Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| streptococcal r plasmid pip501: endonuclease site map, resistance determinant location, and construction of novel derivatives. | the streptococcal resistance plasmid pip501 (30 kilobase pairs [kb]) encodes resistance to chloramphenicol (cmr) and erythromycin (emr) and is capable of conjugative transfer among numerous streptococcal species. by using a streptococcal host-vector recombinant dna system, the cmr and emr determinants of pip501 were localized to 6.3-kb hindiii and 2.1-kb hindiii-avai fragments, respectively. pip501 was lost at a frequency of 22% in streptococcus sanguis cells grown at 42 degrees c but was stable ... | 1983 | 6304011 |
| plasmid transfer in pediococcus spp.: intergeneric and intrageneric transfer of pip501. | transfer of the broad-host-range resistance plasmid pip501 from streptococcus faecalis to pediococcus pentosaceus and pediococcus acidilactici occurred between cells immobilized on nitrocellulose filters in the presence of dnase. expression of the pip501-linked erythromycin and chloramphenicol resistance determinants was observed in transconjugants. intrageneric transfer of pip501 from a p. pentosaceus donor to various pediococcal recipients occurred at frequencies of 10(-4) to 10(-7) transconju ... | 1983 | 6311098 |
| an in-vitro study of the role of sucrose and interactions between oral bacteria in possible mechanisms of dental plaque formation. | the adherence of streptococci to hydroxyapatite spheroids (has) depended upon the strain used, the fermentable carbon source available during bacterial growth, the number of times a clinical isolate had been subcultured on laboratory media and the pre-treatment of the has. sucrose greatly stimulated the secondary colonization of streptococcus mutans strain 3209 on has pre-equilibrated with this strain, but no similar effect was observed with glucose. pre-equilibration of has with streptococcus s ... | 1983 | 6314950 |
| adherence of oral bacteria to chemically modified hydroxyapatite. | 1984 | 6317176 | |
| novel shuttle plasmid vehicles for escherichia-streptococcus transgeneric cloning. | a novel plasmid vector that is able to replicate both in escherichia coli and in streptococcus sanguis is described. this 9.2-kb plasmid, designated pva856, carries cmr, tcr, and emr determinants that are expressed in e. coli. only the emr determinant is expressed in s. sanguis. both the cmr and the tcr of pva856 may be insertionally inactivated. this plasmid affords several different cleavage-ligation strategies for cloning in e. coli followed by subsequent introduction of chimeras into s. sang ... | 1983 | 6319229 |
| adherence of streptococcus sanguis to saliva-coated hydroxyapatite: evidence for two binding sites. | the characteristics of bacterial adherence to saliva-coated hydroxyapatite were examined for a salivary aggregating strain of streptococcus sanguis, strain 12, and for its nonaggregating variant, strain 12na. both strains were found to adhere in similar numbers to saliva-coated hydroxyapatite that had been preincubated at 4 degrees c overnight. preincubation of saliva-coated hydroxyapatite overnight at 37 degrees c reduced subsequent adherence of s. sanguis 12 by approximately 10%, whereas adher ... | 1984 | 6319287 |
| physical and genetic analyses of streptococcal plasmid pam beta 1 and cloning of its replication region. | plasmid pam beta 1, originally isolated from streptococcus faecalis ds5, mediates resistance to the mls (macrolide, lincosamide, and streptogramin b alpha) group of antibiotics. a restriction endonuclease map of the 26.5-kilobase (kb) pam beta 1 molecule was constructed by using the enzymes avai, hpaii, ecori, pvuii, kpn1, bsteii, hpai, hhai, and hindiii. a comparison of this map to those of four independently isolated deletion derivatives of pam beta 1 located the mls resistance determinant wit ... | 1984 | 6319361 |
| microbial pathogenicity in oral soft tissue diseases. | recent work implicating microbial factors in the pathogenesis of several oral soft tissue diseases is discussed. these conditions include recurrent aphthous ulceration, oral cancer, sjogren's syndrome, and the oral lesions of aids (the acquired immune deficiency syndrome). this paper reviews some recent work on these topics, with emphasis on investigations in our laboratory. | 1984 | 6321576 |
| optimization of an hydroxyapatite adhesion assay for streptococcus sanguis. | previous studies have compared the adhesion of [3h]thymidine-labeled streptococcus sanguis to saliva-coated hydroxyapatite (sha) and buffer-coated hydroxyapatite (ha) beads. although the hypotonic buffer used in these assays was adjusted to simulate saliva, it does not necessarily provide the optimal parameters for the quantitative estimate of adhesion under in vitro conditions. optimization is necessary to provide the maximum sensitivity of the assay for detecting the effects of various salivas ... | 1984 | 6325348 |
| [effect of solid substrates of microbial colony development]. | bacteria have been found to grow differently at contact sites to both organic and anorganic solids as on pure agar media. of 16 ad hoc selected bacterial strains, the following species (arranged in decreasing order) responded with stronger colony formation: bacillus subtilis, b. cereus, streptococcus sanguis, s. saprophyticus, s. faecalis, escherichia coli, micrococcus flavus, actinomyces viscosus, veillonella sp., enterobacter liquefaciens, yersinia enterocolitica. lactobacillus casei behaved n ... | 1984 | 6326398 |
| adherence of oral streptococci: evidence for nonspecific adsorption to saliva-coated hydroxylapatite surfaces. | it is proposed that binding of oral streptococci to saliva-coated hydroxylapatite (sha) surfaces is a multifactorial process involving both specific and nonspecific receptors. in this context, specific binding is described as a high-affinity, saturable interaction between the cell and binding surface. conversely, nonspecific binding is considered to be a nonsaturable, generalized, low-affinity reaction. experimental differentiation of specific binding from nonspecific binding was achieved with a ... | 1984 | 6327530 |
