Publications

effect of feeding cassava and/or stylosanthes foliage on the performance of crossbred growing cattle.	the effect of feeding different levels of cassava foliage (manihot esculenta, crantz) and/or stylosanthes guianensis foliage on the growth and digestibility was studied using twenty eight 6-month-old crossbred growing cattle (50% local yellow cattle and 50% sindhi) (both bos indicus) weighing on average 114 kg at start. all animals were fed a basal diet consisting of urea treated rice straw (urtrs) fed ad libitum, 0.87 kg concentrate and 0.22 kg molasses on dry matter (dm) basis. the treatments ...	2010	19521793
ants as biological indicators of wayana amerindian land use in french guiana.	we examined the ecological impact of traditional land use by wayana amerindians in french guiana using ants as bio-indicators. ants were sampled through a rapid assessment method and the core results analyzed using kohonen's self-organizing maps (som). our sample sites included: (1) a wayana village; (2) a cassava plantation; (3) an abandoned cassava plantation; (4) a forest fragment near the village; (5) a riparian forest; and (6) a primary terra firma forest. the ant diversity decreases accord ...	2009	19523607
production of lactic acid and ethanol by rhizopus oryzae integrated with cassava pulp hydrolysis.	cassava pulp was hydrolyzed with acids or enzymes. a high glucose concentration (>100 g/l) was obtained from the hydrolysis with 1 n hcl at 121 degrees c, 15 min or with cellulase and amylases. while a high glucose yield (>0.85 g/g dry pulp) was obtained from the hydrolysis with hcl, enzymatic hydrolysis yielded only 0.4 g glucose/g dry pulp. these hydrolysates were used as the carbon source in fermentation by rhizopus oryzae nrrl395. r. oryzae could not grow in media containing the hydrolysates ...	2010	19533174
molecular characterization of the plant virus genus ourmiavirus and evidence of inter-kingdom reassortment of viral genome segments as its possible route of origin.	ourmia melon virus (oumv), epirus cherry virus (epcv) and cassava virus c (csvc) are three species placed in the genus ourmiavirus. we cloned and sequenced their rna genomes. the sizes of the three genomic rnas of oumv, the type member of the genus, were 2814, 1064 and 974 nt and each had one open reading frame. rna1 potentially encoded a 97.5 kda protein carrying the gdd motif typical of rna-dependent rna polymerases (rdrps). the putative rdrps of ourmiaviruses are distantly related to known vi ...	2009	19535502
ammonium acetate enhances solvent production by clostridium acetobutylicum ea 2018 using cassava as a fermentation medium.	cassava, due to its high starch content and low cost, is a promising candidate substrate for large-scale fermentation processes aimed at producing the solvents acetone, butanol and ethanol (abe). however, the solvent yield from the fermentation of cassava reaches only 60% of that achieved by fermenting corn. we have found that the addition of ammonium acetate (ch(3)coonh(4)) to the cassava medium significantly promotes solvent production from cassava fermented by clostridium acetobutylicum ea 20 ...	2009	19543929
a nested case-control study of female breast cancer in karunagappally cohort in kerala, india.	lifestyle factors related to breast cancer risk were examined in a case-control study nested in a cohort in karunagappally, kerala, south india. we sought interviews with all the residents in karunagappally with the population of 385,103 (191,149 males and 193,954 females) in the 1991 census and established a cohort of 359,619 (93% of the population in 1991) in 1990. for analysis 264 breast cancer cases with age > or = 20 years were selected from 438 breast cancer cases reported during the perio ...	2009	19548367
[degradation of cyanide and maturity in cassava processing wastes composting].	an investigation was carried out to approach the degradation of cyanide and maturity during the cassava processing wastes composting process. mixtures of cassava hull, cassava residues and pig manure were used in the experiment. parameters like temperature, ph, cyanide, cellulose, hemicellulose, lignin and c/n ratio were assessed during the composting process, the effect of composting process on the degradation of cyanide and maturity were evaluated. the results reveal that the content of cyanid ...	2009	19558134
toxic effects of prolonged administration of leaves of cassava (manihot esculenta crantz) to goats.	cassava (manihot esculenta crantz) is a major source of dietary energy for humans and domestic animals in many tropical countries. however, consumption of cassava is limited by its characteristic content of cyanogenic glycosides. the present work aimed to evaluate the toxic effects of ingestion of cassava leaves by goats for 30 consecutive days, and to compare the results with the toxic effects of cyanide in goats, which have been described previously. eight alpine cross-bred female goats were d ...	2010	19559583
effect of granule size on the interference colors of starch in polarized light.	a nikitin-berek compensator tilted at 5.5 degrees in a polarizing microscope was used to create a background second-order blue interference color against which starch granules were examined. a grating monochromator showed the first interference minimum of the background was at 590 nm. starch granules have a radial molecular structure. thus, some radii were in line with the axis of the compensator while others were across the compensator axis. where radial birefringence counteracted the backgroun ...	2009	19585308
antimicrobial, mechanical, and barrier properties of cassava starch-chitosan films incorporated with oregano essential oil.	the physicochemical and antimicrobial properties of starch-chitosan films incorporated with oregano essential oil (oeo) have been investigated. the antimicrobial effects of starch-chitosan-oeo films against bacillus cereus, escherichia coli, salmonella enteritidis, and staphylococcus aureus were determined by the disk inhibition zone method. the film mechanical properties, water vapor permeability (wvp), fourier transform infrared spectra (ftir), and thermograms (tga) were also determined. films ...	2009	19627142
cyanide removal from cassava mill wastewater using azotobactor vinelandii tistr 1094 with mixed microorganisms in activated sludge treatment system.	cassava mill wastewater has a high organic and cyanide content and is an important economic product of traditional and rural low technology agro-industry in many parts of the world. however, the wastewater is toxic and can pose serious threat to the environment and aquatic life in the receiving waters. the ability of azotobactor vinelandii tistr 1094, a n2-fixing bacterium, to grow and remove cyanide in cassava wastewater was evaluated. results revealed that the cells in the exponential phase re ...	2009	19632039
gourd and squash artifacts yield starch grains of feasting foods from preceramic peru.	in a study of residues from gourd and squash artifacts, we recovered starch grains from manioc (manihot esculenta), potato (solanum sp.), chili pepper (capsicum spp.), arrowroot (maranta arundinacea), and algarrobo (prosopis sp.) from feasting contexts at the buena vista site, a central peruvian preceramic site dating to approximately 2200 calendar years b.c. this study has implications for the study of plant food use wherever gourds or squashes are preserved, documents the earliest evidence for ...	2009	19633184
