Publications

lcrf is the temperature-regulated activator of the yada gene of yersinia enterocolitica and yersinia pseudotuberculosis.	the virulence plasmid of human pathogenic yersinia species, pyv, encodes secreted proteins, yop proteins, and an outer membrane protein, yada. yada has been associated with binding to a variety of substrates and with interference with host defense. yada is regulated by temperature and is expressed only at 37 degrees c. unlike the yop regulon, the yada gene is not under ca2+ regulation. here, we show that lcrf (virf), the temperature-regulated activator of the yop regulon, also acts as an activat ...	1992	1548243
yersinia arthritis versus acute rheumatic fever in a boy.		1992	1549267
a new gene of the f1 operon of y. pestis involved in the capsule biogenesis.	the dna sequence determination of the f1 operon between the genes encoding the f1 subunit (caf1) and chaperone-like protein (caf1m) revealed a large open reading frame that codes for a polypeptide similar to some e. coli proteins involved in the biogenesis of fimbria. the deletion and in trans complementation analyses showed that this gene is not necessary for extracellular transport of the f1 subunit but plays a role in the capsule assembly.	1992	1551441
determination of genome size, macrorestriction pattern polymorphism, and nonpigmentation-specific deletion in yersinia pestis by pulsed-field gel electrophoresis.	of 16 restriction endonucleases known to hydrolyze rare 6- or 8-base recognition sequences that were tested, only spei, noti, asci, and sfii generated fragments of chromosomal dna from yersinia pestis, the causative agent of bubonic plague, of sufficient length to permit physical analysis by pulsed-field gel electrophoresis (pfge). of the individual bands detected after single-dimensional pfge of these digests, the largest sum was obtained with spei (3,575.6 +/- 114.6 kb). of these 41 bands, 3 w ...	1992	1551830
clinical and microbiologic characteristics of cutaneous infection with yersinia enterocolitica.	the clinical and microbiologic features of primary cutaneous infections by yersinia enterocolitica are described in three children. vesiculobullous lesions developed in two patients, and an intense granulation response followed incision and drainage. in the third child, cellulitis and abscess formation developed at the site of minor skin trauma. y. enterocolitica isolates from the patients were extensively serologically, biochemically, and molecularly analyzed and compared with virulent y. enter ...	1992	1552204
molecular cloning, iron-regulation and mutagenesis of the irp2 gene encoding hmwp2, a protein specific for the highly pathogenic yersinia.	under iron-starvation, the highly pathogenic yersinia synthesize several iron-regulated proteins including two high-molecular-weight polypeptides (hmwp1 and hmwp2). from the chromosome of yersinia enterocolitica serovar o:8 (strain ye 8081), the genes coding for the hmwp2 (irp2) and its promoter were cloned into plasmid puc18 (pir2) and used as a probe. we show here that the irp2 gene is present only in the highly pathogenic strains and that its promoter is iron-regulated in escherichia coli. af ...	1992	1552851
role of the transcriptional activator, virf, and temperature in the expression of the pyv plasmid genes of yersinia enterocolitica.	upon incubation at 37 degrees c in the absence of ca2+ ions, pathogenic yersiniae release high amounts of pyv plasmid-encoded proteins called yops, involved in pathogenesis. yersinia enterocolitica also express two outer membrane proteins, an adhesin called yada and a lipoprotein called ylpa. the production of the yops is co-ordinately regulated by a 20 kb region of the plasmid referred to as the 'ca2+ dependence region' and containing at least four loci called vira, virb, virc, and virf. the 8. ...	1992	1552853
cloning and characterization of the eae gene of enterohaemorrhagic escherichia coli o157:h7.	the eae (escherichia coli attaching and effacing) gene from enteropathogenic escherichia coli (epec) was previously shown to be essential for production of the 'attaching and effacing' histopathology characteristic of epec infections (jerse et al., 1990). we have now cloned the eae gene from enterohaemorrhagic e. coli (ehec) which, in addition to producing shiga-like cytotoxins, also produces the attaching and effacing effect. the sequence homology between the epec and ehec sequences was 86% and ...	1992	1552854
[emergent infections].		1992	1553065
pharyngitis and infections with yersinia enterocolitica.		1992	1553119
new clues to how bacteria get into cells.		1992	1553529
septicemia due to yersinia enterocolitica in a hemodialyzed, iron-depleted patient receiving omeprazole and oral iron supplementation.	septicemia occurred in a long-term hemodialysis patient on oral iron supplementation who had been treated for esophageal ulcer by omeprazole, an ulcer-healing drug. yersinia enterocolitica serotype 0:3 was recovered from blood cultures. a raised intraintestinal ph and an increased intraluminal iron load may have been contributing factors for the enhanced proliferation and generalized infection of y enterocolitica.	1992	1553973
[molecular and cellular bases of the virulence of shigella flexneri].	shigella flexneri, a gram negative bacillus, causes bacillary dysentery, an ulcerative disease of the human colon, by invading intestinal epithelial cells. entry into epithelial cells occurs via an induced phagocytic process which involves the actino-myosin complex. the host-cell receptor and the transmembrane signal which initiate reorganization of the cytoskeleton are under study. binding to integrins has recently been demonstrated in related models such as the entry of yersinia pseudotubercul ...	1992	1554236
molecular mimicry in the pathogenesis of spondyloarthropathies. a critical appraisal of cross-reactivity between microbial antigens and hla-b27.	we describe an amino acid homology between a virulence plasmid encoded outer membrane protein of yersinia, yada (previously called yop1) and hla-b27. this tetrapeptide is also included in the hexapeptide, earlier found to be identical between klebsiella nitrogenase and hla-b27. the synthetic peptide based on the hla-b27 homologous portion of the yada does not stimulate lymphocytes obtained from hla-b27+ patients with yersinia-triggered reactive arthritis or from controls. one-third of the yersin ...	1992	1555037
