Publications

the effect of dietary fat inclusion on growth, carcass characteristics, and chemical composition of rabbits.	two hundred ten californian x new zealand white rabbits were used to study the influence of diet and slaughter weight (2.0, 2.25, and 2.5 kg) on feed intake, growth, feed efficiency, carcass characteristics, and body chemical composition. seven diets were formulated to contain 24% adf: diet c (control) had no added fat; diets t, o, and s contained 3% of added beef tallow, oleins, and soybean oil, respectively; diets tws, ows, and sws contained the same level and types of fat plus 18% heated whol ...	1996	8880410
effects of dietary fat with or without nicotinic acid on nutrient flow to the duodenum of dairy cows.	four holstein cows, fitted with ruminal and duodenal cannulas, were utilized in a 4 x 4 latin square design to investigate the effects of supplementing nicotinic acid to diets that contained 35% alfalfa haylage, 15% corn silage, and either 50% of a low fat concentrate or 10% whole raw soybeans and 40% of a high fat concentrate containing tallow. treatments in a 2 x 2 arrangement were 1) low fat, no supplemental nicotinic acid; 2) low fat, 12 g/d of nicotinic acid; 3) high fat, no supplemental ni ...	1996	8880465
bean bag allergy revisited: a case of allergy to inhaled soybean dust.	soybean is a common food allergen. it can cause type 1 allergy (ige-mediated) symptoms from its ingestion, as well as from inhalation of its dust.	1996	8885807
genepool variation in genus glycine subgenus soja revealed by polymorphic nuclear and chloroplast microsatellites.	a combination of nuclear and chloroplast simple sequence repeats (ssrs) have been used to investigate the levels and pattern of variability detected in glycine max and g. soja genotypes. based on the analysis of 700 soybean genotypes with 115 restriction fragment length polymorphism (rflp) probes, 12 accessions were identified that represent 92% of the allelic variability detected in this genepool. these 12 core genotypes together with a sample of g. max and g. soja accessions were evaluated wit ...	1996	8889540
molecular cloning of a human cdna encoding putative cysteine protease (prsc1) and its chromosome assignment to 14q32.1.	we have isolated a novel human cdna encoding a protein of 433 amino acids which shows 40% sequence identity to a hemoglobinase of schistosoma japonicum, one of the cysteine proteases in the pathway by which trematodes degrade host-cell globin. it also has 36% identity to a cysteine protease of the soybean glycine max that processes proproteins of various vacuolar proteins into mature form after translation. considering the sequence similarities among these proteins in such widely divergent speci ...	1996	8893817
sphingomyelin consumption suppresses aberrant colonic crypt foci and increases the proportion of adenomas versus adenocarcinomas in cf1 mice treated with 1,2-dimethylhydrazine: implications for dietary sphingolipids and colon carcinogenesis.	sphingolipids are hydrolyzed in the gastrointestinal tract to ceramide, sphingosine, and other metabolites that can modulate cell growth, differentiation, and apoptosis. to characterize the effects of dietary sphingolipids on colon carcinogenesis, female cf1 mice were administered 1,2-dimethylhydrazine and then fed an essentially sphingolipid-free diet supplemented with 0 to 0.1% (w/w) sphingomyelin (sm) purified from milk. as was found in a previous pilot study (d. l. dillehay et al., j. nutr., ...	1996	8895747
limited proteolysis of beta-conglycinin and glycinin, the 7s and 11s storage globulins from soybean [glycine max (l.) merr.]. structural and evolutionary implications.	the g2 (a2b1a) glycinin subunit from soybean (glycine max l. merr.) was purified and renatured to the homohexameric holoprotein. this protein along with purified beta-conglycinin were subjected to limited proteolysis by trypsin. the generated polypeptide fragments were separated via sds/page and the amino acid sequence of the n-terminals was determined. four cleavage points were detected in the alpha-chain a2 of glycinin as well as in the alpha'-chain of beta-conglycinin. from the known three-di ...	1996	8898910
roasted soybeans, blood meal, and tallow as sources of fat and ruminally undegradable protein in the diets of lactating cows.	the effect of roasted soybeans, blood meal, and tallow as sources of fat and rup for lactating dairy cows was studied. forty-five cows, blocked by age, calving date, and milk yield during the previous lactation, were assigned randomly to the following treatments (ingredient in the dm, rup as a percentage of cp, and fat in the dm, respectively): 1) soybean meal (16, 35, and 3.2%), 2) whole roasted soybeans (18, 40, and 6.2%), 3) ground roasted soybeans (18, 40, and 6.2%), 4) blood meal (2.7, 40, ...	1996	8899527
seasonal effects of supplemental fat or undegradable protein on the growth and metabolism of holstein calves.	one summer (heat stress) and one winter (neutral conditions) trial were conducted to assess the effects of varied amounts of rup and fat on the growth and metabolic responses of calves. in each trial, 190-kg holstein calves (n = 45) were fed a basal diet of corn and cottonseed hulls supplemented with soybean meal (control) or supplemented with raw or roasted whole soybeans or the basal ration plus prilled hydrogenated tallow either with or without a mixture of feather meal and blood meal. prille ...	1996	8899528
[cloning, sequencing and characterizing repetitive dna of glycine max].	one satellite dna family, cloned from glycine max, was sequenced and chracterized. the unit of the repetitive sequences was 91bp. they concentrated on the arms of chromosomes m2 and m11, and spreaded on whole chromosomes sm7 and m12. southern blot analysis with 18 acessions, belonged to subgenus glycine and subgenus soj a of genus glycine, showed that the repetitive sequences were specific to subgenus soj a. it was subgenus specific repetitive sequence. this result, from another aspact, supporte ...	1995	8900841
in vitro determination of ruminal protein degradation using freeze-stored ruminal microorganisms.	mixed ruminal microorganisms were harvested from a lactating dairy cow and preserved frozen or lyophilized. fermentation characteristics of freshly strained ruminal fluid, frozen microorganisms, or lyophilized microorganisms were evaluated during a 24-h pre-incubation and a 4-h incubation with test proteins. differences observed during the first 4 to 6 h in total amino acid concentration, optical density, ph and vfa concentrations, acetate:propionate ratio, and lactate concentration largely disa ...	1996	8904719
post-fertilization changes in the zona pellucida glycoproteins of rat eggs.	the zona pellucida (zp) is the extracellular coat surrounding the mammalian egg. numerous evidence supports the role of zp carbohydrate residues as the specific sperm receptors. in this study we used lectins to study different distribution patterns of carbohydrate residues in the rat zp, and to follow changes at fertilization. zp were collected from follicular, ovulated, and fertilized eggs, incubated with one of 11 different biotin-labeled lectins, followed by avidin-fluorescein isothiocyanate ...	1996	8911967
