Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| recombinant attenuated listeria monocytogenes vaccine expressing francisella tularensis iglc induces protection in mice against aerosolized type a f. tularensis. | fransicella tularensis, the causative agent of tularemia, is in the top category (category a) of potential agents of bioterrorism. to develop a safer vaccine against aerosolized f. tularensis, we have employed an attenuated listeria monocytogenes, which shares with f. tularensis an intracellular and extraphagosomal lifestyle, as a delivery vehicle for f. tularensis antigens. we constructed recombinant l. monocytogenes (rlm) vaccines stably expressing seven f. tularensis proteins including iglc ( ... | 2009 | 19126421 |
| [listeria monocytogenes induced-chronic arthritis in an immunoincompetent child]. | 2008 | 19128757 | |
| the listeria monocytogenes sortase-b recognizes varied amino acids at position 2 of the sorting motif. | sortases are bacterial enzymes that anchor surface proteins covalently to the peptidoglycan upon cleavage of a motif located at their c-terminal end. motifs recognized by sortases of the class-b (srtb) are defined by the consensus sequence np(q/k)(t/s)(n/g/s)(d/a). evidence supporting this consensus is limited to isdc of staphylococcus aureus and bacillus anthracis, cleaved at motifs npqtn and npktg, respectively. in listeria monocytogenes, strb has two substrates, lmo2185 and lmo2186, containin ... | 2009 | 19129190 |
| the cellular niche of listeria monocytogenes infection changes rapidly in the spleen. | the spleen is an important organ for the host response to systemic bacterial infections. many cell types and cell surface receptors have been shown to play role in the capture and control of bacteria, yet these are often studied individually and a coherent picture has yet to emerge of how various phagocytes collaborate to control bacterial infection. we analyzed the cellular distribution of listeria monocytogenes (lm) in situ during the early phase of infection. using an immunohistochemistry app ... | 2009 | 19130474 |
| foci of listeria monocytogenes persist in the bone marrow. | murine listeriosis is one of the most comprehensive and well-studied models of infection, and listeria monocytogenes has provided seminal information regarding bacterial pathogenesis. however, many aspects of the mouse model remain poorly understood, including carrier states and chronic colonization which represent important features of the spectrum of host-pathogen interaction. bone marrow has recently been shown to harbor l. monocytogenes, which spreads from this location to the central nervou ... | 2009 | 19132117 |
| therapeutic index of gramicidin s is strongly modulated by d-phenylalanine analogues at the beta-turn. | analogues of the cationic antimicrobial peptide gramicidin s (gs), cyclo(val-orn-leu-d-phe-pro)2, with d-phe residues replaced by different (restricted mobility, mostly) surrogates have been synthesized and used in sar studies against several pathogenic bacteria. while all d-phe substitutions are shown by nmr to preserve the overall beta-sheet conformation, they entail subtle structural alterations that lead to significant modifications in biological activity. in particular, the analogue incorpo ... | 2009 | 19132829 |
| faecal shedding and strain diversity of listeria monocytogenes in healthy ruminants and swine in northern spain. | listeria monocytogenes is among the most important foodborne bacterial pathogens due to the high mortality rate and severity of the infection. l. monocytogenes is a ubiquitous organism occasionally present in the intestinal tract of various animal species and faecal shedding by asymptomatically infected livestock poses a risk for contamination of farm environments and raw food at the pre-harvest stages. the aim of this study was to determine the prevalence and strain diversity of l. monocytogene ... | 2009 | 19133125 |
| cd40-cd40l cross-talk integrates strong antigenic signals and microbial stimuli to induce development of il-17-producing cd4+ t cells. | il-17-producing cd4(+) t cells have been recognized as key players in organ-related autoimmune disease; however, the parameters that govern their development are yet to be elucidated fully. by using both in vivo and in vitro systems, we have investigated the role of antigen dose, pathogen-associated molecular patterns, and cd40-cd40 ligand (cd40l) cross-talk in th17 differentiation. we found that the strength of antigenic stimulation critically influenced the extent of th17 differentiation, beca ... | 2009 | 19136631 |
| hierarchical generalized linear models for multiple quantitative trait locus mapping. | we develop hierarchical generalized linear models and computationally efficient algorithms for genomewide analysis of quantitative trait loci (qtl) for various types of phenotypes in experimental crosses. the proposed models can fit a large number of effects, including covariates, main effects of numerous loci, and gene-gene (epistasis) and gene-environment (g x e) interactions. the key to the approach is the use of continuous prior distribution on coefficients that favors sparseness in the fitt ... | 2009 | 19139143 |
| multiplex-pcr serotyping of listeria monocytogenes isolated from human clinical specimens. | the genus listeria is composed of six species of which listeria monocytogenes is considered the single pathogenic species that causes listeriosis in humans. of the 13 serovars of l. monocytogenes, 1/2a, 1/2b and 4b are responsible for the majority of clinical cases. the aim of this work was to detect l. monocytogenes in the cerebrospinal fluid sample of premature newborns and to characterize this sample using biotyping, serotyping and molecular typing. the results indicated the presence of l. mo ... | 2008 | 19148426 |
| [rapid detection of vibrio parahaemolyticus by taqman-based real-time pcr assay targeting the toxr gene]. | we designed a pair of specific primers and a taqman fluorescent probe targeting the toxr gene of vibrio parahaemolyticus (vp). after optimizing the conditions, the specialty, sensitivity and reproducibility of the detection method were evaluated. results: (1) the developed real-time pcr assay protocol detected only vp and was not affected by other normal food pathogens such as staphylococcus aureus, salmonela, listeria monocytogenes. (2) the limit of detection was 25 copies of toxr gene in the d ... | 2008 | 19149202 |
| role of cold shock proteins in growth of listeria monocytogenes under cold and osmotic stress conditions. | the gram-positive bacterium listeria monocytogenes is a food-borne pathogen of both public health and food safety significance. it possesses three small, highly homologous protein members of the cold shock protein (csp) family. we used gene expression analysis and a set of mutants with single, double, and triple deletions of the csp genes to evaluate the roles of cspa, cspb, and cspd in the cold and osmotic (nacl) stress adaptation responses of l. monocytogenes. all three csps are dispensable fo ... | 2009 | 19151183 |
| agrd-dependent quorum sensing affects biofilm formation, invasion, virulence and global gene expression profiles in listeria monocytogenes. | the listeria monocytogenes agr peptide-sensing system has been analysed by creating a deletion mutant in agrd, the structural gene for the putative quorum-sensing peptide. the deltaagrd mutant displayed significantly reduced biofilm formation, a defect which could be restored by genetic or physical complementation. a reduced invasion of caco-2 intestinal epithelial cells was observed for the deltaagrd mutant while phagocytosis by thp-1 macrophages was unaffected. additionally, the level of inter ... | 2009 | 19154329 |