mechanistic studies of the biosynthesis of 3,6-dideoxyhexoses in yersinia pseudotuberculosis. purification and stereochemical analysis of cdp-d-glucose oxidoreductase.	an nad(+)-dependent cdp-d-glucose oxidoreductase which catalyzes the first step of the biosynthesis of cdp-ascarylose (cdp-3,6-dideoxy-l-arabino-hexose), converting cdp-d-glucose to cdp-4-keto-6-deoxy-d-glucose, was isolated from yersinia pseudotuberculosis. a protocol consisting of deae-cellulose, matrex blue-a, hydroxylapatite, deae-sephadex, sephadex g-100, and nad(+)-agarose column chromatography was used to purify this enzyme 6000-fold to homogeneity. this enzyme consists of two identical s ...	1992	1556102
visual identification of bacterially contaminated red cells.	there have been increasing numbers of reports of transfusion-acquired yersinia enterocolitica bacteremia (including several fatal cases). fifteen units of whole blood were inoculated with various concentrations of y. enterocolitica serotype 0:3 and processed into as-3 preserved red cells (rbcs). consistent growth of the organism was found at inoculum concentrations greater than or equal to 10 colony-forming units per ml. in all 13 units of rbcs that supported the growth of y. enterocolitica, a d ...	1992	1557802
inflammatory bowel diseases.		1992	1559499
parasitology. skirmishes on the border.		1992	1560820
do bacterial antigens cause reactive arthritis?	there are at least three common features among the microbes causing reactive arthritis: (1) they primarily cause infections on mucosal areas, (2) they are intracellularly living microorganisms, and (3) they have lipopolysaccharide as an essential structure of their outer membrane. it is obvious that during or after the acute infection microbial antigens are not properly eliminated, and they persist for long times in hla-b27-positive persons developing rea. bacterial lipopolysaccharide has also b ...	1992	1561408
antigenic responses in reactive arthritis.	in this article, the mechanisms by which infection at a distant site could lead to rea and whether they could explain the association of rea with hla-b27 have been discussed. we propose that rea synovitis is primarily due to specific synovial t-cell proliferation to fragments of the triggering bacterial found in the joint. nonspecific t cells amplify synovitis with antibodies playing only a secondary role. first, we have shown that the triggering bacterial antigen is present in a nonviable form ...	1992	1561409
yersinia enterocolitica: a new or unrecognized nosocomial pathogen?		1992	1564309
yersinia enterocolitica infections in hospitalized patients: the problem of hospital-acquired infections.	to study the epidemiology of yersinia enterocolitica infections in hospitalized patients and to determine the frequency of hospital-acquired infection and the modes of transmission within the hospital.	1992	1564310
neonatal yersinia enterocolitica enteritis.		1992	1565560
bacterial resistance to complement killing mediated by the ail protein of yersinia enterocolitica.	ail is a 17-kda outer membrane yersinia protein that mediates bacterial attachment to, and invasion of, cultured epithelial cells. we report here an alternative role for ail in the pathogenesis of yersinia infection. we found that escherichia coli hb101 harboring the 4-kilobase recombinant ail clone pvm102 were highly resistant to killing in up to 50% normal human serum. a 674-base-pair fragment of dna from pvm102, which encodes the ail gene, was inserted into puc18 and shown to promote full res ...	1992	1565652
comparison of hektoen and salmonella-shigella agar on 6033 stools of human origin submitted for routine isolation of salmonella sp. and shigella sp.	from 1989 through 1991, 6033 stool specimens from patients at orleans hospital (france), were cultured for enteric pathogens. hektoen agar (he) was compared with salmonella-shigella agar (ss) after direct streaking and after overnight enrichment in mueller-kauffmann broth. yersinia strains were not computed. seven strains of salmonella serotype typhi were isolated, on both media, and excluded from computations for discussed reasons. out of a total of 9 shigella strains, he isolated 9 and ss isol ...	1992	1570178
reactive arthritis of the cervical spine due to yersinia enterocolitica in a patient with preexisting ankylosing spondylitis.		1992	1570498
campylobacter enteritis in portugal: epidemiological features and biological markers.	from 1984 to 1989, stool samples from 2811 gastroenteritis cases were examined for the presence of campylobacter jejuni and c. coli, salmonella, shigella and yersinia species. isolation rates were: campylobacter jejuni and c. coli, 5.3%, salmonella spp., 14.8%, shigella spp., 4.6% and yersinia enterocolitica, 1.1%. age group distribution analysis shows a higher campylobacter isolation rate in children under one year of age. seasonal distribution revealed a peak incidence in winter as in other me ...	1992	1572427
monitoring of yersinia enterocolitica in murine and bovine feces on the basis of the chromosomally integrated luxab marker gene.	we previously integrated the luxab gene into the yersinia enterocolitica chromosome. in this article, we assessed, by luminometry, the survival of the engineered strain kng1024 in the digestive tracts of mice and cows. in situ detection and a count of the released strain were performed on feces from orally inoculated balb/c mice for 24 days. this method is a rapid and reliable system for long-term monitoring of genetically engineered bacteria. in cow feces, the count of y. enterocolitica ranged ...	1992	1575474
