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from micrococcus to moraxella. the reemergence of branhamella catarrhalis. 19902122824
[antibacterial properties of urine and the clinical course of pyelonephritis in patients with multiple myeloma]. 19902128136
production of a bacteriophage, a phage tail-like bacteriocin and an antibiotic by bacillus azotofixans.twenty-two nitrogen-fixing bacillus azotofixans strains were shown to produce an inhibition zone against themselves in plate assays. the b. azotofixans type strain p3l-5, chosen for further studies, produced inhibition zones against various bacillus strains and other bacterial genera. this antibacterial substance was also produced in liquid medium and its production was enhanced in semisolid medium (0.4% agar) after 3 to 5 days of incubation. the substance was suggested to be an antibiotic and i ...19902129100
isolation and characterization of bacteria-induced protein p4 from hemolymph of manduca sexta.insects synthesize several types of hemolymph proteins in response to bacterial infection. the objective of this study was to characterize a 48,000 dalton hemolymph protein induced in larvae of manduca sexta after injection of bacteria. the protein, isolated by cation exchange and gel filtration chromatography from hemolymph of larvae injected with micrococcus lysodeikticus, was found to be a glycoprotein with pi = 8.4. the molecular weight, isoelectric point, amino acid composition, and nh2 ter ...19902134020
[studies of antigens related to metabolic and growth inhibitions of staphylococcus aureus l-form].metabolic and growth inhibiting activities in immunoglobulin (of anti-s. aureus l-form serum and anti-s. aureus coccal form serum) could be absorbed by cell membranes of s. aureus l-form and its coccal form, respectively. these activities could not be absorbed by cell membrane of micrococcus luteus, streptococcus pyogenes or actinomyces viscosus. these findings suggested the existence of species-specific antigens of cell membrane. the membrane antigens of l-form related to the metabolic and grow ...19902135412
an electron paramagnetic resonance probe to detect local z-dna conformations.we present spectroscopic evidence for an electron paramagnetic resonance (epr) probe to detect local z-dna conformations in synthetic dna. a spin labeled deoxycytidine-5'-triphosphate (pppdcat) was co-polymerized with micrococcus luteus dna polymerase to yield the spin active alternating co-polymer (dg-dc,dcat)n. the epr spectrum of (dg-dc,dcat)n in the z-dna conformation indicates a decrease in the local base dynamics by about a factor of two as compared to that computed for b-dna. a control ex ...19902154981
the distribution of electron flow in the branched respiratory chain of micrococcus luteus.endogenous coupled respiration of micrococcus luteus protoplasts showed a relatively high resistance to low concentrations of kcn, 2-nonyl-4-hydroxyquinoline n-oxide (nqno) and dicyclohexylcarbodi-imide (dccd) when the inhibitors were applied individually. in the presence of both kcn and nqno (or dccd), o2 uptake was strongly inhibited. the proteolysis of external membrane proteins of protoplasts also induced the high sensitivity of endogenous coupled respiration to low kcn. the effects of nqno, ...19902156496
archaebacterial reverse gyrase cleavage-site specificity is similar to that of eubacterial dna topoisomerases i.atp-dependent type i topoisomerases from extremely thermophilic archaebacteria--reverse gyrases--drive positive supercoiling of dna. we showed that reverse gyrase from desulfurococcus amylolyticus breaks the dna at specific sites and covalently binds to the 5' end. in 30 out of 31 sites located in pbr322 dna fragments, cleavage occurs at the sequence 5'---cnnn/---(n is any base). the same rule was previously shown to hold for single-stranded dna breakage by eubacterial topoisomerases i. the rela ...19902160070
mcri: a novel class-ii restriction endonuclease from micrococcus cryophilus recognizing 5'-cgry/cg-3'.a new class-ii restriction endonuclease, mcri, with a novel sequence specificity as isolated from the gram-positive eubacterium micrococcus cryophilus. mcri recognizes the palindromic hexanucleotide sequence. [sequence: see text] the novel enzyme in the presence of mg2(+)-ions cleaves specifically both strands as indicated by the arrows. the staggered cuts generate 3'-protruding ends with single-stranded 5'-ry-3' dinucleotide extensions. the mcri recognition sequence was deduced from mapping dat ...19902162784
biosynthesis of benzylpenicillin (g), phenoxymethylpenicillin (v) and octanoylpenicillin (k) from glutathione s-derivatives."in vitro" synthesis of benzylpenicillin and phenoxymethylpenicillin has been carried out by direct n-acylation of 6-aminopenicillanic acid (6-apa) with s-phenylacetyl- and (s-phenoxyacetyl)glutathione. the reactions were catalyzed by the enzyme acyl-coa: 6-apa acyltransferase (at) from penicillium chrysogenum and in both cases the synthesis of antibiotics was enhanced by coa. penicillin k, a natural penicillin, was also synthesized "in vitro" by incubating (s-octanoyl)glutathione, 6-apa and at, ...19902166024
in vitro inhibition of bacterial dna gyrase by cinodine, a glycocinnamoylspermidine antibiotic.cinodine, a broad-spectrum glycocinnamoylspermidine antibiotic, binds to dna and irreversibly inhibits bacterial and phase dna synthesis. cinodine was found to inhibit the activity of micrococcus luteus dna gyrase in vitro, but it did not inhibit the activities of two other dna-binding enzymes, namely, topoisomerase i and bamhi. although we cannot yet conclude that dna gyrase is an intracellular target of the drug, in vitro inhibition of the enzyme by cinodine appears to be specific.19902167040
structure of the dnaa region of micrococcus luteus: conservation and variations among eubacteria.a phylogenetic tree constructed by 5s rrna analysis is composed of three major branches in eubacteria: high g + c gram+, low g + c gram+ and gram- [hori and osawa, mol. biol. evol. 4 (1987) 445-472]. we have shown that the characteristic dnaa region is common among escherichia coli (gram-), pseudomonas putida (gram-), and bacillus subtilis (low g + c gram+). we have now determined the structure of the dnaa region of micrococcus luteus, as a representative of the last branch, high g + c gram+. th ...19902172090
radical-mediated dna damage in presence of oxygen. 19902172712
base dynamics of local z-dna conformations as detected by electron paramagnetic resonance with spin-labeled deoxycytidine analogues.conformation detection and base dynamics of spin-labeled z-dna have been investigated by electron paramagnetic resonance (epr) spectroscopy. the two synthesized and characterized probes used in this study were c(5)-nitroxide-labeled 2'-deoxycytidine 5'-triphosphates, pppdcat and pppdcavat, which serve as suitable substrates for micrococcus luteus dna polymerase. enzymatic incorporation of these probes into (dg-dc)n yields the epr-active alternating copolymers (dg-dc,dcat)n and (dg-dc,-dcavat)n. ...19902176827