| application of a competition model to the growth of streptococcus mutans and streptococcus sanguis in binary continuous culture. | streptococcus mutans 6715-15 and streptococcus sanguis 10558 were grown together in continuous culture with glucose as the limiting carbon source. the relationship of growth rate to substrate concentration was determined for pure cultures of each organism in continuous and batch cultures. a model based on competition for a growth-limiting substrate (glucose) was used to predict the proportions of each organism when grown in binary cultures. the results indicate that interactions other than compe ... | 1983 | 6344790 |
| occurrence and nature of bacterial iga proteases. | 1983 | 6347000 | |
| inhibition of bacterial iga proteases by human secretory iga and serum. | 1983 | 6347001 | |
| of human dental plaque bacteria, actinomyces viscosus accelerates immunoglobulin a protease secretion in streptococcus sanguis. | 1983 | 6347146 | |
| quantitative screening of clinical isolates for immunoglobulin a protease production. | the production of immunoglobulin a (iga) protease is a potentially useful marker in differentiating pathogenic from nonpathogenic species of clinical isolates; however, current quantitative assay methods are too tedious for routine application. a simple quantitative method was developed to screen clinical isolates for iga protease production. this method is based on the specificity of reaction between iga and alpha chain-specific antiserum in an immunochemistry analyzer (beckman instruments, inc ... | 1983 | 6352734 |
| cleavage of protein a-binding iga1 with iga1 protease from streptococcus sanguis. | protein a-binding fractions of two iga1 myeloma proteins failed to produce fc fragments on digestion with iga1 protease from streptococcus sanguis. a polymeric protein a-binding iga1 fraction yielded a protein a-non-binding monomer, which was further cleaved into fab fragments but it did not yield fc fragments. the protein a-binding fraction of a monomeric iga1 yielded an iga molecule lacking one fab fragment. subsequently, the remaining part of its cleaved alpha chain was degraded. further dige ... | 1983 | 6358000 |
| aggregation of streptococcus sanguis by a neuraminidase-sensitive component of serum and crevicular fluid. | a number of strains of streptococcus sanguis were found to aggregate in nonimmune serum and in crevicular fluid. all strains which aggregated in serum also aggregated in saliva, but some strains which aggregated in saliva did not aggregate in serum. aggregation was destroyed by treatment of serum or crevicular fluid with neuraminidase and was inhibited by gangliosides. treatment of serum with proteases reduced aggregating activity. adsorption of serum to hydroxyapatite did not reduce the aggrega ... | 1983 | 6358038 |
| immunochemical comparison of cell-wall antigens of various viridans streptococci, including strain 2a2+3 hot from recurrent oral aphthous ulceration in man. | several studies suggest that patients with recurrent aphthous ulceration show cell-mediated and humoral immunity to antigens of streptococcus sanguis, particularly strain 2a2+3 hot which is said to be antigenically similar or identical to strep. sanguis strain atcc 10556. however, physiological classification as well as analysis of the immunologically dominant cell-wall antigens by immunoelectrophoresis and indirect immunofluorescence showed that the strain is actually a strain of streptococcus ... | 1983 | 6360105 |
| inactivation and stabilization of iga protease from the human oral bacterium streptococcus sanguis. | the oral bacterium, streptococcus sanguis, secretes an extracellular protease which specifically cleaves immunoglobulin a (iga). during in vitro growth of strep. sanguis at 37 degrees c the iga protease was completely inactivated within 2 h. lowering the growth temperature to 31 degrees c reduced the inactivation rate 50 per cent and a doubling of enzyme yield was obtained. the iga protease could be stabilized by the presence of whole human saliva, by a low concentration of iga, or by inhibition ... | 1983 | 6360106 |
| [immunoglobulin a protease secretion in streptococcus sanguis accelerated by actinomyces viscosus]. | 1983 | 6361182 | |
| protoplast and cytoplasmic membrane preparations from streptococcus sanguis and streptococcus mutans. | protoplasts were prepared from streptococcus sanguis and some s. mutans serotypes by use of lysozyme (ec 3.2.1.17) under particular conditions: cells had to be grown in dl-threonine (20 mm) and harvested in early exponential phase. the efficiency of protoplast formation was enhanced by two additional steps: plasmolysis (in 12% peg), prior to addition of lysozyme, and a swirling phase, after the enzymic action. this procedure allowed us to obtain clean protoplasts, with only 0.5% contamination by ... | 1983 | 6361217 |
| effect of insertion of osseo-integrated prosthesis on the oral microflora. | ten patients undergoing treatment with bridges supported by osseo-integrated oral titanium implants were studied regarding the oral microflora. saliva samples for microbiological studies were collected before the abutment operation, 1 week after the operation, after the bridges were inserted and again 52 weeks after the abutment operation. the implants did not induce colonization with "plaque streptococci" such as streptococcus mutans and streptococcus sanguis. a marked colonization with potenti ... | 1983 | 6362048 |
