Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| a comparative study of vicilin genes in lens: negative evidence of concerted evolution. | genes for vicilin, a component of legume seed storage proteins, have been identified in the cultivated lentil (lens culinaris ssp. culinaris) and in wild species of the genus lens. five different types of vicilin sequences (designated a-e) have been identified in each lentil individual. the different types of sequences, and some possible variants of them (also present in each individual) are part of the vicilin family of genes. type d sequences have the characteristics of nonprocessed pseudogene ... | 1998 | 9501497 |
| axenic cultivation and partial characterization of leishmania braziliensis amastigote-like stages. | leishmania braziliensis strain m2903 was adapted for growth and serially maintained as amastigotes at 34 degrees c in modified um-54 medium, with growth curves exhibiting typical log and stationary phases. in late passages, amastigote growth took place in the absence of supplementary haemin and was unaffected when the initial medium ph was adjusted between 5.4 and 6.3. in contrast to promastigotes, which were elongated and exhibited very long free flagella endowed with the paraflagellar rod (pfr ... | 1998 | 9509020 |
| purification and characterization of membrane-bound semicarbazide-sensitive amine oxidase (ssao) from bovine lung. | semicarbazide-sensitive amine oxidase (ssao) has been purified from bovine lung microsomes in a form which is catalytically active and stable to storage. the enzyme, an integral membrane protein, was solubilized with triton x-100 and purification was achieved, in the presence of detergent, by chromatography with cibacron blue 3ga-agarose, hydroxylapatite, lens culinaris-agarose, resource q-fplc and gel filtration on superdex 200 hr-fplc. this is the first reported procedure for the extensive pur ... | 1998 | 9512463 |
| [clinical usefulness of lectin-reactive fraction of alpha-fetoprotein in hepatocellular carcinoma]. | alpha-fetoprotein (afp) is well known as a tumor marker of hepatocellular carcinoma (hcc). afp shows micro heterogeneity because of a structural variance in its sugar chain, and the sugar chain obtained from hcc patients has a high affinity to lectins such as lens culinaris agglutinin. the lens culinaris agglutinin a-reactive fraction of alpha-fetoprotein (afp-l3) was detected by a sensitive method using lectin-affinity electrophoresis coupled with antibody-affinity blotting. of 62 hcc patients ... | 1998 | 9528340 |
| distribution of 'free' and hla-associated human beta 2-microglobulin in some plasma membranes and biological fluids. | the distribution of free and hla-associated human beta 2-microglobulin (beta 2m) in serum, urine, spinal fluid, parotid duct saliva, seminal fluid, amniotic fluid and whey and in membranes from thrombocytes, lymphocytes, neutrophils and fat globules from milk was studied by crossed radioimmunoelectrophoresis (crie). the hydrophobic domain of hla was demonstrable in 'charge-shift crie' and by binding to phenyl-sepharose in 'hydrophobic-interaction crie'. in 'lectin-affinity crie' with concanavali ... | 1980 | 9537063 |
| lectin histochemistry in the aged dog brain. | formalin-fixed and paraffin-embedded canine brains were examined histochemically using 15 selected lectins. concanavalin a (con a), lens culinaris agglutinin, lycopersicon esculentum agglutinin (lel) and limulus polyphemus agglutinin (lpa) labeled neurons in an age-dependent manner. these and some other lectins [dolichos biflorus agglutinin (dba), vicia villosa agglutinin (vva), ricinus communis agglutinin 120 (rca-i), bandeiraea simplicifolia agglutinin (bsl-i), and phaseolus vulagaris agglutin ... | 1998 | 9542591 |
| developmental alterations of alpha-fetoprotein sugar chain in amniotic fluids analyzed by lectin affinity electrophoresis. | affinity electrophoresis of human alpha-fetoprotein (afp) in amniotic fluid from pregnant women between 6 to 42 weeks of gestation and in the serum of a yolk sac tumor was performed with concanavalin a (con a), lentil lectin (lca), erythroagglutinating phytohemagglutinin (e-pha) and allomyrina dichotoma lectin (allo a). separated afp bands were detected by sensitive antibody-affinity blotting. in the first trimester, amniotic fluid afp showed elevated percentages of con a-nonreacting afp (afp-c1 ... | 1998 | 9548991 |
| decreased symbiotic effectiveness of rhizobium leguminosarum strains carrying plasmid rp4. | the presence of derivatives of the broad host range plasmid rp4 in strains of rhizobium leguminosarum biovar viciae severely inhibited nitrogen fixation by these strains in nodules on cultivars of pea (pisum sativum). the strains formed small white nodules. yield and total nitrogen values were comparable with those obtained for plants inoculated with a non-nodulating mutant. strains carrying the same derivatives gave rise to nitrogen fixing nodules when inoculated on cultivars of lentils (lens c ... | 1998 | 9570119 |
| cortical granules reaction after intracytoplasmic sperm injection. | the cortical reaction (cr) in mammalian oocytes is induced following sperm-egg membrane fusion. during intracytoplasmic sperm injection (icsi) the physiological cascade of gamete interaction events is bypassed. the aim of this study was to explore cr occurrence after the icsi procedure and its correlation with the meiotic status of the oocytes. unfertilized and abnormally fertilized (one- or three-pronucleate) human oocytes were investigated. the chromosomal status was analysed by hoechst staini ... | 1998 | 9570275 |
| developmental changes in the glycosylation of glycoprotein hormone free alpha subunit during pregnancy. | glycoprotein hormone alpha subunit, in its free form (free alpha), is a major placental product. its glycosylation was found to change dramatically during the advancement of pregnancy. in this study, we have analyzed these glycosylation changes in five normal pregnancies. binding to lens culinaris lectin increased dramatically in all subjects between weeks 14 and 17 from the last menstrual period, indicating more core fucosylation as well as possible changes in branching of glycans. studies usin ... | 1998 | 9575149 |
| intermediates in the catalytic cycle of lentil (lens esculenta) seedling copper-containing amine oxidase. | spectrophotometry and rapid-scanning stopped-flow spectroscopy have been used to investigate the visible absorbance changes that occur in the course of the reduction of lentil (lens esculenta) seedling amine oxidase by substrate. the catalytic cycle of the enzyme employs several intermediates but, owing to kinetic limitations, some of them were not identified in previous studies. in this study we have examined several substrates, either rapidly reacting (e.g. putrescine) or slowly reacting (e.g. ... | 1998 | 9601072 |
| automatic and simultaneous analysis of lens culinaris agglutinin-reactive alpha-fetoprotein ratio and total alpha-fetoprotein concentration. | an automated analytical method for analyzing alpha-fetoprotein (afp) carbohydrate chain microheterogeneity based on competitive reaction between lectin and anti-afp monoclonal antibody in liquid phase is described. the antibody used binds to all species of afp molecule without lens culinaris agglutinin (lca); however, its binding reaction to lca-reactive afp was inhibited by lca. sulfated tyrosine octamer was conjugated to the antibody, and sulfated tyrosine pentamer and peroxidase were conjugat ... | 1998 | 9608849 |
