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invasive potential of bacterial isolates associated with subclinical bovine mastitis.this work describes differences in the invasive ability of bacterial isolates associated with mastitis. invasion ability was determined by the uptake and survival in a primary culture of bovine mammary epithelial cells (bmec). bmec were isolated from a healthy lactating cow and characterized by their morphology, immunostaining for cytokeratin and the detection of beta- and kappa-casein mrnas. ten bacterial isolates comprising the staphylococcal species staphylococcus aureus (3), staphylococcus e ...200616624358
molecular identification and differentiation of brevibacterium species and strains.amplified ribosomal dna restriction enzyme analysis (ardra), pulsed field gel electrophoresis (pfge) and ribotyping were used to differentiate among 24 strains of brevibacterium linens, brevibacterium casei and brevibacterium epidermidis obtained from type culture collections or isolated from various smear ripened cheeses. ardra was applied to the 16s rdna. b. linens was shown to be a quite heterogenic group with 2 to at least 4 copies of rrn operons per strain with aberrant nucleotide sequences ...200716624515
application of streptomyces and brevibacterium cholesterol oxidase for total serum cholesterol assay by the enzymatic kinetic method.using non-esterified cholesterol standard, brevibacterium and streptomyces are found as suitable sources of cholesterol oxidase for kinetic cholesterol assay. for clinical use, we investigated the suitability of these enzymes for cholesterol determination in human serum.200616678143
central venous catheter infection with brevibacterium sp. in an immunocompetent woman: case report and review of the literature.brevibacterium spp. were considered apathogenic until a few reports of infections in immunocompromised patients were published. herein, we present a case of a catheter-related septicemia with brevibacterium casei in an immunocompetent patient receiving continuous iloprost infusion for pulmonary arterial hypertension and review the clinical presentation of this mainly emerging opportunistic pathogen.200616703303
purification and characterization of fumarase from corynebacterium glutamicum.fumarase (ec 4.2.1.2) from corynebacterium glutamicum (brevibacterium flavum) atcc 14067 was purified to homogeneity. its amino-terminal sequence (residues 1 to 30) corresponded to the sequence (residues 6 to 35) of the deduced product of the fumarase gene of c. glutamicum (genbank accession no. bab98403). the molecular mass of the native enzyme was 200 kda. the protein was a homotetramer, with a 50-kda subunit molecular mass. the homotetrameric and stable properties indicated that the enzyme be ...200616717409
characterisation of prototype nurmi cultures using culture-based microbiological techniques and pcr-dgge.undefined nurmi-type cultures (ntcs) have been used successfully to prevent salmonella colonisation in poultry for decades. such cultures are derived from the caecal contents of specific-pathogen-free birds and are administered via drinking water or spray application onto eggs in the hatchery. these cultures consist of many non-culturable and obligately anaerobic bacteria. due to their undefined nature it is difficult to obtain approval from regulatory agencies to use these preparations as direc ...200616814892
lateral transfers of serine hydroxymethyltransferase (glya) and udp-n-acetylglucosamine enolpyruvyl transferase (mura) genes from free-living actinobacteria to the parasitic chlamydiae.the chlamydiae are important human and animal pathogens which form a phylogentically distinct lineage within the bacteria. there is evidence that some genes in these obligate intracellular parasites have undergone lateral exchange with other free-living organisms. in the present work, we describe two interesting cases of lateral gene transfer between chlamydiae and actinobacteria, which have been identified based on the shared presence of conserved inserts in two important proteins. in the enzym ...200616830093
effects of atmospheric composition on respiratory behavior, weight loss, and appearance of camembert-type cheeses during chamber ripening.respiratory activity, weight loss, and appearance of camembert-type cheeses were studied during chamber ripening in relation to atmospheric composition. cheese ripening was carried out in chambers under continuously renewed, periodically renewed, or nonrenewed gaseous atmospheres or under a co(2) concentration kept constant at either 2 or 6% throughout the chamber-ripening process. it was found that overall atmospheric composition, and especially co(2) concentration, of the ripening chamber affe ...200616840643
microbiological and biochemical aspects of camembert-type cheeses depend on atmospheric composition in the ripening chamber.camembert-type cheeses were prepared from pasteurized milk seeded with kluyveromyces lactis, geotrichum candidum, penicillium camemberti, and brevibacterium aurantiacum. microorganism growth and biochemical dynamics were studied in relation to ripening chamber co(2) atmospheric composition using 31 descriptors based on kinetic data. the chamber ripening was carried out under 5 different controlled atmospheres: continuously renewed atmosphere, periodically renewed atmosphere, no renewed atmospher ...200616840644
structural and kinetic analyses of the h121a mutant of cholesterol oxidase.cholesterol oxidase is a monomeric flavoenzyme that catalyses the oxidation of cholesterol to cholest-5-en-3-one followed by isomerization to cholest-4-en-3-one. the enzyme from brevibacterium sterolicum contains the fad cofactor covalently bound to his121. it was previously demonstrated that the h121a substitution results in a approximately 100 mv decrease in the midpoint redox potential and a approximately 40-fold decrease in turnover number compared to wild-type enzyme [motteran, pilone, moll ...200616856877
[effect of synthetic surfactants on some biological properties of non-pathogenic species of the genus corynebacterium].the influence of cationic, anionic and non-ionic synthetic surfactants on some biological properties of different representatives of the following species corynebacterium glutamicum, corynebacterium ammoniagenes, corynebacterium vitaeruminis, corynebacterium variabile and strain corynebacterium sp. (brevibacterium stationis) ukm as-719 has been investigated. it has been shown that the action of surfactants is accompanied by the change of cell morphology and loss of ability for gram-staining, as ...200616869145
transport kinetics of ectoine, an osmolyte produced by brevibacterium epidermis.brevibacterium epidermis dsm 20659 is a halotolerant gram-positive bacterium which can synthesize the osmolyte, ectoine, but prefers to take it up from its environment. the present study revealed that b. epidermis is equipped with at least one transport system for ectoine, with a maximal transport velocity of 15.7 +/- 4.3 nmol/g cdw.min. the transport requires energy (atp) and is completely inhibited by the proton uncoupler, cccp. the ectoine uptake system is constitutively expressed at a basal ...200616900330