cloning and nucleotide sequence of the eae gene homologue from enterohemorrhagic escherichia coli serotype o157:h7.	the eae gene has recently been shown to be necessary for the attaching and effacing (ae) activity of enteropathogenic escherichia coli (epec) on intestinal epithelial cells. in this paper we report the cloning and nucleotide sequence of a similar gene from a strain of enterohemorrhagic e. coli (ehec) serotype o157:h7. an ehec eae sequence was identified which was 97% homologous to the epec eae gene for the first 2200 bp and 59% homologous over the last 800 bp. both eae sequences show 50% homolog ...	1992	1577255
a novel protein, lcrq, involved in the low-calcium response of yersinia pseudotuberculosis shows extensive homology to yoph.	the plasmid-encoded yop genes of pathogenic yersiniae are regulated by the environmental stimuli calcium and temperature. a novel protein, lcrq, which exhibits a key function in the negative calcium-controlled pathway, was identified. dna sequence analysis revealed that lcrq has a molecular mass of 12,412 daltons and its isoelectric point is 6.51. overexpression of lcrq in trans in wild-type yersinia pseudotuberculosis ypiii(pib102) changed the phenotype from calcium dependence to calcium indepe ...	1992	1577700
[yersiniosis, a multisystemic disease].		1992	1581448
influence of antibiotics on iga and igg response and persistence of yersinia enterocolitica in patients with yersinia-associated spondylarthropathy.	the iga and igg antibody response to plasmid-encoded outer membrane proteins was studied in 59 patients with yersinia-associated spondylarthropathy during 15 months of follow-up. initially, all patients had specific iga and igg antibodies to the 36 and 46 kda and 30% also to the 26 and 58 kda released proteins, which correlated with the finding of virulent yersinia bacilli in intestinal biopsies. iga disappeared in 69% of untreated patients after nine months and persisted in 31% after one year. ...	1992	1582684
normal production of neutrophil activating peptide 1/interleukin 8 in monocytes from subjects with previous yersinia triggered reactive arthritis.		1992	1586266
yersinia enterocolitica serotype 0:3-induced arthritis in mice: microbiological and histopathological information.	gross anatomical and histopathological changes in arthritic joints resulting from oral challenge with yersinia enterocolitica serotype 0:3, upon pretreatment with desferrioxamine, were always more severe than those induced by intravenous infection of immunized animals. in all the acute inflammation episodes studied, live yersiniae were isolated from the arthritic region. invariably, a heavy mixed infiltration of synovia, joint spaces and soft tissues was observed at this stage. concurrent fibrou ...	1992	1586483
plasmid-encoded outer membrane protein yada mediates specific binding of enteropathogenic yersiniae to various types of collagen.	the plasmid-encoded outer membrane protein yada of enteropathogenic yersiniae is associated with pathogenicity. recently, collagen binding of yada-positive yersiniae was reported without detailed characterization (l. emödy, j. heesemann, h. wolf-watz, m. skurnik, g. kapperud, p. o'toole, and t. wadström, j. bacteriol. 171:6674-6679, 1989). to elucidate the nature of collagen binding to yada, we used a recombinant yersinia strain expressing the cloned yada gene. direct binding of yada-positive ye ...	1992	1587583
tyrosine protein kinase inhibitors block invasin-promoted bacterial uptake by epithelial cells.	the ability to enter into (invade) mammalian cells is an essential virulence determinant of many pathogenic bacteria and intracellular parasites. these organisms are internalized by host cells upon attachment to their surface. however, the mechanisms used by intracellular parasites to induce internalization into host cells have not been defined. we found that the protein kinase inhibitor staurosporine blocks invasion by some pathogenic bacteria, including yersinia enterocolitica and yersinia pse ...	1992	1587588
intestinal symptoms and serological response in patients with complicated and uncomplicated yersinia enterocolitica infections.	clinical course, serological response and effect of antibiotic treatment were evaluated in 34 patients with uncomplicated enterocolitis and in 27 patients with reactive arthritis (rea) due to yersinia enterocolitica. patients participating in this prospective multicentre trial were randomised to treatment or no treatment with antibiotics. only 3 (11%) of the patients who later developed rea asked for medical care because of intestinal symptoms and fever and they all developed arthritis within 2 ...	1992	1589726
[adenylate cyclase. a possible factor in the pathogenicity of yersinia pestis].	biological effect of homogenous preparation of y. pestis adenylate cyclase on eucaryotic cells was studied. adenylate cyclase, added (7.5 x 10(8) g/ml) to guinea pig macrophages lowers the level of chemiluminescence to 50-70%, has an appreciable cytotoxic effect on peritoneal macrophages and suppresses phosphorylation processes of leucocyte proteins from white mice. the experimental results obtained allow to suggest y. pestis adenylate cyclase to be a pathogenic factor, contributing to the devel ...	1992	1596952
cross-reactive proteins of borrelia burgdorferi.	the specificity of serological tests for lyme borreliosis is impaired by cross-reacting antibodies. in order to select antigens for more specific tests, specific and cross-reactive proteins of borrelia burgdorferi must be identified. therefore, to analyze cross reactions of borrelia burgdorferi with other bacteria, rabbit immune sera against heterologous bacteria (borrelia hermsii, treponema pallidum, treponema phagedenis, leptospira interrogans (serogroup grippotyphosa), neisseria meningitidis, ...	1992	1597198
yersinia-triggered reactive arthritis. use of polymerase chain reaction and immunocytochemical staining in the detection of bacterial components from synovial specimens.	to investigate whether microbial dna is present in synovial specimens from patients with yersinia-triggered reactive arthritis.	1992	1599522
