Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [screening methodology for rice (oryza sativa) genotypes with high phosphorus use efficiency at their seedling stage]. | under hydroponic culture, the responses of rice (oryza sativa) genotypes to low p stress were respectively evaluated by means of relatively-higher p concentration in a controlled system with insoluble phosphate source (ca3 (po4)2) and of relatively-lower concentration with 0.5 mg p/l in which nah2po4 was the soluble phosphate. relative indices such as relative dry weight of tillers (rtw), total relative plant dry weight (rpw), relative number of tillers (rtn), relative dry weight of root (rrw), ... | 2002 | 12682961 |
| identification, cdna cloning and possible roles of seed-specific rice asparaginyl endopeptidase, rep-2. | we previously showed that two major cysteine endopeptidases, rep-1 and rep-2, were present in germinated rice ( oryza sativa l.) seeds, and that rep-1 was the enzyme that digests seed storage proteins. the present study shows that rep-2 is an asparaginyl endopeptidase that acts as an activator of rep-1, and we separated it into two forms, rep-2alpha (39 kda) and rep-2beta (40 kda), using ion-exchange chromatography and gel filtration chromatography. although analysis of the amino terminals revea ... | 2003 | 12684786 |
| control of tillering in rice. | tillering in rice (oryza sativa l.) is an important agronomic trait for grain production, and also a model system for the study of branching in monocotyledonous plants. rice tiller is a specialized grain-bearing branch that is formed on the unelongated basal internode and grows independently of the mother stem (culm) by means of its own adventitious roots. rice tillering occurs in a two-stage process: the formation of an axillary bud at each leaf axil and its subsequent outgrowth. although the m ... | 2003 | 12687001 |
| salinity tolerance of japonica and indica rice (oryza sativa l.) at the seedling stage. | in order to identify the degree of salinity tolerance of the indica and japonica rice groups, 10 varieties were tested under saline and non-saline conditions. twelve-day-old seedlings were grown in normal culture solution, then initially salinized at an electrical conductivity (ec) of 6 ds/m for 4 days, and finally salinized at an ec of 12 ds/m for the next 14 days. the growth parameters, and na and k absorption in the shoot were measured to characterize the tolerance level of the two rice group ... | 2003 | 12687373 |
| the evolution of constans-like gene families in barley, rice, and arabidopsis. | the co (constans) gene of arabidopsis has an important role in the regulation of flowering by photoperiod. co is part of a gene family with 17 members that are subdivided into three classes, termed group i to iii here. all members of the family have a cct (co, co-like, toc1) domain near the carboxy terminus. group i genes, which include co, have two zinc finger b-boxes near the amino terminus. group ii genes have one b-box, and group iii genes have one b-box and a second diverged zinc finger. an ... | 0 | 12692345 |
| male-sterility of thermosensitive genic male-sterile rice is associated with premature programmed cell death of the tapetum. | the tapetum plays a crucial role in pollen development. this secretory tissue produces numerous nutritive proteins necessary for pollen maturation. the tapetum, whose cells undergo programmed cell death (pcd), is completely diminished by the time the pollen is fully mature. our previous studies on a thermosensitive genic male-sterile (tgms) rice (oryza sativa l.) suggested that male-sterility was due to failure in pollen development. in this paper we describe how further analysis of the tgms ric ... | 2003 | 12692728 |
| "chitin-specific" peroxidases in plants. | the activity of various plant peroxidases and the ability of their individual isoforms to bind chitin was studied. some increase in peroxidase activity was observed in crude extracts in the presence of chitin. activated peroxidases of some species fell in the fraction not sorbed on chitin and those of other species can bind chitin. only anionic isoperoxidases from oat (avena sativa), rice (oryza sativa), horseradish (armoracia rusticana), garden radish (raphanus sativus var. radicula), peanut (a ... | 2003 | 12693984 |
| genomewide structural annotation and evolutionary analysis of the type i mads-box genes in plants. | the type i mads-box genes constitute a largely unexplored subfamily of the extensively studied mads-box gene family, well known for its role in flower development. genes of the type i mads-box subfamily possess the characteristic mads box but are distinguished from type ii mads-box genes by the absence of the keratin-like box. in this in silico study, we have structurally annotated all 47 members of the type i mads-box gene family in arabidopsis thaliana and exerted a thorough analysis of the c- ... | 2003 | 12698294 |
| digestion rate of legume carbohydrates and glycemic index of legume-based meals. | a study was performed to examine the rate of digestion of available carbohydrate in legumes and its mixtures with cereals, prepared as commonly eaten. the legumes and cereals studied were lentil (lens sculenta), pea (pisum sativum), bean (phaseolus vulgaris, var tortola), rice (oryza sativa) and spaghetti. foods were purchased at the city market. total starch content and the carbohydrate digestion rates were determined using the enzymatic method proposed by englyst et al. total starch levels ran ... | 2003 | 12701368 |
| characterization of selenate removal from drainage water using rice straw. | removal of selenium (se) from agricultural drainage water is very important for protecting wildlife in wetland systems. we conducted a series of experiments on selenite [se(iv)] adsorption and selenate [se(vi)] reduction to determine se removal from drainage water amended with 1000 microg/l of se(vi) or se(iv) and 5 g of rice (oryza sativa l.) straw. under sterile conditions, the added se(iv) was not adsorbed to the rice straw within 2 d of the experiment and the added se(vi) was not reduced wit ... | 2015 | 12708666 |
| rice uv-damaged dna binding protein homologues are most abundant in proliferating tissues. | ultraviolet-damaged dna binding protein (uv-ddb) is an important factor involved in dna repair. to study the role of uv-ddb, we attempted to obtain the cdna and the protein of a plant uv-ddb. we succeeded in isolating both genes for uv-ddb subunits from rice (oryza sativa cv. nipponbare), designated as osuv-ddb1 and osuv-ddb2. osuv-ddb2 (65 kda) was much larger than human uv-ddb2, but immunoprecipitation and gel mobility shift assay suggested that osuv-ddb2 is a plant counterpart of uv-ddb2. the ... | 2003 | 12711392 |
| linkage mapping of hsa-1(og), a resistance gene of african rice to the cyst nematode, heterodera sacchari. | inheritance of resistance to cyst nematode (heterodera sacchari) in oryza sativa was investigated by inoculation tests with isolate 244 from congo in segregating populations derived from hybridisation between o. sativa and its african sister cultivated species, o. glaberrima. we found that the resistance was controlled by one major gene, hsa-1(og), with codominance of susceptible and resistant alleles. to map hsa-1(og) on the rice genome, we pooled the data obtained from segregation of the resis ... | 2003 | 12721640 |