aliphatic molecules (c-6 to c-8) containing double or triple bonds as potential penicillin side-chain precursors.three different hexenoyl-coa derivatives (trans-2-hexenoyl-coa, trans-3-hexenoyl-coa and trans-trans-2,4-hexadienoyl-coa), two octenoyl-coa (trans-2-octenoyl-coa, trans-3-octenoyl-coa) and 2-octynoyl-coa were tested as substrates of the enzyme acyl-coa: 6-aminopenicillanic acid acyltransferase (at) from penicillium chrysogenum. only trans-3-hexenoyl-coa and trans-3-octenoyl-coa were recognized by at and efficiently converted into penicillin f and octenoylpenicillin, respectively. the km values f ...19902177462
[the breakdown of phthalic acid esters by microorganisms].the distribution in environment of phthalic acid esters classified according to their inherent toxicity, teratogenic, mutagenic properties and cancerogenic activity into a group of priority pollutants of biosphere is described in this review. the role of microbial community in degradation of phthalate esters has been demonstrated. the effect of such environmental factors as ph of the medium, temperature, humidity, concentration of organic matter, the introduction of promising microbial strains i ...19902189501
cation-dependent solubilization of rat thymocyte chromatin is closely related to decondensation of the nuclei.the cation-dependent solubilization of rat thymocyte chromatin has been compared with decondensation of the nuclei as a function of sodium phosphate-mediated changes in the concentration of mg2+ and na+. after digestion of the nuclei with dnase i or micrococcus nuclease for a time just sufficient to permit extraction of a maximal amount of chromatin (minimum digestion), solubilization of most of the chromatin was found to occur with the same cation dependency as decondensation of untreated nucle ...19902223879
external eye flora as a nutrient source for acanthamoeba.certain bacteria cause excystment of acanthamoeba from cyst to trophozoite form and are then ingested by migrating trophozoites. we studied the response of acanthamoeba cysts to inoculation on agar seeded with three types of commensal eye bacteria and escherichia coli. amoebae excysted on all bacteria tested, and the migration rate of acanthamoeba trophozoites on each was compared. acanthamoeba migrated with equal speed on e. coli and staphylococcus epidermidis. migration was observed, but was m ...19902227492
characterization of sodium dodecyl sulfate-stable staphylococcus aureus bacteriolytic enzymes by polyacrylamide gel electrophoresis.profiles of the bacteriolytic activities of staphylococcus aureus culture supernatants, sodium dodecyl sulfate cell extracts, licl cell extracts, cell wall extracts, and cell membranes were analyzed in sodium dodecyl sulfate-polyacrylamide gels containing micrococcus luteus or s. aureus. a total of 20 distinct bands of bacteriolytic activity could be detected in gels containing m. luteus, 8 of these bands were found in culture supernatants. the sodium dodecyl sulfate cell extracts, the licl cell ...19902228971
[bacterial flora in the urethra of men with chlamydia trachomatis urethritis before and after treatment with ofloxacin].from urethra of 40 men with ctu, 190 bacterial strains were isolated, 110 before and 80 after treatment with ofx (400 mg daily for 5 days). among the strains coexisting with chlamydia trachomatis, staphylococcus epidermidis (30.9%), micrococcus (13.6%), streptococcus group d (10.9%) and staphylococcus aureus (10.9%) were isolated more frequently than others. the in vitro susceptibility testing showed that, in comparison to erythromycin, doxycycline, oxytetracycline and co-trimoxazole, ofx was th ...19902244013
micrococcus endophthalmitis. 19902244829
residues of macrolide antibiotics in eggs following medication of laying hens.1. the elimination kinetics of four macrolide antibiotics (tylosin, erythromycin, spiramycin and josamycin) in eggs were determined separately for albumen and yolk after oral administration through either drinking water or diet or after intramuscular injection. 2. residues were assayed by a plate diffusion technique in cylinders with micrococcus luteus as the test-organism. 3. drug excretion was usually over a longer time in the yolk. spiramycin was the most highly excreted in the egg whereas se ...19902245358
[micrococci in the skin microbiocenosis of the breasts. ecologic characteristics of the colony].the ecological characteristics of the micrococcal community on the skin of the mammary glands of nulligravidae, pregnant and puerperant women have been studied. the species composition of the community, the population size and occurrence of each species, its participation in the dominant structure of the community have been determined. the comparative evaluation of its types has been made. a decrease in the dominating role of m. luteus in pregnant and puerperant women has been established. the t ...19902256397
bacterial production of histamine in some tropical fish.quantitative and qualitative distribution of histamine-forming bacteria associated with the fish rastrelliger kanagurta, sardinella longiceps, sillago sihama and liza subviridis, were investigated. these bacteria constituted a significant portion of the total bacterial population of fish and the values obtained in the present study were higher than those previously reported. the order of quantitative abundance of histamine-forming bacteria in the fish examined was: s. longiceps greater than r. k ...19902259288
[comparative characteristics of lysozymes of different origin].lysozymes with different molecular weights were isolated from homogenates of ticks or ixodoidea with a procedure based on specific sorption of the enzyme by chitin. lysozymes with a molecular weight of 13,800 were isolated from o. moubata, o. papillipes and a. lahorensis and lysozymes with a molecular weight of 15,000 were isolated from h. asiaticum and i. persulcatus. micrococci and staphylococci proved to be the most sensitive to the lysozymes. e. coli and salmonella spp. were less sensitive. ...19902264747
identification and catabolic activity of well-derived gasoline-degrading bacteria from a contaminated aquifer.approximately 300 gasoline-degrading bacteria were isolated from well water and core material from a shallow coastal aquifer contaminated with unleaded gasoline. identification of 244 isolates revealed four genera: pseudomonas, alcaligenes, nocardia, and micrococcus, with pseudomonads making up 86.9% of bacteria identified. a total of 297 isolates was sorted into 111 catabolic groups on the basis of aerobic growth responses on 15 gasoline hydrocarbons. each test hydrocarbon was degraded by at le ...19902268163
phosphorescence and optically detected magnetic resonance studies of echinomycin-dna complexes.echinomycin complexes with polymeric dnas and model duplex oligonucleotides have been studied by low-temperature phosphorescence and optical detection of triplet-state magnetic resonance (odmr) spectroscopy, with the quinoxaline chromophores of the drug used as intrinsic probes. although not optically resolved, plots of odmr transition frequencies versus monitored wavelength revealed heterogeneity in the phosphorescence emission of echinomycin, which was ascribed to the presence of two distinct ...19902271577
antibacterial activity in the hemolymph of myriapods (arthropoda).the hemolymphs of two diplopod (chicobolus sp. and rhapidostreptus virgator) and two chilopod species (lithobius forficatus and scolopendra cingulata) were tested for the presence of antibacterial substances using petri dish tests. the native hemolymph of all species had substances acting on living micrococcus luteus, whereas only rhapidostreptus, scolopendra, and lithobius were effective against lyophilized micrococcus. the antibacterial activity against living micrococcus increased after inocu ...19902273286