effects of cassava starch hydrolysate on cell growth and lipid accumulation of the heterotrophic microalgae chlorella protothecoides.	heterotrophic fermentation of microalgae has been shown to accumulate high amounts of microalgal lipids, which are regarded as one of the most promising feedstocks for sustainable biodiesel production. to increase the biomass and reduce the cost of microalgal culture, the purpose of this study was to evaluate the possibility of using cassava starch hydrolysate (csh) instead of glucose as carbon source for heterotrophic culture of chlorella protothecoides in flasks. first, the two-step enzymatic ...	2009	19633877
influence of starch steeping period on dimensionless disintegration values of a paracetamol tablet formulation.	in this study, tapioca starches obtained after different steeping periods, i.e. ts 24, ts 48 and ts 72, were used as disintegrants with corn starch bp as the standard disintegrant in a paracetamol tablet formulation. two dimensionless disintegration quantities, t(n) and t(c) were used in the determination of the influence that steeping period of starch disintegrant would have on the crushing strength friability-disintegration time ratio (csfr/dt). these quantities were used to assess the influen ...	2013	19645332
characterization of the cassava geminivirus transcription activation protein putative nuclear localization signal.	the geminivirus transcription activation protein (trap) localizes to the nucleus and contains a putative nuclear localization signal (nls) ((28)prrrr(32)) on the n-terminus. the role of individual residues of this putative nls on nuclear localization and symptom induction was investigated using trap of east african cassava mosaic cameroon virus (eacmcv). subcellular localization was conducted using the green fluorescent protein (gfp). results showed that the proline residue at position 28 (pro-2 ...	2009	19665038
do sri lankan meals help decrease blood glucose response?	the prevalence of diabetes mellitus (dm) has rapidly increased in asian countries including sri lanka during the past decade. scientific data on postprandial glycaemic influence of common meals is essential when formulating diets. objectives of this study were to analyse glycaemic indices (gi) of five common meals and effects of macronutrients, sources of carbohydrates, and physicochemical properties of starch on observed gi values.	2009	19670545
in vitro estimates of iron bioavailability in some kenyan complementary foods.	iron-deficiency anemia is by far the most widespread micronutrient deficiency disease in the world, affecting more than 2 billion people. although there are multiple causes of anemia, its high prevalence among children-especially in developing countries such as kenya-is attributed to an inadequate intake of dietary iron.	2009	19689093
genotype-phenotype correlation in 9 patients with tropical pancreatitis and identified gene mutations.	the etiopathogenesis of tropical chronic pancreatitis (tcp) remains unclear. malnutrition, dietary toxins like cyanogens in cassava and micronutrient deficiency are proposed factors. the description and characterization of genetic factors in tcp has added a new dimension to the understanding of pathogenesis of the disease. however, there is sparse data on the association of tcp with cystic fibrosis transmembrane conductance regulator (cftr) gene mutations. we report 8 patients of tcp with cftr g ...	2009	19696993
amylase production by saccharomycopsis fibuligera a11 in solid-state fermentation for hydrolysis of cassava starch.	the optimization of process parameters for high amylase production by saccharomycopsis fibuligera a11 in solid-state fermentation was carried out using central composite design. finally, the optimal parameters obtained with the response surface methodology (rsm) were moisture 610.0 ml/kg, inoculum 30.0 ml (od(600 nm) = 20.0)/kg, the amount ratio of wheat bran to rice husk 0.42, cassava starch concentration 20.0 g/kg, temperature 28 degrees c, and natural ph. under the optimized conditions, 4,296 ...	2010	19701612
amarelinha do amapá: a carotenoid-rich cassava cultivar.	eight cassava (manihot esculenta crantz) cultivars, of which four are indigenous to the amazon region, along with an interspecific hybrid, were assessed for their carotenoid content. they were propagated and are maintained in the living manihot collection at the university of brasília. the cultivar "amarelinha do amapá", which was collected from the state of amapá, was found to have a very high content of beta-carotene: 27 mg per 100 mg, which is more than 50-fold higher than in other cassava cu ...	2009	19731202
the adenovirus-mediated linamarase/linamarin suicide system: a potential strategy for the treatment of hepatocellular carcinoma.	suicide gene therapy, also known as gene-directed enzyme prodrug therapy, has the potential to provide a cure for hepatocellular carcinoma (hcc), which is a leading cause of cancer death worldwide. linamarase from cassava is an enzyme that can hydrolyze the innocuous substrate linamarin into cyanide, which can block the mitochondrial respiratory chain of cells. to investigate whether linamarase and linamarin can be used for hcc treatment, a recombinant adenovirus carrying linamarase cdna was con ...	2010	19733963
gene-based microsatellites for cassava (manihot esculenta crantz): prevalence, polymorphisms, and cross-taxa utility.	cassava (manihot esculenta crantz), a starchy root crop grown in tropical and subtropical climates, is the sixth most important crop in the world after wheat, rice, maize, potato and barley. the repertoire of simple sequence repeat (ssr) markers for cassava is limited and warrants a need for a larger number of polymorphic ssrs for germplasm characterization and breeding applications.	2009	19747391
production of acetone-butanol-ethanol (abe) in direct fermentation of cassava by clostridium saccharoperbutylacetonicum n1-4.	in this work, acetone-butanol-ethanol (abe) fermentation characteristics of cassava starch and cassava chips when using clostridium saccharoperbutylacetonicum n1-4 was presented. the obtained results in batch mode using a 1-l fermenter showed that c. saccharoperbutylacetonicum n1-4 was a hyperamylolytic strain and capable of producing solvents efficiently from cassava starch and cassava chips, which was comparable to when glucose was used. batch fermentation of cassava starch and cassava chips r ...	2010	19771401
comparative nutritive value of cassava leaf meal, soya beans, fish meal and casein in diets for growing pigs.	a trial was conducted to investigate energy and nutrient digestibility of cassava leaf meal (clm), casein (cas), fish meal (fm), full-fat soya bean (ffsb) and extracted soya bean meal (sbm). the basal diet containing cassava root meal (crm) and ffsb meal and the other five dietary treatments were evaluated with 12 crossbred castrated male pigs in a metabolism study covering three successive experimental periods between 40 and 60 kg body weight (bw). total tract digestibility of dry matter (dm), ...	2004	19774760
growth and nutritive value of cassava (manihot esculenta cranz.) are reduced when grown in elevated co.	global food security in a changing climate depends on both the nutritive value of staple crops as well as their yields. here, we examined the direct effect of atmospheric co(2) on cassava (manihot esculenta cranz., manioc), a staple for 750 million people worldwide. cassava is poor in nutrients and contains high levels of cyanogenic glycosides that break down to release toxic hydrogen cyanide when damaged. we grew cassava at three concentrations of co(2) (c(a): 360, 550 and 710 ppm) supplied tog ...	2009	19778371