acute and chronic liver disease associated with yersinia enterocolitica infection: a norwegian 10-year follow-up study of 458 hospitalized patients.	during the period 1974-1983, yersinia enterocolitica infection was diagnosed in 458 hospitalized patients by antibody response or isolation of the micro-organism. a total of 54 (11.9%) patients had acute liver infection, with significantly elevated serum levels (greater than or equal to 2-fold) of bilirubin and/or enzyme levels. serious liver disease with cellular necrosis was observed in biopsy specimens from two of 12 patients examined; six had unspecific changes. the patients were followed up ...	1992	1602289
acute and chronic pancreatic disease associated with yersinia enterocolitica infection: a norwegian 10-year follow-up study of 458 hospitalized patients.	during the period 1974-1983, yersinia enterocolitica infection was diagnosed in 458 hospitalized patients by antibody response or isolation of the micro-organism. eight (1.75%) patients showed signs of acute pancreatitis with elevated serum or urine levels of amylase; two patients had acute insulin-dependent diabetes. the patients were followed up for 4-14 years (until 1987). four patients were readmitted with chronic pancreatitis, and one with acute pancreatitis. diabetes developed in two males ...	1992	1602290
involvement of membrane carbohydrates of hela cells in the e. coli hb101 (pri203) invasive pathway.	the recombinant plasmid pri203 carries a yersinia pseudotuberculosis chromosomal gene that makes e. coli k-12 hb101 strain able to synthetize an outer membrane protein, invasin, which interacts with integrin receptors of eukaryotic cells, enabling this microorganism to penetrate human cultured animal cells. in this study we evaluated the involvement of hela cell membrane structural components in the early phases of the invasive pathway of e. coli hb101 (pri203). when hela cell monolayers were tr ...	1992	1602981
[clinical data and diagnosis of yersinia enterocolitica infections; a retrospective study in friesland].		1992	1603166
antibodies against yersinia among farmers and slaughterhouse workers.	antibodies to immunoglobulins (ig) m, g, and a against yersinia enterocolitica serotypes o:3, o:5, o:8, and o:9 and yersinia pseudotuberculosis serotypes i and iii were analyzed by enzyme immunoassay of the serum samples of 161 slaughterhouse workers, 147 pig farmers, and 114 grain or berry farmers. the crude risk ratios for elevated serum antibody concentrations were calculated with the use of the grain and berry farmers as the reference population. the risk for an elevated y enterocolitica o:3 ...	1992	1604274
[comparison of yersinia enterocolitica strains isolated from swine and humans in guipuzcoa].	yersinia enterocolitica biotype 4, serotype 3 is a pathogenic bacteria for men. its most likely reservoir are pigs. we have compared 48 y. enterocolitica serotype 0:3 strains isolated from pig's tonsils samples as well as porcine-derived food with 301 y. enterocolitica serotype 0:3 isolate from human stool's samples. all strains are positive for virulence tests. yersinia enterocolitica serotype 0:3, biotype 4, phage-type viii was nearly the only single pathogenic strain isolated from both origin ...	1992	1606219
[incidence of pseudo-appendicitis syndrome caused by yersinia enterocolitica].		1992	1606234
in-vitro and in-vivo activities of beta-lactam antibiotics on yersinia enterocolitica.		1992	1607334
[regulatory properties of the rat heart adenylate cyclase in the course of toxic-infective shock caused by yersinia pestis].	influence of intravenously administered to rats murine toxin of y. pestis in the dose of i mg/ml (ld100) on the regulatory properties of heart plasma membranes adenylate cyclase (ac) has been studied during the intoxication. it has been shown that basal, fluoride,- and 5-guanylyl imidodiphosphate-stimulated ac activity remained unchanged during the intoxication. stimulation of ac by isoproterenol, glucagon and histamine did not change during the first two hours and significantly decreased after ...	1992	1611068
cytometric haematological analysis of experimental yersinia pseudotuberculosis infection at temperatures of cultivation of 25 degrees c and 37 degrees c.	the dynamics of acute lethal infection with yersinia pseudotuberculosis (serovar iii) grown at 25 degrees c and 37 degrees c, respectively, were studied in guinea-pigs. using an automated hematology analyzer, the quantity and quality of the host cellular response was determined at the primary site of infection (the peritoneal cavity) and in parallel in the blood. a much more intensive leucocytic response of monocytic type was observed in the animals infected with microorganism grown at 37 degree ...	1992	1611207
interactions between yersinia enterocolitica and the host with special reference to virulence plasmid encoded adhesion and humoral immunity.	this thesis is based on 8 publications and a review of the literature. the aim was to summarize present knowledge on molecular components of yersinia enterocolitica involved in (i) adhesion, with special reference to plasmid encoded factors and (ii) induction of antibodies in the host, and the quantitation of these two phenomena. adhesion of y. enterocolitica was quantitatively studied by methods that measured binding to cultured epithelial cells, mucosal constituents immobilized on polystyrene, ...	1992	1611921
adhesion protein yada of yersinia species mediates binding of bacteria to fibronectin.	the interaction between fibronectin and yersinia strains was studied. wild-type y. enterocolitica strains expressing the virulence-plasmid-encoded adhesion protein yada adhered strongly to fibronectin-coated coverslips, while their plasmid-cured variants expressed weaker binding. the cloned yada gene of y. enterocolitica or y. pseudotuberculosis conferred fibronectin-binding ability both to escherichia coli and to y. psuedotuberculosis strains lacking the yada protein. the yada protein did not m ...	1992	1612772