| modeling the survival of escherichia coli o157:h7, listeria monocytogenes, and salmonella typhimurium during fermentation, drying, and storage of soudjouk-style fermented sausage. | this study quantified and modeled the survival of escherichia coli o157:h7, listeria monocytogenes and salmonella typhimurium in soudjouk-style fermented sausage during fermentation, drying, and storage. batter prepared from ground beef (20% fat), seasonings, starter culture, and dextrose was separately inoculated with a multi-strain mixture of each pathogen to an initial inoculum of ca. 6.5 log(10) cfu/g in the batter. the sausages were subsequently fermented at 24 degrees c with a relative hum ... | 2009 | 19157610 |
| different t cell receptor signals determine cd8+ memory versus effector development. | following infection, naïve cd8+ t cells bearing pathogen-specific t cell receptors (tcrs) differentiate into a mixed population of short-lived effector and long-lived memory t cells to mediate an adaptive immune response. how the tcr regulates memory t cell development has remained elusive. using a mutant tcr transgenic model, we found that point mutations in the tcr beta transmembrane domain (betatmd) impair the development and function of cd8+ memory t cells without affecting primary effector ... | 2009 | 19164748 |
| the effects of stress on respiratory disease and transient colonization of turkeys with listeria monocytogenes scott a. | listeria monocytogenes contaminates poultry processing plants due to its ubiquitous nature and high resistance to disinfection. the sources of the persistent, biofilm-forming strains that colonize processing plants are unknown. the purpose of this study is to determine if intrinsic colonization of turkeys is a possible source of contamination. male poults were subjected to cold stress from 4-12 days of age; poults were unchallenged or were exposed to an aerosol and oral challenge of either an av ... | 2008 | 19166048 |
| changes in barotolerance, thermotolerance, and cellular morphology throughout the life cycle of listeria monocytogenes. | changes in barotolerance, thermotolerance, and cellular morphology throughout the life cycle of listeria monocytogenes were investigated. for part 1 of this analysis, l. monocytogenes atcc 19115 was grown to log, stationary, death, and long-term-survival phases at 35 degrees c in tryptic soy broth with yeast extract (tsbye). cells were diluted in whole milk that had been subjected to ultrahigh temperatures (uht whole milk) and then high-pressure processed (hpp) at 400 mpa for 180 s or thermally ... | 2009 | 19168646 |
| spontaneous fermentation of traditional sago starch in papua new guinea. | sago starch is an important dietary carbohydrate in lowland papua new guinea (png). an investigation was conducted to determine whether microbes play a role in its preservation using traditional methods. in 12 stored sago samples collected from png villages, lactic acid bacteria (lab) were present (> or = 3.6 x 10(4)cfu/g) and ph ranged from 6.8 to 4.2. acetic and propionic acids were detected in all samples, while butyric, lactic and valeric acids were present in six or more. in freshly prepare ... | 2009 | 19171254 |
| effects of gamma-irradiation on listeria monocytogenes population, colour, texture and sensory properties of feta cheese during cold storage. | feta, a white brine cheese, was produced and contaminated with listeria monocytogenes. contamination occurred either at the beginning (pre-process contamination) or at the end of feta manufacturing (post-process contamination). in the first case the milk was contaminated with 10(3) cfu/ml, and 2 months later, in the final product, the l. monocytogenes population was approximately 10(5) cfu/g. in the second case, the brine (nacl, 7% w/v), in which the feta was packaged, was contaminated with 10(3 ... | 2009 | 19171257 |
| contributions to selected phenotypic characteristics of large species- and lineage-specific genomic regions in listeria monocytogenes. | we hypothesized that genomic regions specific to listeria monocytogenes or selected l. monocytogenes strains may contribute to virulence and phenotypic differences among the strains. a whole genome alignment of two completed l. monocytogenes genomes and the one completed listeria innocua genome initially identified 28 genomic regions of difference (rd) > 4 kb that were found in one or both l. monocytogenes genomes, but absent from the non-pathogenic l. innocua. in silico analyses using an additi ... | 2009 | 19171265 |
| a comparison between e-beam irradiation and high pressure treatment for cold-smoked salmon sanitation: microbiological aspects. | the effectiveness of electron beam irradiation and high pressure treatment for the sanitation of cold-smoked salmon from two points of view, microbial safety and shelf-life extension, was compared. from the response of l. monocytogenes inia h66a to irradiation, a d value of 0.51 kgy was calculated. for samples stored at 5 degrees c, 1.5 kgy would be sufficient to attain a food safety objective (fso) of 2 log(10)cfu/g l. monocytogenes for a 35-day shelf-life, whereas 3 kgy would be needed in the ... | 2009 | 19171266 |
| prevalence and risk factors for bacterial food-borne zoonotic hazards in slaughter pigs: a review. | the hygiene package and regulation ec-2160/2003 require information flow from farm to slaughterhouse to enhance european consumers protection in a 'farm to fork' approach. this obligation especially concerns food-borne zoonotic hazards transmitted to humans through pork consumption, such as thermophilic campylobacter spp., listeria monocytogenes, salmonella enterica and yersinia enterocolitica. prevalence estimates of these four hazards are affected by the sampling strategy and diagnostic proced ... | 2009 | 19175574 |
| multiple mechanisms contribute to the robust rapid gamma interferon response by cd8+ t cells during listeria monocytogenes infection. | a subset of cd8+ t cells can rapidly secrete gamma interferon (ifn-gamma) in an antigen-independent and interleukin-12 (il-12)- and il-18-dependent manner within 16 h of infection with the intracellular bacterial pathogen listeria monocytogenes. this rapid ifn-gamma response is robust enough to be detected directly ex vivo and is not observed following infection with intracellular bacterial pathogens that remain sequestered within host cell vacuoles. we demonstrate here that three distinct pathw ... | 2009 | 19179413 |
| a novel il-17-dependent mechanism of cross protection: respiratory infection with mycoplasma protects against a secondary listeria infection. | immune responses to pathogens occur within the context of current and previous infections. cross protection refers to the phenomena where infection with a particular pathogen provides enhanced resistance to a subsequent unrelated pathogen in an antigen-independent manner. proposed mechanisms of antigen-independent cross protection have involved the secretion of ifn-gamma, which activates macrophages, thus providing enhanced innate immunity against the secondary viral or bacterial pathogen. here ... | 2009 | 19180464 |
