Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| performance of vitek 2 for antimicrobial susceptibility testing of enterobacteriaceae with vitek 2 (2009 fda) and 2014 clsi breakpoints. | vitek 2 (biomérieux inc., durham, nc) is a widely used commercial antimicrobial susceptibility test system. we compared the mic results obtained using the vitek 2 ast-gn69 and ast-xn06 cards to those obtained by clsi broth microdilution (bmd) for 255 isolates of enterobacteriaceae, including 25 isolates of carbapenem-resistant enterobacteriaceae. in total, 25 antimicrobial agents were examined. for 10 agents, the mic data were evaluated using two sets of breakpoints: (i) the vitek 2 breakpoints, ... | 2014 | 25540403 |
| micro-organisms associated with febrile neutropenia in patients with haematological malignancies in a tertiary care hospital in eastern india. | there is paucity of information from eastern india with regard to observed dominant micro-organisms causing febrile neutropenia (fn) in patients with haematological malignancies. to identify the prevalence of pathogenic microorganisms associated with fn. a total number of 268 episodes of fn were analysed from september'2010 to october'2013. the blood samples were inoculated into brain heart infusion broth, glucose broth, hicombi dual performance media (himedia, lq-12) at 37° c for 168 h and bact ... | 2014 | 25548444 |
| micro-organisms associated with febrile neutropenia in patients with haematological malignancies in a tertiary care hospital in eastern india. | there is paucity of information from eastern india with regard to observed dominant micro-organisms causing febrile neutropenia (fn) in patients with haematological malignancies. to identify the prevalence of pathogenic microorganisms associated with fn. a total number of 268 episodes of fn were analysed from september'2010 to october'2013. the blood samples were inoculated into brain heart infusion broth, glucose broth, hicombi dual performance media (himedia, lq-12) at 37° c for 168 h and bact ... | 2014 | 25548444 |
| antimicrobial heteroresistance: an emerging field in need of clarity. | "heteroresistance" describes a phenomenon where subpopulations of seemingly isogenic bacteria exhibit a range of susceptibilities to a particular antibiotic. unfortunately, a lack of standard methods to determine heteroresistance has led to inappropriate use of this term. heteroresistance has been recognized since at least 1947 and occurs in gram-positive and gram-negative bacteria. its clinical relevance may be considerable, since more resistant subpopulations may be selected during antimicrobi ... | 2015 | 25567227 |
| in vitro spectrum of pexiganan activity when tested against pathogens from diabetic foot infections and with selected resistance mechanisms. | pexiganan, a 22-amino-acid synthetic cationic peptide, is currently in phase 3 clinical trials as a topical antimicrobial agent for the treatment of mild infections associated with diabetic foot ulcers. bacterial isolates from the 2013 sentry antimicrobial surveillance program designated as pathogens from diabetic foot infections (dfi) and gram-negative and -positive pathogens from various infection types that harbored selected resistance mechanisms/phenotypes were tested against pexiganan in re ... | 2015 | 25583717 |
| multicenter evaluation of the biofire filmarray gastrointestinal panel for etiologic diagnosis of infectious gastroenteritis. | the appropriate treatment and control of infectious gastroenteritis depend on the ability to rapidly detect the wide range of etiologic agents associated with the disease. clinical laboratories currently utilize an array of different methodologies to test for bacterial, parasitic, and viral causes of gastroenteritis, a strategy that suffers from poor sensitivity, potentially long turnaround times, and complicated ordering practices and workflows. additionally, there are limited or no testing met ... | 2015 | 25588652 |
| heterotaxy syndrome infants are at risk for early shunt failure after ladd procedure. | cardiac-specific risks and complications after a ladd procedure in patients with heterotaxy syndrome (hs) and intestinal rotational anomalies (ira) are unknown. we sought to (1) describe rates of hospital mortality and early systemic-to-pulmonary (s-p) artery shunt failure after the ladd procedure in patients with hs and (2) explore risk factors associated with early shunt failure in patients with hs with single ventricle (sv). | 2015 | 25595831 |
| genome rearrangements can make and break small rna genes. | small rnas (srnas) are short, transcribed regulatory elements that are typically encoded in the intergenic regions (igrs) of bacterial genomes. several srnas, first recognized in escherichia coli, are conserved among enteric bacteria, but because of the regulatory roles of srnas, differences in srna repertoires might be responsible for features that differentiate closely related species. we scanned the e. coli mg1655 and salmonella enterica typhimurium genomes for nonsyntenic igrs as a potential ... | 2015 | 25601101 |
| the ongoing revolution of maldi-tof mass spectrometry for microbiology reaches tropical africa. | matrix-assisted laser desorption ionization time-of-flight (maldi-tof) mass spectrometry (ms) represents a revolution in routine pathogen identification in clinical microbiology laboratories. a maldi-tof ms was introduced to tropical africa in the clinical microbiology laboratory of the hôpital principal de dakar (senegal) and used for routine pathogen identification. using ms, 2,429 bacteria and fungi isolated from patients were directly assayed, leading to the identification of 2,082 bacteria ... | 2015 | 25601995 |
| comparison of surface swab and quantitative biopsy cultures dependent on isolated microorganisms from burn wounds. | infections are one of the most important and potentially serious complications of burn wounds. quantitative biopsy cultures are useful for showing that a burn wound infection is not present, but these cultures are not useful for showing that an infection is present. swab cultures are used to diagnosis surface colonization and may not accurately reflect the organism(s) causing the wound infection. | 2013 | 25610245 |
| the identification and characterization of non-coding and coding rnas and their modified nucleosides by mass spectrometry. | the analysis of ribonucleic acids (rna) by mass spectrometry has been a valuable analytical approach for more than 25 years. in fact, mass spectrometry has become a method of choice for the analysis of modified nucleosides from rna isolated out of biological samples. this review summarizes recent progress that has been made in both nucleoside and oligonucleotide mass spectral analysis. applications of mass spectrometry in the identification, characterization and quantification of modified nucleo ... | 2014 | 25616408 |