the difference in the lcrv sequences between y. pestis and y. pseudotuberculosis and its application for characterization of y. pseudotuberculosis strains.	we have sequenced the lcrgvh operon from y. pseudotuberculosis plasmid pyv995 and compared its sequence with that of y. pestis. the sequences were highly homological, however, six base pair substitutions were found in one short 14 bp region termed variable sequence. two oligonucleotides corresponding to variable sequence of y. pestis (pes-v) or y. pseudotuberculosis (ptb-v) were synthesized and were used as molecular probes in hybridization experiments with sets of y. pestis and y. pseudotubercu ...	1992	1614327
analysis of yersinia pestis chromosomal determinants pgm+ and psts associated with virulence.	the ability to absorb exogenous pigments (pgm+) has, until now, been considered an established virulence factor of yersinia pestis, the causative agent of plague. this property correlates with the sensitivity to the bacteriocin pesticin (psts). both functions are chromosomally encoded. in the present study, using hfr donors and isogenic pgm-psts and pgm-pstr mutants, these functions were shown to be determined by discrete but closely linked genes. these markers designated pgm and psn, respective ...	1992	1614328
polyarteritis associated with yersinia enterocolitica infection.	a patient developed polyarteritis, predominantly affecting the muscles, 10 days after a yersinia enterocolitica o:3 infection. immunoperoxidase staining showed yersinia enterocolitica o:3 antigen in the subendothelial layer of the blood vessels. this suggests that vasculitis should be considered as a rare manifestation of yersinia enterocolitica infection.	1992	1616339
immunoreactivity to yersinia enterocolitica antigens in patients with autoimmune thyroid disease.	recent reports have suggested that yersinia enterocolitica proteins encoded by a 72-kilobase virulence plasmid (known as release proteins and now identified as yop2-5) are antigens recognized specifically by patients with graves' disease and of potential etiological importance in this disorder. to examine this hypothesis, we evaluated immune responses to yop in patients with autoimmune thyroid disease and in normal controls. humoral responses to yersinia were assessed using western blots of crud ...	1992	1619022
reaction of macrophage disappearance as a method for the evaluation of cellular immunity in experimental plague.	the reaction of macrophage disappearance (rmd) was found to develop one day after the immunization of experimental animals with the antigen fia of y. pestis and to persist for 21 days, correlating with a pronounced protective effect of the above antigen over this period. there were differences between white mice and guinea pigs in the intensity of the rmd, particularly when immunization was carried out using an fia preparation without prolonged action. these differences are thought to reflect va ...	1992	1619289
long-term starvation survival of yersinia ruckeri at different salinities studied by microscopical and flow cytometric methods.	cultures of three strains of the fish pathogenic bacterium yersinia ruckeri survived starvation in unsupplemented water for at least 4 months. at salinities of 0 to 20/1000 there were no detectable changes in cfu during the first 3 days of starvation and only a small decrease during the following 4 months, whereas at 35/1000 salinity, the survival potential of the cultures was markedly reduced. these results suggest that y. ruckeri may survive for long periods in freshwater and brackish environm ...	1992	1622232
thrombotic thrombocytopenic purpura mimicking acute small bowel crohn's disease.	a 39 year old woman presented with a short history of bloody diarrhoea. she subsequently developed microangiopathic haemolysis, platelet consumption, and renal impairment. initial investigations suggested underlying crohn's disease of the terminal ileum complicated by sepsis and disseminated intravascular coagulation. however, after resection of a perforated caecum and terminal ileum, the diagnosis of thrombotic thrombocytopenic purpura was made. there was weak serological evidence of yersinia i ...	1992	1624173
temperature sensing in yersinia pestis: regulation of yope transcription by lcrf.	in escherichia coli, a yope::lacz fusion was found to be regulated by temperature in the presence of the cloned bamhi g fragment of yersinia pestis plasmid pcd1, which contains the lcrf locus. increasing the copy number of lcrf relative to that of the yope reporter had a negligible effect on the induction ratio (26 versus 37 degrees c) but caused large reductions in the absolute levels of yope transcription. we localized the lcrf gene by monitoring the induction phenotype of bamhi g deletion der ...	1992	1624422
molecular and functional characterization of the salmonella invasion gene inva: homology of inva to members of a new protein family.	one of the earliest steps in the pathogenic cycle of the facultative intracellular pathogen salmonella spp. is the invasion of the cells of the intestinal epithelium. we have previously identified a genetic locus, inv, that allows salmonella spp. to enter cultured epithelial cells. inva is a member of this locus, and it is the first gene of an operon consisting of at least two additional invasion genes. we have constructed strains carrying nonpolar mutations in inva and examined the individual c ...	1992	1624429
structure and regulation of the yersinia pestis yscbcdef operon.	we have investigated the physical and genetic structure and regulation of the yersinia pestis yscbcdef region, previously called lcrc. dna sequence analysis showed that this region is homologous to the corresponding part of the ysc locus of yersinia enterocolitica and suggested that the yscbcdef cistrons belong to a single operon on the low-calcium response virulence plasmid pcd1. promoter activity measurements of ysc subclones indicated that yscbcdef constitutes a suboperon of the larger ysc re ...	1992	1624469
the role of catecholamines in gram-negative sepsis.	recently obtained in vitro evidence has demonstrated that neurochemicals such as the catecholamines can dramatically increase the growth of gram-negative bacteria. depending on the catecholamine, an up to 100,000-fold increase in the in vitro growth rate of pathogenic gram-negative bacteria such as escherichia coli and yersinia enterocolitica can be observed. dramatic elevations in the levels of plasma catecholamines have been amply demonstrated to occur as part of the neurophysiological respons ...	1992	1625603