purification and molecular analysis of an extracellular gamma-glutamyl hydrolase present in young tissues of the soybean plant.	a polypeptide present in intercellular wash fluids of young leaves of glycine max has been purified to electrophoretic homogeneity. the protein has been identified as gamma-glutamyl hydrolase (ggh) based on the shared homology with a recently cloned cdna from rat. the enzyme is present within the extracellular space of young leaves and a portion is bound to the cell wall. northern and western analysis confirm that this polypeptide is expressed only in young (1-15 d old) leaf, stem and root tissu ...	1996	8912628
roasted soybeans and an estrogenic growth promoter affect growth hormone status and performance of beef steers.	this study investigated the effects of a roasted soybean (rsb)-supplemented diet and an estrogen implant (syn; synovex-s ear implant, 20 mg estradiol benzoate plus 200 mg progesterone) in young crossbred beef steers on their performance and plasma growth hormone (gh) response to challenge injections of thyrotropin-releasing hormone (trh) + gh-releasing hormone (ghrh). twenty individually fed steers (body weight 255 +/- 5 kg) were assigned to the following treatments: 1) no syn and fed a soybean ...	1996	8914961
the activity of a blood type b specific exoglycosidase from glycine max.	soluble antigens, enzyme-linked immunosorbent assays (elisa), and cell suspension assays were used to study the blood group b activity of glycine max (soybean) alpha-d-galactosidase. the enzyme readily hydrolyzed the terminal alpha-d-galactosyl of the b antigen under a variety of conditions, converting it to h antigen. conversion of the b antigen to h antigen produces blood type o which is universally transfusable. these preliminary studies are important in determining optimal conditions for enz ...	1996	8920223
soybeans versus animal sources of rumen-undergradable protein and fat for early lactation dairy cows.	fifteen multiparous holstein cows averaging 39 dim were used in a replicated 5 x 5 latin square design to compare roasted soybeans and animal by-products as supplements for increasing the rup content of lactation diets based on alfalfa. the control diet contained roasted soybeans and was formulated to meet requirements for rup. rumen-undegradable protein in the diet was increased by including additional roasted soybeans or animal by-product proteins. to achieve diets with similar amounts of fat, ...	1996	8923252
distribution of lectin binding sites in human bone marrow. identification by use of an ultrastructural postembedding technique.	the purpose of this ultrastructural study was to detect various carbohydrate residues on mature elements of the major human haematopoietic cell lines (granulopoiesis, erythropoiesis and megakaryopoiesis), sinus endothelium and plasma cells under comparable experimental conditions. marrow specimens were processed according to a modified postembedding technique with unicryl as embedding resin. a broad panel of 10 digoxigenin (dig)-conjugated lectins was applied for staining and specificity was eva ...	1996	8933737
characterization of soybean choline kinase cdnas and their expression in yeast and escherichia coli.	an expressed sequence tag from arabidopsis that displayed sequence homology to mammalian and yeast choline kinases was used to isolate choline kinase-like cdnas from soybean (glycine max l.). two distinct cdnas, designated gmck1 and gmck2, were recovered that possessed full-length reading frames, each sharing approximately 32% identity at the predicted amino acid level with the rat choline kinase. a third unique choline kinase-like cdna, gmck3, was also identified but was not full length. hetero ...	1996	8934624
cloning of an enzyme that synthesizes a key nucleotide-sugar precursor of hemicellulose biosynthesis from soybean: udp-glucose dehydrogenase.	hemicellulose is a major component of primary plant cell walls. many of the glycosyl residues found in hemicellulose are derived from the sugar precursor udp-glucuronic acid, which can be converted into udp-arabinose, udp-apiose, udp-galacturonic acid, and udp-xylose. the enzyme controlling the biosynthesis of udp-glucuronic acid, udp-glucose dehydrogenase (ec 1.1.1.22), was cloned from soybean (glycine max [l.] merr.) by an antibody screening procedure. this enzyme is surprisingly homologous to ...	1996	8938413
differential expression of soybean cysteine proteinase inhibitor genes during development and in response to wounding and methyl jasmonate.	three cysteine proteinase inhibitor cdna clones (pl1, pr1, and pn2) have been isolated from a soybean (glycine max l. merr.) embryo library. the proteins encoded by the clones are between 60 and 70% identical and contain the consensus qxvxg motif and w residue in the appropriate spatial context for interaction with the cysteine proteinase papain. l1, r1, and n2 mrnas were differentially expressed in different organs of plants (juvenile and mature) and seedlings, although n2 mrna was constitutive ...	1996	8938418
relation of vegetable, fruit, and grain consumption to colorectal adenomatous polyps.	previous studies suggest that colorectal cancer risk decreases with higher intake of vegetables, fruits, and grains. few studies, however, have examined these factors in relation to occurrence of colorectal polyps. the authors used case-control data from 488 matched pairs to evaluate associations of vegetables, fruits, and grains with polyps. subjects were southern californians aged 50-74 years who had a sigmoidoscopy in 1991-1993. diet in the year before sigmoidoscopy was measured with a food f ...	1996	8942431
induced new mutation of d1 serine-268 in soybean photosynthetic cell cultures produced atrazine resistance, increased stability of s2qb- and s3qb- states, and increased sensitivity to light stress.	we have isolated several herbicide-resistant cell lines from photosynthetic cell suspensions of soybean (glycine max) that possessed different levels of herbicide resistance, photosystem ii activity, and chlorophyll a/b ratio. we have further studied the str7 mutant, which showed the highest level of resistance to atrazine as well as a cross-resistance to 3-(3,4-dichlorophenyl)-1, 1-dimethylurea (50- and 3-fold, respectively, compared with the wild type). sequencing of the psba gene (coding for ...	1996	8972596
nitric oxide inhibits the cytochrome oxidase but not the alternative oxidase of plant mitochondria.	oxygen consumption via the cytochrome pathway in isolated soybean (glycine max [l.] merr.) cotyledon mitochondria was inhibited by nitric oxide (no) while respiration via the cyanide-insensitive alternative oxidase was not significantly affected. inhibition of cytochrome pathway activity was rapidly reversible upon depletion of the added no. no production was also detected in solutions of nano2 plus ascorbate and the extent of cytochrome pathway inhibition was dependent on the no2- concentration ...	1996	8977097
characteristics of albumin binding to opossum kidney cells and identification of potential receptors.	albumin re-absorption in the kidney proximal tubule may be pathophysiological in disease. opossum kidney (ok) cell monolayers were used to investigate the characteristics of [125i]-labelled albumin binding at 4 degrees c. two binding sites were identified, one with high affinity (kd 154.8 +/-7 mg/l) and low capacity, the other with low affinity (kd 8300 +/- 1000 mg/l) and high capacity. binding was sensitive to lectins glycine max and ulex europaeus i, but not other lectins, indicating involveme ...	1997	9000429
