phenolics content of the fruiting bodies and submerged cultured mycelia of sixteen higher basidiomycetes mushrooms from india.	the fruiting bodies and the submerged cultured mycelia of 16 higher basidiomycetes mushrooms- agaricus bisporus, armillaria mellea, auricularia auricula-judae, ganoderma applanatum, g. lucidum, laetiporus sulphureus, lentinus tigrinus, lycoperdon pyriforme, phellinus linteus, pleurotus ostreatus, p. sajor-caju, polyporus arcularius, russula brevipes, schizophyllum commune, sparassis crispa, and spongipellis unicolor-from different taxonomic groups were examined for their antioxidant capacity (ao ...	2015	26756185
a study on 17alpha-ethinylestradiol metabolism in rat and pleurotus ostreatus.	17α-ethinylestradiol (ee2) is an endocrine disruptor that is an ingredient of oral contraceptives. here, ee2 metabolism catalyzed by cytochromes p450 (cyp) was studied. two model organisms, rat and ligninolytic fungus pleurotus ostreatus, were used.	2015	26757108
study on vitamin d₂ stability in dried mushrooms during drying and storage.	the main objective of this work was to determine the stability of vitamin d2 in dried mushrooms agaricus bisporus, pleurotus ostreatus and lentinula edodes during storage, as well as to examine the possibility of inducing vitamin d2 production in dried mushrooms by uvb irradiation. after 1.5 year storage of dried mushrooms, the level of vitamin d2 in button mushrooms was found to be 6.90 μg/g dw, which is a 48.32% of initial level of vitamin d2. in the case of dried oyster and shiitake mushrooms ...	2016	26775962
environmental assessment of the degradation potential of mushroom fruit bodies of pleurotus ostreatus (jacq.: fr.) p. kumm. towards synthetic azo dyes and contaminating effluents collected from textile industries in karnataka, india.	pleurotus ostreatus (jacq.: fr.) p. kumm. is one of the edible mushrooms currently gaining attention as environmental restorer. the present study explores the potential of p. ostreatus (jacq.: fr.) p. kumm. in degradation of textile dyes and effluents. the mushroom cultivation was carried out using paddy bed as substrate. the fully grown mushroom fruit bodies were used as a bioremediation agent against two industrially important azo dyes such as nylon blue and cotton yellow and few effluents col ...	2016	26818015
class i hydrophobin vmh2 adopts atypical mechanisms to self-assemble into functional amyloid fibrils.	hydrophobins are fungal proteins whose functions are mainly based on their capability to self-assemble into amphiphilic films at hydrophobic-hydrophilic interfaces (hhi). it is widely accepted that class i hydrophobins form amyloid-like structures, named rodlets, which are hundreds of nanometers long, packed into ordered lateral assemblies and do not exhibit an overall helical structure. we studied the self-assembly of the class i hydrophobin vmh2 from pleurotus ostreatus in aqueous solutions by ...	2016	26828412
the effects of different substrates on the growth, yield, and nutritional composition of two oyster mushrooms (pleurotus ostreatus and pleurotus cystidiosus).	the study was conducted to compare the effects of different agro-wastes on the growth, yield, and nutritional composition of oyster mushrooms pleurotus ostreatus (po) and pleurotus cystidiosus (pc). seven substrate formulas including sawdust (sd), corncob (cc), sugarcane bagasse (sb) alone and in combination of 80 : 20, 50 : 50 ratio between sd and cc, sd and sb were investigated. the results indicated that different substrate formulas gave a significant difference in total colonization period, ...	2015	26839502
improvement of tolerance to lead by filamentous fungus pleurotus ostreatus hau-2 and its oxidative responses.	wastewater contaminated with heavy metals is a world-wide concern. one biological treatment strategy includes filamentous fungi capable of extracellular adsorption and intracellular bioaccumulation. here we report that an acclimated strain of filamentous fungus pleurotus ostreatus hau-2 can withstand pb up to 1500 mg l(-1) pb, conditions in which the wildtype strain cannot grow. the acclimated strain grew in liquid culture under 500 mg l(-1) pb without significant abnormity in biomass and morpho ...	2016	26891354
iron translocation in pleurotus ostreatus basidiocarps: production, bioavailability, and antioxidant activity.	translocation of minerals from substrate to mushrooms can change the medicinal characteristics, commercial value, and biological efficiency of mushroom. in the present study, we demonstrated that addition of iron to the substrate reduces the yield of pleurotus ostreatus mushroom. the biological efficiency of the mushroom varied from 36.53% on the unsupplemented substrate to 2.08% for the substrate with 500 mg/kg iron added. the maximum iron concentration obtained for mushroom was 478.66 mg/kg (d ...	0	26909996
diffusional and transcriptional mechanisms involved in laccases production by pleurotus ostreatus cp50.	the independent effects of hydrodynamic stress (assessed as the energy dissipation/circulation function, edcf) and dissolved oxygen tension (dot) on the growth, morphology and laccase production by pleurotus ostreatus cp50 were studied using a 3(2) factorial design in a 10l reactor. a bell-shape function for fungus growth between 8 and 22% dot was observed, as well as a significant negative effect on laccase production and the expression of poxc, the gene encoding for the most abundant laccase p ...	2016	26924241