identification and molecular characterization of a salmonella typhimurium gene involved in triggering the internalization of salmonellae into cultured epithelial cells.	penetration of intestinal epithelial cells is an important step in the pathogenesis of salmonella infections. we have characterized a gene, inve, that is necessary for salmonella invasion of cultured epithelial cells. the predicted amino acid sequence of inve showed significant homology to the yersinia outer membrane protein yopn (lcre). strains of salmonella carrying mutations in inve were unable to penetrate henle-407 human intestinal cells and madin-darby canine kidney cells, although they we ...	1992	1631083
caf1r gene and its role in the regulation of capsule formation of y. pestis.	a new transcription unit of the f1 gene cluster was found. the dna sequencing revealed one long open reading frame. deletion and frame shift mutation analyses have demonstrated the importance of a corresponding gene product for the f1 antigen biosynthesis. a homology of the deduced amino acid sequence with that of arac family dna-binding regulators was shown. a potential regulatory dna region is discussed.	1992	1633857
oral immunization against cholera toxin with a live yersinia enterocolitica carrier in mice.	the 70-kb pyv plasmid of yersinia enterocolitica directs the synthesis and secretion of several virulence determinants called yops. these proteins are produced during the invasion of the host tissues and induce a strong antibody response. the yop genes are transcribed from strong promoters activated by a common transcription activator. recombinant y. enterocolitica strains expressing the b subunit of the cholera toxin were constructed from a yoph-ctxb operon fusion. integration of the gene ctxb ...	1992	1634070
oral carrier vaccines: new tricks in an old trade.		1992	1634086
yersinia enterocolitica contamination of blood products. connecticut state medical society committee on organ and tissue transfers.		1992	1638859
mxia of shigella flexneri 2a, which facilitates export of invasion plasmid antigens, encodes a homolog of the low-calcium-response protein, lcrd, of yersinia pestis.	the plasmid-encoded invasion plasmid antigen (ipa) export accessory locus of shigella flexneri 2a, mxia, was cloned, and the complete dna sequence of the gene was determined. the mixa open reading frame was found to encode a polypeptide of 74.03 kda with a pi of 5.02. a hydropathy analysis of the predicted protein revealed a hydrophilic c terminus and an extremely hydrophobic n terminus without a cleavable signal sequence but with several potential membrane-spanning regions. while a homology sea ...	1992	1639496
function of terminal ileum in patients with yersinia-triggered reactive arthritis.	in order to study the function of the intestinal epithelium in the terminal ileum, the schilling test was performed in 10 patients with yersinia-triggered reactive arthritis, in 10 patients who had recovered from yersinia enteritis without complications, and in five patients with rheumatoid arthritis treated with non-steroidal anti-inflammatory agents. the schilling test indicates absorption of vitamin b12 in the terminal ileum, i.e. the area affected by yersinia and inflamed in patients with re ...	1992	1640195
ferrioxamine uptake in yersinia enterocolitica: characterization of the receptor protein foxa.	the gene for the high-affinity outer membrane ferrioxamine receptor foxa of yersinia enterocolitica was cloned in escherichia coli k-12. a foxa mutant of yersinia could be complemented by the cloned dna fragment. the foxa encoding region was sequenced and an open reading frame encoding 710 amino acids, including a signal sequence of 26 amino acids, was deduced. the mature foxa protein consisted of 684 amino acids and had a molecular mass of 75,768 da. foxa shared 33% amino acid sequence homology ...	1992	1640832
a spontaneous yersinia pseudotuberculosis-infection in a monkey-colony.	a spontaneous yersinia pseudotuberculosis (y.p.) infection in one african green monkey (cercopithecus aethiops), and nine squirrel monkeys (saimiri sciureus), is reported. the clinical findings, laboratory results and pathological findings are presented. the digestive system was the most affected organ in the acute phase of the y.p. infection, while in the subacute and chronic phases, the alterations of the lymphatic tissues, spleen or liver were severe.	1992	1642076
[yersiniosis].		1992	1643332
epizootic of yersinia pseudotuberculosis in a wildlife park.	an epizootic attributable to yersinia pseudotuberculosis infection was confirmed in captive ruminants in a wildlife park by microbiologic and histologic findings. an epornitic of y pseudotuberculosis infection was identified at the same period in a vicinity near the ruminant deaths. yersinia pseudotuberculosis can infect human beings and cause acute enteritis and mesenteric lymphadenitis. people in contact with infected animals should use extreme caution and use good sanitary precautions to prec ...	1992	1644638
in-vitro evaluation of ampicillin/brobactam and comparison with other beta-lactam antibiotics.	the ability of brobactam to inhibit beta-lactamases and the in vitro activity of ampicillin combined with brobactam was compared with another beta-lactamase inhibitor, clavulanic acid, and other beta-lactam antibiotics. both inhibitors showed good and similar activity against staphylococcal penicillinase and most broad-spectrum beta-lactamases found in the enterobacteriaceae, whether plasmid or chromosomally mediated. both inhibitors were less active against chromosomally mediated cephalosporina ...	1991	1646779