an extract of soy flour influences serum cholesterol and thyroid hormones in rats and hamsters.	the effects of an ethanol-acetone extract from soy flour on serum lipids and thyroid hormones were studied in rats and hamsters. in study 1, male sprague-dawley rats were fed diets containing protein from ethanol-acetone extracted isolated soy protein (isp-), nonextracted isp (isp), casein (casein-), or casein to which 0.36 mg ethanol-acetone extract/g protein was added (casein+). rats fed either isp-containing diet had lower serum total cholesterol concentrations compared with those fed either ...	1996	9001373
isolation and analysis of the soybean sga2 gene (cdna), encoding a new member of the plant g-protein family of signal transducers.	we have isolated a cdna clone from glycine max, named sga2, coding for a g alpha-subunit protein. the encoded polypeptide, sg alpha2, shows a molecular mass of 45 kda and contains most of the conserved regions involved in guanine nucleotide binding and hydrolysis. comparison at the nucleotide and amino acid sequence levels with the other plant g alpha's shows a high degree of conservation (>85% similarity). phylogenetic analysis of these plant genes with the other g alpha's from different specie ...	1996	9002626
roasted soybeans and an estrogenic growth promoter affect the thyroid status of beef steers.	we investigated the interactive effects of a roasted soybean (rsb)-supplemented diet and an estrogen ear implant [synovex-s (syn), 20 mg estradiol benzoate + 200 mg progesterone] in young beef steers on measures of thyroid status before and after challenge injections of thyrotropin-releasing hormone (trh) + growth hormone-releasing hormone (ghrh). twenty steers (body weight 255 +/- 5 kg) were assigned to the following treatments: 1) no syn and a soybean meal-supplemented diet, 2) no syn and a rs ...	1997	9039839
a 28-kilodalton pod storage protein of french bean plants. purification, characterization, and primary structure.	when french bean (phaseolus vulgaris) plants were depodded in the early stages of fruit development, relative levels of a specific protein with a relative molecular weight of 28,000 were enhanced in the young pods that formed later. the protein, designated pod storage protein (psp), was purified from extracts of newly formed pods from plants that had been previously depodded four times at intervals of 2 weeks. two-dimensional polyacrylamide gel electrophoresis showed the presence of three forms ...	1997	9046594
performance and profiles of milk fatty acids of cows fed full fat, heat-treated soybeans using various processing methods.	twenty-four multiparous holstein cows (48 d in lactation) were used in an 8-wk trial employing a completely randomized block design to determine the effect of heat treatment of full fat soybeans on the fatty acid composition of milk fat. treatments consisted of a total mixed ration supplemented with 1) ground raw, 2) extruded, 3) micronized, or 4) roasted soybeans included at 17.5% of the dry matter of the total mixed ration. dietary treatment had no effect on body weight, yields of milk or soli ...	1997	9058276
phylogenetic and genomic relationships in the genus glycine willd. based on sequences from the its region of nuclear rdna.	phylogenetic relationships among all 18 species of the genus glycine were inferred from nucleotide sequence variation in the internal transcribed spacer (its) region of nuclear ribosomal dna. pairwise sequence divergence values ranged from 0.2% (a single nucleotide) between glycine max and glycine soja to 8.6% between glycine hirticaulis and glycine falcata. the length of the its1 and its2 sequences ranged from 215 to 238 nucleotides and from 205 to 222 nucleotides, respectively, and that of 5.8 ...	1997	9061914
a novel radioimmunoassay for daidzein.	a radioimmunoassay for daidzein was established, based on polyclonal antibodies against daidzein-4'-o-(carboxymethyl)ether-bsa. the sensitivity of the assay was 0.4 pg/tube; the intra- and interassay coefficients of variation varied from 4.1 to 11.5% and from 5.6 to 21.7%, respectively, depending upon the method (direct or extraction) and concentration of daidzein in the sample. the cross reactivities with other chemically related compounds, with the exception of 4'-derivatives of daidzein, were ...	1997	9071741
nutritional value for swine of soybean meal produced by isopropyl alcohol extraction.	three experiments were conducted to compare the nutritional value of soybean meal produced by extraction with 95% isopropyl alcohol (ipa) or hexane (hex) for swine. the same batch of soybeans and the same processing equipment were used to produce both soybean meals. analyzed contents of the ipa and hex meals were, respectively: cp, 48.7, 47.0%; lysine, 3.11, 3.06%; urease, .24, .13 delta ph. in exp. 1, two soybean meals and dietary lysine at .90 or 1.25% were used in a 2 x 2 factorial arrangemen ...	1997	9078488
major allergens in soybean and clinical significance of igg4 antibodies investigated by ige- and igg4-immunoblotting with sera from soybean-sensitive patients.	soybean occasionally causes food allergy but its major allergens have not been sufficiently confirmed. the relationship between food allergy and food-specific igg4 has also not been defined.	1997	9088659
reduction of the allergenicity of soybean by treatment with proteases.	soybeans were treated with proteases to reduce allergenicity. by immunoblotting with a monoclonal antibody against a major soybean allergen (gly m bd 30k), two of eight proteases so far tested were selected to achieve a reduction in allergenicity. both antigenicity to the monoclonal antibody and allergenicity to the sera from soybean-sensitive patients proved to be markedly reduced by processing with either protease. thus, soybeans treated with an appropriate protease may possibly be supplied as ...	1996	9089483
pathogenesis and treatment of liver fibrosis in alcoholics: 1996 update.	fibrosis is a common end stage for most chronic liver diseases. it results from an imbalance between collagen production and degradation. one promising approach for prevention and treatment is the stimulation of collagenolytic processes. in nonhuman primates it was found that polyenylphosphatidylcholine (ppc), extracted from soybeans, protects against alcohol-induced fibrosis and cirrhosis and prevents the associated hepatic phosphatidylcholine (pc) depletion by increasing 18:2-containing pc spe ...	1997	9101129
soybean dapa mutations encoding lysine-insensitive dihydrodipicolinate synthase.	in plants, the rate-limiting step in the pathway for lysine synthesis is catalyzed by the enzyme dihydrodipicolinate synthase (ds), which is encoded by the dapa gene. we previously cloned the soybean (glycine max cv. century) dapa gene in escherichia coli to express functional soybean ds protein. like the wild-type soybean ds enzyme, the ds activity encoded by the cloned gene was extremely sensitive to feedback inhibition by micromolar concentrations of lysine. three mutants of the soybean dapa ...	1997	9106516
map order and linkage distances of molecular markers close to the supernodulation (nts-1) locus of soybean.	the molecular characteristics of markers in the chromosome region surrounding the supernodulation gene (nts-1) of soybean (glycine max l. merr.) were investigated in 187 f2 plants from a cross of g. max cv. bragg (nts) and g. soja pi468.397 (wild-type nodulation). rflp marker putg-132a, linked tightly (0.7+/-0.5 cm) to nts-1, was converted to a pcr marker. the polymorphism resides within a 1.72 kb psti fragment and consists of an 832 bp insertion in g. max relative to the wild progenitor g. soja ...	1997	9108287
