Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| cutaneous and pulmonary mycosis in green anacondas (euncectes murinus). | two dead, captive green anacondas (eunectes murinus), including one male and one female, submitted for necropsy were in poor body condition, having multiple, scattered, dark red foci on the scales and mottled lungs. both snakes had severe mycotic dermatitis. in addition, the male snake had mycotic stomatitis, and the female snake had mycotic pneumonia. trichophyton sp., verticillium sp., and alternaria sp. were isolated from the dermal lesions. the pulmonary lesions were morphologically consiste ... | 2004 | 15732602 |
| synergy between cephalosporin and aminoglycoside antibiotics against providencia and proteus. | clinical isolates of providencia and proteus with relative aminoglycoside resistance were tested for susceptibility to combinations of gentamicin or tobramycin with cephalothin or cefazolin. the minimal bactericidal concentration of aminoglycoside for one-third of the strains was reduced by fourfold or more in the presence of one-fourth of the minimal bactericidal concentration of either cephalosporin. this effect was achieved by clinically attainable concentrations of cephalothin or cefazolin. | 1974 | 15825407 |
| cefoxitin and cephalothin: antimicrobial activity, human pharmacokinetics, and toxicology. | cefoxitin, a semisynthetic cephamycin, has been compared with the widely used parenteral cephalosporin, cephalothin, in terms of antibacterial activity, human pharmacokinetics, and toxicity. for both compounds, minimal inhibitory concentrations were within the therapeutic range against the 156 gram-positive cocci tested (except group d streptococci), but cephalothin was 8 to 20 times more active. regarding the 313 gram-negative organisms tested, both antibiotics were of approximately equal activ ... | 1974 | 15830475 |
| structure of the o-polysaccharide from the lipopolysaccharide of providencia stuartii o43 containing an amide of d-galacturonic acid with l-serine. | the o-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of providencia stuartii o43:h28 and studied by sugar and methylation analyses, smith degradation and 1h and 13c nmr spectroscopy, including 2d roesy, and h-detected 1h, 13c hsqc and hmbc experiments, as well as a noesy experiment in a 9:1 h2o/d2o mixture to reveal correlations for nh protons. it was found that the polysaccharide is built up of linear tetrasaccharide repeating units containing an amide of d-galac ... | 2005 | 15854614 |
| the o-polysaccharide from the lipopolysaccharide of providencia stuartii o44 contains l-quinovose, a 6-deoxy sugar rarely occurring in bacterial polysaccharides. | the o-polysaccharide (o-antigen) of providencia stuartii o44:h4 (strain 3768/51) was obtained by mild acid degradation of the lipopolysaccharide and studied by sugar and methylation analyses along with (1)h and (13)c nmr spectroscopy, including 2d (1)h,(1)h cosy, tocsy, roesy, and h-detected (1)h,(13)c hsqc, and hmqc-tocsy experiments. the o-polysaccharide was found to have a branched hexasaccharide repeating unit of the following structure: [formula: see text]. | 2005 | 15854616 |
| genetic environment and expression of the extended-spectrum beta-lactamase blaper-1 gene in gram-negative bacteria. | the genetic location of the gene coding for the expanded-spectrum beta-lactamase per-1 was analyzed in a series of gram-negative isolates. it was identified as part of a composite transposon bracketed by two novel insertion elements, ispa12 and ispa13, belonging to the is4 family that possess transposases that share 63% amino acid identity and that are chromosomally located in pseudomonas aeruginosa, providencia stuartii, and acinetobacter baumannii. on the contrary, the bla(per-1) gene was iden ... | 2005 | 15855485 |
| visualization of protoplast fusion and quantitation of recombination in fused protoplasts of auxotrophic strains of escherichia coli. | protoplast fusion has been used to combine genes from different organisms to create strains with desired properties. a recently developed variant on this approach, genome shuffling, involves generation of a genetically heterogeneous population of a single organism, followed by recursive protoplast fusion to allow recombination of mutations within the fused protoplasts. these are powerful techniques for engineering of microbial strains for desirable industrial properties. however, there is a prev ... | 2005 | 15974564 |
| oxalate-degrading providencia rettgeri isolated from human stools. | oxalate-degrading bacteria are thought to metabolize intestinal oxalate and thus decrease the urinary excretion of oxalate by reducing its intestinal absorption. | 2005 | 15985073 |
| moxifloxacin (bay 12-8039): a new methoxy quinolone antibacterial. | moxifloxacin (bay 12-8039) is a new 8 methoxy quinolone antibacterial. the mic90 values are < or = 0.25 mg/l for streptococcus pneumoniae (irrespective of penicillin susceptibility), haemophilus influenzae (beta-lactamase positive or negative), morexella catarrhalis, bordetella pertussis, legionella sp., mycoplasma pneumoniae, clamydia pneumoniae, mycobacterium tuberculosis, methicillin-sensitive staphylococcus aureus, beta-haemolytic streptococci (macrolide-sensitive or -resistant), listeria sp ... | 1999 | 15992072 |
| novel genetic structure associated with an extended-spectrum beta-lactamase blaveb gene in a providencia stuartii clinical isolate from algeria. | a ceftazidime-resistant providencia stuartii isolate from algeria harbored a ca. 160-kb conjugative plasmid that contained a truncated bla(veb-1b) gene flanked by three 135-bp repeated elements. this work gives further evidence of the worldwide spread of bla(veb) genes that are associated with genetic structures other than class 1 integrons. | 2005 | 16048993 |
| surveillance of antimicrobial resistance in bulgaria--a synopsis from bulstar 2003. | we introduce bulgarian surveillance tracking antimicrobial resistance (bulstar) and make the first report on surveillance data for 2003. this longitudinal surveillance programme monitors the isolation and antimicrobial susceptibility of all clinically significant microorganisms isolated from blood cultures, cerebrospinal fluid, upper and lower respiratory tract, urine and wound samples in the participating microbiology laboratories. twenty eight public, 45 hospital and 6 private laboratories fro ... | 2005 | 16077213 |
| enteric fever-like illness caused by infection with citrobacter amalonaticus. | 'enteric fever' is a potentially fatal, severe systemic disease, which is encountered worldwide. traditionally, enteric fever refers to a bacteremic illness caused by members of certain salmonella serotypes, notably: salmonella typhi, a gram-negative bacterium, and to a lesser extent, salmonella paratyphi a, b and c. in addition, other non-salmonella organisms may produce a syndrome clinically indistinguishable from "enteric fever". brucella sp., campylobacter sp., edwardsiella tarda, enterobact ... | 2005 | 16083226 |