| characterization of rice functional monosaccharide transporter, osmst5. | cdna of a monosaccharide transporter in rice, osmst5 (oryza sativa monosaccharide transporter 5) was cloned and its sugar transport activity was characterized by heterologous expression analysis. the amino acid sequence and topology were similar to the sequences and topology of other plant monosaccharide transporters. yeast cells co-expressed with osmst5 cdna transported some monosaccharide substrates. the transport rate increased when ethanol as an electron donor was added, so the transporter w ... | 2003 | 12723603 |
| purification and characterization of extracellular beta-galactosidase secreted by supension cultured rice (oryza sativa l.) cells. | a beta-galactosidase was purified 1300-fold by lactosyl-sepharose 4b and sephacryl s-200 column chromatographies from the cultured medium of a rice-cell suspension. the purified enzyme appeared as 47 kd and 40 kd polypeptides on sds-page and had a specific activity of 65.1 units/mg. optimum activity was observed at ph 3.5 and 60 degrees c. the enzyme released galactose from galactoxyloglucan and pectic galactans. | 2003 | 12723614 |
| novel gene encoding a ca2+-binding protein and under hexokinase-dependent sugar regulation. | a cdna encoding a predicted 15-kda protein was earlier isolated from sugar-induced genes in rice embryos (oryza sativa l.) by cdna microarray analysis. here we report that this cdna encodes a novel ca2+-binding protein, named ossur1 (for oryza sativa sugar-up-regulated-1). the recombinant ossur1 protein expressed in escherichia coli had 45ca2+-binding activity. northern analysis showed that the ossur1 gene was expressed mainly in the internodes of mature plants and in embryos at an early stage o ... | 2003 | 12728997 |
| genetic manipulation of glycine decarboxylation. | the glycine-serine interconversion, catalysed by glycine decarboxylase and serine hydroxymethyltransferase, is an important reaction of primary metabolism in all organisms including plants, by providing one-carbon units for many biosynthetic reactions. in plants, in addition, it is an integral part of the photorespiratory metabolic pathway and produces large amounts of photorespiratory co(2) within mitochondria. although controversial, there is significant evidence that this process, by the relo ... | 2003 | 12730263 |
| the high diversity of snornas in plants: identification and comparative study of 120 snorna genes from oryza sativa. | using a powerful computer-assisted analysis strategy, a large-scale search of small nucleolar rna (snorna) genes in the recently released draft sequence of the rice genome was carried out. this analysis identified 120 different box c/d snorna genes with a total of 346 gene variants, which were predicted to guide 135 2'-o-ribose methylation sites in rice rrnas. though not exhaustive, this analysis has revealed that rice has the highest number of known box c/d snornas among eukaryotes. interesting ... | 2003 | 12736310 |
| cdna cloning of a rab5 homologue in babesia gibsoni. | for studying protein trafficking in babesia-infected erythrocyte, we describe the cloning of a rab5, one of molecular marker for vesicle trafficking in eukaryotic cells, gene homologue in babesia gibsoni (bgrab5). the full-length cdna of bgrab5 is 1,020 bp long with an open reading frame encoding a protein of 220 amino acids. the deduced amino acid sequence of bgrab5 contained the highly conserved gtp-binding consensus sequence and shares about 40% homology with that of rab5 from plasmodium falc ... | 2003 | 12736436 |
| mapping quantitative trait loci for yield, yield components and morphological traits in an advanced backcross population between oryza rufipogon and the oryza sativa cultivar jefferson. | an advanced backcross population between an accession of oryza rufipogon (irgc 105491) and the u.s. cultivar jefferson (oryza sativa ssp. japonica) was developed to identify quantitative trait loci (qtls) for yield, yield components and morphological traits. the genetic linkage map generated for this population consisted of 153 ssr and rflp markers with an average interval size of 10.3 cm. thirteen traits were examined, nine of which were measured in multiple environments. seventy-six qtls above ... | 2003 | 12736777 |
| expression of novel rice gibberellin 2-oxidase gene is under homeostatic regulation by biologically active gibberellins. | we have cloned two genes for gibberellin (ga) 2-oxidase from rice ( oryza sativa l.). expression of osga2ox2 was not observed. the other gene, osga2ox3, was expressed in every tissue examined and was enhanced by the application of biologically active ga. recombinant osga2ox3 protein catalyzed the metabolism of ga(1) to ga(8) and ga(20) to ga(29)-catabolite. these results indicate that osga2ox3 is involved in the homeostatic regulation of the endogenous level of biologically active ga in rice. | 2003 | 12736788 |
| isolation and functional characterization of the c-terminus of rice phosphatidylinositol 4-kinase in vitro. | a partial rice (oryza sativa l.) cdna clone, ospi4k1c, was isolated through screening of a cdna library constructed from tillering materials. ospi4k1c encoded a peptide of 608 amino acids with a calculated molecular mass of 68.4 kda. the ospi4k1c peptide shared high homology and possessed the highly conserved domains present in most isolated cloned pi4-kinases, i.e. a lipid kinase unique (lku) domain and a catalytic (cat) domain. a region with similarity to pleckstrin homology (ph) domain was pr ... | 2003 | 12737521 |
| cacta transposons in triticeae. a diverse family of high-copy repetitive elements. | in comparison with retrotransposons, which comprise the majority of the triticeae genomes, very few class 2 transposons have been described in these genomes. based on the recent discovery of a local accumulation of cacta elements at the glu-a3 loci in the two wheat species triticum monococcum and triticum durum, we performed a database search for additional such elements in triticeae spp. a combination of blast search and dot-plot analysis of publicly available triticeae sequences led to the ide ... | 2003 | 12746511 |
| towards an analysis of the rice mitochondrial proteome. | purified rice (oryza sativa) mitochondrial proteins have been arrayed by isoelectric focusing/polyacrylamide gel electrophoresis (page), by blue-native (bn) page, and by reverse-phase high-performance liquid chromatography (lc) separation (lc-mass spectrometry [ms]). from these protein arrays, we have identified a range of rice mitochondrial proteins, including hydrophilic/hydrophobic proteins (grand average of hydropathicity = -1.27 to +0.84), highly basic and acid proteins (isoelectric point = ... | 2003 | 12746528 |
| quantitative trait loci and comparative genomics of cereal cell wall composition. | quantitative trait loci (qtls) affecting sugar composition of the cell walls of maize (zea mays) pericarp were mapped as an approach to the identification of genes involved in cereal wall biosynthesis. mapping was performed using the ibm (b73 x mo17) recombinant inbred line population. there were statistically significant differences between b73 and mo17 in content of xylose (xyl), arabinose (ara), galactose (gal), and glucose. thirteen qtls were found, affecting the content of xyl (two qtls), a ... | 2003 | 12746531 |