| carbohydrate induced modulation of cell membrane vii. binding of exogenous lectin increases osmofragility of erythrocytes. | due to their multivalent binding character, lectins when added exogenously will cross-link membrane surface receptors leading to lateral molecular reorganizations in the plane of the bilayer. this study reports for the first time that agglutination of rabbit erythrocytes by lentil lectin and concanavalin a increases their osmofragility. increase in osmofragility was detected by measuring the hemolysis of erythrocytes in hypotonic as well as in isotonic solutions. it was also found that agglutina ... | 1998 | 9613592 |
| mannose-specific lectins modulate ligand binding to ampa-type glutamate receptors. | binding of [3h]ampa was increased above control levels in rat brain membranes that had been incubated with concanavalin a (con a) or a lectin from lens culinaris (lc), both of which bind mannose residues. this did not occur with any of six lectins with other specificities. the magnitude of the increased binding varied from 15% in cortex to 70% in hippocampus and decreased significantly between 3 weeks and 6 months of age. succinylated con a was without effect and neither con a nor lc increased b ... | 1998 | 9622605 |
| kinetics of cytokine and nfat gene expression in human interleukin-2-dependent t lymphoblasts stimulated via t-cell receptor. | t cells respond to mitogenic or antigenic stimulation by proliferation and by turning on cytokine gene expression. here we have analysed the kinetics and nature of cytokine production in human peripheral blood-derived t lymphoblasts stimulated with anti-cd3 antibodies or lens culinaris lectin (lcl). t cells were purified from peripheral blood mononuclear cells (pbmc) and primarily activated with anti-cd3 antibodies and cultured in the presence of interleukin-2 (il-2). anti-cd3-restimulated t cel ... | 1998 | 9640245 |
| half-embryo cocultivation technique for estimating the susceptibility of pea (pisum sativum l.) and lentil (lens culinaris medik.) cultivars to agrobacterium tumefaciens. | longitudinally sliced embryonic axes from pea and lentil mature seeds cocultivated with a. tumefaciens carrying a gus reporter gene in its t-dna provided a convenient means to evaluate the efficiency of gene transfer to tissues in different cultivars and cocultivation conditions. use of this technique demonstrated wide variation in susceptibility to agrobacterium among several pea and lentil commercial genotypes. | 1998 | 9658395 |
| relationship between lens culinaris agglutinin reactive alpha-fetoprotein and biological features of hepatocellular carcinoma. | lens culinaris agglutinin-reactive fraction of alpha-fetoprotein (afp-l3), which is a fucosylated variation of afp, is not only sensitive and specific for localization of hepatocellular carcinoma (hcc) but also a prognostic factor for patients with hcc. the relationship between status of afp-l3% in serum and pathological findings was studied using 48 resected hcc specimens. afp-l3 fraction was measured by lectin-affinity blotting using an afp differentiation kit l (wako, osaka, japan), and was e ... | 1998 | 9658760 |
| lectin-dependent modulation of interaction between human alpha-fetoprotein and its monoclonal antibodies. epitope mapping. | the effect of lentil lectin (lca) on the binding of mouse monoclonal antibody (moab) against human alpha-fetoprotein (afp) to lca-nonreactive afp-l1 and lca-reactive afp-l3 was studied on a panel of 30 moabs provided by the td-2 workshop of isobm for epitope mapping. lca inhibited the binding of moabs 93 and 98 to afp-l3 but not to afp-l1, indicating that there was a competition between the moabs and lca for the afp sugar chain. with moabs 100, 109, 118, and 120, lca rather increased the binding ... | 1998 | 9679744 |
| developmental expression and biochemical analysis of the arabidopsis atao1 gene encoding an h2o2-generating diamine oxidase. | a copper amine oxidase encoding gene, atao1, has been isolated and characterized from arabidopsis thaliana. sequence analysis reveals that atao1 encodes a 668 amino acid polypeptide (atao1) with 48% identity to copper amine oxidases from pea and lentil. the promoter region of atao1 was transcriptionally fused with the reporter genes encoding beta-glucuronidase and modified green fluorescent protein. analysis of transgenic arabidopsis together with in situ hybridization of wild-type plants reveal ... | 1998 | 9681017 |
| natural polyamines inhibit soybean (glycine max) lipoxygenase-1, but not the lipoxygenase-2 isozyme. | natural polyamines are shown to inhibit dioxygenase activity of soybean lipoxygenase-1, but they were ineffective toward the lipoxygenase-2 isozyme. the inhibitory power was dependent on the number of basic groups in the molecule, in the order spermine > spermidine > cadaverine >/= putrescine. both spermidine and spermine acted as uncompetitive inhibitors of lipoxygenase-1 with respect to linoleic acid, the inhibition constants being 2.70 and 0.80 mm, respectively. the inhibitory power apparentl ... | 1998 | 9681988 |
| tumor vascularity and lens culinaris agglutinin reactive alpha-fetoprotein are predictors of long-term prognosis in patients with hepatocellular carcinoma after percutaneous ethanol injection therapy. | percutaneous ethanol injection therapy (peit) is now widely used for small hepatocellular carcinoma (hcc). however, only limited information is available regarding predictors of long-term prognosis of patients with small hcc after peit. the relationship of pretreatment clinicopathologic and biologic factors (age, sex, virus marker, child-pugh classification, tumor size, number of tumor, histologic grade, tumor staining, alpha-fetoprotein level, afp-l3%, and ki67 labelling index) to long-term pro ... | 1998 | 9715046 |
| gene expression of alpha1-6 fucosyltransferase in human hepatoma tissues: a possible implication for increased fucosylation of alpha-fetoprotein. | the 1-6 fucosylated -fetoprotein (afp) present in serum of patients with hepatocellular carcinoma (hcc) has been employed for the differential clinical diagnosis of hcc from chronic liver diseases. the molecular mechanism by which this alteration occurs, however, remains largely unknown. to address this issue, we purified gdp-l-fuc:n-acetyl-beta-d-glucosaminide 1-6 fucosyltransferase (1-6 fuct), an enzyme involved in the 1-6 fucosylation of n-glycans from porcine brain, as well as from a human g ... | 1998 | 9755230 |
| mechanisms leading to cortical reaction in the mammalian egg. | activation of the mammalian egg results in cortical reaction (cr), which is correlated with an increase in intracellular ca2+ concentration and pkc activation. the cr is a gradual rather then an "all or none" response, and can be regulated by different concentrations of parthenogenetic activators. to evaluate the biological significance of parthenogenetic induced cr, rat eggs were fertilized or activated by different concentrations of ionomycin and tpa. cortical granules (cg) were monitored by e ... | 1998 | 9771650 |
| induction of a germination specific, low molecular weight, acid phosphatase isozyme with specific phosphotyrosine phosphatase activity in lentil (lens esculenta) seeds. | a germination specific isozyme of acid phosphatase (ec 3.1.3.2) hydrolysing o-phospho-l-tyrosine, ph optima 5.5 is induced in lentil seeds. when seeds at 0 h, 24 h and 36 h of germination are electrophorezed, native page on specific enzyme staining shows several constitutive isozymes of acid phosphatases. at 48 h, an isozyme is induced which gradually decreases and then disappears at 108 h of germination. the short lived, induced isozyme is present in the embryo and seed-coat but not in the plum ... | 1998 | 9784397 |
| inhibitors of plant copper amine oxidases. | in this review, inhibitors of plant copper amine oxidases from lens esculenta seedlings, pisum sativum seedlings, and euphorbia characias latex are described. reversible competitive inhibitors and non-competitive inhibitors, irreversible active-site directed inhibitors and mechanism-based inactivators are reviewed in regard to their mechanisms of action. | 1998 | 9793836 |