| the identification and characterization of non-coding and coding rnas and their modified nucleosides by mass spectrometry. | the analysis of ribonucleic acids (rna) by mass spectrometry has been a valuable analytical approach for more than 25 years. in fact, mass spectrometry has become a method of choice for the analysis of modified nucleosides from rna isolated out of biological samples. this review summarizes recent progress that has been made in both nucleoside and oligonucleotide mass spectral analysis. applications of mass spectrometry in the identification, characterization and quantification of modified nucleo ... | 2014 | 25616408 |
| the bacterial curli system possesses a potent and selective inhibitor of amyloid formation. | curli are extracellular functional amyloids that are assembled by enteric bacteria during biofilm formation and host colonization. an efficient secretion system and chaperone network ensures that the major curli fiber subunit, csga, does not form intracellular amyloid aggregates. we discovered that the periplasmic protein csgc was a highly effective inhibitor of csga amyloid formation. in the absence of csgc, csga formed toxic intracellular aggregates. in vitro, csgc inhibited csga amyloid forma ... | 2015 | 25620560 |
| blocking yersiniabactin import attenuates extraintestinal pathogenic escherichia coli in cystitis and pyelonephritis and represents a novel target to prevent urinary tract infection. | the emergence and spread of extended-spectrum beta-lactamases and carbapenemases among common bacterial pathogens are threatening our ability to treat routine hospital- and community-acquired infections. with the pipeline for new antibiotics virtually empty, there is an urgent need to develop novel therapeutics. bacteria require iron to establish infection, and specialized pathogen-associated iron acquisition systems like yersiniabactin, common among pathogenic species in the family enterobacter ... | 2015 | 25624354 |
| citrobacter koseri folliculitis of the face. | we report a case of severe citrobacter koseri folliculitis of the face in a boy with acne. a 15-year-old boy affected by acne was admitted because of a rash located on the face. dermatological examination revealed two large plaques, with numerous pustules, eschars and crusts, located bilaterally and symmetrically on the cheeks. three bacteriological examinations were positive for c. koseri. the patient was successfully treated with i.m. ceftriaxone. c. koseri is a gram-negative, aerobic, mobile, ... | 2015 | 25630477 |
| the alpha-defensin test for periprosthetic joint infection responds to a wide spectrum of organisms. | the alpha-defensin test has been previously demonstrated to be highly accurate in the diagnosis of prosthetic joint infection (pji), nearly matching the musculoskeletal infection society definition for pji. however, the relationship between alpha-defensin levels and differing infecting organism has not yet been investigated. | 2015 | 25631170 |
| association of tellurite resistance with hypervirulent clonal groups of klebsiella pneumoniae. | tellurite-based selective growth media are used for several bacterial pathogens. we found that, in klebsiella pneumoniae, tellurite resistance is strongly associated with hypervirulent clonal group 23 (cg23), cg65, and cg86, providing a novel approach for screening environmental or carriage samples. the terw gene was also associated with these groups. | 2015 | 25631812 |
| lifesaving pericardiocentesis due to purulent pericarditis with growth of gram-negative rods in an immune-competent inuit male. | polymicrobial gram-negative pericarditis is a rare entity in the immune-competent patient, and purulent pericarditis due to bacteria complicated by tamponade is a life-threatening condition with high mortality rates. a prompt diagnosis and treatment is, as in this case, lifesaving and facilitated by echocardiography but is not the case in rural areas in common. change in the infectious aetiology indicates broad-spectrum antibiotics. we describe a case of purulent pericarditis causing cardiac tam ... | 2014 | 25635186 |
| propensity-matched analysis to compare the therapeutic efficacies of cefuroxime versus cefotaxime as initial antimicrobial therapy for community-onset complicated nonobstructive acute pyelonephritis due to enterobacteriaceae infection in women. | this study was performed to compare the therapeutic efficacy of cefuroxime with that of cefotaxime as initial antimicrobial therapies in women with complicated nonobstructive acute pyelonephritis (apn) caused by enterobacteriaceae infections. the clinical characteristics and outcomes of a cefuroxime-treated group (n = 156) were compared with those of a cefotaxime-treated group (n = 166). of these 322 women, 90 from each group were matched by propensity scores. the defervescence rates were not si ... | 2015 | 25645837 |
| ceftaroline activity against bacterial pathogens frequently isolated in u.s. medical centers: results from five years of the aware surveillance program. | a total of 84,704 isolates were collected from 191 medical centers in 2009 to 2013 and tested for susceptibility to ceftaroline and comparator agents by broth microdilution methods. ceftaroline inhibited all staphylococcus aureus isolates at ≤2 μg/ml and was very active against methicillin-resistant strains (mic at which 90% of the isolates tested are inhibited [mic90], 1 μg/ml; 97.6% susceptible). among streptococcus pneumoniae isolates, the highest ceftaroline mic was 0.5 μg/ml, and ceftarolin ... | 2015 | 25645844 |
| dysbiosis of the gut microbiota in disease. | there is growing evidence that dysbiosis of the gut microbiota is associated with the pathogenesis of both intestinal and extra-intestinal disorders. intestinal disorders include inflammatory bowel disease, irritable bowel syndrome (ibs), and coeliac disease, while extra-intestinal disorders include allergy, asthma, metabolic syndrome, cardiovascular disease, and obesity. | 2015 | 25651997 |
| corneal laceration caused by river crab. | a 5-year-old boy presented with right eye pain associated with tearing and photophobia of 1-day duration. he gave a history of playing with a river crab when suddenly the crab clamped his fingers. he attempted to fling the crab off, but the crab flew and hit his right eye. ocular examination revealed a right eye corneal ulcer with clumps of fibrin located beneath the corneal ulcer and 1.6 mm level of hypopyon. at presentation, the seidel test was negative, with a deep anterior chamber. culture f ... | 2015 | 25678769 |
| novel approaches to improve the intrinsic microbiological safety of powdered infant milk formula. | human milk is recognised as the best form of nutrition for infants. however; in instances where breast-feeding is not possible, unsuitable or inadequate, infant milk formulae are used as breast milk substitutes. these formulae are designed to provide infants with optimum nutrition for normal growth and development and are available in either powdered or liquid forms. powdered infant formula is widely used for convenience and economic reasons. however; current manufacturing processes are not capa ... | 2015 | 25685987 |