| cloning and prokaryotic expression of a cdna encoding a putative mitochondrial malate dehydrogenase in oryza sativa. | malate dehydrogenase (mdh) has been characterized as a key player in oxaloacetate (oaa) biosynthesis mechanism in citrate acid cycle that generates reducing powers for further assimilation in the whole cell. here we present the cloning, characterization and prokaryotic expression of a putative mdh (osmmdh) in oryza sativa. sequence alignment shows that there is a high homology between the deduced amino acid sequence of osmmdh and mdh portein in eucalyptus gunnii (80%), as well as between the ded ... | 2003 | 12751334 |
| alien genes introgression and development of monosomic alien addition lines from oryza latifolia desv. to rice, oryza sativa l. | oryza latifolia, a tetraploid wild relative of cultivated rice is an important source of resistance to bacterial blight (bb), the brown planthopper (bph) and the whitebacked planthopper (wbph). interspecific hybrids were obtained between an elite breeding line (ir31917-45-3-2) of oryza sativa (2n=24 aa) and o. latifolia acc. no. 100914 (2n=48 ccdd). the crossability in f1 was 7.58% and it ranged from 0.11 to 0.62 in backcross generations. the f1 hybrid showed 2-6 ii, 0-2 iii, 0-1 iv and 22-32 i; ... | 2003 | 12764581 |
| a gene for a class ii dna photolyase from oryza sativa: cloning of the cdna by dilution-amplification. | ultraviolet radiation induces the formation of two classes of photoproducts in dna-the cyclobutane pyrimidine dimer (cpd) and the pyrimidine [6-4] pyrimidone photoproduct (6-4 product). many organisms produce enzymes, termed photolyases, which specifically bind to these lesions and split them via a uv-a/blue light-dependent mechanism, thereby reversing the damage. these photolyases are specific for either cpds or 6-4 products. two classes of photolyases (class i and class ii) repair cpds. a gene ... | 2003 | 12764611 |
| cloning and characterization of a glucose 6-phosphate/phosphate translocator from oryza sativa. | plastids of nongreen tissues import carbon as a source of biosynthetic pathways and energy, and glucose 6-phosphate is the preferred hexose phosphate taken up by nongreen plastids. a cdna clone encoding glucose 6-phosphate/phosphate translocator (gpt) was isolated from a cdna library of immature seeds of rice and named as osgpt. the cdna has one uninterrupted open reading frame encoding a 42 kda polypeptide possessing transit peptide consisting of 70 amino acid residues. the osgpt gene maps on c ... | 2015 | 12765288 |
| development of a quantitative pachytene chromosome map in oryza sativa by imaging methods. | a higher gc content region of an oryza sativa chromosome can be specifically visualized by double staining with propidium iodide (pi) and 4, 6-diamidino-2-phenylindole (dapi). this procedure allows identification of chromosome 9 from the other rice chromosomes at the pachytene stage. using rice chromosome 9 as a model, an imaging method to construct a pachytene chromosomal map was developed by quantifying the fluorescence profile (fp) of each chromomere. the pachytene map of chromosome 9 consist ... | 2003 | 12773815 |
| the promoter of a metallothionein-like gene from the tropical tree casuarina glauca is active in both annual dicotyledonous and monocotyledonous plants. | a chimeric gene consisting of the beta-glucuronidase (gusa) reporter gene under the control of the metallothionein-like promoter cgmt1 from the tropical tree casuarina glauca was introduced into nicotiana tabacum via agrobacterium tumefaciens and into oryza sativa by particle bombardment. the strongest histochemical staining for gus activity was observed in the root system of the transgenic plants, and especially in lateral roots. in contrast, a relatively low level of reporter gene expression w ... | 2003 | 12779116 |
| interaction between proliferating cell nuclear antigen and jun-activation-domain-binding protein 1 in the meristem of rice, oryza sativa l. | the eukaryotic polymerase processivity factor, proliferating cell nuclear antigen (pcna), interacts with many cell cycle-regulator proteins and with proteins involved in the mechanisms of dna replication and repair. in the present study using two-hybrid analysis with pcna from rice, oryza sativa l. cv. nipponbare (ospcna), we found that ospcna interacted in vitro and in vivo with rice jun-activation-domain-binding protein 1 (osjab1), which is known as cop9/signalsome subunit 5. both ospcna and o ... | 2003 | 12783325 |
| control of water uptake by rice ( oryza sativa l.): role of the outer part of the root. | a new pressure-perfusion technique was used to measure hydraulic and osmotic properties of the outer part of roots (opr) of 30-day-old rice plants (lowland cultivar: ir64, and upland cultivar: azucena). the opr comprised rhizodermis, exodermis, sclerenchyma and one cortical cell layer. the technique involved perfusion of aerenchyma of segments from two different root zones (20-50 mm and 50-100 mm from the tip) at precise rates using aerated nutrient solution. the hydraulic conductivity of the op ... | 2003 | 12783327 |
| structural and histochemical studies on grain-filling in the caryopsis of rice (oryza sativa l.). | the endosperm and embryo that constitute the filial tissues of rice caryopsis are isolated from the maternal tissues by the absence of any symplastic continuity. nutrients are transported to the endosperm through a single ovular vascular trace present on the ventral side of the ovary. initially solute enters through the chalaza into the nucellar projection and then into the endosperm. at later stages transport occurs through the nucellar epidermis, centripetally towards the endosperm. the cell w ... | 0 | 12799492 |
| role of abscisic acid in cadmium tolerance of rice (oryza sativa l.) seedlings. | changes in abscisic acid (aba) contents in cd-treated rice (oryza sativa l.) seedlings of two cultivars were investigated. on treatment with cdcl2, the aba content rapidly increased in the leaves and roots of cd-tolerant cultivar (cv. tainung 67, tng67) but not in the cd-sensitive cultivar (cv. taichung native 1, tn1). the reduction of transpiration rate of tn1 caused by cd was less than that of tng67. exogenous application of aba reduced transpiration rate, decreased cd content, and enhanced cd ... | 2003 | 12803614 |
| differential expression of three members of the amt1 gene family encoding putative high-affinity nh4+ transporters in roots of oryza sativa subspecies indica. | in order to investigate the molecular basis of high-affinity ammonium absorption by roots of rice plants (oryza sativa subspecies indica) the expression patterns of three members of the amt1 family of genes in rice seedling roots in response to altered nitrogen provision and diurnal changes in irradiance were examined. the 13nh4+ influx and transcript levels of osamt1.1 in roots decreased several fold within 48 h when plants acclimated to 10 micro m external nh4+ for 3 weeks were transferred to ... | 2003 | 12803618 |
| genomic comparison of p-type atpase ion pumps in arabidopsis and rice. | members of the p-type atpase ion pump superfamily are found in all three branches of life. forty-six p-type atpase genes were identified in arabidopsis, the largest number yet identified in any organism. the recent completion of two draft sequences of the rice (oryza sativa) genome allows for comparison of the full complement of p-type atpases in two different plant species. here, we identify a similar number (43) in rice, despite the rice genome being more than three times the size of arabidops ... | 2003 | 12805592 |