| both thiamine uptake and biosynthesis of thiamine precursors are required for intracellular replication of listeria monocytogenes. | thiamine pyrophosphate is an essential cofactor involved in central metabolism and amino acid biosynthesis and is derived from thiamine (vitamin b(1)). the extent to which this metabolite is available to bacterial pathogens replicating within host cells is still little understood. growth studies using modified minimal welshimer's broth (mmwb) supplemented with thiamine or the thiamine precursor hydroxymethylpyrimidine (hmp) showed that listeria monocytogenes, in agreement with bioinformatic pred ... | 2009 | 19181806 |
| generation of branched-chain fatty acids through lipoate-dependent metabolism facilitates intracellular growth of listeria monocytogenes. | the gram-positive bacterial pathogen listeria monocytogenes has evolved mechanisms to rapidly replicate in the host cytosol, implying efficient utilization of host-derived nutrients. however, the contribution of host nutrient scavenging versus that of bacterial biosynthesis toward rapid intracellular growth remains unclear. nutrients that contribute to growth of l. monocytogenes include branched-chain fatty acids (bcfas), amino acids, and other metabolic intermediates generated from acyl-coenzym ... | 2009 | 19181817 |
| stress- and growth rate-related differences between plate count and real-time pcr data during growth of listeria monocytogenes. | to assess the overestimation of bacterial cell counts in real-time pcr in relation to stress and growth phase, four different strains of l. monocytogenes were exposed to combinations of osmotic stress (0.5 to 8% [vol/vol] nacl) and acid stress (ph 5 to 7) in a culture model at a growth temperature of 10 degrees c or were grown under optimal conditions. growth curves obtained from real-time pcr, optical density, and viable count data were compared. as expected, optical density data revealed entir ... | 2009 | 19181831 |
| insights into structure-activity relationships in the c-terminal region of divercin v41, a class iia bacteriocin with high-level antilisterial activity. | divercin v41 (dvnv41) is a class iia bacteriocin with potent antilisterial activity isolated from carnobacterium divergens v41. previously, we expressed from a synthetic gene, in escherichia coli origami, a recombinant dvnv41 designated dvnrv41, which possesses four additional amino acids (amdp) in the n-terminal region that result from enzymatic cleavage and retains the initial dvnv41 activity. to unravel the relationship between the structure of dvnrv41 and its particularly elevated activity, ... | 2009 | 19181835 |
| complete but curtailed t-cell response to very low-affinity antigen. | after an infection, t cells that carry the cd8 marker are activated and undergo a characteristic kinetic sequence of rapid expansion, subsequent contraction and formation of memory cells. the pool of naive t-cell clones is diverse and contains cells bearing t-cell antigen receptors (tcrs) that differ in their affinity for the same antigen. how these differences in affinity affect the function and the response kinetics of individual t-cell clones was previously unknown. here we show that during t ... | 2009 | 19182777 |
| in vitro and in vivo efficacies of amlodipine against listeria monocytogenes. | listeria monocytogenes causes suppurative gastritis in balb/c mice. we investigated the effect of the antihypertensive drug amlodipine (aml) on the growth of l. monocytogenes in vitro and in vivo. aml showed noteworthy inhibitory action (minimum inhibitory concentration, mic(90) 32 microg/ml) against listeria strains and demonstrated cidal (minimum bactericidal concentration, mbc 64 microg/ml) activity. aml administered orally at 2.5 microg/g in female balb/c mice for 7 days, commencing 4 days b ... | 2009 | 19184140 |
| [differentiantion of listeria monocytogenes strains isolated in the far east and european part of russia on the basis of polymorphism of genes encoding invasion factors]. | forty listeria monocytogenes isolates obtained in european and far east regions of russia were differentiated on the basis of polymorphism of 5 markergenes. total length of concatemers obtained after sequencing of internal fragments of genes inla, inlb, inlc, inle and prs was 3029 b.p. comparative analysis of concatemers' sequences revealed 237 variable nucleotides. totally, 25 sequence types were revealed, and isolates from european and far east regions belonged to different types. on the dendr ... | 2008 | 19186537 |
| [growth media for isolation, accumulation, and identification of listeria in laboratory diagnostics of mix-infections in women with obstetric-gynecological pathology]. | isolation of lysteria monocytogenes from clinical specimens in obstetric-gynecological pathology is often difficult owing to the presence of the mixed microbial flora. results of study of new russian growth media for isolation of listeria for microbiological diagnostics of listeriosis in pregnancy are presented. they show diagnostic efficacy of the developed media for laboratory diagnostics of mix-infections in women with obstetric-gynecological pathology. | 2008 | 19186555 |
| overcoming cancer immune tolerance and escape. | although her2/neu-targeted cancer vaccines have shown initial promise in the adjuvant setting, a therapeutic vaccine remains elusive due to the tumor escape mechanisms of established cancer. as described by seavey et al. in this issue of ccr, a listeria-delivered vaccine may help overcome immune tolerance, leading to an effective therapeutic vaccine. | 2009 | 19188142 |
| a novel human her-2/neu chimeric molecule expressed by listeria monocytogenes can elicit potent hla-a2 restricted cd8-positive t cell responses and impact the growth and spread of her-2/neu-positive breast tumors. | the aim of this study was to efficiently design a novel vaccine for human her-2/neu-positive (hher-2/neu) breast cancer using the live, attenuated bacterial vector listeria monocytogenes. | 2009 | 19188163 |
| prevention of autoimmune disease by induction of tolerance to toll-like receptor 7. | activation of toll-like receptors (tlr) contributes to the initiation and maintenance of chronic inflammation in autoimmune diseases, yet repeated exposure to a tlr agonist can induce hyporesponsiveness to subsequent tlr stimulation. here, we used a synthetic tlr7 agonist, 9-benzyl-8-hydroxy-2-(2-methoxyethoxy) adenine (sm360320, 1v136) to study tlr7 induced attenuation of inflammatory responses and its application to autoimmune diseases. repeated low dose administration of this tlr7 agonist ind ... | 2009 | 19188597 |
| listeria monocytogenes infection in the face of innate immunity. | pathogen recognition and induction of immune responses are important for efficient elimination of infection. however, pathogens such as listeria monocytogenes employ strategies to evade or modulate these defences, thus creating a more favourable environment that ensures their survival and pathogenesis. new insights into these strategies, particularly those targeting innate immunity, have recently emerged. l. monocytogenes is initially detected at the cell surface or in phagosomes by toll-like re ... | 2009 | 19191786 |
| listeria monocytogenes internalin and e-cadherin: from structure to pathogenesis. | many bacterial pathogens that invade non-phagocytic cells first interact with host cell surface receptors. adhesion to the host cell is followed by the activation of specific host signalling pathways that mediate bacterial internalization. the food-borne gram-positive bacterium listeria monocytogenes makes use of two surface proteins, internalin (inla) and inlb to engage in a species-specific manner the adhesion molecule e-cadherin and the hepatocyte growth factor receptor met, respectively, to ... | 2009 | 19191787 |