| characterization of the type iii restriction endonuclease pstii from providencia stuartii. | a new type iii restriction endonuclease designated pstii has been purified from providencia stuartii. pstii recognizes the hexanucleotide sequence 5'-ctgatg(n)(25-26/27-28)-3'. endonuclease activity requires a substrate with two copies of the recognition site in head-to-head repeat and is dependent on a low level of atp hydrolysis ( approximately 40 atp/site/min). cleavage occurs at just one of the two sites and results in a staggered cut 25-26 nt downstream of the top strand sequence to generat ... | 2005 | 16120967 |
| expression and purification of penicillin g acylase enzymes from four different micro-organisms, and a comparative evaluation of their synthesis/hydrolysis ratios for cephalexin. | several genes for the enzyme penicillin g acylase, as isolated from four different micro-organisms (alcaligenes facaelis, escherichia coli, kluyvera cryocrescens or providencia rettgeri) were modified at their carboxy-termini to include his-tag fusions, then were expressed from the plasmid pet-24a(+) in e. coli jm109(de3) cells. all fusion proteins were next purified to homogeneity in a single step by agar-based co-ida chromatography, and were then evaluated as catalysts for the synthesis of cep ... | 2006 | 16139515 |
| [surveillance of susceptibility of clinical isolates to cefmetazole between 2000 and 2003]. | for the post-marketing surveillance of cefmetazole (cmz, cefmetazon), mics of injectable beta-lactam antibacterials including cmz against clinical isolates from 15 medical institutions all over japan are measured yearly and the incidence rates of resistance in various species are also evaluated. in the first surveillance from june 2000 to march 2001, 574 isolates of 13 species were tested, 548 isolates of the same 13 species were tested in the second surveillance from april 2001 to march 2002, a ... | 2005 | 16161756 |
| phylogeny of the enterobacteriaceae based on genes encoding elongation factor tu and f-atpase beta-subunit. | the phylogeny of enterobacterial species commonly found in clinical samples was analysed by comparing partial sequences of their elongation factor tu gene (tuf) and of their f-atpase beta-subunit gene (atpd). an 884 bp fragment for tuf and an 884 or 871 bp fragment for atpd were sequenced for 96 strains representing 78 species from 31 enterobacterial genera. the atpd sequence analysis exhibited an indel specific to pantoea and tatumella species, showing, for the first time, a tight phylogenetic ... | 2005 | 16166704 |
| [providencia alcalifaciens as a newly recognized cause of food-borne disease]. | 2005 | 16180691 | |
| metallo-beta-lactamase imp-1 in providencia rettgeri from two different hospitals in japan. | in 2002, 495 indole-positive proteae strains were isolated from patients at 60 hospitals in japan. nine indole-positive proteae strains had reduced susceptibility to imipenem (mic > or = 8 microg ml(-1)) and were identified as providencia rettgeri by bd phoenix. eight of the nine prov. rettgeri isolates were confirmed as metallo-beta-lactamase producers by the double-disc synergy test. all the metallo-beta-lactamases were classified as imp-1 by pcr and dna sequence analysis. these bla(imp-1) gen ... | 2005 | 16192438 |
| importance of providencia species as a major cause of travellers' diarrhoea. | in this study the importance of providencia species as a cause of travellers' diarrhoea was examined using a selective medium developed by the authors. providencia species could easily be distinguished from other enteric pathogens by the colour of the colonies obtained. nine strains of providencia alcalifaciens, nine of providencia rettgeri and five of providencia stuartii were isolated from 130 specimens, representing a surprisingly high incidence of infection compared with other pathogens isol ... | 2005 | 16192440 |
| acid phosphatase/phosphotransferases from enteric bacteria. | we have investigated the enzymatic phosphorylation of nucleosides and found that morganella morganii phoc acid phosphatase exhibits regioselective pyrophosphate (pp(i))-nucleoside phosphotransferase activity. in this study, we isolated genes encoding an acid phosphatase with regioselective phosphotransferase activity (ap/ptase) from providencia stuartii, enterobacter aerogenes, escherichia blattae and klebsiella planticola, and compared the primary structures and enzymatic characteristics of the ... | 2001 | 16233057 |
| [study of real-time pcr assays for rapid detection of food-borne pathogens]. | a duplex real-time sybr green lightcycler pcr (lc-pcr) assay with dna extraction using qiaamp dna stool minikit was evaluated for detection of 8 of 19 species of food-borne pathogens, including plesiomonas shigelloides, providencia alcalifaciens, in five stool specimens. the time frame was within 2h or less. the protocol used the same lc-pcr with 22 pairs of specific primers. the rapid amplification and reliable detection of specific genes were determined by this lc-pcr assay from 10 cases of fo ... | 2005 | 16248373 |
| high-level production and covalent immobilization of providencia rettgeri penicillin g acylase (pac) from recombinant pichia pastoris for the development of a novel and stable biocatalyst of industrial applicability. | a complete, integrated process for the production of an innovative formulation of penicillin g acylase from providencia rettgeri(rpac(p.rett))of industrial applicability is reported. in order to improve the yield of rpac, the clone ln5.5, carrying four copies of pac gene integrated into the genome of pichia pastoris, was constructed. the proteinase activity of the recombinant strain was reduced by knockout of the pep4 gene encoding for proteinase a, resulting in an increased rpac(p.rett) activit ... | 2006 | 16259000 |
| structures of the o-polysaccharides and classification of proteus genomospecies 4, 5 and 6 into respective proteus serogroups. | an acidic branched o-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide (lps) of proteus genomospecies 4 and studied by sugar and methylation analyses along with 1h and 13c nmr spectroscopy, including 2d cosy, tocsy, roesy and h-detected 1h, 13c hsqc experiments. the following structure of the pentasaccharide repeating unit of the o-polysaccharide was established, which is unique among proteus polysaccharide structures: [structure: see text] where qui3nac stands for 3 ... | 2005 | 16262693 |
| microbiological evaluation of bottled non-carbonated ("still") water from domestic brands in greece. | the microbiological quality of 1,527 samples of bottled non-carbonated ('still') mineral water, purchased from retail outlets and derived from 10 manufacturing companies in greece, was investigated during the period 1995-2003. applying the membrane filter technique, the aliquots of water samples (250 ml) were analyzed for the presence and enumeration of total coliforms, escherichia coli, enterococcus spp. and pseudomonas aeruginosa. also, aerobic bacteria were counted as heterotrophic plate coun ... | 2006 | 16271413 |
| improved susceptibilities to cinoxacin and other antimicrobial agents of some providencia species re-identified on the basis of fermentation of polyhydric alcohols. | 1989 | 16312291 | |
