Publications

[morphology of the initial neurons in the cortico-cortical connections between the auditory fields and the sensomotor region revealed by horseradish peroxidase].		1979	761499
the mechanism of indole-3-acetic acid oxidation by horseradish peroxidases.	the oxygen-consuming oxidation of indole-3-acetic acid (iaa) occurred much faster in the presence of horseradish peroxidase c (neutral isoenzyme) than in the presence of horseradish peroxidase a (acidic isoenzyme). an intermediate oxidation product of iaa was found to be a hydroperoxide species that reacted with the ferric enzymes to form compound i at second order rate constants of 6.8 x 10(3) m-1--s-1 for peroxidase a and 2.0 x 10(6) m-1--s-1 for peroxidase c at ph 4.4 the hydroperoxide concen ...	1979	762098
the organization of the electromotor nucleus and extraocular motor nuclei in the stargazer (astroscopus y-graecum).	the organization of the oculomotor and electromotor systems was examined in the stargazer, a teleost. the electromotor system in these animals is a derivative of the oculomotor system. the extraocular motor nuclei and nerves consist of approximately equal numbers of motoneurons and axons (about 100 per muscle). in contrast, electromotor axons appear to branch several times within the intracranial portion of the iiird nerve. the topographical organization of the motoneurons was examined using ret ...	1979	762265
proprioceptive reflex connections of head musculature and the mesencephalic trigeminal nucleus in the carp.	the location of the sensory cells concerned with the proprioception of respiratory and extraocular musculature in the carp was studied by retrograde transport of horseradish peroxidase. sensory cell labeling after intramuscular hrp injection was exclusively found in the trigeminal-facial-anterior lateral line ganglion complex. the muscles innervated by the trigeminal system are represented in the more rostral ganglion areas, the muscles innervated by the facial system in the more caudal ganglion ...	1979	762276
the organization of afferents to the cerebellar cortex in the cat: projections from the deep cerebellar nuclei.	the topography of the cerebellar nucleo-cortical projection was investigated in the cat by experiments employing the horseradish peroxidase (hrp) technique or by combined hrp-autoradiographic methods. the results of the hrp studies extend previous findings showing that neurons in the deep nuclei project to the cerebellar cortex in an orderly way. thus, it appears that the cortex of the vermis-proper receives projections from neurons located predominately in the fastigial nucleus. intermediate an ...	1979	762281
sources of subcortical projections to the superior colliculus in the cat.	a comprehensive search for subcortical projections to the cat superior colliculus was conducted using the retrograde horseradish peroxidase (hrp) method. over 40 different subcortical structures project to the superior colliculus. the more notable among these are grouped under the following categories. visual structures: ventral lateral geniculate nucleus, parabigeminal nucleus, pretectal area (nucleus of the optic tract, posterior pretectal nucleus, nuclei of the posterior commissure). auditory ...	1979	762286
regeneration of cerebral microvessels: a morphologic and histochemical study after local freeze-injury.	the sequential changes that occur during regeneration of cerebral microvessels was studied in young mice after a local freeze-injury. the immediate breakdown in the blood-brain barrier to horseradish peroxidase was followed by dissolution of cells in the vessel wall although the basal lamina was preserved. this residual basal lamina formed the pathway for the orderly regeneration of microvessels. three days after injury, reactive endothelial cells with prominent pinocytosis and patent interendot ...	1979	762954
a new method for the cytochemical demonstration of peroxidase for light, fluorescence and electron microscopy.	a new technique for the cytochemical demonstration of peroxidase is presented. monomeric homovanillic acid is converted by h2o2 and peroxidase into its dimeric form, which is then precipitated as a complex salt of lead and rhodamine 6g or rhodamine b. the reaction product can be visualized by conversion to lead sulphide or viewed directly under the fluorescence microscope, since it emits a red fluorescence when excited with green light. the reaction is rapid and results in good localization at t ...	1976	768373
[immunofluorescence and immunoelectron microscopic study of changes occurring in secondary reaction of popliteal lymph nodes in young and old rats].	a comparative study of the secondary reaction of popliteal lymph nodes in young and adult rats was carried out. in one group of experiments secondary reaction was induced by bovine serum albumin (bsa), in the other one by peroxidase from horseradish. antigen was administered with freund adjuvant. it was established that the index of popliteal lymph nodes in a secondary reaction induced by bsa does not change with the increasing age. the incidence of antibody-producing cells in young and adult ra ...	1976	781524
peroxidase-catalyzed halogenation.	peroxidase-catalyzed halogenation reactions have been established as being important in the biosynthesis of the hormone thyroxine and in biological defense mechanisms. recently these reactions have been recognized as valuable tools for the study of proteins as well as their arrangement in macromolecular structures. the pathways of peroxidase catalyses can be accommodated within the framework of the classical chance-george mechanism. this implies that the initial steps of the reaction invariably ...	1976	786162
distribution of dengue-2 antigens by electron immunocytochemistry.	the distribution of dengue-2 antigens was studied in infected monkey kidney cells (llc mk2) using an indirect, horseradish peroxidase-conjugated immunoglobulin technique. this procedure allowed both light and electron microscopic examination of serial-step sections of individual cells cut in a plane perpendicular to the monolayer. both virion and nonvirion antigens were identified on the plasma membrane with this technique. a diffuse cytoplasmic reaction product was also present. the intensity a ...	1976	786880