| rapid detection of esbl-producing enterobacteriaceae in blood cultures. | we rapidly identified extended-spectrum β-lactamase (esbl) producers prospectively among 245 gram-negative bacilli-positive cultured blood specimens using the rapid esbl nordmann/dortet/poirel test and direct bacterial identification using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. this combination identified esbl-producing enterobacteriaceae within 30 min and had high predictive values. | 2015 | 25695535 |
| positive predictive values of the international classification of diseases, 10th revision diagnoses of gram-negative septicemia/sepsis and urosepsis for presence of gram-negative bacteremia. | health care databases are a valuable resource for infectious disease epidemiology if diagnoses are accurately coded. we examined the ability of diagnostic coding to accurately identify gram-negative bacteremia. | 2015 | 25709502 |
| the complications of sinusitis in a tertiary care hospital: types, patient characteristics, and outcomes. | objective. to study the complications of sinusitis in a referral hospital and the outcome of the treatment according to the type of complication. methods. a retrospective study was performed on patients with sinusitis who were admitted to a referral hospital from 2003 to 2012. the data for the sinusitis patients who had complications were reviewed. results and discussion. eighty-five patients were included in the study, of whom 50 were male (58.8%). fourteen of the cases were less than 15 years ... | 2015 | 25722725 |
| carbapenem-nonsusceptible enterobacteriaceae in taiwan. | a total of 1135 carbapenem-resistant (nonsusceptible) enterobacteriaceae (cre) isolates were recovered between november 2010 and july 2012 (517 from 2010-2011 and 618 from 2012) from 4 hospitals in taiwan. carbapenemase-producing enterobacteriaceae (cpe) comprised 5.0% (57 isolates), including 17 kpc-2 (16 klebsiella pneumoniae and 1 escherichia coli), 1 ndm-1 (k. oxytoca), 37 imp-8 (26 enterobacter cloacae, 4 citrobacter freundii, 4 raoultella planticola, 1 k. pneumoniae, 1 e. coli and 1 k. oxy ... | 2015 | 25794144 |
| abscess formation within a cerebellar metastasis: case report and literature review. | the managements of brain abscesses and brain tumors including brain metastases differ greatly, especially in terms of surgical procedures. therefore, differentiating between the two is essential for assuring optimal treatment. however, on rare occasion, these two lesions coexist. only five cases with both a brain tumor and an abscess have thus, far been reported. we experienced a patient with a brain abscess within a cerebellar metastasis. | 2015 | 25799962 |
| identification of the serotypes of bacterial meningitis agents; implication for vaccine usage. | bacterial meningitis is one of the most serious infections and should be treated as emergency. as it has significant morbidity and mortality throughout the world, every country should have precise information regarding the etiological agents of disease and populations at risk to design public health prevention strategy. in the present study in addition of evaluation of common etiological agents (haemophilus influenzae, neisseria meningitidis, and streptococcus pneumoniae) in bacterial meningitis ... | 2014 | 25802702 |
| ceftazidime-avibactam activity tested against enterobacteriaceae isolates from u.s. hospitals (2011 to 2013) and characterization of β-lactamase-producing strains. | ceftazidime-avibactam (mic50/90, 0.12/0.25 μg/ml) inhibited 99.9% (20,698/20,709) of enterobacteriaceae isolates at ≤8 μg/ml. this compound was active against resistant subsets, including ceftazidime-nonsusceptible enterobacter cloacae (mic50/90, 0.25/0.5 μg/ml) and extended-spectrum β-lactamase (esbl) phenotype isolates. an esbl phenotype was noted among 12.4% (1,696/13,692 isolates from targeted species) of the isolates, including 776 escherichia coli (12.0% for this species; mic50/90, 0.12/0. ... | 2015 | 25845862 |
| conventional and molecular investigation of shigella isolates in relation to an outbreak in the area of isfahan, iran. | over 165 million cases of shigellosis occur in the world each year, mostly in developing countries. outbreaks of shigellosis are associated with poor sanitation, natural calamities, contaminated food and crowded living conditions. in late summer 2006, during the final stage of an outbreak of shigellosis at a vast region of isfahan province, naein & ardestan, our laboratory was assigned to investigate the outbreak in order to determine the causative agent. | 2013 | 25848502 |
| procalcitonin levels in gram-positive, gram-negative, and fungal bloodstream infections. | procalcitonin (pct) can discriminate bacterial from viral systemic infections and true bacteremia from contaminated blood cultures. the aim of this study was to evaluate pct diagnostic accuracy in discriminating gram-positive, gram-negative, and fungal bloodstream infections. a total of 1,949 samples from patients with suspected bloodstream infections were included in the study. median pct value in gram-negative (13.8 ng/ml, interquartile range (iqr) 3.4-44.1) bacteremias was significantly highe ... | 2015 | 25852221 |
| unraveling the kinetic diversity of microbial 3-dehydroquinate dehydratases of shikimate pathway. | 3-dehydroquinate dehydratase (dhqase) catalyzes the conversion of 3-dehydroquinic acid to 3-dehydroshikimic acid of the shikimate pathway. in this study, 3180 prokaryotic genomes were examined and 459 dhqase sequences were retrieved. based on sequence analysis and their original hosts, 38 dhqase genes were selected for chemical synthesis. the selected dhqases were translated into new dna sequences according to the genetic codon usage bias by both escherichia coli and corynebacterium glutamicum. ... | 2015 | 25852984 |
| impact of sepsis classification and multidrug-resistance status on outcome among patients treated with appropriate therapy. | to assess the impact of sepsis classification and multidrug-resistance status on outcome in patients receiving appropriate initial antibiotic therapy. | 2015 | 25855900 |
| brain abscess caused by citrobacter koseri infection in an adult. | citrobacter koseri is a gram-negative bacillus that causes mostly meningitis and brain abscesses in neonates and infants. however, brain abscess caused by citrobacter koseri infection in an adult is extremely rare, and only 2 cases have been described. here, we reported a 73-year-old male presenting with a 3-week headache. a history of diabetes mellitus was noted. the images revealed a brain abscess in the left frontal lobe and pus culture confirmed the growth of citrobacter koseri. the clinical ... | 2015 | 25864072 |
| nitrogen removal from micro-polluted reservoir water by indigenous aerobic denitrifiers. | treatment of micro-polluted source water is receiving increasing attention because of environmental awareness on a global level. we isolated and identified aerobic denitrifying bacteria zoogloea sp. n299, acinetobacter sp. g107, and acinetobacter sp. 81y and used these to remediate samples of their native source water. we first domesticated the isolated strains in the source water, and the 48-h nitrate removal rates of strains n299, g107, and 81y reached 33.69%, 28.28%, and 22.86%, respectively, ... | 2015 | 25867475 |