| effect of triclosan on the development of bacterial biofilms by urinary tract pathogens on urinary catheters. | to examine (i) the effect of triclosan on the formation of catheter biofilms by urinary tract pathogens and (ii) the diffusion of triclosan through the retention balloons of urinary catheters. | 2006 | 16339844 |
| enterobacteria in feedlot waste and runoff. | samples of beef cattle feedlot waste (flw), runoff from the pens, and water from a large drainage ditch at the feedlot were examined for enterobacteriaceae. the drainage ditch receives the runoff but contains primarily subsurface drainage from fields on which flw is spread for disposal. planting and enrichment techniques with seven different media were used to isolate 553 cultures of enterobacteria. flw contains about 50 million enterobacteria/g dry weight. more than 90% of these were escherichi ... | 1972 | 16349932 |
| identification of escherichia coli o157:h7 meat processing indicators for fresh meat through comparison of the effects of selected antimicrobial interventions. | fresh meat products can become contaminated with the pathogen escherichia coli o157:h7 during the slaughter process; therefore, an e. coli o157:h7 indicator to verify the effectiveness of process controls in slaughter establishments would be extremely useful. the hides of 20 beef cattle were sampled, and 113 bacterial isolates were obtained. thirteen of these isolates representing four genera, escherichia, enterobacter, providencia, and serratia, were selected based on growth and biochemical cha ... | 2005 | 16355829 |
| pharmacologic characteristics of prulifloxacin. | prulifloxacin, the prodrug of ulifloxacin, is a broad-spectrum oral fluoroquinolone antibacterial agent. after absorption, prulifloxacin is metabolized by esterases to ulifloxacin. the drug has a long elimination half-life, allowing once-daily administration. in vitro, ulifloxacin is generally more active than other fluoroquinolones against a variety of clinical isolates of gram-negative bacteria, including community and nosocomial isolates of escherichia coli, klebsiella spp., proteus, providen ... | 2006 | 16360331 |
| screening of antibiotics resistance to enterobacteriaceae, pseudomonas aeruginosa, and acinetobacter baumannii by an advanced expert system. | the vitek2 advanced expert system (aes) gives information about the antibiotics-resistance mechanisms based on the biological validation derived from the vitek2 susceptibility result. in this study, we investigated whether or not this system correctly categorized the beta-lactamase resistance mechanism data derived from the vitek2 susceptibility result using the testing card, ast-n025, with enterobacteriaceae, pseudomonas aeruginosa, and acinetobacter baumannii. we used 131 strains, and their ph ... | 2005 | 16369735 |
| isolation of salmonella bacteriophages from swine effluent lagoons. | bacteriophages (phages) associated with salmonella were collected from nine swine manure lagoons in mississippi. phages were isolated by an enrichment protocol or directly from effluent. for enrichment, chloroform-treated samples were filtered (0.22 mum) and selectively enriched by adding a cocktail of salmonella strains in trypticase soy broth. after overnight incubation at 35 degrees c, chloroform was added and samples stored at 5 degrees c. enriched samples were tested by double agar layer (d ... | 2006 | 16455853 |
| clonal occurrences of multidrug-resistant gram-negative bacilli: report from the meropenem yearly susceptibility test information collection surveillance program in the united states (2004). | the meropenem yearly susceptibility test information collection program is a global, longitudinal resistance surveillance network of more than 100 medical centers worldwide monitoring the susceptibility of bacterial pathogens to carbapenems and other broad-spectrum agents. between 1999 and 2004, a total of 10-16 us medical centers referred up to 200 nonduplicate isolates from clinical infections to a central processing laboratory. over this 6-year period, the antimicrobial activity of 12 broad-s ... | 2006 | 16466890 |
| the structure of the o-polysaccharide from the lipopolysaccharide of providencia alcalifaciens o30. | the o-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of providencia alcalifaciens o30. studies by sugar and methylation analyses along with (1)h and (13)c nmr spectroscopy, including two-dimensional (1)h,(1)h cosy, tocsy, roesy, and h-detected (1)h,(13)c hsqc, hmbc, and hmqc-tocsy experiments, showed that the polysaccharide has a linear pentasaccharide repeating unit of the following structure: | 2006 | 16472790 |
| providencia vermicola sp. nov., isolated from infective juveniles of the entomopathogenic nematode steinernema thermophilum. | in the course of isolating bacteria from infective juveniles of the entomopathogenic nematode steinernema thermophilum ganguly & singh, 2000, three isolates were obtained (op1t, op29 and vs3). on the basis of 16s rrna gene sequence analysis and riboprint patterns, these three strains were identical to each other but distinct from the type strains of the five recognized species of the genus providencia. based on biochemical and genomic analysis and supported by the low (<35 %) dna-dna relatedness ... | 2006 | 16514040 |
| optimization of ferrioxamine e concentration as effective supplementation for selective isolation of salmonella enteritidis in egg white. | utilization of ferrioxamine e (fe) as a sole source of iron distinguishes salmonella from a number of related species, including escherichia coli. fe is not able to serve as a source of iron for e. coli or the proteus-providencia-morganella group. this confers a selective advantage on salmonella enteritidis in egg white supplemented with fe. the optimum concentration of fe that promoted a selective advantage for salmonella in egg white was determined. four supplementation concentrations were eva ... | 2006 | 16541696 |
| isolation of enterobacter sakazakii from stable flies, stomoxys calcitrans l. (diptera: muscidae). | enterobacter sakazakii is an opportunistic foodborne pathogen that causes meningitis, enterocolitis, and sepsis, primarily in immunocompromised infants. previously, it was suggested that stable flies, stomoxys calcitrans, were a vector or reservoir of this pathogen. in our study, by means of a culturing approach combined with 16s rdna pcr-restriction fragment length polymorphism genotyping and sequencing, we screened 928 individual stable flies collected in kansas and florida. two stable flies ( ... | 2006 | 16541702 |
| functional characterization of escherichia coli glpg and additional rhomboid proteins using an aara mutant of providencia stuartii. | the providencia stuartii aara protein is a member of the rhomboid family of intramembrane serine proteases and required for the production of an extracellular signaling molecule that regulates cellular functions including peptidoglycan acetylation, methionine transport, and cysteine biosynthesis. additional aara-dependent phenotypes include (i) loss of an extracellular yellow pigment, (ii) inability to grow on macconkey agar, and (iii) abnormal cell division. since these phenotypes are easily as ... | 2006 | 16621838 |