rapid method to separate the domains of soybean lipoxygenase-1: identification of the interdomain interactions.	lipoxygenase-1 (lox1) from soybeans was cleaved with chymotrypsin (ramachandran et al., 31 (1992) 7700-7706). the domains were separated on a sephadex g-50 column by minimising domain interactions at ph 4.0. the molecular weight and apparent homogeneity of the domains were established by sds-page. the solution conformation of the 60 kda and 30 kda fragments was compared with that of native lox1. 1-anilino-8-naphthalene sulphonate (ans) binding measurements confirmed the exposure of large hydroph ...	1997	9109414
novel, highly expressed late nodulin gene (ljnod16) from lotus japonicus.	we have isolated a lotus japonicus cdna corresponding to a highly abundant, late nodule-specific rna species that encodes a polypeptide with a predicted molecular mass of 15.6 kd. the protein and its corresponding gene were designated nlj16 and ljnod16, respectively. ljnod16 was found to be expressed only in the infected cells of l. japonicus nodules. related dna sequences could be identified in the genomes of both glycine max and medicago sativa. in the latter, a homologous mrna species was det ...	1997	9112769
a blend of animal and cereal protein or fish meal as partial replacement for soybean meal in the diets of lactating holstein cows.	six replications in experiment 1 and four replications in experiment 2 of a 3 x 3 latin square arrangement of treatments were used to compare soybean meal or soybean meal partially replaced with fish meal or a protein blend for response in intake, milk yield and composition, ruminal nh3 n, blood urea, and ruminal fermentation in lactating holstein cows. the blend contained 30% corn gluten meal, 30% poultry by-products, 30% blood meal, and 10% feather meal. periods were 28 d, and the first 7 d we ...	1997	9120086
polymerase chain reaction (pcr) in the quality and safety assurance of food: detection of soya in processed meat products.	a new method for the specific and sensitive detection of soya (glycine max) in processed meat products has been developed using polymerase chain reaction (pcr) technology. the presence of soya deoxyribonucleic acid (dna) from several soya protein concentrates was determined with two pairs of specific oligonucleotides yielding a 414-bp (bp = base pair) fragment and an internal 118-bp fragment amplified from the soya lectin le1 gene. the test detected dna from textured soya protein concentrates in ...	1996	9123971
kinetic mechanism and cofactor content of soybean root nodule urate oxidase.	the kinetic mechanism of urate oxidase isolated from soybean root nodules has been determined by initial velocity kinetic studies monitoring oxygen uptake, in order to avoid potential artifacts in the spectrophotometric assay which arise from absorbance due to unidentified products of the enzymatic reaction. urate and o2 bind to the enzyme sequentially; xanthine is a competitive inhibitor versus urate and a noncompetitive inhibitor versus o2, which suggests that urate binds to the enzyme before ...	1997	9125493
expression of glycine max (soybean agglutinin) binding molecule in injured motoneurons and its specific localization in the extracellular matrix between injured neurons and microglia.	in order to identify expression of molecules that are secreted or translocated into extracellular regions after nerve injury, lectin histochemistry using 16 lectins was carried out. after unilateral hypoglossal nerve transection, only glycin max (soybean agglutinin; sba) staining was apparent in the injured hypoglossal nucleus. the sba staining was observed clearly from 3 days after the operation, and the most intense staining was observed around postoperative day 5-7. thereafter sba staining gr ...	1997	9129185
recombinant proteins in newly developed foods: identification of allergenic activity.	a number of agricultural crops are being modified for various purposes using recombinant dna technology. since transferred genes may code for proteins that are ordinarily not present, there is concern about the potential allergenicity of these new varieties. the safety evaluation of transgenic foods is relatively easy when the allergenicity of the gene source is known. recombinant allergens in genetically engineered or altered foods can be identified using traditional immunological assays such a ...	2006	9130499
[immunologic analysis of milk, hen egg, and soybean proteins in butter and margarine, and clinical assessment for availability of hypoallergenic margarine (ham)].	to determine the allergenic proteins in commercially available butter and margarine, protein fractions were extracted and immunologically analyzed against milk, hen egg, and soybean antigens. butter and 10 kinds of margarine were proved to contain these proteins in various concentrations by elisa and immunoblotting methods by use of the rabbit antisera developed against each food proteins. however, hypoallergenic margarine was found to contain no such proteins at all. using sera obtained from at ...	1996	9133334
interaction of proteases with legume seed inhibitors. molecular features.	after having found that raw black beans (phaseolus vulgaris) were toxic, while the cooked ones constitute the basic diet of the underdeveloped peoples of the world, in the sixties, our research directed by dr. jaffé, concentrated mainly around the detection and identification of the heat labile toxic factors in legume seeds. a micromethod for the detection of protease inhibitors (pi) in individual seeds was developed, for the purpose of establishing that the multiple trypsin inhibitors (ti) foun ...	1996	9137634
protein proteinase inhibitors in legume seeds--overview.	protein proteinase inhibitors are widely distributed in plant seeds, particularly in legumes. the specificity and potency of inhibition depend on defined inhibitory sites and on the animal species of the target proteinase. feeding experiments on diets containing isolated soybean trypsin inhibitors (the kunitz soybean trypsin inhibitor sti and the bowman-birk trypsinchymotrypsin inhibitor bbi) caused insignificant growth depression in rats and chicks, but induced enlargement of the pancreas in ra ...	1996	9137635
effects of processing on antinutritional factors in legumes: the soybean case.	the author recounts his personal trail of research which has ultimately led to better understanding of the factors which contribute to the poor nutritive value of unheated soybeans. among the techniques that were employed were the isolation of a lectin from raw soybeans, the use of affinity chromatography to remove the trypsin inhibitors, and the nutritional evaluation of soybean varieties which lacked the lectin or the kunitz trypsin inhibitor. based on a consideration of the results obtained b ...	1996	9137638
eating to beat breast cancer: potential role for soy supplements.	the study reviews the anticancer properties of natural isoflavones which occur in especially high concentration in soybeans. it considers the suitability of soybean products for clinical trials aiming to reduce the progression of breast cancer.	1997	9137789
inhibitory activity of soyasaponin ii on virus replication in vitro.	the antiviral activities of two saponins, soyasaponin i and ii, isolated from soybean (glycine max merrill) were studied in vitro against herpes simplex virus type 1 (hsv-1). soyasaponin ii was more potent than soyasaponin i as shown by reduction of hsv-1 production. soyasaponin ii was also found to inhibit the replication of human cytomegalovirus, influenza virus, and human immunodeficiency virus type 1. the action was not due to inhibition of virus penetration and protein synthesis, but might ...	1997	9140220