detection of endogenous immunoreactive prolactin in rat mammary epithelial cells during lactation.	the question of whether endogenous prolactin might be detectable in normally lactating rat mammary glands (lmg) was examined by light microscopic immunohistochemistry. in an initial study, sections of lmg were incubated sequentially with i) rabbit anti-rat prolactin (aprl); ii) anti-rabbit gamma globulin: peroxidase conjugate (argammag-per), prepared from goat anti-rabbit gamma globulin and horseradish peroxidase, and iii) 3,3'-diaminobenzidine mixed with h2o2(dab). use of this three-step proced ...	1976	789055
cell surface saccharides of trypanosoma lewis i. ii. lectin-mediated agglutination and fine-structure cytochemical detection of lectin-binding sites.	bloodstream (bsf) and culture forms (cf) of trypanosoma lewisi were specifically agglutinated with the plant lectins concanavalin a (con a), soybean agglutinin (sba), wheat germ agglutinin (wga), and fucose-binding protein (fbp). lectin-mediated cell agglutination was inhibited, and reversed in the presence of specific lectin-binding saccharides. cells were agglutinated randomly with all lectins suggesting a uniform distribution in the trypanosome cell surface of the lectin-binding saccharide li ...	1976	789385
characterization of macrophage migration inhibitory factor activity produced in vivo by a cell-mediated immune reaction in the guinea pig.	peritoneal fluid from the abdominal cavities of guinea pigs having delayed hypersensitivity to horseradish peroxidase (hrpo) was obtained by a lavage technique before and after i.p. challenge with antigen. macrophage migration inhibitory factor (mif) and macrophage chemotactic factor activities were measured in peritoneal fluids from each animal. chemotactic activity for macrophages was maximal 24 hr after i.p. challenge and was absent thereafter. mif activity was maximal in peritoneal fluid 24 ...	1976	794416
immuno-electron microscopic localization of immunoglobulins in amyloid fibrils of senile plaques.	amyloid fibrils in senile plaques of the brain of patients with senile dementia or alzheimer's disease combined specifically with horseradish peroxidase (hrpo)-labeled rabbit anti-human igg. light and electronmicroscopic immunoperoxidase technique was used to prove this. the fact may mean that immunological factors were involved in the pathogenesis of amyloid fibrils in the senile plaques, and probably also in that of senile dementia or alzheimer's disease.	1976	795258
[detection of antinuclear factor with horseradish peroxidase-conjugated antibody].		1976	798712
trans-epidermal elimination of immune deposits in the skin. an immunohistochemical study on immune deposits using horseradish peroxidase as antigen.		1976	798738
possible determinants of the degree of retrograde neuronal labeling with horseradish peroxidase.		1975	803395
the efferent connections of the suprachiasmatic nucleus of the hypothalamus.	the efferent connections of the suprachiasmatic nucleus of the hypothalamus have been studied in the rat by the injection of 3h-proline into the nucleus and the surrounding regions of the rostral hypothalamus, and by the injection of the enzyme marker, horseradish peroxidase, into the region of the ventromedial hypothalamic nucleus. after an injection of 3h-proline confined to the ventral portion of the suprachiasmatic nucleus, transported label can be followed, in the autoradiographs, dorsally ...	1975	803516
ocular vascular and epithelial barriers to microperoxidase.	microperoxidase (mp) is an ultrastructural tracer of small molecular weight (1,900) derived from horse heart cytochrome c. within the central nervous system, it is capable of entering the periaxonal space which is not open to horseradish peroxidase (hpr). because of its small size and unique behavior, mp was used to probe ocular vascular and epithelial barriers. mp did not enter any ocular space from which hrp was excluded. this tracer established a new lower level of permeability in these ocula ...	1975	806549
immunochemical studies of the endocrine cells of the gastrointestinal tract. i. the use and value of peroxidase-conjugated antibody techniques for the localization of gastrin-containing cells in the human pyloric antrum.	this paper describes and immunochemical localization of gastrin-containing endocrine cells in the human pyloric antrum using antibodies conjugated to horseradish peroxidase. with pre-fixed cryostat sections, a distinct clear-cut staining of gastrin-containing cells can be obtained either by direct or indirect single stain procedures, but may cells containing endogenous peroxidase activity also stain. in order to abolish staining due to endogenous peroxidase, sections were pretreated with a numbe ...	1975	807542
an efferent connection of the solitary cells of meynert. a study with horseradish peroxidase in the marmoset callithrix.		1975	809097
certain connections of the visual cortex of the monkey shown by the use of horseradish peroxidase.		1975	809098
an electron microscopic study of retrograde and orthograde transport of horseradish peroxidase to the lateral geniculate nucleus of the monkey.		1975	809099
confirmation of the location of spinothalamic neurons in the cat and monkey by the retrograde transport of horseradish peroxidase.		1975	809119
amygdaloid projections to prefrontal granular cortex in rhesus monkey demonstrated with horseradish peroxidase.		1975	810217
histochemical characterization of a neocortical projection of the nucleus locus coeruleus in the squirrel monkey.	histochemical evidence is presented for a catecholamine-containing projection from the nucleus locus coeruleus to the neocortex in the squirrel monkey. the innervation of superior temporal gyrus has been examined in particular. glyoxylic acid-induced fluorescence shows an extensive arborization of fine, catecholamine-containing fibers with prominent varicosities in all layers of the neocortex. the nucleus locus coeruleus is identified as a source of these fibers by both ortho- and retrograde axo ...	1975	810499
monkey retinal ganglion cells: morphometric analysis and tracing of axonal projections, with a consideration of the peroxidase technique.	this paper presents evidence on the retinal distribution and central projections of retinal ganglion cells of various cell body sizes in the adult macaque monkey. the ganglion cell sizes have been determined by computer assisted measurement of camera lucida drawings at various eccentricities of both flat mounted and sectioned retinae. the pattern of projections of individual ganglion cells to the dorsal lateral geniculate nucleus and superior colliculus has been studied using retrograde axonal t ...	1975	810500