| cytotoxic t-lymphocyte antigen 4 blockade augments the t-cell response primed by attenuated listeria monocytogenes resulting in more rapid clearance of virulent bacterial challenge. | cytotoxic t-lymphocyte antigen 4 (ctla-4) uniformly suppresses antigen-specific t cells during chronic infection with bacterial, parasitic or viral pathogens. however, the importance of ctla-4 in controlling the t-cell response during acute infection or after priming with live attenuated vaccine vectors has not been well characterized. since strategies aimed at blocking ctla-4 are being actively developed to therapeutically augment t-cell-mediated immunity, the effects of ctla-4 blockade on t-ce ... | 2009 | 19191906 |
| combined tlr2 and tlr4 ligation in the context of bacterial or helminth extracts in human monocyte derived dendritic cells: molecular correlates for th1/th2 polarization. | recognition of pathogens by dendritic cells (dcs) through interaction with pattern recognition receptors, including toll like receptors (tlr), is crucial for the initiation of appropriate polarized t helper (th) cell responses. yet, the characteristics and differences in molecular profiles of dcs with different t cell polarizing capacities are still poorly defined. to address this issue, the molecular profile of human monocyte derived dcs was characterized after exposure to tlr4 ligand lps in co ... | 2009 | 19193240 |
| eight-plex pcr and liquid-array detection of bacterial and viral pathogens in cerebrospinal fluid from patients with suspected meningitis. | we here report on the development of a novel multiplex pcr with product detection in a luminex 100 suspension array system. the assay covers the nine most important bacterial and viral pathogens found in danish meningitis patients. the microorganisms include neisseria meningitidis, streptococcus pneumoniae, escherichia coli, staphylococcus aureus, listeria monocytogenes, streptococcus agalactiae, herpes simplex virus types 1 and 2, and varicella-zoster virus. the study was based on 1,187 samples ... | 2009 | 19193844 |
| hazard analysis of listeria monocytogenes contaminations in processing of salted roe from walleye pollock (theragra chalcogramma) in hokkaido, japan. | hazard analysis of listeria monocytogenes contamination during processing of salted walleye pollock (theragra chalcogramma) roe was performed for a seafood plant in japan from december 2005 to february 2006. as a result, l. monocytogenes number was detected on the pallet used for transport of barrels in the salting process and one of the rollers of the roller conveyor, which rotates while in contact with the bottoms of the barrels, but was not detected in any raw materials, interim products or f ... | 2009 | 19194081 |
| high initial frequency of tcr-transgenic cd8 t cells alters inflammation and pathogen clearance without affecting memory t cell function. | in adoptive transfer experiments, the initial frequency of naïve tcr-transgenic t cells impacts cd8 t cell phenotype after acute infections. the exact reasons for the observed changes, however, are unclear and it is unknown whether alterations in phenotype translate into impaired memory t cell function as well. here we perform in vivo comparisons of effector and memory cd8 t cells generated from high or low numbers of naïve precursors. we show that high numbers of adoptively transferred t cells ... | 2009 | 19195704 |
| [neonatal meningitis due to listeria monocytogenes after 3 weeks of maternal treatment during pregnancy]. | we report the case of a pregnant woman with listeriosis at 26 gestational weeks followed by premature labor at 30 gestational weeks. bacterial meningitis was suspected in the neonate with ventriculitis on sonography, a high level of protein in the cerebrospinal fluid (csf), and an identified specific bacterial genome of listeria monocytogenes (pcr 16s rdna and sequencing and specific amplification of l. monocytogenes hly gene) in csf. neonatal meningitis was complicated with cerebral venous sinu ... | 2009 | 19195853 |
| molecular characterization of the arginine deiminase system in listeria monocytogenes: regulation and role in acid tolerance. | the capacity of listeria monocytogenes to withstand low ph is important for growth in low-ph foods, successful passage through the gastric barrier and survival within the macrophage phagosome. the ability of this pathogen to survive and adapt to acidic conditions is therefore predicted to play a significant role in the infectious cycle. in silico analysis of the l. monocytogenes genome revealed the presence of putative arginine deiminase (adi) genes, which have been shown to play a role in the a ... | 2009 | 19196274 |
| antimicrobial activity of organically modified nano-clays. | antimicrobial activity of three kinds of commercially available montmorillonite nano-clays including a naturally occurring one (cloisite na+) and two organically modified ones (cloisite 20a and cloisite 30b) against four representative pathogenic bacteria (two gram-positive ones such as staphylococcus aureus and listeria monocytogenes, and two gram-negative ones such as salmonella typhimurium and e. coli o157:h7) was investigated. antimicrobial activity was found to be dependent on the type of n ... | 2008 | 19198311 |
| physical properties of gelidium corneum-gelatin blend films containing grapefruit seed extract or green tea extract and its application in the packaging of pork loins. | edible gelidium corneum-gelatin (gcg) blend films containing grapefruit seed extract (gfse) or green tea extract (gte) were manufactured, and the quality of pork loins packed with the film during storage was determined. tensile strength (ts) and water vapor permeability (wvp) of the films containing gfse or gte were better than those of the control. the film's antimicrobial activity against escherichia coli o157:h7 and listeria monocytogenes increased with increasing antimicrobial concentration, ... | 2009 | 19200087 |
| antimicrobial efficacy of zinc oxide quantum dots against listeria monocytogenes, salmonella enteritidis, and escherichia coli o157:h7. | zinc oxide quantum dots (zno qds) are nanoparticles of purified powdered zno. these were evaluated for antimicrobial activity against listeria monocytogenes, salmonella enteritidis, and escherichia coli o157:h7. the zno qds were utilized as a powder, bound in a polystyrene film (zno-ps), or suspended in a polyvinylprolidone gel (zno-pvp). bacteria cultures were inoculated into culture media or liquid egg white (lew) and incubated at 22 degrees c. the inhibitory efficacies of zno qds against 3 pa ... | 2009 | 19200107 |
| external concentration of organic acid anions and ph: key independent variables for studying how organic acids inhibit growth of bacteria in mildly acidic foods. | although the mechanisms by which organic acids inhibit growth of bacteria in mildly acidic foods are not fully understood, it is clear that intracellular accumulation of anions is a primary contributor to inhibition of bacterial growth. we hypothesize that intracellular accumulation of anions is driven by 2 factors, external anion concentration and external acidity. this hypothesis follows from basic chemistry principles that heretofore have not been fully applied to studies in the field, and it ... | 2009 | 19200113 |