purification and characterization of a soybean hull allergen responsible for the barcelona asthma outbreaks. ii. purification and sequencing of the gly m 2 allergen.	a low mw allergen from soybean hull, gly m 1, with two isoallergens, gly m 1 a and gly m 1 b, was associated with the asthma outbreaks that occurred in cartagena, spain. using sera of asthmatic epidemic patients (aep) from barcelona, three main soybean hull allergens, two of them with mws and pis identical to those reported for gly m 1 a and gly m 1 b, were identified.	1997	9146936
[development of an intermediate moisture product for the elderly based on texturized soybeans].	in accordance with the fact the aging process is closely related to the individual nutrition, it is possible to infer that the daily diet could help and improve, to a certain degree, to the elderly well-being. based on this and making use of the food technology expertise, the development of a low cost, easily prepared and preserved product, in accordance with the taste and needs of the elderly people was considered. the objective of this project was to elaborate an intermediate moisture food (im ...	1995	9161450
curcumin and genistein, plant natural products, show synergistic inhibitory effects on the growth of human breast cancer mcf-7 cells induced by estrogenic pesticides.	curcumin and genistein are two natural products of plants obtained from curcuma longa linn (turmeric) and soybeans, respectively. both compounds when present at micromolar concentrations are able to inhibit the growth of estrogen-positive human breast mcf-7 cells induced individually or by a mixture of the pesticides endosulfane, ddt and chlordane or 17-beta estradiol. when curcumin and genistein were added together to mcf-7 cells, a synergistic effect resulting in a total inhibition of the indu ...	1997	9168916
digestible sulfur amino acid requirement of starting turkeys.	three experiments (a total of 1,020 poults) were conducted to determine the digestible sulfur amino acid (saa) requirement for female turkey poults during the starter period. poults were fed a standard corn-soybean meal diet (pc) that met or exceeded nrc recommendations (28% cp, 3,172 kcal men/kg) for 1 wk and were then randomly assigned to treatments until 22 d (experiment 2 and 3) or 23 d (experiment 1) of age. dietary treatments included the pc diet and seven or nine titrated levels of methio ...	1997	9181621
differential expression of the multigene family encoding the soybean mitochondrial alternative oxidase.	the alternative oxidase (aox) of the soybean (glycine max l.) inner mitochondrial membrane is encoded by a multigene family (aox) with three known members. here, the aox2 and aox3 primary translation products, deduced for cdna analysis, were found to be 38.1 and 36.4 kd, respectively. direct n-terminal sequencing of partially purified aox from cotyledons demonstrates that the mature proteins are 31.8 and 31.6 kd, respectively, implying that processing occurs upon import of these proteins into th ...	1997	9193084
soybean root nodule acid phosphatase.	acid phosphatases are ubiquitous enzymes that exhibit activity against a variety of substrates in vitro, although little is known about their intracellular function. in this study, we report the isolation, characterization, and partial sequence of the major acid phosphatase from soybean (glycine max l.) root nodules. the phosphatase was purified predominantly as a heterodimer with subunits of 28 and 31 kd; homodimers of both subunits were also observed and exhibited phosphatase activity. in addi ...	1997	9193092
soybean (glycine max) agglutinin binds to corneal endothelial cells during wound repair and alters their microfilament pattern.	in the present study we have examined soybean (glycine max) agglutinin (sba) binding to cells of the rat corneal endothelium during wound repair. circular transcorneal freeze injuries were given to the endothelia and the tissues were organ cultured at 37 degrees c in basal media eagle with 10% serum for up to 72 hrs. sba failed to bind to the surface of non-injured corneal endothelium, but strongly bound to cells involved in the wound repair process. punctate surface binding was detected 24 hrs. ...	1997	9193787
evaluation of selected food characteristics of three advanced lines of nigerian soybean (glycine max (l.) merr.).	the seeds of three promising advanced lines of soybeans (tgx 923-2en, tgx 1019-2en and tgx 1497-1d) which were part of a larger collection evaluated in agronomic field trials in nigeria were selected for characterization of physicochemical properties, chemical composition, water absorption, cooking time and cooked texture as a function of soaking and cooking. seed density, leached solids, swelling capacity and seed coat percentage were within a range of 1.15 to 1.26 g per ml, 1.00 to 1.26 g per ...	1997	9198111
effect of ph and temperature on the immunogenicity of glycinin (glycine max l.).	sensitive immunologic techniques for the detection of alterations that occur in protein antigens were used to evaluate the immunogenicity of soybean glycinin after isolation, heat denaturation and ph alteration. the objective was to determine the effect of these agents on the immunogenic ability of this protein fraction. immunologic assays performed on heat-denatured glycinin up to 80 degrees c in the presence of antinative glycinin serum demonstrated that glycinin retains its immunogenic proper ...	1997	9198116
response of lactating cows to methionine or methionine plus lysine added to high protein diets based on alfalfa and heated soybeans.	lactation diets based on wilted alfalfa silage and heated whole soybeans are common in the midwestern us. we examined the milk production response of multiparous holstein cows to the addition of ruminally protected methionine at two percentages to a basal total mixed ration. an additional total mixed ration included both methionine and lysine supplementation. sixteen holstein cows in early lactation were used in a replicated 4 x 4 latin square design with 21-d periods. milk production, milk comp ...	1997	9201591
an application of nmr microimaging to investigate nitrogen fixing root nodules.	various techniques to obtain high-resolution nmr images (voxel size down to 39 x 39 x 250 microns) of nitrogen-fixing root nodules from soybean [glycine max (merr.)] and peanut (arachis hypogaea l.) are compared. we describe the artefacts arising from changes in the magnetic susceptibility throughout the sample and how these can be minimised. a series of t1 (tr = 220 to 3020 ms) and t2 (te = 9.3 to 33.6 ms) weighted images are presented. from these it has been possible to locate the oxygen diffu ...	1997	9201685
amino acid composition, available lysine content and in vitro protein digestibility of selected tropical crop seeds.	as the search for alternative sources of food to alleviate hunger continues, this study was undertaken to determine nitrogen and amino acid content, chemical score, protein digestibility corrected amino acid score, available lysine and in vitro digestibility of 8 lesser known, wild tropical seeds, gathered in nigeria. results were contrasted with a tropical soybean variety (glycine max, tgx 1660-15f). the investigated seeds were millettia thonningii, gliricidia sepium, lonchocarpus sericeus, alb ...	1997	9201749