the nucleotide sequence of an alpha-amylase gene from an alkalopsychrotrophic micrococcus sp.an alpha-amylase gene from micrococcus sp. 207 was cloned into escherichia coli jm101 using the vector phsg399. the constructed recombinant plasmid pyk63 contained a 4.8 kb chromosomal dna fragment derived from strain 207 dna. the cloned amylase isolated from e. coli jm101 (pyk63) produced mainly maltotetraose from starch, and exhibited temperature and ph activity profiles closely similar to those of the enzyme from the original strain. nucleotide sequence analysis of the cloned dna fragment rev ...19902276614
stomatococcus mucilaginosus bacteremias. typical case presentations, simplified diagnostic criteria, and a literature review.even though stomatococcus mucilaginosus is considered indigenous oral-pharyngeal flora, cited literature and case reports indicate that it can be the cause of infectious conditions. tested strains were isolated from blood, the oral region, and wound sources. the organism was routinely misidentified or not identified by conventional or commercial systems (vitek, staph-trac). four antimicrobial diagnostic disks for example, bacitracin (0.04 units; taxo a), furazolidone (100 micrograms), novobiocin ...19902279383
comparison of two kinds of cell packets of micrococcus luteus by scanning electron microscopy: outermost layer maintaining the packet structure.two kinds of cell packets of micrococcus luteus, one having teichuronic acids (tua) in the cell wall and the other lacking tua, have been independently reported by two groups of workers. a comparison by scanning electron microscopy of these packets provided a possibly consistent interpretation for the seemingly conflicting opinions whether tua were involved in packet induction. it was strongly suggested that the packets having tua in the wall were rigidly maintained by a bridging structure of th ...19902280726
the use of dna probes for confirming enterotoxin production by staphylococcus aureus and micrococci.dna-dna colony hybridization was employed to evaluate the results obtained by different immunological methods for detection of staphylococcal enterotoxin. staphylococcus aureus strains tested for staphylococcal enterotoxin production by immuno-assays and micrococci not previously tested for staphylococcal enterotoxin production were examined for presence of the genes encoding for staphylococcal enterotoxin a, b, c and e by using three corresponding dna probes. the staphylococcal enterotoxin a pr ...19902282285
antistaphylococcal activity of pentamidine.pentamidine isethionate was bacteriostatic against staphylococcus aureus, staphylococcus epidermidis, streptococcus pyogenes, streptococcus sanguis, micrococcus sp., and candida albicans. s. aureus was inhibited by concentrations of 16 to 64 micrograms/ml and killed by 64 to greater than or equal to 128 micrograms/ml. staphylococcal killing was consistently greater in the presence of cations and was unaffected by methicillin resistance.19902285292
bacterial endocarditis at a small community hospital.clinical features, microbiology, and predisposing factors are described in 56 patients with bacterial endocarditis (be) treated over a 12-year period at a small community hospital in hawaii. the average age of patients was 52.0 years. the mean duration of symptoms was 28.8 days (range 1 to 240 days). streptococci was the most frequently identified causative organism, present in 61% of the cases. gram-negative bacilli were isolated from six patients (11%). fourteen patients (25%) required cardiac ...19902301455
multilayer adsorption of lysozyme on a hydrophobic substrate.macromolecular adsorption is known to occur as a complex process, often in a series of steps. several models are discussed in the literature which describe the microscopic structure of the adsorbate. in the present study we investigated the adsorption of hen egg white lysozyme on alkylated silicon oxide surfaces. a combination of fluorescence excitation in the evanescent field and fluorescence recovery after photobleaching allowed us to measure the amount of adsorbed fluorescent lysozyme and the ...19902306502
microbial keratitis and corneal ulceration associated with therapeutic soft contact lenses.we reviewed the records of 22 patients whose corneal ulcers were associated with therapeutic soft contact lens wear. the patients required hospitalization on the cornea service at wills eye hospital between january 1, 1978 and september 1, 1988. a majority of the ulcers were associated with pseudophakic or aphakic bullous keratopathy (9 of 22 cases; 41%); neurotrophic/exposure keratitis was the second most common diagnosis (7 of 22; 32%). most patients used topical antibiotics (15 of 22; 68%) an ...19902306853
organomercurial-volatilizing bacteria in the mercury-polluted sediment of minamata bay, japan.a total of 4,604 bacterial strains isolated from the sediments of minamata bay and nearby low-level-mercury stations (control stations) were screened for the ability to volatilize mercury from inorganic and organic mercurial compounds. the strains that volatilize mercury from several kinds of organomercurials were found only in the sediments of minamata bay.19902310185
genetic and biochemical characterization of the cho-uv-1 mutant defective in postreplication recovery of dna.the cho-uv-1 mutant, a chinese hamster ovary cell with defective postreplication recovery of dna, is 2- to 4-fold more sensitive than its wild-type counterpart (cho-77256) to the lethal effects of ethylating agents and uv radiation; it is also hypersensitive (10- to 20-fold) to some dna-methylating and -cross-linking agents. we studied the cho-uv-1 mutant further to define its phenotype in terms of dna damage induction and repair, methyltransferase activity, and effects of caffeine on mutational ...19902317821
manganese(ii) induces cell division and increases in superoxide dismutase and catalase activities in an aging deinococcal culture.addition of mn(ii) at 2.5 microm or higher to stationary-phase cultures of deinococcus radiodurans ir was found to trigger at least three rounds of cell division. this mn(ii)-induced cell division (mn-cd) did not occur when the culture was in the exponential or death phase. the mn-cd effect produced daughter cells proportionally reduced in size, pigmentation, and radioresistance but proportionally increased in activity and amount of the oxygen toxicity defense enzymes superoxide dismutase and ca ...19902318808
microbial ecology of interdigital infections of toe web spaces.the microbial flora of normal and macerated interdigital toe web spaces was qualitatively and quantitatively identified in 77 patients. dermatophyte fungi were recovered from 11% of normal patients compared with a 31% recovery from patients with macerated interspaces. macerated interspaces were characterized by a greater recovery of organisms with pathogenic potential, with staphylococcus aureus recovered from 36% of patients, micrococcus sedentarius in 37%, brevibacterium epidermidis in 54%, co ...19902319017