| performance testing of six chromogenic aloa-type media for the detection of listeria monocytogenes. | the aim of the study was to test the performance of commercially available chromogenic plating media for detection and enumeration of the food-borne pathogen listeria monocytogenes. a wide range of chromogenic media similar to agar listeria according to ottaviani and agosti (aloa) were compared using palcam agar, according to van netten et al. | 2009 | 19200329 |
| isolation and identification of antibacterial compounds from thymus kotschyanus aerial parts and dianthus caryophyllus flower buds. | the aerial parts of thymus kotschyanus boiss. and hohen. (lamiaceae) and flower buds of dianthus caryophyllus l. (caryophyllaceae) have been traditionally implemented in the treatment of wounds, throat and gum infections and gastro-intestinal disorder by the indigenous people of northern iraq, although the compounds responsible for the medicinal properties have not been identified. in this study, antibacterial compounds from both plants were isolated and characterized, and the biological activit ... | 2009 | 19200700 |
| streptococcus-, shigella-, and listeria-induced autophagy. | autophagy has recently been described as an intrinsic host defense system for recognizing and eliminating intracellular-invading bacterial pathogens. some cytoplasmic-invading bacteria are trapped through the process of autophagy and are ultimately degraded within autolysosomal compartments. however, others exhibit highly evolved maneuvers for circumventing autophagic recognition and are capable of surviving and replicating within the cytoplasm. in this chapter, we describe bacterial infectious ... | 2009 | 19200893 |
| dynamic model of heat inactivation kinetics for bacterial adaptation. | the weibullian-log logistic (well) inactivation model was modified to account for heat adaptation by introducing a logistic adaptation factor, which rendered its "rate parameter" a function of both temperature and heating rate. the resulting model is consistent with the observation that adaptation is primarily noticeable in slow heat processes in which the cells are exposed to sublethal temperatures for a sufficiently long time. dynamic survival patterns generated with the proposed model were in ... | 2009 | 19201963 |
| mucosal vaccine using ctl epitope-pulsed dendritic cell confers protection for intracellular pathogen. | effective protective immunity against respiratory infections with intracellular pathogens requires pathogen-specific cytotoxic t cells (ctl) in the lung. however, vaccines that induce pathogen-specific ctl in the lung are poorly explored. dendritic cells (dc) have increasingly been exploited as vaccines against infections. however, few studies have investigated the ability of mucosal dc vaccines to elicit protective ctl responses in the lung. our objective was to develop an efficacious mucosal d ... | 2009 | 19202004 |
| multiple effector mechanisms induced by recombinant listeria monocytogenes anticancer immunotherapeutics. | listeria monocytogenes is a facultative intracellular gram-positive bacterium that naturally infects professional antigen presenting cells (apc) to target antigens to both class i and class ii antigen processing pathways. this infection process results in the stimulation of strong innate and adaptive immune responses, which make it an ideal candidate for a vaccine vector to deliver heterologous antigens. this ability of l. monocytogenes has been exploited by several researchers over the past dec ... | 2009 | 19203646 |
| inflammatory pseudotumor of the heart caused by listeria monocytogenes infection. | inflammatory pseudotumor (ipt) of the heart is rare and of unknown etiology. we present a case of cardiac ipt caused by listeria monocytogenes that evolved following gastroenteritis in a previously healthy child. l. monocytogenes, known to cause acute invasive infections, has not been reported previously as a cause of cardiac infection in children or of ipt. the literature concerning infectious ipt is reviewed. | 2009 | 19203798 |
| effects of ph and oil-in-water emulsions on growth and physicochemical cell surface properties of listeria monocytogenes: impact on tolerance to the bactericidal activity of disinfectants. | this study characterizes the effects of an acidic ph and an emulsified oil-in-water phase in a culture medium on the behavior of listeria monocytogenes. two strains were tested, scott a and cip 78.39, and exhibited similar responses to growth media. first of all, the results showed that the emulsified oil phase had no effect on growth kinetics, whereas acidification of the initial ph (from 7.2 to 5.2) reduced both growth rates and growth yields. secondly, physicochemical cell surface properties ... | 2009 | 19203811 |
| control of foodborne pathogens and soft-rot bacteria on bell pepper by three strains of bacterial antagonists. | forty-two representative strains of native bacteria associated with fresh peeled baby carrots were isolated and characterized. two of these strains, identified as pseudomonas fluorescens ag3a (pf ag3a) and bacillus yd1, were evaluated in conjunction with another known antagonist, p. fluorescens 2-79 (pf 2-79), for their potential as biocontrol agents of human pathogens (listeria monocytogenes, yersinia enterocolitica, salmonella enterica, and escherichia coli o157:h7) and soft-rot bacteria (erwi ... | 2009 | 19205468 |
| detection of listeria monocytogenes in commercially broken unpasteurized liquid egg in japan. | unpasteurized liquid whole-egg samples were collected from six and seven commercial establishments across japan in 1993 and 1994 and in 2005, respectively. the samples were tested for the presence of listeria spp. and listeria monocytogenes. detection rates of the listeria spp. were greatly different among the egg-breaking facilities, with a range of 8 to 55%. there was no significant difference in the contamination rate between the samples from 1993 and 1994 and from 2005. l. monocytogenes was ... | 2009 | 19205482 |
| [ventriculitis secondary to listeria monocytogenes]. | 2009 | 19206067 | |
| pathogen trafficking pathways and host phosphoinositide metabolism. | phosphoinositide (pi) glycerolipids are key regulators of eukaryotic signal transduction, cytoskeleton architecture and membrane dynamics. the host cell pi metabolism is targeted by intracellular bacterial pathogens, which evolved intricate strategies to modulate uptake processes and vesicle trafficking pathways. upon entering eukaryotic host cells, pathogenic bacteria replicate in distinct vacuoles or in the host cytoplasm. vacuolar pathogens manipulate pi levels to mimic or modify membranes of ... | 2009 | 19208094 |
| herpesvirus latency and symbiotic protection from bacterial infection. | 2009 | 19210221 | |
| gamma-herpesvirus-induced protection against bacterial infection is transient. | herpesviruses are widely disseminated in the population and establish lifelong latency, which is associated with a variety of pathological consequences. a recent report showed that mice latently infected with either murine gamma-herpesvirus-68 (gammahv68) or murine cytomegalovirus (mcmv), mouse pathogens genetically similar to the human herpesviruses, epstein-barr virus, kaposi's sarcoma-associated herpesvirus, and cytomegalovirus, had enhanced resistance to subsequent bacterial infection, sugge ... | 2009 | 19210230 |