[application of icp-ms to detection of mineral elements and heavy metals in cassava's byproducts].	cassava is a main cultivated tropical crop in china, its rich starch roots are often used to produce fuel ethanol in recent years, so it's a kind of hot biomass energy crops. but cassava's byproducts such as leaves, stems and peels are regarded as waste, and are not fully utilized. cassava's byproducts contain many nutrients, and can be used to process high value food products. the contents of mineral elements and heavy metals in cassava's byproducts were studied by icp-ms. the results showed th ...	2009	19798987
searching for new clues about the molecular cause of endomyocardial fibrosis by way of in silico proteomics and analytical chemistry.	endomyocardial fibrosis (emf) -is a chronic inflammatory disease of the heart with related pathology to that of late stage chaga's disease. indeed, both diseases are thought to result from auto-immune responses against myocardial tissue. as is the case that molecular mimicry between the acidic termini of trypanosoma cruzi ribosomal p0, p1 and p2beta (or simply tcp0, tcp1, and tcp2beta) proteins and myocardial tissue causes chaga's disease, excessive exposure to certain infections, toxins includi ...	2009	19823676
a case-control study of roles of diet in colorectal carcinoma in a south indian population.	the worldwide incidence of colorectal cancer has increased rapidly in the past few decades and dietary habits have been implicated in the cause. though the indian diet varies substantially from western diet, there have not been detailed studies on any association.	2009	19827870
internal phloem in an interspecific hybrid of cassava, an indicator of breeding value for drought resistance.	we examined the stem anatomy of manihot esculenta crantz and its hybrid with m. oligantha pax. cross-sections were stained with safranin-alcian blue. manihot esculenta and its hybrid were found to have the same vascular structure in bicollateral bundles with internal phloem, which has been associated with drought resistance in other plant groups. if this association is established for cassava, it would facilitate the selection of lines that are more adapted to arid regions. this is the first rep ...	2009	19866433
optimization of diagnostic rt-pcr protocols and sampling procedures for the reliable and cost-effective detection of cassava brown streak virus.	sampling procedures and diagnostic protocols were optimized for accurate diagnosis of cassava brown streak virus (cbsv) (genus ipomovirus, family potyviridae). a cetyl trimethyl ammonium bromide (ctab) method was optimized for sample preparation from infected cassava plants and compared with the rneasy plant mini kit (qiagen) for sensitivity, reproducibility and costs. cbsv was detectable readily in total rnas extracted using either method. the major difference between the two methods was in the ...	2010	19879299
chapter 50: history of tropical neurology.	tropical neurology began less than two centuries ago. consumption of dietary toxins predominated at the beginning and gave birth to the geographic entity. the story moved from lathyrism through jamaican neuropathy to cassava-induced epidemic neuropathy, which was contrasted with konzo, also associated with cassava. other tropical diseases enumerated with chronological details include: chaga's diseases, kwashiorkor, madras type of motor neuron disease, atlanto-axial dislocation, burkitt's lymphom ...	2010	19892153
hydroxypropylated tapioca starch retards the development of insulin resistance in kkay mice, a type 2 diabetes model, fed a high-fat diet.	the hypoglycemic and antidiabetic effect of hydroxypropyl tapioca starch (hpts, degree of substitution = 0.180) was investigated in male kkay mice. mice were fed a purified high-fat (20%) diet without or with hpts (5% or 10%) for 33 d. gelatinized tapioca starch (ts) was used as a reference. fasting blood glucose concentrations, days 14 and 28, were significantly lower in the 10% hpts group compared with the reference. in an oral glucose tolerance test (ogtt), day 28, blood glucose concentration ...	2009	19895475
genotypic diversity of lactic acid bacteria isolated from african traditional alkaline-fermented foods.	to identify and compare lactic acid bacteria (lab) isolated from alkaline fermentations of cassava (manihot esculenta crantz) leaves, roselle (hibiscus sabdariffa) and african locust bean (parkia biglobosa) seeds for production of, respectively, ntoba mbodi, bikalga and soumbala.	2010	19895650
chemical composition of eucalyptus spp. essential oils and their insecticidal effects on lutzomyia longipalpis.	the chemical composition of essential oils from three species of plants belonging to the eucalyptus genus was determined and, their insecticidal effects on egg, larva and adult phases of lutzomyia longipalpis were assessed. the insects were collected in the municipality of sobral in the state of ceará, brazil. five treatments with different concentrations were performed along with two negative controls, distilled water and tween 80 (3%), and a positive control, cypermethrin (0.196mg/ml). the tes ...	2010	19896276
weissella beninensis sp. nov., a motile lactic acid bacterium from submerged cassava fermentations, and emended description of the genus weissella.	four gram-positive, catalase-negative, short rod-shaped or coccoid, heterofermentative lactic acid bacterial strains (2l24p13(t), 1l48p15, 1l24p31 and 1l24p34) with unusual phenotypic and genotypic properties were isolated from submerged fermenting cassava on mrs agar. all strains were motile, grew at 15 degrees c, produced dl-lactic acid from glucose with gas formation and produced ammonia from arginine. acid was produced from d-fructose, d-galactose, d-glucose, lactose, maltose, d-mannose, mel ...	2010	19897612
cacl2 enhanced somatic embryogenesis in manihot esculenta crantz.	primary cassava somatic embryos were induced on a medium without cacl(2), however, no or only a few secondary somatic embryos were formed from them. with 15 mm cacl(2) in the medium for induction of cassava primary embryos, more secondary somatic embryos were produced from them, and they were much effective in maintaining their embryogenic capacity than the controls of embryos which were induced without cacl(2).	2009	19897906
screening and production study of microbial xylanase producers from brazilian cerrado.	hemicelluloses are polysaccharides of low molecular weight containing 100 to 200 glycosidic residues. in plants, the xylans or the hemicelluloses are situated between the lignin and the collection of cellulose fibers underneath. the xylan is the most common hemicellulosic polysaccharide in cell walls of land plants, comprising a backbone of xylose residues linked by beta-1,4-glycosidic bonds. so, xylanolytic enzymes from microorganism have attracted a great deal of attention in the last decade, ...	2010	19898784
biodiversity and phytase capacity of yeasts isolated from tanzanian togwa.	the focus of the present investigation was on the tanzanian fermented food togwa as a source for dietary iron and zinc, and the potential for mineral availability improvements using selected yeasts. to establish the content of target minerals and main inhibitor for intestinal uptake, iron and zinc as well as the mineral chelating phytic acid, (ip(6) or phytate) were determined in naturally fermented togwa. yeasts were isolated from sorghum, maize and cassava based togwa, and identified by sequen ...	2010	19906458