| lectin-mediated bioadhesion: binding characteristics of plant lectins on the enterocyte-like cell lines caco-2, ht-29 and hct-8. | in order to take advantage of the biorecognition between lectins and specific carbohydrates for targeted drug delivery, fluorescein-labelled lectins of different carbohydrate specificities were screened for binding to human colorectal carcinoma cell lines by flow cytometry and confocal microscopy. the lectin-binding rate increased as follows: dolichos biflorus agglutinin, dba<peanut agglutinin, pna<lens culinaris agglutinin, lca<solanum tuberosum lectin, stl<ulex europaeus isoagglutinin i, uea-i ... | 1998 | 9795030 |
| dynamics of the lens culinaris agglutinin-lactotransferrin and serotransferrin complexes, followed by fluorescence intensity quenching of fluorescein (fitc) with iodide and temperature. | dynamics of the fluorescent lens culinaris agglutinin-fluorescein complex (lca-fitc) are studied in absence and in presence of two glycoproteins, lactotransferrin (ltf) and serotransferrin (stf). glycans of the serotransferrin are not fucosylated, while those of the lactotransferrin have an alpha-1,6-fucose bound to the n-acetylglucosamine residue linked to the peptide chain, and an alpha-1,3-fucose bound to the n-acetyllactosamine residues. interaction between the lectin and the two glycoprotei ... | 1998 | 9795256 |
| antral follicle development influences plasma membrane organization but not cortical granule distribution in mouse oocytes. | in the present study, we evaluated the contributions of antral follicle development and antral granulosa cell-released factor(s) to the acquisition of a mature mouse oocyte plasma membrane organization and cortical granule distribution. this has been performed by comparing in-vitro matured oocytes derived from early antral follicles (here referred to as denuded oocytes) or from pre-ovulatory follicles, and cultured either as cumulus-intact or cumulus-free oocytes, with in-vivo ovulated eggs. by ... | 1998 | 9804243 |
| application of an immunoassay to determine risk factors associated with porcine cysticercosis in rural areas of yucatan, mexico. | a western blot assay for identification of 26 kda and/or 8 kda antigens in a crude saline extract (cse) of taenia solium metacestodes, previously developed for human cysticercosis, was evaluated for use with pigs. the test population used for assay standardisation consisted of 45 cysticercotic pigs (5 pigs positive by necropsy and 40 selected by tongue palpation and ll-gp immunoblot but not confirmed by necropsy), 48 pigs with heterologous infections and 40 negative controls from endemic and non ... | 1998 | 9806496 |
| competitive inhibition of lens culinaris l. copper amine oxidase by amiloride, p-aminobenzamidine, clonidine, 4',6-diamidino-2-phenylindole and gabexate mesylate: a comparative study. | the competitive inhibition of lens culinaris l. copper amine oxidase by amiloride (ki = 4.1 x 10(-4) m), p-aminobenzamidine (ki = 6.0 x 10(-4) m), clonidine (ki = 5.0 x 10(-4) m), 4',6-diamidino-2-phenylindole (dapi; ki = 1.9 x 10(-5) m) and gabexate mesylate (ki = 2.5 x 10(-4) m) has been investigated, at ph 7.0 and 25 degrees c. the affinity of p-aminobenzamidine, clonidine and dapi for plant and mammalian copper amine oxidase is closely similar. however, values of ki for amiloride and gabexat ... | 1998 | 9825309 |
| the fucosylation index of alpha-fetoprotein as a possible prognostic indicator for patients with hepatocellular carcinoma. | the aim of this study was to elucidate the usefulness of measuring the fucosylation index (fi) of alpha-fetoprotein (afp) before the initiation of therapy as a new prognostic indicator for patients with hepatocellular carcinoma (hcc). | 1998 | 9827711 |
| [soluble, insoluble and total dietary fiber in raw and cooked legumes]. | soluble (sdf), insoluble (idf) and total dietary fiber (tdf) were analysed in 26 samples of the following legumes: peas (pisum sativum l) coated and uncoated; beans (phaseolus vulgaris l), beans (vigna sinesis l), chick peas (cicer arietinum l), lentils (lens culinaris l) and pigeon peas (cajanus indicus l) raw and cooked, purchased at wholesale level. the aoac enzimatic-gravimetric method (1990) was used. the cooked grains were drained and dried before analysis. values for tdf in the raw legume ... | 1998 | 9830497 |
| separation of disaccharides by affinity capillary electrophoresis in lectin-containing electrophoretic solutions. | separation of the 1-phenyl-3-methyl-5-pyrazolone (pmp) derivatives of simple disaccharides (maltose, cellobiose, gentiobiose, lactose, and melibiose) by affinity capillary electrophoresis was investigated using lectin-containing neutral phosphate buffers, filled in a linear polyacrylamide-coated capillary. when lens culinaris agglutinin (lca) was added, the derivatives of glucobioses were retarded with varying magnitudes depending on the amount of lca and were well separated from each other and ... | 1998 | 9848673 |
| allergy to lentils in mediterranean pediatric patients. | peanuts and soybeans are the major legumes involved in human food allergy; however, scarce data exist on adverse reactions to other temperate legumes, such as lentils. | 1999 | 9893199 |
| lentil lectin enriched microsomes from the plasma membrane of the human b-lymphocyte cell line h2lcl carry a heavy load of type-1 porin. | using an established biochemical approach, five subcellular fractions of human b lymphocytes were prepared by differential centrifugation. crude membranes were passed over a lentil lectin column to enrich carbohydrate-coated cell surface microsomes. the lectin-bound fraction contained a high amount of plasma membrane-derived microsomes as indicated by cell surface markers. all subcellular fractions in western blots proved to contain distinct but variable amounts of porin. there was a strong incr ... | 1998 | 9894809 |
| evaluation of curability and prediction of prognosis after surgical treatment for hepatocellular carcinoma by lens culinaris agglutinin-reactive alpha-fetoprotein. | the clinical significance of serum lens culinaris agglutinin-reactive alpha-fetoprotein (afp-l3), which can distinguish between hepatocellular carcinoma and hepatitis by detecting a sugar chain micro heterogeneity, was evaluated for its possible ability to recognize previously undetectable residual tumors, and for increasing the accuracy of prognosis after surgical treatment for hepatocellular carcinoma. serum lens culinaris agglutinin-reactive alpha-fetoprotein was measured pre- and post-operat ... | 1999 | 9917501 |
| clinical utility of lens culinaris agglutinin-reactive alpha-fetoprotein in small hepatocellular carcinoma: special reference to imaging diagnosis. | blood concentration levels of alpha-fetoprotein like the lens culinaris agglutinin-reactive fraction (afp-l3) are a useful marker for predicting the long-term prognosis of hepatocellular carcinoma. this study investigated the relationship between serum afp-l3 and various imaging modalities. | 1999 | 9927159 |
| detection of disease-specific augmentation of abnormal immunoglobulin g in sera of patients with rheumatoid arthritis. | galactose-free immunoglobulin g (igg), which is known to be higher in the sera of patients with rheumatoid arthritis, was prepared from igg of healthy volunteers using enzymes. its reactivity to lectins was analyzed. the galactose-free igg showed no reactivity to ricinus communis agglutinin 120 but displayed greater reactivity to concanavalin a and lens culinaris lectin than did intact human igg. then, igg in serum samples was bound to protein a immobilized on a nitrocellulose membrane, and its ... | 1998 | 10052597 |