| identification and expression analysis of a gene encoding a bacterial-type phosphoenolpyruvate carboxylase from arabidopsis and rice. | phosphoenolpyruvate carboxylase (pepc) is distributed in plants and bacteria but is not found in fungi and animal cells. important motifs for enzyme activity and structure are conserved in plant and bacterial pepcs, with the exception of a phosphorylation domain present at the n terminus of all plant pepcs reported so far, which is absent in the bacterial enzymes. here, we describe a gene from arabidopsis, stated as atppc4, encoding a pepc, which shows more similarity to escherichia coli than to ... | 2003 | 12805623 |
| anoxia tolerance in rice roots acclimated by several different periods of hypoxia. | rice (oryza sativa l.) seedlings were subjected to hypoxic pretreatment (h-pt; incubated in 5% o2 atmosphere) for various lengths of time followed by an anoxic stress. anoxia tolerance of rice roots was improved with increasing duration of h-pt, but longer h-pt than 12 h gave no additional improvement. concentrations of atp and ethanol, and activities of pyruvate decarboxylase (ec 4.1.1.1) and alcohol dehydrogenase (ec 1.1.1.1) in the roots were increased by h-pt, and the times and patterns of i ... | 2003 | 12806786 |
| rice seedlings release momilactone b into the environment. | since the growth inhibitor momilactone b was found recently in root exudates of rice (oryza sativa l.), 3-day-old rice seedlings were transferred to hydroponic culture and the level of momilactone b released into the environment from the seedlings was measured. at day 15 after transfer, the level of momilactone b in the culture solution was 1.8 nmol per seedling compared with endogenous levels of 0.32 and 0.63 nmol per root and shoot, respectively, suggesting that rice seedlings actively release ... | 2003 | 12809715 |
| [a study on introduction of chitinase gene and beta-1,3-glucanase gene into restorer line of dian-type hybrid rice (oryza sativa l.) and enhanced resistance to blast (magnaporthe grisea)]. | plasmid pblgc containing chitinase gene from phaseolus limensis and beta-1,3-glucanase gene from nicotiana tabacum was bombarded into the restorer line "nan29" of dian-type hybrid rice (oryza sativa l. ssp. japonica) from yunnan province of south-west china. 93 regenerants were obtained from the calli that were resistant to g418 (100 to 150 mg/l) on nb medium. using beta-1,3 glucanase gene as the probe, 17 of the regenerants were identified to be transgenic lines by dot blotting and the foreign ... | 2003 | 12812057 |
| [synonymous codon usage bias in the rice cultivar 93-11 (oryza sativa l. ssp. indica)]. | by using the whole genome sequences and est data from the indica rice cultivar 93-11, a detailed relative analysis is made of the effect of some impact factors on synonymous codon usage. the results showed that the gene expression level assessed by mrna abundance is positive relative to the "codon adaptation index" (cai, 0.227**), and "codon preference parameter" (cpp, 0.145**), but negative relative to "effective number of codons" (enc, -0.147**), indicating that genes with higher expression sh ... | 2003 | 12812058 |
| qtl and epistatic interaction underlying leaf chlorophyll and h2o2 content variation in rice (oryza sativa l.). | it is meaningful to study the genetics of the traits associated with photosynthesis such as leaf chlorophyll and h2o2 contents for high yield breeding in rice. a mapping population of 98 bc1f9 lines (backcross inbred lines, bil), derived from a backcross of nipponbare (japonica)/kasalath(indica)//nipponbare by the single-seed-descent methods, was employed to map quantitative trait loci (qtl) underlying such rice traits as leaf chlorophyll and h2o2 contents. five and two qtls were detected for le ... | 2003 | 12812090 |
| mapping quantitative trait loci underlying appearance quality of rice grains (oryza sativa l.). | ninety-eight backcross inbred lines (bils) derived from a subspecific cross of nipponbare (japonica)/kasalath (indica)//nipponbare and its genetic linkage map were used to identify quantitative trait locus (qtl) controlling rice appearance quality traits such as grain length (gl), grain width (gw), length-width ratio (lwr), percentage of grains with chalkiness (pgwc), square of chalky endosperm (sce), degree of endosperm chalkiness (dec) and grain endosperm transparency (get) by composite interv ... | 2003 | 12812091 |
| osset1, a novel set-domain-containing gene from rice. | a novel set-domain-containing gene osset1 was isolated from rice (oryza sativa l.). its deduced protein consists of 895 amino acids. osset1 has a high degree of structure similarity to other set-domain-containing genes such as clf in higher plants and e(z) in animals. rt-pcr showed that the gene expresses throughout the entire plant. a transient expression assay in onion epidermis revealed that the osset1 protein is localized in nuclei. over-expression of the set domain of osset1 in arabidopsis ... | 2003 | 12815033 |
| family 19 chitinase from rice (oryza sativa l.): substrate-binding subsites demonstrated by kinetic and molecular modeling studies. | a family 19 chitinase (oschia1c, class i) from rice, oryza sativa l., and its chitin-binding domain-truncated mutant (oschia1c deltacbd, class ii) were produced by the pichia expression system, and the hydrolytic mechanism toward n-acetylglucosamine hexasaccharide [(glcnac)6] was investigated by hplc analysis of the reaction products. the profile of the time-course of (glcnac)6 degradation obtained by oschia1c was identical to that obtained by oschia1c deltacbd, indicating that the chitin-bindin ... | 2003 | 12825688 |
| a snapshot of the low temperature stress transcriptome of developing rice seedlings (oryza sativa l.) via ests from subtracted cdna library. | rice (oryza sativa l.) is sensitive to chilling particularly during early seedling development. given the biochemical complexity of tolerance mechanisms, genetic potential for this trait depends on highly coordinated expression of many genes. we used a simple cdna subtraction strategy to develop expressed sequence tags (ests) that represent an important subset of cold stress-upregulated genes. the 3,084 subtracted cdna clones represent a total of 1,967 unigenes from 1,354 singletons and 613 cont ... | 2003 | 12827255 |
| rice ubiquitin promoters: deletion analysis and potential usefulness in plant transformation systems. | strong constitutive promoters form a cornerstone for basic and applied research using transgenic plants. gus (beta-glucuronidase) expression levels from constructs containing rubq1 or rub2 rice ubiquitin promoters were 8- to 35-fold higher in transgenic rice [oryza sativa (l.)] plants, respectively, when compared to the 35s promoter. deletion analysis of the 5'-upstream region of rubq2 revealed a putative enhancer region that produced a 2.4-fold increase in transient gus expression. southern blo ... | 2003 | 12827439 |
| structure, heterologous expression, and properties of rice (oryza sativa l.) family 19 chitinases. | we identified four new family 19 chitinases in oryza sativa l. cv. nipponbare: one class i (oschia1d), two class ii (oschia2a and oschia2b), and one class iv (oschia4a). oschia2a resembled (about 60% identity) the catalytic domains of class i chitinases, but oschia2b was almost identical (95% identity) to that of the class iv enzyme. oschia1c, oschia1c deltacbd (a deletion of oschia1c lacking a chitin-binding domain, cbd), and oschia2b were separately expressed and purified in pichia pastoris. o ... | 2003 | 12834284 |