a secretomic view of woody and nonwoody lignocellulose degradation by pleurotus ostreatus.	pleurotus ostreatus is the second edible mushroom worldwide, and a model fungus for delignification applications, with the advantage of growing on woody and nonwoody feedstocks. its sequenced genome is available, and this gave us the opportunity to perform proteomic studies to identify the enzymes overproduced in lignocellulose cultures.	2016	26933449
[molecular cloning and characterization of two oxidative stress related genes from medicinal fungus polyporus umbellatus].	to clone the nadph gene (punox) and glyoxal oxidase gene (puglox) from a medicinal fungus polyporus umbellatus, and to carry out the bioinformatic analysis.	2015	26939456
effects of overexpression of pkac genes on expressions of lignin-modifying enzymes by pleurotus ostreatus.	we studied the role of genes encoding the camp-dependent protein kinase a catalytic subunit (pkac) in the ligninolytic system in pleurotus ostreatus. the wild-type p. ostreatus strain pc9 has two pkac-encoding genes: pkac1 and pkac2 (protein id 114122 and 85056). in the current study, pkac1 and pkac2 were fused with a β-tubulin promoter and introduced into strain pc9 to produce the overexpression strains pkac1-97 and pkac2-69. these strains showed significantly higher transcription levels of iso ...	2016	26979984
the quality changes of postharvest mulberry fruit treated by chitosan-g-caffeic acid during cold storage.	this study aimed to characterize the effects of chitosan-g-caffeic acid (cts-g-ca) on improving the quality and extending the shelf life of postharvest mulberry fruit during storage at 4 °c for 18 d. cts-g-ca was enzymatically synthesized using laccase from pleurotus ostreatus as a catalyst. the synergistic effects of cts-g-ca treatment on mulberry fruit were evaluated using a co-toxicity factor (cf). the results showed that the rotting rate of cts-g-ca-treated fruit was 37.67% (compared with th ...	2016	26992122
enzymatic modification of chitosan by cinnamic acids: antibacterial activity against ralstonia solanacearum.	this study aimed to identify chitosan polymers that have antibacterial activity against the bacterial wilt pathogen. the chitosan polymers were enzymatically synthesized using chitosan and five cinnamic acids (cads): caffeic acid (ca), ferulic acid (fa), cinnamic acid (cia), p-coumaric acid (coa) and chlorogenic acid (cha), using laccase from pleurotus ostreatus as a catalyst. the reaction was performed in a phosphate buffered solution under heterogenous reaction conditions. the chitosan derivat ...	2016	26993531
occupational asthma in a fruit and vegetables vendor.	this study presents a case report of a woman running a fruit and vegetables market stall, occupationally exposed to mushroom spores, who developed asthmatic symptoms. basidiospores are allergens that can give rise to sensitization and subsequently trigger an allergic reaction, such as contact dermatitis, rhino-conjunctivitis, asthma and hypersensitivity pneumonitis.	0	27015024
biodegradation of carbamazepine and clarithromycin by trichoderma harzianum and pleurotus ostreatus investigated by liquid chromatography - high-resolution tandem mass spectrometry (fticr ms-irmpd).	in this study, the capability of pharmaceutical biodegradation of fungus trichoderma harzianum was evaluated through the comparison with the well-known biodegradation capability of white-rot fungus pleurotus ostreatus. the study was performed in aqueous phase under aerobic conditions, using two of the most frequently detected drugs in water bodies: carbamazepine and clarithromycin, with concentrations commonly found in treated wastewater (4μg/l and 0.03μg/l respectively). for the first time, we ...	2016	27039063
evaluation of pb (ii) biosorption utilizing sugarcane bagasse colonized by basidiomycetes.	the contamination of water resources by metallic ions is a serious risk to public health and the environment. therefore, a great emphasis has been given to alternative biosorption methods that are based on the retention of aqueous-solution pollutants; in the last decades, several agricultural residues have been explored as low-cost adsorbent. in this study, the ability of pb (ii) biosorption using sugarcane bagasse modified by different fungal species was evaluated. the presence of carbonyl, hyd ...	2016	27063515
modulatory effect of pleurotus ostreatus on oxidant/antioxidant status in 7, 12-dimethylbenz (a) anthracene induced mammary carcinoma in experimental rats--a dose-response study.	worldwide, breast cancer is the second most prevalent cancer among women and its incidence is increasing alarmingly.	2016	27072268
phylogenetic relationship of two popular edible pleurotus in china, bailinggu (p. eryngii var. tuoliensis) and xingbaogu (p. eryngii), determined by its, rpb2 and ef1α sequences.	the aims of this study are to assess the utility of the internal transcribed spacer (its) region, and partial translation elongation factor (ef1α) and rna polymerase ii (rpb2) genes, for differentiation of bailinggu, p. eryngii, and p. nebrodensis; to reconstruct phylogenetic relationships between the three species; and to confirm the taxonomic status of bailinggu based on ribosomal and protein-coding genes. pairwise genetic distances between bailinggu, p. eryngii, and related pleurotus strains ...	2016	27075657