nir determination of major constituents in tropical root and tuber crop flours.	tropical root and tuber crops (cassava, sweet potato, taro, and yam) are staples in developing countries where rapid urbanization is strengthening the demand for flour based foods. quality control techniques are still under development, and when available, laboratory analyses are too expensive. the objectives of this study were to calibrate near-infrared spectroscopy (nirs) for routine analysis of flours and to test its reliability to determine their major constituents. flours prepared from 472 ...	2009	19919112
yeast microflora isolated from brazilian cassava roots: taxonomical classification based on molecular identification.	a rapid molecular identification technique was applied on microbial microflora isolated from brazilian cassava roots given a yeast profile presented in the samples analyzed. a total of 24 strain isolated from cassava were initially grouped and identified in five groups using restriction-fragment length polymorphism (rflps) of 5.8s-its rdna region. sequencing analysis of the domains d1 and d2 of the 26s rrna gene or 5.8s rrna-its region were used to identify different groups of yeasts. representa ...	2010	19924478
the targets of acetone cyanohydrin neurotoxicity in the rat are not the ones expected in an animal model of konzo.	konzo is a neurotoxic motor disease caused by excess consumption of insufficiently processed cassava. cassava contains the cyanogenic glucoside linamarin, but konzo does not present the known pathological effects of cyanide. we hypothesized that the aglycone of linamarin, acetone cyanohydrin, may be the cause of konzo. this nitrile rapidly decomposes into cyanide and acetone, but the particular exposure and nutrition conditions involved in the emergence of konzo may favor its stabilization and s ...	2010	19932169
conservation and divergence of micrornas and their functions in euphorbiaceous plants.	micrornas (mirnas) are approximately 21 nt non-coding rnas which regulate post-transcriptional gene expression. mirnas are key regulators of nearly all essential biological processes. aiming at understanding mirna's functions in euphorbiaceae, a large flowering plant family, we performed a genome-scale systematic study of mirnas in euphorbiaceae, by combining computational prediction and experimental analysis to overcome the difficulty of lack of genomes for most euphorbiaceous species. specific ...	2010	19942686
evaluation of elastic modulus and hardness of crop stalks cell walls by nano-indentation.	agricultural biomaterials such as crop stalks are natural sources of cellulosic fiber and have great potential as reinforced materials in bio-composites. in order to evaluate their potential as materials for reinforcement, the nano-mechanical properties of crop-stalk cell walls, i.e. those of cotton (gossypium herbaceu) stalk, soybean (glycine max) stalk, cassava (manihot esculent) stalk, rice (oryza sativa l.) straw, and wheat (triticum aestivum l.) straw, were investigated by means of nano-ind ...	2010	19954968
studies on the paratyphoid - enteritidis group : vii. enteric infection ("food poisoning") due to tapioca pudding contaminated with b. cholerae suis (b. suipestifer).		1922	19972563
agrobacterium-mediated transformation of friable embryogenic calli and regeneration of transgenic cassava.	agrobacterium-mediated transformation of friable embryogenic calli (fec) is the most widely used method to generate transgenic cassava plants. however, this approach has proven to be time-consuming and can lead to changes in the morphology and quality of fec, influencing regeneration capacity and plant health. here we present a comprehensive, reliable and improved protocol, taking approximately 6 months, that optimizes agrobacterium-mediated transformation of fec from cassava model cultivar tms6 ...	2009	20010938
analysis of cassava brown streak viruses reveals the presence of distinct virus species causing cassava brown streak disease in east africa.	cassava brown streak virus (cbsv) isolates were analysed from symptomatic cassava collected between 1997 and 2008 in the major cultivation regions of east africa. an analysis of complete rna genomes of seven isolates from kenya, tanzania, mozambique, uganda and malawi revealed a common genome structure, but the isolates clearly clustered in two distinct clades. the first comprised isolates from kenya, uganda, malawi, north-western tanzania and the cbsv described previously, and shared between 87 ...	2010	20071490
tapioca starch graft copolymers and dome matrix modules assembling technology. i. effect of module shape on drug release.	this paper studies the riboflavin release from compressed disc modules of dome matrix(r) technology using tapioca starch-ethylmethacrylate (tsema) and tapioca hydroxypropylstarch-ethylmethacrylate (thsema), graft copolymers produced by two different drying methods. the comparison with the release behaviour of similar hpmc modules was performed. two different shape modules have been made, identified as female and male modules, in order to obtain their assemblage by interlocking the disc bases. hp ...	2010	20080180
flexible feeding on cultivated underground storage organs by rainforest-dwelling chimpanzees at bossou, west africa.	it has been proposed that exploitation of underground storage organs (usos) played an important role in the evolution of the genus homo, these items serving as 'fallback foods' during periods of low food availability. the use of usos as food by wild chimpanzees is infrequent and seen mostly in populations inhabiting relatively arid environments, such as the savanna. here, we specifically test the hypothesis that chimpanzees (pan troglodytes verus) inhabiting tropical wet forest at bossou (republ ...	2010	20080283
evaluation of the effect of four fibers on laxation, gastrointestinal tolerance and serum markers in healthy humans.	average dietary fiber intake in the united states is roughly half of the recommended amount. as new dietary fiber products are introduced to increase fiber intake, it is critical to evaluate the physiological effects of such fibers.	2010	20090313
characterization of hyperthermostable alpha-amylase from geobacillus sp. iiptn.	a newly isolated geobacillus sp. iiptn (mtcc 5319) from the hot spring of uttarakhand's himalayan region produced a hyperthermostable alpha-amylase. the microorganism was characterized by biochemical tests and 16s rrna gene sequencing. the optimal temperature and ph were 60 degrees c and 6.5, respectively, for growth and enzyme production. although it was able to grow in temperature ranges from 50 to 80 degrees c and ph 5.5-8.5. maximum enzyme production was in exponential phase with activity 13 ...	2010	20094713
the complete genome sequence of the tanzanian strain of cassava brown streak virus and comparison with the ugandan strain sequence.	the complete genome sequence for an isolate of the ugandan and tanzanian strain types of cassava brown streak virus have been determined using the novel approach of non-directed next generation sequencing. comparison of the genome sequences revealed that cbsv is highly heterogeneous at the isolate level as well as the strain level. the isolate of the ugandan strain was found to have a genome 9,070 nucleotides long coding for a polypeptide with 2,902 amino acid residues. the isolate of the tanzan ...	2010	20094895