| structure of the o-polysaccharide from the lipopolysaccharide of providencia alcalifaciens o29. | the o-polysaccharide was obtained by a mild acid degradation of the lipopolysaccharide of providencia alcalifaciens o29. structural studies were performed using sugar and methylation analyses along with 1h and 13c nmr spectroscopy, including two-dimensional 1h, 1h cosy, tocsy, roesy, h-detected 1h, 13c hsqc and hmbc experiments. on the basis of the data obtained, the following structure of the branched tetrasaccharide repeating unit of the o-polysaccharide was established: [structure: see text]. | 2006 | 16630597 |
| trends in production of extended-spectrum beta-lactamases among enterobacteria of medical interest: report of the second italian nationwide survey. | results of a 2003 survey carried out in italy to evaluate the prevalence of extended-spectrum beta-lactamase (esbl)-producing enterobacteria are presented. eleven italian microbiology laboratories investigated 9,076 consecutive nonreplicate isolates (inpatients, 6,850; outpatients, 2,226). esbl screening was performed by mic data analysis. confirmation was obtained using the double-disk synergy test and the combination disk test based on clsi methodology. esbl determinants were investigated by c ... | 2006 | 16672390 |
| antimicrobial resistance of providencia spp. isolated from animal manure. | providencia spp. are gram negative bacteria, belonging to the family enterobacteriaceae and are responsible for gastrointestinal disorders both in humans and animals. we report on the isolation of multi-drug resistant providencia spp. (n=4) from animal manure. the isolates were found to be resistant to clindamycin and to tetracycline, as well as to the macrolide and sulphonamide groups of antibiotics. | 2006 | 16772147 |
| ocular infections caused by providencia rettgeri. | to report a series of patients with ocular infections caused by the rarely described gram-negative bacterium, providencia rettgeri. | 2006 | 16797710 |
| the structure of the o-polysaccharide from the lipopolysaccharide of providencia alcalifaciens o36 containing 3-deoxy-d-manno-oct-2-ulosonic acid. | an oligosaccharide that corresponds to the repeating unit of the o-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of providencia alcalifaciens o36. structural studies of the oligosaccharide and o-deacylated lipopolysaccharide were performed using sugar and methylation analyses along with (1)h and (13)c nmr spectroscopy, including 2d (1)h,(1)h cosy, tocsy, roesy, and h-detected (1)h,(13)c hsqc and hmbc experiments. it was found that the o-polysaccharide is built up ... | 2007 | 16815342 |
| [low-intensity laser radiation in preoperative preparation of patients with benign prostatic hyperplasia]. | low-intensity laser therapy administered in the form of intravenous blood irradiation, transrectal and transurethral prostatic irradiation and their combination as preoperative preparation and correction of immunity disturbances in patients with benign prostatic hyperplasia (bph) were studied. the response to the treatment was evaluated by positive changes in the immune status and bacterial contamination of the urine and prostatic tissue. conventional preoperative preparation (uroantiseptics, an ... | 2000 | 16856453 |
| genetic variation in drosophila melanogaster resistance to infection: a comparison across bacteria. | insects use a generalized immune response to combat bacterial infection. we have previously noted that natural populations of d. melanogaster harbor substantial genetic variation for antibacterial immunocompetence and that much of this variation can be mapped to genes that are known to play direct roles in immunity. it was not known, however, whether the phenotypic effects of variation in these genes are general across the range of potentially infectious bacteria. to address this question, we ha ... | 2006 | 16888344 |
| [endocytosymbiosis of microorganisms in mammal eukaryotic cells and factors of its regulation]. | morphofunctional equivalents of the process of long-term intracellular prokaryotes--eukaryotes interaction were studied by light and electron microscopy. the mechanisms for adaptation, elaborated in the course of evolution of bacteria-host interaction, were analysed on the ultrastructural level. a concept on the role of hypothalamic nonapeptides, as factors of regulation of intracellular persistence and symbiosis of prokaryotes, is discussed. | 2006 | 16941870 |
| structure of the o-antigen of providencia stuartii o20, a new polysaccharide containing 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-d-galacto-non-2-ulosonic acid. | the o-polysaccharide chain of the lipopolysaccharide (lps) of providencia stuartii o20 was found to contain d-glucuronic acid, n-acetyl-d-glucosamine, and a rarely occurring higher sugar 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-d-galacto-non-2-ulosonic acid (di-n-acetyl-8-epilegionaminic acid, 8eleg5ac7ac). degradation of the lps with dilute acetic acid caused depolymerization of the polysaccharide chain by the ketosidic linkage to give a tetrasaccharide corresponding to the repeating unit o ... | 2007 | 16949059 |
| enterobacteria producing extended-spectrum beta-lactamases and imp-1 metallo-beta-lactamases isolated from brazilian hospitals. | 2006 | 17030928 | |
| rapid separation and concentration of food-borne pathogens in food samples prior to quantification by viable-cell counting and real-time pcr. | buoyant density gradient centrifugation has been used to separate bacteria from complex food matrices, as well as to remove compounds that inhibit rapid detection methods, such as pcr, and to prevent false-positive results due to dna originating from dead cells. applying a principle of buoyant density gradient centrifugation, we developed a method for rapid separation and concentration following filtration and low- and high-speed centrifugation, as well as flotation and sedimentation buoyant den ... | 2007 | 17056684 |
| diverse class 2 integrons in bacteria from beef cattle sources. | the purpose of this study was to determine the diversity of class 2 integrons in bacteria isolated from beef cattle sources. | 2006 | 17065187 |
| evaluation of significance of bacteria in larval development of cochliomyia macellaria (diptera: calliphoridae). | bacteria were isolated and identified from the digestive tract of the secondary screwworm, cochliomyia macellaria (f.) (diptera: calliphoridae), and their role in the larval development of this insect was assessed in laboratory bioassays. the analysis of 16s rdna sequences revealed that the bacterial isolates represented four species: providencia sp., escherichia coli o157:h7 (escherich), enterococcus faecalis (orla-jensen), and ochrobactrum sp. (holmes). developmental assays demonstrated that c ... | 2006 | 17162944 |
| structure of the o-polysaccharide and serological cross-reactivity of the lipopolysaccharide of providencia alcalifaciens o32 containing n-acetylisomuramic acid. | the o-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of providencia alcalifaciens o32 and studied by sugar and methylation analyses, solvolysis with triflic acid, 1h and 13c nmr spectroscopy, including two-dimensional 1h,1h cosy, tocsy, roesy, h-detected 1h,13c hsqc and hmbc experiments. it was found that the polysaccharide has a branched tetrasaccharide repeating unit containing 2-acetamido-3-o-[(s)-1-carboxyethyl]-2-deoxy-d-glucose (d-glcnac3slac, n-acetylisomur ... | 2007 | 17182016 |