comparative study of the physicochemical, compositional and functional properties of eight flours obtained from different plant materials found in colombia.	the physicochemical, compositional and functional properties of flour from green plantains (musa acuminata) of the large green plantain variety, oyster mushrooms (pleorotus ostreatus), pineapple peel (ananas comosus) of the 'apple pineapple' variety, yellow peas (pisum sativum), chickpeas (cicer arietinum), whole grain rice (oryza sativa), whole grain corn (zea mays) and whole grain white quinoa (chenopodium quinoa) were evaluated by using one-way analysis of variance, pearson correlations and p ...	2016	27091150
comparative cold shock expression and characterization of fungal dye-decolorizing peroxidases.	dye-decolorizing peroxidases (dyps) from auricularia auricula-judae, bjerkandera adusta, pleurotus ostreatus and marasmius scorodonius (basidiomycota) were expressed in escherichia coli using the cold shock-inducible expression system pcold i dna. functional expression was achieved without the addition of hemin or the co-expression of any chaperones. the presence or absence of the native signal sequence had a strong impact on the success of the expression, but the effect was not consistent for t ...	2016	27106285
deproteinization of water-soluble ß-glucan during acid extraction from fruiting bodies of pleurotus ostreatus mushrooms.	some ß-glucans can be easily extracted from basidiomycete mushrooms but commonly used extraction procedures are not satisfactory. a simultaneous method for acid extraction and deproteinization in the case of pleurotus ostreatus was developed using response surface methodology. the optimized extraction conditions proposed here (30°c, 3.8% hcl, 300min, stirring) allow for the simultaneous extraction and deproteinization of polysaccharides. additionally, the acid extraction yield was 7 times greate ...	2016	27112879
mutual interactions of pleurotus ostreatus with bacteria of activated sludge in solid-bed bioreactors.	white rot fungi are well known for their ability to degrade xenobiotics in pure cultures but few studies focus on their performance under bacterial stress in real wastewaters. this study investigated mutual interactions in co-cultures of pleurotus ostreatus and activated sludge microbes in batch reactors and different culture media. under the bacterial stress an increase in the dye decolorization efficiency (95 vs. 77.1 %) and a 2-fold elevated laccase activity (156.7 vs. 78.4 ul(-1)) were obser ...	2016	27116960
comparative and transcriptional analysis of the predicted secretome in the lignocellulose-degrading basidiomycete fungus pleurotus ostreatus.	fungi interact with their environment by secreting proteins to obtain nutrients, elicit responses and modify their surroundings. because the set of proteins secreted by a fungus is related to its lifestyle, it should be possible to use it as a tool to predict fungal lifestyle. to test this hypothesis, we bioinformatically identified 538 and 554 secretable proteins in the monokaryotic strains pc9 and pc15 of the white rot basidiomycete pleurotus ostreatus. functional annotation revealed unknown f ...	2016	27117896
anti-inflammatory effect of aqueous extracts of spent pleurotus ostreatus substrates in mouse ears treated with 12-o-tetradecanoylphorbol-13-acetate.	to evaluate the application of spent pleurotus ostreatus substrates, enriched or not with medicinal herbs, as a source of anti-inflammatory compounds.	2017	27127316
treatment of petroleum drill cuttings using bioaugmentation and biostimulation supplemented with phytoremediation.	this study sought to compare the effectiveness of bioaugmentation and biostimulation, as well as the combination of both techniques, supplemented with phytoremediation, in the decontamination of petroleum drill cuttings. drill cuttings with relatively low concentration of total petroleum hydrocarbons (tph) and metals were mixed with soil in the ratio 5:1 and treated with three different combinations of the bioremediation options. option a entailed bioaugmentation supplemented with phytoremediati ...	2016	27128694
limits of versatility of versatile peroxidase.	although mn(2+) is the most abundant substrate of versatile peroxidases (vps), repression of pleurotus ostreatus vp1 expression occurred in mn(2+)-sufficient medium. this seems to be a biological contradiction. the aim of this study was to explore the mechanism of direct oxidation by vp1 under mn(2+)-deficient conditions, as it was found to be the predominant enzyme during fungal growth in the presence of synthetic and natural substrates. the native vp1 was purified and characterized using three ...	2016	27129968
food-derived hydrophilic antioxidant ergothioneine is distributed to the brain and exerts antidepressant effect in mice.	clinically used antidepressants suffer from various side effects. therefore, we searched for a safe antidepressant with minimal side effects among food ingredients that are distributed to the brain. here, we focused on ergo (ergothioneine), which is a hydrophilic antioxidant and contained at high levels in edible golden oyster mushrooms. ergo is a typical substrate of carnitine/organic cation transporter octn1/slc22a4, which is expressed in the brain and neuronal stem cells, although little is k ...	2016	27134772