multi-year monitoring of canadian grains and grain-based foods for trichothecenes and zearalenone.	monitoring of canadian grain crops and foods by the health protection branch for deoxynivalenol (don, vomitoxin) has been undertaken every year since 1980, when it was found in ontario soft wheat for the first time (in the 1979 and 1980 crops). contamination of this wheat crop has varied, with 22-100% incidences in all but 1 year and up to 0.75 micrograms/g for the annual means of positive samples. the canadian guideline for don is 2 micrograms/g in uncleaned soft wheat. western canadian hard wh ...	1997	9205561
the ribosomal protein p0 of soybean (glycine max l. merr.) has antigenic cross-reactivity to soybean seed lectin.	soybean (glycine max l. merr.) mutants lacking the ability to produce the lectin normally found in soybean seeds (sbl) are designated le-. a protein of higher molecular weight that cross-reacts with antibodies raised to sbl was found at nearly equivalent levels in roots, hypocotyls, and leaves, and at lower levels in cotyledons and dry seeds of both le+ and le- soybean cultivars. earlier work suggested that this protein was a novel lectin. clones isolated from a le- soybean root cdna library pro ...	1997	9207845
identification of cdnas encoding sterol methyl-transferases involved in the second methylation step of plant sterol biosynthesis.	two methyl transfers are involved in the course of plant sterol biosynthesis and responsible for the formation of 24-alkyl sterols (mainly 24-ethyl sterols) which play major roles in plant growth and development. the first methyl transfer applies to cycloartenol, the second one to 24-methylene lophenol. five cdna clones encoding two arabidopsis thaliana, two nicotiana tabacum and one ricinus communis s-adenosyl-l-methionine (adomet) sterol methyltransferases (smt) were isolated. the deduced amin ...	1997	9208946
urinary isoflavonoid excretion in humans is dose dependent at low to moderate levels of soy-protein consumption.	soybeans contain isoflavones, which have been associated with many health benefits, including decreased cancer risk. the purpose of our study was to measure urinary isoflavonoid excretion in response to daily consumption of soy that contained 0-36 mg isoflavones--a lower range than used in previous studies--and to compare urinary isoflavonoid excretion between equol excreters and nonexcreters. fourteen men and women aged 20-40 y participated in the study. half of the subjects were identified pre ...	1997	9209168
exposure of infants to phyto-oestrogens from soy-based infant formula.	the isoflavones genistein, daidzein, and their glycosides, found in high concentrations in soybeans and soy-protein foods, may have beneficial effects in the prevention or treatment of many hormone-dependent diseases. because these bioactive phyto-oestrogens possess a wide range of hormonal and non-hormonal activities, it has been suggested that adverse effects may occur in infants fed soy-based formulas.	1997	9217716
secretion of active recombinant phytase from soybean cell-suspension cultures.	phytase, an enzyme that degrades the phosphorus storage compound phytate, has the potential to enhance phosphorus availability in animal diets when engineered into soybean (glycine max) seeds. the phytase gene from aspergillus niger was inserted into soybean transformation plasmids under control of constitutive and seed-specific promoters, with and without a plant signal sequence. suspension cultures were used to confirm phytase expression in soybean cells. phytase mrna was observed in cultures ...	1997	9232886
dietary phytoestrogens.	broadly defined, phytoestrogens include isoflavones, coumestans, and lignans. a number of these compounds have been identified in fruits, vegetables, and whole grains commonly consumed by humans. soybeans, clover and alfalfa sprouts, and oilseeds (such as flaxseed) are the most significant dietary sources of isoflavones, coumestans, and lignans, respectively. studies in humans, animals, and cell culture systems suggest that dietary phytoestrogens play an important role in prevention of menopausa ...	1997	9240932
response of lactating cows to supplemental unsaturated fat and niacin.	responses to supplemental unsaturated fat or niacin in a 2 x 2 factorial arrangement were evaluated with 16 lactating holstein cows in a replicated latin square with 4-wk periods. total mixed diets contained (dry matter basis) 25% alfalfa hay, 25% corn silage, and 50% concentrate mixture. diets were the control or the control supplemented with unsaturated fat (3% of dry matter of the total mixed diet from extruded soybeans, which replaced soybean meal and portions of the corn and barley in other ...	1997	9241594
whole cottonseeds or a combination of soybeans and soybeans hulls in the diets of lactating dairy cows.	whole raw soybeans and soybean hulls were evaluated as a dietary replacement for whole cottonseed as determined by rumination and total chewing activity, milk fat percentage, and efficiency of 4% fat-corrected milk (fcm) production. twenty-six holstein dairy cows (14 multiparous; 35 +/- 12 d of lactation) were assigned randomly to one of two total mixed rations (tmr) for 15 wk. the whole cottonseed tmr and the soybean plus soybean hull tmr contained 40% of a 45:55 mixture of alfalfa and corn sil ...	1997	9241596
purification and antifungal activity of a basic 34 kda beta-1,3-glucanase from soybean hypocotyls inoculated with phytophthora sojae f. sp. glycines.	inoculation of soybean (glycine max l. cv. jangyup) hypocotyls with phytophthora sojae f. sp. glycines results in a marked accumulation of some pathogenesis-related (pr) proteins. a basic beta-1,3-glucanase (34 kda) was purified from soybean hypocotyls infected by an incompatible race of p. sojae f. sp. glycines using cm-cellulose cation exchange chromatography and bio-gel p-60 gel filtration. the purified soybean beta-1,3-glucanase cross-reacted with polyclonal antibody raised against a tomato ...	1997	9264030
association of soy and fiber consumption with the risk of endometrial cancer.	the authors conducted a case-control study among the multi-ethnic population of hawaii to examine the role of dietary soy, fiber, and related foods and nutrients on the risk of endometrial cancer. endometrial cancer cases (n = 332) diagnosed between 1985 and 1993 were identified from the five main ethnic groups in the state (japanese, caucasian, native hawaiian, filipino, and chinese) through the rapid-reporting system of the hawaii tumor registry. population controls (n = 511) were selected ran ...	1997	9270408
particle size of roasted soybeans and the effect on milk production of dairy cows.	fifteen cows were used in an experiment with a 5 x 5 replicated latin square design to quantify the effect of particle size of roasted soybeans on milk production and fecal excretion of soybeans. the five experimental periods were each 2 wk long. diets contained (percentage of dry matter) 33% alfalfa silage, 17% corn silage, 30.6% high moisture ear corn, 18% soybeans, and 1.4% mineral supplement. the five dietary treatments included raw whole soybeans or roasted soybeans in four particle sizes ( ...	1997	9276812
the major intrinsic protein family of arabidopsis has 23 members that form three distinct groups with functional aquaporins in each group.	aquaporins, proteins that enhance the permeability of biological membranes to water, are widely distributed in living organisms. they are 26- to 29-kd proteins that belong to the major intrinsic protein (mip) family of channels. by searching the arabidopsis thaliana expressed sequence tag database and by using the polymerase chain reaction with oligonucleotides to conserved plant aquaporin domains, we identified 23 expressed arabidopsis mip genes. eight of these had been previously identified as ...	1997	9276952