| improvement of rice (oryza sativa l.) seed oil quality through introduction of a soybean microsomal omega-3 fatty acid desaturase gene. | microsomal omega-3 fatty acid desaturase is an essential enzyme in the production of the n-3 polyunsaturated fatty acid alpha-linolenic acid during the seed developing stage. we have constructed a chimeric gene consisting of a maize ubi1-p-int and a soybean gmfad3 cdna, which was introduced into rice plants by agrobacterium-mediated transformation. ten transformants containing the chimeric gene were established and expression subsequently confirmed by northern blotting. furthermore, alpha-linole ... | 2003 | 12835909 |
| biochemical and molecular characterization of a rice glutelin allele for the glua-1 gene. | the rice ( oryza sativa l.) mutant of glu4a, lacking the glutelin alpha-2 subunit while the alpha-1 subunit increased (alpha-1h/alpha-2l), was used in this study. two-dimensional electrophoresis analysis revealed that the mutant lacked the polypeptide pi6.71/alpha-2 encoded by glu4 while forming a new polypeptide of pi6.50/alpha-1. experiments were conducted to identify the relationships between the mutated polypeptides of the mutant and to illustrate the mutation mechanism of the allele. peptid ... | 2003 | 12835929 |
| an ap2/erebp-type transcription-factor gene from rice is cold-inducible and encodes a nuclear-localized protein. | we cloned an ap2/erebp gene by dot blotting and named it osdrebl. analysis of its deduced amino-acid sequence indicated that this protein had a potential nuclear-localization signal, a possible acidic-activation domain and an ap2 dna binding domain. northern analysis showed that the transcripts of osdrebl accumulated rapidly (within 30 min) in response to low temperature, but not in response to aba, nacl and dehydration treatments. southern analysis indicated the presence of a single-copy of the ... | 2003 | 12844217 |
| genetic variations of aa genome oryza species measured by mite-aflp. | mites (miniature inverted-repeat transposable elements) are the major transposable elements in oryza species. we have applied the mite-aflp technique to study the genetic variation and species relationship in the aa-genome oryza species. high polymorphism was detected within and between species. the genetic variation in the cultivated species, oryza sativa and oryza glaberrima, was comparatively lower than in their ancestral wild species. in comparison between geographical lineages of the aa gen ... | 2003 | 12845436 |
| mapping qtls influencing rice floral morphology using recombinant inbred lines derived from a cross between oryza sativa l. and oryza rufipogon griff. | to understand the genetic basis of floral traits associated with the mating system in rice, we analyzed pistil, stamen and glume traits using a recombinant inbred line population, derived from a cross between an asian cultivated rice ( oryza sativa l.), pei-kuh, and a wild rice ( oryza rufipogon griff.), w1944. quantitative trait loci (qtls) affecting floral morphology were detected by composite interval mapping using a linkage map constructed using 147 markers, mostly rflps. a total of 7, 4, 14 ... | 2003 | 12845437 |
| production of doubled haploids through anther culture of m1 rice plants derived from mutagenized fertilized egg cells. | to produce stable mutants from mankeumbyeo, a japonica rice (oryza sativa l.) variety, we estimated the mutation efficiency of ethyl methane sulfonate (ems) and n-methyl-n-nitrosourea (mnu) on fertilized egg cells using doubled haploids (dhs) derived from anther culture of m1 plants. m1 seed production and germination were higher in 1 mm mnu than in 94.2 mm ems. a total of 68 dhs (35.4%) were regenerated by anther culture of m1 plants. twenty-one dhs (30.9%) were stable mutants, 14 dhs (20.6%) w ... | 2003 | 12845474 |
| putative functions of nucleoside diphosphate kinase in plants and fungi. | the putative functions of ndp (nucleoside diaphosphate) kinases from various organisms focusing to fungi and plants are described. the biochemical reactions catalyzed by ndp kinase are as follows. (i) phosphotransferring activity from mainly atp to cognate ndps generating nucleoside triphosphates (ntps). (ii) autophosphorylation activity from atp and gtp. (iii) protein kinase (phosphotransferring) activity phosphorylating such as myelin basic protein. ndp kinase could function to provide ntps as ... | 2003 | 12848342 |
| assessing hybrid sterility in oryza glaberrima x o. sativa hybrid progenies by pcr marker analysis and crossing with wide compatibility varieties. | interspecific crossing of the african indigenous rice oryza glaberrima with oryza sativa cultivars is hindered by crossing barriers causing 100% spikelet sterility in f(1) hybrids. since hybrids are partially female fertile, fertility can be restored by back crossing (bc) to a recurrent male parent. distinct genetic models on spikelet sterility have been developed predicting, e.g., the existence of a gamete eliminator and/or a pollen killer. linkage of sterility to the waxy starch synthase gene ... | 2003 | 12851767 |
| effect of irradiance on the partitioning of assimilated carbon during the early phase of grain filling in rice. | low irradiance in the early phase of grain filling in rice often results in a low grain yield, but its effects on the partitioning of previously or recently assimilated carbon within the plant or panicle have not been seriously examined. the objective of this study was to demonstrate the effect of shading during the different stages in the early phase of grain filling on the partitioning of previously or recently assimilated carbon among constituent organs and into superior and inferior spikelet ... | 2003 | 12853283 |
| hydraulic properties of rice and the response of gas exchange to water stress. | we investigated the role of xylem cavitation, plant hydraulic conductance, and root pressure in the response of rice (oryza sativa) gas exchange to water stress. in the field (philippines), the percentage loss of xylem conductivity (plc) from cavitation exceeded 60% in leaves even in watered controls. the plc versus leaf water potential relationship indicated diurnal refilling of cavitated xylem. the leaf water potential causing 50 plc (p(50)) was -1.6 mpa and did not differ between upland versu ... | 2003 | 12857848 |
| method development for the determination of iron in milligram amounts of rice plants (oryza sativa l.) from cultivation experiments using graphite furnace atomic absorption spectrometry. | the amount of sample that is available for analysis in laboratory plant cultivation experiments is usually very limited. highly sensitive analytical techniques are therefore required, even for elements that are present in the plants at mg g(-1) concentrations, and graphite furnace atomic absorption spectrometry (gfaas) was chosen in this work because of its micro-sampling capability, and its relatively simple operation. four micro-methods were investigated for the determination of iron in roots ... | 2003 | 12861434 |
| correlations between cadmium and mineral nutrients in absorption and accumulation in various genotypes of rice under cadmium stress. | the absorption and accumulation of cd2+, fe3+, zn2+, mn2+, cu2+ and mg2+ in the roots and leaves of 20 rice cultivars (oryza sativa l.) with different genotypes under cadmium (cd) stress were investigated with pot experiments. the results showed that there existed significant differences among the rice cultivars in the contents of six mineral elements in both roots and leaves at both heading and ripening periods. the statistical analysis showed that, for their contents in roots, significant and ... | 2003 | 12867177 |