an aryl-alcohol oxidase of pleurotus sapidus: heterologous expression, characterization, and application in a 2-enzyme system.	aryl-alcohol oxidases (aaos) are enzymes supporting the degradation of lignin by fungal derived class ii peroxidases produced by white-rot fungi. aaos are able to generate h2o2 as a by-product via oxidation of an aryl-alcohol into its correspondent aldehyde. in this study, an aao was heterologously expressed in a basidiomycete host for the first time. the gene for an aao of the white-rot fungus pleurotus sapidus, a close relative to the oyster mushroom pleurotus ostreatus, was cloned into an exp ...	2016	27138199
enzymatic formation of compound-k from ginsenoside rb1 by enzyme preparation from cultured mycelia of armillaria mellea.	minor saponins or human intestinal bacterial metabolites, such as ginsenosides rg3, f2, rh2, and compound k, are more pharmacologically active than major saponins, such as ginsenosides rb1, rb2, and rc. in this work, enzymatic hydrolysis of ginsenoside rb1 was studied using enzyme preparations from cultured mycelia of mushrooms.	2016	27158230
comparative assessment of fungal augmentation treatments of a fine-textured and historically oil-contaminated soil.	the removal of aged hydrophobic contaminants from fine-textured soils is a challenging issue in remediation. the objective of this study was to compare the efficacy of augmentation treatments to that of biostimulation in terms of total aliphatic hydrocarbon (tah) and toxicity removal from a historically contaminated clay soil and to assess their impact on the resident microbial community. to this aim, pleurotus ostreatus, botryosphaeria rhodina and a combination of both were used as the inoculan ...	2016	27220102
exploring glycoside hydrolases and accessory proteins from wood decay fungi to enhance sugarcane bagasse saccharification.	glycoside hydrolases (ghs) and accessory proteins are key components for efficient and cost-effective enzymatic hydrolysis of polysaccharides in modern, biochemically based biorefineries. currently, commercialized ghs and accessory proteins are produced by ascomycetes. however, the role of wood decay basidiomycetes proteins in biomass saccharification has not been extensively pursued. wood decay fungi degrade polysaccharides in highly lignified tissues in natural environments, and are a promisin ...	2016	27222665
carbohydrate changes during growth and fruiting in pleurotus ostreatus.	the carbohydrate distribution in mushrooms is reported changing greatly in its different regions during growth and fruiting. in this study, the carbohydrate distribution in the compost and fruiting bodies of pleurotus ostreatus was analysed. sugar, polyol, polysaccharide, and chitin content during different growth phases and in different regions of the mushroom were determined. results indicate that trehalose, mannitol, and glucose were first accumulated in the compost and then decreased during ...	2017	27268245
protective effect of antioxidant extracts from grey oyster mushroom, pleurotus pulmonarius (agaricomycetes), against human low-density lipoprotein oxidation and aortic endothelial cell damage.	this study evaluated the in vitro antioxidant capacities of extracts from pleurotus pulmonarius via folin-ciocalteu, 1,1-diphenyl-2-picrylhydrazyl free radical scavenging, metal chelating, cupric ion reducing antioxidant capacity, and lipid peroxidation inhibition assays. extract compositions were determined by phenol-sulfuric acid; coomassie plus (bradford) protein; spectroquant zinc, copper, and manganese test assays; and liquid chromatography-tandem mass spectrometry (lc/ms/ms) and gas chroma ...	2016	27279533
transposable elements versus the fungal genome: impact on whole-genome architecture and transcriptional profiles.	transposable elements (tes) are exceptional contributors to eukaryotic genome diversity. their ubiquitous presence impacts the genomes of nearly all species and mediates genome evolution by causing mutations and chromosomal rearrangements and by modulating gene expression. we performed an exhaustive analysis of the te content in 18 fungal genomes, including strains of the same species and species of the same genera. our results depicted a scenario of exceptional variability, with species having ...	2016	27294409
iron bioavailability from cereal products enriched with pleurotus ostreatus mushrooms in rats with induced anaemia.	oyster mushroom pleurotus ostreatus is good source of iron. however, there is a limited data concerning bioavailability of iron from oyster mushroom and also cereal products containing this mushroom. the aim of this study was to assess bioavailability of iron from products with an addition of pleurotus ostreatus in male rats with anaemia.	2016	27294638
a macrosphelide as the unexpected product of a pleurotus ostreatus strain-mediated biotransformation of halolactones containing the gem-dimethylcyclohexane ring. part 1.	the aim of the study was to obtain new compounds during biotransformation of two halocompounds, the δ-bromo and δ-iodo-γ-bicyclolactones 1 and 2. unexpectedly pleurotus ostreatus produced together with the hydroxylactone, 2-hydroxy-4,4-dimethyl-9-oxabicyclo[4.3.0]nonane-8-one (3), its own metabolite (3s,9s,15s)-(6e,12e)-3,9,15-trimethyl-4,10,16-trioxacyclohexa-deca-6,12-diene-1,5,8,11,14-pentaone (4). the method presented here, in which this macrosphelide 4 was obtained by biotransformation, has ...	2016	27376255
effects of processing on carbendazim residue in pleurotus ostreatus.	samples of pleurotus ostreatus were exposed to fungicide carbendazim to study the effect of processing on the residues. in most cases, processing operations led to a significant decrease in residue levels in the finished products, particularly through washing, drying, and cooking processes. the results indicated that rinsing under running tap water led to more than 70.30% loss in carbendazim residues. when dried under sunlight could remove more than 70.30% residues. there was a 63.90-97.14% redu ...	2016	27386113