| accelerated detection of extended-spectrum beta-lactamases in clinical isolates of enterobacteriaceae. | a prospective study is carried out to evaluate the performance of a protocol for the accelerated detection of extended-spectrum beta-lactamases (esbls) in clinical isolates of escherichia coli, klebsiella pneumoniae and other gram-negative bacteria. a modified double-disc test (mddt) is incorporated in a gram-negative template for routine susceptibility testing. the mddt identified accurately esbls in all isolates subsequently confirmed as esbl-producers by the standard clinical laboratory stand ... | 2006 | 17201201 |
| [short communication: prevalence of extended spectrum beta-lactamases in gram negative rods: data of 2001-2004 period]. | this study was undertaken to determine the prevalence of extended spectrum beta-lactamase (esbl) production by gram negative rods (gnrs) isolated from various clinical specimens (3.240 urine, 336 pus, 277 feces, 83 blood, 38 sputum) sent to our hospital laboratory, between 2001-2004. of isolated bacteria 71.4% were identified as escherichia coli, 11.1% were klebsiella spp, 4.2% were salmonella spp, 3.7% were pseudomonas spp, 3.5% were proteus spp, 2.9% were shigella spp, 2.3% were enterobacter s ... | 2006 | 17205693 |
| rhomboid protease aara mediates quorum-sensing in providencia stuartii by activating tata of the twin-arginine translocase. | the providencia stuartii aara protein is a member of the rhomboid family of intramembrane serine proteases and is required for the production of an unknown quorum-sensing molecule. in a screen to identify rhomboid-encoding genes from proteus mirabilis, tata was identified as a multicopy suppressor and restored extracellular signal production as well as complementing all other phenotypes of a prov. stuartii aara mutant. tata is a component of the twin-arginine translocase (tat) protein secretion ... | 2007 | 17215357 |
| mosaic structure of p1658/97, a 125-kilobase plasmid harboring an active amplicon with the extended-spectrum beta-lactamase gene blashv-5. | escherichia coli isolates recovered from patients during a clonal outbreak in a warsaw, poland, hospital in 1997 produced different levels of an extended-spectrum beta-lactamase (esbl) of the shv type. the beta-lactamase hyperproduction correlated with the multiplication of esbl gene copies within a plasmid. here, we present the complete nucleotide sequence of plasmid p1658/97 carried by the isolates recovered during the outbreak. the plasmid is 125,491 bp and shows a mosaic structure in which a ... | 2007 | 17220406 |
| [pyrazinamidase activity of bacteria from enterobacteriaceae family as characteristic for taxonomy]. | pyrazinamidase activity in 330 strains of bacteria from enterobacteriaceae family (14 genus, 27 species) has been assessed. pyrazinamidase activity detected in species from following genuses: citrobacter, escherichia, klebsiella, kluyvera, morganella, providencia, raourtella, salmonella, shigella, and also in proteus mirabilis, and nonpathogenic serovars of yersinia enterocolitica, y. frederiksenii. pirasinamidase was absent in serratia (s. marcescens, s. liguefaciens), hafnia alvei, p. vulgaris ... | 2006 | 17297889 |
| new structure for the o-polysaccharide of providencia alcalifaciens o27 and revised structure for the o-polysaccharide of providencia stuartii o43. | the o-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide from providencia alcalifaciens o27 and studied by sugar and methylation analyses along with (1)h and (13)c nmr spectroscopy, including 2d (1)h,(1)h cosy, tocsy, roesy, h-detected (1)h,(13)c hsqc, and hmbc experiments. it was found that the polysaccharide is built up of linear partially o-acetylated tetrasaccharide repeating units and has the following structure: [structure: see text] where qui4nfo stands for 4-f ... | 2007 | 17368583 |
| [evaluation of vitek 2 performance for identifying extended spectrum beta-lactamases in enterobacteriaceae "other than escherichia coli, proteus mirabilis and klebsiella spp"]. | production of beta-lactamases is the main resistance mechanism of gram-negative bacteria against beta-lactam antibiotics. extended spectrum beta-lactamases (esbls) have the ability to hydrolyze a broader spectrum of beta-lactam drugs. hence rapid, accurate detection of this resistance mechanism is extremely important to guide proper patient antimicrobial therapy. these enzymes are most commonly produced by klebsiella spp. and escherichia coli, but may also occur widely in other gram-negative bac ... | 2006 | 17380089 |
| population-based laboratory surveillance for tribe proteeae isolates in a large canadian health region. | the tribe proteeae comprises the genera proteus, morganella and providencia. few studies have specifically investigated the epidemiology of infections caused by the proteeae, and none has been conducted in a large non-selected population. the present study was a population-based laboratory surveillance in the calgary health region (population 1.2 million), canada during 2000-2005 that aimed to define the incidence, demographical risk-factors for acquisition and antimicrobial susceptibilities of ... | 2007 | 17403132 |
| [susceptibility of clinical isolates from primary care clinics to oral antibacterial agents]. | the antimicrobial susceptibility of clinical isolates from specimens of patients in primary care clinics in 2005 was investigated by determining the minimum inhibitory concentrations of oral antibacterial agents. the numbers of test strains were 550 for gram-positive aerobes, 700 for gram-negative aerobes, and 150 for anaerobes. cefcapene (cfpn), cefditoren (cdtr), and cefteram (cftm) showed the most potent activities against staphylococcus spp. and streptococcus spp. among the cephems tested an ... | 2007 | 17436860 |
| prevalence of community-occurring extended spectrum beta-lactamase-producing enterobacteriaceae in brazil. | the occurrence of extended-spectrum-beta-lactamase (esbl)-producing strains in the community was investigated in a private laboratory located in juiz de fora, brazil. all enterobacterial isolates analysed were collected from urine of human patients between the years 2000 and 2002. esbl production was confirmed by double disk screening, combination disk method, and etest esbl strip. the isoelectric point of each beta-lactamase was determined in the crude extracts from each isolate. detection of e ... | 2007 | 17457648 |
| providencia stuartii with vim-1 metallo-beta-lactamase. | 2007 | 17517828 | |
| [pathogenicity factors of opportunistic enterobacteria and its role in development of diarrhea]. | the data of pathogenicity factors of opportunistic enterobacteria, including klebsiella, enterobacter, citrobacter, proteus, providencia and hafnia species are submitted. the genetic control and a role of pathogenicity factors of opportunistic enterobacteria in development of diarrhea syndrome are presented. data about adhesins, hemolysins, cytotoxic necrotizing factors and bacterial modulins are described. the characteristic of cytotonic and cytotoxic enterotoxins, including lt, st, shiga-like ... | 2007 | 17523440 |