the origin of efferent pathways from the primary visual cortex, area 17, of the macaque monkey as shown by retrograde transport of horseradish peroxidase.	the retrograde transport of horseradish peroxidase has been used to identify efferent cells in area 17 of the macaque. cells projecting to the lateral geniculate nucleus are small to medium sized pyramidal neurons with somata in lamina 6 and the adjacent white matter. the projection to the parvocellular division arises preferentially from the upper half of lamina 6, while that to the magnocellular division arises preferentially from the lower part of the lamina. the projection to both superior c ...	1975	810501
use of horseradish peroxidase-labelled antiglobulin for the colorimetric quantitation of erythrocyte antibodies.	horseradish peroxidase (hrp)-labelled antiglobulin has been used extensively as a histochemical marker. in the method described the stable reaction product formed by using 3,3'-diaminobenzidine (dab) as the hydrogen donor in the peroxidase reaction was dissolved in a fluoro alcohol, 1,1,1,3,3,3-hexafluoroisopropanol (hpf). reproducible standardisation curves resulted with hrp. based on these observations a colorimetric assay procedure was developed to quantitate the amount of antibody coating re ...	1975	811709
attempts at differentiation of the structure of neurons in the corpus geniculatum laterale of the pig by the immunocytochemical technique.	the investigations were carried out on the corpus geniculatum laterale of the pig with the aim to differentiate the structure of its neurons on the basis of the antigenic properties of the nervous tissue. the antigenic material consisted of the homogenate of the corpus geniculatum laterale (h) and of its nuclear (p1) and mitochondrial (p2) fractions. the antigens were used to immunize 3 groups of rabbits, the 4th one serving as control. the antigens were prepared according to requirements of the ...	1975	812788
blood-aqueous barrier can be circumvented by lowering intraocular pressure.	rhesus monkeys were injected intravenously with hypertonic urea (9 ml/kg body weight of 30% urea in 10% invert sugar) and the intraocular pressure was measured with an applamatic tonometer. when this pressure reached its minimum (20% of the normal value) horseradish peroxidase (molecular weight 40,000; radius of an equivalent hydrodynamic sphere about 2.5 nm; 0.5 g/kg body weight), was injected intravenously. twenty minutes following peroxidase administration, either aqueous humor was sampled fr ...	1976	813231
[demonstration of direct projections between the ponto-mesencephalic tegmentum and the visual cortex of the cat using a peroxidase technique].	using a retrograde tracer technique with horseradish peroxidase, we have identified the afferent connections to the visual cortex from the dorsolateral pontine tegmentum (locus coeruleus, nucleus parabrachialis medialis and nucleus parabrachialis lateralis) as well as from the dorsal raphe nucleus.	1975	813889
structural alterations in the ciliary process and the blood-aqueous barrier of the monkey after systemic urea injections.	the structural alterations induced in the monkey ciliary epithelium by intravascular injection of urea solutions were studied with the light and electron microscopes. arterial injection of urea resulted in destruction of the nonpigmented epithelium and the consequent breakdown of the blood-aqueous barrier. the intravenous injection did not significantly affect the structural integrity of the ciliary epithelium. the intercellular zonulae occludens were not altered and the intercellular pathway fr ...	1976	814818
deoxyribonucleic acid replication in single cells and chromosomes by immunologic techniques.	antibodies to 5-bromodeoxyuridine (brdu) or iododeoxyuridine may be used to identify cells or regions of chromosomes in which de novo deoxyribonucleic acid synthesis has occurred. the antibodies to brdu were produced in rabbits by injection of the antigen, a conjugate between bovine serum albumin and bromouridine (bru), or iodouridine. specific antibodies were produced by affinity chromatography on ah-sepharose 4b to which had been coupled bru. anti-bru cross-reacts with iodeodeoxyuridine. indir ...	1976	815428
midbrain, diencephalic and cortical relationships of the basal nucleus of meynert and associated structures in primates.	the structure and connectivity of the basal nucleus of meynert, the substantia innominata in which it lies, and certain related areas have been examined in new world and old world monkeys, using retrograde and anterograde axonal transport methods. experiments using the retrograde, horseradish peroxidase method confirm the observations of kievet and kuypers ('75) that the basal nucleus and substantia innominata project directly, heavily and with a somewhat crude topography upon the neocortex. exp ...	1976	818134
immunofluorescence and immunoperoxidase observations of anti-lactic dehydrogenase-1 antibody.	for immunoelectron-microscopic demonstration of lactic dehydrogenase (ldh), anti-subunit-b antibody was produced in rabbits by immunization with purified pig ldh-1 (b4) isoenzyme. immunoglobin fragment fab' was obtained from anti-ldh serum immunoglobulin g and conjugated with horseradish peroxidase. subunit-b-containing ldh was present diffusely in the sarcoplasm of the pig heart muscle. there was concentration of ldh in the 1-band. ldh reaction was also present in the mitochondrial intermembran ...	1976	818310
projections from the dorsal lateral geniculate nucleus to prestriate cortex in the squirrel monkey as demonstrated by retrograde transport of horseradish peroxidase.		1976	819105
areal and laminar distribution of some pulvinar cortical efferents in rhesus monkey.	the areal and laminar distribution of the cortical efferents of the medial, lateral and inferior pulvinar nuclei (pm, pl and pi respectively) were determined in rhesus monkey using autoradiography and horseradish peroxidase (hrp). the autoradiographic data indicated that: areas 8a, 45 and 46 on the convexity and 11 and 12 on the orbital surface of the frontal lobe received projections from pm; areas 20, 21 and 22 in temporal lobe received projections from pm primarily with caudal-medial parts of ...	1976	823181
spinal projections from the nucleus locus coeruleus and nucleus subcoeruleus in the cat and monkey as demonstrated by the retrograde transport of horseradish peroxidase.	the rostral pons of the cat and rhesus monkey were examined for the presence of labeled cells following injections of horseradish peroxidase (hrp) into the lumbar spinal cord. labeled cells were found in the ipsilateral dorsolateral pontine tegmentum and in the contralateral ventrolateral pontine reticular formation. in both the cat and monkey, labeled cells were located in the nucleus locus coeruleus, nucleus subcoeruleus, in or near the kölliker-fuse nucleus, and in the ventral part of the lat ...	1976	824030