| peptide utilization by oral streptococci. | streptococcus mitis atcc 903 and streptococcus sanguis 804 could utilize large peptides present in proteose peptone as their sole source of nitrogen but grew poorly in amino acid medium. by contrast, streptococcus mutans bht and s. mutans ib grew in amino acid medium but could not utilize large peptides. streptococcus sp. strain bravato had requirements for both peptides and amino acids. peptide size did not seem important for the utilization of peptides by s. mitis except that dipeptides were n ... | 1984 | 6363292 |
| effect of saliva on coaggregation of oral actinomyces and streptococcus species. | human oral actinomyces and streptococci that exhibit specific coaggregation patterns when the cells are suspended in buffer were tested for their ability to coaggregate in saliva. of 53 paired combinations of actinomyces (actinomyces viscosus, a. naeslundii, or actinomyces sp. wva 963) and streptococci (streptococcus sanguis or s. morbillorum) that exhibited coaggregation in buffer, all but 4 pairs also coaggregated when suspended in saliva. twenty-four pairs exhibited lactose-inhibited coaggreg ... | 1984 | 6370861 |
| interference of alpha-hemolytic streptococci isolated from tonsillar surface on beta-hemolytic streptococci (streptococcus pyogenes)--a methodological study. | the interference between alpha-streptococcal strains obtained from patients with repeated tonsillitis and a collection of group a streptococcal strains were studied. for this purpose three in vitro methods were designed and compared. the results obtained by a simple plating technique suitable for screening purposes were found to correlate well with those using more laborious techniques. in a limited scale some of the alpha- and beta-streptococcal combinations were tested under in vivo conditions ... | 1983 | 6372328 |
| effect of volume of blood cultured on detection of streptococcus viridans bacteraemia. | fifty eight patients undergoing dental extraction each had 45 ml blood collected. this was divided into 30 ml and 15 ml blood samples for culture. the 30 ml sample was inoculated into 120 ml nutrient broth with 0.05% liquoid and the 15 ml sample into 60 ml of identical broth so that the final dilution of blood in broth was always 1/5. bacteraemia due to viridans streptococci was found in 27 and 15 patients by culturing the 30 ml and 15 ml blood samples respectively. only one further case of stre ... | 1984 | 6373833 |
| specific binding ability of the cells of streptococcus sanguis atcc 10557 to beta-d-galactopyranoside-bovine serum albumin conjugate. | 1983 | 6374077 | |
| detection of streptococcal mutants presumed to be defective in sugar catabolism. | the tetrazolium method for detection of bacterial mutants defective in sugar catabolism was modified for use with streptococci. the critical factors were (i) the concentration of tetrazolium, which must be titrated to determine the optimum concentration for each species or even strain, and (ii) anaerobic incubation of tetrazolium-containing agar plates. when used with standard mutagenesis protocols, this method yielded lactose-negative mutants of nine streptococcal strains representing six speci ... | 1984 | 6378096 |
| group a streptococcal peptidoglycan-polysaccharide inhibits phagocytic activity of human polymorphonuclear leukocytes. | injection of sterile aqueous preparations of the peptidoglycan-polysaccharide of group a streptococci (pg-aps) produces chronic inflammation in several animal models. chronic bacterial infection may be involved in some aspects of the pathogenesis of inflammation associated with the accumulation of pg-aps. accordingly, the effect of pg-aps on human neutrophil (polymorphonuclear leukocyte [pmn]) bactericidal activity was studied with the supposition that this interaction may contribute to the infl ... | 1984 | 6378796 |
| clinical, microbiological and immunological features associated with the treatment of active periodontosis lesions. | clinical, microbiological and immunological factors were examined using data from a subject with periodontosis. the subject was monitored at bimonthly intervals for 26 months at 6 sites per tooth for redness, plaque, suppuration, bleeding on probing, pocket depth, and attachment level. using attachment level measurements and the tolerance method of analysis, sites with active disease and control (inactive) sites of equal pocket depth were selected. subgingival plaque samples were taken from thes ... | 1984 | 6386896 |
| [electrical potential of metal prostheses and metal restorative materials in the oral cavity]. | 1983 | 6387301 | |
| the gene for type a streptococcal exotoxin (erythrogenic toxin) is located in bacteriophage t12. | the infection of streptococcus pyogenes t25(3) with the temperate bacteriophage t12 results in the conversion of the nontoxigenic strain to type a streptococcal exotoxin (erythrogenic toxin) production. although previous research has established that integration of the bacteriophage genome into the host chromosome is not essential for exotoxin production, the location of the gene on the bacteriophage or bacterial chromosome had not been determined. in the present investigation, recombinant dna t ... | 1984 | 6389348 |
| ability of various oral bacteria to bind human plasma fibronectin. | the present study describes the ability of various oral bacteria to bind human plasma fibronectin (pfn). avid binding of 125i-pfn was found for streptococcus mutans (serotypes a to h), streptococcus sanguis, group a streptococcus pyogenes and staphylococcus aureus, while other gram-positive bacterial species tested demonstrated only weak or negligible pfn binding ability. two gram-negative bacterial species, bacteroides gingivalis and escherichia coli, did not significantly bind pfn. 125i-pfn bi ... | 1984 | 6390098 |