| quantification of brca1 protein in sporadic breast carcinoma with or without loss of heterozygosity of the brca1 gene. | in sporadic breast cancer, no mutations of the brca1 gene have been reported so far, whereas brca1 mrna is markedly decreased in invasive breast cancer. to elucidate the contribution of the brca1 gene in sporadic breast cancer, we quantified the brca1 protein, using [125i] labeling of whole-cell proteins, lentil-lectin affinity chromatography, immunoprecipitation by anti-brca1 antibodies (c-20, d-20, i-20 and k-18), purification of the immune complex by protein a affinity chromatography and chro ... | 1999 | 10074913 |
| dietary lectins can induce in vitro release of il-4 and il-13 from human basophils. | dietary lectins, present in beans and other edible plant products, pose a potential threat to consumers due to their capacity to induce histamine release from basophils. in this study, we analyzed the capacity of 16 common, in particular dietary, lectins to induce human basophils to secrete il-4 and il-13, the key promoters of th2 responses and ige synthesis. several of the lectins, especially concanavalin a, lentil lectin, phytohemagglutinin, pisum sativum agglutinin and sambucus nigra agglutin ... | 1999 | 10092096 |
| comparison of kinetic properties of amine oxidases from sainfoin and lentil and immunochemical characterization of copper/quinoprotein amine oxidases. | kinetic properties of novel amine oxidase isolated from sainfoin (onobrychis viciifolia) were compared to those of typical plant amine oxidase (ec 1.4.3.6) from lentil (lens culinaris). the amine oxidase from sainfoin was active toward substrates, such as 1,5-diaminopentane (cadaverine) with k(m) of 0.09 mm and 1,4-diaminobutane (putrescine) with k(m) of 0.24 mm. the maximum rate of oxidation for cadaverine at saturating concentration was 2.7 fold higher than that of putrescine. the amine oxidas ... | 1999 | 10092944 |
| zinc supplementation in malnourished children with persistent diarrhea in pakistan. | to evaluate the potential benefit of dietary supplementation of a rice-lentil (khitchri) and yogurt diet with 3 mg/kg/d of elemental zinc (as zinc sulfate) in hospitalized malnourished children (age 6-36 months) with persistent diarrhea for 14 days. | 1999 | 10103334 |
| expression of the functional extracellular domain of human thyrotropin receptor using a vaccinia virus system: its purification and analysis of autoantibody binding. | we produced substantial amount of the extracellular domain of the human tsh receptor (tshre) that has a tag of six histidines at c-terminus as a soluble form in the human cell line hela using a vaccinia virus system. by sequential nickel-chelating and lentil lectin column chromatography, tshre was purified to about 70% purity, with the recovery of around 0.1-0.2 mg tshre/l culture (5 x 10(8) cells/liter culture). the purified tshre interacted with tsh as well as graves' autoantibodies to tshr. h ... | 1999 | 10199784 |
| phenotypic characteristics of coagulase-negative staphylococci: typing and antibiotic susceptibility. | the present thesis deals with various aspects of handling coagulase-negative staphylococci (cons) in the local clinical microbiology laboratory. cons are normal inhabitants of the skin and mucus membranes and are increasingly being recognised as opportunistic pathogens causing infection in the immunocompromised host, in particular patients with indwelling plastic devices. in particular the finding of cons in specimens which should normally be sterile, such as blood cultures, is of interest. the ... | 1999 | 10230367 |
| alpha-fetoprotein and beta-human chorionic gonadotropin: their clinical significance as tumour markers. | tumour markers can aid in areas such as diagnosis, surveillance of recurrence, staging and prognosis. this article focuses on 2 tumour markers, alpha-fetoprotein (afp) and beta-human chorionic gonadotropin (hcg). these tumour markers have been examined for their utility as prognostic indicators in 2 different manners. first, the marker level at diagnosis has been studied to determine if it is prognostic of outcome presumably because of its relation to tumour bulk or to the biological nature of t ... | 1999 | 10235686 |
| differentiation of helicobacter pylori isolates based on lectin binding of cell extracts in an agglutination assay. | plant and animal lectins with various carbohydrate specificities were used to type 35 irish clinical isolates of helicobacter pylori and the type strain nctc 11637 in a microtiter plate assay. initially, a panel of eight lectins with the indicated primary specificities were used: anguilla anguilla (aaa), lotus tetragonolobus (lotus a), and ulex europaeus i (uea i), specific for alpha-l-fucose; solanum tuberosum (sta) and triticum vulgaris (wga), specific for beta-n-acetylglucosamine; glycine max ... | 1999 | 10325361 |
| purification and characterization of stachyose synthase from lentil (lens culinaris) seeds: galactopinitol and stachyose synthesis. | stachyose synthase (sts) (ec 2.4.1.67) was purified 313-fold from mature seeds of lentil. the final preparation had a specific activity of 9.09 nkat stachyose formed per milligram of protein. the enzyme was a monomeric protein with a molecular mass of 88.6 kda (sds-page) and an isoelectric point of 4.8 (chromatofocusing). western analysis revealed cross-reactivity of polyclonal antibodies raised against sts from adzuki bean with the lentil enzyme. the purified enzyme catalyzed a range of differe ... | 1999 | 10334866 |
| interaction between calcofluor white and carbohydrates of alpha 1-acid glycoprotein. | interactions between the fluorescent probe, calcofluor white, and human serum albumin (hsa) and alpha 1-acid glycoprotein (orosomucoid) are compared. the two proteins have comparable isoelectric points, but alpha 1-acid glycoprotein is highly glycosylated (40% of glycans by weight), while the serum albumin is not. binding of calcofluor to the proteins induces an increase in both the fluorescence anisotropy and the fluorescence intensity of the fluorophore. also, we found that the calcofluor exhi ... | 1998 | 10335587 |
| surface ph at the basolateral membrane of the caecal mucosa of guinea pig. | since the major mechanisms responsible for regulation of intracellular ph of enterocytes are located in the basolateral membrane, respective effects may be expected on ph in the compartment near the basolateral membrane. a method was established to estimate the ph at the basolateral membrane (phb) of isolated caecal epithelia of guinea pig using ph-sensitive fluorescein attached to lectin (lens culinaris). in the presence of bicarbonate and a perfusion solution-ph of 7.4, phb was 7.70 +/- 0.15. ... | 1999 | 10341033 |
| highly enhanced fucosylation of serum glycoproteins in patients with hepatocellular carcinoma. | we recently reported that the measurement of lens culinaris agglutinin-reactive species of alpha-fetoprotein (afp), alpha-1-antitrypsin (aat) and transferrin (tf) is useful for the diagnosis of hepatocellular carcinoma (hcc) and that the molecular basis for this reactivity is fucosylation at the innermost n-acetylglucosamine residue of a biantennary sugar chain. however, the precise relationship of the fucosylation of afp, aat and tf in patients with hcc and liver cirrhosis is not fully understo ... | 1999 | 10355508 |