brevibacterium samyangense sp. nov., an actinomycete isolated from a beach sediment.a novel actinomycete, strain sst-8(t), was isolated from sand sediment of samyang beach in jeju, korea, and subjected to a polyphasic taxonomic study. the organism, which produced opaque, circular, yellow colonies, with a coryneform morphology, showed the following chemotaxonomic characteristics: meso-diaminopimelic acid as the diamino acid in the peptidoglycan, mk-8(h(2)) as the major menaquinone, phosphatidylglycerol as the only polar lipid, anteiso-c(15 : 0) and anteiso-c(17 : 0) as major fat ...200616902026
growth stimulation of brevibacterium sp. by siderophores.to assess which types of siderophores are typically produced by brevibacterium and how siderophore production and utilization traits are distributed within this genus.200616907814
isolation of an ethanol-tolerant endospore-forming gram-negative brevibacillus sp. as a covert contaminant in grape tissue cultures.to characterize the alcohol-surviving bacterial isolate arbg1 from in vitro grapes (vitis vinifera).200616968288
growth and colour development of some surface ripening bacteria with debaryomyces hansenii on aseptic cheese curd.the growth of five bacteria isolated from red-smear cheeses, brevibacterium aurantiacum, corynebacterium casei, corynebacterium variabile, microbacterium gubbeenense and staphylococcus saprophyticus in mixed cultures with debaryomyces hansenii on aseptic model cheese curd at 10 and 14 degrees c was investigated. at both temperatures, c. casei and micro. gubbeenense had a longer lag phase than c. variabile, brevi. aurantiacum and staph. saprophyticus. in all cultures, lactose was utilised first a ...200616978429
transcriptional profiling of bacteriophage bfk20: coexpression interrogated by "guilt-by-association" algorithm.global gene expression profiling of bacteriophage bfk20 infecting the industrial l-lysine producer brevibacterium flavum ccm 251 was performed using dna microarray. the relative gene expressions were measured in fourteen time samples collected during phage development. phage genes were classified as early, middle, late or unassigned based on complex expression patterns during infection. temporal classification of bfk20 genes was in concordance with previous predictions. however, proposed late re ...200717052739
transcriptional analysis of l-methionine catabolism in brevibacterium linens atcc9175.the expression of genes possibly involved in l-methionine and lactate catabolic pathways were performed in brevibacterium linens (atcc9175) in the presence or absence of added l-methionine. the expression of 27 genes of 39 selected genes differed significantly in l-methionine-enriched cultures. the expression of the gene encoding l-methionine gamma-lyase (mgl) is high in l-methionine-enriched cultures and is accompanied by a dramatic increase in volatile sulfur compounds (vsc) biosynthesis. seve ...200717225104
coenzyme precursor-assisted expression of a cholesterol oxidase from brevibacterium sp. in escherichia coli.the gene (chob(b)), encoding cholesterol oxidase from brevibacterium sp. cctcc m201008, was cloned and sequenced by pcr (genbank accession number: dq345780). the gene consists of 1653 base pairs and encodes a protein of 551 amino acids. chob(b) exhibited a homology of 98% with cholesterol oxidase gene from brevibacterium sterolicum atcc 21387. the cholesterol oxidase gene, cloned in the vector pet-28a, was over-expressed in escherichia coli bl21-codonplus (de3)-rp grown at 23 degrees c in luria- ...200717237971
controlled production of camembert-type cheeses: part iii role of the ripening microflora on free fatty acid concentrations.phenomena generating ffas, important flavour precursors, are significant in cheese ripening. in camembert-like cheeses, it was intended to establish the relationships between the dynamics of ffa concentrations changes and the succession of ripening microflora during ripening. experimental camembert-type cheeses were prepared in duplicate from pasteurised milk inoculated with kluyveromyces lactis, geotrichum candidum, penicillium camemberti, and brevibacterium aurantiacum under aseptic conditions ...200717291387
[a new polymer of 8,9-di-o-glucosylated 2-keto-3-deoxy-d-glycero-d-galacto-nonulosonic acid from a cell wall of brevibacterium casei aei ac-2114t].a polysaccharide containing the residues of 2-keto-3-deoxy-d-glycero-d-galacto-nonulosonic acid (kdn) was found in the cell wall of the brevibacterium casei strain aei ac-2114t . the polymer structure was elucidated by analyzing one-dimensional spectra of 1h and 13c nmr and bidimentional experiments 1h/13c-cosy, tocsy, 1h/13c-ghsqc, and 1h/13c-ghmbc. the polymer is built up of the 2--> 4-linked kdn residues substituted by beta-d-glcp residues at 8- and 9-hydroxyls; such a polymer with disubstitu ...200717375662
development of a large scale process for the conversion of polysialogangliosides to monosialotetrahexosylganglioside with a novel strain of brevibacterium casei producing sialidase.a bioconversion process of producing gm1 (monosialotetrahexosylganglioside) on an industrial scale was developed with a novel sialidase-producing strain brevibacterium casei. the sialidase hydrolyzed polysialogangliosides to produce gm1 but did not act on gm1. when brevibacterium casei was cultured in a synthetic medium containing crude pig brain gangliosides (10% w/v) at 30 degrees c for 24 h in a 50 l fermenter, most of the polysialogangliosides were converted to gm1. the content of gm1 was in ...200717415532
stability of the biodiversity of the surface consortia of gubbeen, a red-smear cheese.a total of 1,052 bacteria and 828 yeasts were isolated from the surface flora of 6 batches of gubbeen cheese made in 1996-1997 and 2002-2003. stability of the microflora was evaluated over time and also during ripening at 4, 10, and 16 d (batches 4, 5, and 6) or at 4, 16, 23, and 37 d (batches 1, 2, and 3). bacteria were identified using pulsed-field gel electrophoresis, repetitive extragenic palindromic-pcr, and 16s rrna gene sequencing, and yeasts were identified by fourier transform infrared ...200717430918
[cloning, sequence analysis and expression of anthranilate synthetase gene in corynebacterium pekinense].anthranilate synthetase (ec4.1.3.27;as) genes from wild-type corynebacterium pekinense as1.299 and its mutant pd-67 were cloned and sequenced. analysis of pcr fragments revealed that three orfs existed, which corresponded to trpl, trpe and trpg gene, respectively. six bases changes that resulted in the changes of five amino acids were found in the trpe structural gene of c. pekinense pd-67 and a single-base change that resulted in an amino acid substitution was found in the trpg structural gene ...200717436623
crystal structures of fe2+ dioxygenase superoxo, alkylperoxo, and bound product intermediates.we report the structures of three intermediates in the o2 activation and insertion reactions of an extradiol ring-cleaving dioxygenase. a crystal of fe2+-containing homoprotocatechuate 2,3-dioxygenase was soaked in the slow substrate 4-nitrocatechol in a low o2 atmosphere. the x-ray crystal structure shows that three different intermediates reside in different subunits of a single homotetrameric enzyme molecule. one of these is the key substrate-alkylperoxo-fe2+ intermediate, which has been pred ...200717446402