[an item response theory analysis of the four-card selection task].	the four-card selection task (wason, 1966) is one of the best known tasks used in the literatures of human reasoning. this article analyzes this selection task by using item response theory (lord & novick, 1968). japanese undergraduates (n = 323) responded six types of the wason's selection tasks including indicative task (wason, 1966), beer task (griggs & cox, 1982), and cassava task (cosmides, 1989). an exploratory categorical factor analysis revealed a one factor structure of the six tasks. t ...	2009	20095447
acidification and starch behaviour during co-fermentation of cassava (manihot esculenta crantz) and soybean (glycine max merr) into gari, an african fermented food.	changes in acidification and starch behaviour were investigated during co-fermentation of cassava and soybean into gari, an african fermented product. non-volatile acidity, ph and starch content were evaluated using standard analytical methods. starch breakdown and pasting characteristics were also analysed using a brabender viscoamylograph. fermentation caused significant variations in the ph, non-volatile acidity and starch concentration. the ph decreased with concomitant increases in non-vola ...	2010	20109125
fermentative production of l(+)-lactic acid using hydrolyzed acorn starch, persimmon juice and wheat bran hydrolysate as nutrients.	the use of hydrolyzed acorn starch as a novel carbon source for l(+)-lactic acid production was proposed. the effects of carbon-nitrogen ratio and growth factor on the fermentations were studied by single factor experiments. a lower carbon-nitrogen ratio could enhance l(+)-lactic acid production, and the expensive yeast extract could be replaced by the cheap persimmon juice providing growth factor for l(+)-lactic acid production when wheat bran hydrolysate was used as the nitrogen source. the do ...	2010	20116239
an efficient treatment for detoxification process of cassava starch by plant cell wall-degrading enzymes.	the objective of this work was to remove linamarin in starch from cassava (manihot esculenta crantz cv. ku-50) roots, a high-cyanogen variety by using plant cell wall-degrading enzymes, xylanase and cellulase. the combination of xylanase from bacillus firmus k-1 and xylanase and cellulase from paenibacillus curdlanolyticus b-6 at the ratio of 1:9 showed the maximum synergism at 1.8 times for hydrolyzing cassava cortex cell walls and releasing linamarase. combined enzyme treatment enhanced linama ...	2010	20129074
briquetting of charcoal from sugar-cane bagasse fly ash (scbfa) as an alternative fuel.	brazil is the largest worldwide producer of alcohol and sugar from sugar-cane and has an extensive alternative program for car fuel which is unique. the objective of this work is to offer one management option of a solid residue produced by this industrial segment. the pressed sugar-cane bagasse is burned to produce steam and electricity by cogeneration. the combustion yields both bottom and fly ashes which contain high amounts of silicon oxide as a major component. fly ash which contains a high ...	2010	20133118
a reliable and efficient method for total rna isolation from various members of spurge family (euphorbiaceae).	it is prerequisite and crucial to extract rna with high quality and integrity in order to carry out molecular biology studies in any plant species of a family. euphorbiaceae members are known for high levels of their waxes, oils with polysaccharides, polyphenolics and secondary metabolites. these conditions are recognised to interfere unfavourably with various methodologies of rna isolation.	2012	20135710
constituents and secondary metabolite natural products in fresh and deteriorated cassava roots.	a phytochemical analysis of cassava (manihot esculenta crantz) fresh roots and roots suffering from post-harvest physiological deterioration (ppd) has been carried out. the first isolation and identification of galactosyl diacylglycerides from fresh cassava roots is reported, as well as beta-carotene, linamarin, and beta-sitosterol glucopyranoside. the hydroxycoumarin scopoletin and its glucoside scopolin were identified from cassava roots during ppd, as well as trace quantities of esculetin and ...	2010	20137795
antimicrobial activities of tapioca starch/decolorized hsian-tsao leaf gum coatings containing green tea extracts in fruit-based salads, romaine hearts and pork slices.	the antimicrobial activities of edible coatings based on a tapioca starch/decolorized hsian-tsao leaf gum (dhg) matrix with various green tea extracts (gtes) were evaluated. its effect on the shelf-life extension of fruit-based salads, romaine hearts, and pork slices were investigated as well. three types of gtes from hot water (80 degrees c) (w), 40% (e4) and 80% (e8) ethanol were prepared. it was found that all gtes showed pronounced inhibition on gram positive bacteria in agar media, includin ...	2010	20138382
conversion of cassava wastes for biofertilizer production using phosphate solubilizing fungi.	two fungi characterized as aspergillus fumigatus and aspergillus niger, isolated from decaying cassava peels were used to convert cassava wastes by the semi-solid fermentation technique to phosphate biofertilizer. the isolates solubilized ca(3)(po(4))(2), alpo(4) and fepo(4) in liquid pikovskaya medium, a process that was accompanied by acid production. medium for the ssf fermentation was composed of 1% raw cassava starch and 3% poultry droppings as nutrients and 96% ground (0.5-1.5mm) dried cas ...	2010	20138509
lactic acid production from cassava fibrous residue using lactobacillus plantarum mtcc 1407.	during extraction of starch from cassava, fibrous residue is a major waste released into the environment. owing to the high starch content (60-65% on dry weight basis) and organic matter of cassava fibrous residue (cfr), an attempt has been made to utilize it for the production of lactic acid (la) in semi solid state fermentation using mann rogassa sharpe medium containing [5 % (wv(-1))] cfr in lieu of glucose [2% (wv(-1))] as the carbon source. response surface methodology (rsm) was used to eva ...	2009	20143717
developmenrt of est-ssr and genomic-ssr markers to assess genetic diversity in jatropha curcas l.	abstract:	2010	20181259
consuming cassava as a staple food places children 2-5 years old at risk for inadequate protein intake, an observational study in kenya and nigeria.	inadequate protein intake is known to be deleterious in animals. using who consensus documents for human nutrient requirements, the protein:energy ratio (p:e) of an adequate diet is > 5%. cassava has a very low protein content. this study tested the hypothesis that nigerian and kenyan children consuming cassava as their staple food are at greater risk for inadequate dietary protein intake than those children who consume less cassava.	2010	20187960
proteome characterization of cassava (manihot esculenta crantz) somatic embryos, plantlets and tuberous roots.	proteomics is increasingly becoming an important tool for the study of many different aspects of plant functions, such as investigating the molecular processes underlying in plant physiology, development, differentiation and their interaction with the environments. to investigate the cassava (manihot esculenta crantz) proteome, we extracted proteins from somatic embryos, plantlets and tuberous roots of cultivar sc8 and separated them by sodium dodecyl sulfate polyacrylamide gel electrophoresis ( ...	2010	20187967