| dominance of imp-4-producing enterobacter cloacae among carbapenemase-producing enterobacteriaceae in australia. | the prevalence of carbapenemase-producing enterobacteriaceae (cpe) has been increasing worldwide. blaimp has been reported to be the predominant carbapenemase-encoding gene within enterobacteriaceae in australia. however, there are limited data currently available on cpe from queensland, australia. a total of 58 cpe isolates were isolated between july 2009 and march 2014 from queensland hospitals. the clonality of isolates was determined by diversilab repetitive sequence-based pcr. the isolates ... | 2015 | 25918153 |
| enterobacteriaceae isolates from the oral cavity of workers in a brazilian oncology hospital. | the evaluation of workers as potential reservoirs and disseminators of pathogenic bacteria has been described as a strategy for the prevention and control of healthcare-associated infections (hais). the aim of this study was to evaluate the presence of enterobacteriaceae in the oral cavity of workers at an oncology hospital in the midwest region of brazil, as well as to characterize the phenotypic profile of the isolates. saliva samples of 294 workers from the hospital's healthcare and support t ... | 2015 | 25923890 |
| efficient detection of carbapenemase activity in enterobacteriaceae by matrix-assisted laser desorption ionization-time of flight mass spectrometry in less than 30 minutes. | the recognition of carbapenemase-producing enterobacteriaceae (cpe) isolates is a major laboratory challenge, and their inappropriate or delayed detection may have negative impacts on patient management and on the implementation of infection control measures. we describe here a matrix-assisted laser desorption ionization-time of flight (maldi-tof)-based method to detect carbapenemase activity in enterobacteriaceae. after a 20-min incubation of the isolate with 0.5 mg/ml imipenem at 37°c, superna ... | 2015 | 25926485 |
| procalcitonin and pyuria-based algorithm reduces antibiotic use in urinary tract infections: a randomized controlled trial. | urinary tract infections (utis) are common drivers of antibiotic use. the minimal effective duration of antibiotic therapy for utis is unknown, but any reduction is important to diminish selection pressure for antibiotic resistance, costs, and drug-related side-effects. the aim of this study was to investigate whether an algorithm based on procalcitonin (pct) and quantitative pyuria reduces antibiotic exposure. | 2015 | 25934044 |
| nitrogen removal characteristics of a newly isolated indigenous aerobic denitrifier from oligotrophic drinking water reservoir, zoogloea sp. n299. | nitrogen is considered to be one of the most widespread pollutants leading to eutrophication of freshwater ecosystems, especially in drinking water reservoirs. in this study, an oligotrophic aerobic denitrifier was isolated from drinking water reservoir sediment. nitrogen removal performance was explored. the strain was identified by 16s rrna gene sequence analysis as zoogloea sp. n299. this species exhibits a periplasmic nitrate reductase gene (napa). its specific growth rate was 0.22 h-1. obvi ... | 2015 | 25946341 |
| clinical characteristics and risk factors of pyogenic spondylitis caused by gram-negative bacteria. | there are limited data describing the clinical characteristics of pyogenic spondylitis caused by gram-negative bacteria (gnb). the aim of this study was to investigate the predisposing factors and clinical characteristics of pyogenic spondylitis caused by gnb compared to gram-positive cocci (gpc). | 2015 | 25978839 |
| hydropneumonephroureter and emphysematous cystitis. | 2010 | 25984135 | |
| hydropneumonephroureter and emphysematous cystitis. | 2010 | 25984135 | |
| identification and clinical significance of helcococcus kunzii in human samples. | from 2008 to 2013, 39 helcococcus kunzii strains were collected from human clinical specimens (79% from foot ulcers), and 85% of the 39 patients were infected. matrix-assisted laser desorption ionization-time of flight (maldi-tof) mass spectrometry and molecular methods accurately identified all isolates. this large study of clinical observations confirms the potential pathogenic role of h. kunzii. | 2015 | 25994161 |
| evaluation of new biomérieux chromogenic cps media for detection of urinary tract pathogens. | four chromogenic media were compared for their ability to detect urinary tract pathogens in 299 urine specimens, of which 175 were found positive, allowing the growth of 279 microorganisms. after 18 to 24 h of incubation, the cps id4, cpse, cpso (biomérieux), and uriselect4 (bio-rad) media showed sensitivities of 97.1%, 99.3%, 99.6%, and 99.6%, respectively. | 2015 | 25994162 |
| a comparison of the clinical characteristics of elderly and non-elderly women with community-onset, non-obstructive acute pyelonephritis. | acute pyelonephritis (apn) is the most common cause of community-onset bacteremia in hospitalized elderly patients. the objectives of this study were to investigate the differences in the clinical and microbiological data of hospitalized elderly and non-elderly women with community-onset apn. | 2015 | 25995668 |
| microbial inhibition of oral epithelial wound recovery: potential role for quorum sensing molecules? | awareness of the impact of microbiota in both health and disease is growing. using a new in vitro oral mucosa co-culture model, we recently showed a clear inhibition of epithelial wound healing in the presence of an oral microbial community. in this paper, we have used the same model in combination with specific oral microbial species to obtain a better insight into the role of the oral microbiota in wound healing. monocultures of klebsiella oxytoca and lactobacillus salivarius significantly inh ... | 2015 | 25995981 |
| the antimicrobial activity of heterotrophic bacteria isolated from the marine sponge erylus deficiens (astrophorida, geodiidae). | interest in the study of marine sponges and their associated microbiome has increased both for ecological reasons and for their great biotechnological potential. in this work, heterotrophic bacteria associated with three specimens of the marine sponge erylus deficiens, were isolated in pure culture, phylogenetically identified and screened for antimicrobial activity. the isolation of bacteria after an enrichment treatment in heterotrophic medium revealed diversity in bacterial composition with o ... | 2015 | 25999928 |
| microbial source tracking in adjacent karst springs. | modern man-made environments, including urban, agricultural, and industrial environments, have complex ecological interactions among themselves and with the natural surroundings. microbial source tracking (mst) offers advanced tools to resolve the host source of fecal contamination beyond indicator monitoring. this study was intended to assess karst spring susceptibilities to different fecal sources using mst quantitative pcr (qpcr) assays targeting human, bovine, and swine markers. it involved ... | 2015 | 26002893 |