| evidence for the presence of major peripheral myelin glycoprotein p0 in mammalian spinal cord and a change of its glycosylation state during aging. | glycoproteins, which react with lens culinaris agglutinin, in the membrane preparation of various portions of brains and spinal cords, obtained from 9-week-old rats and 29-month-old rats, were comparatively analyzed by sds-polyacrylamide gel electrophoresis. in contrast to the samples from brain, which showed similar staining patterns in the two different age groups, the glycoprotein patterns of spinal cords showed marked differences by the age of donors. the most prominent evidence is that a gl ... | 1999 | 10362834 |
| interactions between carbonic anhydrase and some decarboxylating enzymes as studied by a new bioelectrochemical approach. | this work presents the results of a study, carried out by recently developed amperometric bioelectrodes, on the interactions between carbonic anhydrase (ca) and the decarboxylating enzymes arginine decarboxylase (adc), l-lysine decarboxylase (ldc), and l-ornithine decarboxylase (odc). these are all pyridoxal-phosphate dependent enzymes and catalyze the decarboxylation reaction of the respective amino acids, to give carbon dioxide and the corresponding diamine (agmatine, cadaverine, and putrescin ... | 1999 | 10379569 |
| identification of two nonglycosylated polypeptides of taenia solium recognized by immunoglobulins from patients with neurocysticercosis. | an immunoprecipitation technique using biotin-labeled proteins of taenia solium was developed to identify antigens recognized by immunoglobulins from patients with neurocysticercosis. six major polypeptides of 100, 70, 50, 42, 35, and 24 kda were recognized by cerebrospinal fluid from most serologically positive patients. all polypeptides except the 70- and 35-kda antigens were retained on a lentil-lectin chromatography column and were recognized by lentil lectin in an overlay assay. the 70- and ... | 1999 | 10382599 |
| cui-semiquinone radical species in plant copper-amine oxidases. | the intermediate cui-semiquinone radical species in the catalytic mechanism of copper-amine oxidase from lens esculenta and pisum sativum seedlings has been studied by optical, raman resonance and esr spectroscopies and by stopped-flow and temperature-jump measurements. treatment of highly purified enzyme preparations with good, poor or suicide substrates, under anaerobic and aerobic conditions, at different ph values and temperatures, makes it possible to generate, detect and characterize this ... | 1999 | 10403363 |
| developmental alterations in the alpha-fetoprotein sugar chain in maternal serum analyzed by lectin affinity electrophoresis. | our purpose was to investigate developmental alterations of human alpha-fetoprotein (afp) oligosaccharides in maternal serum by lectin affinity electrophoresis and to compare the afp glycoforms in maternal serum with those in umbilical cord serum and amniotic fluid. afp glycoforms were separated by affinity electrophoresis with concanavalin a (con a), lentil lectin (lca), erythroagglutinating phytohemagglutinin (e-pha) and allomyrina dichotoma lectin (allo a) and detected by sensitive antibody a ... | 1999 | 10410786 |
| purification of xyloglucan endotransglycosylase based on affinity sorption of the active glycosyl-enzyme intermediate complex to cellulose. | xyloglucan endotransglycosylase (xet) catalyzes the cleavage of xyloglucan (xg) molecules by a transglycosylation mechanism involving two steps: (a) endocleavage of the beta-(1,4)-linked polyglucosyl backbone of the xyloglucan molecule with formation of a glycosyl-enzyme intermediate; (b) transfer of the glycosyl residue from the intermediate to the c-4 position of the nonreducing end glucosyl unit of another molecule of xg or an xg-derived oligosaccharide with liberation of the enzyme (z. sulov ... | 1999 | 10419819 |
| specific staining of nonpyramidal cell populations of the cerebral cortex by lectin cytochemistry on semithin sections. | the pattern of lectin labeling in the cerebral cortex of the cat was studied using semithin sections. the labeling produced by some lectins (concanavalin a, lens culinaris, phaseolus vulgaris-l, phaseolus vulgaris-e, pisum sativum, wheat germ agglutinin, and succynilated-wheat germ) appeared inside every neuron as small cytoplasmic granules, probably corresponding to cisterns of endoplasmic reticulum and/or the golgi complex. lectins with affinity for alpha-mannosyl residues (pisum sativum, lens ... | 1999 | 10424845 |
| a putative role for carbohydrates in sea urchin gastrulation. | many studies have examined the effects of lectins on embryonic development. recently, it has been shown that lectins actually enter the blastocoel of sea urchin embryos without microinjection and bind to specific cell types. the present study was performed to examine the effects of lectins on sea urchin gastrulation. strongylocentrotus purpuratus sea urchin embryos were incubated with several lectins at concentrations from 0.01 microgram/ml to 100 micrograms/ml at 15-28 h in the presence or abse ... | 1999 | 10443292 |
| a histidine thiol 100 kda, tetrameric acid phosphatase from lentil, lens esculenta, seeds with the characteristics of protein tyrosine phosphatases. | a non-specific acid phosphatase (apase) hydrolysing l-tyrosine-o-phosphate and 3'-amp was purified to electrophoretic homogeneity from mature lentil seeds with apparent native molecular mass of 100 kda and subunit molecular mass of 24 kda. these activities appear to reside on the same protein which shows a single band in native and sds-page. the ph optimum is 5.5, while the k(m) (mm) and v(max) (micromoles/min/mg protein) for p-nitrophenyl phosphate (pnpp) are 0.7 and 9.2 and for l-tyrosine-o-ph ... | 1999 | 10446377 |
| increased butyrylcholinesterase levels in microsomal membranes of dystrophic lama2dy mouse muscle. | the proportions and the glycosylation of butyrylcholinesterase (buche) forms in vesicles rich in sarcoplasmic reticulum from normal (nmv) and dystrophic (dmv) muscle were analyzed, using merosin-deficient dystrophic mice. buche activity in dmv was two- to threefold that in nmv. globular amphiphilic g1a, g2a, and g4a and hydrophilic g4h buche forms were identified in nmv and dmv. the amount of g2a forms increased sevenfold in dmv, and the other forms increased about twofold. the higher buche leve ... | 1999 | 10461905 |
| new detection method after cellulose acetate membrane isoelectric focusing: activity staining for lactate dehydrogenase, detection for sugar chains using lectin and simple western blotting. | to extend the clinical applications for cellulose acetate (ca) membrane isoelectric focusing, we designed two detection methods and two blotting methods that improve the characteristics of the ca membrane; that is, detection of the lactate dehydrogenase (ld) isozyme on the ca membrane, detection of the sugar chain in glycoprotein on the ca membrane, and simple and rapid methods for western blotting. in the detection of the ld isozyme, when 50% saturated ammonium sulfate solution was used as a fi ... | 1999 | 10494133 |
| interaction between calcofluor white and carbohydrates of alpha 1-acid glycoprotein. | interactions between the fluorescent probe, calcofluor white, and human serum albumin (hsa) and alpha 1-acid glycoprotein (orosomucoid) are compared. the two proteins have comparable isoelectric points, but alpha 1-acid glycoprotein is highly glycosylated (40% of glycans by weight), while the serum albumin is not. binding of calcofluor to the proteins induces an increase in both the fluorescence anisotropy and the fluorescence intensity of the fluorophore. also, we found that the calcofluor exhi ... | 1999 | 10515058 |
| usefulness of measurement of lens culinaris agglutinin-reactive fraction of alpha-fetoprotein as a marker of prognosis and recurrence of small hepatocellular carcinoma. | lens culinaris agglutinin-reactive fraction of alpha-fetoprotein (afp-l3%) is a recently described marker of hepatocellular carcinoma (hcc), and its usefulness has been demonstrated in many studies. we evaluated the usefulness of serial measurement of afp-l3% as a marker of prognosis and recurrence after treatment of small hcc. | 1999 | 10520864 |