| collection, mapping, and annotation of over 28,000 cdna clones from japonica rice. | we collected and completely sequenced 28,469 full-length complementary dna clones from oryza sativa l. ssp. japonica cv. nipponbare. through homology searches of publicly available sequence data, we assigned tentative protein functions to 21,596 clones (75.86%). mapping of the cdna clones to genomic dna revealed that there are 19,000 to 20,500 transcription units in the rice genome. protein informatics analysis against the interpro database revealed the existence of proteins presented in rice bu ... | 2003 | 12869764 |
| high-throughput functional affinity purification of mannose binding proteins from oryza sativa. | we have used affinity chromatography in combination with mass spectrometry to isolate, identify, and assign a preliminary functional annotation to a large number of both known and novel proteins from rice. rice (oryza sativa) leaf, root, and seed tissue extracts were fractionated by column affinity chromatography using alpha-d-mannose as the ligand. bound fractions were eluted and subjected to one-dimensional electrophoresis, followed by high-performance liquid chromatography-tandem mass spectro ... | 2003 | 12872227 |
| a new mite family, pangrangja, in gramineae species. | the miniature inverted-repeat transposable element (mite) is a type of class ii transposon. we have isolated a new mite, pangrangja, from the species oryza. although pangrangja elements are present in a number of gramineae species, they are more prevalent in a genome oryza species, triticum, aegilops, hordeum, and tripsacum. the pangrangja has a 16 bp terminal inverted repeat (tir) and conserved trinucleotides 5'-ttg-3' and 5'-aaa-3' at both ends. the tir starts with 5'-cagt-3', similar to the m ... | 2003 | 12872996 |
| genome-wide comparative phylogenetic analysis of the rice and arabidopsis dof gene families. | dof proteins are a family of plant-specific transcription factors that contain a particular class of zinc-finger dna-binding domain. members of this family have been found to play diverse roles in gene regulation of processes restricted to the plants. the completed genome sequences of rice and arabidopsis constitute a valuable resource for comparative genomic analyses, since they are representatives of the two major evolutionary lineages within the angiosperms. in this framework, the identificat ... | 2003 | 12877745 |
| 1h, 13c and 15n resonance assignments of rice telomere binding domain from oryza sativa. | 2003 | 12878845 | |
| distinct expression and function of three ammonium transporter genes (osamt1;1-1;3) in rice. | to study the regulation of ammonium uptake into rice roots, three ammonium transporter genes (osamt1;1, 1;2 and 1;3; oryza sativa ammonium transporter) were isolated and examined. osamt1s belong to amt1 family, containing 11 putative transmembrane-spanning domains. southern blot analysis and screening of the rice genome database confirmed that with osamt1;1-1;3 the complete amt1 family of rice had been isolated. heterologous expression of osamt1s in the yeast saccharomyces cerevisiae mutant 3101 ... | 2003 | 12881500 |
| cloning, characterization and functional expression of a new beta-d-fructofuranosidase (os beta fruct2) cdna from oryza sativa. | a new cdna (os beta fruct2) encoding an acid beta-d-fructofuranosidase from rice has been cloned, sequenced and expressed in pichia pastoris. the full-length cdna is 2453 base pairs long and encodes a pre-pro-protein of 682 amino acids. the cdna fragment coding for mature enzyme was sub-cloned into vector ppiczalphaa for extracellular expression in the methylotrophic yeast. the recombinant product was purified by ni2+-nitrilotriacetic acid agarose column and biochemically characterized. the enzy ... | 2003 | 12882270 |
| growth promotion and an increase in cell wall extensibility by silicon in rice and some other poaceae seedlings. | the effect of silicon on organ growth and its mechanisms of action were studied in rice ( oryza sativa l. cv. koshihikari), oat ( avena sativa l. cv. victory), and wheat ( triticum aestivum l. cv. daichino-minori) seedlings grown in the dark. applying silicon in the form of silicic acid to these seedlings via culture solution resulted in growth promotion of third (rice) or second (oat and wheat) leaves. the optimal concentration of silicon was 5-10 mm. no growth promotion was observed in early o ... | 2002 | 12884045 |
| soil properties and crop productivity as influenced by flyash incorporation in soil. | field experiments were carried out during 1996-97 at gulawathi, muthiani and salarpur villages, iari farm, new delhi and ncpp campus, dadri to evaluate changes in soil characteristics and growth of wheat (triticum aestivum l.), mustard (brassica juncea l.), lentil (lence esculenta moench.), rice (oryza sativa l.) and maize (zea mays l.) by varying amounts of flyash addition (up to 50t ha(-1)) in soils at sowing/transplanting time of crops. flyash addition in areas adjoining ncpp thermal power pl ... | 2003 | 12889638 |
| inheritance of oryza sativa endornavirus in f1 and f2 hybrids between japonica and indica rice. | we have found a 14 kbp double-stranded rna (dsrna) in many cultivars of japonica rice (oryza sativa l.) but not in any cultivars of indica rice. this dsrna is an rna replicon with plasmid-like properties and is proposed to be a novel dsrna virus, oryza sativa endornavirus (osv). reciprocal crosses between the osv-carrier japonica variety (nipponbare) and the osv-free indica variety (ir 26 or kasalath) were performed to investigate whether osv can be transmitted to f1 hybrids. when ir 26 and nipp ... | 2003 | 12893964 |
| mapping of quantitative trait loci associated with ultraviolet-b resistance in rice (oryza sativa l.). | the detection of quantitative trait loci (qtls) associated with uv-b resistance in rice should allow their practical application in breeding for such a complex trait, and may lead to the identification of gene characteristics and functions. considerable variation in uv-b resistance exists within cultivated rice (oryza sativa l.), but its detailed genetic control mechanism has not been well elucidated. we detected putative qtls associated with the resistance to enhanced uv-b radiation in rice, us ... | 2003 | 12898021 |
| reduction in the endogenous arginine decarboxylase transcript levels in rice leads to depletion of the putrescine and spermidine pools with no concomitant changes in the expression of downstream genes in the polyamine biosynthetic pathway. | we investigated whether down-regulation of arginine decarboxylase (adc) activity and concomitant changes in polyamine levels result in changes in the expression of downstream genes in the polyamine pathway. we generated transgenic rice (oryza sativa l.) plants in which the rice adc gene was down-regulated by expression of its antisense oat (avena sativa l.) ortholog. plants expressed the oat mrna adc transcript at different levels. the endogenous transcript was down-regulated in five out of eigh ... | 2003 | 12898254 |