structural characterization of the k antigens from rhizobium fredii usda257: evidence for a common structural motif, with strain-specific variation, in the capsular polysaccharides of rhizobium spp.	rhizobium fredii participates in a nitrogen-fixing symbiosis with soybeans, in a strain-cultivar-specific interaction, and past studies have shown that the cell surface and extracellular polysaccharides of rhizobia function in the infection process that leads to symbiosis. the structural analysis of the capsular polysaccharides (k antigens) from strain usda257 was performed in this study. the k antigens were extracted from cultured cells with hot phenol-water and purified by size exclusion chrom ...	1997	9286989
purification and characterization of the acid soluble 26-kda polypeptide from soybean seeds.	whey proteins from soybean seeds of japanese varieties were analyzed by sds-polyacrylamide gel electrophoresis (sds-page). among 11 varieties of soybean, three green and one black soybeans lacked a 26-kda band that was found in all yellow soybeans. in this paper, the 26-kda protein was named as26k (acid soluble 26-kda protein) temporarily. the as26k protein was purified from glycine max cv. nattosyoryu, which is yellow soybean, through four purification steps: 30-35% saturated ammonium sulfate f ...	1997	9301109
molecular cloning and characterization of a cdna encoding putative phospholipid hydroperoxide glutathione peroxidase from spinach.	a cdna encoding spinach putative phospholipid hydroperoxide glutathione peroxidase (phgpx) was cloned and sequenced. the cdna included an open reading frame that encoded a polypeptide of 171 amino acid residues. the deduced amino acid sequence showed about 77 and 50% similarity to plant putative phgpxs and mammalian phgpxs, respectively. pcr product with the same size as that of the spinach putative phgpx were obtained from maize, soybeans, and arabidopsis, suggesting the expression of putative ...	1997	9301122
genistein inhibits growth of b16 melanoma cells in vivo and in vitro and promotes differentiation in vitro.	consumption of soy products has been linked to a reduced mortality and morbidity from a number of cancers. genistein, one of the principal soy isoflavones, has been shown to inhibit the growth of a number of tumour cell lines in vitro; however, a role of genistein in retarding tumour growth in vivo is less well documented. in this study, in addition to examining the effects of genistein on the growth of murine b16 melanoma cells in vitro, we have examined the effects of feeding a genistein-rich ...	1997	9311605
[effect of glycine max lectin on the interaction of prothrombin and erythrocytes].	native porcine erythrocytes do not initiate blood coagulation, though even the weak association (kd = 4,25 +/- 9,35 microm) of prothrombin with their surface which is limited to the projection of two phospholipid polar head groups onto the external cell membrane, exerts a slight but authentic influence on the acceleration of blood clotting. there are 11.9 x 10(7) sites for 125i-prothrombin on one erythrocytes. at physiological concentration of prothrombin the surface of erythrocytes is far from ...	1997	9312937
response of lactating dairy cows to mechanically processed whole cottonseed.	twenty-four lactating holstein cows averaging 142 +/- 34 d in milk were used in a replicated 4 x 4 latin square trial to determine the effect of processed whole cottonseed on milk yield and nutrient digestibility. fifteen percent of the dry matter content of the experimental diets consisted of whole cottonseed, roasted whole cottonseed, a roasted and pelleted blend of whole cottonseed and soybean meal, or an extruded blend of whole cottonseed and soybeans. apparent digestibilities of dry matter ...	1997	9313148
the effects of dietary soybean versus skim milk protein on plasma and hepatic concentrations of zinc in veal calves.	we assessed the zinc status of veal calves that were fed milk replacers containing either skim milk protein as the sole source of protein or a mixture of skim milk protein and soybean protein. after the milk replacers had been fed for 26 wk, mean body weight gain was 3 kg lower for calves fed the skim milk plus soybean proteins; this decrease was not significant. inclusion of dietary protein from soybeans versus milk protein alone reduced plasma concentrations of zinc by 43% and reduced hepatic ...	1997	9313159
toxicity identification of poultry litter aqueous leachate.	poultry litter is a mixture of excreta, feed, feathers, and bedding material. poultry litter is useful as a fertilizer due to the presence of nitrogen, phosphorus, and potassium. litter is commonly used for growing corn, soybeans, potatoes, tomatoes, leafy vegetables, and cover crops. the aqueous leachate of poultry litter has been shown to exhibit toxicity to a variety of indicator organisms. the objective of this research was to identify classes of toxicants in the litter aqueous leachate. the ...	1997	9316111
phytochemicals: guardians of our health.	consuming a diet rich in plant foods will provide a milieu of phytochemicals, nonnutritive substances in plants that possess health-protective benefits. nuts, whole grains, fruits, and vegetables contain an abundance of phenolic compounds, terpenoids, pigments, and other natural antioxidants that have been associated with protection from and/or treatment of chronic disease such as heart disease, cancer, diabetes, and hypertension as well as other medical conditions. the foods and herbs with the ...	1997	9336591
polyamine content of ordinary foodstuffs and various fermented foods.	soybeans, tea leaves, and mushrooms were conspicuously rich in spermidine, while oranges contained a large amount of putrescine. among the fermented foods, soy sauces were rich in putrescine and histamine, while japanese sake contained plenty of agmatine. these polyamines are thought to be produced from amino acids during fermentation with amino acid decarboxylases formed by the micro-organisms.	1997	9339564
production of a baculovirus-derived gp50 protein and utilization in a competitive enzyme-linked immunosorbent assay for the serodiagnosis of pseudorabies virus.	the pseudorabies virus (prv) gp50 envelope glycoprotein gene was cloned and expressed in a recombinant baculovirus. an anti-gp50 mab (1842) recognized a protein of approximately 40 kda in immunoblotting assays from infected insect cell lysates, while this product was not present in cells infected with wild-type baculovirus. the recombinant protein was purified by lectin affinity chromatography, utilizing lectins specific for o-linked oligosaccharides (artocarpus integrifolia and glycine max). co ...	1997	9342453
cell-surface carbohydrates of entamoeba invadens.	cell-surface carbohydrates of entamoeba invadens trophozoites were analyzed using (a) a panel of highly purified lectins specific for molecules containing n-acetylglucosamine or sialic acid, n-acetylgalactosamine, galactose, mannose-like residues, and fucose; (b) escherichia coli k-12 with mannose-sensitive fimbria; (c) enzymatic digestion; and (d) scanning electron microscopy. the presence of galactose (d-gal) and n-acetylgalactosamine (d-galnac) was detected in the amoeba. previous trypsinizat ...	1997	9342747