| structural basis for autoinhibition and activation of auto, a virulence-associated peptidoglycan hydrolase of listeria monocytogenes. | during a bacterial infection, each successive step is orchestrated by a dedicated set of virulence factors. in gram-positive bacteria, the presentation or release of such factors is crucially dependent on the continual remodelling of the cell wall. we have investigated the autolysin or peptidoglycan hydrolase auto (lmo1076) from the human pathogen listeria monocytogenes to structurally and biochemically underpin its role in host cell invasion. we demonstrate that auto is an n-acetylglucosaminida ... | 2009 | 19210622 |
| expression of the reporter genes lacz and egfp in human glioblastoma cells using listeria monocytogenes as vector for gene transfer. | glioblastoma has become a model target for viral gene therapy approaches. to circumvent some of the inherent problems of viral vectors, we examined the feasibility of listeria monocytogenes for foreign gene expression in glioblastoma cells. | 2009 | 19215661 |
| characterization and partial purification of entomocin 110, a newly identified bacteriocin from bacillus thuringiensis subsp. entomocidus hd110. | a new bacteriocin produced by bacillus thuringiensis subsp. entomocidus was identified. the antibacterial activity termed entomocin 110 was produced starting at mid-logarithmic growth phase, reaching its maximum at the early and during stationary phase. the bacteriocin obtained from culture supernatant was inhibitory to several gram-positive bacteria including listeria monocytogenes, paenibacillus larvae and other bacillus species. entomocin 110 was shown to be heat stable and resistant to ph va ... | 2008 | 19216106 |
| comparative antimicrobial susceptibility of listeria monocytogenes, l. innocua, and l. welshimeri. | the current study compared antimicrobial susceptibility of listeria innocua, l. welshimeri, and l. monocytogenes isolated from various sources. antimicrobial susceptibility testing was performed using a microbroth procedure with sensititre minimum inhibitory concentration plates containing 18 antimicrobials. resistant isolates were analyzed for the presence of antimicrobial resistance genes using pcr. the majority of l. monocytogenes isolates were resistant to oxacillin (99%, 89/90) and ceftriax ... | 2009 | 19216646 |
| quantitative risk assessment of listeria monocytogenes in french cold-smoked salmon: ii. risk characterization. | a model for the assessment of exposure to listeria monocytogenes from cold-smoked salmon consumption in france was presented in the first of this pair of articles (pouillot et al., 2007, risk analysis, 27:683-700). in the present study, the exposure model output was combined with an internationally accepted hazard characterization model, adapted to the french situation, to assess the risk of invasive listeriosis from cold-smoked salmon consumption in france in a second-order monte carlo simulati ... | 2009 | 19220799 |
| interleukin-18-related genes are induced during the contraction phase but do not play major roles in regulating the dynamics or function of the t-cell response to listeria monocytogenes infection. | proinflammatory cytokines, such as gamma interferon (ifn-gamma), impact aspects of t-cell responses after infection, including expansion, contraction, and memory formation. interleukin-18 (il-18) functions as a proinflammatory cytokine by stimulating the production of ifn-gamma from multiple cell types and accentuating the development of th1 cd4 t-cell responses. focused microarray analyses revealed upregulation of il-18 and il-18 receptor genes in cd8 t cells during the contraction phase. based ... | 2009 | 19223481 |
| model systems allowing quantification of sensitivity to disinfectants and comparison of disinfectant susceptibility of persistent and presumed nonpersistent listeria monocytogenes. | to determine if listeria monocytogenes persistent strains differ from presumed nonpersistent strains in disinfection susceptibility and to examine the influence of attachment and nacl on susceptibility. | 2009 | 19226386 |
| screening and characterization of monoclonal antibodies to the surface antigens of listeria monocytogenes serotype 4b. | this study aims to develop and characterize monoclonal antibodies (mabs) with high specificity and affinity for surface antigens of an epidemiologically important serotype 4b of listeria monocytogenes. | 2009 | 19226395 |
| occurrence and distribution of listeria monocytogenes and other listeria species in ready-to-eat and raw meat products. | the present study was undertaken to estimate the occurrence and distribution of listeria monocytogenes and other listeria species in ready-to-eat food items (pasteurized milk, cheese, ice cream, and cakes) and raw meat products (minced beef, pork, and chicken carcasses). a total of 711 randomly selected samples were collected from supermarkets and pastry shops in addis ababa, ethiopia. listeria monocytogenes and other listeria species were isolated and identified according to the techniques reco ... | 2009 | 19226932 |
| listeriosis. | listeria monocytogenes, a small, facultative anaerobic, gram-positive motile bacillus, is an important cause of foodborne illness which disproportionately affects pregnant women and their newborns. listeria infects many types of animals and contaminates numerous foods including vegetables, milk, chicken and beef. this organism has a unique proclivity to infect the fetoplacental unit with the ability to invade cells, multiply intracellularly and be transmitted cell-to-cell. the organism possesses ... | 2009 | 19231307 |
| prevalence of low-virulence listeria monocytogenes strains from different foods and environments. | various studies have demonstrated variations in the levels of virulence of different l. monocytogenes strains. in our laboratory, a plaque-forming assay followed by subcutaneous footpad inoculation of mice enabled us to estimate the prevalence of the low-virulence strains. this value fell from 16.3% to 1.7% with bacteria collected before 1994 and after 1997 respectively. this could be related to the modification in 1997 of the reference method en iso 11 290-1 of listeria detection which recommen ... | 2009 | 19232447 |
| programming for cd8 t cell memory development requires il-12 or type i ifn. | inflammation can have both positive and negative effects on development of cd8 t cell memory, but the relative contributions and cellular targets of the cytokines involved are unclear. using cd8 t cells lacking receptors for il-12, type i ifn, or both, we show that these cytokines act directly on cd8 t cells to support memory formation in response to vaccinia virus and listeria monocytogenes infections. development of memory to vaccinia is supported predominantly by il-12, whereas both il-12 and ... | 2009 | 19234173 |
| selective priming and expansion of antigen-specific foxp3- cd4+ t cells during listeria monocytogenes infection. | the foxp3-expressing subset of regulatory cd4(+) t cells have defined ag specificity and play essential roles in maintaining peripheral tolerance by suppressing the activation of self-reactive t cells. similarly, during chronic infection, pathogen-specific foxp3-expressing cd4(+) t cells expand and actively suppress pathogen-specific effector t cells. herein, we used mhc class ii tetramers and foxp3(gfp) knockin mice to track the kinetics and magnitude whereby pathogen-specific foxp3(+)cd4(+) an ... | 2009 | 19234199 |