| antibiotic susceptibility of streptococcal strains associated with infective endocarditis. | seventy-six strains of various species of streptococci isolated from patients with infective endocarditis were tested for their susceptibility to 13 antibiotics by an agar dilution method. the antibiotics tested were: benzyl-penicillin, ampicillin, cefotaxime, vancomycin, erythromycin, rifampicin, pristinamycin, gentamicin, netilmicin, tobramycin, amikacin, dibekacin and streptomycin. excluding enterococci, 91% of strains were sensitive to benzylpenicillin. resistance to benzylpenicillin was onl ... | 1984 | 6394332 |
| variable nature of the bacterial cell surface. | 1984 | 6399205 | |
| a complex attenuator regulates inducible resistance to macrolides, lincosamides, and streptogramin type b antibiotics in streptococcus sanguis. | macrolide-lincosamide-streptogramin b resistance specified by streptococcus sanguis plasmid pam77 involves an adenine methylase, whose synthesis, demonstrable both phenotypically and by analysis of methionine-labeled proteins made in bacillus subtilis minicells, is inducible by erythromycin, lincomycin, and streptogramin type b antibiotics. localization of the methylase structural gene, including its control region in dna fragments obtained with restriction endonucleases, has been deduced from d ... | 1983 | 6406429 |
| [characterization of the antigen in circulating immune complexes and application to the detection of streptococcal antigens in human infectious endocarditis]. | immune complexes from patients with subacute endocarditis were used to develop a methodology for characterization of the antigen involved in human circulating immune complexes. this model was chosen because it permits isolation of the causative agent of the streptococcal infection thought to contain the antigen present in the immune complex. a comparison was made between two methods for characterization of bacterial antigen bound to antibody. in the first, animals were immunized with purified im ... | 1983 | 6408976 |
| prevalence of viridans streptococci exhibiting lactose-inhibitable coaggregation with oral actinomycetes. | fresh oral isolates from human dental plaque were selected on the basis of their spherical morphology. in a double-blind experiment, their species identity and ability to coaggregate with human oral actinomyces viscosus and actinomyces naeslundii were determined. of the 110 isolates characterized, 30 were identified as either streptococcus mutans, streptococcus anginosus-constellatus, or veillonella parvula; none of these coaggregated with the actinomycetes. the remaining 80 isolates were identi ... | 1983 | 6409806 |
| new actinomyces and streptococcus coaggregation groups among human oral isolates from the same site. | the coaggregation properties of recent human oral streptococcal and actinomyces isolates from the same site were determined and compared with the coaggregation properties of well-characterized stock strains of these two kinds of bacteria. streptococcus sanguis, actinomyces viscosus, actinomyces naeslundii, and phenotypically similar strains of actinomyces were isolated from subgingival samples from periodontally healthy older individuals, from persons participating in an experimental gingivitis ... | 1983 | 6409807 |
| trimethylsilyl-sugar profiles of streptococcus milleri and streptococcus mitis. | seventy strains of 'viridans-group' streptococci were analysed gas chromatographically after preparation of trimethylsilyl ethers of their cellular sugars. the resulting profiles were evaluated as a possible aid to taxonomy. glycerol, glucose, galactose, n-acetyl-glucosamine and n-acetylmuramic acid were found in all strains, in varying amounts. rhamnose was the major neutral sugar in most strains, other than representatives of streptococcus mitis, which invariably had ribose and usually anhydro ... | 1983 | 6409876 |
| numerical taxonomy of streptococcus. | a numerical taxonomic study of strains of streptococcus, together with representatives of allied genera, showed 28 reasonably distinct phenons. the major areas, with their phenons, were: (a) enterococcal species group (s. faecalis, s. faecium, 's. avium' and a proposed new species 's. gallinarum'); (b) paraviridans species group (s. bovis, s. equinus, s. salivarius, 's. casseliflavus', s. mutans, s. raffinolactis and an unidentified oral group i); (c) lactic species group (s. lactis including s. ... | 1983 | 6409982 |
| streptococci isolated from the bloodstream and gingival crevice of man. | one hundred and twenty six strains of streptococci isolated from the bloodstreams of 123 hospital in-patients--55 with endocarditis--and 255 strains isolated from the gingival crevices of 66 volunteers were characterised. species isolated were streptococcus mitior, s. sanguis, undifferentiated viridans streptococci, s. salivarius, s. milleri, s. mutans, s. bovis and s. faecalis. there was no significant difference between the distribution of species in blood and in the gingival crevice. s. mitio ... | 1983 | 6410069 |
| purification and immunochemical characterization of streptococcus sanguis atcc 10557 serotype ii carbohydrate antigen. | cell wall carbohydrate antigen of streptococcus sanguis atcc 10557 (serotype ii/biotype b) was extracted from purified cell walls by treatment with 5% trichloroacetic acid at 4 degrees c for 8 h. the extract was purified by chromatography on deae-sephadex a-25 and sephadex g-100 columns. the purified carbohydrate antigen produced a single precipitin band against anti-type ii serum, which fused with the band produced by the autoclaved extract or the phenol-water extract of the s. sanguis cells. t ... | 1983 | 6417021 |
| inhibition of microbial iga proteases by human secretory iga and serum. | microbial iga proteases cleave human serum iga1 immunoglobulin, but human secretory iga is resistant to hydrolysis. we have found this resistance to be due to an inhibition of protease activity that is mediated by the fab region of secretory iga. the iga proteases of the genus neisseria are more sensitive to inhibition than is the protease of streptococcus sanguis. there is also a serum inhibitor of neisseria proteases that co-chromatographs with igg. monoclonal (myeloma) human igg proteins and ... | 1983 | 6417473 |