pleurotus ostreatus decreases cornstalk lignin content, potentially improving its suitability for animal feed.	the capacity of pleurotus ostreatus to degrade lignin was investigated in the fermentation of cornstalk. cornstalk was incubated with p. ostreatus for 30 days, and acid-soluble and acid-insoluble lignins were assessed. the microscopic structure of cornstalk samples was studied by scanning electron microscopy (sem), and spectroscopic characteristics were measured by x-ray diffraction (xrd), fourier transform infrared spectroscopy (ftir) and solid state nuclear magnetic resonance ((13) c nmr) spec ...	2017	27417952
vmh2 hydrophobin as a tool for the development of "self-immobilizing" enzymes for biosensing.	self-assembling proteins forming amyloid fibrils are promising candidates for the fabrication of biomaterials, due to the chemical and mechanical stability of their structures. among potential applications, their use as platforms for enzyme immobilization is rapidly gathering attention. in this work, we demonstrate that the production of the enzyme glutathione-s-transferase (gst) fused to the class i hydrophobin vmh2 from pleurotus ostreatus represents an invaluable tool for the development of s ...	2017	27427236
evaluation of the antioxidant activity of aqueous and methanol extracts of pleurotus ostreatus in different growth stages.	total polyphenols and flavonoids contents, as well as ferric reducing antioxidant power (frap), metal ions chelating activity, reducing power assay and scavenging activity of dpph and abts radicals in aqueous and methanolic extracts obtained from mycelium, primordium, and fruiting body of pleurotus ostreatus in both fresh as dry, were evaluated. the total polyphenol content of dried samples was higher in aqueous extracts obtained both in room temperature and boiling. the total polyphenol content ...	2016	27462314
the anti-adipogenic activity of a new cultivar, pleurotus eryngii var. ferulae 'beesan no. 2', through down-regulation of ppar γ and c/ebp α in 3t3-l1 cells.	adipogenesis is one of the cellular processes and a highly controlled program. nowadays, inhibition of adipogenesis has received attention as an effective way to regulate obesity. in the current study, we investigated the inhibition effect of a chloroform extract of pleurotus eryngii var. ferulae 'beesan no. 2' (cebt) on adipogenesis in 3t3-l1 murine preadipocytes. pleurotus eryngii var. ferulae is one of many varieties of king oyster mushroom and has been reported to have various biological act ...	2016	27470278
enzymatic cross-linking of carboxymethylpullulan grafted with ferulic acid.	carboxymethylpullulan (cmp) has been modified in a two-step grafting reaction of ferulic acid (fa). acid adipic dihydrazyde (adh) was first reacted with fa activated with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (edc). then the product of this first reaction was reacted with cmp (activated with edc). grafted polysaccharides structure was confirmed by ftir and (1)h nmr spectroscopy. analyses by size-exclusion chromatography (sec) coupling on-line with a multi-angle light scatte ...	2016	27474545
a patient with mushroom allergy; a new etiological agent of kounis syndrome.	kounis syndrome (ks) is a rarely diagnosed condition which should always be kept in mind when an acute myocardial infarction (ami) happens in the context of anaphylactic reactions. we report a case of a 31-year old female; 2 hours after the ingestion of the mushroom (pleurotus ostreatus); she experienced nausea, stomachache, vomiting, dyspnea and chest pain. electrocardiogram (ecg) showed an st segment elevation in d1, avl, precordial leads v1-v4. the blood analysis revealed high levels of ck-mb ...	2016	27481084
antioxidant, anti-inflammatory, and antitumor activities of cultured mycelia and fruiting bodies of the elm oyster mushroom, hypsizygus ulmarius (agaricomycetes).	ethanoic extracts from the fruiting bodies and mycelia of the elm oyster mushroom, hypsizygus ulmarius, were evaluated for their antioxidant, anti-inflammatory, and antitumor properties. ethnolic extracts of fruiting body and mycelia showed 88%, 85%, 71%, and 85%, 65%, 70% 2,2-diphenyl-1-picrylhydrazyl, hydroxyl (dpph) and 2,2'-azinobis (3-ethyl benzothiazolin-6-sulfonic acid) (abts) radical-scavenging activities, respectively, at a concentration of 1000 µg/ml. the anti-inflammatory activity was ...	2016	27481157
exploring the medicinal potential of the fruit bodies of oyster mushroom, pleurotus ostreatus (agaricomycetes), against multidrug-resistant bacterial isolates.	bacterial resistance to present-generation antibiotics is increasing drastically, which has become a major public health concern. the present study focuses on demonstrating the antimicrobial potential of fruit bodies of the culinary/medicinal oyster mushroom pleurotus ostreatus against clinical pathogens. five bacterial isolates were collected from sagar hospital in bangalore, india. the collected strains were grown on selective and differential media and antibiotic susceptibility testing was ap ...	2016	27481158