| providencia stuartii infection in a dog with severe skin ulceration and cellulitis. | the aim of this report is to present a case of severe skin ulceration and cellulitis in a seven year old mongrel dog following cystitis. the providencia stuartii strain was the only isolate from skin lesions. this bacterium is a gram-negative rod belonging to the family of enterobacteriaceae. to the best of our knowledge, even though there are some reports in the literature concerning animal infections with providencia species, there have been no cases of cellulitis in which this pathogen is inv ... | 2007 | 17547643 |
| structure of a colitose-containing o-polysaccharide from the lipopolysaccharide of providencia alcalifaciens o6. | the o-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of providencia alcalifaciens o6 and studied by sugar and methylation analysis, selective hydrolytic removal of 3,6-dideoxy-l-xylo-hexose (colitose, col), (1)h and (13)c nmr spectroscopy, including 2d (1)h,(1)h cosy, tocsy, roesy and h-detected (1)h,(13)c hsqc and hmbc experiments. the polysaccharide was found to have a branched pentasaccharide repeating unit with the following structure: [see text] remarkably, t ... | 2007 | 17559819 |
| selection of restriction endonucleases using artificial cells. | we describe in this article an in vitro system for the selection of restriction endonucleases using artificial cells. the artificial cells are generated in the form of a water-in-oil emulsion by in vitro compartmentalization. each aqueous compartment contains a reconstituted transcription/translation mix along with the dispersed dna templates. in the compartments containing endonuclease genes, an endonuclease expressed in vitro cleaves its own dna template adjacent to the gene, leaving a sticky ... | 2007 | 17567609 |
| cultivable bacterial diversity of alkaline lonar lake, india. | aerobic, alkaliphilic bacteria were isolated and characterized from water and sediment samples collected in the winter season, january 2002 from alkaline lonar lake, india, having ph 10.5. the total number of microorganisms in the sediment and water samples was found to be 10(2)-10(6) cfu g(-1) and 10(2)-10(4) cfu ml(-1), respectively. one hundred and ninety-six strains were isolated using different enrichment media. to study the bacterial diversity of lonar lake and to select the bacterial stra ... | 2008 | 17604989 |
| multidrug-resistant providencia isolates carrying blaper-1, blavim-2, and arma. | during may to july 2004, three strains of providencia spp. with multidrug-resistance (mdr) were isolated from urinary specimen of three patients hospitalized with a same hospital room. by pcr analysis, all three strains have been found to carry both vim-2 type metallo-beta-lactamase gene and per-1 type extendedspectrum beta-lactamase gene. one out of three strains carried additional resistance gene, arma, 16s rrna methylase gene responsible for high level resistance to aminoglycosides. to our kn ... | 2007 | 17618235 |
| bluish discolouration of urine drainage tube and bag in a female patient with spina bifida, paraplegia, and suprapubic cystostomy. | we present a female patient with spina bifida, paraplegia, suprapubic cystostomy, and chronic constipation, who became anxious when she noticed a bluish discolouration of her urine drainage system. urine microbiology revealed growth of providencia stuartii and staphylococcus aureus. there were no systemic features of infection and, therefore, antibiotics were not prescribed for asymptomatic bacteriuria. this patient was advised to change the urine bag every day, and was prescribed senna to facil ... | 2007 | 17619789 |
| [purple urine bag syndrome: a case report]. | the authors report a case of dark purple urine in a woman with bowel obstruction and bilateral percutaneous nephrostomy urinary diversion for 30 years. this colour was due to the presence of high urinary concentrations of 3-indoxyl sulphate due to the enzymatic activity of providencia rettgeri. a favourable course was observed in response to antibiotics. | 2007 | 17634003 |
| [tuberculosis outbreak in a school]. | to demonstrate the importance of preventive measures when a case of tuberculosis is detected, identify the causes that favored a tuberculosis outbreak in a school and determine the efficiency of obtaining induced sputum samples. | 2007 | 17663901 |
| [the antibacterial activity of fluoroquinolones against gram-negative bacteria isolated from gifu prefecture (2005)]. | we investigated the susceptibility to 6 fluoroquinolones against 433 strains of gram-negative bacteria isolated from 6 medical facilities in gifu prefecture between january and september in 2005, determined by the agar dilution methods in according with the japan society of chemotherapy. we also investigated the correlation between the degree of resistance to fluoroquinolones and the amino acid substitutions in the quinolone resistance-determining region (qrdr). the tested clinical isolates were ... | 2007 | 17867488 |
| the biofilm environment offers a possible condition for inducing the competency of dna recipient cells through nutritional starvation. | transformation phenomena occurring under conditions mimicking the biofilm environment were investigated using escherichia coli im302 (as dna recipient cells) and providencia sp. ww2 (as surrounding cells in the biofilm model). in the case of planktonic im302 cells kept at 25 degrees c, the transformation took place exclusively in the absence of organic nutrients (cod = 0), and was not substantially observed in the range of cod = 30-1500 mg o2/l. on the other hand, in the case of biofilm im302 ce ... | 2007 | 17927052 |
| demonstration and characterization of manganese superoxide dismutase of providencia alcalifaciens. | superoxide dismutases convert superoxide anions to molecular oxygen and hydrogen peroxide. these enzymes constitute one of the major defense mechanisms of cells against oxidative stress and play a role in the pathogenesis of certain invasive bacteria. in this study, we reported for the first time here that providencia alcalifaciens, a member of the family enterobacteriaceae, produces a superoxide dismutase (sod) as a major protein in culture supernatants. this protein was purified by a series of ... | 2007 | 17951985 |
| species interactions in mixed-community crystalline biofilms on urinary catheters. | previous experimental investigations of the crystalline biofilms that colonize and block urinary catheters have focussed on their formation by pure cultures of proteus mirabilis. in the urine of patients undergoing long-term catheterization, p. mirabilis is commonly found in mixed communities with other urinary tract pathogens. little is known about the effect that the other species have on the rate at which p. mirabilis encrusts catheters. in the present study, a set of data on the nature of th ... | 2007 | 17965358 |