substrate-mediated purification and characterization of a 3-hydroxybenzoic acid-6-hydroxylase from micrococcus.3-hydroxybenzoic acid-6-hydroxylase from micrococcus sp. was purified to homogeneity in a single step using the substrate-mediated interaction of the enzyme with blue-sepharose. the enzyme was bound to the affinity matrix in the presence of 3-hydroxybenzoic acid and was eluted in its absence. the molecular weight of the purified enzyme is 70,000 with no subunit structure. the flavoenzyme required the exogenous addition of fad for its complete activity and had a strict preference for nadh over na ...19902321959
cell surface of a tetrads-forming mutant of micrococcus luteus: chemical treatment of the cells and teichuronic acids on the surface.tetrads-forming mutant mt cells of micrococcus luteus, both treated with chemical reagents and non-treated, were observed with a scanning electron microscope (sem). the agglutinability of the cells with antiserum containing anti-teichuronic acid antibody was examined. the binding of protein a-gold particles to the cells, mediated with the antiserum, was also observed with sem. a tetrad surface, not surface of each of four "unit monococci" constituting a tetrad, consisted of two or three smooth a ...19902325580
role of gc-biased mutation pressure on synonymous codon choice in micrococcus luteus, a bacterium with a high genomic gc-content.the gc (g + c, or g or c)-contents of codon silent positions in all two-codon sets and three codons auy/a (iie), and in most of the family boxes of micrococcus luteus (genomic gc-content: 74%) are 95% to 100% in both the highly and weakly expressed genes. in some family boxes, there is a decrease in nnc codons and an increase in nng codons from the highly expressed to weakly expressed genes without apparent involvement of nnu and nna codons. from these observations, we conclude that the selectiv ...19902326195
glycosidases in brachionus plicatilis (rotifera).1. tests for glycosidases were performed in homogenates of brachionus plicatilis. 2. hydrolytic activity was detected with the following substrates: (a) with synthetic substrates (np = 4-nitrophenyl): np-alpha- and np-beta-d-glucopyranoside, np-alpha- and np-beta-d-galactopyranoside, np-n-acetyl-beta-d-glucosaminide, np-n-acetyl-beta-d-galactosaminide, np-alpha- and np-beta-d-mannopyranoside and np-alpha-l-fucopyranoside; (b) with disaccharides: sucrose, maltose, trehalose, isomaltose, cellobios ...19902328573
teichuronic acid reducing terminal n-acetylglucosamine residue linked by phosphodiester to peptidoglycan of micrococcus luteus.teichuronic acid-peptidoglycan complex isolated from micrococcus luteus cells by lysozyme digestion in osmotically stabilized medium was treated with mild acid to cleave the linkage joining teichuronic acid to peptidoglycan. this labile linkage was shown to be the phosphodiester which joins n-acetylglucosamine, the residue located at the reducing end of the teichuronic acid, through its anomeric hydroxyl group to a 6-phosphomuramic acid, a residue of the glycan strand of peptidoglycan. 31p nucle ...19902332401
artery, periarterial adipose tissue, and blood microbiology during vascular reconstructive surgery: perioperative and early postoperative observations.the presence and clinical significance of bacteria in the arterial wall, periarterial adipose tissue, and blood samples acquired during elective vascular operations were assessed in this study. specimens were obtained from a random series of 84 patients (56 men, 28 women) undergoing 75 primary and 9 secondary arterial reconstructions. operations performed most frequently included abdominal aortic aneurysmectomy (42), aortofemoral bypass reconstruction (15), and lower extremity bypass surgery for ...19902335832
chemical treatments of the cell packets induced from a micrococcus luteus mutant and teichuronic acids on the packet surface.cell packets (mt packets) induced from a tetrads-forming mutant (strain mt) of micrococcus luteus, both treated with chemical reagents and non-treated, were observed with a scanning electron microscope (sem). the agglutinability of mt packets with antiserum containing anti-teichuronic acid antibody was examined. the binding of protein a-gold particles to the mt packets, mediated with the antiserum, was also observed with sem. gold particles were observed uniformly on the whole packet surface and ...19902337910
wavelength dependence for uv-induced pyrimidine dimer formation in dna of human peripheral blood lymphocytes.an action spectrum for the induction of pyrimidine dimers in human peripheral lymphocytes was determined between 254 and 405 nm. the presence of pyrimidine dimers was determined as uv-induced lesions that were sensitive to the dimer-specific endonuclease from micrococcus luteus in conjunction with agarose gel electrophoresis. the rate of induction of pyrimidine dimers was maximal at 254 nm. these values can be compared with action spectra for uv-induced in vitro responses of lymphocytes.19902342505
replication, transcription and nuclease digestion of the unusual x-dna double helix of poly(amino2da-dt).the alternating copolymer poly(amino2da-dt) isomerizes into the unusual x-dna double helix at low-salt aqueous conditions (vorlicková et al., j. biomolec. struct. dyn. 6, 503-510 (1988)). this observation allowed us to start studies on how the x-dna is recognized, copied and hydrolyzed by various enzymes. in the present paper x-dna replication, transcription and digestion by various polymerases and nucleases, respectively, are examined and compared to appropriate controls. it is found that x-dna ...19902360999
modulation of the b-a transition of dna by potential antitumor antibiotics. influence of the base composition of dna.the b-a transition of dna in oriented films of dna-drug complexes is more or less restricted as a consequence of drug binding as revealed by infrared linear dichroism. a fraction of dna is irreversibly locked into the b form. this behavior is described by the number of dna base pairs "frozen" in the b form by one drug molecule. this quantity is dependent on the dna sequence the drug is attached to. in this paper, drug complexes of oriented films of nadna with a gc content of 42% from calf thymus ...19902361002
purification, amino acid sequence, and some properties of rabbit kidney lysozyme.the lysozyme (rabbit kidney lysozyme) from the homogenate of rabbit kidney (japanese white) was purified by repeated cation-exchange chromatography on bio-rex 70. the amino acid sequence was determined by automated gas-phase edman degradation of the peptides obtained from the digestion of reduced and s-carboxymethylated rabbit lysozyme with achromobacter protease i (lysyl endopeptidase). the sequence thus determined was kiyercelartlkklgldgykgvslanwmclakwessyntratnynpgdkstdygifq insrywcndgktpravn ...19902361954
quantitation of ultraviolet radiation-induced cyclobutyl pyrimidine dimers in dna by video and photographic densitometry.we have compared video and photographic methods for calculating the number of ultraviolet radiation (uv)-induced pyrimidine dimers in dna from the bacteriophage t7 exposed to uv (0 to 800 j/m2) from an fs40 sunlamp. dna was incubated with a pyrimidine dimer-specific micrococcus luteus uv endonuclease, subjected to alkaline agarose gel electrophoresis, neutralized, and stained with ethidium bromide, and the dna fluorescence was recorded either with a video camera or on photographic film. the slop ...19902363492