soy feeding induces phase ii enzymes in rat tissues.	the ability of soy to induce phase ii detoxification enzymes was evaluated in male sprague-dawley rats. soybeans contain biologically active compounds that are known inducers of phase ii enzyme activity. rats were fed soy flour (sf) or soy protein isolate (spi) to provide 75% of total protein as soy. rats were given free access to food for one- and two-week periods before enzyme activity was compared with that of casein control groups (ain-93g). hepatic glutathione s-transferase (gst) activity w ...	1997	9343836
soybeans & the kingdom of god.		1996	9362763
the str120 satellite dna of soybean: organization, evolution and chromosomal specificity.	a highly repeated dna sequence family, str120, with tandemly arranged repetitive units (monomers) of approximately 120bp, has been identified in soybean [glycine max (l.) merr.]. five related clones showing tandem repeats of a 120-bp-long monomer were isolated from a soybean genomic library. results of southern blotting experiments using three of the clones as probes onto genomic dna digested with different restriction enzymes were in agreement with a tandem arrangement of these sequences in the ...	1997	9364938
accumulation of phytate in vegetable-type soybean genotypes harvested at four developmental stages.	a total of 17 vegetable-type soybean (glycine max [l.] merr) genotypes were planted in four-row plots arranged in a randomized complete block design in 1988 and 1989 at petersburg, virginia. each genotype was harvested at four developmental stages and evaluated for phytate content. highly significant differences for phytate content were observed among the stages of harvest and genotypes, and there was an interaction between genotype x stage of harvest. the significant differences observed for ph ...	1997	9373869
assessment of postruminal amino acid digestibility of roasted and extruded whole soybeans with the precision-fed rooster assay.	the objectives of these studies were to predict the effects of roasting and extrusion temperatures of whole soybeans (sb) on intestinal protein digestibility in cattle. intestinal digestibility was assessed with a two-stage in vitro or in situ ruminal incubation/precision-fed cecectomized rooster bioassay. in exp. 1, whole sb (raw sb or sb roasted to 141, 149, or 157 degrees c exit temperature from a commercial roaster and steeped for 30 min) were incubated in strained ruminal fluid and mcdougal ...	1997	9374321
[comparative study of the composition and nutritional value of the seeds and protein concentrations in legumes].	the nutritional properties of three legumes: pea (pisum sativum), faba bean (vicia faba) and soya (glycine max) have been characterized. from these seeds, protein concentrates were elaborated by wet processing and two different procedures of drying were followed (freeze-drying and alcohol washing). the composition and content of several antinutritional factors (phytates, tannins, trypsin inhibitors and lectins) were assessed in all of them. also some functional properties regarding their potenti ...	1995	9382685
effect of dietary supplementation of soybeans on experimental metastasis of melanoma cells in mice.	the purpose of the present study was to determine the effect of dietary supplementation of soybean protein isolate (spi) on experimental metastasis of b16bl6 murine melanoma cells in c57bl/6 mice. four groups of mice were fed a basal ain-93g diet or the basal diet supplemented with 10%, 15%, or 20% spi for two weeks before and after the intravenous injection of 0.75 x 10(5) cells. at necropsy the number of tumors that developed in the lungs and their cross-sectional area were determined, and tum ...	1997	9383777
a comparative survey of leguminous plants as sources of the isoflavones, genistein and daidzein: implications for human nutrition and health.	over 80 taxa of mostly agriculturally important legumes were surveyed as sources of the metabolites, genistein and daidzein. remarkably high concentrations (over 2 g.kg-1 dry weight) of the anticancer metabolite, genistein, were found in the leaves of psoralea corylifolia (indian bread root). all other legumes, with the exception of fermented soybean miso, had genistein levels < 400 mg.kg-1 dry weight. concentrations of over 1 g.kg-1 dry weight and 0.95 g.kg-1 dry weight of the anticancer metabo ...	1997	9395689
x-ray crystallographic studies of unique cross-linked lattices between four isomeric biantennary oligosaccharides and soybean agglutinin.	soybean agglutinin (sba) (glycine max) is a tetrameric galnac/gal-specific lectin which forms unique cross-linked complexes with a series of naturally occurring and synthetic multiantennary carbohydrates with terminal galnac or gal residues [gupta et al. (1994) biochemistry 33, 7495-7504]. we recently reported the x-ray crystal structure of sba cross-linked with a biantennary analog of the blood group i carbohydrate antigen [dessen et al. (1995) biochemistry 34, 4933-4942]. in order to determine ...	1997	9398234
purification and characterization of arginine decarboxylase from soybean (glycine max) hypocotyls.	arginine decarboxylase (ec 4.1.1.19) was purified from soybean, glycine max, hypocotyls by a procedure which includes ammonium sulfate fractionation, acetone precipitation, gel filtration chromatography, and affinity chromatography. using this procedure, adc was purified to one band in non-denaturing page. the purified adc has an m(r) of 240 kda based on gel filtration chromatography and is a trimer of identical subunits which has an estimated m(r) of 74 kda based on sds-page. adc is active betw ...	1997	9399439
differential expression of adc mrna during development and upon acid stress in soybean (glycine max) hypocotyls.	arginine decarboxylase (adc) is one of the key enzymes in the biosynthesis of putrescine in plants. the regulation of its activity depends on the physiological condition, developmental stage, and type of tissue. we have cloned adc cdna from soybean (glycine max) hypocotyls to understand the regulation mechanisms of this enzyme activity. using the cdna clone, we examined the relationship between changes in the adc activity and the level of adc mrna during development, in different tissues, and up ...	1997	9399440
structural analysis of n-glycans of storage glycoproteins in soybean (glycine max. l) seed.	the structures of n-linked sugar chains (n-glycans) of storage glycoproteins in soybean seeds have been identified. eight pyridylaminated (pa-) n-linked sugar chains were derived and purified from hydrazinolysates of the storage glycoproteins by reverse-phase hplc and size-fractionation hplc. the structures of the pa-sugar chains purified were first identified by two-dimensional pa-sugar chain mapping and ion-spray mass analysis, considering the results of sugar composition analysis or sequentia ...	1997	9404066
dietary soybeans extruded at different temperatures: milk composition and in situ fatty acid reactions.	twenty-four multiparous holstein cows, 4 of which were ruminally fistulated, were assigned to one of four diets containing full-fat soybeans, either raw or extruded at 120, 130, or 140 degrees c. our hypothesis was that the extrusion of full-fat soybeans, as well as the extrusion temperature, would affect the bypass of fatty acids in the rumen and, thus, would modify the fatty acid profile of milk fat. total mixed diets containing 23.7% soybeans (percentage of dm) were fed for 8 wk. milk yield w ...	1997	9406085