the evolutionary ecology of clonally propagated domesticated plants.	while seed-propagated crops have contributed many evolutionary insights, evolutionary biologists have often neglected clonally propagated crops. we argue that widespread notions about their evolution under domestication are oversimplified, and that they offer rich material for evolutionary studies. the diversity of their wild ancestors, the diverse ecologies of the crop populations themselves, and the intricate mix of selection pressures, acting not only on the parts harvested but also on the pa ...	2010	20202131
a new strain of indian cassava mosaic virus causes a mosaic disease in the biodiesel crop jatropha curcas.	jatropha curcas mosaic disease is a newly emerging disease that challenges the productivity of a prospective biofuel crop, j. curcas. the aetiology of this disease has not been resolved. here, we report the complete nucleotide sequences of a jatropha virus isolated from dharwad, southern india. phylogenetic analysis of the virus genome suggests it is a new strain of indian cassava mosaic virus. agroinfiltration of the two cloned viral dna components produced systemic infection and typical mosaic ...	2010	20224893
improved yields of cyclic nigerosylnigerose from starch by pretreatment with a thermostable branching enzyme.	cyclic nigerosylnigerose (cnn) is produced enzymatically from starch by the combined action of 6-alpha-glucosyltransferase and 3-alpha-isomaltosyltransferase. in our previous study, alpha-1,6-branching chains found in the structure of amylopectin and glycogen were shown to be favorable for cnn formation by the two enzymes. therefore, we examined whether the introduction of alpha-1,6-branch points into starch using the action of branching enzyme (be) could improve the yield of cnn from starch. th ...	2010	20226381
small ruminant feed systems: perceptions and practices in the transitional zone of ghana.	adequate feeding is essential to realizing the potential of small ruminants to alleviate poverty among smallholder farmers. this study was conducted in two villages in the ejura-sekyedumase district of ghana and was motivated by farmers' non-adoption of modern feed technologies, but more importantly by the need to understand the small ruminant feed system considering farmers' different socio-economic backgrounds and how these relate to small ruminant performance. in this study, the feed system w ...	2010	20298617
rearing the cotton bollworm, helicoverpa armigera, on a tapioca-based artificial diet.	the impact of a tapioca-based artificial diet on the developmental rate, life history parameters, and fertility was examined over five consecutive generations for the cotton bollworm, helicoverpa armigera hubner (lepidoptera: noctuidae), a highly polyphagous pest of many agricultural crops. the study showed that when fed the tapioca-based artificial diet during larval stage, larval and pupal developmental period, percent pupating, pupal weight, emergence rate of male and female, longevity, fecun ...	2007	20302464
differential interaction between cassava mosaic geminiviruses and geminivirus satellites.	geminiviruses are often associated with subviral agents called dna satellites that require proteins encoded by the helper virus for their replication, movement and encapsidation. hitherto, most of the single-stranded dna satellites reported to be associated with members of the family geminiviridae have been associated with monopartite begomoviruses. cassava mosaic disease is known to be caused by viruses belonging to nine different begomovirus species in the african continent and the indian subc ...	2010	20335493
expression pattern conferred by a glutamic acid-rich protein gene promoter in field-grown transgenic cassava (manihot esculenta crantz).	a major constraint for incorporating new traits into cassava using biotechnology is the limited list of known/tested promoters that encourage the expression of transgenes in the cassava's starchy roots. based on a previous report on the glutamic-acid-rich protein pt2l4, indicating a preferential expression in roots, we cloned the corresponding gene including promoter sequence. a promoter fragment (cp2; 731 bp) was evaluated for its potential to regulate the expression of the reporter gene gusplu ...	2010	20336312
[enhancing ethanol production using thermophilic yeast by response surface methodology].	we optimized the conditions of simultaneous saccharification and fermentation (ssf) from cassava flour into high-concentration ethanol by thermophilic yeast gxasy-10. based on the single factor experiment, we screened the important parameters by plackeet-burman design. we used the path of steepest ascent to approach to the biggest region of ethanol production subsequently. then, we obtained the optimum values of the parameters by box-behnken design. the results showed that the important paramete ...	2010	20353091
short-duration cassava genotypes for crop diversification in the humid tropics: growth dynamics, biomass, yield and quality.	short-duration (6-7 months) cassava provides opportunities to smallholder farmers for effective utilisation of resources such as land, moisture and nutrients as well as diversification of enterprise and income. the variation in biomass production and partitioning, seasonal course of growth indices, yield, quality and nutrient uptake of ten short-duration/early-bulking genotypes of cassava and their impact on nutrient contents in soil in a lowland situation akin to rice fallow were examined in th ...	2010	20355030
a novel full recycling process through two-stage anaerobic treatment of distillery wastewater for bioethanol production from cassava.	in the present study, a novel full recycling process for bioethanol production was investigated, where three mathematical models were established to simulate the accumulation of major soluble inhibitory substances, including organic compounds, total ions, volatile fatty acids (vfas) and colorants. these inhibitory substances in the reused water reached a relative steady state after 3-7 batches of anaerobic treatment and recycling process, which coincided with the results of mathematical models. ...	2010	20363557
the effects of arbuscular mycorrhizal fungal inoculation and mulch of contrasting chemical composition on the yield of cassava under humid tropical conditions.	the influence of arbuscular mycorrhizal fungus (amf), glomus deserticola, and leaf mulch from gliricidia sepium and senna siamea on the yield of cassava (manihot esculenta) in a degraded alfisol of southwestern nigeria was investigated. inoculation in conjunction with mulching increased cassava tuber yield by 40-278% over the control. the highest yield was obtained with g. sepium and s. siamea mulch applied together in equal proportions. the results are explained in the light of the growth-enhan ...	2010	20364236
putative storage root specific promoters from cassava and yam: cloning and evaluation in transgenic carrots as a model system.	a prerequisite for biotechnological improvements of storage roots is the availability of tissue-specific promoters enabling high expression of transgenes. in this work, we cloned two genomic fragments, pme1 and pdj3s, controlling the expression of a gene with unknown function from cassava (manihot esculenta) and of the storage protein dioscorin 3 small subunit gene from yam (dioscorea japonica), respectively. using beta-glucuronidase as a reporter, the activities of pme1 and pdj3s were evaluated ...	2010	20369359