in vitro activity of linezolid and 12 other antimicrobials against coryneform bacteria.to compare the in vitro activity of linezolid with 12 other antimicrobials against 190 strains of the coryneform bacteria, including 60 strains of c. amycolatum, 30 of c. striatum, 30 of c. jeikeium, 10 of c. urealyticum, 20 of b. casei, 20 of d. hominis and 20 of t. otitidis.200717475450
transport capabilities of eleven gram-positive bacteria: comparative genomic analyses.the genomes of eleven gram-positive bacteria that are important for human health and the food industry, nine low g+c lactic acid bacteria and two high g+c gram-positive organisms, were analyzed for their complement of genes encoding transport proteins. thirteen to 18% of their genes encode transport proteins, larger percentages than observed for most other bacteria. all of these bacteria possess channel proteins, some of which probably function to relieve osmotic stress. amino acid uptake system ...200717490609
[pathway analysis for production of l-leucine by brevibacterium flavum tk0303].brevibacterium flavum is used for the production of a number of amino acids in the biotechnology industry. the yield of producing a metabolite is ultimately limited by the ability of the central metabolism and the desired biosynthesis pathway. pathway analysis is a very useful tool for metabolic engineering, which can be applied to increase the yield of a metabolite or channeling a metabolite into desired pathways. it does not require any kinetic parameters and only uses the stoichiometric equat ...200717552229
[taxonomic composition of bacteria associated with cultivated mollusks crassostrea lugubris and perna viridis and with the water of the gulf of nha trang lagoon, vietnam].one hundred and four strains of heterotrophic bacteria have been isolated and characterized from two species of bivalve mollusks cultivated in the gulf of nha trang (vietnam) and from the water of a mariculture farm. the isolates have been identified on the basis of morphological, physiological, biochemical, and chemotaxonomic properties, as well as by the content of g+c bases in dna. in the microflora of mollusks, vibrio alginolyticus was predominant; the pathogenic species v. harveyi and v. sp ...200717583223
purification and characterization of aldehyde dehydrogenase with a broad substrate specificity originated from 2-phenylethanol-assimilating brevibacterium sp. ku1309.phenylacetaldehyde dehydrogenase (padh) was purified and characterized from brevibacterium sp. ku1309, which can grow on the medium containing 2-phenylethanol as the sole carbon source. this enzyme was a homotetrameric protein with a subunit of 61 kda. the enzyme catalyzed the oxidation of aryl (benzaldehyde, phenylacetaldehyde, 3-phenylpropionaldehyde) and aliphatic (hexanal, octanal, decanal) aldehydes to the corresponding carboxylic acids using nad(+) as the electron acceptor. the padh activi ...200717619188
efficient cyclic system to yield ectoine using brevibacterium sp. jcm 6894 subjected to osmotic downshock.brevibacterium sp. jcm 6894 cells grown in the presence of 1.5-2.5 m nacl for 24 h at 30 degrees c were subjected to the osmotic downshock. downshocked cells after ectoine release were grown for further 24 h in the fresh medium with same salinity as before shock. when this cyclic system was applied to the strain jcm 6894, the amount of ectoine in the cells increased with an increase of incubation time, which indicates that the cells manipulated by the present conditions were enough active to sur ...200817696218
production of volatile aroma compounds by bacterial strains isolated from different surface-ripened french cheeses.twelve bacterial strains belonging to eight taxonomic groups: brevibacterium linens, microbacterium foliorum, arthrobacter arilaitensis, staphylococcus cohnii, staphylococcus equorum, brachybacterium sp., proteus vulgaris and psychrobacter sp., isolated from different surface-ripened french cheeses, were investigated for their abilities to generate volatile aroma compounds. out of 104 volatile compounds, 54 volatile compounds (identified using dynamic headspace technique coupled with gas chromat ...200717701035
corneal ulcers associated with aerosolized crack cocaine use.we report 4 cases of corneal ulcers associated with drug abuse. the pathogenesis of these ulcers and management of these patients are also reviewed.200717721298
growth characteristics of brevibacterium, corynebacterium, microbacterium, and staphylococcus spp. isolated from surface-ripened cheese.the growth characteristics of five bacteria, brevibacterium aurantiacum 1-16-58, corynebacterium casei dpc 5298(t), corynebacterium variabile dpc 5310, microbacterium gubbeenense dpc 5286(t), and staphylococcus saprophyticus 4e61, all of which were isolated from the surface of smear cheese, were studied in complex and chemically defined media. all of the coryneforms, except m. gubbeenense, grew in 12% salt, while b. aurantiacum and s. saprophyticus grew in 15% salt. all five bacteria assimilated ...200717921266
biochemical reference intervals and intestinal microflora of free-ranging ricord's iguanas (cyclura ricordii).caribbean iguanas, including the ricord's iguana (cyclura ricordii) are ranked as critically endangered on the international union of the conservation of nature (iucn) red list. the taxon is endemic to hispaniola, where it inhabits xeric lowlands and is found in a very limited range in two subpopulations in the southwestern area of the island. the goals of this study were to obtain and analyze plasma biochemical and intestinal microbiological data from ricord's iguanas in the subpopulation of th ...200717939350
ectoine as a natural component of food: detection in red smear cheeses.ectoine is a compatible solute accumulated in halophilic bacteria in response to high salt concentrations and offers protection from osmotic stress. the occurrence of compatible solutes is widespread among bacteria, yet ectoine has never been detected in foods. the use of an ectoine producing microorganism (brevibacterium linens) in the surface ripening of red smear cheeses led to the question whether ectoine can be found in cheese. therefore we examined samples from a variety of cheese manufact ...200717961287
microbial interactions within a cheese microbial community.the interactions that occur during the ripening of smear cheeses are not well understood. yeast-yeast interactions and yeast-bacterium interactions were investigated within a microbial community composed of three yeasts and six bacteria found in cheese. the growth dynamics of this community was precisely described during the ripening of a model cheese, and the lotka-volterra model was used to evaluate species interactions. subsequently, the effects on ecosystem functioning of yeast omissions in ...200817981942