| usefulness of lens culinaris agglutinin a-reactive fraction of alpha-fetoprotein (afp-l3) as a marker of distant metastasis from hepatocellular carcinoma. | the objective of this study was to clarify the relationship between the serum level of the lens culinaris agglutinin a-reactive fraction of alpha-fetoprotein (afp-l3) and the clinical features including sex, age, child's classification, virus markers, tumour size, tumour stage, distant metastasis, histopathologic findings, portal thrombus and outcome of hepatocellular carcinoma (hcc). we measured the afp-l3 levels in 170 hcc cases at the time of diagnosis using lectin-affinity electrophoresis fo ... | 2015 | 10523686 |
| determination of epsilon-n-pyrrolylnorleucine in fresh food products. | epsilon-n-pyrrolylnorleucine was determined in different fresh food products to study its presence as a normal component of food proteins. twenty-two different products were screened: cod, cuttlefish, salmon, sardine, trout, beef, chicken, pork, broad bean, broccoli, chickpea, garlic, green pea, lentil, mushroom, soybean, spinach, sunflower, almond, hazelnut, peanut, and walnut. foods were homogenized, their proteins were precipitated with trichloroacetic acid and hydrolyzed with 2 n naoh for 20 ... | 1999 | 10552475 |
| specific ige determinations to crude and boiled lentil (lens culinaris) extracts in lentil-sensitive children and controls. | the aims of this study were to evaluate the allergenicity of boiled and crude lentil extracts and to compare specific ige binding in tolerant and nontolerant lentil-allergic children. | 1999 | 10604559 |
| nutritional parameters and yield evaluation of newly developed genotypes of lentil (lens culinaris medik.). | among the commonly consumed food legumes, lentils occupy an important place in human nutrition in the indian subcontinent. twenty-one lentil genotypes were evaluated for such nutrition related parameters as moisture, protein, crude fiber, fat, ash (total mineral matter), carbohydrates, total energy and metabolizable energy. these genotypes were also analyzed for 100-seed weight and seed yield/plant. protein content ranged between 22.1 and 27.4% with significant differences among genotypes. consi ... | 1999 | 10646632 |
| clinical utility of simultaneous measurement of serum high-sensitivity des-gamma-carboxy prothrombin and lens culinaris agglutinin a-reactive alpha-fetoprotein in patients with small hepatocellular carcinoma. | to evaluate the diagnostic efficacy of simultaneous measurements of high-sensitivity des-gamma-carboxy prothrombin (h-dcp) and lens culinaris agglutinin a-reactive alpha-fetoprotein (afp-l3) in small hepatocellular carcinoma (hcc). | 1999 | 10654799 |
| identification and expression of isoflavone synthase, the key enzyme for biosynthesis of isoflavones in legumes. | isoflavones have drawn much attention because of their benefits to human health. these compounds, which are produced almost exclusively in legumes, have natural roles in plant defense and root nodulation. isoflavone synthase catalyzes the first committed step of isoflavone biosynthesis, a branch of the phenylpropanoid pathway. to identify the gene encoding this enzyme, we used a yeast expression assay to screen soybean ests encoding cytochrome p450 proteins. we identified two soybean genes encod ... | 2000 | 10657130 |
| lectin-binding patterns in normal, hyperplastic and neoplastic endometrium: the prognostic value of concanavalin a. | lectins are proteins and glycoproteins of non-immune origin which bind specifically to carbohydrate residues, agglutinate cells and/or precipitate complex carbohydrates. lectin-binding patterns in normal, hyperplastic and neoplastic endometria were studied using four biotinylated lectins (con a, lca, e-pha, l-pha) and the avidin-biotin-peroxidase technique. canavalia ensiformis agglutinin (cona) and lens culinaris agglutinin (lca) reacted strongly with the luminal borders and the cytoplasm of ep ... | 2000 | 10664162 |
| a simultaneous monitoring of lens culinaris agglutinin a-reactive alpha-fetoprotein and des-gamma-carboxy prothrombin as an early diagnosis of hepatocellular carcinoma in the follow-up of cirrhotic patients. | to elucidate the risk factors for developing hepatocellular carcinoma (hcc) during the follow-up of patients with liver cirrhosis (lc), outpatients with lc were examined periodically by means of serum biochemical assessments, ultrasonography, and computed tomography. risk factors for hcc were statistically analyzed. we also examined an efficacy of lens culinaris agglutinin a-reactive profiles of alpha-fetoprotein (afp-l3%) and des-gamma-carboxy prothrombin (dcp) value using a highly sensitive dc ... | 2015 | 10671666 |
| purification of respiratory syncytial virus f and g proteins. | respiratory syncytial virus (rsv) is the most important cause of severe lower respiratory tract infections of infants in industrial nations. in addition, the participation of rsv in the genesis of asthma is under discussion. the rsv glycoproteins f and g have key positions in the viral pathogenesis. at present no satisfactory protein purification protocols are available for these proteins. the methods published for the g protein using preparative sds-page or immunoaffinity chromatography yield o ... | 2000 | 10681046 |
| effects of lectins with different carbohydrate-binding specificities on hepatoma, choriocarcinoma, melanoma and osteosarcoma cell lines. | the effects of lectins with different carbohydrate-binding specificities on human hepatoma (h3b), human choriocarcinoma (jar), mouse melanoma (b16) and rat osteosarcoma (ros) cell lines were investigated. cell viability was estimated by uptake of crystal violet. wheat germ lectin was the lectin with the most deleterious effect on the viability of h3b, jar and ros cell lines. the cytotoxicity of lectins with similar sugar-binding specificity to wheat germ lectin, including maackia amurensis lecti ... | 2000 | 10716633 |
| free amino acids present in commercially available seedlings sold for human consumption. a potential hazard for consumers. | the importance of fresh seedlings for human consumption on european markets continues to increase. although the contents of free amino acids and potentially toxic free nonprotein amino acids in these fresh and supposedly healthy seedlings is very different from those of the seeds, the crude composition is never mentioned on commercial packages. a commercial product containing seven different kinds of fresh seedlings including kamut, adzuki bean, chickpea, mungbean, pinto bean, garden pea, and le ... | 2000 | 10725139 |
| compact residual structure in lentil lectin at ph 2. | lentil lectin obtained from lens culinaris collected in the la armuña area (salamanca, spain) was examined by high-sensitivity differential scanning calorimetry, fluorimetry and measurements of circular dichroism at ph 2.0 and 7.4. at ph 2.0 the lentil lectin is not in the native state; however, at this ph it does show signs of a residual structure that breaks down upon heating. the lentil lectin at ph 2 shares some similarities with what has become known as the molten globule state. the thermal ... | 2000 | 10727954 |
| a chimeric lectin formed from bauhinia purpurea lectin and lens culinaris lectin recognizes a unique carbohydrate structure. | lectins are carbohydrate-binding proteins widely used in biochemical, immunochemical, and histochemical studies. bauhinia purpurea lectin (bpa) is a leguminous lectin with an affinity for galactose and lactose. nine amino acids, dtwpntews, corresponding to the amino acid sequence from aspartic acid-135 to serine-143 in the primary structure of bpa were replaced with the corresponding amino acid residues from the mannose-binding lens culinaris lectin (lca), and the chimeric lectin obtained was ex ... | 2000 | 10731675 |