malaria knowledge and agricultural practices that promote mosquito breeding in two rural farming communities in oyo state, nigeria.	agricultural practices such as the use of irrigation during rice cultivation, the use of ponds for fish farming and the storage of water in tanks for livestock provide suitable breeding grounds for anthropophylic mosquitoes. the most common anthropophylic mosquito in nigeria which causes much of the morbidity and mortality associated with malaria is the anopheles mosquito. farmers are therefore at high risk of malaria - a disease which seriously impacts on agricultural productivity. unfortunatel ...	2010	20380703
pre-columbian agricultural landscapes, ecosystem engineers, and self-organized patchiness in amazonia.	the scale and nature of pre-columbian human impacts in amazonia are currently hotly debated. whereas pre-columbian people dramatically changed the distribution and abundance of species and habitats in some parts of amazonia, their impact in other parts is less clear. pioneer research asked whether their effects reached even further, changing how ecosystems function, but few in-depth studies have examined mechanisms underpinning the resilience of these modifications. combining archeology, archeob ...	2010	20385814
genetic, embryonic and anatomical study of an interspecific cassava hybrid.	a molecular, anatomical and cytogenetic study of an interspecific hybrid between manihot esculenta (cassava) and the wild species m. oligantha was carried out. cytogenetics revealed relatively complete chromosome pairing and high viability of the pollen grains. ovule structure examined by the clearing method showed polyembryony in 2.7% of the ovules. doubling of the chromosome number resulted in an increase in polyembryony of up to 18% and a reduction in pollen viability. multivalent formation w ...	2010	20391337
determination of dynamic characteristics of microbiota in a fermented beverage produced by brazilian amerindians using culture-dependent and culture-independent methods.	cauim is a fermented beverages prepared by tapirapé amerindians in brazil from substrates such as cassava, rice, peanuts, pumpkin, cotton seed and maize. here we study the microorganisms associated with peanut and rice fermentation using a combination of culture-dependent and -independent methods. the bacterial population varied from 7.4 to 8.4 log cfu/ml. the yeast population varied from 4.0 to 6.6 log cfu/ml. a total of 297 bacteria and yeasts strains were isolated during fermentation, with 19 ...	2010	20413168
influence of different substrates on the production of a mutant thermostable glucoamylase in submerged fermentation.	three mutations, ser54→pro, thr314→ala, and his415→tyr, were identified in aspergillus awamori glucoamylase gene expressed by saccharomyces cerevisiae. the mutant glucoamylase (ga) was substantially more thermostable than a wild-type ga at 70 °c, with a 3.0 kj mol(-1) increase in the free energy of thermo-inactivation. the effect of starch from different botanical sources on the production of this ga was measured in liquid fermentation using commercial soluble starch, cassava, potato, and corn a ...	2011	20414741
enhancer/suppressor mutator (en/spm)-like transposable elements of cassava (manihot esculenta) are transcriptionally inactive.	transposable elements contribute to the size, structure, variation, and diversity of the genome and have major effects on gene function. sequencing projects have revealed the diversity of transposable elements in many organisms and have shown that they constitute a high percentage of the genome. pcr-based techniques using degenerate primers designed from conserved enzyme domains of transposable elements can provide quick and extensive surveys, making study of diversity and abundance and their ap ...	2010	20449796
protein and starch concentrates of air-classified field pea and zero-tannin faba bean for weaned pigs.	air-classified pulse (non-oilseed legume) protein and starch may replace specialty protein and starch feedstuffs in diets for weaned pigs. in exp. 1, three specialty protein sources (5% soy protein concentrate, 5% corn gluten meal, and 5% menhaden meal in the control diet) were replaced with 16% zero-tannin hulled or dehulled faba bean, or 17.5% field pea protein concentrate. in total, 192 group-housed pigs (2 gilts and 2 barrows per pen; bw = 7.5 +/- 1.4 kg) were fed wheat-based diets (3.60 mca ...	2010	20453087
cassava root diet induces low pyruvate levels.	the high cyanogenic-glucoside carbohydrate of the cassava root (manihot esculenta) has special properties that make it an ideal therapeutic food for lowering nicotinamide adenine dinucleotide reduced form (nadh) and inducing sirtuin (sirt) gene overexpression when eaten in an exclusive mono-food diet regime. the author, using himself as the sole test subject, repeatedly induced low pyruvate levels (reflective of nadh levels) after being on the diet for 1-2 weeks at a time. the possible influence ...	2013	20462383
isolation and characterization of ethanol-producing schizosaccharomyces pombe chfy0201.	ethanol-producing yeast strain, chfy0201 was isolated from soil in south korea using an enrichment technique in a yeast peptone dextrose medium supplemented with 5% (w/v) ethanol at 30 degrees c. the phenotypic and physiological characteristics, as well as molecular phylogenetic analysis based on the d1/d2 domains of the large subunit (26s) rdna gene and the internally transcribed spacer (its) 1+2 regions suggested that the chfy0201 was novel strain of schizosaccharomyces pombe. during shaking f ...	2010	20467261
sequestering of cu(ii) from aqueous solution using cassava peel (manihot esculenta).	cassava peel is a prospective cheap biosorbent for metal ions sequestration. in this research, the ability of cassava peel to remove cu(ii) from aqueous solution was evaluated. its physical characteristics were probed by nitrogen adsorption measurements and scanning electron microscopy while its biosorption mechanism was studied by fourier transform infrared (ftir), scanning electron microscopy/energy dispersive using x-ray analysis-(sem/edx), x-ray mapping and x-ray photoelectron spectroscopy ( ...	2010	20471169
effective ethanol production by reutilizing waste distillage anaerobic digestion effluent in an integrated fermentation process coupled with both ethanol and methane fermentations.	an integrated ethanol-methane fermentation coupled system characterized with full wastewater reutilization was proposed. the waste distillage originated from ethanol distillation was treated with anaerobic digestion and then recycled for medium preparation in the next ethanol fermentation run. this process could enhance wastewater reutilization, save fresh water and reduce energy consumption in the cassava-based ethanol production. the results indicated that, when using anaerobic effluents from ...	2010	20473528
hiv and hepatocellular and esophageal carcinomas related to consumption of mycotoxin-prone foods in sub-saharan africa.	promotion of the hiv epidemic by aflatoxin is postulated but not yet established. sub-saharan populations commonly consume food contaminated by mycotoxins, particularly aflatoxins (predominantly found in peanut, maize, rice, and cassava) and fumonisins, which occur primarily in maize. aflatoxin promotes hepatocellular cancer, and fumonisin may promote esophageal cancer.	2010	20484447