| effect of enteral diet enriched with eicosapentaenoic acid, gamma-linolenic acid, and antioxidants in patients with sepsis-induced acute respiratory distress syndrome. | in this study, the effects of an enteral diet enriched with eicosapentaenoic acid (epa), γ-linolenic acid (gla), and antioxidants were compared with a standard enteral diet in critically ill patients with sepsis-induced acute respiratory distress syndrome (ards). | 2015 | 26015869 |
| point-of-care multiplex pcr promises short turnaround times for microbial testing in hospital-acquired pneumonia--an observational pilot study in critical ill patients. | the early beginning of an adequate antibiotic therapy is crucial in hospital-acquired pneumonia (hap), but depends on the results of conventional microbiological diagnostics (cmd). it was the aim of this study to evaluate the performance and turnaround times of a new point-of-care multiplex polymerase chain reaction (mpcr) system for rapid identification of pathogens and antibiotic resistance markers. we assessed the applicability of the system under real-life conditions in critical ill patients ... | 2015 | 26071191 |
| bacterial epidemiology and antimicrobial resistance in the surgery wards of a large teaching hospital in southern italy. | surgical infections represent an increasingly important problem for the national health system. in this study we retrospectively evaluated the bacterial epidemiology and antimicrobial susceptibility of the microorganisms concerned as well as the utilization of antibiotics in the general and emergency surgery wards of a large teaching hospital in southern italy in the period 2011-2013. | 2015 | 26075047 |
| epidemiology and characteristics of urinary tract infections in children and adolescents. | urinary tract infections (utis) are among the most common infections in the pediatric population. over the last two decades, antibiotic resistance is increasing significantly as extended spectrum beta lactamase (esbl) producing organisms are emerging. the aim of this study is to provide a comprehensive view of the epidemiologic characteristics of utis in hospitalized children, examine the risk factors of utis caused by esbl-producing organisms, and determine the resistance patterns in the isolat ... | 2015 | 26075187 |
| mobile genetic elements related to the diffusion of plasmid-mediated ampc β-lactamases or carbapenemases from enterobacteriaceae: findings from a multicenter study in spain. | we examined the genetic context of 74 acquired ampc genes and 17 carbapenemase genes from 85 of 640 enterobacteriaceae isolates collected in 2009. using s1 pulsed-field gel electrophoresis and southern hybridization, 37 of 74 bla ampc genes were located on large plasmids of different sizes belonging to six incompatibility groups. we used sequencing and pcr mapping to investigate the regions flanking the acquired ampc genes. the bla cmy-2-like genes were associated with isecp1; the surrounding bl ... | 2015 | 26077249 |
| markers of oxidative stress and neuroprogression in depression disorder. | major depression is multifactorial disorder with high prevalence and alarming prognostic in the nearest 15 years. several mechanisms of depression are known. neurotransmitters imbalance and imbalance between neuroprogressive and neuroprotective factors are observed in major depression. depression is accompanied by inflammatory responses of the organism and consequent elevation of proinflammatory cytokines and increased lipid peroxidation are described in literature. neuropsychiatric disorders in ... | 2015 | 26078821 |
| comparison of sublingual therapeutic vaccine with antibiotics for the prophylaxis of recurrent urinary tract infections. | to compare the clinical impact of a prophylactic treatment with sublingual immunostimulation in the prevention of recurrent urinary tract infections (rutis) with the use of antibiotics. | 2015 | 26090341 |
| fusobacterium nucleatum: a rare cause of pyogenic liver abscess. | while pyogenic liver abscesses are uncommon, they are associated with significant mortality and morbidity. most pyogenic liver abscesses are polymicrobial and are caused by enteric bacteria and anaerobes. rarely, mono-microbial infections may occur, typically in immunocompromised individuals. we report the unusual case of a 69 year-old immunocompetent female who developed a pyogenic liver abscess due to fusobacterium nucleatum infection, likely from a dental source. poor oropharyngeal hygiene se ... | 2015 | 26101735 |
| direct identification of urinary tract pathogens from urine samples using the vitek ms system based on matrix-assisted laser desorption ionization-time of flight mass spectrometry. | we evaluated the coincidence rate between vitek ms system (biomérieux, france) and vitek 2 in identifying uropathogens directly from urine specimens. | 2015 | 26131413 |
| occurrence and characterization of cronobacter spp. in dehydrated rice powder from chinese supermarket. | cronobacter spp. are emerging food-borne pathogens and have been identified as causative agents of meningitis and necrotizing enterocolitis in infants. dehydrated rice is popular with a wide range of people and it is frequently used as a substitute for infant milk powder to baby older than four months. the occurrence of cronobacter spp. was investigated in 1,012 samples of dehydrated rice powder collected from 14 manufacturers in china during 2010 to 2012. the isolates were identified using fusa ... | 2015 | 26132635 |
| detection of an inca/c plasmid encoding vim-4 and cmy-4 β-lactamases in klebsiella oxytoca and citrobacter koseri from an inpatient in a cardiac rehabilitation unit. | a 62-year-old patient was transferred to the cardiac rehabilitation unit of the i.r.c.c.s. fondazione s. maugeri after undergoing a heart transplantation at the acute care hospital i.r.c.c.s. s. matteo of pavia. on 1 august 2013 and during hospitalization in the rehabilitation unit, klebsiella oxytoca and citrobacter koseri clinical isolates were simultaneously recovered from the patient's preputial swab. both the k. oxytoca and c. koseri strains were carbapenem- resistant by microscan system (b ... | 2015 | 26147151 |
| multiwavelength resonance raman characterization of the effect of growth phase and culture medium on bacteria. | we examine the use of multiwavelength ultraviolet (uv) resonance-raman signatures to identify the effects of growth phase and growth medium on gram-positive and gram-negative bacteria. escherichia coli (e. coli), citrobacter koseri (c. koseri), citrobacter braakii (c. braakii), and bacillus cereus (b. cereus) were grown to logarithmic and stationary phases in nutrient broth and brain heart infusion broth. resonance raman spectra of bacteria were obtained at multiple wavelengths between 220 and 2 ... | 2015 | 26163518 |