characterization of a hemin-storage locus of yersinia pestis.	the pigmentation phenotype (pgm+) of yersinia pestis refers to temperature-dependent storage of hemin as well as expression of a number of other physiological characteristics. spontaneous mutation to a pgm- phenotype occurs via a large chromosomal deletion event and results in the inability to express the pgm+ characteristics. in this study, we have used transposon insertion mutants to define two regions of a hemin-storage (hms) locus. a clone (phms1) encompassing this locus reinstates expressio ...	1991	1649616
cloning and sequence analysis of a trans-regulatory locus required for exoenzyme s synthesis in pseudomonas aeruginosa.	exoenzyme s is an adp-ribosyltransferase enzyme distinct from exotoxin a that is synthesized and secreted by pseudomonas aeruginosa. yields of exoenzyme s are variable and depend on strain and growth conditions. since certain medium additives are required for exoenzyme s production, its regulation may be influenced by environmental stimuli. in this study, we have cloned a region that complements the exoenzyme s-deficient phenotype of strain 388 exs1::tn1, a chromosomal tn1 insertional mutation. ...	1991	1655713
nosocomial outbreaks: the centers for disease control's hospital infections program experience, 1980-1990. epidemiology branch, hospital infections program.	from january 1980 to july 1990, the hospital infections program of the centers for disease control conducted 125 on-site epidemiologic investigations of nosocomial outbreaks. seventy-seven (62%) were caused by bacterial pathogens, 11 (9%) were caused by fungi, 10 (8%) were caused by viruses, five (4%) were caused by mycobacteria, and 22 (18%) were caused by toxins or other organisms. the majority of fungi and mycobacterial outbreaks occurred since july 1985. fourteen (11%) outbreaks were device ...	1991	1656744
prevalence of antibodies to five selected zoonosis agents in monkeys.	the prevalence of antibodies against 5 zoonosis agents was determined in serum samples of 443 breeding monkeys. of the monkeys, 296 were bred or kept for a long time at r institute, and the remaining 147 were newly imported from the philippines and kept at s institute for quarantine. antibodies to simian virus 40 were highly prevalent at 89.1% among monkeys of r institute, whereas no antibody could be detected in those of s institute. antibodies to chlamydia psittaci and yersinia pseudotuberculo ...	1991	1657210
pulse field electrophoresis of the chromosome of the pathogenic yersiniae.		1991	1657518
interaction of yersinia pestis with mouse peritoneal macrophages in vitro.		1991	1657519
application of restriction endonuclease analysis and genetic probes in the epidemiology of yersinia enterocolitica infection.		1991	1657520
lcrd, a membrane-bound regulator of the yersinia pestis low-calcium response.	yersinia pestis, the etiologic agent of bubonic plague, contains a 75-kb virulence plasmid, called pcd1 in y. pestis kim. the low-ca(2+)-response genes of y. pestis regulate both bacterial growth and the expression of pcd1-encoded virulence determinants in response to temperature and the presence of ca2+ or nucleotides. this study characterizes the nucleotide sequence and protein product of the lcrd locus. an lcrd mutant, in contrast to the parent y. pestis, did not undergo growth restriction or ...	1991	1657887
[the significance of pesticin 1 for the virulence and immunogenicity of yersinia pestis].	the work deals with the study of the virulent and immunogenic properties of y. pestis strains which lost their capacity for producing pesticine 1 as the result of the insertion of a tn-like element into the 6-md plasmid responsible for this property. the "switching-off" of gene pst induced a decrease in the virulence of y. pestis injected subcutaneously into white mice and guinea pigs and had no influence on its level of immunogenicity for white mice. a suggestion was made that pesticine 1 playe ...	1991	1659072
[chromosomal resistance of plague agent to quinolones].	the nature of increasing chromosomal resistance to quinolones was studied in a model of the plague microbe. five virulent strains of the natural plague microbe (y. pestis) were used in the experiment: 363 (1/1479), 231, 2385, 2442 and 2444. the one-stage procedure for isolation of the mutants was applied. it was shown that the frequency of the one-stage mutants resistant to oxalinic acid, pefloxacin and ciprofloxacin amounted to 10(-9)-10(-11) and was 2 to 3 orders of magnitude lower than that o ...	1991	1661573
[group disease in children caused by enteroviruses and yersinia].		1991	1663297
the specificity of bacterial siderophore receptors probed by bioassays.	the ability to utilize siderophores of bacterial and fungal origin has been studied in wild-type and mutant strains of the enterobacterial genera salmonella, escherichia, shigella, moellerella, klebsiella, enterobacter, hafnia, pantoea, ewingella, tatumella, yersinia, and in the non-enterics aeromonas, pseudomonas and aureobacterium. although only a few representative strains were tested, the results show characteristic genus-specific differences in the utilization of hydroxamate and catecholate ...	1991	1663779
the prevalence of enteric pathogens in diarrhoeic thoroughbred foals in britain and ireland.	a survey of 77 normal and 326 diarrhoeic foals in britain and ireland from 1987 to 1989 revealed a significantly higher prevalence of group a rotaviruses and aeromonas hydrophila in diarrhoeic foals. the prevalence of cryptosporidia, potentially pathogenic escherichia coli, yersinia enterocolitica and clostridium perfringens was similar in normal or diarrhoeic foals. rotaviruses had a similar prevalence in all age groups of scouring foals up to three months of age, with an overall prevalence of ...	1991	1663866
use of pulsed field gel electrophoresis to size the chromosome of the bacterial fish pathogen yersinia ruckeri.	field inversion gel electrophoresis (fige) and contour-clamped homogeneous field (chef) electrophoresis were used to analyse the chromosome of yersinia ruckeri. the 8 base-pair recognition endonucleases, noti and sfii, generated less than 47 dna fragments whose size and distribution were appropriate for pulsed field separation. each isolate displayed a characteristic restriction pattern, with about 20% of bands in common. depending on the strain used, the estimated genome size for this bacterial ...	1991	1663893