| multiple antimicrobial resistance in enterobacteriaceae isolates from pristine freshwater. | a freshwater enterobacterial population (n = 111) was studied for antimicrobial and mercury resistance patterns, and for its possible association with biotic and abiotic factors in that environment. conventional biochemical tests identified klebsiella sp, morganella sp, serratia sp, escherichia sp, enterobacter sp, edwarsiella sp, proteus sp, citrobacter sp, providencia sp, and kluyvera sp. there was no correlation between antimicrobial resistance patterns of isolates and bacterial genera, but r ... | 2007 | 17985304 |
| identification of two entomopathogenic bacteria from a nematode pathogenic to the oriental beetle, blitopertha orientalis. | a pathogenic nematode, butlerius sp., was isolated from oriental beetle, blitopertha orientalis. the infective juveniles exhibited dose- as well as time-dependent entomopathogenicity on the larvae of b. orientalis. two bacterial species, providencia vermicola (kacc 91278) and flavobacterium sp. (kacc 91279), were isolated from the infective juveniles and identified. p. vermicola outnumbered flavobacterium sp. in the nematode host, in which the colony density of p. vermicola was found to be 21 ti ... | 2007 | 18050915 |
| susceptibility of community gram-negative urinary tract isolates to mecillinam and other oral agents. | to determine the susceptibility of community outpatient gram-negative urinary tract isolates to mecillinam and other commonly used oral agents. | 2001 | 18159352 |
| providencia alcalifaciens as the presumptive cause of diarrhoea in dog. | a case of diarrhoea in a four-month-old golden retriever is described. on the basis of anamnesis and bacteriological examination, the diagnosis of bacterial enteritis due to providencia alcalifaciens was reached. although there are contradictory opinions about the role of this organism as the enteric pathogen, it seems that providencia alcalifaciens should also be taken into consideration in the routine bacteriological diagnostics of diarrhoea in dogs. | 2007 | 18198546 |
| evaluation of different laboratory tests for the detection of metallo-beta-lactamase production in enterobacteriaceae. | clinical isolates of klebsiella pneumoniae (91), escherichia coli (49), enterobacter spp. (27), proteus mirabilis (17), citrobacter freundii (2), providencia stuartii (3) and serratia spp. (5), with various mics of imipenem, were examined for production of metallo-beta-lactamases (mbls) with different phenotypic laboratory tests that have been previously published to detect mbls in pseudomonas aeruginosa and acinetobacter spp. | 2008 | 18222954 |
| [abscess of the posterior cranial fossa. report of 4 cases]. | the posterior cerebral fossa is an uncommon location for cerebral abscess. in most cases diagnosis is made at the encapsulation stage with the risk of life-threatening tonsillar herniation. the purpose of this retrospective study was to describe our experience in the management of four cases of abscess located in the posterior cerebral fossa between january 2000 and december 2004. all patients benefited from clinical examination and radiological study (ct-scan). surgical treatment performed in a ... | 2007 | 18225734 |
| clonality of providencia stuartii isolates involved in outbreak that occurred in a burn unit. | in order to investigate an outbreak of multidrug-resistant providencia stuartii that occurred in a burn unit, we analyzed by pulsed-field gel electrophoresis (pfge) all isolates of p. stuartii collected during 4 months of 2005 from patients and from a tracheal aspirator. seventeen clinical isolates of p. stuartii, extended-spectrum beta-lactamase (esbl) producing, were collected from 17 patients. all these isolates were nosocomially acquired. three other isolates were collected from the aspirato ... | 2008 | 18241997 |
| molecular identification of dominant microflora associated with 'hawaijar' - a traditional fermented soybean (glycine max (l.)) food of manipur, india. | the dominant microorganisms in 'hawaijar', a traditional non-salted fermented soybean (glycine max (l.)) food of manipur, india, were isolated and identified by molecular techniques. bacillus spp. were the predominant microorganisms in 'hawaijar'. a total of 274 isolates were obtained from forty-one 'hawaijar' samples collected from household preparations and markets of valley districts of manipur. phenotypic grouping followed by amplified ribosomal dna restriction digestion analysis (ardra), pc ... | 2008 | 18281117 |
| the evolutionary costs of immunological maintenance and deployment. | the evolution of disease resistance and immune function may be limited if increased immunocompetence comes at the expense of other fitness-determining traits. both the maintenance of an immune system and the deployment of an immune response can be costly, and the observed costs may be evaluated as either physiological or evolutionary in origin. evolutionary costs of immunological maintenance are revealed as negative genetic correlations between immunocompetence and fitness in the absence of infe ... | 2008 | 18315877 |
| multidrug-resistant providencia stuartii expressing extended-spectrum beta-lactamase per-1, originating in kosovo. | 2008 | 18334492 | |
| genotype-by-environment interactions and adaptation to local temperature affect immunity and fecundity in drosophila melanogaster. | natural populations of most organisms harbor substantial genetic variation for resistance to infection. the continued existence of such variation is unexpected under simple evolutionary models that either posit direct and continuous natural selection on the immune system or an evolved life history "balance" between immunity and other fitness traits in a constant environment. however, both local adaptation to heterogeneous environments and genotype-by-environment interactions can maintain genetic ... | 2008 | 18369474 |
| [nationwide surveillance of parenteral antibiotics containing meropenem activities against clinically isolated strains in 2006]. | the antibacterial activity of meropenem (mepm) and other parenteral antibiotics against clinical isolates of 876 strains of gram-positive bacteria, 1764 strains of gram-negative bacteria, and 198 strains of anaerobic bacteria obtained from 30 medical institutions during 2006 was measured. the results were as follows; 1. mepm was more active than the other carbapenem antibiotics tested against gram-negative bacteria, especially against enterobacteriaceae and haemophilus influenzae. mepm was also ... | 2007 | 18447206 |
| c-3 substituted lawsonemonoximates of holmium(iii): synthesis, characterization, and antimicrobial activity. | a series of five new metal complexes of ho(iii) with c-3 substituted derivatives of lawsonemonexime (2-hydroxy-1,4-naphthalenediene-1-oxime) were synthesized. the compounds were characterized by melting point, elemental analysis, ir spectroscopy and magnetic susceptibility. the antimicrobial activity of the compounds were determined by disk diffusion method and broth micro-dilution techniques using mueller hinton medium against the following organisms: s. aureus atcc 6538p, klebsiella pneumoniae ... | 2000 | 18475938 |