| competent antigen-binding fragments (fab) from secretory immunoglobulin a using streptococcus sanguis immunoglobulin a protease. | 1984 | 6423284 | |
| inhibition of neisseria gonorrhoeae growth due to hydrogen peroxide production by urogenital streptococci. | seven streptococci isolated from the normal urogenital flora were selected for the production of an inhibitory activity toward neisseria gonorrhoeae on solid medium. this activity was abolished when catalase was added to the medium in the case of streptococcus mitis isolates 22, 25, 74 and streptococcus sanguis ii isolate 70 suggesting the involvement of hydrogen peroxide. the inhibitory activities produced by the other isolates (streptococcus faecalis) were trypsin sensitive (isolates 12 and 14 ... | 1984 | 6427555 |
| carbohydrate composition of dental plaque from primates with irradiation caries. | carbohydrate analyses were performed on dental plaque collected from the teeth of irradiated monkeys, non-irradiated monkeys and a group of streptococcus mutans free animals, all of which were fed the same standard cariogenic diet. glucose was the predominant sugar constituent in plaque and was detected in highest concentration in the irradiated animals. small amounts of pentoses and other hexoses were also present. plaque from irradiated animals contained, by comparison with the other groups, h ... | 1984 | 6429296 |
| transformation of streptococcus sanguis challis with streptococcus lactis plasmid dna. | streptococcus lactis plasmid dna, which is required for the fermentation of lactose (plasmid plm2001), and a potential streptococcal cloning vector plasmid (pdb101) which confers resistance to erythromycin were evaluated by transformation into streptococcus sanguis challis. plasmid plm2001 transformed lactose-negative (lac-) mutants of s. sanguis with high efficiency and was capable of conferring lactose-metabolizing ability to a mutant deficient in enzyme iilac, factor iiilac, and phospho-beta- ... | 1984 | 6435522 |
| identification of streptococci in a medical laboratory. | a total of 965 cultures of streptococci received at a reference unit for identification were examined with api-20 strep kits and also by established methods. the api method, although it needed to be supplemented with additional tests, largely overcame the difficulty that pyogenic streptococci are usually identified by their serological reactions and that biochemical tests are used for the identification of the other streptococci. representatives of at least 24 established or possible species wer ... | 1984 | 6436221 |
| [conjugational plasmid transfer from a, b and h streptococci to n streptococci]. | plasmid-mediated resistance to erythromycin and chloramphenicol was successfully transferred from group a, b and h streptococci to group n streptococci by a process akin to conjugation. the results showed that plasmids from streptococcal groups other than n were able to replicate in lactic streptococci as well. the transfer experiments were carried out by using a membrane filter mating technique. four of the five plasmids used (psm15346, psm10419, pip501, and pel1) were transferred at frequencie ... | 1984 | 6441362 |
| inhibition of bacterial growth by tetracycline-impregnated enamel and dentin. | tetracyclines can react with enamel and dentin to form relatively insoluble fluorescent compounds. the purpose of this study was to evaluate the possible antimicrobial effect of these reaction products on various microorganisms associated with human dental plaque and periodontal disease. slabs of native dentin and enamel as well as demineralized dentin were immersed in aqueous solutions of tetracycline hcl, oxytetracycline hcl and doxycycline hcl for periods of 1 h or 24 h. unimpregnated enamel ... | 1984 | 6441999 |
| interaction of inflammatory cells and oral microorganisms. ix. the bactericidal effects of human polymorphonuclear leukocytes on isolated plaque microorganisms. | 1980 | 6449581 | |
| glucose and sucrose effects in the culture media on streptococcus sanguis omz 9 glucosyltransferase inhibition by anti-glucosyltransferase antibodies. | an anti-glucosyltransferase serum was prepared against a purified enzyme of streptococcus sanguis omz 9. antibody fractions obtained after gel filtration on a bio-gel p200 column were used to study enzyme-antibody interaction in broth culture containing either sucrose or glucose, and to localize ultrastructurally the enzyme on thin sections of bacteria using labelled and unlabelled antibodies. the antibody fractions had no effect on bacterial growth in broth culture. a strong inhibition of insol ... | 1980 | 6451617 |
| [discussion on the etiology of ulcerative colitis (uc)]. | 1980 | 6451867 | |
| adherence of streptococcus sanguis clinical isolates to smooth surfaces and interactions of the isolates with streptococcus mutans glucosyltransferase. | streptococcus sanguis isolated from human dental plaque were grown in todd-hewitt broth. cells were collected by centrifugation and lyophilized after extensive washing with water. the cell-associated glucosyltransferase (gtase) activities of s. sanguis strains were assayed with [14c]sucrose. strain differences in gtase activity were significant within the same serotype or biotype or both. the ability of s. sanguis cells to adhere to smooth glass surfaces was generally weak, irrespective of signi ... | 1981 | 6452415 |
| [extracellular polysaccharides of plaque streptococci. 1. thin-layer chromatographic studies (author's transl)]. | 1981 | 6455883 | |