a horseradish peroxidase method for the identification of the efferents of acetyl cholinesterase-containing neurons.		1976	826585
the effect of alloxan on the permeability of isolated pancreatic islets to horseradish peroxidase.	the effect of alloxan on the permeability of isolated pancreatic islets to horseradish peroxidase was studied by a perifusion system, which allowed simultaneous monitoring of glucose-induced insulin secretion. rat islets were perifused with a 5 mg/ml glucose solution containing horseradish peroxidase for ten or thirty minutes, with or without prior exposure to alloxan (20 mg%) for five minutes. control islets without alloxan treatment showed few necrotic beta cells diffusely infiltrated with exo ...	1976	827103
the mammalian rete testis--a morphological examination.	the fine structure of the rete testis was examined in several primates, domestic animals and rodents. the rete testis consists of a series of interconnected wide channels lined with a simple cuboidal to columnar epithelium, resting on a thick basal lamina. beneath the basal lamina dense bundles of collagen fibrils and a few blood vessels, lymphatics or nerve tissue are found. the epithelial cells are characterized by large, deeply indented nuclei, spherical or short rod-shaped mitochondria, supr ...	1976	827216
[problems posed by the interpretation of peroxidase labelling of various neurons in cyprinidae].	the origin of a centrifugal visual pathway in cyprinids could not be demonstrated with the technique involving the labelling of cell bodies by retrograde transport of horseradish peroxidase. the hypothalamic labelling following intraocular injection of hrp is localized in neurosecretory structures which take up the enzyme that has passed into the circulatory system. identical results were obtained following direct intracardiac injection. thus extreme caution must be taken in attempting to interp ...	1976	827368
experimental retinal branch vein occlusion in the monkey. histopathological and ultrastructural studies.	light and electron microscopical studies of monkey retinae after experimental branch vein occlusion by argon laser reveal that the immediate reaction in the corresponding region is one of capillary engorgement and dilatation followed by oedema (intra- and extracellular), and plasma leakage as shown by intravenous injection of horseradish peroxidase. vascular stasis is characterized by packing of erythrocytes in the capillary lumen, early thrombus formation, and focal haemorrhages. progressive de ...	1976	827828
is there a blood-brain barrier at the optic nerve head?	the retina and optic nerve have been demonstrated to possess a blood-brain barrier that prevents the passive passage of protein and certain dyes from the blood vessels into the extracellular space. our observations suggest that an exception is present at the normal optic disc. using horseradish peroxidase as a tracer for electron microscopy and the normal rhesus monkey as the experimental animal, we have demonstrated that in certain regions of the optic nerve head, horseradish peroxidase from th ...	1975	828849
afferent projections to the cat locus coeruleus as visualized by the horseradish peroxidase technique.		1977	830382
serotonergic afferents to the dorsal raphe nucleus: evdience from hrp and synaptosomal uptake studies.	afferent connections of the serotonin (5-ht)-containing dorsal raphe nucleus were investigated in the rat utilizing the horseradish peroxidase (hrp) retrograde cell labeling technique. small quantities (0.1-0.5 mul) of hrp solutions were infused into the dorsal raphe, and the brains were examined 19-72 h later for retrograde transport of the enzyme. intrinsic connections within the dorsal raphe nucleus were revealed by this mapping technique, as was an input to the dorsal raphe from another sero ...	1977	830388
pseudopodia formation by neurosecretory granules.	ultrastrucal studies of the mouse neurohypophysis, under various experimental conditions, revealed a number of neurosecretory granules (nsg) bearing single pseudopodia-like protrusions. some nsg adhered to the axolemma via pseudopodia; other nsg, distant from the axolemma, budded electron lucent microvesicles from the tip of the pseudopod. pseudopodia counts were made on electron micrographs, and calculated as a percentage of the nsg population. in neural lobes from intact mice, small numbers of ...	1977	830428
amygdala afferents from the mediobasal hypothalamus: an electrophysiological and neuroanatomical study in the rat.	electrophysiological techniques and the retrograde transport of horseradish peroxidase (hrp) were used to determine the efferent projections from the caudal mediobasal hypothalamus to the amygdala. in pentobarbital anesthetized rats, the activity of 1780 mediobasal hypothalamic neurons was examined for response to stimulation sites in the amygdala and stria terminalis. evidence of orthodromic activation from both stimulation sties was commonly observed. sixty-five cells mostly located in the ven ...	1977	832160
protamine induced intracellular uptake of horseradish peroxidase and vacuolation in mouse skeletal muscle in vitro.	the uptake in vitro of horseradish peroxidase (hrp) in mouse skeletal muscle was examined by electron microscopy and chemical determination. in muscles exposed to an hrp solution for 60 min at +37 degrees c, hrp infiltrated the basal lamina of muscle fibres and caused an intense labelling of their sarcolemma. in addition hrp was found within the transverse tubules. exposure to hrp for 30 min at +37 degrees c followed by hrp together with a polycationic protein (protamine) for 30 min at +37 degre ...	1977	832308
effect of pentobarbital on uptake of horseradish peroxidase by rabbit cortical synapses.		1977	832696
sequential uptake of horseradish peroxidase by lymphoid follicle epithelium of peyer's patches in the normal unobstructed mouse intestine: an ultrastructural study.	membranous epithelial or "m" cells in lymphoid follicle epithelium of peyer's patches extend between the adjacent columnar cells, forming a membrane separating lymphocytes in the epithelial cell layer from the intestinal lumen. they lack developed microvilli, glycocalyx, or terminal web but contain numerous vesicles. transport of particles from ligated intestinal loops by such cells without evidence of further uptake of these particles by lymphocytes has been described by others. horseradish per ...	1977	832793