| septin 11 restricts inlb-mediated invasion by listeria. | septins are filament-forming gtpases implicated in several cellular functions, including cytokinesis. we previously showed that sept2, sept9, and sept11 colocalize with several bacteria entering into mammalian non-phagocytic cells, and sept2 was identified as essential for this process. here, we investigated the function of sept11, an interacting partner of sept9 whose function is still poorly understood. in uninfected hela cells, sept11 depletion by sirna increased cell size but surprisingly di ... | 2009 | 19234302 |
| transfer of plasmid-mediated resistance to tetracycline in pathogenic bacteria from fish and aquaculture environments. | the transferability of a large plasmid that harbors a tetracycline resistance gene tet(s), to fish and human pathogens was assessed using electrotransformation and conjugation. the plasmid, originally isolated from fish intestinal lactococcus lactis ssp. lactis kya-7, has potent antagonistic activity against the selected recipients (lactococcus garvieae and listeria monocytogenes), preventing conjugation. therefore the tetracycline resistance determinant was transferred via electroporation to l. ... | 2009 | 19236483 |
| stimulation of the molecule 4-1bb enhances host defense against listeria monocytogenes infection in mice by inducing rapid infiltration and activation of neutrophils and monocytes. | the tumor necrosis factor receptor family molecule 4-1bb (cd137) has diverse roles in adaptive and innate immune responses. however, little is known of its role in bacterial infections. previously, we showed that 4-1bb-deficient mice have enhanced susceptibility to listeria monocytogenes infection, and mice pretreated with agonistic anti-4-1bb antibody (3e1) were much more resistant to l. monocytogenes infection than mice treated with control antibody. in this study, we report that stimulating 4 ... | 2009 | 19237524 |
| identification and analysis of the osmotolerance associated genes in listeria monocytogenes. | listeria monocytogenes, the causative agent of listeriosis, has strong osmotolerance and is able to grow in severe circumstances. many studies of the mechanisms of listerial osmotolerance have been performed. however, there is much which remains unknown. in previous studies we constructed two kinds of mutant in l. monocytogenes egd strain to analyse the mechanisms of osmotolerance in l. monocytogenes by molecular genetic methods. in this paper, we summarized the genetical studies of osmotoleranc ... | 2008 | 19238619 |
| sudden increases in listeriosis rates in england and wales, 2001 and 2003. | the monthly incidence of listeriosis infections in england and wales had 2 sudden increases during april 2001 (41%) and march 2003 (48%). although no causative association is demonstrated, these increases correspond to key dates relating to the onset and aftermath of the 2001 foot and mouth disease outbreak in the united kingdom. | 2009 | 19239766 |
| development of a hierarchical bayesian model to estimate the growth parameters of listeria monocytogenes in minimally processed fresh leafy salads. | the optimal growth rate mu(opt) of listeria monocytogenes in minimally processed (mp) fresh leafy salads was estimated with a hierarchical bayesian model at (mean+/-standard deviation) 0.33+/-0.16 h(-1). this mu(opt) value was much lower on average than that in nutrient broth, liquid dairy, meat and seafood products (0.7-1.3 h(-1)), and of the same order of magnitude as in cheese. cardinal temperatures t(min), t(opt) and t(max) were determined at -4.5+/-1.3 degrees c, 37.1+/-1.3 degrees c and 45 ... | 2009 | 19239977 |
| effect of whey protein coating on quality attributes of low-fat, aerobically packaged sausage during refrigerated storage. | whey protein-based edible coating was used to reduce oxidative degradation and microbial growth of low-fat sausages (lfss) stored at 4 degrees c for 8 wk, under aerobic package. whey protein coating reduced (p<0.05) thiobarbituric acid-reactive substances (tbars) and peroxide value (pv) formation compared to control sausages. the percent inhibition of tbars and pv for whey protein-coated sausages, compared to the control, was 31.3% and 27.1%, respectively. the ability of the whey protein coating ... | 2008 | 19241536 |
| modeling surface transfer of listeria monocytogenes on salami during slicing. | listeria monocytogenes has been implicated in several listeriosis outbreaks linked to the consump tion of presliced ready-to-eat (rte) deli meats, which has drawn considerable attention in regard to possible cross-contamination during slicing operation at retail and food service environments. salami with 15% fat (a moderate l fat content deli item) was used to investigate the transfer of l. monocytogenes between a meat slicer and salami slices and to understand its impact on food safety. a 6-str ... | 2008 | 19241551 |
| effect of electrolyzed water for reduction of foodborne pathogens on lettuce and spinach. | the ability of electrolyzed water (ew) to inactivate foodborne pathogens on the surfaces of lettuce and spinach was investigated. lettuce and spinach leaves were inoculated with a cocktail of 3 strains each of escherichia col o157:h7, salmnonella typhimurium, and listeria monocytogenes and treated with acidic electrolyzed water (ac-ew), alkaline electrolyzed water (ak-ew), alkaline electrolyzed water followed by acidic electrolyzed water (sequential treatment, ak-ew + ac-ew), deionized water fol ... | 2008 | 19241556 |
| microbial assessment in school foodservices and recommendations for food safety improvement. | this study evaluated microbial food safety in school foodservices. five school foodservices were randomly selected, and samples from water, cooking utensils, tableware, foodservice surroundings, and linen were collected in summer and winter (n=420). tap and drinking water samples were collected, samples of food contact surfaces were collected by swab-kit, and samples for foodservice workers' hands and gloves were prepared by glove juice method. aerobic plate count (apc) and coliform bacterial po ... | 2008 | 19241563 |
| prevalence of listeria spp including listeria monocytogenes from apparently healthy sheep of gujarat state, india. | a total of 1002 samples comprising blood (n = 248), faecal swabs (n = 248), nasal swabs (n = 248) and deep vaginal swabs (n = 248) collected from the 248 sheep and 10 environmental samples of 10 sheep flocks were examined for the presence of pathogenic listeria spp. confirmation of the isolates was based on biochemical tests followed by phenotypic characterization by haemolysis on sheep blood agar, christie atkins munch-petersen (camp) test phosphatidylinositol-specific phospholipase c (pi-plc) ... | 2009 | 19243567 |
| coliforms and prevalence of escherichia coli and foodborne pathogens on minimally processed spinach in two packing plants. | minimally processed spinach has been recently associated with outbreaks of foodborne illnesses. this study investigated the effect of commercial minimal processing of spinach on the coliform and escherichia coli counts and the prevalence of e. coli o157:h7, salmonella, shigella spp., and listeria monocytogenes on two types of spinach before and after minimal processing. a total of 1,356 spinach samples (baby spinach, n = 574; savoy spinach, n = 782) were collected daily in two processing plants ... | 2008 | 19244890 |
| inactivation of listeria monocytogenes in raw fruits by enterocin as-48. | the purpose of this study was to determine the effect of enterocin as-48 on listeria monocytogenes cect 4032 in fruits and fruit juice. fruits were contaminated with a l. monocytogenes cell suspension, washed with enterocin as-48 (25 microg/ml) or with sterile distilled water as control, and stored at different temperatures (-20, 6, 15, 22 degrees c). washing treatments significantly inhibited or completely inactivated l. monocytogenes in strawberries, raspberries, and blackberries stored at 15 ... | 2008 | 19244899 |