photochemistry of dna using 193 nm excimer laser radiation.photoproducts in double-stranded dna induced by 193 nm radiation have been investigated. double-stranded, supercoiled pbr322 dna in buffered aqueous solution was exposed to varying fluences of 193 nm radiation from an arf excimer laser. the quantum yields for formation of cyclobutylpyrimidine dimers, frank strand breaks and alkali labile sites were calculated from the conversion of supercoiled (form i) dna to relaxed (form ii) dna after treatment with micrococcus luteus dimer-specific endonuclea ...19902367550
differences in the phagocytosis of four bacteria by channel catfish neutrophils.the purpose of this study was to compare the phagocytosis of aeromonas hydrophila, edwardsiella ictaluri, edwardsiella tarda and micrococcus luteus by channel catfish neutrophils. various aspects of opsonization effect and bactericidal ability of channel catfish neutrophils were investigated. percent phagocytosis ranged from a low of 1% to a high of 91%. the highest percent phagocytosis and phagocytic indices routinely occurred with normal serum and were highest for m. luteus (91.78%, 55.25) and ...19902369968
effect of organic amendments on bacterial multiplication in lake water.in cayuga lake water amended with 30 micrograms of glucose or amino acids per ml, an added strain of pseudomonas fluorescens and indigenous bacteria grew extensively, pseudomonas sp. b4 and two rhizobia multiplied at a moderate extent, and introduced escherichia coli and klebsiella pneumoniae multiplied but to only a slight degree. the amendments did not enhance growth of micrococcus flavus and arthrobacter citreus, and an asporogenous strain of bacillus subtilis decreased in numbers. the pseudo ...19902372214
production of bacteriolytic activity in the oral cavity by nutritionally variant streptococci.microorganisms from the oral flora were examined for the production of bacteriolytic substances. among human viridans group streptococci, only one group of strains with thiol-dependent properties was shown to secrete enzymes with bacteriolytic activity on heat-killed cells of micrococcus luteus on double-layer nutrient agar plates. by morphology, culture requirements, and biochemical properties, they were found to conform to descriptions of nutritionally variant streptococci (nvs). bacteriolytic ...19902380384
self-leveling mortar as a possible cause of symptoms associated with "sick building syndrome".in newly constructed houses and buildings in which self-leveling mortar containing casein has been used, residents and office employees have noted a bad odor and have complained of headache, eye and throat irritation, and tiredness. these problems were suspected to result from the degradation products emitted from the mortar. samples obtained from dry mortar powder and from mortar in buildings where casein was used and from control buildings were found to contain microorganisms (mean of 10(2) cu ...19902386418
polymer length of teichuronic acid released from cell walls of micrococcus luteus.teichuronic acid released from its phosphodiester linkage to peptidoglycan in the cell walls of micrococcus luteus by mild acid treatment is resolved into a ladderlike series of bands by electrophoresis on polyacrylamide gels in the presence of borate. each band of the ladder differs from its nearest neighbor by one disaccharide repeat unit, ----4)-2-acetamido-2-deoxy-beta-d-mannopyranuronosyl-(1----6)- alpha-d-glucopyranosyl-(1-. acid-fragmented teichuronic acid, after conversion to the phenyla ...19902394683
molecular recognition in the minor groove of the dna helix. studies on the synthesis of oligonucleotides and polynucleotides containing 3-deaza-2'-deoxyadenosine. interaction of the oligonucleotides with the restriction endonuclease ecorv.an improved procedure for the preparation of 3-deaza-2'-deoxyadenosine (d3ca) is described which is suitable for the synthesis of gram quantities of this analogue. using phosphoramidite chemistry d3ca has been incorporated into the eco rv restiction endonuclease recognition sequence (underlined) present in the self-complementary dodecamer d(gacgatatcgtc). the modified oligonucleotides have been thoroughly characterised by nucleoside composition analysis, circular dichroism and thermal melting st ...19902395641
the bacteriology of fresh and spoiling lake victorian nile perch (lates niloticus).a total of 177 bacterial cultures isolated from lake victorian nile perch (lates niloticus) were investigated. the flora on newly caught nile perch consisted of organisms belonging to the genera moraxella, alcaligenes, acinetobacter, pseudomonas, aeromonas, micrococcus and other gram-positive organisms. 39% were identified as gram-positive species and 61% were negative in the gram-reaction. three cultures out of 53 investigated caused weak rotten off-odours in sterile fish broth and one culture, ...19902397157
microbial challenge of a blood cell separator outside-seal bowl system.the ability to store platelets beyond 24 hours requires a functionally closed system. this study tested the ability of a cell separator bowl seal system to resist penetration of microbial contamination under normal running conditions and under extreme environmental stress. three test organisms, micrococcus luteus, serratia marcescens, and staphylococcus epidermidis, were applied directly to the bowl at the edge of the seal or aerosolized and passed through the centrifuge chamber while the cell s ...19902407000
template properties of ultraviolet-irradiated poly(dc) replicated by e. coli dna polymerase i: indication for a role of apyrimidinic-sites in uv-induced mutagenesis.ultraviolet irradiation alters the template properties of poly(dc) when replicated by escherichia coli dna polymerase i. these effects are due to base modifications. some of them are identified as apurinic/apyrimidinic sites (ap-sites) by their sensitivity to ap-endonuclease b purified from micrococcus luteus, and their template properties. the rate of formation of ap-sites in poly(dc) is estimated at 3 x 10(-7) site per nucleotide per j.m-2. exposure of supercoiled or relaxed pbr322 dna to uv l ...19852412595
[composition and properties of the outer cell wall layer in bacillus brevis--the producer of gramicidin s].two membrane antigens were found by cross immunoelectrophoresis in the cell walls of bacillus brevis var. g.-b., r form, which started to synthesize gramicidin s (20 mg per 1 ml of cultural broth). the cell wall contained no membrane components in cells at the beginning of the logarithmic growth phase. the protein with a molecular mass of 100 kda is a component of the cell wall outer layer. the protein is not digested by trypsin or pronase when it comprises the cell walls of cells synthesizing g ...19852413338
[reactivity to bacterial peptidoglycans in a phagocytosis system. the range of immunological specificity of escherichia coli peptidoglycan].the antigenic specificity of e. coli m17 peptidoglycans (pg) was studied in the test system based on the stimulation of neutrophils with pg opsonized with the whole and exhausted igg fraction of human serum. within the generic taxon, the pg under study were similar in their antigenic properties. the opsonic activity of igg exhausted with the preparations of different e. coli strains decreased by 93.2-95.8%. an essential difference of e. coli pg from similar components of other representatives of ...19852413663