| inactivation of d-glucosyltransferases from oral streptococcus mutans and streptococcus sanguis by photochemical oxidation. | cell-free d-glucosyltransferase of d-glucose-grown streptococcus mutans aht was completely inactivated in the presence of 0.002% of methylene blue at 25 degrees and ph 7.0 after illumination with a 150-w incandescent lamp. the rate of inactivation was decreased at ph values less than 7.0. histidine was the only amino acid residue modified to a significant extent, and the rates of oxidation of histidine residues and loss of enzyme activity closely agreed. production of both water-insoluble and -s ... | 1981 | 6456065 |
| effects of local immunization of hamsters with glucosyltransferase antigens from streptococcus sanguis on dental caries caused by streptococcus mutans. | 1981 | 6461318 | |
| lactose transport in streptococcus mutans: isolation and characterization of factor iiilac, a specific protein component of the phosphoenolpyruvate-lactose phosphotransferase system. | the transport of lactose in streptococcus mutans is mediated via an inducible phosphoenolpyruvate-lactose phosphotransferase system. this system requires for catalytic activity a membrane fraction (enzyme ii), two general proteins called enzyme i and hpr, and a soluble specific protein termed factor iiilac. this protein factor was purified from s. mutans atcc 27352 by chromatographies on deae-cellulose, hydroxylapatite, ultrogel aca 34, and phosphocellulose. the purified protein migrated as a si ... | 1984 | 6480107 |
| cell surface proteins of oral streptococci. | whole cells of representative strains of oral streptococci (streptococcus sanguis, s. mitis, and s. salivarius) were radiolabeled by the lactoperoxidase method of radioiodination. the labeled polypeptides obtained by extraction of whole cells with boiling sodium dodecyl sulfate were analyzed by polyacrylamide gel electrophoresis and autoradiography. of the total radioactivity, ca. 70% was released by treating whole cells with trypsin, suggesting that the labeling was confined to proteins located ... | 1984 | 6480109 |
| a possible mechanism for the cellular coaggregation between actinomyces viscosus atcc 19246 and streptococcus sanguis atcc 10557. | the cells of actinomyces viscosus atcc 19246 (av19246) and streptococcus sanguis atcc 10557 (ss10557) coaggregated immediately after mixing in 40 mm-tris/hcl buffer. optimal conditions were ph 7.5 in the presence of ca2+ at 0.1 mm or higher. na2 edta and its analogues, na2mgedta and na2mnedta at 7.5 mm inhibited the coaggregation. trypsin and heat treatment impaired the reactive site on av19246 cells, but not on ss10557 cells. the coaggregates, once formed, dissociated gradually during extended ... | 1984 | 6481336 |
| spinal epidural abscess: an unusual complication of bacterial endocarditis. | we are reporting a case of epidural abscess complicating bacterial endocarditis. to the best of our knowledge, this association has not been reported before. streptococcus sanguis was isolated from the blood. a full recovery followed surgery and antibiotic therapy. the relevant characteristics of both diseases are reviewed. no explanation for this rare association is available as yet. | 1984 | 6490169 |
| longitudinal investigation of bacteriology of human fissure decay: epidemiological studies in molars shortly after eruption. | in the present investigation, the proportions of streptococcus mutans, lactobacilli, streptococcus sanguis, veillonellae, and an unidentified actinomyces-like organism in dental plaque on occlusal fissures of first mandibular molars were monitored at 6-month intervals over a 3-year period in 368 children who were initially in grades 1 or 2. teeth destined to become decayed exhibited a significant increase in the proportions of s. mutans from 6 to 24 months before the diagnosis of dental decay. l ... | 1984 | 6500709 |
| genetic exchange between oral streptococci during mixed growth. | to determine whether oral streptococci might exchange genetic information in the oral cavity, paired transformable strains of streptococcus mutans, streptococcus sanguis and streptococcus milleri were growth together. chromosomal and plasmid-borne antibiotic resistance markers could be readily transferred from s. mutans gs-5 to s. milleri nctc 10707 or s. sanguis challis during mixed growth. however, no exchange from the latter two organisms to strain gs-5 could be detected under these condition ... | 1984 | 6512502 |
| viridans streptococci in infective endocarditis: species distribution and susceptibility to antibiotics. | a method for the speciation of viridans streptococci (non-groupable) is described. the major identification criteria are based on the reactions to a series of biochemical tests, including acid production in lactose, inulin, raffinose, mannitol and sorbitol, hydrolysis of arginine, esculin and na hippurate, and production of polysaccharides in 5% sucrose media. a total of 450 strains was isolated from blood cultures, 183 of which were from confirmed cases of subacute endocarditis. the latter were ... | 1984 | 6519085 |
| [hydrolysis of streptococcal polysaccharides by micromycete dextranases]. | the effect of dextranase enzyme preparations obtained from penicillium piscarium bim g-102, penicillium funiculosum, aspergillus insuetus g-116 and aspergillus ustus on polysaccharides synthesized by cariesogenic streptococcus sanguis and streptococcus mitis was being studied. according to the data obtained dextranases from p. piscarium, p. funiculosum and asp. ustus can be considered as a promising anticarious agent. | 1984 | 6540443 |
| resistance in oral streptococci after repeated two-dose amoxycillin prophylaxis. | twelve normal volunteers received two doses of 3 g amoxycillin at weekly intervals on up to five occasions. amoxycillin-resistant oral streptococci were not isolated from any subject beforehand, but they had appeared in all eleven subjects (who could be included in the analysis) by the end of the investigation, and in one subject after one administration of double dose amoxycillin. resistant streptococci were undetectable in all volunteers 13 weeks after their last dose of amoxycillin. all resis ... | 1983 | 6555190 |