[the diversity of alkane degrading bacteria in the enrichments with deep sea sediment of the south china sea].deep sea sediment samples of the south sea of china were used for isolation and biodiversity examination of hydrocarbon degrading bacterium. 48 isolates were obtained from the enrichments with hexadecane as the sole carbon sources. among them, 27 isolates were capable of degrading alkane; and 4 could produce biosurfactant significantly as determined by the surface tension measurement. 2 isolates belonging to dietzia maris lowered water surface tension to 33 mn/m. this is the first report about d ...200718062265
brevibacterium oceani sp. nov., isolated from deep-sea sediment of the chagos trench, indian ocean.two bacterial strains, designated bbh5 and bbh7(t), were isolated from a deep-sea sediment sample collected from the chagos trench of the indian ocean (1 degrees 06' s 7 degrees 31' e). based on their 16s rrna gene sequence similarity (99.9%), level of dna-dna relatedness (93%) and a number of similar phenotypic characteristics, the two strains are identified as representing the same species. their phylogenetically nearest neighbours, based on 16s rrna gene sequence similarity values (97.9-98.4% ...200818175683
relevance of the flavin binding to the stability and folding of engineered cholesterol oxidase containing noncovalently bound fad.the flavoprotein cholesterol oxidase (co) from brevibacterium sterolicum is a monomeric flavoenzyme containing one molecule of fad cofactor covalently linked to his69. the elimination of the covalent link following the his69ala substitution was demonstrated to result in a significant decrease in activity, in the midpoint redox potential of the flavin, and in stability with respect to the wild-type enzyme, but does not modify the overall structure of the enzyme. we used co as a model system to di ...200818218720
brevibacterium marinum sp. nov., isolated from seawater.a novel yellow-pigmented actinobacterium was isolated from seawater collected from hwasun beach in jeju, republic of korea. a comparative analysis of the 16s rrna gene sequence indicated that the organism, designated hfw-26(t), was closely related to members of the genus brevibacterium. as found for other species of the genus brevibacterium, strain hfw-26(t) possessed meso-diaminopimelic acid as the diagnostic cell-wall diamino acid, contained mk-8(h(2)) as the major menaquinone, contained polar ...200818218957
secretion of streptomyces mobaraensis pro-transglutaminase by coryneform bacteria.we previously reported on the secretion of streptomyces mobaraensis transglutaminase by corynebacterium glutamicum atcc13869 (formerly classified as brevibacterium lactofermentum). in the present work, we investigated whether any other coryneform bacteria showed higher productivity than c. glutamicum atcc13869. we found that most coryneform species secreted pro-transglutaminase efficiently. moreover, we confirmed that corynebacterium ammoniagenes atcc6872 produced about 2.5 g/l pro-transglutamin ...200818219481
the effect of cysteine on production of volatile sulphur compounds by cheese-ripening bacteria.the effect of cysteine on the ability of smear cheese-ripening bacteria (brevibacterium linens and arthrobacter spp) to produce volatile sulphur compounds (vsc) from methionine was studied. these bacteria were cultivated in a synthetic medium supplemented with various cysteine concentrations with or without methionine. cultures with only cysteine showed slightly lower levels of vsc produced and an unpleasant odour like rotten eggs, resulting from hydrogen sulphide production. the levels and prof ...200818262300
[purification and properties of an antimicrobial substance from marine brevibacillus laterosporus lh-1].an antimicrobial substance produced by brevibacillus laterosporus isolated from the sea sediment was purified and characterized. the antimicrobial substance was purified by ultrafiltration, deae-sepharose fast flow chromatography, cm-sepharose fast flow chromatography and hplc reversed phase column chromatography, and after the final purification step, one active fraction was obtained, designated r-1. the molecular weight (mw) was accurately determined by maldi-tof-ms as 1608.023 da. and its pi ...200718271253
assessment of microbial diversity in effluent treatment plants by culture dependent and culture independent approaches.microbial community structure of two distinct effluent treatment plants (etps) of pesticide and pharmaceutical industries was assessed and defined by (i) culture dependent and culture independent approaches on the basis of 16s rrna gene sequencing, (ii) diversity index analysis - operational taxonomic units (otus). a total of 38 and 44 bacterial otus having 85-99% similarity with the closest match in the database were detected among pharmaceutical and pesticide sludge samples, respectively. fift ...200818280146
commercial ripening starter microorganisms inoculated into cheese milk do not successfully establish themselves in the resident microbial ripening consortia of a south german red smear cheese.production of smear-ripened cheese critically depends on the surface growth of multispecies microbial consortia comprising bacteria and yeasts. these microorganisms often originate from the cheese-making facility and, over many years, have developed into rather stable, dairy-specific associations. while commercial smear starters are frequently used, it is unclear to what degree these are able to establish successfully within the resident microbial consortia. thus, the fate of the smear starters ...200818281427
detoxification of toxic heavy metals by marine bacteria highly resistant to mercury.pollution in industrial areas is a serious environmental concern, and interest in bacterial resistance to heavy metals is of practical significance. mercury (hg), cadmium (cd), and lead (pb) are known to cause damage to living organisms, including human beings. several marine bacteria highly resistant to mercury (bhrm) capable of growing at 25 ppm (mg l(-1)) or higher concentrations of mercury were tested during this study to evaluate their potential to detoxify cd and pb. results indicate their ...200818288535
[decrease of phosphate concentration in the medium by brevibacterium casei cells].brevibacteria able to decrease phosphate concentration in the medium are of interest for the study of the role of bacteria in the phosphorus cycle and for development of biotechnology of phosphate removal from waste. brevibacterium casei, brevibacterium linens, and brevibacterium epidermidis grown in media with initial phosphorus concentrations of 1-11 mm were shown to decrease its concentration by 90%. the composition of the incubation medium required for b. casei to carry out this process was ...200718297865
biodegradation of gaseous styrene by brevibacillus sp. using a novel agitating biotrickling filter.a novel biofilter with an agitator to control excessive biomass accumulation, the agitating biotrickling filter (abtf) system, was developed for treatment of gaseous styrene using brevibacillus sp. as the sole microorganism the abtf exhibited an elimination capacity of 3 kg styrene m(-3) day(-1). after 110 days, the biodegradation efficiency decreased because of the clogging. the excess biomass was effectively removed by agitation. after the first agitation step, 42.4 g biomass was eliminated, a ...200818317704