[effect of phosphates on the membrane activity of gramicidin s].the membrane activity of gramicidin s against intact cells of staphylococci and micrococci determined by the loss of intracellular low-molecular compounds with the adsorption maxima at 260 nm by the bacteria markedly increased in the presence of phosphates. acetate and hydrochlorides had no effect on the membranotropic action of the antibiotic. analogous results were obtained for gramicidin s derivatives by free ornithine amino groups possessing basic and antibiotic activity. the increased membr ...19852413800
[action of different types of gramicidin s derivatives on bacterial cells and protoplasts].the work was concerned with studying the effect of gramicidin s derivatives with modified free amino groups of ornithine residues on bacterial cells and protoplasts. the substitution of the amino groups with neutral or carboxyl-containing groups eliminated or sharply decreased the antibacterial activity of gramicidin s, its binding to the cells, and the ability to change the permeability of the cytoplasmic membranes of the intact cells. however, the neutral derivatives and the derivative with ac ...19852414637
an unusual central nervous system infection in a young immunocompromised host.a 13-year-old girl with a ten-year history of lymphoblastic leukemia and several central nervous system (cns) relapses developed a bone marrow relapse and accelerated cns leukemia. following treatment with cns radiation and intravenous chemotherapy, she developed fever, pancytopenia, headache, and vomiting. her neurological function deteriorated and she died on the 20th hospital day. multiple csf examinations failed to disclose either leukemic cells or organisms. blood cultures obtained from a b ...19862423054
[thermal inactivation and stabilization of lysozyme substrate-- micrococcus lysodeicticus cells].heat inactivation of the acetonic powder of micrococcus lysodeicticus cells suspended in phosphate buffer ph 6.2 was quantitatively characterized in the temperature range from 34 to 52 degrees. the total value of the rate constant for heat inactivation of the cells equals 2.88 x 10(8) exp(-18360/rt) sec-1. the activation parameters of the process at 34 degrees are the following: delta h* = 17.7 kcal/mole; delta s* = 21.8 e. u.; delta f* = 24.4 kcal/mole. the effect of ethylene glycol, mannitol, ...19862424002
down-regulation of macrophage lysozyme by lipopolysaccharide and interferon.lipopolysaccharide (lps) treatment of resident mouse peritoneal macrophages (m phi) was found to suppress intracellular as well as secreted lysozyme (lzm). interferon (ifn) had a similar effect. lzm was identified by the capacity of cell lysates or medium to lyse micrococcus lysodeikticus, and by the presence of a 14.5 kd protein band which co-migrated with human lzm in sds-page and which reacted positively in western blots with antiserum to human lzm. the size of the 14.5 kd band decreased sequ ...19862424976
the repair of pyrimidine dimers via a dna-glycosylase mechanism.the "uv endonuclease" isolated either from m. luteus or bacteriophage t4 infected e. coli (the denv gene product) consists of two enzymatic activities on a single polypeptide chain: a pyrimidine dimer-dna glycosylase and an ap endonuclease. the repair of pyrimidine dimers by this enzyme is initiated by the cleavage of the n-glycosylic bond of the 5' pyrimidine of the dimer that leaves the cyclobutane dimer still attached to the dna through the n-glycosylic bond of the 3' pyrimidine of the dimer. ...19862427065
analysis of cell division gene ftsz (sulb) from gram-negative and gram-positive bacteria.the ftsz (sulb) gene of escherichia coli codes for a 40,000-dalton protein that carries out a key step in the cell division pathway. the presence of an ftsz gene protein in other bacterial species was examined by a combination of southern blot and western blot analyses. southern blot analysis of genomic restriction digests revealed that many bacteria, including species from six members of the family enterobacteriaceae and from pseudomonas aeruginosa and agrobacterium tumefaciens, contained seque ...19872432055
[the capacity of anaerobically grown bacteria to exchange the 2h+ of the cell for the k+ of the medium and to maintain a high k+ distribution between cell and medium].the n,n'-dicyclohexylcarbodiimide sensitive exchange of 2h+ of a cell for k+ of medium stable to ph, k+ activity and temperature changes has been discovered in anaerobically grown gram-negative escherichia coli, salmonella typhimurium. s. enteritidis, proteus mirabilis, p. vulgaris, anaerobic gram-positive bacteria streptococcus faecalis, lactobacillus salivarius, l. lactis in the presence of exogenic energy source. this exchange in gram-negative bacteria is operating only at increase of medium ...19862434147
[comparative study of the surface-active properties of gramicidin s and its derivatives by polarography].gramicidin s and its derivatives by the free amino groups of the ornithine moieties were studied with respect to their effect on interface tension of mercury and the polarographic background for comparative estimation of the surface active properties of these compounds. the same way as the compounds preserving their main properties, the neutral and acid derivatives lowered the polarographic maximum. no correlation between the level of the antimicrobial and membrane activity and the surface activ ...19862436573
isolation and characterization of an activator for azotobacter vinelandii nicotinamide mononucleotide glycohydrolase.azotobacter vinelandii nmn glycohydrolase [ec 3.2.2.14] has been shown to require absolutely gtp or a high-molecular-weight and heat-stable component for its function. the intracellular activator could be purified from its sonicate by heat treatment, acetone precipitation, phenol extraction, and acid precipitation in a good yield. the purified activator showed high affinity and effectiveness for nmn glycohydrolase (ka = 0.012 optical density unit at 257 nm/ml; vmax standardized by the activity a ...19872437104
antibodies to various bacterial cell wall peptidoglycans in human and rabbit sera.sera from patients with verified systemic staphylococcal infection contained antibodies reactive with peptidoglycan (pg) from staphylococcus aureus, staphylococcus epidermidis, streptococcus pyogenes, micrococcus lysodeikticus, bacillus subtilis, and escherichia coli. the presence of anti-pg cross-reactive antibodies was verified in patient sera by inhibition studies with the various bacterial pgs. antibodies to nonstaphylococcal pgs were also elevated in sera from rabbits immunized with s. aure ...19872437150
selective inhibition by methoxyamine of the apurinic/apyrimidinic endonuclease activity associated with pyrimidine dimer-dna glycosylases from micrococcus luteus and bacteriophage t4.the uv endonucleases [endodeoxyribonuclease (pyrimidine dimer), ec 3.1.25.1] from micrococcus luteus and bacteriophage t4 possess two catalytic activities specific for the site of cyclobutane pyrimidine dimers in uv-irradiated dna: a dna glycosylase that cleaves the 5'-glycosyl bond of the dimerized pyrimidines and an apurinic/apyrimidinic (ap) endonuclease that thereupon incises the phosphodiester bond 3' to the resulting apyrimidinic site. we have explored the potential use of methoxyamine, a ...19872443160