| antimicrobial tolerance in biofilms. | the tolerance of microorganisms in biofilms to antimicrobial agents is examined through a meta-analysis of literature data. a numerical tolerance factor comparing the rates of killing in the planktonic and biofilm states is defined to provide a quantitative basis for the analysis. tolerance factors for biocides and antibiotics range over three orders of magnitude. this variation is not explained by taking into account the molecular weight of the agent, the chemistry of the agent, the substratum ... | 2015 | 26185072 |
| real-time microbiology laboratory surveillance system to detect abnormal events and emerging infections, marseille, france. | infectious diseases are a major threat to humanity, and accurate surveillance is essential. we describe how to implement a laboratory data-based surveillance system in a clinical microbiology laboratory. two historical microsoft excel databases were implemented. the data were then sorted and used to execute the following 2 surveillance systems in excel: the bacterial real-time laboratory-based surveillance system (balyses) for monitoring the number of patients infected with bacterial species iso ... | 2015 | 26196165 |
| human microbiota. small molecules from the human microbiota. | developments in the use of genomics to guide natural product discovery and a recent emphasis on understanding the molecular mechanisms of microbiota-host interactions have converged on the discovery of small molecules from the human microbiome. here, we review what is known about small molecules produced by the human microbiota. numerous molecules representing each of the major metabolite classes have been found that have a variety of biological activities, including immune modulation and antibi ... | 2015 | 26206939 |
| low-level antimicrobial resistance of enterobacteriaceae isolated from the nares of pig-exposed persons. | extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae (esbl-e) have recently emerged in livestock and humans. therefore, this study assessed the carriage of enterobacteriaceae in the anterior nares and associated antimicrobial resistance in pig-exposed persons. nasal swabs were enriched in non-selective broth and then plated on macconkey and esbl-selective agars. species was confirmed by matrix-assisted laser-desorption ionization-time-of-flight mass spectrometry (maldi-tof ms). anti ... | 2016 | 26219671 |
| evaluation of two phenotypic screening tests for carbapenemase-producing enterobacteriaceae. | we evaluated the performance of two rapid tests for detection of carbapenemase-producing enterobacteriaceae (cpe) strains. the sensitivities and the specificities were 97.6% and 94.4% for the rapid carb screen and 98.8% and 93.1% for the kpc/mbl & oxa-48 confirm tests, providing the usefulness of these tools for screening cpe in microbiology wards. | 2015 | 26224841 |
| presence of antibodies to salmonella in tuatara (sphenodon punctatus) sera. | colonisation of a host by pathogenic microorganisms is a near constant threat to the health of all vertebrates and most species have evolved an efficient adaptive immune response which produces antibodies following exposure to a specific antigen. the strength of this response can be influenced by many factors including sex and season. tuatara are exposed to salmonella through contact with infected skinks and soil; however, no gastrointestinal colonisation of tuatara with salmonella has been foun ... | 2015 | 26264523 |
| ventilator-associated pneumonia caused by commensal oropharyngeal flora; [corrected] a retrospective analysis of a prospectively collected database. | the significance of commensal oropharyngeal flora (cof) as a potential cause of ventilator-associated pneumonia (vap) is scarcely investigated and consequently unknown. therefore, the aim of this study was to explore whether cof may cause vap. | 2015 | 26264828 |
| clinical performance of a matrix-assisted laser desorption ionization-time of flight mass spectrometry method for detection of certain blakpc-containing plasmids. | rapid detection of blakpc-containing organisms can significantly impact infection control and clinical practices, as well as therapeutic choices. current molecular and phenotypic methods to detect these organisms, however, require additional testing beyond routine organism identification. in this study, we evaluated the clinical performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) to detect pkpqil_p019 (p019)-an ∼11,109-da protein associated ... | 2015 | 26338858 |
| clinical performance of a matrix-assisted laser desorption ionization-time of flight mass spectrometry method for detection of certain blakpc-containing plasmids. | rapid detection of blakpc-containing organisms can significantly impact infection control and clinical practices, as well as therapeutic choices. current molecular and phenotypic methods to detect these organisms, however, require additional testing beyond routine organism identification. in this study, we evaluated the clinical performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) to detect pkpqil_p019 (p019)-an ∼11,109-da protein associated ... | 2015 | 26338858 |
| clinical performance of check-direct cpe, a multiplex pcr for direct detection of bla(kpc), bla(ndm) and/or bla(vim), and bla(oxa)-48 from perirectal swabs. | we evaluated the clinical performance of check-direct cpe for carbapenemase detection directly from 301 perirectal swabs (258 patients) in a nonoutbreak setting. culture of a pcr-confirmed, carbapenemase-containing organism, or history of colonization with such organism within the previous 2 weeks, was used as the reference standard. check-direct cpe demonstrated a sensitivity value, specificity value, positive predictive value (ppv), and negative predictive value (npv) of 100% (all bla(kpc)), 8 ... | 2015 | 26338860 |
| a comparative analysis of odontogenic maxillofacial infections in diabetic and nondiabetic patients: an institutional study. | the increased prevalence of antibiotic resistance is an outcome of evolution. most patients presenting with odontogenic space infections also have associated systemic co-morbidities such as diabetes mellitus resulting in impaired host defense. the present study aims to compare the odontogenic spaces involved, antibiotic susceptibility of microorganisms, length of hospital stay, and the infl uence of systemic comorbidities on treatment outcome in diabetic patients. | 2015 | 26339575 |
| adhesins involved in attachment to abiotic surfaces by gram-negative bacteria. | during the first step of biofilm formation, initial attachment is dictated by physicochemical and electrostatic interactions between the surface and the bacterial envelope. depending on the nature of these interactions, attachment can be transient or permanent. to achieve irreversible attachment, bacterial cells have developed a series of surface adhesins promoting specific or nonspecific adhesion under various environmental conditions. this article reviews the recent advances in our understandi ... | 2015 | 26350310 |