in vitro activity and stability against novel beta-lactamases of investigational beta-lactams (cefepime, cefpirome, flomoxef, sce2787 and piperacillin plus tazobactam) in comparison with established compounds (cefotaxime, latamoxef and piperacillin).	the therapeutic perspectives of flomoxef, sce 2787, cefpirome, cefepime, latamoxef, cefotaxime and of piperacillin plus tazobactam were comparatively evaluated by their in vitro activity against 1119 clinical isolates of 83 bacterial species. escherichia coli, klebsiella spp. enterobacter sakazakii, proteus spp. and shigella spp. were about equally susceptible to the cephalosporins (mic90: 0.06 to 0.5 mg/l), while the mic90 for piperacillin plus tazobactam was between 2 and 16 mg/l. enterobacter ...	1991	1664418
[phenotypic features of a strain of yersinia pseudotuberculosis with the pvm82 plasmid, detected at pseudotuberculosis outbreak foci].	the phenotypic properties conferred to yersinia pseudotuberculosis cells by the genetical determinants of a 25md fragment of the plasmid pvm82 coding for the modified cellular immune response in the infected organism. the fragment was shown to determine the conjugative properties of the plasmid, the resistance of bacterial cells to a number of hydrophobic agents and cellular ability to absorb the congo red dye. the latter confirms the presence of additional structural components in the cell wall ...	1991	1664911
[changes in the myeloperoxidase and acid phosphatase activities in human peripheral blood neutrophils with the cells stimulated in vitro].	in four series of experiments human peripheral blood neutrophils were found capable of synthetizing the active forms of such enzymes as myeloperoxidase and acid phosphatase after stimulation with opsonized zymosan, and the optimum conditions for testing the synthetizing activity of neutrophils were established. the examination of 39 practically healthy donors revealed an approximate equilibrium between the synthesis of the active forms of the enzyme and its excretion from the cell after this cel ...	1991	1666206
[clinical-etiological correlations in 94 cases of acute infantile diarrhea].	the authors consider the clinical and etiological correlations in 94 children (0-36 months) with acute diarrhoea, hospitalised in 1989. they compare epidemiological data (sex, age, seasonality), clinical data (fever, vomiting, distinctive features of diarrhoea, abdominal pain) and laboratory data with the main etiological agents isolated.	1991	1666844
[yersinia enterocolitica strains of bovine origin isolated in san luis, argentina: virulence, enterotoxigenicity, and antibiotic sensitivity].	eleven yersinia enterocolitica strains isolated from bovine tongues and classified as b1 0:5 lis xz (8), b2 0:9 lis x3 (2), nontypable (1), were examined for the following virulence-associated characteristics: autoagglutination, calcium dependency, sereny test, congo red binding and presence of 40-48 mda plasmids. heat-stable enterotoxin production was also determined. antibiotic susceptibility was assayed according to the modified bauer-kirby's method. two y. enterocolitica b1 0:5 lis xz strain ...	1991	1670473
double-blind, placebo-controlled study of three-month treatment with lymecycline in reactive arthritis, with special reference to chlamydia arthritis.	we conducted a double-blind, placebo-controlled, randomized study of 3-month treatment with lymecycline, a form of tetracycline, in reactive arthritis (rea). lymecycline therapy significantly decreased the duration of the illness in patients with chlamydia trachomatis-triggered rea, but not in other rea patients. in 2 rea patients, c trachomatis was found in the throat, an uncommon locale for this organism. our results suggest that it is important to verify the triggering microbe and that it is ...	1991	1670621
detection of pathogenic yersinia enterocolitica by polymerase chain reaction.		1991	1671502
discrimination between intracellular uptake and surface adhesion of bacterial pathogens.	most bacterial pathogens initiate infectious diseases by adhering to host cells. bacterial adherence to nonphagocytic cells usually leads to extracellular colonization; however, many invasive microorganisms enter host cells after binding to the host cell surface. it is unclear why bacterial adherence can result in these two different fates for the microorganism. analyses of model systems, such as the uptake of enteropathogenic yersinia into cultured cells, indicate that the particular mammalian ...	1991	1674624
computer-assisted statistical analyses of enzyme and ribosomal dna electrophoretic polymorphism in yersinia.	the intra- and inter-species differentiation of 90 strains of yersinia belonging to six species were studied independently by computer-assisted statistical analysis of data from enzyme electrophoretic polymorphism and ribosomal dna (rdna) restriction fragment length polymorphism. two correspondence analyses (ca) demonstrated the concordance between the bacterial classification obtained from enzymatic and genomic data. this concordance was reinforced by an algorithm of the correspondence establis ...	1991	1676966
expression of the envelope antigen f1 of yersinia pestis is mediated by the product of caf1m gene having homology with the chaperone protein papd of escherichia coli.	the effective synthesis of the envelope antigen f1 of y. pestis in e. coli hb101 is mediated by the expression of the caf1m gene. this gene was sequenced, and the protein encoded was found to have a significant homology with the chaperone protein papd of uropathogenic e. coli. the data presented allow one to suppose caf1m and papd proteins perform similar functions in the biogenesis of the y. pestis capsule and e. coli p-pili, respectively.	1991	1677900
current investigations of the microbiology of yersiniae. 5th international symposium on yersinia. mt. fuji, japan, september 24-26, 1990. proceedings.		1991	1682106