| comparison of antimicrobial resistance in escherichia coli, staphylococcus aureus, and listeria monocytogenes strains isolated from organic and conventional poultry meat. | the presence of escherichia coli, staphylococcus aureus, and listeria monocytogenes was determined in 55 samples of organic poultry meat and in 61 samples of conventional poultry meat. a total of 220 e. coli, 192 s. aureus, and 71 l. monocytogenes strains were analyzed by an agar disk diffusion assay for their resistance to ampicillin, cephalothin, chloramphenicol, ciprofloxacin, doxycycline, fosfomycin, gentamicin, nitrofurantoin, streptomycin, and sulfisoxazole (e. coli); chloramphenicol, cipr ... | 2008 | 19244911 |
| analysis of additional virulence genes and virulence gene regions in listeria monocytogenes confirms the epidemiologic relevance of multi-virulence-locus sequence typing. | previous molecular subtyping studies have defined four epidemic clones (ecs) of listeria monocytogenes (eci, ecii, eciii, and eciv). partial sequences of eight virulence genes were previously shown to be identical within individual ecs of l. monocytogenes. the present study was conducted to determine if the sequences of other virulence genes and virulence gene regions are also conserved within these ecs. six additional virulence genes--bsh, hly, inlj, ipla1, pgda, and srta--and three additional ... | 2008 | 19244915 |
| assessment of safety, nutritional, and spoilage characteristics of different lagoon grey mullets (liza ramada, liza aurata, and liza saliens). | different lagoon grey mullets such as liza ramada (thinlip mullet), liza aurata (golden grey mullet), and liza saliens (leaping grey mullet) were analyzed for their nutritional, microbiological, and safety parameters. the microbiological values never exceeded the lower limits stipulated by the italian higher institute of health. the pathogenic species frequently associated with seafood (salmonella, listeria monocytogenes, vibrio cholerae, vibrio parahaemolyticus, and aeromonas hydrophila) were n ... | 2008 | 19244917 |
| lmo1273, a novel gene involved in listeria monocytogenes virulence. | listeria monocytogenes is a foodborne pathogen able to infect humans and many other mammalian species, leading to serious, often fatal disease. we have previously identified a five-gene locus in the genome of l. monocytogenes egd-e which comprised three contiguous genes encoding paralogous type i signal peptidases. in the present study, we focused on the two distal genes of the locus (lmo1272 and lmo1273), encoding proteins sharing significant similarities with the ylqf and rnhb proteins, respec ... | 2009 | 19246760 |
| functional properties of novel protective lactic acid bacteria and application in raw chicken meat against listeria monocytogenes and salmonella enteritidis. | in this study 635 lactic acid bacteria of food origin were evaluated for their potential application as protective cultures in foods. a stepwise selection method was used to obtain the most appropriate strains for application as protective cultures in chicken meat. specifically, all strains were examined for antimicrobial activity against various gram positive and gram negative pathogenic and spoilage bacteria. strains exhibiting anti-bacterial activity were subsequently examined for survival in ... | 2009 | 19249112 |
| inactivated parapoxvirus ovis activates canine blood phagocytes and t lymphocytes. | inactivated parapoxvirus ovis (ippvo) shows strong immunomodulatory activities in several species and is used in veterinary medicine as an immunostimulatory biological for the prevention and/or treatment of infectious diseases. in this study the immunostimulatory capacity of ippvo on the innate immune system was investigated in vitro by the evaluation of induction of the oxidative burst and modulation of phagocytosis by canine blood leukocytes (polymorphonuclear cells and monocytes) of dogs. in ... | 2009 | 19251383 |
| temperature-dependent phage resistance of listeria monocytogenes epidemic clone ii. | listeria monocytogenes epidemic clone ii (ecii) has been responsible for two multistate outbreaks in the united states in 1998-1999 and in 2002, in which contaminated ready-to-eat meat products (hot dogs and turkey deli meats, respectively) were implicated. however, ecological adaptations of ecii strains in the food-processing plant environment remain unidentified. in this study, we found that broad-host-range phages, including phages isolated from the processing plant environment, produced plaq ... | 2009 | 19251898 |
| isolation, purification and de novo sequencing of tbd-1, the first beta-defensin from leukocytes of reptiles. | a novel peptide with antimicrobial activity was isolated from leukocytes of the european pond turtle emys orbicularis and purified to homogeneity by preparative gel electrophoresis followed by reversed phase chromatography. it was highly active in vitro against escherichia coli, listeria monocytogenes, methicillin-resistant staphylococcus aureus, and candida albicans. the isolated peptide was sequenced de novo by tandem mass spectrometry using both collision-induced and electron-transfer dissoci ... | 2009 | 19253295 |
| identification of potential on-farm sources of listeria monocytogenes in herds of dairy cattle. | to elucidate the ecology of listeria monocytogenes on dairy cattle farms by determining the prevalence of the organism in various samples. | 2009 | 19254151 |
| listeria monocytogenes sigmab modulates prfa-mediated virulence factor expression. | listeria monocytogenes sigma(b) and positive regulatory factor a (prfa) are pleiotropic transcriptional regulators that coregulate a subset of virulence genes. a positive regulatory role for sigma(b) in prfa transcription has been well established; therefore, observations of increased virulence gene expression and hemolytic activity in a deltasigb strain initially appeared paradoxical. to test the hypothesis that l. monocytogenes sigma(b) contributes to a regulatory network critical for appropri ... | 2009 | 19255187 |
| [microbiological contamination and antimicrobial activity of cristalised cane sugar on some medically important microorganisms in costa rica]. | microbiological contamination and antimicrobial activity of cristalised cane sugar on some medically important microorganisms in costa rica. unrefined cristalised cane sugar, obtained after the filtration and evaporation of sugar cane juice, is a nutritional product of traditional consumption in costa rica and other neotropical countries. it has been used in the topic treatment of infected wounds, with satisfactory results even with some antibiotic-ressistant bacteria. we studied the microbiolog ... | 2008 | 19256417 |
| intracellular bacterial infection and invariant nkt cells. | the invariant (i) natural killer (nk)t cells represent a unique subset of t lymphocytes which express the v alpha 14 chain of the t cell receptor (tcr), that recognizes glycolipid antigens presented by the nonpolymorphic major histocompatibility complex (mhc) class i-like antigen presentation molecule cd1d, and they participate in protection against some microbial pathogens. although inkt cells have originally been regarded as t cells co-expressing nkr-p1b/c (nk1.1: cd 161), they do not seem to ... | 2009 | 19259343 |