pulmonary capillary permeability to hrp in dogs: a physiological and morphological study.	pulmonary capillary permeability was studied in 10 normal anesthetized dogs using horseradish peroxidase (hrp) as tracer. physiological studies involved measurement of left ventricular, pulmonary arterial, and wedge pressures, and of right lymphatic duct (rld) and thoracic duct lymph flows. plasma and interstitial oncotic pressures were calculated from protein measurements. after 1 h of lymph collection, hrp was injected intravenously at three total dose levels, low (20-30 mg/kg), medium (55 mg/ ...	1977	833071
immunoelectronmicroscopic localization of alpha-actinin in skeletal muscle cells.	the ultrastructural distribution of alpha-actinin in skeletal myocytes was studied by a sensitive method that permits localization of antigenic proteins in intact fixed cells rather than in glycerol estracted tissues or isolated cell fractions. binding to the z line of specific antiserum produced in rabbits immunized with highly purified alpha-actinin was demonstrated by deposition at this site of a reaction product of horseradish peroxidase that had been coupled to antigen-binding fragments of ...	1977	833423
concanavalin a receptors on the surfaces of human breast cancer cells in organ culture.	the binding of concanavalin a (con a) at the free apical membranes of surface tumor cells in human breast cancer explants grown in organ culture was studied cytochemically with horseradish peroxidase (hrp) as a marker. on the cell membranes in aldehyde-fixed explants or explants exposed to con a at 4 degrees c, a continuous label covered the entire free surface of the cell, which indicated the dispersed distribution of con a binding sites. the binding of con a at 20 degrees c resulted in discont ...	1977	833861
amygdaloid projections to prefrontal and motor cortex.	direct projections from the amygdala to the entire frontal cortex were demonstrated in the cat using the retrograde transport of horseradish peroxidase. injections throughout the prefrontal cortex labeled neurons in the ipsilateral basal magnocellular amygdaloid nucleus; injections in the premotor and motor cortices labeled neurons in the same nucleus plus a few cells in the anterior amygdaloid area.	1977	836591
evidence of the dual innervation of the cat stomach by the vagal dorsal motor and medial solitary nuclei as demonstrated by the horseradish peroxidase method.		1977	837215
electrophysiological and horseradish peroxidase studies of precerebellar afferents to the nucleus interpositus anterior. i. climbing fiber system.	the input to the nucleus interpositus anterior (nia) of the cat from the inferior olive (io) was studied by stimulating the io while recording intracellularly from the nia, and by the retrograde transport of horseradish peroxidase (hrp). stimulation of the io evoked monosynaptic epsps in nia neurons. the cells labeled in the io following electrophoretic and pressure injection of hrp into nia were located in the rostral parts of the dorsal and medial accessory olive. stimulation of the io also po ...	1977	837228
electrophysiological and horseradish peroxidase studies of precerebellar afferents to the nucleus interpositus anterior. ii. mossy fiber system.	inputs to the nucleus interpositus anterior (nia) of the cat from precerebellar nuclei which are thought to give rise to mossy fibers were studied using electrophysiological and anatomical techniques. stimulation of one of these precerebellar nuclei, the lateral reticular nucleus (lrn) evoked monosynaptic epsps in nia neurons. these epsps were followed by polysynaptic ipsps and late depolarization mediated by the response of the cerebellar cortex. similar responses were occasionally seen followi ...	1977	837229
habenular and other midbrain raphe afferents demonstrated by a modified retrograde tracing technique.	afferents to th midbrain dorsal and median raphe nuclei in the rat were studied by means of the horseradish peroxidase (hrp) retrograde transport method. the hrp was given by means of a modified iontophoretic delivery technique. this technique permitted an efficient and localized deposition of a high concentration of hrp into the raphe nuclei. afferents to the raphe as determined by this method could be categorized into 2 classes; those exclusively to the raphe and those also positive for adjace ...	1977	837230
central connections of ventral root afferents as demonstrated by the hrp method.	to study the central connections of ventral root afferents, horseradish peroxidase was injected into the lumbosacral spinal cord of the cat and the appropriate dorsal root ganglia were examined from segments with (1) both dorsal and ventral roots intact, (2) both roots sectioned, and (3) only the dorsal root sectioned. the key finding was that a number of labeled cells (up to 133) were observed in the ganglion after dorsal rhizotomy. we interpret these findings to mean that the central processes ...	1977	838891
altered renal cortical reabsorption of protein and urinary excretion of sodium in relation to vascular leakage induced by horseradish peroxidase.	the intravenous injection of horseradish peroxidase (hrp) into rats of the sprague-dawley strain caused vascular leakage as detectable by a 20-40% increase in the hematocrit and a 15-20% decrease in plasma protein concentration. these changes did not occur when the same amounts of hrp were injected into rats pretreated with antagonists to histamine and serotonin. after pretreatment with the antagonists, the reabsorption of hrp by the proximal tubule cells (the concentration of hrp in the total p ...	1977	839063
the distribution of peroxidases in the sciatic nerves of normal and hexachlorophene intoxicated developing rats.	horseradish peroxidase (mol. wt. 40 000) or microperoxidase (mol. wt. 1900) were injected over the sciatic nerve of normal or hexachlorophene (hcp) intoxicated developing rats (3,7,14 and 21 days). light and electron microscopic studies of nerves after histochemical staining for peroxidase revealed: a) the perineurial barrier to the two peroxidases was established in 21 day normal and hcp intoxicated rats; b) in animals 3-14 days, the perineurial barrier to both peroxidases was not formed and pe ...	1977	839249