brevibacterium album sp. nov., a novel actinobacterium isolated from a saline soil in china.a novel gram-positive, rod-shaped actinobacterium, designated strain yim 90718(t), was isolated from a saline soil in xinjiang province, north-west china, and subjected to polyphasic taxonomy. the peptidoglycan type was a1gamma and the cell-wall sugars contained galactose. phospholipids were phosphatidylglycerol and diphosphatidylglycerol. the predominant menaquinone was mk-8(h(2)). the major fatty acids were anteiso-c(15 : 0), anteiso-c(17 : 0) and iso-c(15 : 0). all of these chemotaxonomic dat ...200818319457
application of pcr-sscp for molecular epidemiological studies on the exposure of farm children to bacteria in environmental dust.the environmental exposure of farm children to microorganisms in dust has become a focus of interest, since microbial exposure on farms has been related to a reduced prevalence of asthma and atopic diseases in children. previous studies almost exclusively focused on the determination of microbial counts using conventional culturing or the determination of microbial compounds i.e. endotoxins. in this study pcr-sscp (single-strand conformation polymorphism) was modified for characterising bacteria ...200818321601
cloth colorization caused by microbial biofilm.in this study, cloth disfeaturement was investigated biologically. to clarify whether or not microbes can cause cloth disfeaturement, and to identify the microbes causing the disfeaturement, worn cloth samples were incubated on sweat-ingredient agar medium. non-sterilized cloth samples became yellow-colored during incubation, and bacterial strains belonging to the genera bacillus, brevibacterium, kocuria, micrococcus and staphylococcus were isolated from the yellow-colored parts. two major isola ...200818359206
co-metabolic degradation of bensulfuron-methyl in laboratory conditions.the present study deals with the degradation of bensulfuron-methyl by microorganisms cultured with different sources of carbon, nitrogen and phosphorus. addition of carbon source accelerated the degradation of bensulfuron-methyl under co-metabolism process. sodium lactate was the best carbon source for the degradation of bensulfuron-methyl, compared to other carbon sources studied, and the degradation ratio of bensulfuron-methyl reached 79.5%, whereas only 34.6 and 29.7% were removed in the pres ...200818417283
a comparison of the reaction mechanisms of iron- and manganese-containing 2,3-hpcd: an important spin transition for manganese.homoprotocatechuate (hpca) dioxygenases are enzymes that take part in the catabolism of aromatic compounds in the environment. they use molecular oxygen to perform the ring cleavage of ortho-dihydroxylated aromatic compounds. a theoretical investigation of the catalytic cycle for hpca 2,3-dioxygenase isolated from brevibacterium fuscum (bf 2,3-hpcd) was performed using hybrid dft with the b3lyp functional, and a reaction mechanism is suggested. models of different sizes were built from the cryst ...200818458966
r-enantioselective hydrolysis of 2,2-dimethylcyclopropanecarboxamide by amidase from a newly isolated strain brevibacterium epidermidis zjb-07021.to isolate new micro-organisms with r-stereospecific amidase activity and to examine their potential as biocatalysts in enantioselective hydrolysis of 2,2-dimethylcyclopropanecarboxamide (1).200818492048
swapping metals in fe- and mn-dependent dioxygenases: evidence for oxygen activation without a change in metal redox state.biological o(2) activation often occurs after binding to a reduced metal [e.g., m(ii)] in an enzyme active site. subsequent m(ii)-to-o(2) electron transfer results in a reactive m(iii)-superoxo species. for the extradiol aromatic ring-cleaving dioxygenases, we have proposed a different model where an electron is transferred from substrate to o(2) via the m(ii) center to which they are both bound, thereby obviating the need for an integral change in metal redox state. this model is tested by usin ...200818492808
the shortest wire. 200818508968
purification and characterization of thermostable h2o2-forming nadh oxidase from 2-phenylethanol-assimilating brevibacterium sp. ku1309.a cytoplasmic nadh oxidase (nox) was purified from a soil bacteria, brevibacterium sp. ku1309, which is able to grow in the medium containing 2-phenylethanol as the sole source of carbon under an aerobic condition. the enzyme catalyzed the oxidation of nadh to nad+ involving two-electron reduction of o2 to h2o2. the molecular weight of the enzyme was estimated to be 102 kda by gel filtration and 57 kda by sds-page, which indicates that the nox was a homodimer consisting of a single subunit. the ...200818521590
production of nadp by immobilized cells with nad kinase.by the radiation-copolymerization method with polyethylene glycoldimethacrylate (pgd) as a main polymerizable reagent, microbial cells of brevibacterium ammoniagenes were immobilized with high specific activity of nad kinase and high mechanical strength. the reagents used for the immobilization such as pgd, polyvinyl alcohol (pva), and n,n'-methylenebisacrylamide (bis) did not inversely affect the enzyme activity. freezing and irradiation treatment of the cell-reagent solution did not inactivate ...198218546376
in situ global method for measurement of oxygen demand and mass transfer.two aerobic microorganisms, saccharomycopsis lipolytica and brevibacterium lactofermentum, have been used in a study of mass transfer and oxygen uptake from a global perspective, using a closed gas system. oxygen concentrations in the gas and liquid were followed using oxygen electrodes; the results allowed for easy calculation of in situ oxygen transport. the cell yields on oxygen for s. lipolytica and b. lactofermentum were 1.01 and 1.53 g/g, respectively. the mass transfer coefficient was est ...199818576018
the use of the maharanobis and modified distances for the improvement of simulation of glutamic acid production.a modified simulation procedure based on a statistical approach was investigated. the procedure predicts the time course of fed-batch culture for glutamic acid production by a temperature-sensitive strain of brevibacterium flavum. the statistical approach requires only a data base of state points obtained in experiments, and not perfect identification of fermentation models. the simulation procedure is based on regression analysis to estimate specific rate parameters of system equations using th ...198918587900
biotransformation of acrylonitrile to acrylamide using immobilized whole cells of brevibacterium ch1 in a recycle fed-batch reactor.acrylamide was produced from acrylonitrile using immobilized brevibacterium ch1 cells that were isolated from soil and found to possess nitrile hydratase activity. the reaction conditions and stability of the enzyme activity were studied. the conversion yield was nearly 100%, including a trace amount of acrylic acid. this strain showed strong activity of nitrile hydratase toward acrylonitrile and extremely low activity of amidase toward acrylamide. a packed bed reactor was operated in a fed-batc ...198918588114