| comparative genetics at the gene and chromosome levels between rice ( oryza sativa) and wildrice ( zizania palustris). | using comparative genetics, genes, repetitive dna sequences and chromosomes were studied in the oryzeae in order to more fully exploit the rice genome sequence data. of particular focus was zizania palustris l., n = 15, commonly known as american wildrice. previous work has shown that rice chromosomes 1, 4 and 9 are duplicated in wildrice. the adh1 and adh2 genes were sequenced and, based on phylogenetic analyses, found to be duplicated in wildrice. the majority of the sequence diversity in the ... | 2003 | 12904864 |
| alteration of floral organ identity in rice through ectopic expression of osmads16. | we used a transgenic approach and yeast two-hybrid experiments to study the role of the rice ( oryza sativa l.) b-function mads-box gene, osmads16. transgenic rice plants were generated that ectopically expressed osmads16 under the control of the maize ( zea mays l.) ubiquitin1 promoter. microscopic observations revealed that the innermost-whorl carpels had been replaced by stamen-like organs, which resembled the flowers of the previously described arabidopsis thaliana (l.) heynh. mutation super ... | 2003 | 12905025 |
| a tobacco (nicotiana tabaccum) calmodulin-binding protein kinase, ntcbk2, is regulated differentially by calmodulin isoforms. | a calcium (ca2+)/calmodulin (cam)-binding protein kinase (cbk) from tobacco (nicotiana tabaccum ), ntcbk2, has been characterized molecularly and biochemically. ntcbk2 has all 11 conserved subdomains of the kinase-catalytic domain and a cam-binding site as shown by other kinases, including ca2+-dependent protein kinase and chimaeric ca2+/cam-dependent protein kinases. however, this kinase does not contain an ef-hand motif for ca2+ binding, and its activity was not regulated by ca2+. whereas ntcb ... | 2003 | 12911329 |
| hyperphosphorylation of a mitochondrial protein, prohibitin, is induced by calyculin a in a rice lesion-mimic mutant cdr1. | the rice (oryza sativa) lesion-mimic mutants, cell death and resistance (cdr), show spontaneous cell death on the entire leaf and exhibited significant resistance to the rice blast fungus. our previous studies showed that cdr1 and cdr2 genes negatively regulated the phosphorylation steps leading to the activation of nadph oxidase, which is associated with oxidative burst. to identify novel factors involved in the phosphorylation steps, the phosphorylation level of total proteins was compared bet ... | 2003 | 12913143 |
| bwmk1, a rice mitogen-activated protein kinase, locates in the nucleus and mediates pathogenesis-related gene expression by activation of a transcription factor. | mitogen-activated protein kinase (mapk) cascades are known to transduce plant defense signals, but the downstream components of the mapk have as yet not been elucidated. here, we report an mapk from rice (oryza sativa), bwmk1, and a transcription factor, oserebp1, phosphorylated by the kinase. the mapk carries a tdy phosphorylation motif instead of the more common tey motif in its kinase domain and has an unusually extended c-terminal domain that is essential to its kinase activity and transloca ... | 2003 | 12913152 |
| effect of the herbicides oxadiazon and oxyfluorfen on phosphates solubilizing microorganisms and their persistence in rice fields. | a field experiment has been conducted with two herbicides viz. oxadiazon [5-terbutyl-3-(2,4-dichloro-5-isopropoxyphenyl)-1,3,4-oxadiazol-2-one] and oxyfluorfen [2-chloro-1-(3-ethoxy-4-nitrophenyl)-4-(trifluoromethyl) benzene] at rates of 0.4 and 0.12 kg a.i. ha(-1), respectively, to investigate their effect on the growth and activities of phosphate solubilizing microorganisms in relation to availability of phosphorus as well as persistence of the herbicides in the rhizosphere soil of wetland ric ... | 2003 | 12919781 |
| molecular marker dissection of rice (oryza sativa l.) plant architecture under temperate and tropical climates. | rice ( oryza sativa l.) plants develop vertically with shoot elongation and horizontally with tillering. the purpose of this study was to identify and characterize genomic regions influencing the rice plant architecture by quantitative trait locus (qtl) analysis for the component traits: culm length (cl), panicle length (pnl), panicle number (pnn) and tiller number (tn). for this qtl analysis, 191 recombinant inbred lines (f(7)) derived from a cross of milyang 23 (m23) and akihikari (ak) were gr ... | 2003 | 12920520 |
| [rice allelopathy to barnyardgrass]. | 138 rice (oryza sativa) germplasms were identified to study the allelopathy to barnyardgrass (echinochloa crusgalli), using the relay seeding technique. the results showed that qingkun 2 from jiangxi province, xiayitiao from jiangsu province, jizaoxian from anhui province, ganzaoxian 37 from jiangxi province, shangnuo 1, ir68465-2-3-2 from irri, and shuiyuan 2 from korea had a strongly excellent inhibition effect on barnyardgrass. results from pot culture showed that gumei 2 and zhong 156 had a ... | 2003 | 12924130 |
| [detection and analysis of qtl for seed dormancy in rice (oryza sativa l.) using ril and cssl population]. | a recombinant inbred line (ril) population and two chromosome segment substitution line (cssl) population derived from the cross of asominori (japonica) and ir24 (indica) were used to detect qtl controlling seed dormancy. cssl1 were a series of ir24 chromosome segment substitution lines in asominori background, and cssl2 were a series of introgression lines of asominori in the background of ir24. three qtl were detected on chromosome 3, 6 and 9 in ril population, and individual qtl accounted for ... | 2003 | 12924160 |
| black rice (oryza sativa l. indica) pigmented fraction suppresses both reactive oxygen species and nitric oxide in chemical and biological model systems. | anthocyanins, present in fruits and vegetables as natural colorants, have been well characterized to possess bioactive properties. anthocyanin components extracted from black rice (oryza sativa l. indica) separated by gel filtration and identified using lc-ms were cyanidin 3-glucoside and peonidin 3-glucoside. a standardized extract of black rice pigmented fraction (bre) containing known proportions of cyanidin 3-glucoside and peonidin 3-glucoside exhibited marked antioxidant activities and free ... | 2003 | 12926869 |
| activation of hypersensitive response genes in the absence of pathogens in transgenic tobacco plants expressing a rice small gtpase. | transgenic tobacco ( nicotiana tabacum l.) plants constitutively expressing a rice ( oryza sativa l.) gene encoding a small gtpase, rgp1, showed marked resistance to tobacco mosaic virus (tmv) infection compared with the wild type [h. sano et al. (1994) proc natl acad sci usa 91:10556-10560]. in order to examine the gene expression profile, the temperature-shift method was adopted to hyper-activate the n-gene inducing the hypersensitive response (hr), and transcripts of 11 representative hr gene ... | 2003 | 12937984 |
| enhanced pesticide sorption by soils containing particulate matter from crop residue burns. | lack of proper techniques to isolate black carbon (bc) from soils has hindered the understanding of their roles in the sorption and environmental fate of organic contaminants in soils and sediments. the burning of crop residues may be the primary source of bc in agricultural soils. in this study, wheat (triticum aestivum l.) and rice (oryza sativa l.) residues were burned, and the resulting particulate matter (ashes) along with a soil were used to sorb diuron from water. calculations indicated t ... | 2003 | 12953876 |