study of antimicrobial and cytotoxic potential of the oyster mushroom pleurotus ostreatus cv. florida (agaricomycetes).	this work was undertaken to evaluate in vitro antimicrobial and cytotoxic potential of pleurotus ostreatus cv. florida. mushroom basidiocarps were extracted in water:ethanol (1:1, v/v), and the resulting extract was subjected to antimicrobial studies against escherichia coli, pseudomonas aeruginosa, staphylococcus aureus, klebsiella oxytoca, bacillus subtilis, and candida albicans. cytotoxic potential on viable human leukocytes was studied. in vitro results showed excellent antimicrobial and cyt ...	2016	27481298
polysaccharides from pleurotus ostreatus alleviate cognitive impairment in a rat model of alzheimer's disease.	this study was conducted to evaluate the effects of polysaccharides extracted from pleurotus ostreatus (pop) on d-galactose and alcl3-induced cognitive impairments. a behavioral test suggested that pop significantly decreased escape latency and increased crossing parameters of platform quadrant in a morris water maze test. furthermore, pop decreased error numbers and increased passive avoidance latency in a step-down test. biochemical examinations revealed that pop significantly elevated superox ...	2016	27498414
marker recycling via 5-fluoroorotic acid and 5-fluorocytosine counter-selection in the white-rot agaricomycete pleurotus ostreatus.	of all of the natural polymers, lignin, an aromatic heteropolymer in plant secondary cell walls, is the most resistant to biological degradation. white-rot fungi are the only known organisms that can depolymerize or modify wood lignin. investigating the mechanisms underlying lignin biodegradation by white-rot fungi would contribute to the ecofriendly utilization of woody biomass as renewable resources in the future. efficient gene disruption, which is generally very challenging in the white-rot ...	2016	27567720
biodegradation of 2-naphthalensulfonic acid polymers by white-rot fungi: scale-up into non-sterile packed bed bioreactors.	this paper presents a first scale up under non-sterile conditions of the biodegradation process of 2-naphthalensulfonic acid polymers (nsap) contained in a petrochemical wastewater by two white-rot fungi (bjerkandera adusta and pleurotus ostreatus). the biodegradation experiment was conducted first in flasks and then in packed-bed bioreactors filled with inert and biodegradable carriers (straw), the latter acting as both physical support and carbon source. reactor inoculated with p. ostreatus at ...	2016	27587355
pleurotus citrinopileatus polysaccharide induces activation of human dendritic cells through multiple pathways.	many edible mushrooms have become attractive as "health foods" and as source materials for immunomodulators. to increase our insight in the immune-modulatory properties of a polysaccharide of the oyster mushroom pleurotus citrinopileatus, pcps, we analyzed its effects on the function of human dendritic cells (dcs). we showed that pcps induces upregulation of the surface maturation markers cd80, cd86 and hla-dr on dcs, indicating its potential to induce dc maturation. in addition, pcps stimulates ...	2016	27592080
oral administration of an aqueous extract from the oyster mushroom pleurotus ostreatus enhances the immunonutritional recovery of malnourished mice.	mushroom nutriceutical components have lately attracted interest for developing immunonutritional support. however, there is relatively little information pertaining to the use of mushroom preparations for modulating the metabolic and immunological disorders associated to malnutrition. this study was aimed to evaluate the effects of oral administration of an aqueous extract (cw-p) from pleurotus ostreatus on the recovery of biochemical and immunological functions of malnourished mice. 8-week old ...	2016	27608429
laccase-mediated transformation of triclosan in aqueous solution with metal cations and humic acid.	triclosan (tcs) is a broad-spectrum antimicrobial agent that is found extensively in natural aquatic environments. enzyme-catalyzed oxidative coupling reactions (ecocrs) can be used to remove tcs in aqueous solution, but there is limited information available to indicate how metal cations (mcs) and natural organic matter (nom) influence the environmental fate of tcs during laccase-mediated ecocrs. in this study, we demonstrated that the naturally occurring laccase from pleurotus ostreatus was ef ...	2017	27640762
mushroom extracts decrease bone resorption and improve bone formation.	mushroom extracts have shown promising effects in the treatment of cancer and various chronic diseases. osteoporosis is considered one of the most widespread chronic diseases, for which currently available therapies show mixed results. in this research we investigated the in vitro effects of water extracts of the culinary-medicinal mushrooms trametes versicolor, grifola frondosa, lentinus edodes, and pleurotus ostreatus on a mc3t3-e1 mouse osteoblast-like cell line, primary rat osteoblasts, and ...	2016	27649725
characteristics of uranium biosorption from aqueous solutions on fungus pleurotus ostreatus.	uranium(vi) biosorption from aqueous solutions was investigated in batch studies by using fungus pleurotus ostreatus biomass. the optimal biosorption conditions were examined by investigating the reaction time, biomass dosage, ph, temperature, and uranium initial concentration. the interaction between fungus biomass and uranium was confirmed using fourier transformed infrared (ft-ir), scanning electronic microscopy energy dispersive x-ray (sem-edx), and x-ray photoelectron spectroscopy (xps) ana ...	2016	27662852