| the antibacterial effects of calcium hydroxide apexification pastes on streptococcus sanguis. | four calcium hydroxide-based apexification pastes were tested for their antibacterial effects on streptococcus sanguis. their zones of growth inhibition on blood agar plates were measured at 2, 4, 6, and 8 days. only the camphorated parachlorophenol and the metacresylacetate pastes showed zones of inhibition. both of these zones of inhibition decreased with time; however, the zones of inhibition for the parachlorophenol paste decreased at a slower rate. | 1983 | 6572354 |
| purification and characterization of galactosephilic component present on the cell surfaces of streptococcus sanguis atcc 10557. | previous studies have indicated that a galactosephilic component present on the bacterial cell surfaces of streptococcus sanguis atcc 10557 may be responsible for the salivary glycoprotein-mediated binding of the cells. the purpose of this study was to investigate the purification and characterization of galactosephilic cell surface component from s. sanguis atcc 10557. a galactosephilic component involving fibrils on the cell surfaces was isolated by the techniques of freezing and thawing, and ... | 1983 | 6573474 |
| effect of snf2, administered as mouthrinses or topically applied, on streptococcus mutans, streptococcus sanguis and lactobacilli in dental plaque and saliva. | mouthrinsing with snf2 reduced the streptococcus mutans population in plaque and saliva and the proportion of streptococcus sanguis in plaque. the effect was of short duration: 2 weeks after treatment the values of s. mutans in plaque and saliva were even higher than the pretreatment values. topical snf2 applications reduced the s. mutants population in plaque and saliva but did not reduce the proportion of s. sanguis in plaque. the effect was more prolonged : 4 weeks after treatment the s. muta ... | 1983 | 6574579 |
| inhibition by the antimicrobial agent chlorhexidine of acid production and sugar transport in oral streptococcal bacteria. | oral streptococci transport sugars via the phosphoenolpyruvate-phosphotransferase (pep-pts) system. in a specific assay of this system, low concentrations of chlorhexidine abolished the activity of the glucose and sucrose pts in batch-grown cells of streptococcus mutans ingbritt and b13, strep. sanguis nctc 7865, strep. mitis atcc 903, strep. milleri nctc 10709 and strep. salivarius nctc 8606. intact cells and cells made permeable to the assay reagents with toluene were used. toluenized cells we ... | 1983 | 6574734 |
| control of sugar utilization in the oral bacteria streptococcus salivarius and streptococcus sanguis by the phosphoenolpyruvate: glucose phosphotransferase system. | three different strep. salivarius (g2, g5 and g29) and two strep. sanguis (gs3 and gs12) mutants affected in the phosphoenolpyruvate: glucose phosphotransferase system were selected on agar plates containing lactose and 2-deoxyglucose. all 5 were defective in a membrane-bound component of the transport system and grew less rapidly than the parent strain in 5 mm glucose-containing medium. mutants g2 and g29 grew poorly in the presence of 5 mm mannose. growth on mixed substrates revealed that the ... | 1983 | 6575744 |
| comparative growth responses of oral streptococci on mixed saliva or the separate submandibular and parotid secretions from caries-active and caries-free individuals. | growth of s. mutans on mixed or parotid saliva from cf individuals may be influenced by the availability of growth-supportive proteins or the inhibitory activity present in parotid saliva. a deficiency in growth-supportive proteins may explain the limited growth of s. sanguis on mixed or submandibular saliva from these individuals. | 1983 | 6575997 |
| prophylaxis of experimental endocarditis in rabbits using one or two doses of amoxycillin. | 1983 | 6577902 | |
| the survivability of f43 strain of streptococcus sanguis in root canals filled with gutta-percha and procosol cement. | 1983 | 6579164 | |
| [purification and characterization of the lectin present on the cell surfaces of streptococcus sanguis atcc 10557]. | 1983 | 6579272 | |
| association of food lectins with human oral epithelial cells in vivo. | the association of wheat germ agglutinin (wga) and peanut agglutinin (pna) with oral tissues after eating raw wheat-germ or raw peanuts, respectively, was determined. an indirect enzyme-linked immunoassay (elia) was used to detect wga and pna on epithelial cells. buccal and tongue epithelial cells, and preparations of salivary sediment exhibited elia units of wga and pna significantly above background after eating 2--7 g of wheat-germ or 25 g of raw peanuts, respectively. both lectins were detec ... | 1983 | 6579890 |
| predominant cultivable microflora of plaque on removable dentures in patients with healthy oral mucosa. | plaque from the fitting surface of upper full dentures in eight patients with healthy palatal mucosa was studied. to characterize the predominant cultivable flora, 916 isolates (100-128 from each sample) were subcultured from anaerobic roll-tubes. streptococci constituted 0-81 per cent (median, 41 per cent) of the isolates with varying proportions of streptococcus milleri, streptoccus mutans, streptococcus salivarius, streptococcus mitior and streptococcus sanguis. staphylococcus aureus made up ... | 1983 | 6579900 |
| implantation of transformant strains of the bacterium streptococcus sanguis into adult human mouths. | streptococcus sanguis strains isolated from dental plaque of 12 subjects were screened for their ability to undergo genetic transformation using a streptomycin-resistance marker. all subjects harboured at least one transformable strain. eight of the subjects were implanted with transformant strains originally isolated from their own mouth, whereas four subjects received bacteria from other donors. the strep. sanguis transformants became successfully implanted; their oral levels remained virtuall ... | 1983 | 6579905 |