cerebral cortical changes in acute experimental hypertension: an ultrastructural study.	multiple focal cortical areas of increased vascular permeability to tracer substances occur in experimental hypertensive encephalopathy. in this study, rats with angiotensin-induced acute hypertension were used to determine whether increased permeability was associated with focal cerebral edema and if so, the tissue component involved. in addition, the mechanism of increased permeability and the types of vessels involved were investigated using horseradish peroxidase as a tracer. quantitative mo ...	1977	839731
experimental immunologic thrombocytopenia in dogs: a study of thrombocytopenia and megakaryocytopoiesis.	immunologic thrombocytopenia was induced in dogs by intravenous or intraperitoneal injection of rabbit anti-canine platelet serum (aps) or by intraperitoneal injection of the igg fraction of the aps. in contrast, dogs injected with normal rabbit serum (nrs) or the igm fraction of the aps did not develop thrombocytopenia. marrow samples obtained at 24-96 h after inoculation of the igg fraction contained megakaryocytes giving a positive reaction with flurescein isothiocyanate or horseradish perox ...	1977	841193
cellular uptake of exogenous horseradish peroxidase in mouse peripheral nerve.	horseradish peroxidase (hrp) was given to adult normal mice either intravenously or locally around the sciatic nerve. after varying time intervals the animals were sacrificed and fixed by whole body perfusion. the sciatic nerve was sampled and the distribution of peroxidatic activity was studied by light and electron microscopy. after both types of hrp administration the tracer was rapidly taken up by epi- and endoneurial cells with the ultrastructural features of macrophages. when increasing do ...	1977	842294
stability of the intra-epithelial component of the blood-testis barrier in epinephrine-induced testicular degeneration in syrian hamsters.	adult male syrian hamsters were given daily intraperitoneal injections of epinephrine (1.0 mg/kg) and papaverine, a vasodilator, (60 mg/kg) for a period of ten days. after the treatment period, lanthanum and horseradish peroxidase tracer studies were used to examine the intra-epithelial component of the blood-testis barrier. degenerating tubules often exhibited only sertoli cells and spermatogonia, or sertoli cells alone. sertoli cell processes in the degenerating tubules often arched out from t ...	1977	842474
the pontine projection to the cerebellar vermal visual area studied by means of the retrograde axonal transport of horseradish peroxidase.	following injections of horseradish peroxidase (hrp) in cerbellar vermal lobules vi, viia and b, viia and b in the cat, the distribution of labeled cells in the pontine nuclei was mapped in drawings of serial transverse and horizontal sections. the labeled pontine cells are distributed in 4 largely longitudinal columns, situated in the dorsolateral, peduncular, lateral and paramedian pontine nucleus (referred to as columns a, b, c and d, respectively). the majority of afferents to the vermal, vi ...	1977	843923
vestibular and medullary brain stem afferents to the abducens nucleus in the cat.	brain stem neurons that project to the abducens nucleus (nvi) were labeled by the technique of retrograde transport of horseradish peroxidase (hrp). following iontophoresis of hrp into nvi a large number of labeled cells are found in the ipsilateral vestibular nuclear complex, extending from the rostral medial vestibular nucleus into the ventral lateral vestibular nucleus. a smaller number of hrp-positive cells are also found in the contralateral medial vestibular nucleus. in addition, labeled c ...	1977	843924
vesicular transport of horseradish peroxidase from brain to blood in segments of the cerebral microvasculature in adult mice.	the transfer of protein from the cerebral ventricles to the parenchymal bloodstream in mice was studied by electron microscopy. after perfusion with the protein tracer horseradish peroxidase (hrp; m.w. approx. 40,000) through the cerebral ventricles, the tracer penetrated the ependymal lining of the ventricles and was found in the extracellular space of the neuropil close to the ependyma. hrp was also seen in the vascular basement membrane and in endothelial vesicles opening at the abluminal end ...	1977	843935
the uptake of horseradish peroxidase by cortical synapses in rat brain. an in vivo study.	horseradish peroxidase (hrp) was introduced directly into the cerebral cortex of adult rats, which were allowed to survive for 60 min before perfusion fixation. after the tissue had been incubated to demonstrate hrp at the lm and em levels, blocks of cortical tissue were taken at varying distances from the injection site. these eight blocks of tissue constituted a time sequence for hrp diffusion. qualitative examination of the presynaptic terminals showed that the most commonly encountered profi ...	1977	844083
afferent connections of the habenular nuclei in the rat. a horseradish peroxidase study, with a note on the fiber-of-passage problem.	the afferent connections of the habenular complex in the rat were examined by injecting horseradish peroxidase (hrp) into discrete portions of the habenular nuclei by microelectrophoresis. 1. hrp deposits confined to the lateral half of the lateral habenular nucleus labeled a multitude of cells in the entopeduncular nucleus. numerous labeled cells also appeared in such cases in the lateral hypothalamus, indicating that the lateral habenular nucleus is a major convergence point of projections fro ...	1977	845280
the commissural projection of the superior colliculus in the cat.	the origin, course, and termination of the commissural projection of the superior colliculus were studied using the orthograde and autoradiographic tracing method and the retrograde method utilizing horseradish peroxidase. the complementary and mutually confirming sets of data showed that the commissural fibers interconnect a restricted region of the colliculi. this region includes the strata grisea intermedium and profundum and to a lesser degree the stratum opticum. it extends throughout only ...	1977	845285
increased vesicular transfer of horseradish peroxidase across cerebral endothelium, evoked by acute hypertension.	acute hypertension in rats was produced by intravenous infusion of metaraminol bitartrate (aramine). the permeability to intravenously injected horseradish peroxidase (hrp) was increased across the cerebral arterioles, capillaries and venules. from the basement membranes of the vessel walls the protein tracer moved into the extracellular spaces of the adjacent neuropil. no endothelial cell damage was observed. the tight junctions between endothelial cells were intact and prevented intercellular ...	1977	848278