genome sequence of the lytic bacteriophage p1201 from corynebacterium glutamicum nchu 87078: evolutionary relationships to phages from corynebacterineae.p1201 is a lytic corynephage of corynebacterium glutamicum nchu 87078. its genome consists of a linear double-stranded dna molecule of 70,579 base pairs, with 3'-protruding cohesive ends of ten nucleotides. we have identified 69 putative open reading frames, including three apparent genes (thymidylate synthase, terminase, and rnr alpha subunit genes) that are interrupted by an intein. protein-splicing activities of these inteins were demonstrated in escherichia coli. three structural proteins in ...200818599103
enzyme-catalyzed oxidation of cholesterol in physically characterized water-in-oil microemulsions.the enzymatic conversion of cholesterol to cholestenone by cholesterol oxidase (brevibacterium sp.)in reversed micelles in a system composed of aot/isooctane/water/cholesterol has been examined. the catalytic activity of the enzyme was correlated with the physicochemical properties of water in water-in-oil (w/o) microemulsion systems. in a system consisting of 3 wt % aot in isooctane, reversed micelles started to form as the [h(2)o]/[aot] (e.g., the w(0)) ratio increased above 4-5. the formation ...199218600934
cloning and characterization of the gene cluster for biosynthesis of ectoine from nesterenkonia halobia dsm 20541.the ectabc genes encoding the biosynthesis of ectoine were identified from nesterenkonia halobia dsm 20541. the intergenic regions of the ectabc genes from n. halobia dsm 20541 were more loosely spaced than those that had been reported before. the amino acid sequence deduced from ectabc of the strain was highly homologous to the ectabc of brevibacterium linens bl2 (ecta 50%, ectb 70%, and ectc 68% identities). the osmoprotection of ectabc was studied in the escherichia coli knabc and e. coli xl1 ...200818604501
inhibition of microbial growth and metabolism by excess turbulence.excess turbulence caused by high-intensity stirring inhibited microbial growth and metabolism. in stirred tank bioreactors, the growth rate and lysine biosynthesis decreased in brevibacterium flavum beyond 900 rpm, the growth rate of trichoderma reesei on wheat straw beyond 150 rpm, and the growth rate of saccharomyces cerevisae beyond 800 rpm. the term turbohypobiosis was introduced to describe this inhibition. turbohypobiosis was characterized by a stress factor f(str) expressing the interacti ...199118604814
air slugs entrapped cross-flow filtration of bacterial suspensions.a novel cross-flow technique for membrane filtration of bacterial cell suspensions was established. this is an air slugs entrapped cross-flow method in which air slugs were generated by introducing air into the cross-flow stream. as air slugs moved along with cross-flow, the disturbance of cell sublayer formation on membrane surface was enhanced. as a consequence, filtration flux was improved and stabilized. the effect of air slugs on improving filtration flux was more pronounced in filtering gr ...199318609583
on the oxygen reactivity of flavoprotein oxidases: an oxygen access tunnel and gate in brevibacterium sterolicum cholesterol oxidase.the flavoprotein cholesterol oxidase from brevibacterium sterolicum (bco) possesses a narrow channel that links the active center containing the flavin to the outside solvent. this channel has been proposed to serve for the access of dioxygen; it contains at its "bottom" a glu-arg pair (glu-475-arg-477) that was found by crystallographic studies to exist in two forms named "open" and "closed," which in turn was suggested to constitute a gate functioning in the control of oxygen access. most muta ...200818614534
fermentation and recovery of glutamic acid from palm waste hydrolysate by ion-exchange resin column.glutamic acid produced from palm waste hydrolysate by fermentation with brevibacterium lactofermentum atcc 13869 is produced with a remarkably high yield compared with that produced from pure glucose as a carbon source. the produce yield is 70 g/l with glucose, wherease, when palm waste hydrolysate is the fermentation medium in the same bioreactor under same conditions, it is 88 g/l. the higher yield may be attributed to the fact that this organism has the ability to convert sugars other than on ...199518623521
identities of arthrobacter spp. and arthrobacter-like bacteria encountered in human clinical specimens.after the initial description of arthrobacter spp. isolated from clinical specimens in the mid-1990s, very few further reports on arthrobacter spp. have appeared in the clinical microbiology literature. the aim of the present study was to elucidate the distribution of arthrobacter spp. and arthrobacter-like bacteria encountered in clinical specimens by studying 50 consecutively isolated or received strains of large-colony-forming, whiteish-grayish, non-cheese-like-smelling, nonfermentative gram- ...200818650355
novel methylotrophic bacteria isolated from the river thames (london, uk).enrichment and elective culture for methylotrophs from sediment of the river thames in central london yielded a diversity of pure cultures representing several genera of gram-negative and gram-positive bacteria, which were mainly of organisms not generally regarded as typically methylotrophic. substrates leading to successful isolations included methanol, monomethylamine, dimethylamine, trimethylamine, methanesulfonate and dimethylsulfone. several isolates were studied in detail and shown by the ...200818681896
optimization of trehalose production by a novel strain brevibacterium sp. sy361.trehalose production by a novel strain of brevibacterium sp. sy361 was optimized in submerged fermentation. different chemical and physical parameters such as carbon and nitrogen sources, inoculum level, initial ph, incubation temperature, aeration and time-course of fermentation, were studied in order to increase trehalose productivity. an optimal production medium containing 3% (w/v) glucose, 0.9% (v/v) corn steep liquor, 0.5% (w/v) kh(2)po(4) and 0.4% (w/v) mgso(4).7 h(2)o was found suitable ...200818759225
inhibition of photosynthesis in cyanobacteria and plankton algae by the bacterium brevibacillus laterosporus metabolites. 200818853767
identification of aerobic gut bacteria from the kala azar vector, phlebotomus argentipes: a platform for potential paratransgenic manipulation of sand flies.visceral leishmaniasis is an understudied parasitic disease responsible for significant global morbidity and mortality. we are presently investigating a method of disease prevention termed paratransgenesis. in this approach, symbiotic or commensal bacteria are transformed to produce anti-leishmania molecules. the transformed bacteria are delivered back to sand flies to inactivate the parasite within the vector itself. in this study, we identified 28 distinct gut microorganisms from phlebotomus a ...200819052297
maturing dynamics of surface microflora in fontina pdo cheese studied by culture-dependent and -independent methods.to study the evolution of rind microbial communities in fontina pdo cheese.200919054234