use of swine wastewater as alternative substrate for mycelial bioconversion of white rot fungi.	seven white rot fungal species were tested for growth as mycelia using swine wastewater (sw), an agro-waste with tremendous environmental footprint, as the sole nutrient source. the sw contained high concentrations of carbon and nitrogen components, which could support nutritional requirements for mycelial growth. out of the seven species, pleurotus ostreatus and hericium erinaceus were successfully cultivated on the sw medium using solid-state fermentation. response surface methodology was empl ...	2017	27696140
optimization of substrate preparation for oyster mushroom (pleurotus ostreatus) cultivation by studying different raw materials and substrate preparation conditions (composting: phases i and ii).	in recent years, oyster mushroom (pleurotus ostreatus) has become one of the most cultivated mushrooms in the world, mainly in brazil. among many factors involved in a mushroom production, substrate preparation is the most critical step, which can be influenced by composting management techniques. looking forward to optimizing the substrate preparation process, were tested different composting conditions (7 and 14 days of composting with or without conditioning), potential raw materials (decumbe ...	2016	27696289
analysis of synonymous codon usage patterns in the edible fungus volvariella volvacea.	in this study, trends in synonymous codons usage of volvariella volvecea have been first examined by analysis of complete coding sequences and gene chip data. the results showed that gc content at three codon positions are obviously different and there were several factors shaping the codon usage of v. volvacea genes, including base composition. the comparison of codon usage among four edible fungi such as v. volvacea, agaricus bisporus, coprinopsis cinerea, and pleurotus ostreatus indicated tha ...	2016	27696508
identification and characterization of small noncoding rnas in genome sequences of the edible fungus pleurotus ostreatus.	noncoding rnas (ncrnas) have been identified in many fungi. however, no genome-scale identification of ncrnas has been inventoried for basidiomycetes. in this research, we detected 254 small noncoding rnas (sncrnas) in a genome assembly of an isolate (ccef00389) of pleurotus ostreatus, which is a widely cultivated edible basidiomycetous fungus worldwide. the identified sncrnas include snrnas, snornas, trnas, and mirnas. snrna u1 was not found in ccef00389 genome assembly and some other basidiomy ...	2016	27703969
growth and yield performance of pleurotus ostreatus (jacq. fr.) kumm (oyster mushroom) on different substrates.	mushroom cultivation is reported as an economically viable bio-technology process for conversion of various lignocellulosic wastes. given the lack of technology know-how on the cultivation of mushroom, this study was conducted in wondo genet college of forestry and natural resource, with the aim to assess the suitability of selected substrates (agricultural and/or forest wastes) for oyster mushroom cultivation. accordingly, four substrates (cotton seed, paper waste, wheat straw, and sawdust) wer ...	2016	27704469
biosynthesis of nanoparticles of metals and metalloids by basidiomycetes. preparation of gold nanoparticles by using purified fungal phenol oxidases.	the work shows the ability of cultured basidiomycetes of different taxonomic groups-lentinus edodes, pleurotus ostreatus, ganoderma lucidum, and grifola frondosa-to recover gold, silver, selenium, and silicon, to elemental state with nanoparticles formation. it examines the effect of these metal and metalloid compounds on the parameters of growth and accumulation of biomass; the optimal cultivation conditions and concentrations of the studied ion-containing compounds for recovery of nanoparticle ...	2017	27717965
effect of textile dyes on activity and differential regulation of laccase genes from pleurotus ostreatus grown in submerged fermentation.	this research was conducted to extend the knowledge on the differential regulation of laccase genes in response to dyes. in order to accomplish this, we analyzed both, the expression of five laccase genes by real time rt-qpcr, and also the laccase activity and isoforms patterns during the time-course of a pleurotus ostreatus submerged fermentation supplemented with either acetyl yellow g (ayg) or remazol brilliant blue r (rbbr) dyes. for the purpose of obtaining a stable reference gene for optim ...	2016	27718214
development of mushroom-based cosmeceutical formulations with anti-inflammatory, anti-tyrosinase, antioxidant, and antibacterial properties.	the cosmetic industry is in a constant search for natural compounds or extracts with relevant bioactive properties, which became valuable ingredients to design cosmeceutical formulations. mushrooms have been markedly studied in terms of nutritional value and medicinal properties. however, there is still slow progress in the biotechnological application of mushroom extracts in cosmetic formulations, either as antioxidants, anti-aging, antimicrobial, and anti-inflammatory agents or as hyperpigment ...	2016	27754433
effect of different cooking methods on nutritional value and antioxidant activity of cultivated mushrooms.	influence of culinary treatments (boiling, microwaving, grilling, and deep frying) on proximate composition and antioxidant capacity of cultivated mushrooms (agaricus bisporus, lentinula edodes, pleurotus ostreatus, and pleurotus eryngii) was studied. proximate composition was affected by the cooking method and the mushrooms species. frying induced more severe losses in protein, ash, and carbohydrates content but increased the fat and energy. boiling improved the total glucans content by enhanci ...	2017	27760471