amino-acid sequences of heme-linked, histidine-containing peptides of five peroxidases from horseradish and turnip.	in a previous paper we have characterized five plant peroxidases, p1, p2, p3 and p7 of turnip and horseradish isoperoxidase c by peptide mapping studies, and only found two highly homologous sequences present in all. both contained histidine. the findings supported previous suggestions of two histidine sequences nearthe peroxidase heme prostetic group. in the present paper we present the amino acid sequences around the histidine residues of all four turnip peroxidases, i. e. of 25 residues aroun ...	1977	849740
attachment and ingestion of mycoplasmas by mouse macrophages. ii. scanning electron microscopic observations.	mycoplasmas adhere closely to the central region of the surface of mouse peritoneal macrophages in vitro. they do not appear connected to each other or the macrophage membrane, and they induce no change in the surface of the cell. after addition of antimycoplasma antibody, mycoplasmas show interconnections and the cell shows an increase occurrence of ruffled membrane and folding over the mycoplasmas. large and small lacunae appear in the membrane at sites other than those taking in organisms, an ...	1977	851171
thalamic projections to parietal cortex.	thalamic projections to parietal regions of cerebral cortex were investigated in the cat by retrograde degeneration and retrograde transport techniques. studies of retrograde cellular changes indicate cortically projecting cells in the lateroporterior nucleus (lp), rostral pulvinar (pul), and the ventral part of the laterodorsal nucleus (ld). when lesions were placed in the cortex of young kittens, clear chromatolytic changes and cell loss were also consistently observed in middle and caudal par ...	1977	851845
convergence of excitatory synaptic inputs to caudate spiny neurons.	intracellular recordings were obtained from cat caudate nucleus neurons. these cells responded with convergent, monosynaptic epsps to cerebral cortex, intralaminar thalamus, and substantia nigra stimulation. intracellular injection of horseradish peroxidase into these electrophysiologically defined neurons shows that they represent the spiny neuron of the caudate based upon somadendritic morphology. the axons of these cells are appreciably longer than previous reports indicate. several lines of ...	1977	851861
permeability and patency of retinal blood vessels in experimental diabetes.	increased permeability of retinal blood vessels in human diabetic retinopathy is well known clinically. its morphologic equivalent is unknown. in dogs with 5 years of poorly controlled alloxan diabetes and nonproliferative diabetic retinopathy comparable to that of man, permeability and patency of retinal blood vessels were tested with the protein tracer horseradish peroxidase and evaluated by electron microscopy. a breakdown of the blood-retinal barrier was found associated with extensive trace ...	1977	852945
active site models of horseradish peroxidase compound i and a cytochrome p-450 analogue: electronic structure and electric field gradients.		1977	853195
internalization of lectins in neuronal gerl.	conjugates of ricin agglutinin and phytohemagglutinin with horseradish peroxidase (hrp) were used for a cytochemical study of internalization of their plasma membrane "receptors" in cultured isolated mouse dorsal root ganglion neurons. labeling of cells with lectin-hrp was done at 4 degrees c, and internalization was performed at 37 degrees c in a culture medium free of lectin-hrp. 15-20 min after incubation at 37 degrees c, lectin-hrp receptor complexes were seen in vesicles or tubules located ...	1977	856827
the organization of the pulvinar in the grey squirrel (sciurus carolinensis). i. cytoarchitecture and connections.	the posterior neocortex in the grey squirrel, sciurus carolinensis, includes an extensive region which receives projections from the pulvinar. previous studies have demonstrated that this cortical region can be subdivided on the basis of differences in cytoarchitecture and electrophysiologically defined representations of the visual field. the main purpose of the present paper was to determine whether these cortical subdivisions could be related to corresponding subdivisions in the pulvinar. the ...	1977	856889
anatomy of interhemispheric connections in the visual system of boston siamese and ordinary cats.	in siamese cats, previous studies have shown that a genetic mutation causes retinogeniculate fibers in each eye which arise from the temporal retina representing the 20 degrees of ipsilateral visual field adjacent to the vertical meridian to cross aberrantly in the optic chiasm, thereby terminating in the wrong lateral geniculate nucleus. the abnormality is expressed subsequently at the level of the visual cortex. this paper presents anatomical evidence that the pattern of commissural visual con ...	1977	856894
the projection from the lateral geniculate nucleus onto the visual cortex in the cat. a quantitative study with horseradish-peroxidase.	horseradish-peroxidase (hrp) was injected (9-18 microng in 0.03-0.06 micronl) into cortical areas 17, 18 or 19 of 11 adult cats. after survival times of 17 hours to 7 days, the thalamus was examined for retrogradely hrp labelled nerve cells in serial transverse sections. from these sections, the percentage of labelled cells occurring in each subdivision of the dorsal lateral geniculate nucleus (lgnd) was calculated for each animal. one case each for injections in areas 17, 18, and 19 was then ch ...	1977	856895
site of horseadish peroxidase transport by the small intestine of the neonatal rat.	the absorption of horseradish peroxidase was estimated in 10-day-old rats by measuring plasma peroxidase levels following intraduodenal admininstration. peak levels occurred 45 min after injection. plasma peroxidase levels, 45 min after injection into five ligated segments along the small intestine, were significantly lower in rats with distal segments than in rats with proximal segments. this indicates that the proximal small intestine, including the duodenum, may play a significant role in non ...	1977	861316
origins of primary afferent fibers in the spinal ventral roots in the cat as demonstrated by the horseradish peroxidase method.		1977	861724