l-lysine fermentation.amino acids are the basic bioelements of proteins, which are the most important macromolecules for the functions of humans and animals. out of the 20 l-amino acids, ecumenically found in most of living organisms, l-lysine is one of the 9 amino acids which are essential for human and animal nutrition. l-lysine is useful as medicament, chemical agent, food material (food industry) and feed additive (animal food). its demand has been steadily increasing in recent years and several hundred thousands ...200719075830
structural basis of enzyme encapsulation into a bacterial nanocompartment.compartmentalization is an important organizational feature of life. it occurs at varying levels of complexity ranging from eukaryotic organelles and the bacterial microcompartments, to the molecular reaction chambers formed by enzyme assemblies. the structural basis of enzyme encapsulation in molecular compartments is poorly understood. here we show, using x-ray crystallographic, biochemical and em experiments, that a widespread family of conserved bacterial proteins, the linocin-like proteins, ...200819172747
lines of evidence for horizontal gene transfer of a phenazine producing operon into multiple bacterial species.phenazines are secondary metabolites with broad-spectrum antibiotic activity against bacteria, fungi, and eukaryotes. in pseudomonad species, a conserved seven-gene phenazine operon (phzabcdefg) is required for the conversion of chorismic acid to the broad-spectrum antibiotic phenazine-1-carboxylate. previous analyses of genes involved in phenazine production from nonpseudomonad species uncovered a high degree of sequence similarity to pseudomonad homologues. the analyses undertaken in this stud ...200919189039
entrapment of live microbial cells in electropolymerized polyaniline and their use as urea biosensor.the lyophilized biomass of bacterium brevibacterium ammoniagenes was immobilized in polystyrene sulphonate-polyaniline (pss-pani) conducting polymer on a pt twin wire electrode by potentiostatic electropolymerization. the bacterial cells retained their viability as well as urease activity under entrapped state, as confirmed with bacterial live-dead fluorescent assay and enzymatic assays. the entrapped cells were visualized using scanning electron microscope. the immobilized cells were used as a ...200919230647
heterologous production of methionine-gamma-lyase from brevibacterium linens in lactococcus lactis and formation of volatile sulfur compounds.the conversion of methionine to volatile sulfur compounds (vscs) is of great importance in flavor formation during cheese ripening and is the focus of biotechnological approaches toward flavor improvement. a synthetic mgl gene encoding methionine-gamma-lyase (mgl) from brevibacterium linens bl2 was cloned into a lactococcus lactis expression plasmid under the control of the nisin-inducible promoter pnisa. when expressed in l. lactis and purified as a recombinant protein, mgl was shown to degrade ...200919251895
development of multi-component transplant mixes for suppression of meloidogyne incognita on tomato (lycopersicon esculentum).the effects of combinations of organic amendments, phytochemicals, and plant-growth promoting rhizobacteria on tomato (lycopersicon esculentum) germination, transplant growth, and infectivity of meloidogyne incognita were evaluated. two phytochemicals (citral and benzaldehyde), three organic amendments (pine bark, chitin, and hemicellulose), and three bacteria (serratia marcescens, brevibacterium iodinum, and pseudomonas fluorescens) were assessed. increasing rates of benzaldehyde and citral red ...200219265957
do milking practices influence the bacterial diversity of raw milk?the link between milk production practices and bacterial diversity of 67 raw milks from dairy farms in the savoie and haute-savoie regions of france was studied by single strand conformation polymorphism (sscp) analysis. the milking practices and the cleanliness of different parts of the cow housing were evaluated. the sscp bacterial profiles allow to classify the 67 milks into three groups: group a was characterised by a majority of gram-positive non-lactic acid bacteria (corynebacterineae and ...200919269573
bacterial communities in a crude oil gathering and transferring system (china).bacterial communities in crude oil and oil field production water samples from an oil gathering and transferring system in changqing oil field in china were investigated by 16s rrna denaturing gradient gel electrophoresis (dgge) analysis followed by gene cloning and sequencing. dgge profiles showed that bacterial communities are far more rich in the water samples than that in the crude oil samples, and that bacteria related to ochrobactrum sp. and stenotrophomonas sp. were detected in all crude ...200919351561
brevibacillus brevis peritonitis.we present what we believe is the first case of brevibacillus (bacillus) brevis peritonitis in a patient with hepatocellular carcinoma, possibly caused by the ingestion of fermented foods containing b. brevis spores. this case also demonstrates a pattern of antibiotic susceptibility with differing in vitro and in vivo bactericidal efficacy.200919402209
effects of proteus vulgaris growth on the establishment of a cheese microbial community and on the production of volatile aroma compounds in a model cheese.to investigate the impact of proteus vulgaris growth on a multispecies ecosystem and on volatile aroma compound production during cheese ripening.200919426267
investigation of protease-mediated cuticle-degradation of nematodes by using an improved immunofluorescence-localization method.in order to facilitate the understanding of the actual process of enzyme-based degradation of nematodes, we visualized the localization of blg4, a cuticle-degrading protease from the nematophagous bacterium brevibacillus laterosporus g4, on nematode cuticle by using an improved immuno-labeled fluorescent method. live nematodes, heat-killed nematodes and extracted nematode cuticles were exposed to the protease, and the localization of the protease and the resulting tissue degradation and destruct ...200919435598
real-time nanomechanical and topographical mapping on live bacterial cells-brevibacterium casei under stress due to their exposure to co2+ ions during microbial synthesis of co3o4 nanoparticles.the study of elastic properties of microbial and mammalian cells using atomic force microscopy, with force-sensitivity as high as pico-newtons and spatial resolution of a few nanometers, is proving to be a great tool for the real-time observation of the effects of drugs, biomolecules, metal ions, and nanoparticles on cell physiology in their natural environment. it has been shown that the young's modulus of the cell surfaces is extremely sensitive to the surrounding environment. recently, a broa ...200919438181
[phylogenetic diversity of cultivable bacteria associated with a sea anemone from coast of the naozhou island in zhanjiang, china].to understand the diversity of cultivable bacteria isolated from a sea anemone collected from the coastal water of the naozbou island in the leizhou bay on south china sea.200919445182