| examination of bioaffinity immobilization by precipitation of mannan and mannan-containing enzymes with legume lectins. | the interaction of four lectins from crops of the legume family with saccharomyces cerevisiae alpha-mannan, and also with two glycoenzymes containing mainly alpha-mannan moieties, has been studied. the interaction was characterized by a quantitative precipitation assay. the results of precipitation differ with respect to both quality (the point of maximum precipitation) and of the quantity (the amount of aggregated lectin and saccharide). the lectin concanavalin a [con a, from jack bean (canaval ... | 2000 | 10744960 |
| characteristic changes in carbohydrate profile in the kidneys of hereditary nephrotic mice (icgn strain). | the icr-derived glomerulonephritis (icgn) mice consist of heterozygous and homozygous groups and are considered to be a good model for human idiopathic nephrotic syndrome. to reveal changes in cell-surface carbohydrate construction, 24 lectins were applied to kidney sections of 10-, 30- and 50-week-old male heterozygous and homozygous icgn mice and age-matched male icr mice. bandeiraea simplicifolia lectin-i (bsl-i), which specifically binds to alpha-d-galactopyranosyl groups, showed positive st ... | 2000 | 10823724 |
| the oxidation and reduction reactions of bovine serum amine oxidase. a kinetic study. | the presteady-state and steady-state kinetics of bovine serum amine oxidase (bsao) were analyzed by stopped-flow transient spectroscopy. a simplified model of the catalytic cycle was found to describe the experimental data and the rate constants of the individual steps were used to calculate michaelis parameters that agree with the direct determinations. in spite of many studies on selected reactions from the catalytic cycle, this is amongst the first efforts to provide a comprehensive kinetic d ... | 2000 | 10824112 |
| immunosuppressive applications of pha and other plant mitogens. | phytohemagglutinin was prominent in the evaluation of the immunosuppressive effects of mitogenic lectins that started in 1965 and continued for two decades. basic studies elucidated the inhibitory actions of pha on humoral and cellular immune responses in mice, rats, and guinea pigs. some suppressive effects of this and other mitogens including lentil lectin and con a were demonstrated on renal, skin, pancreas, and heart allograft rejections in mice, rats, and dogs. in addition to their inherent ... | 1998 | 10850346 |
| interaction of pig kidney and lentil seedling copper-containing amine oxidases with guanidinium compounds. | the effect of guanidinium compounds on the catalytic mechanism of pig kidney and lentil seedling amine oxidases has been investigated by polarographic techniques and spectroscopy. guanidine does not inhibit the lentil enzyme and is a weak inhibitor for pig kidney amine oxidase (ki=1 mm), whereas aminoguanidine is an irreversible inhibitor of both enzymes, with a ki value of 10(-6) m. 1,4-diguanidino butane (arcaine) is a competitive inhibitor for both pig and lentil amine oxidases. amiloride is ... | 2000 | 10850957 |
| characterisation of the carbohydrate components of taenia solium metacestode glycoprotein antigens. | human neurocysticercosis is caused by taenia solium metacestodes. it usually affects the central nervous system of humans and can be confused with other brain pathologies. the lens culinaris-binding glycoproteins from this parasite have been shown to be ideal targets for the development of a highly specific immunoassay for the diagnosis of neurocysticercosis. in the present study we characterised the carbohydrates associated with five antigenic glycoproteins of t. solium metacestodes in the rang ... | 2000 | 10856502 |
| arginine and ornithine oxidation catalyzed by lentil seedling copper-amine oxidase. | the oxidation of l-ornithine and l-arginine catalyzed by lentil (lens esculenta) seedling copper-amine oxidase has been investigated by polarographic techniques, optical spectroscopy, and capillary electrophoresis. both l-ornithine and l-arginine were found to be poor substrates for lentil amine oxidase. l-ornithine was oxidized to glutamate-5-semialdehyde and ammonia, in similar manner as usual substrates. glutamate-5-semialdehyde spontaneously cyclizes to delta1-pyrroline-5-carboxylic acid. ar ... | 2000 | 10882172 |
| a monoclonal antibody that recognizes mammalian cortical granules and a 32-kilodalton protein in mouse eggs. | the fertilization-induced exocytosis of egg cortical granules (cgs) is responsible for a block to polyspermy, crucial to the viability of many species. the contents of mammalian cgs have been an elusive target for analysis because of picogram quantities of cg proteins. by using media enriched in secreted cg contents from calcium ionophore-induced eggs as an immunogen, a monoclonal antibody was raised that immunolocalized to structures in the mouse egg cortex with all the hallmarks of cgs. these ... | 2000 | 10906067 |
| differentiation of candida strains by lectin-mediated agglutination kinetics. | the lectin-mediated agglutination kinetics of candida albicans, candida tropicalis, candida glabrata, candida krusei, candida kefyr, and candida parapsilosis strains isolated from immunocompromised patients was investigated. the rate of the lectin-induced cell agglutination depends on the physiological state of the yeast cell population. therefore, the candida strains have to be cultivated and investigated under identical conditions. lentil lectin (prepared from lens culinaris), castor lectin, a ... | 2000 | 10907339 |
| lens culinaris, phaseolus vulgaris and vicia faba lectins specifically trigger il-8 production by the human colon carcinoma cell line caco-2. | cultured caco-2 cells were stimulated with lens culinaris, phaseolus vulgarisandvicia fabalectins. the production of il-1, il-6, il-8 and mcp-1 was measured by elisa and rt-pcr. il-8 production appeared to be specifically triggered upon stimulation with all three lectins used, since none of the other cytokines tested were produced. the il-8 secreted may induce the extravasation of activated neutrophils and generate tissue damage. a similar mechanism may be implicated in the lesions observed afte ... | 2000 | 10930314 |
| early activation of lipoxygenase in lentil (lens culinaris) root protoplasts by oxidative stress induces programmed cell death. | oxidative stress caused by hydrogen peroxide (h2o2) triggers the hypersensitive response of plants to pathogens. here, short pulses of h2o2 are shown to cause death of lentil (lens culinaris) root protoplasts. dead cells showed dna fragmentation and ladder formation, typical hallmarks of apoptosis (programmed cell death). dna damage was evident 12 h after the h2o2 pulse and reached a maximum 12 h later. the commitment of cells to apoptosis caused by h2o2 was characterized by an early increase of ... | 2000 | 10931190 |
| interaction of pig kidney and lentil seedling copper-containing amine oxidases with guanidinium compounds. | the effect of guanidinium compounds on the catalytic mechanism of pig kidney and lentil seedling amine oxidases has been investigated by polarographic techniques and spectroscopy. guanidine does not inhibit the lentil enzyme and is a weak inhibitor for pig kidney amine oxidase (k(i) =1 mm), whereas aminoguanidine is an irreversible inhibitor of both enzymes, with a k(i) value of 10(-6) m. 1,4-diguanidino butane (arcaine) is a competitive inhibitor for both pig and lentil amine oxidases. amilorid ... | 1999 | 10938536 |