ultrastructural characteristics of the brain and blood-brain barrier in experimental seizures.	during experimental seizures, the blood-brain barrier (bbb) is broken; tracer substances such as i131-albumin, evans blue and horseradish peroxidase (hrp) geographically locate the barrier breakdown primarily in the diencephalon. using rats, we have induced seizures with electroshocks and demonstrated the breakdown of the bbb with evans blue and hrp. we have shown that (1) the bbb breakdown is proportional to the number of electroconvulsant shocks (es) given; (2) the mechanism of increased barri ...	1977	861758
control of density and microredistribution of concanavalin-a receptors in rat thymocytes at 4 degrees c.	in a previous paper, we reported that at 4 degrees c, a cooperative binding of concanavalin a to rat thymocytes is observed which corresponds to a modification of the membrane leading to the recruitment of receptors and their immobilization. in this paper, we report that both phenomena are modulated at 4 degrees c by drugs such as colchicine and cytochalasin b; cooperative binding of concanavalin a, which reflects receptor recruitment is only slightly modified by each drug alone. when the two dr ...	1977	862619
polymerisation of oestradiol by potassium ferricy anide and by horseradish peroxidase.		1977	862904
wafer embedding: specimen selction in electron microscopic cytochemistry with osmiophilic polymers.	a new wafer embedding procedure is described that permits light microscopic screening of embedded tissue prior to ultrathin sectioning. it is particularly valuable when used on specimens obtained with an automatic sectioner and treated cytochemically to obtain visible intermediate or visible and electron opaque final reaction products. aldehyde-fixed tissues are cut into sections with an automatic sectioner, incubated cytochemically osmication if required, then embedded in epoxy resin between fl ...	1977	863747
automated fluorometric analysis of galactose in blood.	in galactosemia, prevention of mental retardation depends on early recognition of the disorder and institution of dietary restriction of galactose. we describe an automated fluorometric micromethod for galactose in whole blood spotted on filter paper. galactose is oxidized by galactose oxidase to d-galacto-hexadialdose and h2o2 and measured as the highly fluorescent condensation product of homovanillic acid formed when h2o2 is acted upon by horseradish peroxidase. the procedure is 10-fold more s ...	1977	870260
experimental studies on cardiac muscle cell adaptation to insult.	studies were carried out in rats on myocardial adaptation to injury produced by left coronary artery ligation. in the ischemic zone, open collaterals were present as shown by studies using the fine structural extracellular protein tracer, horseradish peroxidase (hrp). this phenomenon may explain the inhomogeneous cardiac muscle cell alteration in the early phase of coronarogenic myocardial injury. reperfusion, as evidenced by the influx of hrp into the damaged cells, unmasked sarcolemmal membran ...	1977	871298
reticulo-cuneate projections as revealed by horseradish peroxidase axonal transport.		1977	871918
effect of mixed solvents on the formation of horseradish peroxidase compound i. the importance of diffusion-controlled reactions.	in aqueous glycerol solutions, the rate constant for the formation of compound i of horseradish peroxidase from hydrogen peroxide is independent of the viscosity. however, whem m-chloroperbenzoic acid is used to form compound i, the rate is viscosity dependent, indicating that this reaction is diffusion controlled. the problems in obtaining accurate information about diffusion-controlled reactions are discussed as is the possible significance of diffusion control. ethanol has been used in previo ...	1977	880290
descending connections between superior olivary and cochlear nuclear complexes in the cat studied by autoradiographic and horseradish peroxidase methods.	the descending pathways from the superior olivary complex (so) to the cochlear nuclear complex (cn) were investigated in 58 cats using labelled aminoacid and horseradish peroxidase transport techniques. descending connections were found coursing bilaterally through the trapezoid body (tb) and ipsilaterally in the intermediate and dorsal acoustic striae. the dorsolateral periolivary nucleus (dlpo) sends fibres through both the intermediate and dorsal striae, which are joined by others from the la ...	1977	880993
direct projections from thalamic intralaminar nuclei to extra-striate visual cortex in the cat traced with horseradish peroxidase.	thalamic projections to the visual cortex were investigated using the horseradish peroxidase tracing technique. besides confirmation of a distinct origin of thalamic projections to striate and extra-striate visual cortex, afferents of the intralaminar nuclei (iln) to visual cortex were demonstrated. these projections of iln were shown to be specific in that they terminate in areas 18, 19 and clare bishop but not area 17. the coupling of these intralaminar projections on to the extra-striate visu ...	1977	880998
ultrastructural observations of the abducens nucleus of the cat after injection of horseradish peroxydase into the lateral rectus muscle.	the ultrastructure of cat abducens motoneurones is described, after labelling by retrograde axonal transport of horseradish peroxydase. the motoneurones were densely covered with synaptic boutons of both the "terminal" and "en passant" types and axo-axonal synapses were observed on the initial segment of motoneurone axons. within the population of axons forming the vith nerve, multiple branching was encountered and axons closely apposed at the nodes of ranvier were seen.	1977	881006
evaluation of the horseradish peroxidase-scopoletin method for the measurement of hydrogen peroxide formation in biological systems.		1977	883627
anatomical and physiological characterization of avian sympathetic cardiac efferents.	in the preceding paper6 describing the right sympathetic cardiac nerve of the pigeon we reported a fast compound action potential component with no chronotropic effect, and single unit recordings from sympathetic postganglionic neurons demonstrated that this action potential component was not reflected in the antidromic postganglionic latencies. electron microscopy then indicated the presence of numerous myelinated fibers in the nerve, and together these findings suggested the existence of a sub ...	1977	884549