| stability of streptococcus mutans and its relationship to caries in a child population over 2 years. | 1983 | 6580079 | |
| heterofermentative glucose metabolism by glucose transport-impaired mutants of oral streptococcal bacteria during growth in batch culture. | spontaneous mutants defective in a membrane component of the phosphoenolpyruvate-glucose phosphotransferase system were isolated by plating cells of streptococcus sanguis 10556, streptococcus mutans gs5-2 and nctc 10449 on agar containing lactose and 2-deoxyglucose. toluenized cells of these mutants were defective in their ability to catalyse the phosphoenolpyruvate-dependent phosphorylation of 2-deoxyglucose. the parental strains were mainly homofermentative when grown in batch culture in the p ... | 1983 | 6580849 |
| [bacterial flora in dental intercoronal fissures based on a literature review]. | 1983 | 6581900 | |
| neuraminidase activity: a biochemical marker to distinguish streptococcus mitis from streptococcus sanguis. | selected reference and freshly isolated strains of streptococcus mitis (mitior) and streptococcus sanguis were assayed for cell-associated neuraminidase activity by their ability to hydrolyze [3h-] sialyllactitol. a cell-associated neuraminidase was detected with s. mitis and s. sanguis serotype ii (reclassified as s. mitis) but not with s. sanguis serotypes i and iii. neuraminidase activity of s. mitis correlated with this organism's inability to hydrolyze arginine, aesculin, and few, if any, s ... | 1984 | 6582090 |
| a conceptual model for the co-existence of streptococcus spp. and actinomyces spp. in dental plaque. | one of the most important questions in ecology is how to explain the co-existence of the variety of physiologically related organisms in the same habitat. a model is presented for the co-existence of streptococcus species and actinomyces species in dental plaque. the hypothesis is that these organisms co-exist because they simultaneously utilize several carbon and energy substrates. the hypothesis follows from the observation that the growth yield of oral streptococci and actinomyces in saliva i ... | 1984 | 6583241 |
| colonization of the subgingival area by bacteroides gingivalis. | the initial event in colonization of the subgingival area by b. gingivalis is its attachment to host cells and gram-positive bacteria in pre-formed plaque. the level of b. gingivalis is partly governed by products of other plaque bacteria, especially by sanguicin. once b. gingivalis resides in its nidus and starts to proliferate, expulsion of pre-existing residents may occur, especially of attached gram-positive bacteria, through the inhibitory action of the b. gingivalis product, hematin. the b ... | 1984 | 6583244 |
| inhibition of lactose-reversible adherence between actinomyces viscosus and oral streptococci by salivary components. | 1984 | 6584210 | |
| factors involved in artificial caries induction by oral streptococci in extracted human teeth. | this study assesses the abilities of s. mutans gs5 and bht and s. sanguis g9b to produce subsurface lesions on smooth surfaces of extracted human teeth and examines factors which might be responsible for any differences encountered. teeth were incubated in brain heart infusion broth containing 2% sucrose and a pure culture of the organism to be tested, the media being changed each day for eight days. surface and media ph's were measured. the mineral content of both the surface enamel and the sub ... | 1984 | 6584469 |
| an investigation of the effects of maltose and sucrose in the diet on the microbiology of dental plaque in man. | in a double-blind cross-over study, 24 dental students consumed 150 g of sucrose or maltose a day in various foods. after 15 days, plaque samples were collected from the distal surface of upper right first premolars; post-diet samples were collected four months later. the median total anaerobic count of plaque exposed to sucrose was significantly lower compared with maltose samples (p less than 0.05). glucan-producing organisms per 100 anaerobes in the sucrose samples compared with the maltose ( ... | 1984 | 6586127 |
| plaque formation on the hydrophobic composite resin. | 1983 | 6586351 | |
| [studies on materials for root caries restoration--adherence of plaque bacteria on the materials' surface]. | 1983 | 6586887 | |
| exohemagglutinin isolated from the cultured medium of streptococcus sanguis atcc 10557. | 1983 | 6587050 | |
| streptococcus mutans and streptococcus sanguis in plaque from orthodontic bands and brackets. | 1984 | 6587970 | |
| microbiological assessment of dental plaque on bovine enamel implants worn intra-orally in children. | nine caries-active children, each from six to nine years old, wore small removable enamel implants intra-orally for four weeks as part of an effort to develop removable model systems for studying dental plaque in fissures. the implants, consisting of two enamel cubes arranged to simulate a fissural space, were cemented in box preparations in stainless steel crowns on pulpotomized primary molars. upon removal, the fissural spaces were opened to facilitate plaque harvesting. the plaque samples wer ... | 1984 | 6588083 |
| [distribution of streptococcus, s. mutans and s. sanguis in the human oral cavity]. | 1983 | 6589057 | |
| [bacteria as an etiological agent of human dental caries]. | 1984 | 6589328 | |
| [a periodontologic study of bacteria in the periodontal pocket--in vitro adherence of bacteria to the metal materials of periodontal probes]. | 1984 | 6590694 | |
| [coaggregation reactions between oral bacteria]. | 1984 | 6591474 | |
| effect of chlorhexidine and iodine on the composition of the human dental plaque flora. | 1984 | 6592041 | |
| effects of chlorhexidine, iodine, and 5,7-dichloro-8-hydroxyquinoline on the bacterial composition of rat plaque in vivo. | 1984 | 6592046 |