enhancement of the th1-phenotype immune system by the intake of oyster mushroom (tamogitake) extract in a double-blind, placebo-controlled study.	pleurotus cornucopiae (oyster mushroom, tamogitake) has long been eaten as a functional food for enhancement of the immune system, but its effectiveness has not been well confirmed in humans. to this end, we set up a double-blind placebo-controlled human clinical trial to investigate the potential of oyster mushrooms with respect to the up-regulation of the immune system. the subjects ingested oyster mushroom extract for 8 weeks. we measured the serum cytokine levels involved in regulation of th ...	2016	27774430
investigating the effect of tissue size on mycelial growth of seven mushroom species by using a novel device for precise tissue isolation.	tissue isolation from mushrooms is frequently practiced by both researchers and growers to isolate new and improved strains. in the present study, we designed a simple and convenient device for precise tissue isolation and therefore investigated the effect of tissue size on mycelial growth of seven mushroom species. the developed device consists of a cutting needle and a transfer needle. the cutting needle was used to obtain circular tissue plugs having a height up to 3 mm and variable diameters ...	2016	27784952
inhibition of oxidative stress and enhancement of cellular activity by mushroom lectins in arsenic induced carcinogenesis.	chronic arsenicosis is a major environmental health hazard throughout the world, including india. animals and human beings are affected due to drinking of arsenic contaminated ground water, due to natural mineral deposits, arsenical pesticides or improperly disposed arsenical chemicals. arsenic causes cancer with production of free radicals and reactive oxygen species (ros) that are neutralized by an elaborate antioxidant defense system consisting of enzymes and numerous non-enzymatic antioxidan ...	2016	27797216
comparative analysis of dna methyltransferase gene family in fungi: a focus on basidiomycota.	dna methylation plays a crucial role in the regulation of gene expression in eukaryotes. mushrooms belonging to the phylum basidiomycota are highly valued for both nutritional and pharmaceutical uses. a growing number of studies have demonstrated the significance of dna methylation in the development of plants and animals. however, our understanding of dna methylation in mushrooms is limited. in this study, we identified and conducted comprehensive analyses on dna methyltransferases (dnmtases) i ...	2016	27818666
evaluation of microwave-assisted and pressurized liquid extractions to obtain β-d-glucans from mushrooms.	microwave-assisted extraction (mae) and pressurized liquid extraction (ple) were compared as advanced technologies to obtain polysaccharides (particularly biologically active β-glucans) from pleurotus ostreatus and ganoderma lucidum fruiting bodies. extraction effectiveness was compared by a full-factorial experimental design (response surface methodology, rsm), using water as extraction solvent. total carbohydrate content of the obtained extracts and polysaccharide yields were the variable resp ...	2017	27842810
combinations of fungal and milling pretreatments for enhancing rice straw biogas production during solid-state anaerobic digestion.	rice straw was pretreated by different combinations of physical (milling) and biological (incubation with pleurotus ostreatus fungus) treatment to improve its biodegradability and biogas production during solid-state anaerobic digestion (ss-ad). effects of milling (⩽2mm) and incubation time (10, 20 and 30d), on lignin, cellulose, and hemicellulose degradation during fungal pretreatment and methane yield during digestion were assessed by comparison with untreated rice straw. both incubation time ...	2017	27864131
vitamin d2, ergosterol, and vitamin b2 content in commercially dried mushrooms marketed in china and increased vitamin d2 content following uv-c irradiation.	mushrooms are a great source of vitamin d and vitamin b2; however, the content of these vitamins in dried mushrooms has not fully been investigated. thus, the objectives of this study were to determine the contents of vitamin d2, ergosterol, and vitamin b2 in commercially dried edible mushrooms in china and to investigate the effect of uv-c irradiation on fresh mushrooms. among the 35 species of dried mushrooms considered for this study, the average ergosterol content was 1.98 mg/g, while the av ...	2016	27866467
identification of two mutations that cause defects in the ligninolytic system through an efficient forward genetics in the white-rot agaricomycete pleurotus ostreatus.	white-rot fungi play an important role in the global carbon cycle because they are the species that almost exclusively biodegrade wood lignin in nature. lignin peroxidases (lips), manganese peroxidases (mnps) and versatile peroxidases (vps) are considered key players in the ligninolytic system. apart from lips, mnps and vps, however, only few other factors involved in the ligninolytic system have been investigated using molecular genetics, implying the existence of unidentified elements. by comb ...	2017	27871142