effects of supplementing rice straw with leucaena (leucaena leucocephala) and madras thorn (pithecellobium dulce) foliages on digestibility, microbial n supply and nitrogen balance of growing goats.	a total of 12 crossbred (boer × anglo-nubian) goats were chosen from a commercial farm on the basis of similar body weight (25.0 ± 3.1 kg). the goats were housed in individual pens and allowed 3 weeks to adapt to experimental conditions. the goats were randomly allocated to three treatments in a 3 × 3 latin square experiment (replicated four times). within each period, each goat was given rice straw as roughage plus the respective treatment diet. the diets were iso-nitrogenous and iso-energetic ...	2010	20487095
evaluation of nondigested carbohydrates in hydroxypropylated tapioca starch.	in vitro and in vivo digestibilities of hydroxypropyl starch were investigated to determine an appropriate nondigested carbohydrate assaying method for hydroxypropyl starch. hydroxypropyl tapioca starch (hpts), with a 0.338 degree of substitution, was used as a hydroxypropyl starch source. practically all nondigested carbohydrate in hpts was low molecular weight and was not precipitated in 78% ethanol. the contents of nondigested carbohydrate in hpts and in effluents of ileorectomized rats fed t ...	2010	20492172
effect of different binders on the physico-chemical, textural, histological, and sensory qualities of retort pouched buffalo meat nuggets.	the functional properties of 4 binders, namely corn starch, wheat semolina, wheat flour, and tapioca starches, were evaluated to improve the quality of buffalo meat nuggets processed in retort pouches at f(0) 12.13. incorporation of corn starch in buffalo meat nuggets produced more stable emulsion than other binders used. product yield, drip loss, and ph did not vary significantly between the products with different binders. shear force value was significantly higher for product with corn starch ...	2016	20492199
feeding and performance of pigs in smallholder production systems in northern lao pdr.	a survey was carried out in the north of lao pdr to study feeding systems and the performance of pigs in smallholder systems. a total of 341 farmers from five provinces were interviewed. to feed their pigs, farmers mainly rely on feedstuffs produced on the farm and collected from areas near the village. the feedstuffs used included by-products such as rice bran and distiller's waste, planted feeds, mainly maize and cassava, and various green plant materials. the feedstuffs used are usually high ...	2010	20517643
chemical composition and in sacco degradability of four varieties of cassava leaves grown in southwestern nigeria in the rumen of sheep.	the nutritive value of leaves of four varieties of cassava (ms 6, tms 30555, idileruwa and tms 30572) were studied through analysis of their chemical components and degradability of their dry matter (dm) and crude protein (cp) in the rumen of sheep. results of the chemical analyses showed that the leaves of the four varieties contained different proportions of organic matter which was significantly (p < 0.05) highest in tms 30572 with value of 935 g/kg dm, while cp (gram per kilogramme dm) was s ...	2010	20521108
cancer risk assessment of ethyl carbamate in alcoholic beverages from brazil with special consideration to the spirits cachaça and tiquira.	ethyl carbamate (ec) is a multi-site carcinogen in experimental animals and probably carcinogenic to humans (iarc group 2a). traces of ec below health-relevant ranges naturally occur in several fermented foods and beverages, while higher concentrations above 1 mg/l are regularly detected in only certain spirits derived from cyanogenic plants. in brazil this concerns the sugarcane spirit cachaça and the manioc (cassava) spirit tiquira, which both regularly exceed the national ec limit of 0.15 mg/ ...	2010	20529350
drought tolerant stem anatomy characteristics in manihot esculenta (euphorbiaceae) and a wild relative.	the stem structure of two cassava cultivars, unb 99 and unb 110, known for being adapted to humid conditions and tolerant to drought, respectively, and of a wild species, manihot glaziovii, was examined anatomically. free-hand sections of secondary stems were made, clarified with 50% sodium hypochlorite solution, stained with 1% alcian-blue safranin, and then passed through an ethanol series and butyl acetate, followed by mounting in synthetic resin. m. glaziovii stems had dense prismatic and dr ...	2010	20533197
alterations of reproduction system in a polyploidized cassava interspecific hybrid.	the objective of this research was to examine how much polyploidy may affect seed and root formation in cassava interspecific hybrids manihot esculenta crantz xm. oligantha pax. a polyploid type was induced by colchicine treatment to lateral buds followed by propagating vegetatively arising stems. cytogenetic and anatomical analyses were made on both polyploid and diploid types. the polyploid type showed extensive chromosome pairing and pollen viability. multiembryonic ovule frequency increased ...	2010	20536543
aflp-based transcript profiling for cassava genome-wide expression analysis in the onset of storage root formation.	cassava (manihot esculenta crantz) is a root crop that accumulates large quantities of starch, and it is an important source of carbohydrate. study on gene expressions during storage root development provides important information on storage root formation and starch accumulation as well as unlock new traits for improving of starch yield. cdna-amplified fragment length polymorphism (aflp) was used to compare gene expression profiles in fibrous and storage roots of cassava cultivar kasetsart 50. ...	2010	20536786
on the biomarkers and mechanisms of konzo, a distinct upper motor neuron disease associated with food (cassava) cyanogenic exposure.	konzo is a self-limiting central motor-system disease associated with food dependency on cassava and low dietary intake of sulfur amino acids (saa). under conditions of saa-deficiency, ingested cassava cyanogens yield metabolites that include thiocyanate and cyanate, a protein-carbamoylating agent. we studied the physical and biochemical modifications of rat serum and spinal cord proteins arising from intoxication of young adult rats with 50-200mg/kg linamarin, or 200mg/kg sodium cyanate (naocn) ...	2011	20538033
idiopathic chronic pancreatitis in india: phenotypic characterisation and strong genetic susceptibility due to spink1 and cftr gene mutations.	to study the genetic predisposition, phenotype and prognosis of idiopathic chronic pancreatitis (cp).	2010	20551465
efficient production of active form recombinant cassava hydroxynitrile lyase using escherichia coli in low-temperature culture.	hydroxynitrile lyase (mehnl, ec 4.1.2.39) is a useful enzyme for production of optically active cyanohydrin compounds. production of mehnl can be increased by substituting rare codons of the natural sequence of cassava (manihot esculenta) mehnl. however, most of the mehnl produced by this method was in an insoluble form in escherichia coli expression system. in order to increase the productivity of active form of mehnl, the effects of cultivation temperature were investigated. when the cultivati ...	2010	20552449