induction and repair of closely opposed pyrimidine dimers in saccharomyces cerevisiae.pyrimidine dimer-dna glycosylase activity prepared from micrococcus luteus has been used to develop an enzyme-sensitive site assay for the detection and quantification of closely opposed pyrimidine dimers in the nuclear dna of uv-irradiated yeast. with this assay, closely opposed dimers were found to be induced as a linear function of dose from 0 to 200 j/m2 (254 nm). closely opposed dimer frequencies decreased during the incubation of uv-irradiated, excision repair-proficient cells under liquid ...19872444878
directional mutation pressure and transfer rna in choice of the third nucleotide of synonymous two-codon sets.bacterial species have diverged into a series of families, some with high g + c content in their dna, and other with high a + t content, resulting, respectively, from g.c- and a.t-directional mutation pressures. such mutation pressure (g.c/a.t pressure) may be an important determinant for codon usage. it has also been suggested that trna acts as a selective constraint for determining codon usage. we have studied the relation between g.c/a.t pressure and trna constraints in determining choice of ...19882448791
dna probes with different specificities from a cloned 23s rrna gene of micrococcus luteus.a 7500 bp psti restriction fragment of chromosomal dna from micrococcus luteus containing a 23s rrna gene was cloned in vector phe3 in e. coli rr 28 (the recombinant plasmid was designated par1). a recombinant phage (par5) hybridizing to all eubacteria tested was constructed by shotgun subcloning of the psti fragment in phage m13mp8. further subcloning of the fragments of the 23s rrna gene in the vectors ptz18r and ptz19r using selected restriction sites of the gene enabled us to select cloned f ...19882462009
[effect of salts on the lytic activity of gramicidin s and its derivatives].potassium and sodium chlorides, sulfates, acetates and phosphates activated the lytic action of gramicidin s and its derivatives on protoplasts of m. lysodeikticus. the derivatives used were positively charged and neutral by the free amino groups in the ornithine moieties. the salts had no effect on lysis of the bacillar protoplasts by gramicidin s and its positively charged derivatives. the lytic effect of the neutral derivative on the bacillar protoplasts markedly increased in the presence of ...19882464340
degradation of bacterial cell walls by immobilized lysozyme.lysozyme was immobilized by two different methods in two different ways in order to obtain a preparation with an activity as high as possible toward a macromolecular substrate. the enzyme was bound as a schiff base to a silicate carrier by using oxidized dextrans of different lengths as spacer and also was bound to controlled pore aminoglass via pyridino-4-aldehyde and brcn. the latter preparations had activities up to 2.5% of the free lysozyme.19882469390
bacteriophage-t4 and micrococcus luteus uv endonucleases are not endonucleases but beta-elimination and sometimes beta delta-elimination catalysts.bacteriophage-t4 uv endonuclease nicks the c(3')-o-p bond 3' to ap (apurinic or apyrimidinic) sites by a beta-elimination reaction. the breakage of this bond is sometimes followed by the nicking of the c(5')-o-p bond 5' to the ap site, leaving a 3'-phosphate end; delta-elimination is proposed as a mechanism to explain this second reaction. the ap site formed when this enzyme acts on a pyrimidine dimer in a polynucleotide chain undergoes the same nicking reactions. micrococcus luteus uv endonucle ...19892471512
mechanism of polynucleotide phosphorylase.the de novo polymerization of rna initiated by polynucleotide phosphorylase from nucleoside diphosphates was examined. end group analysis performed under conditions designed to specifically end label the polymer revealed no evidence for a 5'-pyrophosphate-terminated polymer. however, we observed preferential incorporation of the adp alpha s(rp) diastereomer into the 5' end (marlier & benkovic, 1982) in chain initiation, suggesting that the enzyme incorporates a nucleoside diphosphate specificall ...19892476178
a possible role of chromatin and tightly-bound chromatin proteins on enzyme-catalyzed methylation of dna.upon extensive digestion with dnaasei of placenta chromatin matrix, previously "stripped" from its loosely-bound components by high-salt extraction, a fraction is obtained that contains almost no endogenous dna methylase activity but whose dna, if still included in this whole fraction--not if it has been purified to a protein-free condition--is a good substrate for externally added enzyme. this chromatin matrix can even cause a significant stimulation of methylation of single-stranded micrococcu ...19892476222
modulation of the dna scanning activity of the micrococcus luteus uv endonuclease.micrococcus luteus uv endonuclease incises dna at the sites of ultraviolet (uv) light-induced pyrimidine dimers. the mechanism of incision has been previously shown to be a glycosylic bond cleavage at the 5'-pyrimidine of the dimer followed by an apyrimidine endonuclease activity which cleaves the phosphodiester backbone between the pyrimidines. the process by which m. luteus uv endonuclease locates pyrimidine dimers within a population of uv-irradiated plasmids was shown to occur, in vitro, by ...19892477371
[study of the adaptation resistance in bacteria to membrane active polypeptide antibiotics].variants of micrococcus lysodeikticus resistant to 100 micrograms/ml of gramicidin s with preserved resistance in subcultures on media without the antibiotic were isolated as a result of prolonged adaptation on a solid medium with increasing concentrations of gramicidin. the sensitive and resistant cells did not differ by their ability to bind gramicidin. under the antibiotic effect permeability of the cytoplasmic membranes of the intact cells in the sensitive bacteria appeared to be impaired to ...19892478091
micrococcus luteus, a bacterium with a high genomic g + c content, contains escherichia coli-type promoters.the g + c content of micrococcus luteus dna is 74%, which is much higher than that of escherichia coli (about 50%). in order to understand the influence of gc-directed mutation pressure (gc pressure) on the promoter structure, the initiation sites for both in vitro and in vivo transcription of m. luteus streptomycin (str) and spectinomycin (spc) operons were identified by the reverse transcriptase mapping method. the promoter sequences of m. luteus are similar to those of e. coli, but have signi ...19892479819
flow microcalorimetric assay of antibiotics--iii. zinc bacitracin and its combinations with polymyxin b sulphate and neomycin sulphate on interaction with micrococcus luteus.a flow microcalorimetric assay for zinc bacitracin has been developed which has better reproducibility (relative standard deviation less than 2%) and sensitivity (0.02 micrograms ml-1) than conventional microbiological assays, and requires an assay time of between 7.5-9 h. the assay is not suitable for zinc bacitracin determinations in the presence of equimolar concentrations of polymyxin b sulphate or neomycin sulphate, or of these antibiotics in the proportions in which they occur in the comme ...19892490095