| characterization of the bacterioferritin/bacterioferritin associated ferredoxin protein-protein interaction in solution and determination of binding energy hot spots. | mobilization of iron stored in the interior cavity of bfrb requires electron transfer from the [2fe−2s] cluster in bfd to the core iron in bfrb. a crystal structure of the pseudomonas aeruginosa bfrb:bfd complex revealed that bfrb can bind up to 12 bfd molecules at 12 structurally identical binding sites, placing the [2fe−2s] cluster of each bfd immediately above a heme group in bfrb [yao, h., et al. (2012) j. am. chem. soc., 134, 13470−13481]. we report here study aimed at characterizing the st ... | 2015 | 26368531 |
| determinants of bacteriological outcomes in exacerbations of chronic obstructive pulmonary disease. | changes in sputum microbiology following antibiotic treatment of acute exacerbations of chronic obstructive pulmonary disease (aecopd), including patterns of bacteriological relapse and superinfection are not well understood. sputum microbiology at exacerbation is not routinely performed, but pathogen presence and species are determinants of outcomes. therefore, we determined whether baseline clinical factors could predict the presence of bacterial pathogens at exacerbation. bacterial eradicatio ... | 2015 | 26370552 |
| determinants of bacteriological outcomes in exacerbations of chronic obstructive pulmonary disease. | changes in sputum microbiology following antibiotic treatment of acute exacerbations of chronic obstructive pulmonary disease (aecopd), including patterns of bacteriological relapse and superinfection are not well understood. sputum microbiology at exacerbation is not routinely performed, but pathogen presence and species are determinants of outcomes. therefore, we determined whether baseline clinical factors could predict the presence of bacterial pathogens at exacerbation. bacterial eradicatio ... | 2015 | 26370552 |
| early detection of extended-spectrum β-lactamase from blood culture positive for an enterobacteriaceae using βlacta test. | bacterial pellets from enterobacteriaceae positive blood cultures prepared using ammonium chloride were tested for rapid detection of β-lactamase using the commercial βlacta test and read after 30 minutes. during 7 months, 137 bacterial pellets were tested prospectively. βlacta test exhibited a sensitivity of 75% and a specificity of 100% for the detection of third-generation cephalosporin resistance. false negative tests were mainly observed with hyperproduced chromosomal or plasmid-borne ampc. | 2015 | 26380714 |
| in vitro activities of ceftazidime-avibactam, aztreonam-avibactam, and a panel of older and contemporary antimicrobial agents against carbapenemase-producing gram-negative bacilli. | among 177 carbapenemase-producing gram-negative bacilli (108 kpc, 32 ndm, 11 imp, 8 oxa-48, 4 oxa-181, 2 oxa-232, 5 imi, 4 vim, and 3 sme producers), aztreonam-avibactam was active against all isolates except two ndm producers with elevated mics of 8/4 and 16/4 mg/liter; ceftazidime-avibactam was active against all kpc-, imi-, sme-, and most oxa-48 group-producing isolates (93%) but not metallo-β-lactamase producers. among older and contemporary antimicrobials, the most active were colistin, tig ... | 2015 | 26392487 |
| diverse mechanisms of post-transcriptional repression by the small rna regulator of glucose-phosphate stress. | the escherichia coli small rna sgrs controls a metabolic stress response that occurs upon accumulation of certain glycolytic intermediates. sgrs base pairs with and represses translation of ptsg and manxyz mrnas, which encode sugar transporters, and activates translation of yigl mrna, encoding a sugar phosphatase. this study defines four new genes as direct targets of e. coli sgrs. these new targets, asd, adiy, fole and purr, encode transcription factors or enzymes of diverse metabolic pathways, ... | 2015 | 26411266 |
| diverse mechanisms of post-transcriptional repression by the small rna regulator of glucose-phosphate stress. | the escherichia coli small rna sgrs controls a metabolic stress response that occurs upon accumulation of certain glycolytic intermediates. sgrs base pairs with and represses translation of ptsg and manxyz mrnas, which encode sugar transporters, and activates translation of yigl mrna, encoding a sugar phosphatase. this study defines four new genes as direct targets of e. coli sgrs. these new targets, asd, adiy, fole and purr, encode transcription factors or enzymes of diverse metabolic pathways, ... | 2015 | 26411266 |
| evaluation of the rapidec carba np test for detection of carbapenemases in enterobacteriaceae. | this study evaluated the performance of the rapidec carba np test, which was introduced recently into the market for the detection of carbapenemase production in a broad spectrum of β-lactamase-producing enterobacteriaceae clinical isolates. in total, 252 clinical enterobacteriaceae isolates that had been genetically characterized with respect to carbapenemase, extended-spectrum β-lactamase (esbl), and ampc genes were analyzed; 51/252 isolates (20.2%) were genetically confirmed to be carbapenema ... | 2015 | 26424840 |
| incidence, etiology and risk factors associated with neonatal healthcare-associated conjunctivitis: a prospective study from a tertiary care hospital in india. | healthcare-associated conjunctivitis (hac) can lead to serious sequelae including blindness. we conducted a one-year prospective study to determine the epidemiology of neonatal hac at a tertiary-care hospital in india. | 2015 | 26428196 |
| incidence, etiology and risk factors associated with neonatal healthcare-associated conjunctivitis: a prospective study from a tertiary care hospital in india. | healthcare-associated conjunctivitis (hac) can lead to serious sequelae including blindness. we conducted a one-year prospective study to determine the epidemiology of neonatal hac at a tertiary-care hospital in india. | 2015 | 26428196 |
| adverse events associated with fosfomycin use: review of the literature and analyses of the fda adverse event reporting system database. | the growing problem of antibacterial resistance resulted in an increased interest in fosfomycin, especially its parenteral formulation. we reviewed fosfomycin safety profile using the food and drug administration adverse event (ae) reporting system (faers) and published literature. | 2015 | 26437630 |
| the pla gene, encoding plasminogen activator, is not specific to yersinia pestis. | here we present evidence to show that the pla gene, previously thought to be specific to yersinia pestis, occurs in some strains of citrobacter koseri and escherichia coli. this means that detection of this gene on its own can no longer be taken as evidence of detection of y. pestis. | 2015 | 26438258 |
| unexploited opportunities for phage therapy. | 2015 | 26441644 | |