| immunolocalization of actin in root statocytes of lens culinaris l. | lentil root statocytes show a strict structural polarity of their organelles with respect to the g vector. these cells are involved in the perception of gravity and are responsible for the orientation of the root. actin filaments take part in the positioning of their organelles and could also be involved in the transduction of the gravitropic signal. a pre-embedding immunogold silver technique was carried out with a monoclonal antibody in order to study the distribution of actin cytoskeleton in ... | 2000 | 10938808 |
| schistosoma mansoni gene gp22 encodes the tegumental antigen sm25: (1) antibodies to a predicted b-cell epitope of sm25 cross-react with other candidate vaccine worm antigens; (2) characterization of a recombinant product containing tandem-repeats of this peptide as a vaccine. | monospecific antibodies against two putative epitopes of schistosome protein encoded by gene gp22 (182 codons, no introns) were used to probe worm extracts fractionated by lentil-lectin affinity chromatography or by electrophoresis. anti-peptide-alpha (codons 70-84) exclusively identifies the n-glycanase-sensitive, 25 kda tegumental glycoprotein sm25 in the lectin-bound fraction of detergent-solubilized adult worm extract s3. in contrast, antipeptide-delta (codons 151-162) does not react with sm ... | 2000 | 10972845 |
| a comparative study of the structure of the rdna intergenic spacer of lens culinaris medik., and other legume species. | as part of a project on lentil molecular genetics, the sequence of the 18s-25s ribosomal rna gene intergenic spacer (igs) of lens culinaris medik. was determined. dna was cloned after polymerase chain reaction (pcr) amplification. the spacer of 2939 bp was composed of nonrepetitive sequences and four tandem arrays of repeated sequences, named a to d. c and d arrays were formed by the repetition of very short consensus sequences. similarity was found between lentil and other legume species, in pa ... | 2000 | 10984170 |
| lentil root statoliths reach a stable state in microgravity. | the kinetics of the movement of statoliths in gravity-perceiving root cap cells of lens culinaris l. and the force responsible for it have been analysed under 1 g and under microgravity conditions (s/mm-03 mission of spacehab 1996). at the beginning of the experiment in space, the amyloplasts were grouped at the distal pole of the statocytes by a root-tip-directed 1-g centrifugal acceleration. the seedlings were then placed in microgravity for increasing periods of time (13, 29, 46 or 122 min) a ... | 2000 | 10987559 |
| muscular dystrophy alters the processing of light acetylcholinesterase but not butyrylcholinesterase forms in liver of lama2(dy) mice. | in order to know whether the histopathological changes of liver, which accompany muscular dystrophy, affect the synthesis of cholinesterases, the distribution and glycosylation of acetylcholinesterase (ache) and butyrylcholinesterase (buche) forms in normal (nl) and dystrophic lama2(dy) mouse liver (dl) were investigated. about half of liver ache, and 25% of buche were released with a saline buffer (fraction s(1)), and the rest with a saline-brij 96 buffer (s(2)). abundant light (g(2)(a) and g(1 ... | 2000 | 11002295 |
| localization of the lectin reactive sites in adult and prepubertal horse testes. | the testes of prepubertal and adult horses were investigated using 10 horseradish peroxidase conjugated lectins combined with sialidase digestion and potassium hydroxide treatment, to localise the oligosaccharide sequences of glycoconjugates during spermatid maturation. in adult animals, the lectins showed a variable affinity for spermatids and sertoli cell apical extensions. soybean agglutinin (sba), peanut agglutinin (pna), ricinus communis agglutinin (rca-i) and wheat germ agglutinin (wga) bo ... | 2000 | 11020360 |
| synthesis of 9,12-dioxo-10(z)-dodecenoic acid, a new fatty acid metabolite derived from 9-hydroperoxy-10,12-octadecadienoic acid in lentil seed (lens culinaris medik.) | the previously unknown linoleic acid peroxidation product 9,12-dioxo-10(z)-decenoic acid (z5) was detected in lentil seed flour (lens culinaris medik.) by electron impact mass spectrometry (ei-ms) after derivatization with pentafluorobenzyl-hydroxylamine-hydrochloride, methylation of acidic groups with diazomethane, and protection of hydroxylic groups with n-methyl-n-trimethylsilyl-trifluoroacetamide. the structure of the natural product was confirmed by synthesis of z5, 9,12-dioxo-l0(e)-decenoi ... | 2000 | 11026615 |
| [chemical composition and mineral content of legumes and cereals produced in northwestern argentina]. | different varieties of bean (phaseolus vulgaris), corn (zea mayz), lentil (lens sp), soya (glycine max) and wheat (tricticum sp) were analyzed in order to obtaining data of chemical composition and content of minerals to contribute to the international food data system infoods in the elaboration of regional food composition tables and to evaluate the quality of grains. the selected varieties were the ones produced in the northwestern region of argentina. the beans were divided in five groups, ac ... | 2000 | 11048594 |
| gentamicin traffics rapidly and directly to the golgi complex in llc-pk(1) cells. | to study the intracellular mechanisms of aminoglycoside toxicity, we used a 1:1 fluorescent conjugate of texas red and gentamicin (trg) to quantify early uptake dynamics in renal epithelial (llc-pk(1)) cells. utilizing a protocol that quenches trg fluorescence from lysosomes, the bulk of intracellular accumulation, we determined a portion rapidly trafficked directly to the golgi complex when identified by a fitc-conjugated lectin from lens culinaris agglutinin (lca). a kinetic study over 120 min ... | 2000 | 11053049 |
| lentil seed aquaporins form a hetero-oligomer which is phosphorylated by a mg(2+)-dependent and ca(2+)-regulated kinase. | in plants, aquaporins regulate the water flow through membranes during growth, development and stress responses. we have isolated two isoforms of the aquaporin family from the protein-storage vacuoles of lentil (lens culinaris med.) seeds. chemical cross-linking experiments showed that both isoforms belong to the same oligomer in the membrane and are phosphorylated by a membrane-bound protein kinase. we assigned the kinase activity to a 52 kda protein that is magnesium-dependent and calcium-regu ... | 2000 | 11062071 |
| purification and characterization of two voltage-dependent anion channel isoforms from plant seeds. | mitochondria were isolated from imbibed seeds of lentil (lens culinaris) and phaseolus vulgaris. we copurified two voltage-dependent anion channel from detergent solubilized mitochondria in a single purification step using hydroxyapatite. the two isoforms from p. vulgaris were separated by chromatofocusing chromatography in 4 m urea without any loss of channel activity. channel activity of each isoform was characterized upon reconstitution into diphytanoyl phosphatidylcholine planar lipid bilaye ... | 2000 | 11080295 |
| quantitative analysis of flavan-3-ols in spanish foodstuffs and beverages. | an hplc method, using detection after postcolumn derivatization with p-dimethylaminocynnamaldehyde (dmaca), was developed for the quantitative analysis of individual flavanols in food. this method was applied to flavanol determination in 56 different kinds of spanish food products, including fruit, vegetables, legumes, beverages (cider, coffee, beer, tea, and wine), and chocolate. the determined compounds corresponded to the catechins and proanthocyanidin dimers and trimers usually present in fo ... | 2000 | 11087482 |