| the copper-resistant bacterium acu isolated from the rhizosphere of eichhornia crassipes (mart.) increased the endurance of potamogeton crispus l. to copper toxicity. | this study aimed to develop endurance to copper stress in potamogeton crispus l. by inoculation with the anti-copper strain acu - a novel enterobacteriaceae bacterium isolated from the rhizosphere of eichhornia crassipes with high copper-removal ability. | 2008 | 18489563 |
| enterobacteriaceae and related organisms isolated from shell eggs collected during commercial processing. | in the united states, commercial shell eggs are washed and graded before retail. since passage of the egg products inspection act in 1971, processing guidelines have been set to ensure that external and internal characteristics are maintained. however, less is known about how commercial processing affects the safety of shell eggs. to identify enteric bacteria entering plants and persisting throughout processing, eggs were collected from 3 us commercial shell egg-processing plants on 3 separate v ... | 2008 | 18493013 |
| full structure of the carbohydrate chain of the lipopolysaccharide of providencia rustigianii o34. | a lipopolysaccharide isolated from an opportunistic pathogen of the enterobacteriaceae family providencia rustigianii o34 was found to be a mixture of r-, sr-, and s-forms consisting of a lipid moiety (lipid a) that bears a core oligosaccharide, a core with one o-polysaccharide repeating unit attached, and a long-chain o-polysaccharide, respectively. the corresponding carbohydrate moieties were released from the lipopolysaccharide by mild acid hydrolysis and studied by sugar and methylation anal ... | 2008 | 18512865 |
| comparative in vitro studies on the beta-lactamase-inhibiting effect of clavulanic acid and sulbactam on ampicillin-resistant enterobacteriaceae. | clavulanic acid and sulbactam alone are ineffective against pseudomonas and enterococci. staphylococci and enterobacteriaceae are inhibited by high concentrations not attainable under clinical conditions. strains of acinetobacter, especially a. lwoffii are susceptible to clavulanic acid and highly susceptible to sulbactam. the ampicillin/sulbactam combination is superior to ampicillin/clavulanic acid against citrobacter freundii, enterobacter spp. and morganella morganii. the latter combination ... | 1996 | 18611726 |
| [intestinal enterobacteria of the hibernating apis mellifera mellifera l. bees]. | dynamics of enterobacteria of normal intestinal microflora was studied in apis mellifera mellifera l. bees hibernating under snow in the western urals. the cell numbers (n) of the predominant species klebsiella oxytoca increased from 10-10(6) cfu/bee in november 2004 to 10(4)-10(7) cfu/bee in march 2005; its frequency of occurrence (p) increased from 92 to 100%. increase of providencia rettgeri (11.2004: n up to 10(6), p 25%; 03.2005: n 10(2)-10(6), p 80%) was accompanied by the substitution of ... | 2008 | 18683661 |
| effect of triclosan on the formation of crystalline biofilms by mixed communities of urinary tract pathogens on urinary catheters. | the crystalline bacterial biofilms that encrust foley catheters compromise the care of many elderly and disabled patients. the aim of this study was to examine whether the biocide triclosan can prevent encrustation by the mixed flora of uropathogens that commonly infect patients undergoing long-term catheterization. models of the catheterized bladder were inoculated with communities of organisms isolated from patients who were experiencing catheter blockage. the catheter retention balloons were ... | 2008 | 18719184 |
| structure of the o-polysaccharide of providencia alcalifaciens o8 containing (2s,4r)-2,4-dihydroxypentanoic acid, a new non-sugar component of bacterial glycans. | a glycerol teichoic acid-like o-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of providencia alcalifaciens o8 and studied by chemical methods and nmr spectroscopy, including 2d roesy, {(1)h,(13)c} hsqc, and hmqc-tocsy experiments. it was found that the compound contains a new component of bacterial lipopolysaccharides: ether-linked (2s,4r)-2,4-dihydroxypentanoic acid (dhpa), which was identified by nmr spectroscopy. the following structure of the repeating unit o ... | 2008 | 18768172 |
| prevalence and beta-lactamase producing bacterial isolates in a teaching hospital in peshawar, pakistan: a four year study. | prevalence of microorganisms was studies in clinical samples of various body fluids (n=12,259), collected from patients at post graduate medical institute hayatabad medical complex (pgmi, hmc) peshawar. only 34.90% of samples exhibited growth, 36.25% of these isolates were gram positive and 63.75% were gram negative bacteria. e. coli was the most prevalent organism (39.45%) followed by s. aureus (32.23%), proteus spp. (9.23%), pseudomonas spp. (6.54%), streptococuss spp. (3.51%), acinetobacter s ... | 2008 | 18930859 |
| studies on antimicrobial activity of perionyx excavatus. | the antimicrobial activity of vermiextract was assessed against four human pathogenic microorganisms. especially strains of proteus species are a prominent cause of urinary tract infection in children. the present investigation deals with particular urinary tract pathogenic organisms like e. coli, proteus, providencia, morganella species. screening was performed by disc diffusion method. the zone of inhibition range from 17 to 19 mm against 1.0x10(8) cells of each culture at 1 mg concentration o ... | 2007 | 19090177 |
| a blaveb-1 variant, blaveb-6, associated with repeated elements in a complex genetic structure. | bla(veb-6) was found on the proteus mirabilis chromosome in a context similar to those of bla(veb-1a) and bla(veb-1b), in a truncated gene cassette flanked by 135-bp elements and duplications of the 3'-conserved segment of class 1 integrons. a linked aaca4-aadb-dfra1-orfc cassette array includes components of tn1331, illustrating the complex mosaicism of multiresistance regions. | 2009 | 19139283 |
| screening and selection of marine isolate for l-glutaminase production and media optimization using response surface methodology. | the current work details the screening of about 400 marine isolates from various marine niches, from which one isolate was finally selected based on the productivity of glutaminase (71.23 u/l). further, biochemical identification tests and 16s rrna sequencing identified this isolate to be providencia sp. this isolate was taken up for further media optimization studies by using one-factor-at-a-time approach and subsequently by response surface methodology. a face centered central composite design ... | 2009 | 19184546 |
| structure of the o-polysaccharide from the lipopolysaccharide of providencia alcalifaciens o31 containing an ether of d-mannose with (2r,4r)-2,4-dihydroxypentanoic acid. | recently, ether-linked diastereomeric 2,4-dihydroxypentanoic acids have been reported as new components of bacterial glycans [shashkov, a. s. et al.nat. prod. commun.2008, 3, 1625-1630]. in this work, an ether of (2r,4r)-2,4-dihydroxypentanoic acid (dhpa) with d-mannose was identified in the o-polysaccharide of providencia alcalifaciens o31, and the polysaccharide structure was elucidated. studies by nmr spectroscopy confirmed the ether linkage between o-2 of dhpa and o-4 of man, and the absolut ... | 2009 | 19185853 |