| splicing-site recognition of rice (oryza sativa l.) dna sequences by support vector machines. | it was found that high accuracy splicing-site recognition of rice (oryza sativa l.) dna sequence is especially difficult. we described a new method for the splicing-site recognition of rice dna sequences. | 2015 | 12958717 |
| mapping of qtls associated with cold tolerance during the vegetative stage in rice. | low-temperature stress is an important factor affecting the growth and development of rice (oryza sativa l.) in temperate and high-elevation areas. cold stress may cause various seedling injuries, delayed heading and yield reduction due to spikelet sterility. in this study, 181 microsatellite marker loci were used to identify quantitative trait loci (qtls) associated with cold tolerance at the vegetative stage in 191 recombinant inbred lines (rils) derived from a cross of a cold-tolerant tempera ... | 2003 | 12966040 |
| [changes in the cadmium content of rice during the milling process]. | milled rice and bran were prepared from brown rice (oryza sativa japonica) using a commercial scale rice-mill to investigate the changes in cadmium (cd) content caused by the milling process. rice sample solution was prepared by microwave digestion using hno3 and hf, then analyzed by icp-ms. cd was measured at m/z 114 in interference correction with moo and sn. the digestion method was validated with 4 kinds of certified reference material of "rice flour". we found that the cd content was reduce ... | 2003 | 12968468 |
| three distinct rice cellulose synthase catalytic subunit genes required for cellulose synthesis in the secondary wall. | several brittle culm mutations of rice (oryza sativa) causing fragility of plant tissues have been identified genetically but not characterized at a molecular level. we show here that the genes responsible for three distinct brittle mutations of rice, induced by the insertion of the endogenous retrotransposon tos17, correspond to cesa (cellulose synthase catalytic subunit) genes, oscesa4, oscesa7 and oscesa9. three cesa genes were expressed in seedlings, culms, premature panicles, and roots but ... | 2003 | 12970476 |
| differential metal selectivity and gene expression of two zinc transporters from rice. | zinc is an essential mineral for a wide variety of physiological and biochemical processes. to understand zinc transport in cereals, we identified putative zinc transporters in gene databases. three full-length cdnas were identified and characterized from rice (oryza sativa). two of the cdnas partially complemented a yeast (saccharomyces cerevisiae) mutant deficient in zinc uptake at low concentrations. the two transporters showed many similarities in function but differed in ionic selectivity a ... | 2003 | 12970480 |
| golden indica and japonica rice lines amenable to deregulation. | as an important step toward free access and, thus, impact of goldenrice, a freedom-to-operate situation has been achieved for developing countries for the technology involved. specifically, to carry the invention beyond its initial "proof-of-concept" status in a japonica rice (oryza sativa) cultivar, we report here on two transformed elite indica varieties (ir64 and mtl250) plus one japonica variety taipei 309. indica varieties are predominantly consumed in the areas with vitamin a deficiency. t ... | 2003 | 12970483 |
| molecular cloning and functional analysis of a novel type of bowman-birk inhibitor gene family in rice. | bowman-birk inhibitor (bbi) genes encode serine protease inhibitors that have repetitive cysteine-rich domains with reactive sites for the trypsin or chymotrypsin family. we have identified seven bbi genes from japonica rice (oryza sativa subsp. japonica var teqing). all of the genes identified were found in a single cluster on the southern end of the long arm of rice chromosome 1. four of the seven bbi genes have two repetitive cysteine-rich domains, whereas one has a truncated domain with only ... | 2003 | 12972663 |
| [molecular responses of rice (oryza sativa l.) to the stress of lowly enhanced uv-b radiation]. | the approach of mrna differential display (dd) was used to analyze the gene differential expression of rice seedling subjected to lowly enhanced ultraviolet-b radiation. the results showed that of the eighteen differential fragments, fifteen ones were different in expressional levels, another one was depressed, and the other two, named as ruvb1 and ruvb2 respectively, which could be recovered and re-amplified, were specifically induced. sequence analysis for ruvb2 through internet blast searchin ... | 2003 | 12974001 |
| environmental stress-induced extracellular isoperoxidase rc3 from rice. | effects of various environmental stresses such as heavy metals, salts and low (high) temperature on the secretion of peroxidase isozyme into the medium were examined in rice (oryza sativa cv. nak-dong) suspension culture. the major extracellular peroxidases secreted into the medium by various stresses were cationic isoperoxidases. a far migrating cationic isoperoxidase rc3 was isolated from the medium after application of cacl2, the effective stimulator for peroxidase secretion. isolation of ext ... | 2003 | 12974407 |
| [behavior of interspecific hybrids of oryza sativa l. and o. glaberrina steud]. | 1953 | 13051899 | |
| peculiar physiological behavior in rice (oryza sativa). | 1954 | 13186837 | |
| differential radiosensitivity in rice (oryza sativa linn.) at the genotypic level. | 1961 | 13750639 | |
| [radiobiological research on oryza sativa. i. total effects of x-ray]. | 0 | 14426888 | |
| functional dissections between gamyb and dof transcription factors suggest a role for protein-protein associations in the gibberellin-mediated expression of the ramy1a gene in the rice aleurone. | in the germinated cereal aleurone layer, gibberellic acids (ga) induce expression of a number of genes encoding hydrolytic enzymes that participate in the mobilization of stored molecules. previous analyses suggest that the key events controlling the ga-regulated gene expression in the aleurone are formation of active transcription machinery referred to as the ga responsive complex, followed by recruiting gamyb. in general, bipartite promoter contexts composed of the ga-responsive element and th ... | 2003 | 14500792 |
| lumenp--a neural network predictor for protein localization in the thylakoid lumen. | we report the development of lumenp, a new neural network-based predictor for the identification of proteins targeted to the thylakoid lumen of plant chloroplasts and prediction of their cleavage sites. when used together with the previously developed targetp predictor, lumenp reaches a significantly better performance than what has been recorded for previous attempts at predicting thylakoid lumen location, mostly due to a lower false positive rate. the combination of targetp and lumenp predicts ... | 2003 | 14500894 |
| ds tagging of branched floretless 1 (bfl1) that mediates the transition from spikelet to floret meristem in rice (oryza sativa l). | the genetics of spikelet formation, a feature unique to grasses such as rice and maize, is yet to be fully understood, although a number of meristem and organ identity mutants have been isolated and investigated in arabidopsis and maize. using a two-element ac/ds transposon tagging system we have isolated a rice mutant, designated branched floretless 1 (bfl1) which is defective in the transition from spikelet meristem to floret meristem. | 2003 | 14503923 |