| urinary tract infections in women with stress urinary incontinence treated with transobturator suburethral tape and benefit gained from the sublingual polibacterial vaccine. | stress urinary incontinence (sui) and recurrent urinary tract infections (rutis) are highly prevalent diseases. our purpose was to investigate the relationship between rutis and surgical correction of sui with transobturator suburethral tape (tot) and to describe the benefit gained from a sublingual polibacterial preparation on rutis developed after tot. | 2015 | 26445597 |
| capsular profiling of the cronobacter genus and the association of specific cronobacter sakazakii and c. malonaticus capsule types with neonatal meningitis and necrotizing enterocolitis. | cronobacter sakazakii and c. malonaticus can cause serious diseases especially in infants where they are associated with rare but fatal neonatal infections such as meningitis and necrotising enterocolitis. | 2015 | 26449318 |
| direct capture technologies for genomics-guided discovery of natural products. | microbes are important producers of natural products, which have played key roles in understanding biology and treating disease. however, the full potential of microbes to produce natural products has yet to be realized; the overwhelming majority of natural product gene clusters encoded in microbial genomes remain "cryptic", and have not been expressed or characterized. in contrast to the fast-growing number of genomic sequences and bioinformatic tools, methods to connect these genes to natural ... | 2016 | 26456469 |
| linking biosynthetic gene clusters to their metabolites via pathway- targeted molecular networking. | the connection of microbial biosynthetic gene clusters to the small molecule metabolites they encode is central to the discovery and characterization of new metabolic pathways with ecological and pharmacological potential. with increasing microbial genome sequence information being deposited into publicly available databases, it is clear that microbes have the coding capacity for many more biologically active small molecules than previously realized. of increasing interest are the small molecule ... | 2016 | 26456470 |
| three-step biocatalytic reaction using whole cells for efficient production of tyramine from keratin acid hydrolysis wastewater. | tyramine has been paid more attention in recent years as a significant metabolite of tyrosine and catecholamine drug and an intermediate of medicinal material and some drugs. in this study, an effective, green, and three-step biocatalytic synthesis method for production of tyramine starting from serine in keratin acid hydrolysis wastewater was developed and investigated. serine deaminase from escherichia coli was first combined with tyrosine phenol-lyase from citrobacter koseri, to convert l-ser ... | 2016 | 26476652 |
| antimicrobial susceptibilities of aerobic and facultative gram-negative bacilli from intra-abdominal infections in patients from seven regions in china in 2012 and 2013. | to evaluate the antimicrobial susceptibility of gram-negative bacilli that caused hospital-acquired and community-acquired intra-abdominal infections (iais) in china between 2012 and 2013, we determined the susceptibilities to 12 antimicrobials and the extended-spectrum β-lactamase (esbl) statuses of 3,540 iai isolates from seven geographic areas in china in a central laboratory using clsi broth microdilution and interpretive standards. most infections were caused by escherichia coli (46.3%) and ... | 2015 | 26482308 |
| antimicrobial susceptibilities of aerobic and facultative gram-negative bacilli from intra-abdominal infections in patients from seven regions in china in 2012 and 2013. | to evaluate the antimicrobial susceptibility of gram-negative bacilli that caused hospital-acquired and community-acquired intra-abdominal infections (iais) in china between 2012 and 2013, we determined the susceptibilities to 12 antimicrobials and the extended-spectrum β-lactamase (esbl) statuses of 3,540 iai isolates from seven geographic areas in china in a central laboratory using clsi broth microdilution and interpretive standards. most infections were caused by escherichia coli (46.3%) and ... | 2015 | 26482308 |
| detection of carbapenemases in clinical enterobacteriaceae isolates using the vitek ast-n202 card. | the rapid and accurate detection of carbapenemase-producing enterobacteriaceae (cpe) in clinical microbiology laboratories is essential for the treatment and control of infections caused by these microorganisms. this study was performed to evaluate the ability of the vitek ast-n202 card to detect cpe isolates. | 2015 | 26483990 |
| blandm-1 carriage on incr plasmid in enterobacteriaceae strains. | four ndm-1-producing enterobacteriaceae strains (three klebsiella pneumoniae and one citrobacter koseri) were isolated between 2009 and 2011 through a nationwide surveillance for carbapenem-resistant enterobacteriaceae in croatia to study the molecular genetic background of blandm and the responsible plasmid types. phenotypically, the clinical strains proved to be multidrug resistant. all strains remained susceptible to tigecycline and colistin. the clinical strains harbored variable antibiotic ... | 2016 | 26484384 |
| endometriosis in a kidney with focal xanthogranulomatous pyelonephritis and a perinephric abscess. | the presence of endometriosis in the kidney is extremely rare. we report a case of endometriosis in renal parenchyma incidentally found in a malfunctioning kidney removed because of xanthogranulomatous pyelonephritis. | 2015 | 26490542 |
| early divergent strains of yersinia pestis in eurasia 5,000 years ago. | the bacteria yersinia pestis is the etiological agent of plague and has caused human pandemics with millions of deaths in historic times. how and when it originated remains contentious. here, we report the oldest direct evidence of yersinia pestis identified by ancient dna in human teeth from asia and europe dating from 2,800 to 5,000 years ago. by sequencing the genomes, we find that these ancient plague strains are basal to all known yersinia pestis. we find the origins of the yersinia pestis ... | 2015 | 26496604 |
| the molecular characterisation of escherichia coli k1 isolated from neonatal nasogastric feeding tubes. | the most common cause of gram-negative bacterial neonatal meningitis is e. coli k1. it has a mortality rate of 10-15 %, and neurological sequelae in 30-50 % of cases. infections can be attributable to nosocomial sources, however the pre-colonisation of enteral feeding tubes has not been considered as a specific risk factor. | 2015 | 26497222 |
| rapid screening for carbapenem resistant organisms: current results and future approaches. | carbapenem producing enterobacteriaceae (cpe) is a major public health threat. a total of 120 carbapenem resistant e.coli (n=32) and k.pneumoniae (n=88) from blood stream infections were screened for the presence of carbapenem resistant genes kpc, ndm, imp, vim, and oxa-48 like using both conventional multiplex pcr and xpert(®) carba-r test. additionally 26 faeces samples were directly screened with xpert(®) carba-r test. of the tested isolates, 40% (n=48) of ndm and 39.2% (n=47/) of oxa-48-like ... | 2015 | 26500909 |