a structural requirement in the subsite f of lysozyme. the role of arginine 115 in human lysozyme revealed by site-directed mutagenesis.arginine 115 in the subsite f of human lysozyme (peptidoglycan n-acetylmuramoylhydrolase, ec 3.2.1.17) was replaced with lysine, histidine, glutamine or glutamine acid by site-directed mutagenesis. the conversions which conserve positive charge, arg115 to lys or his (at acidic ph), have little affected on either the kinetic parameters for micrococcus lysodeikticus cells or the activity against glycol chitin, nor on the cleavage patterns of hexa(n-acetylglucosamine) [(glcnac)6] and penta(n-acetyl ...19892493374
[investigations on the standardization of the lysoplate technique for assay of lysozyme].in this paper studies for the standardization of the estimation of lysozyme in low concentrations concerning the lysoplate technique are reported. the influence of the conditions of diffusion, the concentration and the age of the bacteria as well as the judgement of different pictures of lysis and contrast resp. have been measured and compared. sufficient results have been obtained with "purified agar" (difco) in a concentration of 0.9% and with living, 48 h old strains from micrococcus luteus. ...19892497626
excision-repair capacity of uv-irradiated strains of escherichia coli and streptococcus pneumoniae, estimated by plasmid recovery.although the biological role of many bacterial repair genes is known, there is still an interest in evaluating the capacity of repair pyrimidine dimers in some strains. for this purpose, we have developed a rapid assay. cells bearing a plasmid are uv irradiated and incubated to allow recovery. the plasmid dna is extracted, purified and treated with uv endonuclease from micrococcus luteus that specifically produces single strand breaks at the site of pyrimidine dimers. the amount of open circular ...19892498485
[efficient hygiene precautions in the household today].the results of epidemiological investigations justifies the assumption that increasing health defects, especially enteritis infectiosa, are caused inter alia by inadequate hygienic conditions in households. the number of such diseases ranges between 100,000 and more than 1,000,000 cases per year in the frg. responsible for this development is a lack of information about the behaviour of microorganisms in the environment and its pathways of distribution. in addition risks are growing with the rec ...19892500806
influence of cationic peptides on the activity of the autolytic endo-beta-n-acetylglucosaminidase of staphylococcus simulans 22.the peptidoglycan hydrolyzing endo-beta-n-acetylglucosaminidase of staphylococcus simulans 22 is not able to attack intact cell walls of s. simulans 22, but hydrolyzes cell walls of micrococcus luteus and soluble peptidoglycan chains of s. simulans 22. hydrolysis of cell walls of m. luteus is activated in presence of organic cations such as poly-l-lysine (n = 17) and the peptide antibiotics pep 5 and nisin, whereas hydrolysis of soluble peptidoglycan chains is not influenced. high concentrations ...19892501145
[antibacterial spectrum of lisosubtilin g10x].data on the antibacterial spectrum of lysosubtilin g10x, a preparation of lytic enzymes from bacillus subtilis sk-52 are presented. lysosubtilin was active against grampositive and gramnegative pathogenic bacteria. when it was used as a substrate of live lyophilized microbial cells the highest lysis levels were observed in b. brevis, b. cereus, b. pumilis, b. subtilis and s. faecalis. preincubation of the substrate in acid media mainly increased the levels of the lysis by enzyme preparation. som ...19892511813
evaluation of four qualitative methods for detection of beta-lactamase production in staphylococcus and micrococcus species.four qualitative methods for the detection of beta-lactamase production in staphylococcus and micrococcus species were evaluated and compared with a quantitative macroiodometric reference method. the disc diffusion test with penicillin g and the cloverleaf method could not separate beta-lactamase-positive from beta-lactamase-negative strains. two applications of the chromogenic cephalosporin test, using uninduced strains and strains grown on blood agar plates, gave a large number of false negati ...19892513196
template-primer activity of 5-(hydroxymethyl)uracil-containing dna for prokaryotic and eukaryotic dna and rna polymerases.we have utilized bacillus subtilis phage spo-1 dna as a model of irradiated dna. in this phage, all thymine (thy) residues are replaced by 5-(hydroxymethyl)uracil (5hmura), which is a known irradiation-induced derivative of dna thy. spo-1 phage is naturally devoid of other such irradiation-induced dna lesions. dnase i activated spo-1 phage dna served as well as, or even better than, the control dnas (bacillus subtilis dna and calf thymus dna) as a template-primer for escherichia coli, micrococcu ...19892513877
purification of a 51 kda endo-beta-n-acetylglucosaminidase from staphylococcus aureus.a bacteriolytic enzyme obtained from the culture fluid of staphylococcus aureus fda 209p was purified to homogeneity utilizing dye-ligand affinity column chromatography, hydrophobic interaction high pressure liquid chromatography (hplc) and hydroxyapatite hplc. subsequent characterizations indicated that the purified enzyme acted as endo-beta-n-acetylglucosaminidase. the molecular weight determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page) was 51,000 and the isoele ...19892515095
sequence alignment and evolutionary comparison of the l10 equivalent and l12 equivalent ribosomal proteins from archaebacteria, eubacteria, and eucaryotes.the genes corresponding to the l10 and l12 equivalent ribosomal proteins (l10e and l12e) of escherichia coli have been cloned and sequenced from two widely divergent species of archaebacteria, halobacterium cutirubrum and sulfolobus solfataricus. the deduced amino acid sequences of the l10e and l12e proteins have been compared to each other and to available eubacterial and eucaryotic sequences. we have identified the human p0 protein as the eucaryotic l10e. the l10e proteins from the three kingd ...19892515294
[the effect of specific inducers on the biosynthesis of lytic enzymes by a strain of streptomyces recifensis var. lyticus].the effect of specific inductors at different stages of streptomyces recifensis var. lyticus 2435 cultivation was studied. it is shown that introduction of killed bacterial cells into inoculum not only influences the level of accumulation of lytic enzymes by the strain s. recifensis var. lyticus 2435 but also determines the qualitative composition of the synthesized complex. the yield of bacteriological glycosidases grows with introduction of the micrococcus lysodeikticus cells into the inoculum ...19892516231
rolling-circle replication of uv-irradiated duplex dna in the phi x174 replicative-form----single-strand replication system in vitro.cloning of the phi x174 viral origin of replication into phage m13mp8 produced an m13-phi x174 chimera, the dna of which directed efficient replicative-form----single-strand rolling-circle replication in vitro. this replication assay was performed with purified phi x174-encoded gene a protein, escherichia coli rep helicase, single-stranded dna-binding protein, and dna polymerase iii holoenzyme. the nicking of replicative-form i (rfi) dna by gene a protein was essentially unaffected by the presen ...19892524471
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