combined dissolved oxygen and ph control strategy to improve the fermentative production of l-isoleucine by brevibacterium lactofermentum.the effect of both dissolved oxygen (do) and ph on l: -isoleucine production by batch culture of brevibacterium lactofermentum was investigated. a two-stage agitation speed control strategy was developed, and the isoleucine production reached 23.3 g l(-1) in a relative short time (52 h), increased by 11.6% compared to the results obtained in the single agitation speed control process. in order to make sure whether the combination of do and ph control can boost the production by a mutual effect, ...201019449037
microbial ecology of gorgonzola rinds and occurrence of different biotypes of listeria monocytogenes.in this study we investigated the microbiota of gorgonzola rinds and maturing shelf swabs collected in 5 different maturing cellars in the northwest part of italy, in association with the detection and characterization of listeria monocytogenes. culture-dependent and -independent methods were performed in order to profile the main microbial populations present on the rinds and in the maturing shelves and species-specific pcr and pulsed field gel electrophoresis (pfge) were used to identify and t ...200919477544
detection of hemolytic bacteria from palythoa caribaeorum (cnidaria, zoantharia) using a novel palytoxin-screening assay.palytoxin (ptx), one of the most potent and chemically complex marine toxins, is predominantly found in zoanthid corals and sporadically in dinoflagellates. its biosynthesis and metabolic pathways are largely unknown. however, the widespread occurrence of the toxin in phylogenetically distinct marine organisms is consistent with its production by microorganisms and subsequent accumulation in the food chain. to investigate a possible microbial origin, bacteria from two zoanthid corals (palythoa c ...200919504172
evaluation of antibacterial activity, phenol and flavonoid contents of thespesia populnea flower extracts.flavonoids which were reported as having many pharmacological activities, antimicrobial, antioxidant, cytotoxic, chemoprevention activities and they possess strong antiproliferative effects related to inhibition of cell cycle progression and apoptosis induction. on the basis of this thespesia populnea (l.) sol. ex correa (family-malvaceae) was selected and it is having the major composition of flavonoids and the antibacterial activity of methanolic extract of thespesia populnea flowers was inves ...200919553175
brevibacterium massiliense sp. nov., isolated from a human ankle discharge.gram-positive, non-spore-forming rods, strain 5401308t, were isolated from a human ankle discharge. based on cellular morphology and the results of biochemical testing, this strain was tentatively identified as an undescribed member of the genus brevibacterium. the major fatty acids were anteiso-c15:0 (45.3%), anteiso-c17:0 (19.2%) and iso-c15:0 (18.3%). phylogenetic analysis based on 16s rrna gene sequence comparison showed that the bacterium was closely related to the type strains of brevibact ...200919567570
purification, immobilization, and characterization of nattokinase on phb nanoparticles.in this study, nattokinase was purified from bacillus subtilis using ion exchange chromatography and immobilized upon polyhydroxybutyrate (phb) nanoparticles. a novel strain isolated from industrial dairy waste was found to synthesize polyhydroxyalkanoates (pha) and the strain was identified as brevibacterium casei srkp2. pha granules were extracted from 48 h culture and the ft-ir analysis characterized them as phb, a natural biopolymer from b. casei. nanoprecipitation by solvent displacement te ...200919608412
identification of brevibacteriaceae by multilocus sequence typing and comparative genomic hybridization analyses.multilocus sequence typing with nine selected genes is shown to be a promising new tool for accurate identifications of brevibacteriaceae at the species level. a developed microarray also allows intraspecific diversity investigations of brevibacterium aurantiacum showing that 13% to 15% of the genes of strain atcc 9174 were absent or divergent in strain bl2 or atcc 9175.200919648361
brevibacterium pityocampae sp. nov., isolated from caterpillars of thaumetopoea pityocampa (lepidoptera, thaumetopoeidae).this work deals with the taxonomic study of a bacterium, strain tp12(t), isolated from caterpillars of the pine processionary moth (thaumetopoea pityocampa denis & schiffermüller, 1775; lepidoptera, thaumetopoeidae). the isolate was assigned to the genus brevibacterium on the basis of a polyphasic taxonomic study, including morphological and biochemical characteristics, 16s rrna gene sequence analysis, fatty acid analysis and dna g+c content. the highest 16s rrna gene sequence similarity to this ...201019651741
assignment of brevibacterium stationis (zobell and upham 1944) breed 1953 to the genus corynebacterium, as corynebacterium stationis comb. nov., and emended description of the genus corynebacterium to include isolates that can alkalinize citrate.brevibacterium stationis atcc 14403(t), corynebacterium ammoniagenes atcc 6872 and two clinical isolates were found to form a single taxon group consistent with the genus corynebacterium, designated here as corynebacterium stationis comb. nov. the type strain of corynebacterium stationis is atcc 14403(t) =ccug 43497( t) =cip 104228(t) =dsm 20302(t) =nbrc 12144(t) =jcm 11611(t) =vkm b-1228(t). these strains can utilize citrate; therefore, inclusion of c. stationis requires that the description of ...201019661509
brevibacterium sandarakinum sp. nov., isolated from a wall of an indoor environment.a gram-stain-positive, rod-shaped, non-endospore-forming, orange-pigmented (coloured) actinobacterium (01-je-003(t)) was isolated from the wall of an indoor environment primarily colonized with moulds. on the basis of 16s rrna gene sequence similarity studies, strain 01-je-003(t) was shown to belong to the genus brevibacterium and was most similar to the type strains of brevibacterium picturae (98.8 % similarity), brevibacterium marinum (97.3 %) and brevibacterium aurantiacum (97.2 %). chemotaxo ...201019661519
synthesis of phb nanoparticles from optimized medium utilizing dairy industrial waste using brevibacterium casei srkp2: a green chemistry approach.polyhydroxyalkanoates (phas) are natural, biodegradable polymers accumulated by bacteria under nutritional exhausted condition where carbon source is in excess. a gram positive bacterium (designated strain srkp2) that potentially accumulated polyhydroxybutyrate (phb) was isolated from dairy industrial waste. from its morphological and physiological properties and nucleotide sequence of its 16s rrna, it was suggested that strain srkp2 was similar to brevibacterium casei. phas were synthesized fro ...200919700268
isolation and identification of novel microcystin-degrading bacteria.of 31 freshwater bacterial isolates screened using the biolog mt2 assay to determine their metabolism of the microcystin lr, 10 were positive. phylogenetic analysis (16s rrna) identified them as arthrobacter spp., brevibacterium sp., and rhodococcus sp. this is the first report of microcystin degraders that do not belong to the proteobacteria.200919734339
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